Rorug04G0284800

transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011497
Physical Location & Seq
Reverse (-)
45792649 .. 45796107
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0284800.1

Sequence Viewer

Length: 1035 bp
ATGATGTTCAAGTTACAAGGTTTAGTTGCCTTCGCAATCACATTGTTCGTCTCCGCCTCTTTCACTCATGGCGGCCACCCCAGCGTCGAAGATGAAAAGATCGGCTCTCCCCTCCTGACTCAAGAGCTACACACCAACAACACCATCCTGGTCGATATCAACGGCGGTGGCCAATTCAAGTCCATCCAGGCCGCCATCGATTCTGTTCCTGCAGGCAACAATAAGTGGGTTATCATCCATGTTAGGAGAGGGGTGTACAGAGAAAAAGTGCACGTACCCCAAGACAAACCCCATATTTTCTTGAGAGGCAATGGGAGGGGAAAGACACACATTGTTTGGTCCCAAAGCTCTCATGACAACATTGAATCTGCAACTTTCAAAGTAGAATCTCCATACTTCATTGCCTTTGGAATCAGCTTCAAGAATGAGGCTCCAACTGGTGTGGCATACACGTCGCAAAATCAGTCGGTGGCAGCATTTGTGGGTGCAGACAGGGTTGCATTTTACCACTGTGCTTTCTACAGTACCCATAACACCCTTTTCGATTACAAGGGCAGGCATTACTATGATAACTGCTACATCCAAGGCTCAATCGACTTCATCTTCGGACGTGCTAGGTCTACCTTCCATAGCTGTGAGATTTTTGTGATTTCAGACAAGAGGGTTTCCATCAAAGGGTCTGTAACAGCTAATCACCGAGAGAATGCGAACGAGTTCAGTGGGTTTGTATTTATCAAAGGCAAGGTGTATGGTGTAGGTGATCATGTCTACTTGGGTAGAGCCAAGGGCCCCTTTTCCAGAGTCGTTTTTGCAGAAACCTACCTCTCTAAGACCATTGTGCCCCAGGGTTGGACCAATTGGAGTTATGCCGGAGGAACAGAGAACCTGTTCCACGCGGAGTACAATTGCAAAGGGCCAGGAGCCGACTTTGCACAGCGCGCTCCATGGGCGAAGCAACTGACGGAGAAAGAGGCCGCACCATTCCTGTCGATCGACTTCATCGACGGACGGGAATGGCTCCCAGTGTGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

344

Amino Acids

38.44

Weight (kDa)

8.72

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 49 - 336 5.6e-65 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 620, 768
AccII CGCG 2 cut(s) 896, 939
AciI CCGC 6 cut(s) 54, 72, 165, 192, 896, 975
AcoI YGGCCR 2 cut(s) 73, 169
AfaI GTAC 4 cut(s) 257, 276, 526, 902
AfiI CCNNNNNNNGG 2 cut(s) 675, 849
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 5 cut(s) 10, 178, 365, 379, 421
AjiI CACGTC 2 cut(s) 453, 611
AjnI CCWGG 4 cut(s) 147, 186, 843, 916
AluBI AGCT 5 cut(s) 127, 348, 417, 633, 689
AluI AGCT 5 cut(s) 127, 348, 417, 633, 689
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 55
Alw44I GTGCAC 1 cut(s) 269
AoxI GGCC 6 cut(s) 73, 169, 189, 787, 914, 972
ApaI GGGCCC 1 cut(s) 791
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 473
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
Asp700I GAANNNNTTC 2 cut(s) 713, 887
AspLEI GCGC 2 cut(s) 939, 941
AspS9I GGNCC 5 cut(s) 339, 787, 788, 852, 914
AsuHPI GGTGA 2 cut(s) 686, 770
AvaII GGWCC 2 cut(s) 339, 852
BaeGI GKGCMC 3 cut(s) 273, 791, 843
BalI TGGCCA 1 cut(s) 171
BanII GRGCYC 1 cut(s) 791
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 485
BccI CCATC 4 cut(s) 152, 191, 203, 677
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciT130I CCWGG 4 cut(s) 149, 188, 845, 918
BclI TGATCA 1 cut(s) 760
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 2 cut(s) 210, 520
BisI GCNGC 4 cut(s) 73, 192, 474, 975
BlsI GCNGC 4 cut(s) 74, 193, 475, 976
Bme1390I CCNGG 4 cut(s) 149, 188, 845, 918
Bme18I GGWCC 2 cut(s) 339, 852
BmgBI CACGTC 2 cut(s) 453, 611
BmgT120I GGNCC 5 cut(s) 339, 787, 788, 852, 914
BmiI GGNNCC 6 cut(s) 341, 432, 789, 790, 922, 1019
BmrFI CCNGG 4 cut(s) 149, 188, 845, 918
BmrI ACTGGG 1 cut(s) 1016
BmuI ACTGGG 1 cut(s) 1016
BpuEI CTTGAG 2 cut(s) 105, 322
Bsa29I ATCGAT 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 274
BsaJI CCNNGG 5 cut(s) 583, 783, 843, 844, 944
Bsc4I CCNNNNNNNGG 2 cut(s) 675, 849
Bse1I ACTGG 2 cut(s) 442, 1022
Bse3DI GCAATG 2 cut(s) 316, 399
BseBI CCWGG 4 cut(s) 149, 188, 845, 918
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 5 cut(s) 583, 783, 843, 844, 944
BseGI GGATG 4 cut(s) 144, 183, 234, 579
BseLI CCNNNNNNNGG 2 cut(s) 675, 849
BseMI GCAATG 2 cut(s) 316, 399
BseNI ACTGG 2 cut(s) 442, 1022
BsePI GCGCGC 1 cut(s) 937
BseSI GKGCMC 3 cut(s) 273, 791, 843
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 896, 939
Bsh1285I CGRYCG 1 cut(s) 993
BshFI GGCC 6 cut(s) 75, 171, 191, 789, 916, 974
BshVI ATCGAT 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 993
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 870
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 675, 849
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 6 cut(s) 75, 171, 191, 789, 916, 974
Bsp120I GGGCCC 1 cut(s) 787
Bsp1286I GDGCHC 3 cut(s) 273, 791, 843
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 3 cut(s) 99, 760, 990
Bsp19I CCATGG 1 cut(s) 944
BspACI CCGC 6 cut(s) 54, 72, 165, 192, 896, 975
BspANI GGCC 6 cut(s) 75, 171, 191, 789, 916, 974
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 2 cut(s) 896, 939
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 6 cut(s) 341, 432, 789, 790, 922, 1019
BspMAI CTGCAG 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 316, 399
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 2 cut(s) 442, 1022
BssECI CCNNGG 5 cut(s) 583, 783, 843, 844, 944
BssHII GCGCGC 1 cut(s) 937
BssMI GATC 3 cut(s) 99, 760, 990
BssT1I CCWWGG 3 cut(s) 583, 783, 944
Bst2UI CCWGG 4 cut(s) 149, 188, 845, 918
Bst4CI ACNGT 2 cut(s) 512, 524
BstAUI TGTACA 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 274
BstC8I GCNNGC 3 cut(s) 214, 557, 939
BstDEI CTNAG 1 cut(s) 828
BstDSI CCRYGG 1 cut(s) 944
BstF5I GGATG 4 cut(s) 144, 183, 234, 579
BstFNI CGCG 2 cut(s) 896, 939
BstHHI GCGC 2 cut(s) 939, 941
BstKTI GATC 3 cut(s) 102, 763, 993
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 99, 760, 990
BstMCI CGRYCG 1 cut(s) 993
BstMWI GCNNNNNNNGC 4 cut(s) 81, 929, 938, 947
BstNI CCWGG 4 cut(s) 149, 188, 845, 918
BstSCI CCNGG 4 cut(s) 147, 186, 843, 916
BstSFI CTRYAG 2 cut(s) 210, 520
BstSLI GKGCMC 3 cut(s) 273, 791, 843
BstUI CGCG 2 cut(s) 896, 939
BstV1I GCAGC 1 cut(s) 485
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 6 cut(s) 75, 171, 191, 789, 916, 974
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 944
BtrI CACGTC 2 cut(s) 453, 611
BtsCI GGATG 4 cut(s) 144, 183, 234, 579
BtsIMutI CAGTG 3 cut(s) 508, 724, 1029
Cac8I GCNNGC 3 cut(s) 214, 557, 939
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 939, 941
Cfr13I GGNCC 5 cut(s) 339, 787, 788, 852, 914
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 256, 275, 525, 901
CspCI CAANNNNNGTGG 4 cut(s) 148, 183, 423, 458
CviAII CATG 5 cut(s) 68, 239, 353, 764, 945
CviQI GTAC 4 cut(s) 256, 275, 525, 901
DdeI CTNAG 1 cut(s) 828
DpnI GATC 3 cut(s) 101, 762, 992
DpnII GATC 3 cut(s) 99, 760, 990
EaeI YGGCCR 2 cut(s) 73, 169
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 3 cut(s) 583, 783, 944
Eco24I GRGCYC 1 cut(s) 791
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 339, 852
EcoO109I RGGNCCY 2 cut(s) 787, 788
EcoRII CCWGG 4 cut(s) 147, 186, 843, 916
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 3 cut(s) 583, 783, 944
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 3 cut(s) 583, 783, 944
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 71, 242, 356, 767, 948
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 1 cut(s) 325
FatI CATG 5 cut(s) 67, 238, 352, 763, 944
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 2 cut(s) 620, 768
Fnu4HI GCNGC 4 cut(s) 73, 192, 474, 975
FokI GGATG 4 cut(s) 131, 170, 221, 566
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 4 cut(s) 73, 192, 474, 975
FspBI CTAG 1 cut(s) 615
GlaI GCGC 2 cut(s) 938, 940
GluI GCNGC 4 cut(s) 73, 192, 474, 975
GsaI CCCAGC 1 cut(s) 84
HaeIII GGCC 6 cut(s) 75, 171, 191, 789, 916, 974
HapII CCGG 1 cut(s) 870
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 939, 941
Hin1II CATG 5 cut(s) 71, 242, 356, 767, 948
Hin6I GCGC 2 cut(s) 937, 939
HinP1I GCGC 2 cut(s) 937, 939
HinfI GANTC 6 cut(s) 118, 200, 365, 386, 411, 801
HpaII CCGG 1 cut(s) 870
HphI GGTGA 2 cut(s) 686, 770
Hpy166II GTNNAC 4 cut(s) 256, 271, 621, 769
Hpy188I TCNGA 2 cut(s) 608, 655
Hpy188III TCNNGA 6 cut(s) 115, 122, 301, 353, 421, 798
Hpy8I GTNNAC 4 cut(s) 256, 271, 621, 769
Hpy99I CGWCG 3 cut(s) 89, 457, 1007
HpyAV CCTTC 2 cut(s) 40, 634
HpyCH4III ACNGT 2 cut(s) 512, 524
HpyCH4IV ACGT 3 cut(s) 273, 452, 610
HpyCH4V TGCA 8 cut(s) 212, 271, 371, 488, 500, 812, 909, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 929, 938, 947
HpyF3I CTNAG 1 cut(s) 828
HpySE526I ACGT 3 cut(s) 273, 452, 610
Hsp92II CATG 5 cut(s) 71, 242, 356, 767, 948
HspAI GCGC 2 cut(s) 937, 939
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 3 cut(s) 99, 760, 990
LmnI GCTCC 4 cut(s) 436, 920, 946, 1023
Lsp1109I GCAGC 1 cut(s) 485
MaeI CTAG 1 cut(s) 615
MaeII ACGT 3 cut(s) 273, 452, 610
MaeIII GTNAC 2 cut(s) 12, 682
MalI GATC 3 cut(s) 101, 762, 992
MboI GATC 3 cut(s) 99, 760, 990
MboII GAAGA 2 cut(s) 101, 595
MfeI CAATTG 2 cut(s) 856, 904
MhlI GDGCHC 3 cut(s) 273, 791, 843
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 3 cut(s) 173, 856, 904
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 2 cut(s) 112, 810
MmeI TCCRAC 2 cut(s) 458, 830
Mox20I TGGCCA 1 cut(s) 171
MroXI GAANNNNTTC 2 cut(s) 713, 887
MscI TGGCCA 1 cut(s) 171
MslI CAYNNNNRTG 2 cut(s) 564, 633
Msp20I TGGCCA 1 cut(s) 171
MspI CCGG 1 cut(s) 870
MspR9I CCNGG 4 cut(s) 149, 188, 845, 918
MunI CAATTG 2 cut(s) 856, 904
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 4 cut(s) 149, 188, 845, 918
MvnI CGCG 2 cut(s) 896, 939
MwoI GCNNNNNNNGC 4 cut(s) 81, 929, 938, 947
NcoI CCATGG 1 cut(s) 944
NdeII GATC 3 cut(s) 99, 760, 990
NlaIII CATG 5 cut(s) 71, 242, 356, 767, 948
NlaIV GGNNCC 6 cut(s) 341, 432, 789, 790, 922, 1019
PagI TCATGA 1 cut(s) 352
PasI CCCWGGG 1 cut(s) 844
PauI GCGCGC 1 cut(s) 937
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 2 cut(s) 713, 887
PfeI GAWTC 4 cut(s) 200, 365, 386, 411
PkrI GCNGC 4 cut(s) 74, 193, 475, 976
Ple19I CGATCG 1 cut(s) 993
PleI GAGTC 2 cut(s) 112, 809
PpsI GAGTC 2 cut(s) 112, 809
Ppu21I YACGTR 1 cut(s) 274
Psp6I CCWGG 4 cut(s) 147, 186, 843, 916
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 4 cut(s) 147, 186, 843, 916
PspN4I GGNNCC 6 cut(s) 341, 432, 789, 790, 922, 1019
PspOMI GGGCCC 1 cut(s) 787
PspPI GGNCC 5 cut(s) 339, 787, 788, 852, 914
PstI CTGCAG 1 cut(s) 214
PteI GCGCGC 1 cut(s) 937
PvuI CGATCG 1 cut(s) 993
RsaI GTAC 4 cut(s) 257, 276, 526, 902
RsaNI GTAC 4 cut(s) 256, 275, 525, 901
RseI CAYNNNNRTG 2 cut(s) 564, 633
SatI GCNGC 4 cut(s) 73, 192, 474, 975
Sau3AI GATC 3 cut(s) 99, 760, 990
Sau96I GGNCC 5 cut(s) 339, 787, 788, 852, 914
SbfI CCTGCAGG 1 cut(s) 214
SchI GAGTC 2 cut(s) 112, 810
ScrFI CCNGG 4 cut(s) 149, 188, 845, 918
SdaI CCTGCAGG 1 cut(s) 214
SduI GDGCHC 3 cut(s) 273, 791, 843
SfcI CTRYAG 2 cut(s) 210, 520
SinI GGWCC 2 cut(s) 339, 852
SmiMI CAYNNNNRTG 2 cut(s) 564, 633
SmlI CTYRAG 2 cut(s) 120, 301
SmoI CTYRAG 2 cut(s) 120, 301
Sse8387I CCTGCAGG 1 cut(s) 214
Sse9I AATT 3 cut(s) 173, 856, 904
SsiI CCGC 6 cut(s) 54, 72, 165, 192, 896, 975
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 4 cut(s) 147, 186, 843, 916
StyI CCWWGG 3 cut(s) 583, 783, 944
TaaI ACNGT 2 cut(s) 512, 524
TaiI ACGT 3 cut(s) 276, 455, 613
TaqI TCGA 8 cut(s) 87, 153, 198, 543, 594, 989, 993, 1002
TasI AATT 3 cut(s) 173, 856, 904
TatI WGTACW 2 cut(s) 255, 900
TauI GCSGC 3 cut(s) 75, 194, 977
TfiI GAWTC 4 cut(s) 200, 365, 386, 411
TscAI CASTG 3 cut(s) 515, 724, 1029
TseI GCWGC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 108, 388, 589, 988
TspGWI ACGGA 2 cut(s) 977, 1020
TspRI CASTG 3 cut(s) 515, 724, 1029
VneI GTGCAC 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 339, 852
XmiI GTMKAC 2 cut(s) 620, 768
XmnI GAANNNNTTC 2 cut(s) 713, 887
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.