Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012825

Physical Location & Seq

Genomic Coordinates Reverse (-)

4341801 .. 4345235

Gene Structure 3435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1062000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3435 bp

ATGAAATGCCATCTGCATTTCAAAACCGCGCTTTTTCTCAACGTCTTCTGCTTATGGAGTAGCGTTTCTGGGGAGGCGCTGTTTTCTGAGAAGTCGATTTTGCTCGAGATCAAGAGCTCGGTTTCCGACCCGGATGGGGTTCTCTTCGGCTGGAGGTCCAACAGCTTCGATCACCACTGTTCGTGGATCGGGGTCTCATGCGACACCAAATCGAGGGTCATTTCGCTCAGTCTCGCCGGCAGCGGTGCCAAAGGAGGTAATTTTCGAGCTCTGTCTTGCTCTCAGAGTGTGAAATTTCAATTCCCGTACGATGGGTTCGGTGTTTGGAGGAAAATTTTGGTTAGTGGAAAATTGGGTGGAAAGGTTTCGCCTTGGGTGGGAAAGCTCAGTGAGCTTAGAGTTTTATCGCTGCCTTTCAATGAACTTAGTGGCGAAATTCCGAAGGAAATTTGGGGGTTGGAGAAGCTTGAAGTGCTTGATCTTGAGGGGAATTTGTTAAACGGAAATTTGCCAACCCGGTTTTCGGGTTTGAGGACGTTGAGGGTATTGAATCTTGGGTTTAATAGAATTGGTGGGGAGATTCCTTTTTCGCTTTCGAAATGTGTGGGTTTGGAGGTGCTGAATTTGGTAGGTAATGAAGTGAGTGGAACCATCCCTGGGTTTGTTGGTAGGTTTGCAAAGTTGCAGGGGCTTTATTTGGCTCAAAATAGGTTAAATGGGTCTATACCAGCCACATTTGGAAGCTATTGTCGAAATCTTGAATATCTTGATCTGTCGGGAAATTTCCTTGTTGGGAAGATTCCTGGTAGTTTGGGGAATTGTCGGTGGTTACGGACTCTTTTGTTGTTTTCAAATATATTGAATGGTGGCATTCCTCTAGAACTTGGTTGGATTCGAATGCTTGAAGTTCTAGATGTTTCAAGAAATAGCCTTAGTGGCCCAGTCCCTGCTGAGCTTGGACAATGTATCAATTTATCTGTTCTTGTCCTATCAAATCTTTTTAATCCATTGACAACCAATCCGAATACGAATGGAGATACATCAATTGATTTATCTGGTGGTTTGGTGGATGATTACAATTATTACGAAGGCTCCATTCCAGAGGAAATTACAACCCTTCCAAATTTGAAAATAGTTTGGGCTCCAAGGGCAACCCTTGAAGGAAAGCTTCCAAGCAATTGGGGTGGTTGTGAGAACTTGGAAATGTTGAACTTAGCTCAGAACCTTTTCAGCGGAGAAGTCACTGGAGTATTTGAAAGATGCAAGAAGCTGCATTATCTAAATTTGGGATCAAATATGCTGAGTGGGAAGATCGATGAGAAGCTTCCGGTTCCATGTATGACTGTTTTCAATGTCAGTGGGAACTTCATGTCTGGACCTGTCCCCAAGTTTCTGTACAGATTGTGCCCCCAGGTGCCCCCAAATTCAGATCTTGTCCGAGTCAACAATCCATCATTTCCATATCAAGTGCTCTTTACTTGCCAAACTCAGCTTGATAGCCATTTGCCTCTTTCAAGTGGTAGTTTCACTGTGGTTCATGATTTTAGTGGTAACAACTTCACCGGTCCAATCCAACACCTCCCTCTTGCAACTGAGAGACTGGGGAAGCAGACTGTTTATGCATTTCTTGCTGGTGGGAACAAGCTCACGGGATCATTTCCTGAAACCTTACTACAAAAGTGTGATGGATTAGATGGAATGATTATTGATGTTAGCGACAATAAGTTGTCAGGTCAAATTCCTTTTCAGATTGGTGCGATGTGCAGATCTCTTAGATTCCTGGATGCCTCTGGCAATCTTTTATCCAGGTCAATACCTTCAGATATTGGGGATTTGGAATCTCTTGTCTTTCTTGACTTGAGCTGGAACCAGCTGCAGGGTCAAATTCCTGCAGGTATCAGTCATTTGAAGTATCTTAAGTATCTGTCCCTGGATAACAACAACCTCACAGGTGAAATTCCTGCTAGCTTTGTGTGGTTGCACTCCCTTGAAGTTCTAAAGCTGTCTTCAAATTCTCTTTCTGGGGACATTCCGCAAGGTCTTGTGAACTTGAAAAATATAACTGTTTTTCTGCTCGACAACAACAAACTCTCTGGGCACATCCCTTCAGGCTTGACTAATCTGAAATCCCTATCTACATTCAATGCCTCCTTCAATAACCTGTCTGGTTCATTTCCATTGAACAATAGCATGATGAACTGCAGCAGTGTCCTTGGGAACCCTTTCCTCATTCCATGCCATATTGTTTCGCTAACTGCTCCATCTTCAGATCAGCCAGATAGTTATGGGAGTTCACAACATAACCCTGATTCTCTATCAGCAACTACTGGTGGTGATGATAATAGTGGATTAGGCACAATCGAGATCGCATCCATAGCAGCTGCATCGGCTGTTGTTTTGGTTCTGCTCTCTCTTGTTATCCTGTTCTTTTATATGAGAAAGTGGATACCAGATTCCAGGGTTCAGGGTTTTGAATATAAAGAAATGACTCTGTTTACAGACATTGGGGCTCCATTGACATTTGAGAATATTGTTCAAGCAACAGGGAATTTTAATGGCAGCTACCACATTGGCAGCGGAGGATTTGGGGCCACTTACAAAGCTGAAATTGCTCCAGGAATCACTGTAGCTGTGAAAAGGCTAGCTGTTGGAAGGTTTCACGGTGTTCAACAGTTTCATGCTGAGATTAAAACCCTTGGTAGGGTGAGACACCCAAACCTGGTGACTTTAATAGGTTACCATGCCAGTGAAACTGATATGTTGCTCATATATAATTATTTACCAGGAGGGAATTTGGAAAACTTTATCAAGGAGAGATCTCGGAGGCCTTTTAATTGGCAAATTCTCCACAAGATTGCTTTGCATATAGCCCGTGCACTTGCTTATCTGCATGGCGAGTGTATTCCGCGTGTCCTACACCGTGATGTCAAGCCAAGTAACATACTGTTGGATGATGAGTTCAATGCTTACTTGTCTGACTTTGGATTATCTAGGCTTTTGGGAACTTCAGAAACACACGCAACAACTGGTCCAGCAGGGACTTTTGGGTACGTAGCACCAGAGTATGCGATGACTTGCCATGTGTCTGAAAAGTCTGATGTTTACAGTTACGGTGTGGTGCTGCTTGAATTGATGTCGTACAAAAAACCCTTAGACCCTTCGTTTTCATCACATGGCGATGGTTTCAACATTGTCTCTTGGGTGGACATGCTGTTAGAAAGGGGTCAGGATAAGGAGGTCTTCGTAGAAGGGCTATGGGATGCAGGACCCCAGAATGATCTAGTCGAGATGTTGAATTTGGCTGTCGCGTGCACAGTCAAGACCCTCGCCTCTAGGCCAACGATGAAGCAAGTCGTCGAAAGATTAAAGCGAATCCAACCTATTTCAAGGAAGGCGGATAACGAGCAAAGTATAGAGCTAGCGATCATAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1145

Amino Acids

125.05

Weight (kDa)

6.29

Isoelectric Point (pI)

34.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 68	6.9e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	126 - 229	1.8e-07	Leucine-rich repeat region
LRR_8	PF13855	227 - 285	2.6e-06	Leucine rich repeat
LRR_14	PF23598	633 - 714	7.8e-08	Leucine-rich repeat region
LRR_8	PF13855	634 - 673	1e-05	Leucine rich repeat
Pkinase	PF00069	855 - 1120	2.9e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	856 - 1122	2.3e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69270
fragaria_vesca	FvH4_4g29970
malus_domestica	MD13G1062000.v1.1 MD16G1060500.v1.1
prunus_persica	Prupe.1G287800_v2.0.a1
pyrus_communis	pycom13g05450 pycom16g05270
rosa_chinensis	RchiOBHm_Chr4g0438241
rosa_laevigata	RLG00000006378
rosa_multiflora	Rmu_sc0002682.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379330
rosa_rugosa	Rorug04G0308300
rosa_samantha	Rh4AG359300 Rh4BG370100 Rh4CG384300 Rh4DG365200
rosa_wichuraiana	Rw0G006390 Rw0G016330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1883
AccB1I	GGYRCC	2 cut(s)	245, 1414
AccII	CGCG	3 cut(s)	29, 2908, 3308
AciI	CCGC	7 cut(s)	27, 243, 1233, 2033, 2577, 2906, 3395
AclWI	GGATC	3 cut(s)	194, 1297, 1660
AcuI	CTGAAG	4 cut(s)	1803, 2091, 2250, 2991
AfaI	GTAC	4 cut(s)	308, 1397, 3050, 3140
AflII	CTTAAG	1 cut(s)	1916
AgeI	ACCGGT	1 cut(s)	1562
AjuI	GAANNNNNNNTTGG	2 cut(s)	505, 537
AloI	GAACNNNNNNTCC	2 cut(s)	2942, 2974
Alw21I	GWGCWC	5 cut(s)	119, 271, 1473, 2878, 3314
Alw26I	GTCTC	5 cut(s)	199, 236, 1591, 2701, 3199
Alw44I	GTGCAC	2 cut(s)	2874, 3310
AlwI	GGATC	3 cut(s)	194, 1297, 1660
AlwNI	CAGNNNCTG	1 cut(s)	947
Ama87I	CYCGRG	1 cut(s)	104
AoxI	GGCC	4 cut(s)	937, 2589, 2825, 3335
ApaLI	GTGCAC	2 cut(s)	2874, 3310
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
AsiGI	ACCGGT	1 cut(s)	1562
Asp700I	GAANNNNTTC	3 cut(s)	364, 1226, 2222
AspLEI	GCGC	2 cut(s)	31, 79
AspS9I	GGNCC	7 cut(s)	156, 938, 1376, 1565, 2589, 3029, 3266
AsuC2I	CCSGG	2 cut(s)	131, 517
AsuHPI	GGTGA	6 cut(s)	164, 1552, 1964, 2345, 2716, 2734
AsuII	TTCGAA	2 cut(s)	596, 895
AsuNHI	GCTAGC	3 cut(s)	1964, 2641, 3418
AvaI	CYCGRG	1 cut(s)	104
AvaII	GGWCC	5 cut(s)	156, 1376, 1565, 3029, 3266
BaeGI	GKGCMC	5 cut(s)	1409, 1419, 2100, 2878, 3314
BanI	GGYRCC	2 cut(s)	245, 1414
BanII	GRGCYC	4 cut(s)	119, 271, 1144, 2512
BbsI	GAAGAC	3 cut(s)	37, 1998, 3232
Bbv12I	GWGCWC	5 cut(s)	119, 271, 1473, 2878, 3314
BccI	CCATC	9 cut(s)	18, 128, 305, 659, 1459, 1679, 1688, 2269, 3173
BcgI	CGANNNNNNTGC	2 cut(s)	2367, 2401
BciVI	GTATCC	1 cut(s)	2439
BcnI	CCSGG	2 cut(s)	131, 517
BcoDI	GTCTC	5 cut(s)	199, 236, 1591, 2701, 3199
BfaI	CTAG	9 cut(s)	878, 911, 1965, 2642, 2991, 3281, 3333, 3419, 3433
BfmI	CTRYAG	4 cut(s)	1874, 1890, 2200, 2625
BfoI	RGCGCY	1 cut(s)	80
BfrI	CTTAAG	1 cut(s)	1916
BfuAI	ACCTGC	1 cut(s)	1883
BfuI	GTATCC	1 cut(s)	2439
BglI	GCCNNNNNGGC	1 cut(s)	936
BglII	AGATCT	3 cut(s)	1429, 1766, 2816
BlpI	GCTNAGC	1 cut(s)	951
Bme18I	GGWCC	5 cut(s)	156, 1376, 1565, 3029, 3266
BmeT110I	CYCGRG	1 cut(s)	104
BmgT120I	GGNCC	7 cut(s)	156, 938, 1376, 1565, 2589, 3029, 3266
BmrI	ACTGGG	2 cut(s)	935, 1610
BmsI	GCATC	5 cut(s)	1250, 1774, 2378, 2393, 3250
BmtI	GCTAGC	3 cut(s)	1968, 2645, 3422
BmuI	ACTGGG	2 cut(s)	935, 1610
BpiI	GAAGAC	3 cut(s)	37, 1998, 3232
BpmI	CTGGAG	3 cut(s)	172, 1266, 2598
Bpu1102I	GCTNAGC	1 cut(s)	951
Bpu14I	TTCGAA	2 cut(s)	596, 895
BpuEI	CTTGAG	2 cut(s)	503, 1879
BpuMI	CCSGG	2 cut(s)	131, 517
Bsa29I	ATCGAT	1 cut(s)	1314
BsaAI	YACGTR	1 cut(s)	3052
BsaI	GGTCTC	1 cut(s)	199
BsaJI	CCNNGG	9 cut(s)	371, 655, 656, 1145, 1410, 1929, 2212, 2457, 2695
BsaWI	WCCGGW	2 cut(s)	1327, 1562
BsaXI	ACNNNNNCTCC	4 cut(s)	65, 95, 1076, 1106
Bse118I	RCCGGY	2 cut(s)	236, 1562
Bse1I	ACTGG	6 cut(s)	941, 1249, 1605, 2332, 2745, 3031
BseCI	ATCGAT	1 cut(s)	1314
BseDI	CCNNGG	9 cut(s)	371, 655, 656, 1145, 1410, 1929, 2212, 2457, 2695
BseGI	GGATG	8 cut(s)	139, 651, 1075, 1789, 2100, 2369, 2956, 3265
BseNI	ACTGG	6 cut(s)	941, 1249, 1605, 2332, 2745, 3031
BseSI	GKGCMC	5 cut(s)	1409, 1419, 2100, 2878, 3314
BsgI	GTGCAG	1 cut(s)	1783
Bsh1236I	CGCG	3 cut(s)	29, 2908, 3308
BshFI	GGCC	4 cut(s)	939, 2591, 2827, 3337
BshNI	GGYRCC	2 cut(s)	245, 1414
BshTI	ACCGGT	1 cut(s)	1562
BshVI	ATCGAT	1 cut(s)	1314
BsiHKAI	GWGCWC	5 cut(s)	119, 271, 1473, 2878, 3314
BsiHKCI	CYCGRG	1 cut(s)	104
BsiSI	CCGG	5 cut(s)	131, 237, 517, 1328, 1563
BsiWI	CGTACG	1 cut(s)	306
BslFI	GGGAC	5 cut(s)	929, 1367, 1912, 2039, 3052
BsmAI	GTCTC	5 cut(s)	199, 236, 1591, 2701, 3199
BsmFI	GGGAC	5 cut(s)	929, 1367, 1912, 2039, 3052
BsmI	GAATGC	2 cut(s)	870, 903
BsnI	GGCC	4 cut(s)	939, 2591, 2827, 3337
Bso31I	GGTCTC	1 cut(s)	199
BsoBI	CYCGRG	1 cut(s)	104
Bsp119I	TTCGAA	2 cut(s)	596, 895
Bsp1407I	TGTACA	1 cut(s)	1395
Bsp1720I	GCTNAGC	1 cut(s)	951
BspACI	CCGC	7 cut(s)	27, 243, 1233, 2033, 2577, 2906, 3395
BspANI	GGCC	4 cut(s)	939, 2591, 2827, 3337
BspDI	ATCGAT	1 cut(s)	1314
BspFNI	CGCG	3 cut(s)	29, 2908, 3308
BspHI	TCATGA	1 cut(s)	1537
BspMAI	CTGCAG	3 cut(s)	1878, 1894, 2204
BspMI	ACCTGC	1 cut(s)	1883
BspOI	GCTAGC	3 cut(s)	1968, 2645, 3422
BspPI	GGATC	3 cut(s)	194, 1297, 1660
BspT104I	TTCGAA	2 cut(s)	596, 895
BspT107I	GGYRCC	2 cut(s)	245, 1414
BspTI	CTTAAG	1 cut(s)	1916
BspTNI	GGTCTC	1 cut(s)	199
BsrFI	RCCGGY	2 cut(s)	236, 1562
BsrGI	TGTACA	1 cut(s)	1395
BsrI	ACTGG	6 cut(s)	941, 1249, 1605, 2332, 2745, 3031
BssAI	RCCGGY	2 cut(s)	236, 1562
BssECI	CCNNGG	9 cut(s)	371, 655, 656, 1145, 1410, 1929, 2212, 2457, 2695
BssT1I	CCWWGG	4 cut(s)	371, 1145, 2212, 2695
Bst6I	CTCTTC	1 cut(s)	149
BstAFI	CTTAAG	1 cut(s)	1916
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstAUI	TGTACA	1 cut(s)	1395
BstBAI	YACGTR	1 cut(s)	3052
BstBI	TTCGAA	2 cut(s)	596, 895
BstC8I	GCNNGC	5 cut(s)	238, 1966, 2643, 3310, 3420
BstEII	GGTNACC	1 cut(s)	2735
BstF5I	GGATG	8 cut(s)	139, 651, 1075, 1789, 2100, 2369, 2956, 3265
BstFNI	CGCG	3 cut(s)	29, 2908, 3308
BstH2I	RGCGCY	1 cut(s)	80
BstHHI	GCGC	2 cut(s)	31, 79
BstMAI	GTCTC	5 cut(s)	199, 236, 1591, 2701, 3199
BstMWI	GCNNNNNNNGC	8 cut(s)	391, 472, 936, 1148, 1628, 2375, 2387, 2609
BstNSI	RCATGY	1 cut(s)	3211
BstPI	GGTNACC	1 cut(s)	2735
BstSFI	CTRYAG	4 cut(s)	1874, 1890, 2200, 2625
BstSLI	GKGCMC	5 cut(s)	1409, 1419, 2100, 2878, 3314
BstSNI	TACGTA	1 cut(s)	3052
BstUI	CGCG	3 cut(s)	29, 2908, 3308
BstV2I	GAAGAC	3 cut(s)	37, 1998, 3232
BstX2I	RGATCY	3 cut(s)	1429, 1766, 2816
BstXI	CCANNNNNNTGG	1 cut(s)	1179
BstYI	RGATCY	3 cut(s)	1429, 1766, 2816
Bsu15I	ATCGAT	1 cut(s)	1314
BsuI	GTATCC	1 cut(s)	2439
BsuRI	GGCC	4 cut(s)	939, 2591, 2827, 3337
BsuTUI	ATCGAT	1 cut(s)	1314
BtgZI	GCGATG	3 cut(s)	1772, 3083, 3192
BtsCI	GGATG	8 cut(s)	139, 651, 1075, 1789, 2100, 2369, 2956, 3265
BtsI	GCAGTG	1 cut(s)	2212
BtsIMutI	CAGTG	8 cut(s)	175, 394, 1242, 1363, 1527, 2212, 2622, 2752
BveI	ACCTGC	1 cut(s)	1883
Cac8I	GCNNGC	5 cut(s)	238, 1966, 2643, 3310, 3420
CaiI	CAGNNNCTG	1 cut(s)	947
CciI	TCATGA	1 cut(s)	1537
CfoI	GCGC	2 cut(s)	31, 79
Cfr10I	RCCGGY	2 cut(s)	236, 1562
Cfr13I	GGNCC	7 cut(s)	156, 938, 1376, 1565, 2589, 3029, 3266
ClaI	ATCGAT	1 cut(s)	1314
CsiI	ACCWGGT	1 cut(s)	2718
Csp6I	GTAC	4 cut(s)	307, 1396, 3049, 3139
CspAI	ACCGGT	1 cut(s)	1562
CspCI	CAANNNNNGTGG	4 cut(s)	1165, 1200, 1339, 1374
CviQI	GTAC	4 cut(s)	307, 1396, 3049, 3139
Eam1104I	CTCTTC	1 cut(s)	149
EarI	CTCTTC	1 cut(s)	149
EciI	GGCGGA	1 cut(s)	3410
Ecl136II	GAGCTC	2 cut(s)	117, 269
Eco105I	TACGTA	1 cut(s)	3052
Eco130I	CCWWGG	4 cut(s)	371, 1145, 2212, 2695
Eco147I	AGGCCT	1 cut(s)	2827
Eco24I	GRGCYC	4 cut(s)	119, 271, 1144, 2512
Eco31I	GGTCTC	1 cut(s)	199
Eco47I	GGWCC	5 cut(s)	156, 1376, 1565, 3029, 3266
Eco53kI	GAGCTC	2 cut(s)	117, 269
Eco57I	CTGAAG	4 cut(s)	1803, 2091, 2250, 2991
Eco88I	CYCGRG	1 cut(s)	104
Eco91I	GGTNACC	1 cut(s)	2735
EcoICRI	GAGCTC	2 cut(s)	117, 269
EcoO109I	RGGNCCY	1 cut(s)	3266
EcoO65I	GGTNACC	1 cut(s)	2735
EcoT14I	CCWWGG	4 cut(s)	371, 1145, 2212, 2695
EcoT22I	ATGCAT	1 cut(s)	1624
EcoT38I	GRGCYC	4 cut(s)	119, 271, 1144, 2512
ErhI	CCWWGG	4 cut(s)	371, 1145, 2212, 2695
FalI	AAGNNNNNCTT	4 cut(s)	1152, 1184, 3224, 3256
FaqI	GGGAC	5 cut(s)	929, 1367, 1912, 2039, 3052
FokI	GGATG	8 cut(s)	146, 638, 1082, 1796, 2087, 2356, 2963, 3272
FriOI	GRGCYC	4 cut(s)	119, 271, 1144, 2512
FspBI	CTAG	9 cut(s)	878, 911, 1965, 2642, 2991, 3281, 3333, 3419, 3433
GlaI	GCGC	2 cut(s)	30, 78
GsuI	CTGGAG	3 cut(s)	172, 1266, 2598
HaeII	RGCGCY	1 cut(s)	80
HaeIII	GGCC	4 cut(s)	939, 2591, 2827, 3337
HapII	CCGG	5 cut(s)	131, 237, 517, 1328, 1563
HhaI	GCGC	2 cut(s)	31, 79
Hin6I	GCGC	2 cut(s)	29, 77
HinP1I	GCGC	2 cut(s)	29, 77
HincII	GTYRAC	1 cut(s)	1444
HindII	GTYRAC	1 cut(s)	1444
HindIII	AAGCTT	3 cut(s)	464, 1166, 1322
HpaII	CCGG	5 cut(s)	131, 237, 517, 1328, 1563
HphI	GGTGA	6 cut(s)	164, 1552, 1964, 2345, 2716, 2734
Hpy166II	GTNNAC	8 cut(s)	1444, 2047, 2294, 2496, 2876, 3103, 3205, 3312
Hpy8I	GTNNAC	8 cut(s)	1444, 2047, 2294, 2496, 2876, 3103, 3205, 3312
Hpy99I	CGWCG	1 cut(s)	3359
HpyCH4IV	ACGT	3 cut(s)	42, 536, 3051
HpyF10VI	GCNNNNNNNGC	8 cut(s)	391, 472, 936, 1148, 1628, 2375, 2387, 2609
HpySE526I	ACGT	3 cut(s)	42, 536, 3051
HspAI	GCGC	2 cut(s)	29, 77
KroI	GCCGGC	1 cut(s)	236
KroNI	GCCGGC	1 cut(s)	238
LmnI	GCTCC	5 cut(s)	1097, 1147, 2263, 2515, 2617
LweI	GCATC	5 cut(s)	1250, 1774, 2378, 2393, 3250
MabI	ACCWGGT	1 cut(s)	2718
MaeI	CTAG	9 cut(s)	878, 911, 1965, 2642, 2991, 3281, 3333, 3419, 3433
MaeII	ACGT	3 cut(s)	42, 536, 3051
MaeIII	GTNAC	7 cut(s)	828, 1240, 1550, 2722, 2735, 2936, 3107
MboII	GAAGA	7 cut(s)	37, 136, 808, 1321, 1998, 2256, 3232
MfeI	CAATTG	2 cut(s)	1044, 1177
MflI	RGATCY	3 cut(s)	1429, 1766, 2816
MlyI	GAGTC	3 cut(s)	829, 1449, 2482
MmeI	TCCRAC	8 cut(s)	150, 183, 438, 869, 1597, 2629, 2928, 3400
Mph1103I	ATGCAT	1 cut(s)	1624
MroNI	GCCGGC	1 cut(s)	236
MroXI	GAANNNNTTC	3 cut(s)	364, 1226, 2222
MslI	CAYNNNNRTG	3 cut(s)	2745, 2922, 3177
MspA1I	CMGCKG	5 cut(s)	243, 1233, 1873, 2381, 2577
MspCI	CTTAAG	1 cut(s)	1916
MspI	CCGG	5 cut(s)	131, 237, 517, 1328, 1563
MunI	CAATTG	2 cut(s)	1044, 1177
Mva1269I	GAATGC	2 cut(s)	870, 903
MvnI	CGCG	3 cut(s)	29, 2908, 3308
MwoI	GCNNNNNNNGC	8 cut(s)	391, 472, 936, 1148, 1628, 2375, 2387, 2609
NaeI	GCCGGC	1 cut(s)	238
NciI	CCSGG	2 cut(s)	131, 517
NgoMIV	GCCGGC	1 cut(s)	236
NheI	GCTAGC	3 cut(s)	1964, 2641, 3418
NmuCI	GTSAC	2 cut(s)	1240, 2722
NsiI	ATGCAT	1 cut(s)	1624
NspI	RCATGY	1 cut(s)	3211
NspV	TTCGAA	2 cut(s)	596, 895
PaeR7I	CTCGAG	1 cut(s)	104
PagI	TCATGA	1 cut(s)	1537
PasI	CCCWGGG	1 cut(s)	656
PceI	AGGCCT	1 cut(s)	2827
PctI	GAATGC	2 cut(s)	870, 903
PdiI	GCCGGC	1 cut(s)	238
PdmI	GAANNNNTTC	3 cut(s)	364, 1226, 2222
Pfl23II	CGTACG	1 cut(s)	306
PfoI	TCCNGGA	2 cut(s)	1779, 2614
PinAI	ACCGGT	1 cut(s)	1562
PleI	GAGTC	3 cut(s)	829, 1448, 2482
PpsI	GAGTC	3 cut(s)	829, 1448, 2482
Ppu21I	YACGTR	1 cut(s)	3052
PpuMI	RGGWCCY	1 cut(s)	3266
Psp124BI	GAGCTC	2 cut(s)	119, 271
Psp5II	RGGWCCY	1 cut(s)	3266
PspEI	GGTNACC	1 cut(s)	2735
PspLI	CGTACG	1 cut(s)	306
PspPI	GGNCC	7 cut(s)	156, 938, 1376, 1565, 2589, 3029, 3266
PspPPI	RGGWCCY	1 cut(s)	3266
PsrI	GAACNNNNNNTAC	2 cut(s)	299, 331
PstI	CTGCAG	3 cut(s)	1878, 1894, 2204
PstNI	CAGNNNCTG	1 cut(s)	947
PsuI	RGATCY	3 cut(s)	1429, 1766, 2816
PvuII	CAGCTG	2 cut(s)	1873, 2381
RsaI	GTAC	4 cut(s)	308, 1397, 3050, 3140
RsaNI	GTAC	4 cut(s)	307, 1396, 3049, 3139
RseI	CAYNNNNRTG	3 cut(s)	2745, 2922, 3177
SacI	GAGCTC	2 cut(s)	119, 271
Sau96I	GGNCC	7 cut(s)	156, 938, 1376, 1565, 2589, 3029, 3266
SbfI	CCTGCAGG	1 cut(s)	1894
SchI	GAGTC	3 cut(s)	829, 1449, 2482
SdaI	CCTGCAGG	1 cut(s)	1894
SexAI	ACCWGGT	1 cut(s)	2718
SfaNI	GCATC	5 cut(s)	1250, 1774, 2378, 2393, 3250
SfcI	CTRYAG	4 cut(s)	1874, 1890, 2200, 2625
Sfr274I	CTCGAG	1 cut(s)	104
SfuI	TTCGAA	2 cut(s)	596, 895
SinI	GGWCC	5 cut(s)	156, 1376, 1565, 3029, 3266
SlaI	CTCGAG	1 cut(s)	104
SmiMI	CAYNNNNRTG	3 cut(s)	2745, 2922, 3177
SmlI	CTYRAG	4 cut(s)	104, 482, 1858, 1916
SmoI	CTYRAG	4 cut(s)	104, 482, 1858, 1916
SnaBI	TACGTA	1 cut(s)	3052
Sse8387I	CCTGCAGG	1 cut(s)	1894
SseBI	AGGCCT	1 cut(s)	2827
SsiI	CCGC	7 cut(s)	27, 243, 1233, 2033, 2577, 2906, 3395
SspI	AATATT	1 cut(s)	2530
SspMI	CTAG	9 cut(s)	878, 911, 1965, 2642, 2991, 3281, 3333, 3419, 3433
SstI	GAGCTC	2 cut(s)	119, 271
StuI	AGGCCT	1 cut(s)	2827
StyI	CCWWGG	4 cut(s)	371, 1145, 2212, 2695
TaiI	ACGT	3 cut(s)	45, 539, 3054
TatI	WGTACW	1 cut(s)	1395
TscAI	CASTG	8 cut(s)	182, 394, 1249, 1363, 1534, 2212, 2629, 2752
TseFI	GTSAC	2 cut(s)	1240, 2722
Tsp45I	GTSAC	2 cut(s)	1240, 2722
TspGWI	ACGGA	2 cut(s)	516, 847
TspRI	CASTG	8 cut(s)	182, 394, 1249, 1363, 1534, 2212, 2629, 2752
Vha464I	CTTAAG	1 cut(s)	1916
VneI	GTGCAC	2 cut(s)	2874, 3310
VpaK11BI	GGWCC	5 cut(s)	156, 1376, 1565, 3029, 3266
XbaI	TCTAGA	2 cut(s)	877, 910
XceI	RCATGY	1 cut(s)	3211
XhoI	CTCGAG	1 cut(s)	104
XmnI	GAANNNNTTC	3 cut(s)	364, 1226, 2222
XspI	CTAG	9 cut(s)	878, 911, 1965, 2642, 2991, 3281, 3333, 3419, 3433
Zsp2I	ATGCAT	1 cut(s)	1624

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.