Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0007623

Physical Location & Seq

Genomic Coordinates Reverse (-)

52102261 .. 52110327

Gene Structure 8067 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG362400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3027 bp

ATGTGGAGAACCCTAGCCAAACAAGCTGCTTCTTCTTCTTCTTCAATAAAGACCAAGCTTTTCTCTCCCTCTCCATTCAGCTCTGCTCTCAAGTTTTCCAAAGAATCGAGCTTTCTGGAGAAAACCAGGCATTTTGAGGCTCGCGTCTGCAGAAATTATGGGTCTTCGTTAATGGGTTTAGGCCAGGTTGGTGGTGCATCGTCCCAGAAAGACGAATTGGGCAGACCCAGTCTGCTGAATCCGAGTTACCCTTGTGGGTTTGGGAGATGGTATGCTACTTCTGCCGAGGCTATTGCTTCCACGGATGAGGAATCTGGGTCTGAAGAGATTCAGGAAATGCTGGAGGATTTGGTGAGGGAGAACAAGGTGGAGTCGCATTTGAGGCAGCCCAAGAGGTTGGTTAATGGAATGGGAGCAGGAAAGTATGGCATTCTGAGAAAGAGGCAGTTAAAAATTGAGACTGAGGCTTGGGAAGATGCGGCGAAAGAGTACCAGGAGCTTTTGGCTGATATGTGTGAGCAGAAGCTGGCGCCGAATTTGCCTTATGTGAAGTCATTGTTTCTGGGGTGGTTTGAGCCATTGAGGGATGCCATTGCCGCCGAGCCAGCGTCTTGGGCCAGTAAGAGGGTGCATATCAGACCCAGCCATGCTCCGTTTTTCGAACTGTTGCCGCCGGATAAGATGGCTGTTATTACAATGCATAAGTTGATGGGGCTGTTGATGACCAACAGTGGTGGAGTTGGGTCTGTCAAGGTGGTTCAAGCTGCTTGTGCAATTGGTGAAGCTCTTGAGAATGAGGTTAAGATACACAGATTTTTGGAGAAGACAAAGAAGAAGAGCATTGTGAGTAAGAAACCTGAAGCTGAATCTGATATTGTGACCAATGAACAAGAGAAACTTACCAACGAACAGGAGCAAGAGAAACTAACCAAAGAACAGGAGCTAGAAAAACTAACCAAAGAACAAAGACTGAGGAAAAAGGTTACAAAGTTGCTAAAAATGCAGAAGCTTCAGCGAGTAAAGGAAATTGTGAAGGAACAAGACGGCTTAAAGCCATGGGGTCAAGAAGCTCATGTTAAGGTCGGCTGTCGATTGATTCAGTTAATGATGGAAACAGCTTATATACAACCTGCACTTGATCAATTAGGAGATGCTCCGCCTGATATTCGCCCGGCATTCGTGCATACCCTTAAGACTATAACTAAAGAGGCACAGAAGACCAACAGGAGATATGGTGTTATTGAATGCGACCCATTAGTTCGCAAAGGGATGGAGAAAACAGCAAGGCACATGGTCATTCCTTATATGCCAATGTTGGTGCCTCCACTTAACTGGACAGGGTATGATAAAGGAGCATATTTATTCTTACCATCCTATGTTATGCGAACTCATGGAGCGAAACAACAGCGCCAAGTTGTTAAATCGACTCCTAGGAAGCAATTAGAACCTGTTTATGAGGCCCTGGATACTCTTGGAAATACCAAATGGAGGATAAACAGAAGGGTACTTGGTGTAGTGGACAGGATATGGGCTAGTGGAGGCTGTCTTGCTGACTTGGTTGACCGTGAAGATGTTCCTTTGCCTGAAGAACCAGACACAGAAGATGAGGCAGAAATGAGAAAATGGAAATGGAAAGTTAAAGCTGTGACAAAGAAGAATAGTGAGAGGCACTCACAGCGTTGTGATACAGAACTTAAACTTTCTCAGGTAGCTCGAAAGATGAAGGACGAGGAAGGCTTCTACTATCCGCACAATCTTGATTTCCGTGGTCGTGCCTACCCCATGCACCCATATCTAAACCATCTTGGTTCCGATATGTGCAGAGGCGTCCTGGAGTTCGCAGAGGGACGTCGTCTTGGAAAATCTGGCTTACGCTGGTTGAAGATACATCTGGCAAATTTATATGCTGGTGGAGTTGACAAGCTCTCCTATGATGATCGAGTAACATTTACGGAAAACCATGTAGACGAAATTTTTGATTGCGCAGACAGACCTCTTGAAGGAAGGCGTTGGTGGTTGGGTGCGGAAGATCCTTTCCAATGCTTGGCAGCATGCATTAATCTCACTGAAGCATTGAGAAGCCCCTCACCGGAGACTACCATTTCTCATATGCCTGTTCACCAGGATGGTTCATGTAATGGTTTACAACACTATGCTGCCCTCGGAAGGGACAAGCTTGGAGCAGCTGCTGTAAATCTTGTTGGGGGAGAGAAACCGGCTGATGTTTACTCAGGAATTGCTGCCAGGGTACTCGATATAATGCGCACAGATGCAGAGAAAGATCCAGCCAGTAATCCAACTTCATTGCACGCTAGGCTTTTAATTAATGAGGTCGATCGTAAATTGGTGAAGCAGACAGTTATGACCTCTGTATATGGTGTTACTTATATTGGTGCACGCGATCAAATTAAGAAGAGATTAAAGGAGCGTGGTACCATAGCAGATGACGCGATGCTGTTTGCCGCATCTTGCTATGCTGCTAGAACCACCTTGACTGCCCTAGGAGAGATGTTTGAAGCTGCAAGAAGTATTATGAGTTGGCTTGGTGAATGTGCGAAGGTGATAGCTGCAGAGAATCATCCAGTTCGATGGACCACACCCCTTGGACTTCCTGTTGTACAACCATACCGCCAATTAGGAAGGCATCTTATTAAGACTTCCCTTCAGGTGCTGACATTACAGAGAGAAACCGACAAGGTCATGGTTAAACGGCAGAGGACAGCCTTTCCCCCCAATTTTGTCCACTCCCTTGATGGATCCCACATGATGATGACTGCTCTTGCCTGCAAGAAGGCAGGCTTAAATTTTGCAGGAGTACATGATTCATATTGGACGCATGCAAGTGACGTTGACGAAATGAATAGGATACTGAGAGAAAAGTTTGTTGAACTCTACGAGAGACCAATACTGGAGAATTTGTTGGAGAGCTTTCAAAAGTCTTTCCCCACCATTAATTTTCCTCCCTTACCGGATCGCGGAGACTTTGATCTCAGACAGGTGCTCGGATCTACTTATTTCTTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

1008

Amino Acids

114.04

Weight (kDa)

8.94

Isoelectric Point (pI)

44.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	148 - 493	6.2e-84	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	618 - 982	3.7e-158	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68990 AT1G68990
fragaria_vesca	FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970
malus_domestica	MD13G1055100.v1.1 MD16G1054000.v1.1
prunus_persica	Prupe.1G295700_v2.0.a1 Prupe.1G295700_v2.0.a1
pyrus_communis	pycom13g04880 pycom16g04700
rosa_chinensis	RchiOBHm_Chr4g0437111
rosa_laevigata	RLG00000006467
rosa_multiflora	Rmu_co8109606.1_g000001 Rmu_ssc0000006.1_g000008
rosa_roxburghii	Rroxscaffold_5G00378180
rosa_rugosa	Rorug04G0298100
rosa_samantha	Rh4AG352900 Rh4BG362400 Rh4CG376600 Rh4DG356400
rosa_wichuraiana	Rw4G030900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1852
Acc16I	TGCGCA	2 cut(s)	1984, 2264
Acc36I	ACCTGC	1 cut(s)	1138
Acc65I	GGTACC	1 cut(s)	2432
AccB1I	GGYRCC	3 cut(s)	529, 1318, 2432
AccB7I	CCANNNNNTGG	1 cut(s)	2044
AccI	GTMKAC	1 cut(s)	1965
AccII	CGCG	4 cut(s)	144, 2400, 2450, 2976
AciI	CCGC	9 cut(s)	479, 597, 671, 1157, 1748, 2024, 2463, 2629, 2976
AclWI	GGATC	6 cut(s)	2024, 2276, 2751, 2764, 2979, 3013
AcsI	RAATTY	5 cut(s)	535, 1897, 1971, 2803, 2914
AcuI	CTGAAG	6 cut(s)	342, 879, 995, 1605, 2088, 2648
AcyI	GRCGYC	3 cut(s)	530, 1827, 1849
AfaI	GTAC	6 cut(s)	491, 1506, 2250, 2434, 2619, 2817
AfiI	CCNNNNNNNGG	8 cut(s)	624, 1488, 2000, 2044, 2089, 2166, 2167, 2975
AflII	CTTAAG	1 cut(s)	1190
AgsI	TTSAA	9 cut(s)	45, 761, 1244, 1882, 2000, 2516, 2888, 2933, 3022
AjnI	CCWGG	7 cut(s)	125, 183, 492, 1461, 1830, 2121, 2243
AjuI	GAANNNNNNNTTGG	2 cut(s)	200, 232
Alw21I	GWGCWC	2 cut(s)	2398, 3003
Alw26I	GTCTC	4 cut(s)	452, 2087, 2893, 2973
Alw44I	GTGCAC	1 cut(s)	2394
AlwI	GGATC	6 cut(s)	2024, 2276, 2751, 2764, 2979, 3013
AlwNI	CAGNNNCTG	3 cut(s)	526, 2189, 2671
AoxI	GGCC	3 cut(s)	181, 615, 1458
ApaLI	GTGCAC	1 cut(s)	2394
ApoI	RAATTY	5 cut(s)	535, 1897, 1971, 2803, 2914
ArsI	GACNNNNNNTTYG	2 cut(s)	1958, 1990
AseI	ATTAAT	3 cut(s)	2058, 2325, 2952
Asp700I	GAANNNNTTC	2 cut(s)	327, 1572
Asp718I	GGTACC	1 cut(s)	2432
AspA2I	CCTAGG	2 cut(s)	1430, 2500
AspLEI	GCGC	4 cut(s)	532, 1410, 1985, 2265
AspS9I	GGNCC	3 cut(s)	615, 1459, 2592
AsuC2I	CCSGG	1 cut(s)	1172
AsuHPI	GGTGA	7 cut(s)	364, 791, 2079, 2111, 2359, 2558, 2572
AsuII	TTCGAA	1 cut(s)	660
AvaII	GGWCC	1 cut(s)	2592
AvrII	CCTAGG	2 cut(s)	1430, 2500
BaeGI	GKGCMC	1 cut(s)	2398
BamHI	GGATCC	1 cut(s)	2756
BanI	GGYRCC	3 cut(s)	529, 1318, 2432
BarI	GAAGNNNNNNTAC	2 cut(s)	2284, 2316
BbsI	GAAGAC	3 cut(s)	156, 830, 1223
Bbv12I	GWGCWC	2 cut(s)	2398, 3003
BceAI	ACGGC	2 cut(s)	1060, 2726
BcgI	CGANNNNNNTGC	2 cut(s)	275, 309
BciT130I	CCWGG	7 cut(s)	127, 185, 494, 1463, 1832, 2123, 2245
BciVI	GTATCC	2 cut(s)	1459, 2859
BclI	TGATCA	1 cut(s)	1138
BcnI	CCSGG	1 cut(s)	1172
BcoDI	GTCTC	4 cut(s)	452, 2087, 2893, 2973
BfaI	CTAG	8 cut(s)	14, 944, 1431, 1533, 2313, 2481, 2501, 3025
BfmI	CTRYAG	2 cut(s)	148, 2568
BfoI	RGCGCY	2 cut(s)	533, 1411
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	1 cut(s)	1138
BfuI	GTATCC	2 cut(s)	1459, 2859
BlnI	CCTAGG	2 cut(s)	1430, 2500
Bme1390I	CCNGG	8 cut(s)	127, 185, 494, 1172, 1463, 1832, 2123, 2245
Bme18I	GGWCC	1 cut(s)	2592
BmgT120I	GGNCC	3 cut(s)	615, 1459, 2592
BmiI	GGNNCC	5 cut(s)	531, 1320, 1810, 2434, 2758
BmrFI	CCNGG	8 cut(s)	127, 185, 494, 1172, 1463, 1832, 2123, 2245
BmrI	ACTGGG	1 cut(s)	222
BmsI	GCATC	8 cut(s)	206, 466, 577, 1141, 2260, 2442, 2474, 2653
BmuI	ACTGGG	1 cut(s)	222
BpiI	GAAGAC	3 cut(s)	156, 830, 1223
BplI	GAGNNNNNCTC	2 cut(s)	1655, 1687
BpmI	CTGGAG	4 cut(s)	137, 362, 1853, 2930
Bpu14I	TTCGAA	1 cut(s)	660
BpuEI	CTTGAG	2 cut(s)	74, 809
BpuMI	CCSGG	1 cut(s)	1172
BsaHI	GRCGYC	3 cut(s)	530, 1827, 1849
BsaI	GGTCTC	1 cut(s)	2893
BsaWI	WCCGGW	2 cut(s)	2089, 2968
BsaXI	ACNNNNNCTCC	6 cut(s)	1219, 1249, 1910, 1940, 2903, 2933
Bsc4I	CCNNNNNNNGG	8 cut(s)	624, 1488, 2000, 2044, 2089, 2166, 2167, 2975
Bse118I	RCCGGY	1 cut(s)	2215
Bse1I	ACTGG	6 cut(s)	228, 618, 1337, 2289, 2582, 2913
Bse3DI	GCAATG	2 cut(s)	591, 2303
BseBI	CCWGG	7 cut(s)	127, 185, 494, 1463, 1832, 2123, 2245
BseGI	GGATG	6 cut(s)	310, 592, 1275, 1370, 2131, 2578
BseLI	CCNNNNNNNGG	8 cut(s)	624, 1488, 2000, 2044, 2089, 2166, 2167, 2975
BseMI	GCAATG	2 cut(s)	591, 2303
BseMII	CTCAG	7 cut(s)	425, 453, 962, 1718, 2244, 2861, 3004
BseNI	ACTGG	6 cut(s)	228, 618, 1337, 2289, 2582, 2913
BseSI	GKGCMC	1 cut(s)	2398
BseYI	CCCAGC	1 cut(s)	641
BsgI	GTGCAG	2 cut(s)	1116, 1840
Bsh1236I	CGCG	4 cut(s)	144, 2400, 2450, 2976
Bsh1285I	CGRYCG	1 cut(s)	2338
BshFI	GGCC	3 cut(s)	183, 617, 1460
BshNI	GGYRCC	3 cut(s)	529, 1318, 2432
BsiEI	CGRYCG	1 cut(s)	2338
BsiHKAI	GWGCWC	2 cut(s)	2398, 3003
BsiSI	CCGG	5 cut(s)	674, 1172, 2090, 2216, 2969
BslFI	GGGAC	3 cut(s)	187, 1860, 2183
BslI	CCNNNNNNNGG	8 cut(s)	624, 1488, 2000, 2044, 2089, 2166, 2167, 2975
BsmAI	GTCTC	4 cut(s)	452, 2087, 2893, 2973
BsmFI	GGGAC	3 cut(s)	187, 1860, 2183
BsmI	GAATGC	3 cut(s)	429, 1175, 1250
BsnI	GGCC	3 cut(s)	183, 617, 1460
Bso31I	GGTCTC	1 cut(s)	2893
Bsp119I	TTCGAA	1 cut(s)	660
Bsp1286I	GDGCHC	2 cut(s)	2398, 3003
Bsp1407I	TGTACA	1 cut(s)	2617
Bsp19I	CCATGG	1 cut(s)	1055
BspACI	CCGC	9 cut(s)	479, 597, 671, 1157, 1748, 2024, 2463, 2629, 2976
BspANI	GGCC	3 cut(s)	183, 617, 1460
BspCNI	CTCAG	7 cut(s)	426, 454, 963, 1717, 2243, 2862, 3003
BspFNI	CGCG	4 cut(s)	144, 2400, 2450, 2976
BspLI	GGNNCC	5 cut(s)	531, 1320, 1810, 2434, 2758
BspMAI	CTGCAG	2 cut(s)	152, 2572
BspMI	ACCTGC	1 cut(s)	1138
BspPI	GGATC	6 cut(s)	2024, 2276, 2751, 2764, 2979, 3013
BspQI	GCTCTTC	1 cut(s)	830
BspT104I	TTCGAA	1 cut(s)	660
BspT107I	GGYRCC	3 cut(s)	529, 1318, 2432
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	2893
BsrDI	GCAATG	2 cut(s)	591, 2303
BsrFI	RCCGGY	1 cut(s)	2215
BsrGI	TGTACA	1 cut(s)	2617
BsrI	ACTGG	6 cut(s)	228, 618, 1337, 2289, 2582, 2913
BssAI	RCCGGY	1 cut(s)	2215
BssNI	GRCGYC	3 cut(s)	530, 1827, 1849
BssT1I	CCWWGG	4 cut(s)	1055, 1430, 2500, 2602
Bst2UI	CCWGG	7 cut(s)	127, 185, 494, 1463, 1832, 2123, 2245
Bst4CI	ACNGT	4 cut(s)	666, 731, 1565, 2359
Bst6I	CTCTTC	3 cut(s)	318, 830, 2408
BstACI	GRCGYC	3 cut(s)	530, 1827, 1849
BstAFI	CTTAAG	1 cut(s)	1190
BstAUI	TGTACA	1 cut(s)	2617
BstBI	TTCGAA	1 cut(s)	660
BstC8I	GCNNGC	9 cut(s)	142, 528, 606, 2053, 2310, 2398, 2785, 2797, 2838
BstDEI	CTNAG	7 cut(s)	434, 462, 971, 1704, 2230, 2870, 2990
BstDSI	CCRYGG	3 cut(s)	300, 1055, 1765
BstENI	CCTNNNNNAGG	1 cut(s)	1998
BstF5I	GGATG	6 cut(s)	310, 592, 1275, 1370, 2131, 2578
BstFNI	CGCG	4 cut(s)	144, 2400, 2450, 2976
BstH2I	RGCGCY	2 cut(s)	533, 1411
BstHHI	GCGC	4 cut(s)	532, 1410, 1985, 2265
BstMAI	GTCTC	4 cut(s)	452, 2087, 2893, 2973
BstMCI	CGRYCG	1 cut(s)	2338
BstNI	CCWGG	7 cut(s)	127, 185, 494, 1463, 1832, 2123, 2245
BstNSI	RCATGY	2 cut(s)	2055, 2840
BstSCI	CCNGG	8 cut(s)	125, 183, 492, 1170, 1461, 1830, 2121, 2243
BstSFI	CTRYAG	2 cut(s)	148, 2568
BstSLI	GKGCMC	1 cut(s)	2398
BstUI	CGCG	4 cut(s)	144, 2400, 2450, 2976
BstV2I	GAAGAC	3 cut(s)	156, 830, 1223
BstX2I	RGATCY	4 cut(s)	2029, 2281, 2756, 3005
BstXI	CCANNNNNNTGG	5 cut(s)	191, 397, 612, 1332, 2589
BstYI	RGATCY	4 cut(s)	2029, 2281, 2756, 3005
BsuI	GTATCC	2 cut(s)	1459, 2859
BsuRI	GGCC	3 cut(s)	183, 617, 1460
BtgI	CCRYGG	3 cut(s)	300, 1055, 1765
BtgZI	GCGATG	1 cut(s)	2465
BtsCI	GGATG	6 cut(s)	310, 592, 1275, 1370, 2131, 2578
BtsIMutI	CAGTG	2 cut(s)	736, 2064
BveI	ACCTGC	1 cut(s)	1138
Cac8I	GCNNGC	9 cut(s)	142, 528, 606, 2053, 2310, 2398, 2785, 2797, 2838
CaiI	CAGNNNCTG	3 cut(s)	526, 2189, 2671
CfoI	GCGC	4 cut(s)	532, 1410, 1985, 2265
Cfr10I	RCCGGY	1 cut(s)	2215
Cfr13I	GGNCC	3 cut(s)	615, 1459, 2592
CseI	GACGC	5 cut(s)	133, 597, 1816, 2456, 2842
Csp6I	GTAC	6 cut(s)	490, 1505, 2249, 2433, 2618, 2816
CspCI	CAANNNNNGTGG	4 cut(s)	715, 750, 2584, 2619
CviQI	GTAC	6 cut(s)	490, 1505, 2249, 2433, 2618, 2816
DdeI	CTNAG	7 cut(s)	434, 462, 971, 1704, 2230, 2870, 2990
DinI	GGCGCC	1 cut(s)	531
Eam1104I	CTCTTC	3 cut(s)	318, 830, 2408
EarI	CTCTTC	3 cut(s)	318, 830, 2408
EciI	GGCGGA	1 cut(s)	1146
Eco130I	CCWWGG	4 cut(s)	1055, 1430, 2500, 2602
Eco31I	GGTCTC	1 cut(s)	2893
Eco47I	GGWCC	1 cut(s)	2592
Eco57I	CTGAAG	6 cut(s)	342, 879, 995, 1605, 2088, 2648
EcoNI	CCTNNNNNAGG	1 cut(s)	1998
EcoO109I	RGGNCCY	1 cut(s)	1459
EcoRII	CCWGG	7 cut(s)	125, 183, 492, 1461, 1830, 2121, 2243
EcoT14I	CCWWGG	4 cut(s)	1055, 1430, 2500, 2602
EcoT22I	ATGCAT	2 cut(s)	702, 2057
EgeI	GGCGCC	1 cut(s)	531
EheI	GGCGCC	1 cut(s)	531
ErhI	CCWWGG	4 cut(s)	1055, 1430, 2500, 2602
FalI	AAGNNNNNCTT	4 cut(s)	882, 914, 2783, 2815
FaqI	GGGAC	3 cut(s)	187, 1860, 2183
FauNDI	CATATG	1 cut(s)	2109
FbaI	TGATCA	1 cut(s)	1138
FblI	GTMKAC	1 cut(s)	1965
FokI	GGATG	6 cut(s)	317, 599, 1282, 1357, 2138, 2565
FspAI	RTGCGCAY	1 cut(s)	2264
FspBI	CTAG	8 cut(s)	14, 944, 1431, 1533, 2313, 2481, 2501, 3025
FspI	TGCGCA	2 cut(s)	1984, 2264
GlaI	GCGC	4 cut(s)	531, 1409, 1984, 2264
GsaI	CCCAGC	1 cut(s)	645
GsuI	CTGGAG	4 cut(s)	137, 362, 1853, 2930
HaeII	RGCGCY	2 cut(s)	533, 1411
HaeIII	GGCC	3 cut(s)	183, 617, 1460
HapII	CCGG	5 cut(s)	674, 1172, 2090, 2216, 2969
HgaI	GACGC	5 cut(s)	133, 597, 1816, 2456, 2842
HhaI	GCGC	4 cut(s)	532, 1410, 1985, 2265
Hin1I	GRCGYC	3 cut(s)	530, 1827, 1849
Hin6I	GCGC	4 cut(s)	530, 1408, 1983, 2263
HinP1I	GCGC	4 cut(s)	530, 1408, 1983, 2263
HincII	GTYRAC	3 cut(s)	1561, 1918, 2851
HindII	GTYRAC	3 cut(s)	1561, 1918, 2851
HindIII	AAGCTT	3 cut(s)	56, 1007, 2174
HpaII	CCGG	5 cut(s)	674, 1172, 2090, 2216, 2969
HphI	GGTGA	7 cut(s)	364, 791, 2079, 2111, 2359, 2558, 2572
Hpy188I	TCNGA	9 cut(s)	243, 322, 435, 638, 871, 1813, 2165, 2993, 3005
Hpy188III	TCNNGA	7 cut(s)	116, 332, 788, 1064, 1757, 1997, 2232
Hpy99I	CGWCG	1 cut(s)	1854
HpyCH4III	ACNGT	4 cut(s)	666, 731, 1565, 2359
HpyCH4IV	ACGT	2 cut(s)	1849, 2847
HpyF3I	CTNAG	7 cut(s)	434, 462, 971, 1704, 2230, 2870, 2990
HpySE526I	ACGT	2 cut(s)	1849, 2847
Hsp92I	GRCGYC	3 cut(s)	530, 1827, 1849
HspAI	GCGC	4 cut(s)	530, 1408, 1983, 2263
KasI	GGCGCC	1 cut(s)	529
KpnI	GGTACC	1 cut(s)	2436
Ksp22I	TGATCA	1 cut(s)	1138
LguI	GCTCTTC	1 cut(s)	830
LweI	GCATC	8 cut(s)	206, 466, 577, 1141, 2260, 2442, 2474, 2653
MaeI	CTAG	8 cut(s)	14, 944, 1431, 1533, 2313, 2481, 2501, 3025
MaeII	ACGT	2 cut(s)	1849, 2847
MaeIII	GTNAC	7 cut(s)	245, 877, 982, 1645, 1942, 2380, 2843
MfeI	CAATTG	1 cut(s)	774
MflI	RGATCY	4 cut(s)	2029, 2281, 2756, 3005
MhlI	GDGCHC	2 cut(s)	2398, 3003
Mly113I	GGCGCC	1 cut(s)	530
MlyI	GAGTC	2 cut(s)	380, 1420
MmeI	TCCRAC	2 cut(s)	2321, 2901
Mph1103I	ATGCAT	2 cut(s)	702, 2057
MroXI	GAANNNNTTC	2 cut(s)	327, 1572
MslI	CAYNNNNRTG	3 cut(s)	2124, 2768, 2841
MspA1I	CMGCKG	1 cut(s)	2186
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	5 cut(s)	674, 1172, 2090, 2216, 2969
MspR9I	CCNGG	8 cut(s)	127, 185, 494, 1172, 1463, 1832, 2123, 2245
MunI	CAATTG	1 cut(s)	774
Mva1269I	GAATGC	3 cut(s)	429, 1175, 1250
MvaI	CCWGG	7 cut(s)	127, 185, 494, 1463, 1832, 2123, 2245
MvnI	CGCG	4 cut(s)	144, 2400, 2450, 2976
NarI	GGCGCC	1 cut(s)	530
NciI	CCSGG	1 cut(s)	1172
NcoI	CCATGG	1 cut(s)	1055
NdeI	CATATG	1 cut(s)	2109
NlaIV	GGNNCC	5 cut(s)	531, 1320, 1810, 2434, 2758
NmeAIII	GCCGAG	2 cut(s)	310, 625
NmuCI	GTSAC	3 cut(s)	877, 1645, 2843
NsbI	TGCGCA	2 cut(s)	1984, 2264
NsiI	ATGCAT	2 cut(s)	702, 2057
NspI	RCATGY	2 cut(s)	2055, 2840
NspV	TTCGAA	1 cut(s)	660
PacI	TTAATTAA	1 cut(s)	2325
PaeI	GCATGC	2 cut(s)	2055, 2840
PciSI	GCTCTTC	1 cut(s)	830
PctI	GAATGC	3 cut(s)	429, 1175, 1250
PdmI	GAANNNNTTC	2 cut(s)	327, 1572
PfeI	GAWTC	8 cut(s)	104, 238, 311, 328, 866, 1096, 2575, 2822
PflFI	GACNNNGTC	3 cut(s)	228, 1851, 2696
PflMI	CCANNNNNTGG	1 cut(s)	2044
PfoI	TCCNGGA	1 cut(s)	1830
Ple19I	CGATCG	1 cut(s)	2338
PleI	GAGTC	2 cut(s)	379, 1420
PluTI	GGCGCC	1 cut(s)	533
PpsI	GAGTC	2 cut(s)	379, 1420
PshBI	ATTAAT	3 cut(s)	2058, 2325, 2952
Psp6I	CCWGG	7 cut(s)	125, 183, 492, 1461, 1830, 2121, 2243
PspFI	CCCAGC	1 cut(s)	641
PspGI	CCWGG	7 cut(s)	125, 183, 492, 1461, 1830, 2121, 2243
PspN4I	GGNNCC	5 cut(s)	531, 1320, 1810, 2434, 2758
PspPI	GGNCC	3 cut(s)	615, 1459, 2592
PstI	CTGCAG	2 cut(s)	152, 2572
PstNI	CAGNNNCTG	3 cut(s)	526, 2189, 2671
PsuI	RGATCY	4 cut(s)	2029, 2281, 2756, 3005
PsyI	GACNNNGTC	3 cut(s)	228, 1851, 2696
PvuI	CGATCG	1 cut(s)	2338
PvuII	CAGCTG	1 cut(s)	2186
RsaI	GTAC	6 cut(s)	491, 1506, 2250, 2434, 2619, 2817
RsaNI	GTAC	6 cut(s)	490, 1505, 2249, 2433, 2618, 2816
RseI	CAYNNNNRTG	3 cut(s)	2124, 2768, 2841
SapI	GCTCTTC	1 cut(s)	830
Sau96I	GGNCC	3 cut(s)	615, 1459, 2592
SchI	GAGTC	2 cut(s)	380, 1420
ScrFI	CCNGG	8 cut(s)	127, 185, 494, 1172, 1463, 1832, 2123, 2245
SduI	GDGCHC	2 cut(s)	2398, 3003
SfaNI	GCATC	8 cut(s)	206, 466, 577, 1141, 2260, 2442, 2474, 2653
SfcI	CTRYAG	2 cut(s)	148, 2568
SfoI	GGCGCC	1 cut(s)	531
SfuI	TTCGAA	1 cut(s)	660
SinI	GGWCC	1 cut(s)	2592
SmiMI	CAYNNNNRTG	3 cut(s)	2124, 2768, 2841
SmlI	CTYRAG	3 cut(s)	89, 788, 1190
SmoI	CTYRAG	3 cut(s)	89, 788, 1190
SphI	GCATGC	2 cut(s)	2055, 2840
SsiI	CCGC	9 cut(s)	479, 597, 671, 1157, 1748, 2024, 2463, 2629, 2976
SspDI	GGCGCC	1 cut(s)	529
SspMI	CTAG	8 cut(s)	14, 944, 1431, 1533, 2313, 2481, 2501, 3025
StyD4I	CCNGG	8 cut(s)	125, 183, 492, 1170, 1461, 1830, 2121, 2243
StyI	CCWWGG	4 cut(s)	1055, 1430, 2500, 2602
TaaI	ACNGT	4 cut(s)	666, 731, 1565, 2359
TaiI	ACGT	2 cut(s)	1852, 2850
TaqI	TCGA	9 cut(s)	107, 660, 1090, 1424, 1714, 1939, 2253, 2334, 2587
TatI	WGTACW	2 cut(s)	2617, 2815
TauI	GCSGC	4 cut(s)	482, 599, 673, 2465
TfiI	GAWTC	8 cut(s)	104, 238, 311, 328, 866, 1096, 2575, 2822
TscAI	CASTG	2 cut(s)	736, 2071
TseFI	GTSAC	3 cut(s)	877, 1645, 2843
Tsp45I	GTSAC	3 cut(s)	877, 1645, 2843
TspDTI	ATGAA	6 cut(s)	900, 1736, 2121, 2292, 2814, 2873
TspGWI	ACGGA	4 cut(s)	317, 642, 1754, 1967
TspRI	CASTG	2 cut(s)	736, 2071
Tth111I	GACNNNGTC	3 cut(s)	228, 1851, 2696
Van91I	CCANNNNNTGG	1 cut(s)	2044
Vha464I	CTTAAG	1 cut(s)	1190
VneI	GTGCAC	1 cut(s)	2394
VpaK11BI	GGWCC	1 cut(s)	2592
VspI	ATTAAT	3 cut(s)	2058, 2325, 2952
XagI	CCTNNNNNAGG	1 cut(s)	1998
XapI	RAATTY	5 cut(s)	535, 1897, 1971, 2803, 2914
XceI	RCATGY	2 cut(s)	2055, 2840
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2750
XmaJI	CCTAGG	2 cut(s)	1430, 2500
XmiI	GTMKAC	1 cut(s)	1965
XmnI	GAANNNNTTC	2 cut(s)	327, 1572
XspI	CTAG	8 cut(s)	14, 944, 1431, 1533, 2313, 2481, 2501, 3025
ZraI	GACGTC	1 cut(s)	1850
Zsp2I	ATGCAT	2 cut(s)	702, 2057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.