Rw4G029370

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014499
Physical Location & Seq
Reverse (-)
56028542 .. 56030178
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029370.1

Sequence Viewer

Length: 846 bp
ATGGGAAGTGGCAGCCTGGTCCGGATAGTTCCTTTGTGTTCACTGCTTCTTCTTCTTCTTTTTCCTGTGATTCATGGTTGGGGTATCGAGGGTCACACGACGGTGTGCAGAATTGCTCAGTCTAGGTTGAGCGATGCGGCAGCAGATGCAGTGAAGCAATTGCTTCCCAAGTATGCAGAAAATGACTTGGGGACTGTGTGCATATGGGCTGATCGTGTCAAGTTTCGGTATCACTGGTCATCTGCCCTTCACTACATTGACACTCCTGACAACCTTTGCACTTATCAGTACAATAGGGACTGCAAGGACGAAAATGGAGACAAGGGGCGTTGTGTTGCAGGGGCCATAAACAATTACACCACTCAGCTTCTCACTTACAGCTCCGGTTCTTCCAAGTCGGAATATAACCTCACTGAAGCACTGTTATTCCTTTCTCATTTTTTCGGAGACATCCATCAGCCTCTACATGTGGGTTTCACTTCAGACAGGGGGGGCAATACCATTGAAGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAAACAGCAGAAGAGAGCTTCTACGAGAATAATCTGGATGAGCTGGTTGATGCGATTAAACAGAACATTACGACTGAATGGGCAGATGAAGTTAAGGCTTGGGAGCACACTATGAAAGCTATTAGCCGTATCAAAGCAGCTTGTGATTGGGCTTACAAGGGTGTAGACGAAGGCTCAGTCTTACAAGATCCATACTTCCTCTCCCGGTTACCTATCGTCAATAGGCGGTTGGCTCAAGGTGGAGTCCGGTTAGCAGCCACTCTCAACCGGATCTTCGGTTGA

Protein Analysis

281

Amino Acids

31.76

Weight (kDa)

5.99

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 27 - 280 2.1e-81 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68290
fragaria_vesca FvH4_4g27190
malus_domestica MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica Prupe.1G312700_v2.0.a1
pyrus_communis pycom16g03580
rosa_chinensis RchiOBHm_Chr4g0434991
rosa_laevigata RLG00000006631
rosa_multiflora Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii Rroxscaffold_5G00376160
rosa_rugosa Rorug04G0281500 Rorug04G0281500
rosa_samantha Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana Rw4G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 729
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 2 cut(s) 137, 790
AclWI GGATC 2 cut(s) 746, 842
AcuI CTGAAG 2 cut(s) 435, 465
AfaI GTAC 2 cut(s) 290, 518
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 1 cut(s) 506
AjnI CCWGG 2 cut(s) 15, 521
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 6 cut(s) 367, 381, 582, 607, 683, 704
AluI AGCT 6 cut(s) 367, 381, 582, 607, 683, 704
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 2 cut(s) 312, 441
AlwI GGATC 2 cut(s) 746, 842
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 4 cut(s) 12, 140, 701, 818
ArsI GACNNNNNNTTYG 2 cut(s) 726, 758
AspS9I GGNCC 2 cut(s) 19, 342
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 1 cut(s) 530
AvaII GGWCC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 24, 152, 713, 830
BccI CCATC 1 cut(s) 462
BceAI ACGGC 1 cut(s) 675
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 17, 523
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 2 cut(s) 312, 441
BfaI CTAG 1 cut(s) 123
BisI GCNGC 5 cut(s) 13, 138, 141, 702, 819
BlsI GCNGC 5 cut(s) 14, 139, 142, 703, 820
Bme1390I CCNGG 3 cut(s) 17, 523, 769
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 342
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 3 cut(s) 17, 523, 769
BmsI GCATC 3 cut(s) 124, 136, 604
BpuEI CTTGAG 1 cut(s) 783
BpuMI CCSGG 1 cut(s) 769
BsaWI WCCGGW 4 cut(s) 21, 383, 810, 831
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 239, 518
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 2 cut(s) 17, 523
BseGI GGATG 2 cut(s) 450, 607
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 3 cut(s) 131, 377, 753
BseNI ACTGG 2 cut(s) 239, 518
BseXI GCAGC 4 cut(s) 24, 152, 713, 830
BsgI GTGCAG 1 cut(s) 127
BshFI GGCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 5 cut(s) 22, 384, 769, 811, 832
BslFI GGGAC 2 cut(s) 205, 311
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 312, 441
BsmFI GGGAC 2 cut(s) 205, 311
BsnI GGCC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 672
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 3 cut(s) 211, 751, 834
BspACI CCGC 2 cut(s) 137, 790
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 3 cut(s) 130, 376, 752
BspEI TCCGGA 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 343
BspPI GGATC 2 cut(s) 746, 842
BsrI ACTGG 2 cut(s) 239, 518
BssMI GATC 3 cut(s) 211, 751, 834
Bst2UI CCWGG 2 cut(s) 17, 523
Bst4CI ACNGT 3 cut(s) 103, 196, 423
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 146
BstDEI CTNAG 3 cut(s) 117, 363, 739
BstEII GGTNACC 1 cut(s) 771
BstF5I GGATG 2 cut(s) 450, 607
BstKTI GATC 3 cut(s) 214, 754, 837
BstMAI GTCTC 2 cut(s) 312, 441
BstMBI GATC 3 cut(s) 211, 751, 834
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 17, 523
BstNSI RCATGY 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 771
BstSCI CCNGG 3 cut(s) 15, 521, 767
BstV1I GCAGC 4 cut(s) 24, 152, 713, 830
BstX2I RGATCY 2 cut(s) 751, 834
BstYI RGATCY 2 cut(s) 751, 834
BsuRI GGCC 1 cut(s) 344
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 450, 607
BtsI GCAGTG 2 cut(s) 41, 156
BtsIMutI CAGTG 6 cut(s) 41, 156, 232, 411, 419, 511
Cfr13I GGNCC 2 cut(s) 19, 342
Csp6I GTAC 2 cut(s) 289, 517
CviAII CATG 2 cut(s) 74, 467
CviQI GTAC 2 cut(s) 289, 517
DdeI CTNAG 3 cut(s) 117, 363, 739
DpnI GATC 3 cut(s) 213, 753, 836
DpnII GATC 3 cut(s) 211, 751, 834
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 435, 465
Eco91I GGTNACC 1 cut(s) 771
EcoO65I GGTNACC 1 cut(s) 771
EcoRII CCWGG 2 cut(s) 15, 521
FaeI CATG 2 cut(s) 77, 470
FaqI GGGAC 2 cut(s) 205, 311
FatI CATG 2 cut(s) 73, 466
FauNDI CATATG 1 cut(s) 203
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 5 cut(s) 13, 138, 141, 702, 819
FokI GGATG 2 cut(s) 437, 614
Fsp4HI GCNGC 5 cut(s) 13, 138, 141, 702, 819
FspBI CTAG 1 cut(s) 123
GluI GCNGC 5 cut(s) 13, 138, 141, 702, 819
HaeIII GGCC 1 cut(s) 344
HapII CCGG 5 cut(s) 22, 384, 769, 811, 832
Hin1II CATG 2 cut(s) 77, 470
HinfI GANTC 2 cut(s) 70, 807
HpaII CCGG 5 cut(s) 22, 384, 769, 811, 832
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 4 cut(s) 41, 511, 519, 730
Hpy188I TCNGA 3 cut(s) 400, 446, 484
Hpy188III TCNNGA 3 cut(s) 22, 266, 599
Hpy8I GTNNAC 4 cut(s) 41, 511, 519, 730
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 257, 728
HpyCH4III ACNGT 3 cut(s) 103, 196, 423
HpyCH4V TGCA 7 cut(s) 108, 149, 176, 201, 279, 303, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 117, 363, 739
Hsp92II CATG 2 cut(s) 77, 470
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 3 cut(s) 211, 751, 834
LmnI GCTCC 2 cut(s) 386, 667
Lsp1109I GCAGC 4 cut(s) 24, 152, 713, 830
LweI GCATC 3 cut(s) 124, 136, 604
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 92, 771
MalI GATC 3 cut(s) 213, 753, 836
MboI GATC 3 cut(s) 211, 751, 834
MboII GAAGA 7 cut(s) 41, 44, 47, 381, 527, 587, 829
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 2 cut(s) 751, 834
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 3 cut(s) 111, 158, 352
MlyI GAGTC 1 cut(s) 816
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 4 cut(s) 82, 419, 471, 773
MroI TCCGGA 1 cut(s) 21
MseI TTAA 2 cut(s) 621, 657
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 5 cut(s) 22, 384, 769, 811, 832
MspR9I CCNGG 3 cut(s) 17, 523, 769
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 17, 523
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 1 cut(s) 769
NdeI CATATG 1 cut(s) 203
NdeII GATC 3 cut(s) 211, 751, 834
NlaIII CATG 2 cut(s) 77, 470
NlaIV GGNNCC 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 92
NspI RCATGY 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 101
PciI ACATGT 1 cut(s) 466
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 5 cut(s) 14, 139, 142, 703, 820
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
PscI ACATGT 1 cut(s) 466
Psp6I CCWGG 2 cut(s) 15, 521
PspEI GGTNACC 1 cut(s) 771
PspGI CCWGG 2 cut(s) 15, 521
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 2 cut(s) 19, 342
PsuI RGATCY 2 cut(s) 751, 834
RsaI GTAC 2 cut(s) 290, 518
RsaNI GTAC 2 cut(s) 289, 517
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 2 cut(s) 621, 657
SatI GCNGC 5 cut(s) 13, 138, 141, 702, 819
Sau3AI GATC 3 cut(s) 211, 751, 834
Sau96I GGNCC 2 cut(s) 19, 342
SchI GAGTC 1 cut(s) 816
ScrFI CCNGG 3 cut(s) 17, 523, 769
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 3 cut(s) 124, 136, 604
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
Sse9I AATT 3 cut(s) 111, 158, 352
SsiI CCGC 2 cut(s) 137, 790
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 15, 521, 767
TaaI ACNGT 3 cut(s) 103, 196, 423
TaqI TCGA 2 cut(s) 87, 564
TasI AATT 3 cut(s) 111, 158, 352
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 2 cut(s) 621, 657
Tru9I TTAA 2 cut(s) 621, 657
TscAI CASTG 6 cut(s) 48, 156, 239, 418, 426, 518
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 4 cut(s) 12, 140, 701, 818
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 3 cut(s) 62, 666, 692
TspRI CASTG 6 cut(s) 48, 156, 239, 418, 426, 518
VpaK11BI GGWCC 1 cut(s) 19
XceI RCATGY 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.