Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0007623

Physical Location & Seq

Genomic Coordinates Reverse (-)

57703549 .. 57711666

Gene Structure 8118 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G030900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGTGGAGAACCCTAGCCAAGCAAGCTGCTTCTTCTTCAACAAAGACCAAGCTTTTCTCTCCCTCTCCATTCAGCTCTGCTCTGAAACTTGCTCAAGAATCGAGCTTTCTGGAGAAAAGCAGGCATTTTGAGGCTCGCGTCTGCAGAAATCATGGGTCTTCGTTAATGGGTTTAGGCCAGGCTGGTGGTGCATCGTCCCAGAAAGACGGATTGGGCAGACCCAGTTTGCTGAACCCGAGTTACCCTCGTGGGTTTGGGAGATGGTATGCTACTTCTGCCGAGGCTATTGCTTCCACGGATGAGGAATCTGGGTCTGAAGAGATTCAGGAAATGCTGGAGGATTTGGTGAGGGAGAACAAGGTGGACTCGCATTTGAGGCAGCCCAAGAGGTTGGTTAATGGAATGGGAGCAGGAAAGTACGGCATTCTGAGAAAGAGGCAGTTGAAAATTGAGACTGAGGCTTGGGAAGATGCGGCGAAAGAGTACCAGGAGCTTTTGGCTGATATGTGTGAGCAGAAGCTGGCGCCCAATTTGCCTTATGTGAAGTCATTGTTTCTGGGGTGGTTTGAGCCATTGAGGGATGCCATTGCCGCCGAGCCCGCCACTTGGGCCGGTAAGAGGGTGCATATCAGACCCAGCCATGCTCCATTCTTCGAACTGTTGCCGCCCGATAAGATGGCTGTTATTACCATGCATAAGTTGATGGGGCTGTTGATGACCAACAGTGGTGGAGTTGGGTCTGTCAAGGTGGTTCAAGCTGCTTGTGCAATTGGTGAAGCTCTTGAGAATGAGGTTAAGATACACAGATTTTTGGAGAAGACAAAGAAGAAGAGCATTGCTATTAAGAAACCTGAAGCTGAATCTGATATTGTGACCAATGAACAAGAGAAACTTACCAACGAACAGGAGCAAGAGAAACTAACCAAAGAACAGGAGCTAGAAAAACTAACCAAAGAACAAAGACTGAGGAAAAAGGTTACAAAGTTGCTAAAAATGCAGAAGCTTCAGCGAGTAAAGGAAATTGTGAAGGAACAAGACGGCTTAAAGCCATGGGGTCAAGAAGCTCATGTTAAGGTCGGCTGTCGATTGATTCAGTTAATGATGGAAACAGCTTATATACAACCTGCACTTGATCAATTAGGAGATGCTCCACCTGATATTCGCCCGGCATTCGTGCATACCCTTAAGACTATAACTAAAGAGGCACAGAAGACCAACAGGAGATATGGTGTTATTGAATGCGACCCATTAGTTCGCAAAGGGATGGAGAAAACAGCAAGGCACATGGTCATTCCTTATATGCCAATGTTGGTGCCTCCACTTAACTGGACAGGGTATGATAAAGGAGCATATTTATTCTTACCATCCTATGTTATGCGAACTCATGGAGCGAAACAACAGCGCCAAGTTGTTAAATCAACTCCTAGGAAGCAATTAGAACCTGTTTATGAGGCCCTGGATACTCTTGGGAATACCAAATGGAGGATAAACAGAAGGGTACTTGGTGTAGTGGACAGGATATGGGCTAGTGGAGGCTGTCTTGCTGACTTGGTTGACCGTGAAGATGTTCCTTTGCCTGAAGAGCCAGACACAGAAGATGAGGCAGAAATGAGAAAATGGAAATGGAAAGTTAAAGCTGTGAAAAAGAAGAATAGTGAGAGGCACTCACAGCGTTGTGATACAGAACTTAAACTTTCTGTAGCTCGAAAGATGAAGGATGAGGAAGGCTTCTACTATCCGCACAATCTTGATTTCCGTGGTCGTGCCTACCCCATGCACCCATATCTAAACCATCTTGGTTCCGATATGTGCAGAGGCATCCTGGAGTTCGCAGAGGGACGTCGTCTTGGAAAATCTGGCTTACGCTGGTTGAAGATACATCTGGCAAATTTATATGCTGGTGGAGTTGACAAGCTCTCCTATGATGATCGAGTAGCATTTACGGAAAACCATGTAGACGAAATTTTTGATTGCGCAGACAGACCTCTTGAAGGAAGGCGTTGGTGGTTGGGTGCGGAAGATCCTTTCCAATGCTTGGCAGCATGCATTAATCTCACTGAAGCATTGAGAAGCCCCTCACCGGAGACTACCATTTCTCATATGCCTGTTCACCAGGATGGTTCATGTAATGGTTTACAACACTATGCTGCCCTCGGAAGGGACAAGCTTGGAGCAGCTGCTGTAAATCTTGTTGGGGGAGAGAAACCGGCTGATGTTTACTCAGGAATTGCTGCCAGGGTACTCAATATAATGCGCACAGATGCAGAGAAAGATCCAGCTAGTAGTCCAACTTCATTGCACGCTAGGCTTTTAATTAATGAGGTCGATCGTAAATTAGTGAAGCAGACAGTTATGACCTCTGTATATGGTGTTACTTATATTGGTGCACGCGATCAAATTAAGAAGAGATTAAAGGAGCGTGGTACCATAGCAGATGACGCGATGCTGTTTGCCGCATCTTGCTATGCTGCTAGAACCACCTTGACTGCCCTAGGAGAGATGTTTGAAGCTGCAAGAAGTATTATGAGTTGGCTTGGTGAATGTGCGAAGGTGATAGCTGCAGAGAATCATCCAGTTCGATGGACCACACCCCTTGGACTTCCTGTTGTACAACCATACCGCCAATTAGGAAGGCATCTTATTAAGACTTCCCTTCAGGTGCTGACATTACAGAGAGAAACCGACAAGGTCATGGTTAAACGGCAGAGGACAGCCTTTCCCCCCAATTTTGTCCACTCCCTGGATGGATCCCACATGATGATGACTGCTCTTGCCTGCAAGAAGGCAGGCTTAAATTTTGCAGGAGTACATGATTCATATTGGACGCATGCAAGTGACGTCGACGAAATGAATAGGATACTGAGAGAAAAGTTTGTTGAACTCTACGAGAGACCAATACTGGAGAATTTGTTGGAGAGCTTTCAAAAGTCTTTCCCCACCATTAATTTTCCTCCCTTACCGGATCGCGGAGACTTTGATCTCAGACAGGTTCTCGGATCTACTTATTTCTTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

1005

Amino Acids

113.54

Weight (kDa)

9.06

Isoelectric Point (pI)

45.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	146 - 491	9.9e-84	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	615 - 979	2.4e-158	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68990 AT1G68990
fragaria_vesca	FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970
malus_domestica	MD13G1055100.v1.1 MD16G1054000.v1.1
prunus_persica	Prupe.1G295700_v2.0.a1 Prupe.1G295700_v2.0.a1
pyrus_communis	pycom13g04880 pycom16g04700
rosa_chinensis	RchiOBHm_Chr4g0437111
rosa_laevigata	RLG00000006467
rosa_multiflora	Rmu_co8109606.1_g000001 Rmu_ssc0000006.1_g000008
rosa_roxburghii	Rroxscaffold_5G00378180
rosa_rugosa	Rorug04G0298100
rosa_samantha	Rh4AG352900 Rh4BG362400 Rh4CG376600 Rh4DG356400
rosa_wichuraiana	Rw4G030900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1843, 2841
Acc16I	TGCGCA	2 cut(s)	1975, 2255
Acc36I	ACCTGC	1 cut(s)	1132
Acc65I	GGTACC	1 cut(s)	2423
AccB1I	GGYRCC	3 cut(s)	523, 1312, 2423
AccB7I	CCANNNNNTGG	2 cut(s)	2035, 2740
AccI	GTMKAC	2 cut(s)	1956, 2841
AccII	CGCG	4 cut(s)	138, 2391, 2441, 2967
AciI	CCGC	9 cut(s)	473, 591, 600, 665, 1739, 2015, 2454, 2620, 2967
AclWI	GGATC	6 cut(s)	2015, 2267, 2742, 2755, 2970, 3004
AcsI	RAATTY	4 cut(s)	1888, 1962, 2794, 2905
AcuI	CTGAAG	6 cut(s)	336, 873, 989, 1599, 2079, 2639
AcyI	GRCGYC	3 cut(s)	524, 1840, 2838
AfaI	GTAC	7 cut(s)	419, 485, 1500, 2241, 2425, 2610, 2808
AflII	CTTAAG	1 cut(s)	1184
AjnI	CCWGG	7 cut(s)	177, 486, 1455, 1821, 2112, 2234, 2739
AjuI	GAANNNNNNNTTGG	2 cut(s)	194, 226
Alw21I	GWGCWC	1 cut(s)	2389
Alw26I	GTCTC	4 cut(s)	446, 2078, 2884, 2964
Alw44I	GTGCAC	1 cut(s)	2385
AlwI	GGATC	6 cut(s)	2015, 2267, 2742, 2755, 2970, 3004
AlwNI	CAGNNNCTG	3 cut(s)	520, 2180, 2662
Ama87I	CYCGRG	1 cut(s)	235
AoxI	GGCC	3 cut(s)	175, 609, 1452
ApaLI	GTGCAC	1 cut(s)	2385
ApoI	RAATTY	4 cut(s)	1888, 1962, 2794, 2905
ArsI	GACNNNNNNTTYG	2 cut(s)	1949, 1981
AseI	ATTAAT	3 cut(s)	2049, 2316, 2943
Asp700I	GAANNNNTTC	2 cut(s)	321, 1566
Asp718I	GGTACC	1 cut(s)	2423
AspA2I	CCTAGG	2 cut(s)	1424, 2491
AspLEI	GCGC	4 cut(s)	526, 1404, 1976, 2256
AspS9I	GGNCC	3 cut(s)	609, 1453, 2583
AsuC2I	CCSGG	1 cut(s)	1166
AsuHPI	GGTGA	6 cut(s)	358, 785, 2070, 2102, 2549, 2563
AsuII	TTCGAA	1 cut(s)	654
AvaI	CYCGRG	1 cut(s)	235
AvaII	GGWCC	1 cut(s)	2583
AvrII	CCTAGG	2 cut(s)	1424, 2491
BaeGI	GKGCMC	1 cut(s)	2389
BamHI	GGATCC	1 cut(s)	2747
BanI	GGYRCC	3 cut(s)	523, 1312, 2423
BanII	GRGCYC	1 cut(s)	600
BarI	GAAGNNNNNNTAC	2 cut(s)	2275, 2307
BauI	CACGAG	1 cut(s)	246
BbsI	GAAGAC	3 cut(s)	150, 824, 1217
Bbv12I	GWGCWC	1 cut(s)	2389
BceAI	ACGGC	3 cut(s)	436, 1054, 2717
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciT130I	CCWGG	7 cut(s)	179, 488, 1457, 1823, 2114, 2236, 2741
BciVI	GTATCC	2 cut(s)	1453, 2850
BclI	TGATCA	1 cut(s)	1132
BcnI	CCSGG	1 cut(s)	1166
BcoDI	GTCTC	4 cut(s)	446, 2078, 2884, 2964
BfaI	CTAG	9 cut(s)	14, 938, 1425, 1527, 2280, 2304, 2472, 2492, 3016
BfmI	CTRYAG	3 cut(s)	142, 1698, 2559
BfoI	RGCGCY	2 cut(s)	527, 1405
BfrI	CTTAAG	1 cut(s)	1184
BfuAI	ACCTGC	1 cut(s)	1132
BfuI	GTATCC	2 cut(s)	1453, 2850
BglI	GCCNNNNNGGC	1 cut(s)	608
BlnI	CCTAGG	2 cut(s)	1424, 2491
Bme1390I	CCNGG	8 cut(s)	179, 488, 1166, 1457, 1823, 2114, 2236, 2741
Bme18I	GGWCC	1 cut(s)	2583
BmeT110I	CYCGRG	1 cut(s)	235
BmgT120I	GGNCC	3 cut(s)	609, 1453, 2583
BmiI	GGNNCC	5 cut(s)	525, 1314, 1801, 2425, 2749
BmrFI	CCNGG	8 cut(s)	179, 488, 1166, 1457, 1823, 2114, 2236, 2741
BmrI	ACTGGG	1 cut(s)	216
BmsI	GCATC	9 cut(s)	200, 460, 571, 1135, 1827, 2251, 2433, 2465, 2644
BmuI	ACTGGG	1 cut(s)	216
BpiI	GAAGAC	3 cut(s)	150, 824, 1217
BplI	GAGNNNNNCTC	4 cut(s)	229, 261, 1649, 1681
BpmI	CTGGAG	4 cut(s)	131, 356, 1844, 2921
Bpu14I	TTCGAA	1 cut(s)	654
BpuEI	CTTGAG	2 cut(s)	78, 803
BpuMI	CCSGG	1 cut(s)	1166
BsaHI	GRCGYC	3 cut(s)	524, 1840, 2838
BsaI	GGTCTC	1 cut(s)	2884
BsaWI	WCCGGW	2 cut(s)	2080, 2959
BsaXI	ACNNNNNCTCC	6 cut(s)	1213, 1243, 1901, 1931, 2894, 2924
Bse118I	RCCGGY	2 cut(s)	611, 2206
Bse1I	ACTGG	4 cut(s)	222, 1331, 2573, 2904
Bse3DI	GCAATG	3 cut(s)	585, 834, 2294
BseBI	CCWGG	7 cut(s)	179, 488, 1457, 1823, 2114, 2236, 2741
BseGI	GGATG	9 cut(s)	304, 586, 1269, 1364, 1723, 1818, 2122, 2569, 2749
BseMI	GCAATG	3 cut(s)	585, 834, 2294
BseMII	CTCAG	6 cut(s)	419, 447, 956, 2235, 2852, 2995
BseNI	ACTGG	4 cut(s)	222, 1331, 2573, 2904
BseSI	GKGCMC	1 cut(s)	2389
BseYI	CCCAGC	1 cut(s)	635
BsgI	GTGCAG	2 cut(s)	1110, 1831
Bsh1236I	CGCG	4 cut(s)	138, 2391, 2441, 2967
Bsh1285I	CGRYCG	1 cut(s)	2329
BshFI	GGCC	3 cut(s)	177, 611, 1454
BshNI	GGYRCC	3 cut(s)	523, 1312, 2423
BsiEI	CGRYCG	1 cut(s)	2329
BsiHKAI	GWGCWC	1 cut(s)	2389
BsiHKCI	CYCGRG	1 cut(s)	235
BsiSI	CCGG	5 cut(s)	612, 1166, 2081, 2207, 2960
BslFI	GGGAC	3 cut(s)	181, 1851, 2174
BsmAI	GTCTC	4 cut(s)	446, 2078, 2884, 2964
BsmFI	GGGAC	3 cut(s)	181, 1851, 2174
BsmI	GAATGC	3 cut(s)	423, 1169, 1244
BsnI	GGCC	3 cut(s)	177, 611, 1454
Bso31I	GGTCTC	1 cut(s)	2884
BsoBI	CYCGRG	1 cut(s)	235
Bsp119I	TTCGAA	1 cut(s)	654
Bsp1286I	GDGCHC	2 cut(s)	600, 2389
Bsp1407I	TGTACA	1 cut(s)	2608
Bsp19I	CCATGG	1 cut(s)	1049
BspACI	CCGC	9 cut(s)	473, 591, 600, 665, 1739, 2015, 2454, 2620, 2967
BspANI	GGCC	3 cut(s)	177, 611, 1454
BspCNI	CTCAG	6 cut(s)	420, 448, 957, 2234, 2853, 2994
BspFNI	CGCG	4 cut(s)	138, 2391, 2441, 2967
BspLI	GGNNCC	5 cut(s)	525, 1314, 1801, 2425, 2749
BspMAI	CTGCAG	2 cut(s)	146, 2563
BspMI	ACCTGC	1 cut(s)	1132
BspPI	GGATC	6 cut(s)	2015, 2267, 2742, 2755, 2970, 3004
BspQI	GCTCTTC	2 cut(s)	824, 1575
BspT104I	TTCGAA	1 cut(s)	654
BspT107I	GGYRCC	3 cut(s)	523, 1312, 2423
BspTI	CTTAAG	1 cut(s)	1184
BspTNI	GGTCTC	1 cut(s)	2884
BsrDI	GCAATG	3 cut(s)	585, 834, 2294
BsrFI	RCCGGY	2 cut(s)	611, 2206
BsrGI	TGTACA	1 cut(s)	2608
BsrI	ACTGG	4 cut(s)	222, 1331, 2573, 2904
BssAI	RCCGGY	2 cut(s)	611, 2206
BssNI	GRCGYC	3 cut(s)	524, 1840, 2838
BssSI	CACGAG	1 cut(s)	246
BssT1I	CCWWGG	4 cut(s)	1049, 1424, 2491, 2593
Bst2BI	CACGAG	1 cut(s)	246
Bst2UI	CCWGG	7 cut(s)	179, 488, 1457, 1823, 2114, 2236, 2741
Bst4CI	ACNGT	4 cut(s)	660, 725, 1559, 2350
Bst6I	CTCTTC	4 cut(s)	312, 824, 1575, 2399
BstACI	GRCGYC	3 cut(s)	524, 1840, 2838
BstAFI	CTTAAG	1 cut(s)	1184
BstAUI	TGTACA	1 cut(s)	2608
BstBI	TTCGAA	1 cut(s)	654
BstDEI	CTNAG	6 cut(s)	428, 456, 965, 2221, 2861, 2981
BstDSI	CCRYGG	3 cut(s)	294, 1049, 1756
BstENI	CCTNNNNNAGG	1 cut(s)	1989
BstF5I	GGATG	9 cut(s)	304, 586, 1269, 1364, 1723, 1818, 2122, 2569, 2749
BstFNI	CGCG	4 cut(s)	138, 2391, 2441, 2967
BstH2I	RGCGCY	2 cut(s)	527, 1405
BstHHI	GCGC	4 cut(s)	526, 1404, 1976, 2256
BstMAI	GTCTC	4 cut(s)	446, 2078, 2884, 2964
BstMCI	CGRYCG	1 cut(s)	2329
BstNI	CCWGG	7 cut(s)	179, 488, 1457, 1823, 2114, 2236, 2741
BstNSI	RCATGY	2 cut(s)	2046, 2831
BstSCI	CCNGG	8 cut(s)	177, 486, 1164, 1455, 1821, 2112, 2234, 2739
BstSFI	CTRYAG	3 cut(s)	142, 1698, 2559
BstSLI	GKGCMC	1 cut(s)	2389
BstUI	CGCG	4 cut(s)	138, 2391, 2441, 2967
BstV2I	GAAGAC	3 cut(s)	150, 824, 1217
BstX2I	RGATCY	4 cut(s)	2020, 2272, 2747, 2996
BstXI	CCANNNNNNTGG	4 cut(s)	185, 391, 1326, 2580
BstYI	RGATCY	4 cut(s)	2020, 2272, 2747, 2996
BsuI	GTATCC	2 cut(s)	1453, 2850
BsuRI	GGCC	3 cut(s)	177, 611, 1454
BtgI	CCRYGG	3 cut(s)	294, 1049, 1756
BtgZI	GCGATG	1 cut(s)	2456
BtsCI	GGATG	9 cut(s)	304, 586, 1269, 1364, 1723, 1818, 2122, 2569, 2749
BtsIMutI	CAGTG	2 cut(s)	730, 2055
BveI	ACCTGC	1 cut(s)	1132
CaiI	CAGNNNCTG	3 cut(s)	520, 2180, 2662
CfoI	GCGC	4 cut(s)	526, 1404, 1976, 2256
Cfr10I	RCCGGY	2 cut(s)	611, 2206
Cfr13I	GGNCC	3 cut(s)	609, 1453, 2583
CseI	GACGC	3 cut(s)	127, 2447, 2833
Csp6I	GTAC	7 cut(s)	418, 484, 1499, 2240, 2424, 2609, 2807
CspCI	CAANNNNNGTGG	4 cut(s)	709, 744, 2575, 2610
CviQI	GTAC	7 cut(s)	418, 484, 1499, 2240, 2424, 2609, 2807
DdeI	CTNAG	6 cut(s)	428, 456, 965, 2221, 2861, 2981
DinI	GGCGCC	1 cut(s)	525
Eam1104I	CTCTTC	4 cut(s)	312, 824, 1575, 2399
EarI	CTCTTC	4 cut(s)	312, 824, 1575, 2399
Eco130I	CCWWGG	4 cut(s)	1049, 1424, 2491, 2593
Eco24I	GRGCYC	1 cut(s)	600
Eco31I	GGTCTC	1 cut(s)	2884
Eco47I	GGWCC	1 cut(s)	2583
Eco57I	CTGAAG	6 cut(s)	336, 873, 989, 1599, 2079, 2639
Eco88I	CYCGRG	1 cut(s)	235
EcoNI	CCTNNNNNAGG	1 cut(s)	1989
EcoO109I	RGGNCCY	1 cut(s)	1453
EcoRII	CCWGG	7 cut(s)	177, 486, 1455, 1821, 2112, 2234, 2739
EcoT14I	CCWWGG	4 cut(s)	1049, 1424, 2491, 2593
EcoT22I	ATGCAT	2 cut(s)	696, 2048
EcoT38I	GRGCYC	1 cut(s)	600
EgeI	GGCGCC	1 cut(s)	525
EheI	GGCGCC	1 cut(s)	525
ErhI	CCWWGG	4 cut(s)	1049, 1424, 2491, 2593
FalI	AAGNNNNNCTT	4 cut(s)	876, 908, 2774, 2806
FaqI	GGGAC	3 cut(s)	181, 1851, 2174
FauI	CCCGC	1 cut(s)	607
FauNDI	CATATG	1 cut(s)	2100
FbaI	TGATCA	1 cut(s)	1132
FblI	GTMKAC	2 cut(s)	1956, 2841
FokI	GGATG	9 cut(s)	311, 593, 1276, 1351, 1730, 1805, 2129, 2556, 2756
FriOI	GRGCYC	1 cut(s)	600
FspAI	RTGCGCAY	1 cut(s)	2255
FspBI	CTAG	9 cut(s)	14, 938, 1425, 1527, 2280, 2304, 2472, 2492, 3016
FspI	TGCGCA	2 cut(s)	1975, 2255
GlaI	GCGC	4 cut(s)	525, 1403, 1975, 2255
GsaI	CCCAGC	1 cut(s)	639
GsuI	CTGGAG	4 cut(s)	131, 356, 1844, 2921
HaeII	RGCGCY	2 cut(s)	527, 1405
HaeIII	GGCC	3 cut(s)	177, 611, 1454
HapII	CCGG	5 cut(s)	612, 1166, 2081, 2207, 2960
HgaI	GACGC	3 cut(s)	127, 2447, 2833
HhaI	GCGC	4 cut(s)	526, 1404, 1976, 2256
Hin1I	GRCGYC	3 cut(s)	524, 1840, 2838
Hin6I	GCGC	4 cut(s)	524, 1402, 1974, 2254
HinP1I	GCGC	4 cut(s)	524, 1402, 1974, 2254
HincII	GTYRAC	3 cut(s)	1555, 1909, 2842
HindII	GTYRAC	3 cut(s)	1555, 1909, 2842
HindIII	AAGCTT	3 cut(s)	50, 1001, 2165
HinfI	GANTC	8 cut(s)	98, 305, 322, 365, 860, 1090, 2566, 2813
HpaII	CCGG	5 cut(s)	612, 1166, 2081, 2207, 2960
HphI	GGTGA	6 cut(s)	358, 785, 2070, 2102, 2549, 2563
Hpy188I	TCNGA	9 cut(s)	84, 316, 429, 632, 865, 1804, 2156, 2984, 2996
Hpy188III	TCNNGA	8 cut(s)	95, 110, 326, 782, 1058, 1748, 1988, 2223
Hpy99I	CGWCG	3 cut(s)	1845, 2843, 2846
HpyCH4III	ACNGT	4 cut(s)	660, 725, 1559, 2350
HpyCH4IV	ACGT	2 cut(s)	1840, 2838
HpyF3I	CTNAG	6 cut(s)	428, 456, 965, 2221, 2861, 2981
HpySE526I	ACGT	2 cut(s)	1840, 2838
Hsp92I	GRCGYC	3 cut(s)	524, 1840, 2838
HspAI	GCGC	4 cut(s)	524, 1402, 1974, 2254
KasI	GGCGCC	1 cut(s)	523
KpnI	GGTACC	1 cut(s)	2427
Ksp22I	TGATCA	1 cut(s)	1132
LguI	GCTCTTC	2 cut(s)	824, 1575
LweI	GCATC	9 cut(s)	200, 460, 571, 1135, 1827, 2251, 2433, 2465, 2644
MaeI	CTAG	9 cut(s)	14, 938, 1425, 1527, 2280, 2304, 2472, 2492, 3016
MaeII	ACGT	2 cut(s)	1840, 2838
MaeIII	GTNAC	5 cut(s)	239, 871, 976, 2371, 2834
MfeI	CAATTG	1 cut(s)	768
MflI	RGATCY	4 cut(s)	2020, 2272, 2747, 2996
MhlI	GDGCHC	2 cut(s)	600, 2389
Mly113I	GGCGCC	1 cut(s)	524
MlyI	GAGTC	1 cut(s)	359
MmeI	TCCRAC	2 cut(s)	2312, 2892
Mph1103I	ATGCAT	2 cut(s)	696, 2048
MroXI	GAANNNNTTC	2 cut(s)	321, 1566
MslI	CAYNNNNRTG	3 cut(s)	2115, 2759, 2832
MspA1I	CMGCKG	1 cut(s)	2177
MspCI	CTTAAG	1 cut(s)	1184
MspI	CCGG	5 cut(s)	612, 1166, 2081, 2207, 2960
MspR9I	CCNGG	8 cut(s)	179, 488, 1166, 1457, 1823, 2114, 2236, 2741
MunI	CAATTG	1 cut(s)	768
Mva1269I	GAATGC	3 cut(s)	423, 1169, 1244
MvaI	CCWGG	7 cut(s)	179, 488, 1457, 1823, 2114, 2236, 2741
MvnI	CGCG	4 cut(s)	138, 2391, 2441, 2967
NarI	GGCGCC	1 cut(s)	524
NciI	CCSGG	1 cut(s)	1166
NcoI	CCATGG	1 cut(s)	1049
NdeI	CATATG	1 cut(s)	2100
NlaIV	GGNNCC	5 cut(s)	525, 1314, 1801, 2425, 2749
NmeAIII	GCCGAG	2 cut(s)	304, 619
NmuCI	GTSAC	2 cut(s)	871, 2834
NsbI	TGCGCA	2 cut(s)	1975, 2255
NsiI	ATGCAT	2 cut(s)	696, 2048
NspI	RCATGY	2 cut(s)	2046, 2831
NspV	TTCGAA	1 cut(s)	654
PacI	TTAATTAA	1 cut(s)	2316
PaeI	GCATGC	2 cut(s)	2046, 2831
PciSI	GCTCTTC	2 cut(s)	824, 1575
PctI	GAATGC	3 cut(s)	423, 1169, 1244
PdmI	GAANNNNTTC	2 cut(s)	321, 1566
PfeI	GAWTC	7 cut(s)	98, 305, 322, 860, 1090, 2566, 2813
PflFI	GACNNNGTC	2 cut(s)	1842, 2687
PflMI	CCANNNNNTGG	2 cut(s)	2035, 2740
PfoI	TCCNGGA	1 cut(s)	1821
Ple19I	CGATCG	1 cut(s)	2329
PleI	GAGTC	1 cut(s)	359
PluTI	GGCGCC	1 cut(s)	527
PpsI	GAGTC	1 cut(s)	359
PshBI	ATTAAT	3 cut(s)	2049, 2316, 2943
Psp6I	CCWGG	7 cut(s)	177, 486, 1455, 1821, 2112, 2234, 2739
PspFI	CCCAGC	1 cut(s)	635
PspGI	CCWGG	7 cut(s)	177, 486, 1455, 1821, 2112, 2234, 2739
PspN4I	GGNNCC	5 cut(s)	525, 1314, 1801, 2425, 2749
PspPI	GGNCC	3 cut(s)	609, 1453, 2583
PsrI	GAACNNNNNNTAC	2 cut(s)	224, 256
PstI	CTGCAG	2 cut(s)	146, 2563
PstNI	CAGNNNCTG	3 cut(s)	520, 2180, 2662
PsuI	RGATCY	4 cut(s)	2020, 2272, 2747, 2996
PsyI	GACNNNGTC	2 cut(s)	1842, 2687
PvuI	CGATCG	1 cut(s)	2329
PvuII	CAGCTG	1 cut(s)	2177
RsaI	GTAC	7 cut(s)	419, 485, 1500, 2241, 2425, 2610, 2808
RsaNI	GTAC	7 cut(s)	418, 484, 1499, 2240, 2424, 2609, 2807
RseI	CAYNNNNRTG	3 cut(s)	2115, 2759, 2832
SalI	GTCGAC	1 cut(s)	2840
SapI	GCTCTTC	2 cut(s)	824, 1575
Sau96I	GGNCC	3 cut(s)	609, 1453, 2583
SchI	GAGTC	1 cut(s)	359
ScrFI	CCNGG	8 cut(s)	179, 488, 1166, 1457, 1823, 2114, 2236, 2741
SduI	GDGCHC	2 cut(s)	600, 2389
SfaNI	GCATC	9 cut(s)	200, 460, 571, 1135, 1827, 2251, 2433, 2465, 2644
SfcI	CTRYAG	3 cut(s)	142, 1698, 2559
SfoI	GGCGCC	1 cut(s)	525
SfuI	TTCGAA	1 cut(s)	654
SgrDI	CGTCGACG	1 cut(s)	2840
SinI	GGWCC	1 cut(s)	2583
SmiMI	CAYNNNNRTG	3 cut(s)	2115, 2759, 2832
SmlI	CTYRAG	3 cut(s)	93, 782, 1184
SmoI	CTYRAG	3 cut(s)	93, 782, 1184
SphI	GCATGC	2 cut(s)	2046, 2831
SsiI	CCGC	9 cut(s)	473, 591, 600, 665, 1739, 2015, 2454, 2620, 2967
SspDI	GGCGCC	1 cut(s)	523
SspMI	CTAG	9 cut(s)	14, 938, 1425, 1527, 2280, 2304, 2472, 2492, 3016
StyD4I	CCNGG	8 cut(s)	177, 486, 1164, 1455, 1821, 2112, 2234, 2739
StyI	CCWWGG	4 cut(s)	1049, 1424, 2491, 2593
TaaI	ACNGT	4 cut(s)	660, 725, 1559, 2350
TaiI	ACGT	2 cut(s)	1843, 2841
TaqI	TCGA	8 cut(s)	101, 654, 1084, 1705, 1930, 2325, 2578, 2841
TatI	WGTACW	2 cut(s)	2608, 2806
TauI	GCSGC	4 cut(s)	476, 593, 667, 2456
TfiI	GAWTC	7 cut(s)	98, 305, 322, 860, 1090, 2566, 2813
TscAI	CASTG	2 cut(s)	730, 2062
TseFI	GTSAC	2 cut(s)	871, 2834
Tsp45I	GTSAC	2 cut(s)	871, 2834
TspDTI	ATGAA	6 cut(s)	894, 1727, 2112, 2283, 2805, 2864
TspGWI	ACGGA	4 cut(s)	222, 311, 1745, 1958
TspRI	CASTG	2 cut(s)	730, 2062
Tth111I	GACNNNGTC	2 cut(s)	1842, 2687
Van91I	CCANNNNNTGG	2 cut(s)	2035, 2740
Vha464I	CTTAAG	1 cut(s)	1184
VneI	GTGCAC	1 cut(s)	2385
VpaK11BI	GGWCC	1 cut(s)	2583
VspI	ATTAAT	3 cut(s)	2049, 2316, 2943
XagI	CCTNNNNNAGG	1 cut(s)	1989
XapI	RAATTY	4 cut(s)	1888, 1962, 2794, 2905
XceI	RCATGY	2 cut(s)	2046, 2831
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2741
XmaJI	CCTAGG	2 cut(s)	1424, 2491
XmiI	GTMKAC	2 cut(s)	1956, 2841
XmnI	GAANNNNTTC	2 cut(s)	321, 1566
XspI	CTAG	9 cut(s)	14, 938, 1425, 1527, 2280, 2304, 2472, 2492, 3016
ZraI	GACGTC	2 cut(s)	1841, 2839
Zsp2I	ATGCAT	2 cut(s)	696, 2048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.