Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007623

Physical Location & Seq

Genomic Coordinates Reverse (-)

3817655 .. 3824220

Gene Structure 6566 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1054000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGTGGAGAAACTTCGCCAAAAAGGTTGCTTCCAGAAAGGCAAATCCTTCCTCACAGTTTCATTTGGGTTCTGCTACTTCTTCTTCAGTGAAATTTTCTCAAGAATCCAGCTTTATCAAGAAAACCAGGCATTTCGAGGCCCGGAATTGTGTAAACAGTCGGTTATTGGTGATGGGTTTTCGAAAAATTGGCGATACAGCTTCCCAGAAGGACGAATTGGGCAGATGCAGTTTGCTGAATCCGAGTTACTTTCATGGCGTTTCTGGGTTTTGTAGCTATGCCAGAGGCTATGCCAGTGCTGCCGAGGCCATTGCGTCGACGGATGGGGATGAGGAGTCGTCGGGGTCTGAAGAAATTCAGGAATTGTTGGAGGATTTGATCAGAGAGAACAACAAGGTGGAGTCCCATTTCAAGCAGCCGAAGAAAGTGGTTGCTGGTATAGGAGTAGGAAAGTACAATGTCCTCCGGAAGAGGCAAATAAAGCTGGAGACCGAGGCGTGGGAAGAGGCGGCGAACGAGTACCAAGAGCTTTTAGTGGACATGTGCGAGCAGAAGCTTGCACCCAATTTGCCATACATTAAGTCATTGTTTCTTGGTTGGTTTGAGCCTTTACGAAACGCCATTGCTGAGGACCAAGAGGCCTGCAGGCAACCGCACAATAGAGTCAGTCATGCTCCGTATATTGATCTCTTACCGGCCGATAAGATGGCTGTTATTACAATGCACAAGTTGATGGGGCTGTTGATGACAAATAATGGCGGAGTCGGGAGTGTCAGGGTAGTCCAAGCTGCTTGTGCGATAGGTGAAGCCATTGAGAATGAGGTTAGGATACACAGGTTTTTGGATAAGACAAAGAAGAAGAAGAACCCTACTGACAAGAAGCCTGAAGATGAATCTGATCCTGTGATCATTGAACAAGAGCAACTGTCAATCAGCGAGCAAGAGAAACTGACGAAAGAACAGGAGAGACTTAGGAAAAAGGTTAACAAACTGATAAAAAAGCAGAAAATGAAGCAAGTAAGGGAGATTGTGAAGGAACAAGAAGACTTAAAGCCATGGGGTCAGGAAGCCCATGTTAAGGTTGGCTGTCGATTGATTCAGTTGTTGATGGACACAGCTTATATACAACCTCCAGTTGATCAATCAGGAGATGATCCACTTGATATACGCCCCGCATTTGTACATGTCCTTAAGACTATCACTAAAGACACACAGAAGACTAGTAGGAGATATGGTGTTATAGAATGCGACCCATTAGTTCGCAAAGGCATGGAGAAATCTGCAAGGCACATGGTCATTCCTTATATGCCTATGTTGGTACCCCCTATCAACTGGACAGGGTATGACAGAGGAGCATATTTATTCTTACCATCCTATGTTATGCGAACTCATGGAGCGAAACAACAACGCCAAGTTGTTAAAAGGACTCCTAGGATGCAATTAGAACCTGTTTTTGAGGCCCTGGATACTCTTGGAAGTACCAAATGGAGGGTGAACAAACGGGTACTTGGTGTAATAGACAGGATATGGGCTAGTGGAGGCCGTCTTGCTGACTTGGTTGACCGTGAAGATGTTCCTTTGCCTGAAGAACCAGACACAGAAGATGAGACAGAAATAAAAAAATGGAAGTGGAAAGTTAAAGCTGCAAAGAAGGAGAATAGTGAGAGACACTCACAGCGGTGTGACATAGAACTCAAACTTGCTGTATCTCGAAAAATGAAGGATGAAGAAGGCTTCTACTACCCGCACAACCTTGATTTCCGTGGTCGTGCTTACCCCATGCACCCATATCTAAACCATCTTGGTTCTGATATGTGCCGAGGCATCCTGGAGTTTGCAGAGGGACGGCGTCTTGGAAAGTCAGGCTTACAGTGGTTGAAGATACATTTGGCAAATTTATATGCTGGTGGCGTGGACAAACTCTCCTTTGATGAGCGAGTAGCGTTTACTGAGAATCATGTGGATGAAATTTTTGATTCGGCAGTCAGACCTCTTGAAGGGAGGCGCTGGTGGTTGGGTGCTGAGGATCCTTTCCAATGCTTGGCAGCATGTATTAATCTCTATGAAGCATTGAGAAGCCCCTCTCCAGAGACTACCATTTCATATATGCCTGTCCACCAGGATGGTTCATGTAATGGTTTACAACATTATGCTGCTCTTGGAAGGGACAAGCTTGGAGCTGCTGCTGTTAATCTTGTGGGGGGAGAGAAACCTGCAGATGTTTACTCAGGAATTGCCACCAGGGTACTCGATATAATGCGGAGCGATGCAGAGAAAGATCCTGCCACTAATCCAAATGCATTGCATGCTAGGCTGTTAATTAATCAGGTGGATCGTAAATTGGTGAAGCAAACAGTTATGACGTCTGTATATGGTGTTACTTATGTTGGTGCACGTGATCAAATTAAGAGGAGATTAAAAGAGCGTAATAACATAGCTGATGACTCGGCGCTTTTTGCTGCATCTTGCTATGCTGCAAGGACCACCTTGACTGCACTAGGCGAGATGTTTGAAGCTGCAAGAAGTATTATGAGTTGGCTCGGTGAATGTGCAAAGGTGATAGCTTCGGAGAATCATCCTGTTCAGTGGACCACTCCCCTTGGACTTCCCGTTGTACAACCCTACCGACAATTAGGAAGGCATCTGATTAAGACTTCCCTTCAGGTGTTGACGTTACAAAGAGAAACTGACAAGGTCATGGTTAAACGGCAGAGGACAGCCTTTCCCCCAAATTTTGTGCACTCCCTTGACGGTTCCCACATGATGATGACTGCTGTTGCCTGCAAGAAAGCAGGCTTAAATTTTGCAGGAGTGCACGATTCATATTGGACGCATGCGTGTGATGTTGACGAAATGAATAGGATACTAAGGGAGAAGTTTGTTGAACTCTACGACGCACCGATACTGGAGAATTTATTGGAGGGCTTTCAAGAGTCTTTCCCCAACATGACTTTTCCCCCTTTGCCAGATCGTGGAGACTTCGATCTCAGAGATGTTCTTGAATCTCCCTATTTCTTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

1008

Amino Acids

114.26

Weight (kDa)

8.56

Isoelectric Point (pI)

48.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	158 - 493	8.3e-86	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	617 - 982	1.6e-157	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68990 AT1G68990
fragaria_vesca	FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970
malus_domestica	MD13G1055100.v1.1 MD16G1054000.v1.1
prunus_persica	Prupe.1G295700_v2.0.a1 Prupe.1G295700_v2.0.a1
pyrus_communis	pycom13g04880 pycom16g04700
rosa_chinensis	RchiOBHm_Chr4g0437111
rosa_laevigata	RLG00000006467
rosa_multiflora	Rmu_co8109606.1_g000001 Rmu_ssc0000006.1_g000008
rosa_roxburghii	Rroxscaffold_5G00378180
rosa_rugosa	Rorug04G0298100
rosa_samantha	Rh4AG352900 Rh4BG362400 Rh4CG376600 Rh4DG356400
rosa_wichuraiana	Rw4G030900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2366
Acc36I	ACCTGC	1 cut(s)	2221
Acc65I	GGTACC	1 cut(s)	1318
AccB1I	GGYRCC	1 cut(s)	1318
AccB7I	CCANNNNNTGG	2 cut(s)	2041, 2972
AccI	GTMKAC	1 cut(s)	317
AccIII	TCCGGA	1 cut(s)	465
AciI	CCGC	7 cut(s)	509, 653, 759, 1173, 1678, 1745, 2260
AclWI	GGATC	6 cut(s)	893, 1148, 2021, 2034, 2273, 2340
AcoI	YGGCCR	1 cut(s)	696
AcsI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
AcuI	CTGAAG	5 cut(s)	69, 369, 906, 1605, 2645
AcvI	CACGTG	1 cut(s)	2396
AcyI	GRCGYC	2 cut(s)	1849, 2363
AfaI	GTAC	8 cut(s)	455, 521, 1182, 1320, 1480, 1506, 2247, 2616
AfiI	CCNNNNNNNGG	6 cut(s)	498, 1078, 1488, 1997, 2041, 2972
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	2 cut(s)	540, 1183
AgsI	TTSAA	9 cut(s)	412, 914, 1879, 1997, 2513, 2885, 2930, 3002, 3019
AhlI	ACTAGT	1 cut(s)	1220
AjnI	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
AjuI	GAANNNNNNNTTGG	4 cut(s)	200, 232, 1871, 1903
AleI	CACNNNNGTG	1 cut(s)	1678
Alw21I	GWGCWC	3 cut(s)	2395, 2742, 2817
Alw26I	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
Alw44I	GTGCAC	3 cut(s)	2391, 2738, 2813
AlwI	GGATC	6 cut(s)	893, 1148, 2021, 2034, 2273, 2340
Aor13HI	TCCGGA	1 cut(s)	465
AoxI	GGCC	6 cut(s)	138, 306, 639, 696, 1458, 1540
ApaLI	GTGCAC	3 cut(s)	2391, 2738, 2813
ApoI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
AseI	ATTAAT	2 cut(s)	2055, 2322
Asp700I	GAANNNNTTC	2 cut(s)	354, 1572
Asp718I	GGTACC	1 cut(s)	1318
AspA2I	CCTAGG	1 cut(s)	1430
AspLEI	GCGC	2 cut(s)	2007, 2452
AspS9I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
AsuC2I	CCSGG	1 cut(s)	142
AsuHPI	GGTGA	6 cut(s)	181, 815, 1504, 2356, 2555, 2569
AsuII	TTCGAA	1 cut(s)	181
AvaII	GGWCC	3 cut(s)	631, 2481, 2589
AvrII	CCTAGG	1 cut(s)	1430
BaeGI	GKGCMC	3 cut(s)	2395, 2742, 2817
BaeI	ACNNNNGTAYC	2 cut(s)	821, 854
BamHI	GGATCC	1 cut(s)	2026
BanI	GGYRCC	1 cut(s)	1318
BbrPI	CACGTG	1 cut(s)	2396
BbsI	GAAGAC	2 cut(s)	1050, 1223
Bbv12I	GWGCWC	3 cut(s)	2395, 2742, 2817
BbvCI	CCTCAGC	2 cut(s)	627, 2022
BccI	CCATC	8 cut(s)	166, 317, 700, 727, 1102, 1378, 1806, 2117
BceAI	ACGGC	3 cut(s)	1527, 1862, 2723
BcgI	CGANNNNNNTGC	2 cut(s)	293, 327
BciT130I	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BciVI	GTATCC	3 cut(s)	822, 1459, 2856
BclI	TGATCA	4 cut(s)	378, 906, 1138, 2398
BcnI	CCSGG	1 cut(s)	142
BcoDI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BcuI	ACTAGT	1 cut(s)	1220
BfaI	CTAG	6 cut(s)	1221, 1431, 1533, 2310, 2498, 3022
BfmI	CTRYAG	2 cut(s)	643, 2214
BfoI	RGCGCY	2 cut(s)	2008, 2453
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	1 cut(s)	2221
BfuI	GTATCC	3 cut(s)	822, 1459, 2856
BlnI	CCTAGG	1 cut(s)	1430
Bme1390I	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
Bme18I	GGWCC	3 cut(s)	631, 2481, 2589
BmgT120I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
BmiI	GGNNCC	3 cut(s)	1320, 2028, 2755
BmrFI	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
BmsI	GCATC	6 cut(s)	215, 1425, 1833, 2257, 2471, 2650
BpiI	GAAGAC	2 cut(s)	1050, 1223
BplI	GAGNNNNNCTC	2 cut(s)	1655, 1687
BpmI	CTGGAG	5 cut(s)	506, 1116, 1850, 2070, 2927
Bpu10I	CCTNAGC	2 cut(s)	627, 2022
Bpu14I	TTCGAA	1 cut(s)	181
BpuEI	CTTGAG	1 cut(s)	84
BpuMI	CCSGG	1 cut(s)	142
BsaAI	YACGTR	1 cut(s)	2396
BsaHI	GRCGYC	2 cut(s)	1849, 2363
BsaI	GGTCTC	1 cut(s)	482
BsaJI	CCNNGG	9 cut(s)	303, 492, 1055, 1430, 1461, 1762, 1819, 2241, 2599
BsaWI	WCCGGW	1 cut(s)	465
BsaXI	ACNNNNNCTCC	4 cut(s)	1219, 1249, 1907, 1937
Bsc4I	CCNNNNNNNGG	6 cut(s)	498, 1078, 1488, 1997, 2041, 2972
Bse118I	RCCGGY	1 cut(s)	694
Bse1I	ACTGG	4 cut(s)	294, 1133, 1337, 2910
Bse3DI	GCAATG	3 cut(s)	309, 621, 2300
BseAI	TCCGGA	1 cut(s)	465
BseBI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BseDI	CCNNGG	9 cut(s)	303, 492, 1055, 1430, 1461, 1762, 1819, 2241, 2599
BseGI	GGATG	9 cut(s)	328, 334, 1370, 1440, 1729, 1824, 1969, 2128, 2575
BseLI	CCNNNNNNNGG	6 cut(s)	498, 1078, 1488, 1997, 2041, 2972
BseMI	GCAATG	3 cut(s)	309, 621, 2300
BseMII	CTCAG	5 cut(s)	618, 1941, 2013, 2241, 3001
BseNI	ACTGG	4 cut(s)	294, 1133, 1337, 2910
BseRI	GAGGAG	3 cut(s)	347, 1365, 2425
BseSI	GKGCMC	3 cut(s)	2395, 2742, 2817
BseX3I	CGGCCG	1 cut(s)	696
BsgI	GTGCAG	1 cut(s)	2478
Bsh1285I	CGRYCG	1 cut(s)	699
BshFI	GGCC	6 cut(s)	140, 308, 641, 698, 1460, 1542
BshNI	GGYRCC	1 cut(s)	1318
BsiEI	CGRYCG	1 cut(s)	699
BsiHKAI	GWGCWC	3 cut(s)	2395, 2742, 2817
BsiSI	CCGG	3 cut(s)	142, 466, 695
BslFI	GGGAC	3 cut(s)	388, 1857, 2180
BslI	CCNNNNNNNGG	6 cut(s)	498, 1078, 1488, 1997, 2041, 2972
BsmAI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BsmFI	GGGAC	3 cut(s)	388, 1857, 2180
BsmI	GAATGC	1 cut(s)	1250
BsnI	GGCC	6 cut(s)	140, 308, 641, 698, 1460, 1542
Bso31I	GGTCTC	1 cut(s)	482
Bsp119I	TTCGAA	1 cut(s)	181
Bsp1286I	GDGCHC	3 cut(s)	2395, 2742, 2817
Bsp13I	TCCGGA	1 cut(s)	465
Bsp1407I	TGTACA	2 cut(s)	1180, 2614
Bsp19I	CCATGG	1 cut(s)	1055
BspACI	CCGC	7 cut(s)	509, 653, 759, 1173, 1678, 1745, 2260
BspANI	GGCC	6 cut(s)	140, 308, 641, 698, 1460, 1542
BspCNI	CTCAG	5 cut(s)	619, 1942, 2014, 2240, 3000
BspEI	TCCGGA	1 cut(s)	465
BspLI	GGNNCC	3 cut(s)	1320, 2028, 2755
BspMAI	CTGCAG	2 cut(s)	647, 2218
BspMI	ACCTGC	1 cut(s)	2221
BspPI	GGATC	6 cut(s)	893, 1148, 2021, 2034, 2273, 2340
BspT104I	TTCGAA	1 cut(s)	181
BspT107I	GGYRCC	1 cut(s)	1318
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	482
BsrDI	GCAATG	3 cut(s)	309, 621, 2300
BsrFI	RCCGGY	1 cut(s)	694
BsrGI	TGTACA	2 cut(s)	1180, 2614
BsrI	ACTGG	4 cut(s)	294, 1133, 1337, 2910
BssAI	RCCGGY	1 cut(s)	694
BssECI	CCNNGG	9 cut(s)	303, 492, 1055, 1430, 1461, 1762, 1819, 2241, 2599
BssNI	GRCGYC	2 cut(s)	1849, 2363
BssT1I	CCWWGG	3 cut(s)	1055, 1430, 2599
Bst2UI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
Bst4CI	ACNGT	7 cut(s)	57, 158, 927, 1565, 1872, 2356, 2753
Bst6I	CTCTTC	2 cut(s)	464, 498
BstACI	GRCGYC	2 cut(s)	1849, 2363
BstAFI	CTTAAG	1 cut(s)	1190
BstAPI	GCANNNNNTGC	1 cut(s)	2306
BstAUI	TGTACA	2 cut(s)	1180, 2614
BstBAI	YACGTR	1 cut(s)	2396
BstBI	TTCGAA	1 cut(s)	181
BstC8I	GCNNGC	9 cut(s)	548, 558, 643, 647, 938, 2307, 2782, 2794, 2835
BstDEI	CTNAG	7 cut(s)	627, 971, 1950, 2022, 2227, 2867, 2987
BstDSI	CCRYGG	2 cut(s)	1055, 1762
BstENI	CCTNNNNNAGG	1 cut(s)	1995
BstF5I	GGATG	9 cut(s)	328, 334, 1370, 1440, 1729, 1824, 1969, 2128, 2575
BstH2I	RGCGCY	2 cut(s)	2008, 2453
BstHHI	GCGC	2 cut(s)	2007, 2452
BstMAI	GTCTC	6 cut(s)	482, 961, 1601, 1660, 2084, 2970
BstMCI	CGRYCG	1 cut(s)	699
BstMWI	GCNNNNNNNGC	7 cut(s)	299, 305, 481, 794, 2306, 2311, 2456
BstNI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
BstNSI	RCATGY	5 cut(s)	544, 1187, 2052, 2309, 2837
BstSCI	CCNGG	6 cut(s)	125, 140, 1461, 1827, 2118, 2240
BstSFI	CTRYAG	2 cut(s)	643, 2214
BstSLI	GKGCMC	3 cut(s)	2395, 2742, 2817
BstV2I	GAAGAC	2 cut(s)	1050, 1223
BstX2I	RGATCY	2 cut(s)	2026, 2278
BstXI	CCANNNNNNTGG	1 cut(s)	2123
BstYI	RGATCY	2 cut(s)	2026, 2278
BstZI	CGGCCG	1 cut(s)	696
BsuI	GTATCC	3 cut(s)	822, 1459, 2856
BsuRI	GGCC	6 cut(s)	140, 308, 641, 698, 1460, 1542
BtgI	CCRYGG	2 cut(s)	1055, 1762
BtgZI	GCGATG	1 cut(s)	2280
BtsCI	GGATG	9 cut(s)	328, 334, 1370, 1440, 1729, 1824, 1969, 2128, 2575
BtsIMutI	CAGTG	4 cut(s)	93, 301, 1877, 2591
BveI	ACCTGC	1 cut(s)	2221
Cac8I	GCNNGC	9 cut(s)	548, 558, 643, 647, 938, 2307, 2782, 2794, 2835
CfoI	GCGC	2 cut(s)	2007, 2452
Cfr10I	RCCGGY	1 cut(s)	694
Cfr13I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
CseI	GACGC	4 cut(s)	303, 1838, 2839, 2903
Csp6I	GTAC	8 cut(s)	454, 520, 1181, 1319, 1479, 1505, 2246, 2615
CspCI	CAANNNNNGTGG	2 cut(s)	2581, 2616
CviQI	GTAC	8 cut(s)	454, 520, 1181, 1319, 1479, 1505, 2246, 2615
DdeI	CTNAG	7 cut(s)	627, 971, 1950, 2022, 2227, 2867, 2987
EaeI	YGGCCR	1 cut(s)	696
EagI	CGGCCG	1 cut(s)	696
Eam1104I	CTCTTC	2 cut(s)	464, 498
EarI	CTCTTC	2 cut(s)	464, 498
EciI	GGCGGA	1 cut(s)	774
EclXI	CGGCCG	1 cut(s)	696
Eco130I	CCWWGG	3 cut(s)	1055, 1430, 2599
Eco147I	AGGCCT	1 cut(s)	641
Eco31I	GGTCTC	1 cut(s)	482
Eco47I	GGWCC	3 cut(s)	631, 2481, 2589
Eco52I	CGGCCG	1 cut(s)	696
Eco57I	CTGAAG	5 cut(s)	69, 369, 906, 1605, 2645
Eco72I	CACGTG	1 cut(s)	2396
EcoNI	CCTNNNNNAGG	1 cut(s)	1995
EcoO109I	RGGNCCY	1 cut(s)	1459
EcoRII	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
EcoT14I	CCWWGG	3 cut(s)	1055, 1430, 2599
EcoT22I	ATGCAT	1 cut(s)	2302
ErhI	CCWWGG	3 cut(s)	1055, 1430, 2599
FalI	AAGNNNNNCTT	2 cut(s)	1850, 1882
FaqI	GGGAC	3 cut(s)	388, 1857, 2180
FauI	CCCGC	2 cut(s)	1180, 1752
FbaI	TGATCA	4 cut(s)	378, 906, 1138, 2398
FblI	GTMKAC	1 cut(s)	317
FokI	GGATG	9 cut(s)	335, 341, 1357, 1447, 1736, 1811, 1976, 2135, 2562
FspBI	CTAG	6 cut(s)	1221, 1431, 1533, 2310, 2498, 3022
GlaI	GCGC	2 cut(s)	2006, 2451
GsuI	CTGGAG	5 cut(s)	506, 1116, 1850, 2070, 2927
HaeII	RGCGCY	2 cut(s)	2008, 2453
HaeIII	GGCC	6 cut(s)	140, 308, 641, 698, 1460, 1542
HapII	CCGG	3 cut(s)	142, 466, 695
HgaI	GACGC	4 cut(s)	303, 1838, 2839, 2903
HhaI	GCGC	2 cut(s)	2007, 2452
Hin1I	GRCGYC	2 cut(s)	1849, 2363
Hin6I	GCGC	2 cut(s)	2005, 2450
HinP1I	GCGC	2 cut(s)	2005, 2450
HincII	GTYRAC	5 cut(s)	318, 985, 1561, 2670, 2848
HindII	GTYRAC	5 cut(s)	318, 985, 1561, 2670, 2848
HindIII	AAGCTT	2 cut(s)	554, 2171
HpaI	GTTAAC	1 cut(s)	985
HpaII	CCGG	3 cut(s)	142, 466, 695
HphI	GGTGA	6 cut(s)	181, 815, 1504, 2356, 2555, 2569
Hpy188I	TCNGA	9 cut(s)	243, 349, 383, 898, 1810, 1988, 2569, 2646, 2990
Hpy99I	CGWCG	4 cut(s)	319, 322, 343, 2897
HpyCH4III	ACNGT	7 cut(s)	57, 158, 927, 1565, 1872, 2356, 2753
HpyCH4IV	ACGT	3 cut(s)	2363, 2395, 2672
HpyF10VI	GCNNNNNNNGC	7 cut(s)	299, 305, 481, 794, 2306, 2311, 2456
HpyF3I	CTNAG	7 cut(s)	627, 971, 1950, 2022, 2227, 2867, 2987
HpySE526I	ACGT	3 cut(s)	2363, 2395, 2672
Hsp92I	GRCGYC	2 cut(s)	1849, 2363
HspAI	GCGC	2 cut(s)	2005, 2450
Kpn2I	TCCGGA	1 cut(s)	465
KpnI	GGTACC	1 cut(s)	1322
Ksp22I	TGATCA	4 cut(s)	378, 906, 1138, 2398
KspAI	GTTAAC	1 cut(s)	985
LmnI	GCTCC	5 cut(s)	679, 1352, 1394, 2177, 2262
LweI	GCATC	6 cut(s)	215, 1425, 1833, 2257, 2471, 2650
MaeI	CTAG	6 cut(s)	1221, 1431, 1533, 2310, 2498, 3022
MaeII	ACGT	3 cut(s)	2363, 2395, 2672
MaeIII	GTNAC	4 cut(s)	245, 1682, 2377, 2673
MflI	RGATCY	2 cut(s)	2026, 2278
MhlI	GDGCHC	3 cut(s)	2395, 2742, 2817
MlyI	GAGTC	7 cut(s)	344, 410, 672, 771, 1420, 2438, 2942
MmeI	TCCRAC	1 cut(s)	348
Mph1103I	ATGCAT	1 cut(s)	2302
MroI	TCCGGA	1 cut(s)	465
MroXI	GAANNNNTTC	2 cut(s)	354, 1572
MslI	CAYNNNNRTG	5 cut(s)	1678, 1962, 2121, 2765, 2838
MspA1I	CMGCKG	1 cut(s)	1678
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	3 cut(s)	142, 466, 695
MspR9I	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
Mva1269I	GAATGC	1 cut(s)	1250
MvaI	CCWGG	5 cut(s)	127, 1463, 1829, 2120, 2242
MwoI	GCNNNNNNNGC	7 cut(s)	299, 305, 481, 794, 2306, 2311, 2456
NciI	CCSGG	1 cut(s)	142
NcoI	CCATGG	1 cut(s)	1055
NlaIV	GGNNCC	3 cut(s)	1320, 2028, 2755
NmeAIII	GCCGAG	3 cut(s)	328, 1844, 2426
NmuCI	GTSAC	1 cut(s)	1682
NsiI	ATGCAT	1 cut(s)	2302
NspI	RCATGY	5 cut(s)	544, 1187, 2052, 2309, 2837
NspV	TTCGAA	1 cut(s)	181
OliI	CACNNNNGTG	1 cut(s)	1678
PacI	TTAATTAA	1 cut(s)	2322
PaeI	GCATGC	2 cut(s)	2309, 2837
PceI	AGGCCT	1 cut(s)	641
PciI	ACATGT	2 cut(s)	540, 1183
PctI	GAATGC	1 cut(s)	1250
PdmI	GAANNNNTTC	2 cut(s)	354, 1572
PfeI	GAWTC	9 cut(s)	104, 238, 893, 1096, 1954, 1976, 2572, 2819, 3002
PflFI	GACNNNGTC	2 cut(s)	1848, 2693
PflMI	CCANNNNNTGG	2 cut(s)	2041, 2972
PfoI	TCCNGGA	1 cut(s)	1827
PleI	GAGTC	7 cut(s)	343, 409, 671, 770, 1420, 2438, 2941
PmaCI	CACGTG	1 cut(s)	2396
PmlI	CACGTG	1 cut(s)	2396
PpsI	GAGTC	7 cut(s)	343, 409, 671, 770, 1420, 2438, 2941
Ppu21I	YACGTR	1 cut(s)	2396
PscI	ACATGT	2 cut(s)	540, 1183
PshBI	ATTAAT	2 cut(s)	2055, 2322
Psp6I	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
PspCI	CACGTG	1 cut(s)	2396
PspGI	CCWGG	5 cut(s)	125, 1461, 1827, 2118, 2240
PspN4I	GGNNCC	3 cut(s)	1320, 2028, 2755
PspPI	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
PstI	CTGCAG	2 cut(s)	647, 2218
PsuI	RGATCY	2 cut(s)	2026, 2278
PsyI	GACNNNGTC	2 cut(s)	1848, 2693
RsaI	GTAC	8 cut(s)	455, 521, 1182, 1320, 1480, 1506, 2247, 2616
RsaNI	GTAC	8 cut(s)	454, 520, 1181, 1319, 1479, 1505, 2246, 2615
RseI	CAYNNNNRTG	5 cut(s)	1678, 1962, 2121, 2765, 2838
SalI	GTCGAC	1 cut(s)	316
Sau96I	GGNCC	5 cut(s)	139, 631, 1459, 2481, 2589
SbfI	CCTGCAGG	1 cut(s)	647
SchI	GAGTC	7 cut(s)	344, 410, 672, 771, 1420, 2438, 2942
ScrFI	CCNGG	6 cut(s)	127, 142, 1463, 1829, 2120, 2242
SdaI	CCTGCAGG	1 cut(s)	647
SduI	GDGCHC	3 cut(s)	2395, 2742, 2817
SfaNI	GCATC	6 cut(s)	215, 1425, 1833, 2257, 2471, 2650
SfcI	CTRYAG	2 cut(s)	643, 2214
SfuI	TTCGAA	1 cut(s)	181
SgrDI	CGTCGACG	1 cut(s)	316
SinI	GGWCC	3 cut(s)	631, 2481, 2589
SmiMI	CAYNNNNRTG	5 cut(s)	1678, 1962, 2121, 2765, 2838
SmlI	CTYRAG	2 cut(s)	99, 1190
SmoI	CTYRAG	2 cut(s)	99, 1190
SpeI	ACTAGT	1 cut(s)	1220
SphI	GCATGC	2 cut(s)	2309, 2837
Sse8387I	CCTGCAGG	1 cut(s)	647
SseBI	AGGCCT	1 cut(s)	641
SsiI	CCGC	7 cut(s)	509, 653, 759, 1173, 1678, 1745, 2260
SspMI	CTAG	6 cut(s)	1221, 1431, 1533, 2310, 2498, 3022
StuI	AGGCCT	1 cut(s)	641
StyD4I	CCNGG	6 cut(s)	125, 140, 1461, 1827, 2118, 2240
StyI	CCWWGG	3 cut(s)	1055, 1430, 2599
TaaI	ACNGT	7 cut(s)	57, 158, 927, 1565, 1872, 2356, 2753
TaiI	ACGT	3 cut(s)	2366, 2398, 2675
TaqI	TCGA	7 cut(s)	135, 181, 317, 1090, 1711, 2250, 2982
TaqII	GACCGA	1 cut(s)	506
TatI	WGTACW	3 cut(s)	453, 1180, 2614
TauI	GCSGC	1 cut(s)	512
TfiI	GAWTC	9 cut(s)	104, 238, 893, 1096, 1954, 1976, 2572, 2819, 3002
TscAI	CASTG	4 cut(s)	93, 301, 1877, 2591
TseFI	GTSAC	1 cut(s)	1682
Tsp45I	GTSAC	1 cut(s)	1682
TspGWI	ACGGA	3 cut(s)	335, 666, 1751
TspRI	CASTG	4 cut(s)	93, 301, 1877, 2591
Tth111I	GACNNNGTC	2 cut(s)	1848, 2693
Van91I	CCANNNNNTGG	2 cut(s)	2041, 2972
Vha464I	CTTAAG	1 cut(s)	1190
VneI	GTGCAC	3 cut(s)	2391, 2738, 2813
VpaK11BI	GGWCC	3 cut(s)	631, 2481, 2589
VspI	ATTAAT	2 cut(s)	2055, 2322
XagI	CCTNNNNNAGG	1 cut(s)	1995
XapI	RAATTY	7 cut(s)	92, 354, 1894, 1968, 2731, 2800, 2911
XceI	RCATGY	5 cut(s)	544, 1187, 2052, 2309, 2837
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1079
XmaJI	CCTAGG	1 cut(s)	1430
XmiI	GTMKAC	1 cut(s)	317
XmnI	GAANNNNTTC	2 cut(s)	354, 1572
XspI	CTAG	6 cut(s)	1221, 1431, 1533, 2310, 2498, 3022
ZraI	GACGTC	1 cut(s)	2364
Zsp2I	ATGCAT	1 cut(s)	2302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.