Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0007623

Physical Location & Seq

Genomic Coordinates Forward (+)

7475131 .. 7482341

Gene Structure 7211 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006467

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGTGGAGAACCCTAGCCAAACAAGCTGCTTCTTCTCCAACAAAGACCAAGCTTTTCTCTCACTCTCCATTCAGCTCTGCTTTGAAACTTGCTCAAGAATCGAGCTTTCTGGAGAAAAGCAGGCATTTTGAGGCTCGCGTCTGCAGAAATTATGGGTCTTCGTTAATGGGTTTAGGCCAGGTTGGTGGTGCATCGTCCCAGAGAGACGAATTGGGCAGACCCAGTTTGCTGAATCCGAGTTACCCTTGTGGGTTTGGGAGATGGTATGCTAGTTCTGCCGAGGCTATTGCTTCCACGGATGAGGAATCTGGGTCTGAAGAGATTCAGGAAATGCTGGAGGATTTGGTTAGGGAGAACAAGGTGGAGTCGCATTTGAGGCAGCCCAAGAGGTTGGTTAATGGAATGGGAGCAGGAAAGTATGGCATTCTGAGAAAGAGGCAGTTAAAAATTGAGACTGAGGCTTGGGAAGATGCAGCCAACGAGTACCAGGAGCTTTTGGCTGATATGTGTGAGCAGAAGCTGGCGCCGAATTTGCCTTATGTGAAGTCATTGTTTCTGGGGTGGTTTGAGCCATTGAGGGATGCCATTGCTGCCGAGCCCGCGACTTGGGCCGGTAAGAGGGTGCATATCAGACCCAGCCATGCTCCATTTTTCGAACTGTTGCCGCCGGATAAGATGGCTGTTATTACAATGCATAAGTTGATGGGGCTGTTGATGACCAACAGTGGTGGAGTTGGGTCTGTCAAGGTGGTTCAAGCTGCTTGTGCAATTGGTGAAGCTCTTGAGAATGAGGTTAAGATACACAGATTTTTGGAGAAGACAAAGAAGAAGAGCACTGTGATTAAAAAACCTGAAGCTGAATCTGATTTTGTGACCTATGAACAAGAGAAACTTACCAACGAACAGGAGCAAGAGAAACTAACCAAAGAACAGGAGCTAGAAAAACTAACCAAAGAACAAAGACTGAGGAAAAAGGTTACAAAGTTGCTAAAAATGCAGAAGCTTCAGCGAGTAAAGGAAATTGTGAAGGAACAAGACGGCTTAAAGCCATGGGGCCAAGAAGCTCATGTTAAGGTCGGCTGTCGATTGATTCAGTTAATGATGGAAACAGCTTATATACAACCTGCACTTGATCAATTAGGAGATTCTCCGCCTGATATTCGCCCGGCATTCGTGCATACCCTTAAGACTATAACTAAAGAGGCACAGCACATGGTCATTCCTTATATGCCAATGTTGGTGCCTCCACTTAACTGGACAGGGTATGATAAGGGAGCATATTTATTTTTACCATCCTATGTTATGCGAACTCATGGAGCGAAACAACAGCGCCAAGTTGTTAAATCGACTCCTAGGAAGCAATTAGAACCTGTTTATGAGGCCCTGGATACTCTTGGAAATACCAAATGGAGGATAAACAGAAGGGTACTTGGTGTAGTGGACAGGATATGGGCTAGTGGAGGCTGTCTTGCTGACTTGGTTGACCGTGAAGATGTTCCTTTGCCTGAAGAACCAGACACAGAAGATGAGGCAGAAATGAGAAAATGGAAATGGAAAGTTAAAGCTGTGAAAAAGAAGAATAGTGAGAGGCACTCACAGCGCTGTGATACAGAACTTAAACTTTCTGTAGCTCGAAAGATGAAGGACGAGGAAGGCTTCTACTATCCGCACAATCTTGATTTCCGTGGTCGTGCCTACCCCATGCACCCATATCTAAATCATCTTGGTTCCGATATGTGCAGAGGCATCCTGGAGTTCGCAGAGGGACGTCGTCTAGGAAAATCTGGCTTACGCTGGTTGAAGATACATCTGGCAAATTTATATGCTGGTGGAGTTGACAAGCTCTCCTATGATGATCGAGTAACATTTACGGAAAACCATGTAGACGAAATTTTTGATTGCGCAGACAGACCTCTTGAAGGAAGGCGTTGGTGGTTGGGTGCGGAAGATCCTTTCCAATGCTTGGCAGCATGCATTAATCTCACTGAAGCATTGAGAAGCCCCTCACCGGAGACTACCATTTCTCATATGCCTGTTCACCAGGATGGTTCATGTAATGGTTTACAACACTATGCTGCCCTCGGAAGGGACAAGGTCGATCGTAAATTGGTGAAGCAGACAGTAATGACCTCTGTATATGGTGTTACTTATATTGGTGCACGTGATCAAATTAAGAAGAGATTAAAGGAGCGTGGTACCATAGCAGATGACACGATGCTGTTTGCCGCATCTTGCTATGCTGCTAGAACCACCTTGACTGCCCTAGGAGAGATGTTTGAAGCTGCAAGAAGTATTATGAGTTGGCTTGGTGAATGTGCGAAGGTGATAGCTGCAGAGAATCATCCAGTTCGATGGATCACACCCCTTGGACTTCCTGTTGTACAACCATACCGCCAATTAGGAAGGCATCTTATTAAGACTTCCCTTCAGGTGCTGACATTACAGAGAGAAACCGACAAGGTCATGGTTAAACGGCAGAGGACAGCCTTTCCCCCCAATTTTGTCCACTCCCTGGATGGATCCCACATGATGATGACTGCTCTTGCCTGCAAGAAGGCAGGCTTAAATTTTGCAGGAGTACATGATTCATATTGGACGCATGCAAGTGACGTCGACGAAATGAATAGGATACTGAGAGAAAAGTTTGTTGAACTCTACGAGAGACCAATACTGGAGAATTTGTTGGAGAGCTTTCAAAAGTCTTTCCCCACCATTACTTTTCCTCCCTTACCGGATCGCGGAGACTTTGATCTCAGACAGGTTCTCGGATCTACTTATTTCTTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

930

Amino Acids

105.7

Weight (kDa)

8.86

Isoelectric Point (pI)

47.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	146 - 467	8e-71	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	591 - 699	8.2e-51	DNA-dependent RNA polymerase
RNA_pol	PF00940	697 - 903	1.4e-86	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68990 AT1G68990
fragaria_vesca	FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970 FvH4_4g28970
malus_domestica	MD13G1055100.v1.1 MD16G1054000.v1.1
prunus_persica	Prupe.1G295700_v2.0.a1 Prupe.1G295700_v2.0.a1
pyrus_communis	pycom13g04880 pycom16g04700
rosa_chinensis	RchiOBHm_Chr4g0437111
rosa_laevigata	RLG00000006467
rosa_multiflora	Rmu_co8109606.1_g000001 Rmu_ssc0000006.1_g000008
rosa_roxburghii	Rroxscaffold_5G00378180
rosa_rugosa	Rorug04G0298100
rosa_samantha	Rh4AG352900 Rh4BG362400 Rh4CG376600 Rh4DG356400
rosa_wichuraiana	Rw4G030900

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1771, 2613
Acc16I	TGCGCA	1 cut(s)	1903
Acc36I	ACCTGC	1 cut(s)	1132
Acc65I	GGTACC	1 cut(s)	2195
AccB1I	GGYRCC	3 cut(s)	523, 1240, 2195
AccB7I	CCANNNNNTGG	2 cut(s)	1963, 2512
AccI	GTMKAC	2 cut(s)	1884, 2613
AccII	CGCG	3 cut(s)	138, 602, 2739
AciI	CCGC	8 cut(s)	600, 665, 1151, 1667, 1943, 2226, 2392, 2739
AclWI	GGATC	6 cut(s)	1943, 2363, 2514, 2527, 2742, 2776
AcsI	RAATTY	5 cut(s)	529, 1816, 1890, 2566, 2677
AcuI	CTGAAG	6 cut(s)	336, 873, 989, 1527, 2007, 2411
AcvI	CACGTG	1 cut(s)	2162
AcyI	GRCGYC	3 cut(s)	524, 1768, 2610
AfaI	GTAC	5 cut(s)	485, 1428, 2197, 2382, 2580
AfeI	AGCGCT	1 cut(s)	1601
AflII	CTTAAG	1 cut(s)	1184
AgsI	TTSAA	8 cut(s)	85, 755, 1801, 1919, 2279, 2651, 2696, 2785
AjnI	CCWGG	6 cut(s)	177, 486, 1383, 1749, 2040, 2511
Alw21I	GWGCWC	2 cut(s)	836, 2161
Alw26I	GTCTC	5 cut(s)	198, 446, 2006, 2656, 2736
Alw44I	GTGCAC	1 cut(s)	2157
AlwI	GGATC	6 cut(s)	1943, 2363, 2514, 2527, 2742, 2776
AlwNI	CAGNNNCTG	2 cut(s)	520, 2434
Aor51HI	AGCGCT	1 cut(s)	1601
AoxI	GGCC	4 cut(s)	175, 609, 1054, 1380
ApaLI	GTGCAC	1 cut(s)	2157
ApoI	RAATTY	5 cut(s)	529, 1816, 1890, 2566, 2677
ArsI	GACNNNNNNTTYG	2 cut(s)	1877, 1909
AseI	ATTAAT	1 cut(s)	1977
Asp700I	GAANNNNTTC	2 cut(s)	321, 1494
Asp718I	GGTACC	1 cut(s)	2195
AspA2I	CCTAGG	2 cut(s)	1352, 2263
AspLEI	GCGC	4 cut(s)	526, 1332, 1602, 1904
AspS9I	GGNCC	3 cut(s)	609, 1054, 1381
AsuC2I	CCSGG	1 cut(s)	1166
AsuHPI	GGTGA	6 cut(s)	785, 1998, 2030, 2122, 2321, 2335
AsuII	TTCGAA	1 cut(s)	654
AvrII	CCTAGG	2 cut(s)	1352, 2263
BaeGI	GKGCMC	1 cut(s)	2161
BamHI	GGATCC	1 cut(s)	2519
BanI	GGYRCC	3 cut(s)	523, 1240, 2195
BanII	GRGCYC	1 cut(s)	600
BbrPI	CACGTG	1 cut(s)	2162
BbsI	GAAGAC	2 cut(s)	150, 824
Bbv12I	GWGCWC	2 cut(s)	836, 2161
BccI	CCATC	8 cut(s)	255, 670, 697, 1096, 1300, 2039, 2346, 2510
BceAI	ACGGC	2 cut(s)	1054, 2489
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciT130I	CCWGG	6 cut(s)	179, 488, 1385, 1751, 2042, 2513
BciVI	GTATCC	2 cut(s)	1381, 2622
BclI	TGATCA	2 cut(s)	1132, 2164
BcnI	CCSGG	1 cut(s)	1166
BcoDI	GTCTC	5 cut(s)	198, 446, 2006, 2656, 2736
BfaI	CTAG	9 cut(s)	14, 270, 938, 1353, 1455, 1775, 2244, 2264, 2788
BfmI	CTRYAG	3 cut(s)	142, 1626, 2331
BfoI	RGCGCY	3 cut(s)	527, 1333, 1603
BfrI	CTTAAG	1 cut(s)	1184
BfuAI	ACCTGC	1 cut(s)	1132
BfuI	GTATCC	2 cut(s)	1381, 2622
BlnI	CCTAGG	2 cut(s)	1352, 2263
Bme1390I	CCNGG	7 cut(s)	179, 488, 1166, 1385, 1751, 2042, 2513
BmgT120I	GGNCC	3 cut(s)	609, 1054, 1381
BmiI	GGNNCC	6 cut(s)	525, 1055, 1242, 1729, 2197, 2521
BmrFI	CCNGG	7 cut(s)	179, 488, 1166, 1385, 1751, 2042, 2513
BmrI	ACTGGG	1 cut(s)	216
BmsI	GCATC	7 cut(s)	200, 460, 571, 1755, 2205, 2237, 2416
BmuI	ACTGGG	1 cut(s)	216
BpiI	GAAGAC	2 cut(s)	150, 824
BplI	GAGNNNNNCTC	2 cut(s)	1577, 1609
BpmI	CTGGAG	4 cut(s)	131, 356, 1772, 2693
Bpu14I	TTCGAA	1 cut(s)	654
BpuEI	CTTGAG	2 cut(s)	78, 803
BpuMI	CCSGG	1 cut(s)	1166
BsaAI	YACGTR	1 cut(s)	2162
BsaHI	GRCGYC	3 cut(s)	524, 1768, 2610
BsaI	GGTCTC	1 cut(s)	2656
BsaWI	WCCGGW	2 cut(s)	2008, 2731
BsaXI	ACNNNNNCTCC	6 cut(s)	1829, 1859, 2666, 2696, 2707, 2737
Bse118I	RCCGGY	1 cut(s)	611
Bse1I	ACTGG	4 cut(s)	222, 1259, 2345, 2676
Bse3DI	GCAATG	1 cut(s)	585
BseBI	CCWGG	6 cut(s)	179, 488, 1385, 1751, 2042, 2513
BseGI	GGATG	7 cut(s)	304, 586, 1292, 1746, 2050, 2341, 2521
BseMI	GCAATG	1 cut(s)	585
BseMII	CTCAG	5 cut(s)	419, 447, 956, 2624, 2767
BseNI	ACTGG	4 cut(s)	222, 1259, 2345, 2676
BseSI	GKGCMC	1 cut(s)	2161
BseYI	CCCAGC	1 cut(s)	635
BsgI	GTGCAG	2 cut(s)	1110, 1759
Bsh1236I	CGCG	3 cut(s)	138, 602, 2739
Bsh1285I	CGRYCG	1 cut(s)	2101
BshFI	GGCC	4 cut(s)	177, 611, 1056, 1382
BshNI	GGYRCC	3 cut(s)	523, 1240, 2195
BsiEI	CGRYCG	1 cut(s)	2101
BsiHKAI	GWGCWC	2 cut(s)	836, 2161
BsiSI	CCGG	5 cut(s)	612, 668, 1166, 2009, 2732
BslFI	GGGAC	3 cut(s)	181, 1779, 2102
BsmAI	GTCTC	5 cut(s)	198, 446, 2006, 2656, 2736
BsmBI	CGTCTC	1 cut(s)	198
BsmFI	GGGAC	3 cut(s)	181, 1779, 2102
BsmI	GAATGC	2 cut(s)	423, 1169
BsnI	GGCC	4 cut(s)	177, 611, 1056, 1382
Bso31I	GGTCTC	1 cut(s)	2656
Bsp119I	TTCGAA	1 cut(s)	654
Bsp1286I	GDGCHC	3 cut(s)	600, 836, 2161
Bsp1407I	TGTACA	1 cut(s)	2380
Bsp19I	CCATGG	1 cut(s)	1049
BspACI	CCGC	8 cut(s)	600, 665, 1151, 1667, 1943, 2226, 2392, 2739
BspANI	GGCC	4 cut(s)	177, 611, 1056, 1382
BspCNI	CTCAG	5 cut(s)	420, 448, 957, 2625, 2766
BspFNI	CGCG	3 cut(s)	138, 602, 2739
BspLI	GGNNCC	6 cut(s)	525, 1055, 1242, 1729, 2197, 2521
BspMAI	CTGCAG	2 cut(s)	146, 2335
BspMI	ACCTGC	1 cut(s)	1132
BspPI	GGATC	6 cut(s)	1943, 2363, 2514, 2527, 2742, 2776
BspQI	GCTCTTC	1 cut(s)	824
BspT104I	TTCGAA	1 cut(s)	654
BspT107I	GGYRCC	3 cut(s)	523, 1240, 2195
BspTI	CTTAAG	1 cut(s)	1184
BspTNI	GGTCTC	1 cut(s)	2656
BsrDI	GCAATG	1 cut(s)	585
BsrFI	RCCGGY	1 cut(s)	611
BsrGI	TGTACA	1 cut(s)	2380
BsrI	ACTGG	4 cut(s)	222, 1259, 2345, 2676
BssAI	RCCGGY	1 cut(s)	611
BssNI	GRCGYC	3 cut(s)	524, 1768, 2610
BssT1I	CCWWGG	4 cut(s)	1049, 1352, 2263, 2365
Bst2UI	CCWGG	6 cut(s)	179, 488, 1385, 1751, 2042, 2513
Bst4CI	ACNGT	5 cut(s)	660, 725, 838, 1487, 2122
Bst6I	CTCTTC	3 cut(s)	312, 824, 2171
BstACI	GRCGYC	3 cut(s)	524, 1768, 2610
BstAFI	CTTAAG	1 cut(s)	1184
BstAUI	TGTACA	1 cut(s)	2380
BstBAI	YACGTR	1 cut(s)	2162
BstBI	TTCGAA	1 cut(s)	654
BstC8I	GCNNGC	8 cut(s)	122, 136, 522, 600, 1972, 2548, 2560, 2601
BstDEI	CTNAG	5 cut(s)	428, 456, 965, 2633, 2753
BstDSI	CCRYGG	3 cut(s)	294, 1049, 1684
BstENI	CCTNNNNNAGG	1 cut(s)	1917
BstF5I	GGATG	7 cut(s)	304, 586, 1292, 1746, 2050, 2341, 2521
BstFNI	CGCG	3 cut(s)	138, 602, 2739
BstH2I	RGCGCY	3 cut(s)	527, 1333, 1603
BstHHI	GCGC	4 cut(s)	526, 1332, 1602, 1904
BstMAI	GTCTC	5 cut(s)	198, 446, 2006, 2656, 2736
BstMCI	CGRYCG	1 cut(s)	2101
BstNI	CCWGG	6 cut(s)	179, 488, 1385, 1751, 2042, 2513
BstNSI	RCATGY	2 cut(s)	1974, 2603
BstSCI	CCNGG	7 cut(s)	177, 486, 1164, 1383, 1749, 2040, 2511
BstSFI	CTRYAG	3 cut(s)	142, 1626, 2331
BstSLI	GKGCMC	1 cut(s)	2161
BstUI	CGCG	3 cut(s)	138, 602, 2739
BstV2I	GAAGAC	2 cut(s)	150, 824
BstX2I	RGATCY	3 cut(s)	1948, 2519, 2768
BstXI	CCANNNNNNTGG	4 cut(s)	185, 391, 1254, 2352
BstYI	RGATCY	3 cut(s)	1948, 2519, 2768
BsuI	GTATCC	2 cut(s)	1381, 2622
BsuRI	GGCC	4 cut(s)	177, 611, 1056, 1382
BtgI	CCRYGG	3 cut(s)	294, 1049, 1684
BtsCI	GGATG	7 cut(s)	304, 586, 1292, 1746, 2050, 2341, 2521
BtsIMutI	CAGTG	3 cut(s)	730, 834, 1983
BveI	ACCTGC	1 cut(s)	1132
Cac8I	GCNNGC	8 cut(s)	122, 136, 522, 600, 1972, 2548, 2560, 2601
CaiI	CAGNNNCTG	2 cut(s)	520, 2434
CfoI	GCGC	4 cut(s)	526, 1332, 1602, 1904
Cfr10I	RCCGGY	1 cut(s)	611
Cfr13I	GGNCC	3 cut(s)	609, 1054, 1381
CseI	GACGC	2 cut(s)	127, 2605
Csp6I	GTAC	5 cut(s)	484, 1427, 2196, 2381, 2579
CspCI	CAANNNNNGTGG	2 cut(s)	709, 744
CviQI	GTAC	5 cut(s)	484, 1427, 2196, 2381, 2579
DdeI	CTNAG	5 cut(s)	428, 456, 965, 2633, 2753
DinI	GGCGCC	1 cut(s)	525
Eam1104I	CTCTTC	3 cut(s)	312, 824, 2171
EarI	CTCTTC	3 cut(s)	312, 824, 2171
EciI	GGCGGA	1 cut(s)	1140
Eco130I	CCWWGG	4 cut(s)	1049, 1352, 2263, 2365
Eco24I	GRGCYC	1 cut(s)	600
Eco31I	GGTCTC	1 cut(s)	2656
Eco47III	AGCGCT	1 cut(s)	1601
Eco57I	CTGAAG	6 cut(s)	336, 873, 989, 1527, 2007, 2411
Eco72I	CACGTG	1 cut(s)	2162
EcoNI	CCTNNNNNAGG	1 cut(s)	1917
EcoO109I	RGGNCCY	1 cut(s)	1381
EcoRII	CCWGG	6 cut(s)	177, 486, 1383, 1749, 2040, 2511
EcoT14I	CCWWGG	4 cut(s)	1049, 1352, 2263, 2365
EcoT22I	ATGCAT	2 cut(s)	696, 1976
EcoT38I	GRGCYC	1 cut(s)	600
EgeI	GGCGCC	1 cut(s)	525
EheI	GGCGCC	1 cut(s)	525
ErhI	CCWWGG	4 cut(s)	1049, 1352, 2263, 2365
Esp3I	CGTCTC	1 cut(s)	198
FalI	AAGNNNNNCTT	4 cut(s)	876, 908, 2546, 2578
FaqI	GGGAC	3 cut(s)	181, 1779, 2102
FauI	CCCGC	1 cut(s)	607
FauNDI	CATATG	1 cut(s)	2028
FbaI	TGATCA	2 cut(s)	1132, 2164
FblI	GTMKAC	2 cut(s)	1884, 2613
FokI	GGATG	7 cut(s)	311, 593, 1279, 1733, 2057, 2328, 2528
FriOI	GRGCYC	1 cut(s)	600
FspBI	CTAG	9 cut(s)	14, 270, 938, 1353, 1455, 1775, 2244, 2264, 2788
FspI	TGCGCA	1 cut(s)	1903
GlaI	GCGC	4 cut(s)	525, 1331, 1601, 1903
GsaI	CCCAGC	1 cut(s)	639
GsuI	CTGGAG	4 cut(s)	131, 356, 1772, 2693
HaeII	RGCGCY	3 cut(s)	527, 1333, 1603
HaeIII	GGCC	4 cut(s)	177, 611, 1056, 1382
HapII	CCGG	5 cut(s)	612, 668, 1166, 2009, 2732
HgaI	GACGC	2 cut(s)	127, 2605
HhaI	GCGC	4 cut(s)	526, 1332, 1602, 1904
Hin1I	GRCGYC	3 cut(s)	524, 1768, 2610
Hin6I	GCGC	4 cut(s)	524, 1330, 1600, 1902
HinP1I	GCGC	4 cut(s)	524, 1330, 1600, 1902
HincII	GTYRAC	3 cut(s)	1483, 1837, 2614
HindII	GTYRAC	3 cut(s)	1483, 1837, 2614
HindIII	AAGCTT	2 cut(s)	50, 1001
HpaII	CCGG	5 cut(s)	612, 668, 1166, 2009, 2732
HphI	GGTGA	6 cut(s)	785, 1998, 2030, 2122, 2321, 2335
Hpy166II	GTNNAC	9 cut(s)	1441, 1483, 1837, 1885, 2038, 2063, 2159, 2506, 2614
Hpy188I	TCNGA	9 cut(s)	237, 316, 429, 632, 865, 1732, 2084, 2756, 2768
Hpy188III	TCNNGA	6 cut(s)	95, 110, 326, 782, 1676, 1916
Hpy8I	GTNNAC	9 cut(s)	1441, 1483, 1837, 1885, 2038, 2063, 2159, 2506, 2614
Hpy99I	CGWCG	3 cut(s)	1773, 2615, 2618
HpyCH4III	ACNGT	5 cut(s)	660, 725, 838, 1487, 2122
HpyCH4IV	ACGT	3 cut(s)	1768, 2161, 2610
HpyF3I	CTNAG	5 cut(s)	428, 456, 965, 2633, 2753
HpySE526I	ACGT	3 cut(s)	1768, 2161, 2610
Hsp92I	GRCGYC	3 cut(s)	524, 1768, 2610
HspAI	GCGC	4 cut(s)	524, 1330, 1600, 1902
KasI	GGCGCC	1 cut(s)	523
KpnI	GGTACC	1 cut(s)	2199
Ksp22I	TGATCA	2 cut(s)	1132, 2164
LguI	GCTCTTC	1 cut(s)	824
LmnI	GCTCC	8 cut(s)	407, 490, 649, 907, 934, 1274, 1316, 2188
LweI	GCATC	7 cut(s)	200, 460, 571, 1755, 2205, 2237, 2416
MaeI	CTAG	9 cut(s)	14, 270, 938, 1353, 1455, 1775, 2244, 2264, 2788
MaeII	ACGT	3 cut(s)	1768, 2161, 2610
MaeIII	GTNAC	6 cut(s)	239, 871, 976, 1861, 2143, 2606
MfeI	CAATTG	1 cut(s)	768
MflI	RGATCY	3 cut(s)	1948, 2519, 2768
MhlI	GDGCHC	3 cut(s)	600, 836, 2161
Mly113I	GGCGCC	1 cut(s)	524
MlyI	GAGTC	2 cut(s)	374, 1342
MmeI	TCCRAC	2 cut(s)	62, 2664
Mph1103I	ATGCAT	2 cut(s)	696, 1976
MroXI	GAANNNNTTC	2 cut(s)	321, 1494
MslI	CAYNNNNRTG	3 cut(s)	2043, 2531, 2604
MspCI	CTTAAG	1 cut(s)	1184
MspI	CCGG	5 cut(s)	612, 668, 1166, 2009, 2732
MspR9I	CCNGG	7 cut(s)	179, 488, 1166, 1385, 1751, 2042, 2513
MunI	CAATTG	1 cut(s)	768
Mva1269I	GAATGC	2 cut(s)	423, 1169
MvaI	CCWGG	6 cut(s)	179, 488, 1385, 1751, 2042, 2513
MvnI	CGCG	3 cut(s)	138, 602, 2739
NarI	GGCGCC	1 cut(s)	524
NciI	CCSGG	1 cut(s)	1166
NcoI	CCATGG	1 cut(s)	1049
NdeI	CATATG	1 cut(s)	2028
NlaIV	GGNNCC	6 cut(s)	525, 1055, 1242, 1729, 2197, 2521
NmeAIII	GCCGAG	2 cut(s)	304, 619
NmuCI	GTSAC	2 cut(s)	871, 2606
NsbI	TGCGCA	1 cut(s)	1903
NsiI	ATGCAT	2 cut(s)	696, 1976
NspI	RCATGY	2 cut(s)	1974, 2603
NspV	TTCGAA	1 cut(s)	654
PaeI	GCATGC	2 cut(s)	1974, 2603
PciSI	GCTCTTC	1 cut(s)	824
PctI	GAATGC	2 cut(s)	423, 1169
PdmI	GAANNNNTTC	2 cut(s)	321, 1494
PfeI	GAWTC	9 cut(s)	98, 232, 305, 322, 860, 1090, 1145, 2338, 2585
PflFI	GACNNNGTC	3 cut(s)	1770, 2093, 2459
PflMI	CCANNNNNTGG	2 cut(s)	1963, 2512
PfoI	TCCNGGA	1 cut(s)	1749
Ple19I	CGATCG	1 cut(s)	2101
PleI	GAGTC	2 cut(s)	373, 1342
PluTI	GGCGCC	1 cut(s)	527
PmaCI	CACGTG	1 cut(s)	2162
PmlI	CACGTG	1 cut(s)	2162
PpsI	GAGTC	2 cut(s)	373, 1342
Ppu21I	YACGTR	1 cut(s)	2162
PshBI	ATTAAT	1 cut(s)	1977
Psp6I	CCWGG	6 cut(s)	177, 486, 1383, 1749, 2040, 2511
PspCI	CACGTG	1 cut(s)	2162
PspFI	CCCAGC	1 cut(s)	635
PspGI	CCWGG	6 cut(s)	177, 486, 1383, 1749, 2040, 2511
PspN4I	GGNNCC	6 cut(s)	525, 1055, 1242, 1729, 2197, 2521
PspPI	GGNCC	3 cut(s)	609, 1054, 1381
PstI	CTGCAG	2 cut(s)	146, 2335
PstNI	CAGNNNCTG	2 cut(s)	520, 2434
PsuI	RGATCY	3 cut(s)	1948, 2519, 2768
PsyI	GACNNNGTC	3 cut(s)	1770, 2093, 2459
PvuI	CGATCG	1 cut(s)	2101
RsaI	GTAC	5 cut(s)	485, 1428, 2197, 2382, 2580
RsaNI	GTAC	5 cut(s)	484, 1427, 2196, 2381, 2579
RseI	CAYNNNNRTG	3 cut(s)	2043, 2531, 2604
SalI	GTCGAC	1 cut(s)	2612
SapI	GCTCTTC	1 cut(s)	824
Sau96I	GGNCC	3 cut(s)	609, 1054, 1381
SchI	GAGTC	2 cut(s)	374, 1342
ScrFI	CCNGG	7 cut(s)	179, 488, 1166, 1385, 1751, 2042, 2513
SduI	GDGCHC	3 cut(s)	600, 836, 2161
SfaNI	GCATC	7 cut(s)	200, 460, 571, 1755, 2205, 2237, 2416
SfcI	CTRYAG	3 cut(s)	142, 1626, 2331
SfoI	GGCGCC	1 cut(s)	525
SfuI	TTCGAA	1 cut(s)	654
SgrDI	CGTCGACG	1 cut(s)	2612
SmiMI	CAYNNNNRTG	3 cut(s)	2043, 2531, 2604
SmlI	CTYRAG	3 cut(s)	93, 782, 1184
SmoI	CTYRAG	3 cut(s)	93, 782, 1184
SphI	GCATGC	2 cut(s)	1974, 2603
SsiI	CCGC	8 cut(s)	600, 665, 1151, 1667, 1943, 2226, 2392, 2739
SspDI	GGCGCC	1 cut(s)	523
SspMI	CTAG	9 cut(s)	14, 270, 938, 1353, 1455, 1775, 2244, 2264, 2788
StyD4I	CCNGG	7 cut(s)	177, 486, 1164, 1383, 1749, 2040, 2511
StyI	CCWWGG	4 cut(s)	1049, 1352, 2263, 2365
TaaI	ACNGT	5 cut(s)	660, 725, 838, 1487, 2122
TaiI	ACGT	3 cut(s)	1771, 2164, 2613
TaqI	TCGA	9 cut(s)	101, 654, 1084, 1346, 1633, 1858, 2097, 2350, 2613
TatI	WGTACW	2 cut(s)	2380, 2578
TauI	GCSGC	2 cut(s)	667, 2228
TfiI	GAWTC	9 cut(s)	98, 232, 305, 322, 860, 1090, 1145, 2338, 2585
TscAI	CASTG	3 cut(s)	730, 841, 1990
TseFI	GTSAC	2 cut(s)	871, 2606
Tsp45I	GTSAC	2 cut(s)	871, 2606
TspDTI	ATGAA	5 cut(s)	894, 1655, 2040, 2577, 2636
TspGWI	ACGGA	3 cut(s)	311, 1673, 1886
TspRI	CASTG	3 cut(s)	730, 841, 1990
Tth111I	GACNNNGTC	3 cut(s)	1770, 2093, 2459
Van91I	CCANNNNNTGG	2 cut(s)	1963, 2512
Vha464I	CTTAAG	1 cut(s)	1184
VneI	GTGCAC	1 cut(s)	2157
VspI	ATTAAT	1 cut(s)	1977
XagI	CCTNNNNNAGG	1 cut(s)	1917
XapI	RAATTY	5 cut(s)	529, 1816, 1890, 2566, 2677
XceI	RCATGY	2 cut(s)	1974, 2603
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2513
XmaJI	CCTAGG	2 cut(s)	1352, 2263
XmiI	GTMKAC	2 cut(s)	1884, 2613
XmnI	GAANNNNTTC	2 cut(s)	321, 1494
XspI	CTAG	9 cut(s)	14, 270, 938, 1353, 1455, 1775, 2244, 2264, 2788
ZraI	GACGTC	2 cut(s)	1769, 2611
Zsp2I	ATGCAT	2 cut(s)	696, 1976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.