pycom13g03810

transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011497
Physical Location & Seq
Forward (+)
2537188 .. 2538530
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03810.1

Sequence Viewer

Length: 696 bp
ATGATTTTAACGGCGAAGCACGGCGGCGGAACCAGAGCCATGGACGGTATCGCAATGGCGGAGTCGACCTCGACGCCTCCTCTAACGGAAATCGTTGAGGCCTTTGAGGAGTTGAGCAAGATCCTCCAATCCCATTCCCATAATTCCCAACAAAAACAACACCAAACGTTGCGTTTGGATACCTTTTCCCAGGCTTCCACCCTCGTCTCCATCCTCTTCAGTTGCCTTGGCCTCGCCTTCAAGTTCGCCGAGATGGAGTACGTCTCCAAGGTTAAAGATCTTGTGGAGGCGTCACGGACTTACGACACATTAGAAAGCCTTATAGATTTTGATGTTGCTAACGGCACGGTGAAATCCCCCGGAAGCCATTCTCGTAATCTGCGCCGTGTCCGGCAGGGTCTCGACCTCATCAGAGCTCTATTCGAACAATTCTTGTCAACCGATGACTACTCTTTGAGGGAAGCAGCTTCAACAGCTTATGCCAAAGTTTGTGCGCCATACCACAGTTGGGCAATCAGGACAGCGGTTGCTGCCGGAATGTATGCTCTTCCGTCGAGGGATCAACTGCTGGTGAATCTCGAAGAGACCCATCAGTCAGCAGAGAAGAAGATGAAAAGGTACATCCAAGCGTCGCGTTCGGTGATCGAGTACATCGACAGGCTATACATTTCTAAGAACATCACCTTGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

232

Amino Acids

25.93

Weight (kDa)

6.66

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 58 - 193 4.4e-36 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 65
AccII CGCG 1 cut(s) 634
AciI CCGC 4 cut(s) 24, 27, 59, 524
AclI AACGTT 1 cut(s) 167
AclWI GGATC 2 cut(s) 115, 567
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 2 cut(s) 74, 290
AfaI GTAC 3 cut(s) 260, 620, 650
AfiI CCNNNNNNNGG 2 cut(s) 508, 690
AgsI TTSAA 2 cut(s) 241, 471
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 1 cut(s) 668
AluBI AGCT 3 cut(s) 416, 467, 476
AluI AGCT 3 cut(s) 416, 467, 476
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 4 cut(s) 211, 268, 404, 578
AlwI GGATC 2 cut(s) 115, 567
AoxI GGCC 2 cut(s) 99, 229
ApeKI GCWGC 2 cut(s) 464, 530
Asp700I GAANNNNTTC 1 cut(s) 367
AspLEI GCGC 2 cut(s) 384, 496
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 4 cut(s) 361, 583, 652, 673
AsuII TTCGAA 1 cut(s) 423
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 476, 517
BccI CCATC 3 cut(s) 218, 247, 597
BceAI ACGGC 4 cut(s) 27, 37, 358, 369
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 172
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 4 cut(s) 211, 268, 404, 578
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 277
BisI GCNGC 3 cut(s) 25, 465, 531
BlsI GCNGC 3 cut(s) 26, 466, 532
Bme1390I CCNGG 2 cut(s) 191, 360
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 191, 360
BplI GAGNNNNNCTC 4 cut(s) 53, 85, 248, 280
Bpu14I TTCGAA 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 74, 290
BsaI GGTCTC 2 cut(s) 404, 578
BsaJI CCNNGG 6 cut(s) 39, 189, 226, 267, 358, 684
Bsc4I CCNNNNNNNGG 2 cut(s) 508, 690
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 6 cut(s) 39, 189, 226, 267, 358, 684
BseGI GGATG 2 cut(s) 210, 621
BseLI CCNNNNNNNGG 2 cut(s) 508, 690
BseMI GCAATG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 2 cut(s) 69, 122
BseXI GCAGC 2 cut(s) 476, 517
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 2 cut(s) 101, 231
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 3 cut(s) 360, 391, 534
BslI CCNNNNNNNGG 2 cut(s) 508, 690
BsmAI GTCTC 4 cut(s) 211, 268, 404, 578
BsmBI CGTCTC 2 cut(s) 211, 268
BsnI GGCC 2 cut(s) 101, 231
Bso31I GGTCTC 2 cut(s) 404, 578
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 4 cut(s) 120, 277, 559, 642
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 4 cut(s) 24, 27, 59, 524
BspANI GGCC 2 cut(s) 101, 231
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 2 cut(s) 115, 567
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 423
BspTNI GGTCTC 2 cut(s) 404, 578
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 695
BssECI CCNNGG 6 cut(s) 39, 189, 226, 267, 358, 684
BssMI GATC 4 cut(s) 120, 277, 559, 642
BssNI GRCGYC 2 cut(s) 74, 290
BssT1I CCWWGG 4 cut(s) 39, 226, 267, 684
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 47, 349, 506
Bst6I CTCTTC 3 cut(s) 221, 552, 576
BstACI GRCGYC 2 cut(s) 74, 290
BstBI TTCGAA 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 672
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 210, 621
BstFNI CGCG 1 cut(s) 634
BstHHI GCGC 2 cut(s) 384, 496
BstKTI GATC 4 cut(s) 123, 280, 562, 645
BstMAI GTCTC 4 cut(s) 211, 268, 404, 578
BstMBI GATC 4 cut(s) 120, 277, 559, 642
BstMWI GCNNNNNNNGC 2 cut(s) 473, 530
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 189, 358
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 2 cut(s) 476, 517
BstX2I RGATCY 2 cut(s) 120, 277
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 2 cut(s) 120, 277
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 2 cut(s) 101, 231
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 210, 621
CfoI GCGC 2 cut(s) 384, 496
CseI GACGC 3 cut(s) 82, 279, 618
Csp6I GTAC 3 cut(s) 259, 619, 649
CviAII CATG 1 cut(s) 40
CviQI GTAC 3 cut(s) 259, 619, 649
DdeI CTNAG 1 cut(s) 672
DpnI GATC 4 cut(s) 122, 279, 561, 644
DpnII GATC 4 cut(s) 120, 277, 559, 642
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
Eam1104I CTCTTC 3 cut(s) 221, 552, 576
EarI CTCTTC 3 cut(s) 221, 552, 576
EciI GGCGGA 2 cut(s) 42, 74
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 4 cut(s) 39, 226, 267, 684
Eco147I AGGCCT 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 2 cut(s) 404, 578
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 4 cut(s) 39, 226, 267, 684
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 4 cut(s) 39, 226, 267, 684
Esp3I CGTCTC 2 cut(s) 211, 268
FaeI CATG 1 cut(s) 43
FaiI YATR 7 cut(s) 41, 141, 323, 480, 499, 543, 664
FatI CATG 1 cut(s) 39
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 3 cut(s) 25, 465, 531
FokI GGATG 2 cut(s) 197, 608
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 3 cut(s) 25, 465, 531
GlaI GCGC 2 cut(s) 383, 495
GluI GCNGC 3 cut(s) 25, 465, 531
HaeIII GGCC 2 cut(s) 101, 231
HapII CCGG 3 cut(s) 360, 391, 534
HgaI GACGC 3 cut(s) 82, 279, 618
HhaI GCGC 2 cut(s) 384, 496
Hin1I GRCGYC 2 cut(s) 74, 290
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 2 cut(s) 382, 494
HinP1I GCGC 2 cut(s) 382, 494
HincII GTYRAC 2 cut(s) 66, 438
HindII GTYRAC 2 cut(s) 66, 438
HinfI GANTC 2 cut(s) 62, 574
HpaII CCGG 3 cut(s) 360, 391, 534
HphI GGTGA 4 cut(s) 361, 583, 652, 673
Hpy166II GTNNAC 2 cut(s) 66, 438
Hpy188I TCNGA 1 cut(s) 413
Hpy188III TCNNGA 3 cut(s) 401, 517, 578
Hpy8I GTNNAC 2 cut(s) 66, 438
Hpy99I CGWCG 3 cut(s) 76, 556, 634
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 3 cut(s) 47, 349, 506
HpyCH4IV ACGT 2 cut(s) 167, 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 530
HpyF3I CTNAG 1 cut(s) 672
HpySE526I ACGT 2 cut(s) 167, 261
Hsp92I GRCGYC 2 cut(s) 74, 290
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 2 cut(s) 382, 494
Kzo9I GATC 4 cut(s) 120, 277, 559, 642
LguI GCTCTTC 1 cut(s) 552
Lsp1109I GCAGC 2 cut(s) 476, 517
MaeII ACGT 2 cut(s) 167, 261
MaeIII GTNAC 1 cut(s) 291
MalI GATC 4 cut(s) 122, 279, 561, 644
MboI GATC 4 cut(s) 120, 277, 559, 642
MboII GAAGA 5 cut(s) 208, 539, 593, 616, 619
MflI RGATCY 2 cut(s) 120, 277
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 142, 428
MlyI GAGTC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 2 cut(s) 8, 273
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 3 cut(s) 360, 391, 534
MspR9I CCNGG 2 cut(s) 191, 360
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 2 cut(s) 473, 530
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 1 cut(s) 39
NdeII GATC 4 cut(s) 120, 277, 559, 642
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 1 cut(s) 291
NspV TTCGAA 1 cut(s) 423
PceI AGGCCT 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 1 cut(s) 574
PkrI GCNGC 3 cut(s) 26, 466, 532
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 31
PsuI RGATCY 2 cut(s) 120, 277
RsaI GTAC 3 cut(s) 260, 620, 650
RsaNI GTAC 3 cut(s) 259, 619, 649
SacI GAGCTC 1 cut(s) 418
SalI GTCGAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 8, 273
SatI GCNGC 3 cut(s) 25, 465, 531
Sau3AI GATC 4 cut(s) 120, 277, 559, 642
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 191, 360
SduI GDGCHC 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 423
Sse9I AATT 2 cut(s) 142, 428
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 4 cut(s) 24, 27, 59, 524
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 189, 358
StyI CCWWGG 4 cut(s) 39, 226, 267, 684
TaaI ACNGT 3 cut(s) 47, 349, 506
TaiI ACGT 2 cut(s) 170, 264
TaqI TCGA 8 cut(s) 65, 71, 402, 423, 554, 579, 645, 654
TasI AATT 2 cut(s) 142, 428
TatI WGTACW 1 cut(s) 648
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 2 cut(s) 8, 273
Tru9I TTAA 2 cut(s) 8, 273
TseFI GTSAC 1 cut(s) 291
TseI GCWGC 2 cut(s) 464, 530
Tsp45I GTSAC 1 cut(s) 291
TspDTI ATGAA 1 cut(s) 626
TspGWI ACGGA 3 cut(s) 101, 310, 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XmiI GTMKAC 1 cut(s) 65
XmnI GAANNNNTTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.