RLG00000006631

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014499
Physical Location & Seq
Forward (+)
9205354 .. 9207008
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006631

Sequence Viewer

Length: 882 bp
ATGGAAAGTGGCAGCCTGGTCCGGATAGTTCCTTTGTGTTCACTGCTTCTTCTTCTTCTTCTTCTTCTTTTTCCTGTGAGTCATGGTTGGGGTATCGAGGGTCACACAACGGTGTGTAGAATTGCTCAGTCTAGGTTGAGCGATGCGGCAGCAGATGCAGTGAAGCAATTGCTTCCCAAGTATGCAGAAAATGACTTGGGGACTGTGTGCATATGGGCCGATCGTGTCAAGTTTCGGTATCACTGGTCATCTGCCCTTCACTACATTGACACTCCTGACAACCTTTGCACTTATCAGTACACTAGGGACTGCAAGGACGAAAATGGAGACAAGGGGCGTTGTGTTGCAGGGGCCATAAACAATTACACCACTCAACTTCTCACTTACAACTCCGGTTCTTCCAAGTCGGAATATAACCTCACTGAAGCACTGTTATTCCTTTCTCACTTTTTCGGAGACATCCATCAGCCTCTACATGTGGGTTTCACTTCAGACAGGGGGGCCAATACCATTGAAGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAAACAGCAGAAGAGAGGTTCTACGAGAATAATCTGGATGAGCTGGTTGATGCGATTAAACAGAACATTACGACTGAATGGGCAGATGAAGTTAAGGCTTGGGAGACCTGCAGTTTGAACAAGACAGCATGTCCAGATGTATATGCATCTGAAGGTATCAAAGCAGCTTGTGATTGGGCTTACAAGGGTGTAGACGACGGCTCAGTCTTACAAGATCCATACTTCCTCTCCCGGTTACCTATCGTCAATAGGCGGTTGGCTCAAGGTGGAGTCCGGTTAGCAGCCACTCTCAACCGGATCTTCGGTTGA

Protein Analysis

294

Amino Acids

33.04

Weight (kDa)

5.56

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 30 - 292 2.1e-84 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68290
fragaria_vesca FvH4_4g27190
malus_domestica MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica Prupe.1G312700_v2.0.a1
pyrus_communis pycom16g03580
rosa_chinensis RchiOBHm_Chr4g0434991
rosa_laevigata RLG00000006631
rosa_multiflora Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii Rroxscaffold_5G00376160
rosa_rugosa Rorug04G0281500 Rorug04G0281500
rosa_samantha Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana Rw4G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 776
Acc36I ACCTGC 1 cut(s) 689
AccI GTMKAC 1 cut(s) 765
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 2 cut(s) 146, 826
AclWI GGATC 2 cut(s) 782, 878
AcuI CTGAAG 3 cut(s) 444, 474, 744
AfaI GTAC 2 cut(s) 299, 527
AfiI CCNNNNNNNGG 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 2 cut(s) 515, 691
AhdI GACNNNNNGTC 1 cut(s) 702
AjnI CCWGG 2 cut(s) 15, 530
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 2 cut(s) 616, 740
AluI AGCT 2 cut(s) 616, 740
Alw26I GTCTC 3 cut(s) 321, 450, 671
AlwI GGATC 2 cut(s) 782, 878
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 3 cut(s) 216, 351, 501
ApeKI GCWGC 4 cut(s) 12, 149, 737, 854
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AspS9I GGNCC 4 cut(s) 19, 216, 351, 501
AsuC2I CCSGG 1 cut(s) 805
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 1 cut(s) 19
BbvI GCAGC 4 cut(s) 24, 161, 749, 866
BccI CCATC 1 cut(s) 471
BceAI ACGGC 1 cut(s) 787
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BciT130I CCWGG 2 cut(s) 17, 532
BcnI CCSGG 1 cut(s) 805
BcoDI GTCTC 3 cut(s) 321, 450, 671
BfaI CTAG 2 cut(s) 132, 303
BfmI CTRYAG 1 cut(s) 682
BfuAI ACCTGC 1 cut(s) 689
BisI GCNGC 5 cut(s) 13, 147, 150, 738, 855
BlsI GCNGC 5 cut(s) 14, 148, 151, 739, 856
Bme1390I CCNGG 3 cut(s) 17, 532, 805
Bme18I GGWCC 1 cut(s) 19
BmeRI GACNNNNNGTC 1 cut(s) 702
BmgT120I GGNCC 4 cut(s) 19, 216, 351, 501
BmiI GGNNCC 2 cut(s) 352, 502
BmrFI CCNGG 3 cut(s) 17, 532, 805
BmsI GCATC 4 cut(s) 133, 145, 613, 728
BpuEI CTTGAG 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 805
BsaI GGTCTC 1 cut(s) 671
BsaWI WCCGGW 4 cut(s) 21, 392, 846, 867
BsaXI ACNNNNNCTCC 2 cut(s) 785, 815
Bsc4I CCNNNNNNNGG 1 cut(s) 556
Bse1I ACTGG 2 cut(s) 248, 527
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 2 cut(s) 17, 532
BseGI GGATG 2 cut(s) 459, 616
BseLI CCNNNNNNNGG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 140, 789
BseNI ACTGG 2 cut(s) 248, 527
BseXI GCAGC 4 cut(s) 24, 161, 749, 866
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 218, 353, 503
BsiEI CGRYCG 1 cut(s) 223
BsiSI CCGG 5 cut(s) 22, 393, 805, 847, 868
BslFI GGGAC 2 cut(s) 214, 320
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 3 cut(s) 321, 450, 671
BsmFI GGGAC 2 cut(s) 214, 320
BsnI GGCC 3 cut(s) 218, 353, 503
Bso31I GGTCTC 1 cut(s) 671
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 3 cut(s) 220, 787, 870
BspACI CCGC 2 cut(s) 146, 826
BspANI GGCC 3 cut(s) 218, 353, 503
BspCNI CTCAG 2 cut(s) 139, 788
BspEI TCCGGA 1 cut(s) 21
BspLI GGNNCC 2 cut(s) 352, 502
BspMAI CTGCAG 1 cut(s) 686
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 2 cut(s) 782, 878
BspTNI GGTCTC 1 cut(s) 671
BsrI ACTGG 2 cut(s) 248, 527
BssMI GATC 3 cut(s) 220, 787, 870
Bst2UI CCWGG 2 cut(s) 17, 532
Bst4CI ACNGT 3 cut(s) 112, 205, 432
Bst6I CTCTTC 1 cut(s) 579
BstAPI GCANNNNNTGC 1 cut(s) 155
BstDEI CTNAG 2 cut(s) 126, 775
BstEII GGTNACC 1 cut(s) 807
BstF5I GGATG 2 cut(s) 459, 616
BstKTI GATC 3 cut(s) 223, 790, 873
BstMAI GTCTC 3 cut(s) 321, 450, 671
BstMBI GATC 3 cut(s) 220, 787, 870
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 2 cut(s) 17, 532
BstNSI RCATGY 2 cut(s) 479, 705
BstPI GGTNACC 1 cut(s) 807
BstSCI CCNGG 3 cut(s) 15, 530, 803
BstSFI CTRYAG 1 cut(s) 682
BstV1I GCAGC 4 cut(s) 24, 161, 749, 866
BstX2I RGATCY 2 cut(s) 787, 870
BstYI RGATCY 2 cut(s) 787, 870
BsuRI GGCC 3 cut(s) 218, 353, 503
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 459, 616
BtsI GCAGTG 2 cut(s) 41, 165
BtsIMutI CAGTG 6 cut(s) 41, 165, 241, 420, 428, 520
BveI ACCTGC 1 cut(s) 689
Cfr13I GGNCC 4 cut(s) 19, 216, 351, 501
Csp6I GTAC 2 cut(s) 298, 526
CviAII CATG 3 cut(s) 83, 476, 702
CviQI GTAC 2 cut(s) 298, 526
DdeI CTNAG 2 cut(s) 126, 775
DpnI GATC 3 cut(s) 222, 789, 872
DpnII GATC 3 cut(s) 220, 787, 870
DrdI GACNNNNNNGTC 1 cut(s) 776
DriI GACNNNNNGTC 1 cut(s) 702
DseDI GACNNNNNNGTC 1 cut(s) 776
Eam1104I CTCTTC 1 cut(s) 579
Eam1105I GACNNNNNGTC 1 cut(s) 702
EarI CTCTTC 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 3 cut(s) 444, 474, 744
Eco91I GGTNACC 1 cut(s) 807
EcoO65I GGTNACC 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 15, 530
EcoT22I ATGCAT 1 cut(s) 721
FaeI CATG 3 cut(s) 86, 479, 705
FaqI GGGAC 2 cut(s) 214, 320
FatI CATG 3 cut(s) 82, 475, 701
FauNDI CATATG 1 cut(s) 212
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 5 cut(s) 13, 147, 150, 738, 855
FokI GGATG 2 cut(s) 446, 623
Fsp4HI GCNGC 5 cut(s) 13, 147, 150, 738, 855
FspBI CTAG 2 cut(s) 132, 303
GluI GCNGC 5 cut(s) 13, 147, 150, 738, 855
HaeIII GGCC 3 cut(s) 218, 353, 503
HapII CCGG 5 cut(s) 22, 393, 805, 847, 868
Hin1II CATG 3 cut(s) 86, 479, 705
HinfI GANTC 2 cut(s) 79, 843
HpaII CCGG 5 cut(s) 22, 393, 805, 847, 868
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 5 cut(s) 41, 300, 520, 528, 766
Hpy188I TCNGA 4 cut(s) 409, 455, 493, 724
Hpy188III TCNNGA 4 cut(s) 22, 275, 608, 707
Hpy8I GTNNAC 5 cut(s) 41, 300, 520, 528, 766
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 2 cut(s) 266, 719
HpyCH4III ACNGT 3 cut(s) 112, 205, 432
HpyCH4V TGCA 8 cut(s) 158, 185, 210, 288, 312, 347, 684, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 126, 775
Hsp92II CATG 3 cut(s) 86, 479, 705
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 3 cut(s) 220, 787, 870
Lsp1109I GCAGC 4 cut(s) 24, 161, 749, 866
LweI GCATC 4 cut(s) 133, 145, 613, 728
MaeI CTAG 2 cut(s) 132, 303
MaeIII GTNAC 2 cut(s) 101, 807
MalI GATC 3 cut(s) 222, 789, 872
MboI GATC 3 cut(s) 220, 787, 870
MfeI CAATTG 1 cut(s) 167
MflI RGATCY 2 cut(s) 787, 870
MluCI AATT 3 cut(s) 120, 167, 361
MlyI GAGTC 2 cut(s) 88, 852
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 5 cut(s) 91, 428, 480, 582, 809
Mph1103I ATGCAT 1 cut(s) 721
MroI TCCGGA 1 cut(s) 21
MseI TTAA 2 cut(s) 630, 666
MslI CAYNNNNRTG 1 cut(s) 110
MspI CCGG 5 cut(s) 22, 393, 805, 847, 868
MspR9I CCNGG 3 cut(s) 17, 532, 805
MunI CAATTG 1 cut(s) 167
MvaI CCWGG 2 cut(s) 17, 532
MwoI GCNNNNNNNGC 1 cut(s) 155
NciI CCSGG 1 cut(s) 805
NdeI CATATG 1 cut(s) 212
NdeII GATC 3 cut(s) 220, 787, 870
NlaIII CATG 3 cut(s) 86, 479, 705
NlaIV GGNNCC 2 cut(s) 352, 502
NmuCI GTSAC 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 2 cut(s) 479, 705
OliI CACNNNNGTG 1 cut(s) 110
PciI ACATGT 1 cut(s) 475
PkrI GCNGC 5 cut(s) 14, 148, 151, 739, 856
Ple19I CGATCG 1 cut(s) 223
PleI GAGTC 2 cut(s) 87, 851
PpsI GAGTC 2 cut(s) 87, 851
PscI ACATGT 1 cut(s) 475
Psp6I CCWGG 2 cut(s) 15, 530
PspEI GGTNACC 1 cut(s) 807
PspGI CCWGG 2 cut(s) 15, 530
PspN4I GGNNCC 2 cut(s) 352, 502
PspPI GGNCC 4 cut(s) 19, 216, 351, 501
PstI CTGCAG 1 cut(s) 686
PsuI RGATCY 2 cut(s) 787, 870
PvuI CGATCG 1 cut(s) 223
RsaI GTAC 2 cut(s) 299, 527
RsaNI GTAC 2 cut(s) 298, 526
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 2 cut(s) 630, 666
SatI GCNGC 5 cut(s) 13, 147, 150, 738, 855
Sau3AI GATC 3 cut(s) 220, 787, 870
Sau96I GGNCC 4 cut(s) 19, 216, 351, 501
SchI GAGTC 2 cut(s) 88, 852
ScrFI CCNGG 3 cut(s) 17, 532, 805
SfaNI GCATC 4 cut(s) 133, 145, 613, 728
SfcI CTRYAG 1 cut(s) 682
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
Sse9I AATT 3 cut(s) 120, 167, 361
SsiI CCGC 2 cut(s) 146, 826
SspMI CTAG 2 cut(s) 132, 303
StyD4I CCNGG 3 cut(s) 15, 530, 803
TaaI ACNGT 3 cut(s) 112, 205, 432
TaqI TCGA 2 cut(s) 96, 573
TasI AATT 3 cut(s) 120, 167, 361
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 149
Tru1I TTAA 2 cut(s) 630, 666
Tru9I TTAA 2 cut(s) 630, 666
TscAI CASTG 6 cut(s) 48, 165, 248, 427, 435, 527
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 4 cut(s) 12, 149, 737, 854
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 675
TspRI CASTG 6 cut(s) 48, 165, 248, 427, 435, 527
VpaK11BI GGWCC 1 cut(s) 19
XceI RCATGY 2 cut(s) 479, 705
XmiI GTMKAC 1 cut(s) 765
XspI CTAG 2 cut(s) 132, 303
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.