Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007623

Physical Location & Seq

Genomic Coordinates Forward (+)

3860555 .. 3866731

Gene Structure 6177 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1055100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGTGGAGAAACTTCGCCAAAAAGGTTGCTTCCAGAAAGGAAAATCTTTCCTCACAGTTTCATTTGGGTTCTGCTACTTCTTCTTCGGTGAAATTTTATCAAGAATCGAGCTTTCTCGAGAAAACCAGGCATTTCGAGGCCCGGAACTGCGCAAACAATAGGTTTTTGGTGATGGGTTTTCCACAAATTGGCGATAAAGCTTCCCAGAAGGACGAATTGGGTAGATGCAGTTTGATGAATCCGAGTTACTCTCATGCCGTTTCTGGGTTTTGTAGCTATGCCAGAGGCTATGCCAGTGCCGCCGAGGCCATTGCTTCGACGGATGGGGAGGAAGACTCGTCAGGGTCTGAAGAAATTCAGGAATTGTTGGAGGATTTGATCAGAGAGAACAACAAGGTGGAGTCCCATTTCAAGCAGACGAAGAAAGTGGTGGGTGGTATGGGAGTAGGGAAGTACAATCTCCTCCGGAAGAGGCAAATAAAGCTGGAGACCGAGGCGTGGGAGGAGGCGGCCAATGAGTACCAAGAGCTTTTAGTGGACATGTGTGAGCAGAAGCTTGCACCCAATTTGCCATACATTAAGTCATTGTTTCTTGGCTGGTTTGAGCCTTTACGAAACGCCATTGCAGAGGACCAAGAGGCCTGCAGACAACCGAACAGTCGAGCGAGTCATGCTCCGTATTTTGATCACTTACCGGCTGATAAGATGGCGGTTATCACAATGCACAAGTTGATGGGGCTGTTGATGACAAATAATGGCGGAGTAGGGAGTGTCAGGGTAGTCCAAGCTGCTTGTGCGATAGGTGAAGCCATTGAGAATGAGGTTAGGATACACAGGTTTTTGGAGAAGACAAAGAAGAAGAAGAACCTTACTGATAAGAAGCCTGAAGATGAATCTGATCCTGTGGCCATTGAACAAGAGAAACTAACGATCAGCGAGCAAGAGAAATTGACCAAAGAAAAAGAGAGACTTAGGAAAAAGGTTAACAAACTGATAAAAAAGCAGAAAATGAAGCAAGTAAGGGAGATTGTGAAGGAACAAGAAGACTTGAAGCCATGGGGTCAGGAAGCCCATGTTAAGGTTGGCTGTCGATTGATACAGTTGTTGATGGACACAGCTTATATACAACCTCCGGTTGATCAATCAGGAGATGCTTCACTCGATATACGCCCTGCATTTGTACATGTTCTTAAGACTGTCACTAAAGACACACAGAAGACTAGTAGGAGATATGGTATGATAGAATGCGACCCATTAGTTCACAAAGGCATGGAGAAAACTGCAAGGCACATGGTCATTCCTTATATGCCTATGTTGGTACCCCCTATCAACTGGACAGGGTACGACAGAGGCGCATATTTATTCTTACCATCCTATGTTATGCGAACTCATGGAGCGAAACAACAACGTCAAGTTGTTAAAAGGACACCTAGGACGCAATTAGAACCTGTATTTGAGGCCCTGGATACTCTTGGAAGTACCAAATGGAGGGTGAACAAACGGGTACTTGGTGTAATAGATAGGATATGGGCTAACGGAGGCCATCTTGCTGACTTGGTTGACCGTGAAGATGTTCCATTGCCTGAAGAACCGGACACAGAGGATGAGACAGAAATCAAAAAATGGAAGTGGAAAGTTAAAGATGCAAAGAAGGAGAATAGTGAGAGACACTCACAGCGGTGTGATATAGAACTCAAACTTGCTGTATCTCGAAAAATGAAGGATGAGGAAGGCTTCTACTACCCACACAACCTCGATTTTCGTGGTCGTGCTTACCCCATGCACCCATATCTAAACCATCTTGGTTCCGATATGTGCCGAGGCATCCTGGAGTTTTCAGAGGGACGGCGTCTTGGAAAGTCAGGCTTACGGTGGTTGAAGATACATTTGGCAAATTTATATGCTGGTGGTGTGGACAAACTCTCCTTTGATGGACGAGTAGCATTTACTGAGAATCATGTGGACGAAATTTTTGATTCGGCAGACAGATCTCTTGAAGGGAGGCGCTGGTGGTTGGGTGCGGAGGATCCTTTCCAATGCTTGGCAGCATGCATTAATCTCGCTGAAGCATTGAGAAGCCCCTCTCCAGAGACTACTGTTTCATATATGCCTGTCCACCAGGATGGTTCATGTAATGGTTTACAACATTATGCTGCTCTTGGAAGGGACAAGCTCGGAGCTGCTGCTGTTAATCTTGTGGGGGGAGAAAAACCTGCAGATGTTTACTCAGGAATTGCTGCCAGGGTACTCGATATAATGCGGAGCGATGCAGAAAAAGATCCTGCCACTAATCCAAATGCATTGCATGCTAGGCTTTTAGTCAATCAGGTGGATCGTAAATTGGTGAAGCAGACAGTTATGACGTCTGTATATGGCGTTACTTATGTTGGTGCACGTGATCAAATTAAGAGGAGATTAAAAGAGCGTGGTAACATAGCTGATGACTCGGCGCTGTTTGCTGCAGCTTGCTATGCTGCAAGGACCACCTTGACTGCACTAGGAGAGATGTTTGAAGCTGCAAGAAGTATTATGAGCTGGCTCGGTGAATGTGCAAAGGTGATAGCTTCGGAGAATCATCCAGTTCGGTGGACCACTCCCCTTGGACTTCCCGTTGTACAACCCTACCGACAATTAGGAAGGCATCTTATTAAGACTTCCCTTCAGGTGTTGACGTTACAAAGAGAAACTGACAAGGTCATGGTTAAACGGCAGAGGACAGCCTTTCCCCCAAATTTTGTTCACTCCCTTGACGGTTCCCACATGATGATGACTGCTGTTGCCTGCAAGAAAGCAGGCTTAAATTTTGCAGGAGTGCATGATTCATATTGGACGCATGCATGTGATGTTGACGAAATGAATAGGATACTAAGGGAGAAGTTTGTTGAACTCTACGAGGCACCTATACTGGAGAATTTGTTGGAGAGTTTTCAAGAGTCTTTCCCCAACTTGACTTTTCCGCCATTACCGGATCGTGGAGACTTCGATCTCAGAGATGTTCTCGAATCTCCCTATTTCTTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

1008

Amino Acids

114.18

Weight (kDa)

8.41

Isoelectric Point (pI)

49.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	158 - 493	6.8e-86	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	617 - 981	3.1e-157	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68990 AT1G68990
fragaria_vesca	FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970
malus_domestica	MD13G1055100.v1.1 MD16G1054000.v1.1
prunus_persica	Prupe.1G295700_v2.0.a1 Prupe.1G295700_v2.0.a1
pyrus_communis	pycom13g04880 pycom16g04700
rosa_chinensis	RchiOBHm_Chr4g0437111
rosa_laevigata	RLG00000006467
rosa_multiflora	Rmu_co8109606.1_g000001 Rmu_ssc0000006.1_g000008
rosa_roxburghii	Rroxscaffold_5G00378180
rosa_rugosa	Rorug04G0298100
rosa_samantha	Rh4AG352900 Rh4BG362400 Rh4CG376600 Rh4DG356400
rosa_wichuraiana	Rw4G030900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2366
Acc16I	TGCGCA	1 cut(s)	151
Acc36I	ACCTGC	1 cut(s)	2221
Acc65I	GGTACC	1 cut(s)	1318
AccB1I	GGYRCC	2 cut(s)	1318, 2896
AccB7I	CCANNNNNTGG	2 cut(s)	188, 2041
AccIII	TCCGGA	1 cut(s)	465
AciI	CCGC	8 cut(s)	300, 509, 710, 759, 1678, 2021, 2260, 2957
AclWI	GGATC	6 cut(s)	893, 2021, 2034, 2273, 2340, 2976
AcoI	YGGCCR	2 cut(s)	510, 906
AcsI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
AcuI	CTGAAG	5 cut(s)	369, 906, 1605, 2085, 2645
AcvI	CACGTG	1 cut(s)	2396
AcyI	GRCGYC	2 cut(s)	1849, 2363
AfaI	GTAC	9 cut(s)	455, 521, 1182, 1320, 1343, 1480, 1506, 2247, 2616
AfiI	CCNNNNNNNGG	7 cut(s)	188, 264, 498, 1078, 1488, 2041, 2972
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	2 cut(s)	540, 1183
AgsI	TTSAA	9 cut(s)	412, 914, 1051, 1879, 1997, 2513, 2885, 2930, 3019
AhlI	ACTAGT	1 cut(s)	1220
AjnI	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
AjuI	GAANNNNNNNTTGG	4 cut(s)	200, 232, 1871, 1903
AleI	CACNNNNGTG	1 cut(s)	1678
Alw21I	GWGCWC	1 cut(s)	2395
Alw26I	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
Alw44I	GTGCAC	1 cut(s)	2391
AlwI	GGATC	6 cut(s)	893, 2021, 2034, 2273, 2340, 2976
AlwNI	CAGNNNCTG	1 cut(s)	347
Ama87I	CYCGRG	1 cut(s)	116
Aor13HI	TCCGGA	1 cut(s)	465
AoxI	GGCC	7 cut(s)	138, 306, 510, 639, 906, 1458, 1540
ApaLI	GTGCAC	1 cut(s)	2391
ApoI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
ArsI	GACNNNNNNTTYG	2 cut(s)	1955, 1987
AseI	ATTAAT	1 cut(s)	2055
Asp700I	GAANNNNTTC	2 cut(s)	354, 1572
Asp718I	GGTACC	1 cut(s)	1318
AspA2I	CCTAGG	1 cut(s)	1430
AspLEI	GCGC	4 cut(s)	152, 1355, 2007, 2452
AspS9I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
AsuC2I	CCSGG	1 cut(s)	142
AsuHPI	GGTGA	7 cut(s)	100, 181, 815, 1504, 2356, 2555, 2569
AvaI	CYCGRG	1 cut(s)	116
AvaII	GGWCC	3 cut(s)	631, 2481, 2589
AvrII	CCTAGG	1 cut(s)	1430
BaeGI	GKGCMC	1 cut(s)	2395
BaeI	ACNNNNGTAYC	4 cut(s)	821, 854, 1088, 1121
BalI	TGGCCA	1 cut(s)	908
BamHI	GGATCC	1 cut(s)	2026
BanI	GGYRCC	2 cut(s)	1318, 2896
BbrPI	CACGTG	1 cut(s)	2396
BbsI	GAAGAC	4 cut(s)	339, 854, 1050, 1223
Bbv12I	GWGCWC	1 cut(s)	2395
BceAI	ACGGC	3 cut(s)	242, 1862, 2723
BcgI	CGANNNNNNTGC	4 cut(s)	293, 327, 2041, 2075
BciT130I	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BciVI	GTATCC	3 cut(s)	822, 1459, 2856
BclI	TGATCA	4 cut(s)	378, 685, 1138, 2398
BcnI	CCSGG	1 cut(s)	142
BcoDI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BcuI	ACTAGT	1 cut(s)	1220
BfaI	CTAG	5 cut(s)	1221, 1431, 2310, 2498, 3022
BfmI	CTRYAG	3 cut(s)	643, 2214, 2460
BfoI	RGCGCY	2 cut(s)	2008, 2453
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	1 cut(s)	2221
BfuI	GTATCC	3 cut(s)	822, 1459, 2856
BglI	GCCNNNNNGGC	1 cut(s)	305
BglII	AGATCT	1 cut(s)	1988
BlnI	CCTAGG	1 cut(s)	1430
Bme1390I	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
Bme18I	GGWCC	3 cut(s)	631, 2481, 2589
BmeT110I	CYCGRG	1 cut(s)	116
BmgT120I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
BmiI	GGNNCC	5 cut(s)	1320, 1807, 2028, 2755, 2898
BmrFI	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
BmsI	GCATC	6 cut(s)	215, 1141, 1633, 1833, 2257, 2650
BpiI	GAAGAC	4 cut(s)	339, 854, 1050, 1223
BpmI	CTGGAG	4 cut(s)	506, 1850, 2070, 2927
BpuMI	CCSGG	1 cut(s)	142
BsaAI	YACGTR	1 cut(s)	2396
BsaHI	GRCGYC	2 cut(s)	1849, 2363
BsaI	GGTCTC	1 cut(s)	482
BsaJI	CCNNGG	8 cut(s)	303, 492, 1055, 1430, 1461, 1819, 2241, 2599
BsaWI	WCCGGW	4 cut(s)	465, 1132, 1591, 2965
BsaXI	ACNNNNNCTCC	6 cut(s)	1219, 1249, 1907, 1937, 2900, 2930
Bsc4I	CCNNNNNNNGG	7 cut(s)	188, 264, 498, 1078, 1488, 2041, 2972
Bse118I	RCCGGY	1 cut(s)	694
Bse1I	ACTGG	4 cut(s)	294, 1337, 2579, 2910
Bse3DI	GCAATG	4 cut(s)	309, 621, 1577, 2300
BseAI	TCCGGA	1 cut(s)	465
BseBI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BseDI	CCNNGG	8 cut(s)	303, 492, 1055, 1430, 1461, 1819, 2241, 2599
BseGI	GGATG	7 cut(s)	328, 1370, 1609, 1729, 1824, 2128, 2575
BseLI	CCNNNNNNNGG	7 cut(s)	188, 264, 498, 1078, 1488, 2041, 2972
BseMI	GCAATG	4 cut(s)	309, 621, 1577, 2300
BseMII	CTCAG	3 cut(s)	1941, 2241, 3001
BseNI	ACTGG	4 cut(s)	294, 1337, 2579, 2910
BseRI	GAGGAG	3 cut(s)	452, 518, 2425
BseSI	GKGCMC	1 cut(s)	2395
BsgI	GTGCAG	1 cut(s)	2478
BshFI	GGCC	7 cut(s)	140, 308, 512, 641, 908, 1460, 1542
BshNI	GGYRCC	2 cut(s)	1318, 2896
BsiHKAI	GWGCWC	1 cut(s)	2395
BsiHKCI	CYCGRG	1 cut(s)	116
BsiSI	CCGG	6 cut(s)	142, 466, 695, 1133, 1592, 2966
BslFI	GGGAC	3 cut(s)	388, 1857, 2180
BslI	CCNNNNNNNGG	7 cut(s)	188, 264, 498, 1078, 1488, 2041, 2972
BsmAI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BsmFI	GGGAC	3 cut(s)	388, 1857, 2180
BsmI	GAATGC	1 cut(s)	1250
BsnI	GGCC	7 cut(s)	140, 308, 512, 641, 908, 1460, 1542
Bso31I	GGTCTC	1 cut(s)	482
BsoBI	CYCGRG	1 cut(s)	116
Bsp1286I	GDGCHC	1 cut(s)	2395
Bsp13I	TCCGGA	1 cut(s)	465
Bsp1407I	TGTACA	2 cut(s)	1180, 2614
Bsp19I	CCATGG	1 cut(s)	1055
BspACI	CCGC	8 cut(s)	300, 509, 710, 759, 1678, 2021, 2260, 2957
BspANI	GGCC	7 cut(s)	140, 308, 512, 641, 908, 1460, 1542
BspCNI	CTCAG	3 cut(s)	1942, 2240, 3000
BspEI	TCCGGA	1 cut(s)	465
BspLI	GGNNCC	5 cut(s)	1320, 1807, 2028, 2755, 2898
BspMAI	CTGCAG	3 cut(s)	647, 2218, 2464
BspMI	ACCTGC	1 cut(s)	2221
BspPI	GGATC	6 cut(s)	893, 2021, 2034, 2273, 2340, 2976
BspT107I	GGYRCC	2 cut(s)	1318, 2896
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	482
BsrDI	GCAATG	4 cut(s)	309, 621, 1577, 2300
BsrFI	RCCGGY	1 cut(s)	694
BsrGI	TGTACA	2 cut(s)	1180, 2614
BsrI	ACTGG	4 cut(s)	294, 1337, 2579, 2910
BssAI	RCCGGY	1 cut(s)	694
BssECI	CCNNGG	8 cut(s)	303, 492, 1055, 1430, 1461, 1819, 2241, 2599
BssNI	GRCGYC	2 cut(s)	1849, 2363
BssT1I	CCWWGG	3 cut(s)	1055, 1430, 2599
Bst2UI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
Bst4CI	ACNGT	9 cut(s)	57, 659, 1101, 1198, 1565, 1872, 2098, 2356, 2753
Bst6I	CTCTTC	1 cut(s)	464
BstACI	GRCGYC	2 cut(s)	1849, 2363
BstAFI	CTTAAG	1 cut(s)	1190
BstAPI	GCANNNNNTGC	1 cut(s)	2306
BstAUI	TGTACA	2 cut(s)	1180, 2614
BstBAI	YACGTR	1 cut(s)	2396
BstDEI	CTNAG	5 cut(s)	971, 1950, 2227, 2867, 2987
BstDSI	CCRYGG	1 cut(s)	1055
BstF5I	GGATG	7 cut(s)	328, 1370, 1609, 1729, 1824, 2128, 2575
BstH2I	RGCGCY	2 cut(s)	2008, 2453
BstHHI	GCGC	4 cut(s)	152, 1355, 2007, 2452
BstMAI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BstMWI	GCNNNNNNNGC	9 cut(s)	299, 305, 481, 671, 794, 2306, 2311, 2456, 2471
BstNI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BstNSI	RCATGY	6 cut(s)	544, 1187, 2052, 2309, 2837, 2841
BstSCI	CCNGG	6 cut(s)	125, 140, 1461, 1827, 2118, 2240
BstSFI	CTRYAG	3 cut(s)	643, 2214, 2460
BstSLI	GKGCMC	1 cut(s)	2395
BstV2I	GAAGAC	4 cut(s)	339, 854, 1050, 1223
BstX2I	RGATCY	3 cut(s)	1988, 2026, 2278
BstXI	CCANNNNNNTGG	2 cut(s)	2123, 2586
BstYI	RGATCY	3 cut(s)	1988, 2026, 2278
BsuI	GTATCC	3 cut(s)	822, 1459, 2856
BsuRI	GGCC	7 cut(s)	140, 308, 512, 641, 908, 1460, 1542
BtgI	CCRYGG	1 cut(s)	1055
BtgZI	GCGATG	1 cut(s)	2280
BtsCI	GGATG	7 cut(s)	328, 1370, 1609, 1729, 1824, 2128, 2575
BtsIMutI	CAGTG	1 cut(s)	301
BveI	ACCTGC	1 cut(s)	2221
CaiI	CAGNNNCTG	1 cut(s)	347
CfoI	GCGC	4 cut(s)	152, 1355, 2007, 2452
Cfr10I	RCCGGY	1 cut(s)	694
Cfr13I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
CseI	GACGC	3 cut(s)	1444, 1838, 2839
Csp6I	GTAC	9 cut(s)	454, 520, 1181, 1319, 1342, 1479, 1505, 2246, 2615
CspCI	CAANNNNNGTGG	2 cut(s)	2581, 2616
CviQI	GTAC	9 cut(s)	454, 520, 1181, 1319, 1342, 1479, 1505, 2246, 2615
DdeI	CTNAG	5 cut(s)	971, 1950, 2227, 2867, 2987
EaeI	YGGCCR	2 cut(s)	510, 906
Eam1104I	CTCTTC	1 cut(s)	464
EarI	CTCTTC	1 cut(s)	464
EciI	GGCGGA	2 cut(s)	774, 2946
Eco130I	CCWWGG	3 cut(s)	1055, 1430, 2599
Eco147I	AGGCCT	1 cut(s)	641
Eco31I	GGTCTC	1 cut(s)	482
Eco47I	GGWCC	3 cut(s)	631, 2481, 2589
Eco57I	CTGAAG	5 cut(s)	369, 906, 1605, 2085, 2645
Eco72I	CACGTG	1 cut(s)	2396
Eco88I	CYCGRG	1 cut(s)	116
EcoO109I	RGGNCCY	1 cut(s)	1459
EcoRII	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
EcoT14I	CCWWGG	3 cut(s)	1055, 1430, 2599
EcoT22I	ATGCAT	3 cut(s)	2054, 2302, 2839
ErhI	CCWWGG	3 cut(s)	1055, 1430, 2599
FalI	AAGNNNNNCTT	4 cut(s)	954, 986, 1850, 1882
FaqI	GGGAC	3 cut(s)	388, 1857, 2180
FbaI	TGATCA	4 cut(s)	378, 685, 1138, 2398
FokI	GGATG	7 cut(s)	335, 1357, 1616, 1736, 1811, 2135, 2562
FspBI	CTAG	5 cut(s)	1221, 1431, 2310, 2498, 3022
FspI	TGCGCA	1 cut(s)	151
GlaI	GCGC	4 cut(s)	151, 1354, 2006, 2451
GsuI	CTGGAG	4 cut(s)	506, 1850, 2070, 2927
HaeII	RGCGCY	2 cut(s)	2008, 2453
HaeIII	GGCC	7 cut(s)	140, 308, 512, 641, 908, 1460, 1542
HapII	CCGG	6 cut(s)	142, 466, 695, 1133, 1592, 2966
HgaI	GACGC	3 cut(s)	1444, 1838, 2839
HhaI	GCGC	4 cut(s)	152, 1355, 2007, 2452
Hin1I	GRCGYC	2 cut(s)	1849, 2363
Hin6I	GCGC	4 cut(s)	150, 1353, 2005, 2450
HinP1I	GCGC	4 cut(s)	150, 1353, 2005, 2450
HincII	GTYRAC	4 cut(s)	985, 1561, 2670, 2848
HindII	GTYRAC	4 cut(s)	985, 1561, 2670, 2848
HindIII	AAGCTT	2 cut(s)	198, 554
HpaI	GTTAAC	1 cut(s)	985
HpaII	CCGG	6 cut(s)	142, 466, 695, 1133, 1592, 2966
HphI	GGTGA	7 cut(s)	100, 181, 815, 1504, 2356, 2555, 2569
Hpy188I	TCNGA	9 cut(s)	243, 349, 383, 898, 1810, 1840, 2177, 2569, 2990
Hpy99I	CGWCG	1 cut(s)	322
HpyAV	CCTTC	9 cut(s)	202, 1027, 1645, 1714, 1724, 1991, 2157, 2631, 2669
HpyCH4III	ACNGT	9 cut(s)	57, 659, 1101, 1198, 1565, 1872, 2098, 2356, 2753
HpyCH4IV	ACGT	4 cut(s)	1408, 2363, 2395, 2672
HpyF10VI	GCNNNNNNNGC	9 cut(s)	299, 305, 481, 671, 794, 2306, 2311, 2456, 2471
HpyF3I	CTNAG	5 cut(s)	971, 1950, 2227, 2867, 2987
HpySE526I	ACGT	4 cut(s)	1408, 2363, 2395, 2672
Hsp92I	GRCGYC	2 cut(s)	1849, 2363
HspAI	GCGC	4 cut(s)	150, 1353, 2005, 2450
Kpn2I	TCCGGA	1 cut(s)	465
KpnI	GGTACC	1 cut(s)	1322
Ksp22I	TGATCA	4 cut(s)	378, 685, 1138, 2398
KspAI	GTTAAC	1 cut(s)	985
LmnI	GCTCC	4 cut(s)	679, 1394, 2177, 2262
LweI	GCATC	6 cut(s)	215, 1141, 1633, 1833, 2257, 2650
MaeI	CTAG	5 cut(s)	1221, 1431, 2310, 2498, 3022
MaeII	ACGT	4 cut(s)	1408, 2363, 2395, 2672
MaeIII	GTNAC	5 cut(s)	245, 1198, 2377, 2429, 2673
MflI	RGATCY	3 cut(s)	1988, 2026, 2278
MhlI	GDGCHC	1 cut(s)	2395
MlsI	TGGCCA	1 cut(s)	908
MluNI	TGGCCA	1 cut(s)	908
MlyI	GAGTC	5 cut(s)	329, 410, 676, 2438, 2942
MmeI	TCCRAC	2 cut(s)	348, 2898
Mox20I	TGGCCA	1 cut(s)	908
Mph1103I	ATGCAT	3 cut(s)	2054, 2302, 2839
MroI	TCCGGA	1 cut(s)	465
MroXI	GAANNNNTTC	2 cut(s)	354, 1572
MscI	TGGCCA	1 cut(s)	908
MslI	CAYNNNNRTG	4 cut(s)	1678, 2121, 2765, 2838
Msp20I	TGGCCA	1 cut(s)	908
MspA1I	CMGCKG	1 cut(s)	1678
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	6 cut(s)	142, 466, 695, 1133, 1592, 2966
MspR9I	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
Mva1269I	GAATGC	1 cut(s)	1250
MvaI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
MwoI	GCNNNNNNNGC	9 cut(s)	299, 305, 481, 671, 794, 2306, 2311, 2456, 2471
NciI	CCSGG	1 cut(s)	142
NcoI	CCATGG	1 cut(s)	1055
NlaIV	GGNNCC	5 cut(s)	1320, 1807, 2028, 2755, 2898
NmeAIII	GCCGAG	3 cut(s)	328, 1844, 2426
NmuCI	GTSAC	1 cut(s)	1198
NsbI	TGCGCA	1 cut(s)	151
NsiI	ATGCAT	3 cut(s)	2054, 2302, 2839
NspI	RCATGY	6 cut(s)	544, 1187, 2052, 2309, 2837, 2841
OliI	CACNNNNGTG	1 cut(s)	1678
PaeI	GCATGC	3 cut(s)	2052, 2309, 2837
PaeR7I	CTCGAG	1 cut(s)	116
PceI	AGGCCT	1 cut(s)	641
PciI	ACATGT	2 cut(s)	540, 1183
PctI	GAATGC	1 cut(s)	1250
PdmI	GAANNNNTTC	2 cut(s)	354, 1572
PfeI	GAWTC	8 cut(s)	104, 238, 893, 1954, 1976, 2572, 2819, 3002
PflFI	GACNNNGTC	2 cut(s)	1848, 2693
PflMI	CCANNNNNTGG	2 cut(s)	188, 2041
PfoI	TCCNGGA	1 cut(s)	1827
PleI	GAGTC	5 cut(s)	329, 409, 675, 2438, 2941
PmaCI	CACGTG	1 cut(s)	2396
PmlI	CACGTG	1 cut(s)	2396
PpsI	GAGTC	5 cut(s)	329, 409, 675, 2438, 2941
Ppu21I	YACGTR	1 cut(s)	2396
PscI	ACATGT	2 cut(s)	540, 1183
PshBI	ATTAAT	1 cut(s)	2055
Psp6I	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
PspCI	CACGTG	1 cut(s)	2396
PspGI	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
PspN4I	GGNNCC	5 cut(s)	1320, 1807, 2028, 2755, 2898
PspPI	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
PstI	CTGCAG	3 cut(s)	647, 2218, 2464
PstNI	CAGNNNCTG	1 cut(s)	347
PsuI	RGATCY	3 cut(s)	1988, 2026, 2278
PsyI	GACNNNGTC	2 cut(s)	1848, 2693
RsaI	GTAC	9 cut(s)	455, 521, 1182, 1320, 1343, 1480, 1506, 2247, 2616
RsaNI	GTAC	9 cut(s)	454, 520, 1181, 1319, 1342, 1479, 1505, 2246, 2615
RseI	CAYNNNNRTG	4 cut(s)	1678, 2121, 2765, 2838
Sau96I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
SchI	GAGTC	5 cut(s)	329, 410, 676, 2438, 2942
ScrFI	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
SduI	GDGCHC	1 cut(s)	2395
SfaNI	GCATC	6 cut(s)	215, 1141, 1633, 1833, 2257, 2650
SfcI	CTRYAG	3 cut(s)	643, 2214, 2460
Sfr274I	CTCGAG	1 cut(s)	116
SinI	GGWCC	3 cut(s)	631, 2481, 2589
SlaI	CTCGAG	1 cut(s)	116
SmiMI	CAYNNNNRTG	4 cut(s)	1678, 2121, 2765, 2838
SmlI	CTYRAG	2 cut(s)	116, 1190
SmoI	CTYRAG	2 cut(s)	116, 1190
SpeI	ACTAGT	1 cut(s)	1220
SphI	GCATGC	3 cut(s)	2052, 2309, 2837
SseBI	AGGCCT	1 cut(s)	641
SsiI	CCGC	8 cut(s)	300, 509, 710, 759, 1678, 2021, 2260, 2957
SspMI	CTAG	5 cut(s)	1221, 1431, 2310, 2498, 3022
StuI	AGGCCT	1 cut(s)	641
StyD4I	CCNGG	6 cut(s)	125, 140, 1461, 1827, 2118, 2240
StyI	CCWWGG	3 cut(s)	1055, 1430, 2599
TaaI	ACNGT	9 cut(s)	57, 659, 1101, 1198, 1565, 1872, 2098, 2356, 2753
TaiI	ACGT	4 cut(s)	1411, 2366, 2398, 2675
TaqII	GACCGA	1 cut(s)	506
TatI	WGTACW	3 cut(s)	453, 1180, 2614
TauI	GCSGC	2 cut(s)	302, 512
TfiI	GAWTC	8 cut(s)	104, 238, 893, 1954, 1976, 2572, 2819, 3002
TscAI	CASTG	1 cut(s)	301
TseFI	GTSAC	1 cut(s)	1198
Tsp45I	GTSAC	1 cut(s)	1198
TspDTI	ATGAA	9 cut(s)	50, 251, 906, 1025, 1733, 2091, 2118, 2811, 2870
TspGWI	ACGGA	3 cut(s)	335, 666, 1551
TspRI	CASTG	1 cut(s)	301
Tth111I	GACNNNGTC	2 cut(s)	1848, 2693
Van91I	CCANNNNNTGG	2 cut(s)	188, 2041
Vha464I	CTTAAG	1 cut(s)	1190
VneI	GTGCAC	1 cut(s)	2391
VpaK11BI	GGWCC	3 cut(s)	631, 2481, 2589
VspI	ATTAAT	1 cut(s)	2055
XapI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
XceI	RCATGY	6 cut(s)	544, 1187, 2052, 2309, 2837, 2841
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1079
XhoI	CTCGAG	1 cut(s)	116
XmaJI	CCTAGG	1 cut(s)	1430
XmnI	GAANNNNTTC	2 cut(s)	354, 1572
XspI	CTAG	5 cut(s)	1221, 1431, 2310, 2498, 3022
ZraI	GACGTC	1 cut(s)	2364
Zsp2I	ATGCAT	3 cut(s)	2054, 2302, 2839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.