MD13G1043300.v1.1

transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011497
Physical Location & Seq
Forward (+)
3035898 .. 3037248
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1043300.v1.1.491

Sequence Viewer

Length: 702 bp
ATGACTTTAACGGCGAAGCACGGCGGCGGAACCAGAGCCATGGACGGTATCGCAATGGCGGAGTCGACGTCGACGCCTCCTCTAACGGAAATCGTCGAGGCCTTCGAGGAGTTGGGCAAGATCCTCCAATCCCATTCCCATAATTCCCAACGAAAACAACACCAAACGTTGCGTTTGGATACATTCTCCCAGGCTTCCACCCTCGTCTCCATCCTCTTCAGTTGCCTTGGCCTCGCCTTCAAGTTCGCCGAGATGGAGTACGTCTCCAAGGTTAAAGATCTTGTGGAGGCGTCACGGACTTACGACACATTAGAAAGCCTTATAGATTATGATGTTGCTAAGGGCACGGTGAAATCCCCCGGAAGCCATTCCCGTAATCTGCGCCGTGTCCGGCAGGGTCTCGACCTCATCAGAGCTCTATTCGAACAATTCTTGTCAACCGATGACTACTCTTTGAGGGAAGCAGCTTCAACAGCTTATGCCAAAGTGTGTGCGCCATACCACAGTTGGGCAATCAGGACAGCGGTTGCTGCCGGAATGTATGCTCTTCCGTCGAGGGATCAACTGCTGGTGAATCTCGAAGAGACCCGTAAGTCTAAAAGCTTTTCTCCGTCCAGAAACAATGATCTCCAGTTTTGCATGCTGTTTATTTCTAAAATGCAATCAATTTCATTCCAGATCAGTCAGCAGAGAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

234

Amino Acids

26.14

Weight (kDa)

9.13

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 58 - 193 2.4e-36 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 592
AatII GACGTC 1 cut(s) 71
AccI GTMKAC 2 cut(s) 65, 71
AciI CCGC 4 cut(s) 24, 27, 59, 524
AclI AACGTT 1 cut(s) 167
AclWI GGATC 2 cut(s) 115, 567
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 3 cut(s) 68, 74, 290
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 508
AgsI TTSAA 2 cut(s) 241, 471
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 4 cut(s) 416, 467, 476, 603
AluI AGCT 4 cut(s) 416, 467, 476, 603
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 4 cut(s) 211, 268, 404, 578
AlwI GGATC 2 cut(s) 115, 567
AoxI GGCC 2 cut(s) 99, 229
ApeKI GCWGC 2 cut(s) 464, 530
Asp700I GAANNNNTTC 1 cut(s) 367
AspLEI GCGC 2 cut(s) 384, 496
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 361, 583
AsuII TTCGAA 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 476, 517
BccI CCATC 2 cut(s) 218, 247
BceAI ACGGC 3 cut(s) 27, 37, 369
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 172
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 4 cut(s) 211, 268, 404, 578
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 277
BisI GCNGC 3 cut(s) 25, 465, 531
BlsI GCNGC 3 cut(s) 26, 466, 532
Bme1390I CCNGG 2 cut(s) 191, 360
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 191, 360
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 360
BsaHI GRCGYC 3 cut(s) 68, 74, 290
BsaI GGTCTC 2 cut(s) 404, 578
BsaJI CCNNGG 5 cut(s) 39, 189, 226, 267, 358
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 5 cut(s) 39, 189, 226, 267, 358
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMI GCAATG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 2 cut(s) 69, 122
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 2 cut(s) 476, 517
BshFI GGCC 2 cut(s) 101, 231
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 3 cut(s) 360, 391, 534
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 4 cut(s) 211, 268, 404, 578
BsmBI CGTCTC 2 cut(s) 211, 268
BsnI GGCC 2 cut(s) 101, 231
Bso31I GGTCTC 2 cut(s) 404, 578
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 347, 418
Bsp143I GATC 5 cut(s) 120, 277, 559, 625, 678
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 4 cut(s) 24, 27, 59, 524
BspANI GGCC 2 cut(s) 101, 231
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 2 cut(s) 115, 567
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 423
BspTNI GGTCTC 2 cut(s) 404, 578
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 5 cut(s) 39, 189, 226, 267, 358
BssMI GATC 5 cut(s) 120, 277, 559, 625, 678
BssNI GRCGYC 3 cut(s) 68, 74, 290
BssT1I CCWWGG 3 cut(s) 39, 226, 267
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 47, 349, 506
Bst6I CTCTTC 3 cut(s) 221, 552, 576
BstACI GRCGYC 3 cut(s) 68, 74, 290
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 210
BstHHI GCGC 2 cut(s) 384, 496
BstKTI GATC 5 cut(s) 123, 280, 562, 628, 681
BstMAI GTCTC 4 cut(s) 211, 268, 404, 578
BstMBI GATC 5 cut(s) 120, 277, 559, 625, 678
BstMWI GCNNNNNNNGC 2 cut(s) 473, 530
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 2 cut(s) 189, 358
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 476, 517
BstX2I RGATCY 2 cut(s) 120, 277
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 2 cut(s) 120, 277
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 2 cut(s) 101, 231
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 2 cut(s) 384, 496
CseI GACGC 2 cut(s) 82, 279
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 40, 640
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 339
DpnI GATC 5 cut(s) 122, 279, 561, 627, 680
DpnII GATC 5 cut(s) 120, 277, 559, 625, 678
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
Eam1104I CTCTTC 3 cut(s) 221, 552, 576
EarI CTCTTC 3 cut(s) 221, 552, 576
EciI GGCGGA 2 cut(s) 42, 74
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 3 cut(s) 39, 226, 267
Eco147I AGGCCT 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 2 cut(s) 404, 578
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 3 cut(s) 39, 226, 267
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 39, 226, 267
Esp3I CGTCTC 2 cut(s) 211, 268
FaeI CATG 2 cut(s) 43, 643
FaiI YATR 8 cut(s) 41, 141, 323, 330, 480, 499, 543, 641
FatI CATG 2 cut(s) 39, 639
FblI GTMKAC 2 cut(s) 65, 71
Fnu4HI GCNGC 3 cut(s) 25, 465, 531
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 3 cut(s) 25, 465, 531
GlaI GCGC 2 cut(s) 383, 495
GluI GCNGC 3 cut(s) 25, 465, 531
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 101, 231
HapII CCGG 3 cut(s) 360, 391, 534
HgaI GACGC 2 cut(s) 82, 279
HhaI GCGC 2 cut(s) 384, 496
Hin1I GRCGYC 3 cut(s) 68, 74, 290
Hin1II CATG 2 cut(s) 43, 643
Hin6I GCGC 2 cut(s) 382, 494
HinP1I GCGC 2 cut(s) 382, 494
HincII GTYRAC 3 cut(s) 66, 72, 438
HindII GTYRAC 3 cut(s) 66, 72, 438
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 2 cut(s) 62, 574
HpaII CCGG 3 cut(s) 360, 391, 534
HphI GGTGA 2 cut(s) 361, 583
Hpy166II GTNNAC 3 cut(s) 66, 72, 438
Hpy188I TCNGA 1 cut(s) 413
Hpy188III TCNNGA 5 cut(s) 401, 517, 578, 615, 676
Hpy8I GTNNAC 3 cut(s) 66, 72, 438
Hpy99I CGWCG 5 cut(s) 70, 73, 76, 98, 556
HpyAV CCTTC 2 cut(s) 112, 247
HpyCH4III ACNGT 3 cut(s) 47, 349, 506
HpyCH4IV ACGT 3 cut(s) 68, 167, 261
HpyCH4V TGCA 2 cut(s) 639, 661
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 530
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 3 cut(s) 68, 167, 261
Hsp92I GRCGYC 3 cut(s) 68, 74, 290
Hsp92II CATG 2 cut(s) 43, 643
HspAI GCGC 2 cut(s) 382, 494
Kzo9I GATC 5 cut(s) 120, 277, 559, 625, 678
LguI GCTCTTC 1 cut(s) 552
Lsp1109I GCAGC 2 cut(s) 476, 517
MaeII ACGT 3 cut(s) 68, 167, 261
MaeIII GTNAC 1 cut(s) 291
MalI GATC 5 cut(s) 122, 279, 561, 627, 680
MboI GATC 5 cut(s) 120, 277, 559, 625, 678
MboII GAAGA 3 cut(s) 208, 539, 593
MflI RGATCY 2 cut(s) 120, 277
MhlI GDGCHC 2 cut(s) 347, 418
MluCI AATT 3 cut(s) 142, 428, 666
MlyI GAGTC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 2 cut(s) 8, 273
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 3 cut(s) 360, 391, 534
MspR9I CCNGG 2 cut(s) 191, 360
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 473, 530
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 1 cut(s) 39
NdeII GATC 5 cut(s) 120, 277, 559, 625, 678
NlaIII CATG 2 cut(s) 43, 643
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 1 cut(s) 291
NspI RCATGY 1 cut(s) 643
NspV TTCGAA 1 cut(s) 423
PaeI GCATGC 1 cut(s) 643
PceI AGGCCT 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 1 cut(s) 574
PkrI GCNGC 3 cut(s) 26, 466, 532
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 31
PsuI RGATCY 2 cut(s) 120, 277
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SacI GAGCTC 1 cut(s) 418
SalI GTCGAC 2 cut(s) 64, 70
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 8, 273
SatI GCNGC 3 cut(s) 25, 465, 531
Sau3AI GATC 5 cut(s) 120, 277, 559, 625, 678
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 191, 360
SduI GDGCHC 2 cut(s) 347, 418
SetI ASST 9 cut(s) 71, 170, 264, 273, 408, 418, 469, 478, 605
SfuI TTCGAA 1 cut(s) 423
SgrDI CGTCGACG 1 cut(s) 70
SphI GCATGC 1 cut(s) 643
Sse9I AATT 3 cut(s) 142, 428, 666
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 4 cut(s) 24, 27, 59, 524
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 189, 358
StyI CCWWGG 3 cut(s) 39, 226, 267
TaaI ACNGT 3 cut(s) 47, 349, 506
TaiI ACGT 3 cut(s) 71, 170, 264
TaqI TCGA 8 cut(s) 65, 71, 96, 105, 402, 423, 554, 579
TasI AATT 3 cut(s) 142, 428, 666
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 2 cut(s) 8, 273
Tru9I TTAA 2 cut(s) 8, 273
TseFI GTSAC 1 cut(s) 291
TseI GCWGC 2 cut(s) 464, 530
Tsp45I GTSAC 1 cut(s) 291
TspDTI ATGAA 1 cut(s) 660
TspGWI ACGGA 4 cut(s) 101, 310, 540, 600
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XmiI GTMKAC 2 cut(s) 65, 71
XmnI GAANNNNTTC 1 cut(s) 367
ZraI GACGTC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.