RchiOBHm_Chr4g0434991

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014499
Physical Location & Seq
Reverse (-)
58587600 .. 58589117
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40343

Sequence Viewer

Length: 648 bp
ATGGGAAGTGGCAGCCTGGTCCGGATAGTTCCTATGTGTTCACTGCTTCTTCTTCTTCTTTTTCCTGTGATTCATGGTTGGGGTATCGAGGGTCACACGACGGTGTGCAGAATTGCTCAGTCTAGGTTGAGCGATGCGGCAGCAGATGCAGTGAAGCAATTGCTTCCCAAGTATGCAGAAAATGACTTGGGGACTGTGTGCATATGGGCCGATCGTGTCAAGTTTCGGTATCACTGGTCATCTGCCCTTCACTACATTGACACTCCTGACAACCTTTGCACTTATCAGTACAATAGGGACTGCAAGGACGAAAATGGAGACAAGGGGCGTTGTGTTGCAGGGGCCATAAACAATTACACCACTCAGCTTCTCACTTACAGCTCCGGTTCTTCCAAGTCGGAATATAACCTCACTGAAGCACTGTTATTCCTTTCTCATTTTTTCGGAGACATCCATCAGCCTCTACATGTGGGTTTCACTTCAGACAGGGGGGGCAATACCATTGAAGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAAACAGCAGAAGAGAGGTTCTACGAGAATAATCTGGATGAGCTGGTTGATGCGATTAAACAGAACATTACGGTACTATCTTAG

Protein Analysis

215

Amino Acids

24.37

Weight (kDa)

5.76

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 27 - 212 9.7e-63 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68290
fragaria_vesca FvH4_4g27190
malus_domestica MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica Prupe.1G312700_v2.0.a1
pyrus_communis pycom16g03580
rosa_chinensis RchiOBHm_Chr4g0434991
rosa_laevigata RLG00000006631
rosa_multiflora Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii Rroxscaffold_5G00376160
rosa_rugosa Rorug04G0281500 Rorug04G0281500
rosa_samantha Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana Rw4G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 1 cut(s) 137
AcuI CTGAAG 2 cut(s) 435, 465
AfaI GTAC 3 cut(s) 290, 518, 639
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 1 cut(s) 506
AjnI CCWGG 2 cut(s) 15, 521
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 3 cut(s) 367, 381, 607
AluI AGCT 3 cut(s) 367, 381, 607
Alw26I GTCTC 2 cut(s) 312, 441
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 2 cut(s) 207, 342
ApeKI GCWGC 2 cut(s) 12, 140
AspS9I GGNCC 3 cut(s) 19, 207, 342
AsuHPI GGTGA 1 cut(s) 530
AvaII GGWCC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 24, 152
BccI CCATC 1 cut(s) 462
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 17, 523
BcoDI GTCTC 2 cut(s) 312, 441
BfaI CTAG 1 cut(s) 123
BisI GCNGC 3 cut(s) 13, 138, 141
BlsI GCNGC 3 cut(s) 14, 139, 142
Bme1390I CCNGG 2 cut(s) 17, 523
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 3 cut(s) 19, 207, 342
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 2 cut(s) 17, 523
BmsI GCATC 3 cut(s) 124, 136, 604
BsaWI WCCGGW 2 cut(s) 21, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 239, 518
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 2 cut(s) 17, 523
BseGI GGATG 2 cut(s) 450, 607
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 2 cut(s) 131, 377
BseNI ACTGG 2 cut(s) 239, 518
BseXI GCAGC 2 cut(s) 24, 152
BsgI GTGCAG 1 cut(s) 127
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 209, 344
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 2 cut(s) 22, 384
BslFI GGGAC 2 cut(s) 205, 311
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 312, 441
BsmFI GGGAC 2 cut(s) 205, 311
BsnI GGCC 2 cut(s) 209, 344
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 1 cut(s) 211
BspACI CCGC 1 cut(s) 137
BspANI GGCC 2 cut(s) 209, 344
BspCNI CTCAG 2 cut(s) 130, 376
BspEI TCCGGA 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 343
BsrI ACTGG 2 cut(s) 239, 518
BssMI GATC 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 17, 523
Bst4CI ACNGT 4 cut(s) 103, 196, 423, 637
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 146
BstDEI CTNAG 3 cut(s) 117, 363, 645
BstF5I GGATG 2 cut(s) 450, 607
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 2 cut(s) 312, 441
BstMBI GATC 1 cut(s) 211
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 17, 523
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 15, 521
BstV1I GCAGC 2 cut(s) 24, 152
BsuRI GGCC 2 cut(s) 209, 344
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 450, 607
BtsI GCAGTG 2 cut(s) 41, 156
BtsIMutI CAGTG 6 cut(s) 41, 156, 232, 411, 419, 511
Cfr13I GGNCC 3 cut(s) 19, 207, 342
Csp6I GTAC 3 cut(s) 289, 517, 638
CviAII CATG 2 cut(s) 74, 467
CviJI RGCY 7 cut(s) 15, 209, 344, 367, 381, 460, 607
CviKI_1 RGCY 7 cut(s) 15, 209, 344, 367, 381, 460, 607
CviQI GTAC 3 cut(s) 289, 517, 638
DdeI CTNAG 3 cut(s) 117, 363, 645
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 435, 465
EcoRII CCWGG 2 cut(s) 15, 521
FaeI CATG 2 cut(s) 77, 470
FaqI GGGAC 2 cut(s) 205, 311
FatI CATG 2 cut(s) 73, 466
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 3 cut(s) 13, 138, 141
FokI GGATG 2 cut(s) 437, 614
Fsp4HI GCNGC 3 cut(s) 13, 138, 141
FspBI CTAG 1 cut(s) 123
GluI GCNGC 3 cut(s) 13, 138, 141
HaeIII GGCC 2 cut(s) 209, 344
HapII CCGG 2 cut(s) 22, 384
Hin1II CATG 2 cut(s) 77, 470
HinfI GANTC 1 cut(s) 70
HpaII CCGG 2 cut(s) 22, 384
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 3 cut(s) 41, 511, 519
Hpy188I TCNGA 3 cut(s) 400, 446, 484
Hpy188III TCNNGA 3 cut(s) 22, 266, 599
Hpy8I GTNNAC 3 cut(s) 41, 511, 519
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 4 cut(s) 103, 196, 423, 637
HpyCH4V TGCA 7 cut(s) 108, 149, 176, 201, 279, 303, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 117, 363, 645
Hsp92II CATG 2 cut(s) 77, 470
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 2 cut(s) 24, 152
LweI GCATC 3 cut(s) 124, 136, 604
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 92
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 6 cut(s) 41, 44, 47, 381, 527, 587
MfeI CAATTG 1 cut(s) 158
MluCI AATT 3 cut(s) 111, 158, 352
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 4 cut(s) 82, 419, 471, 573
MroI TCCGGA 1 cut(s) 21
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 2 cut(s) 22, 384
MspR9I CCNGG 2 cut(s) 17, 523
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 17, 523
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeI CATATG 1 cut(s) 203
NdeII GATC 1 cut(s) 211
NlaIII CATG 2 cut(s) 77, 470
NlaIV GGNNCC 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 92
NspI RCATGY 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 101
PciI ACATGT 1 cut(s) 466
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 3 cut(s) 14, 139, 142
Ple19I CGATCG 1 cut(s) 214
PscI ACATGT 1 cut(s) 466
Psp6I CCWGG 2 cut(s) 15, 521
PspGI CCWGG 2 cut(s) 15, 521
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 3 cut(s) 19, 207, 342
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 3 cut(s) 290, 518, 639
RsaNI GTAC 3 cut(s) 289, 517, 638
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 13, 138, 141
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 3 cut(s) 19, 207, 342
ScrFI CCNGG 2 cut(s) 17, 523
SetI ASST 7 cut(s) 128, 276, 369, 383, 411, 584, 609
SfaNI GCATC 3 cut(s) 124, 136, 604
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 101
Sse9I AATT 3 cut(s) 111, 158, 352
SsiI CCGC 1 cut(s) 137
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 15, 521
TaaI ACNGT 4 cut(s) 103, 196, 423, 637
TaqI TCGA 2 cut(s) 87, 564
TasI AATT 3 cut(s) 111, 158, 352
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 6 cut(s) 48, 156, 239, 418, 426, 518
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 2 cut(s) 12, 140
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 62
TspRI CASTG 6 cut(s) 48, 156, 239, 418, 426, 518
VpaK11BI GGWCC 1 cut(s) 19
XceI RCATGY 1 cut(s) 470
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.