Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012825

Physical Location & Seq

Genomic Coordinates Reverse (-)

61078038 .. 61082184

Gene Structure 4147 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ40638

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3387 bp

ATGAGATGGTGTGGCCTCAGAAACACCCTGTTTCTCCTCAAAGTCTTCTCCTTTCTGTTGTGCTTTTCAGTTTCCGGCGACAAAAACGGCTGGTCGGAGAAGTCGGTTCTGCTCGAGCTGAAGACCTCGGTGTCGGACCCAGATGGGGTTCTGTCGACCTGGGCCCACGGCAGCTCTGATCACCACTGTTCCTGGTTCGGGGTCTCCTGCGACTCGAAATCCAAGGTTGTCTCGCTCAGCGTAGCCGGGAGGAGTGAAAATAAAGGAGGTAACTTTGAAGCTTTTACTTGCTCTGAGACTGTGAAATTTCAATTTCAGTTCGATGGGTTTGGGGCTTGGAGGAGGAGTGGTGAGGTTAGTGGTGGGAAATTAGGTGGAAAGCTTTCACCTTTGGTTGGGAAACTTACTGAGCTTAGAGTTTTATCGCTGCCGTTTAATGAACTTAGTGGTGAGATTCCCGAGGAGGTTTGGGGGTTGGAGAAACTTGAAGTGCTTGATCTTGAGGGTAATTTGTTGGAGGGGAAGTTGCCGAGTTGGTTCTCGGGTTTGAGAAAGTTGAGGGTTTTCAATGTTGGGTTTAATAGGATTGGTGGGGAGGTTCCGGTTTCGCTTTCGAAATGTAGGGATTTGGAGGTCTTGAGTTTAGCTGGAAATGAAGTGAGTGGAGCCATCCCTGTGTTTTTTGGTGGTTTTGCAAAGTTGCAGGGGCTTTATCTGGCTCAAAATAGGTTAAATGGATCTATTCCGGCCAAATTTGGAAGGTCTTGTTTGAATCTTGAGCATTTTGATGTGTCGGGGAATTTCCTTGTTGGGAAGATTCCGCCTACTTTGGGGAGTTGTCTGCGGTTACGGACGCTATTGTTGTTTTCAAATACTCTGGATGGTGGCATTCCTGGTGAACTTGGTAAGATTCGCGGGCTTGAAGTTCTGGATGTGTCTAGAAATAGCCTTAGCGGTCCAATCCCCGCTGAGCTCGGACAGTGTGTCAATTTGTCGATACTTGTCCTGTCGAATCTTTTTAATCCGTTGCCAGTCAATCAGAATGCAAATGGAGATTCATCGGTTAACTCATATAAAGGAGTGGATGATGATTACAATTTTTATGAAGGCTCCATTCCCCAGGAGATTACAAGCTTACCAAATTTGAGAATAGTTTGGGCGCCAAGGGCAACCCTCGAAGGCCAGTTTCCGAGCAATTGGGGTGGTTGTGAGAACTTGGAAATGGTGAACTTGGCTCAGAACCTTTTCACTGGAGAAGTGATTGGAGTGTTTGATAGATGCAAGAAGCTGCAGTATCTGAATTTGAGCTCAAATAATCTGAGTGGGAAGCTTGATGACAAACTCCCAGTTCCTTGTATGACCGTTTTCAGTGTCAGTGGGAACCTCTTGTCTGGACCCATCCCCACATTTAATTTCACAGTGTGCCCCCATGTGCCCCGAAATTCAGATCTTGTCCGCGTCCAGAATCCATCATTTGCATATCAAGTTCTCTTTACTTGCAGAACTTATCTTGACACCCATTTACCACTTTTTGGTGCTAGTTTCACATTGATTCATGATTTTAGTGGTAACAACTTCACTGGTCCAATCCAACACCTTCCTCTTGCACCTGAGAGGTTGGGGAAGCGAACTGTTTATGCATTTCTTGCTGGTGGGAACAAGTTCACTGGATCATTTACTGAAACCTTAAAAGGAAAGTGTGATGGCTTACATGGAATGATATTTAATGTTAGCAACAACAAGTTATCAGGCCGCATTCCTTTCGAGATTGGCATGATGTGCAGATCTCTCAGACTCTTGGATGCCTCTGGAAATCTCTTATCTGGGTCAATACCTCCAGATCTTGGGGATGTGAAGCCTCTTGTCTTTCTTGACTTGAGCAGGAACCAGTTGCAGGGTCAAATTCCCGCAGGTATCAGTAATTTGAAGTATCTCAAGTACCTGTCCTTGGCTAATAACAACCTTACTGGTACGATTCCTGCTAGCTTTGTGGAGTTACGCTCCCTTGAAGTGTTAAAGCTATCTTCAAACTCTCTTTCTGGTGATATCCCGCAAGGTCTTGTTAAGTTGAAAAACCTAACTATTTTTATGCTTGACAACAATAAGCTCTCTGGCCGCATCCCTTCTGGTTTGACTAATGTGAGATCCCTGTCTACATTTAATGTCTCATATAATAACCTGTCTGGTTCATTTCCATTGAACAATAATATGATGAACTGTAGCGGTGTCCTTGGAAACCCTTTCGTTAATCAATGCCGCATTGTTTCCCTCGCTGCACCATACTCAGCTCAGCTGGGTAGCCCTGCCAATTCACCACCAGGTAGTCCTGATTCTCAAGATGGAATAAACTCAATTGAGATTGCATCCATAGTATCTGCATCAGCTATCGTGTTGGTTCTCTTCACTCTTGTTATCCTCTTCTTTTACACAAGAAAATGGATTCCAGATTCCAGAGTTCAGGGTTTTGAAAATAAAGAAATTACAATTTTCACGGAAATTGGGGCACCATTGACGTTTGAGAATATTGTTCAAGCAACAGGGAATTTTAATGCCAGTAACTACATTGGGAGTGGAGGATTTGGGGCAACTTACAAAGCAGAAATTGCTCCAGGAACCATAATAGCTGTGAAGAGGCTTGCCGTTGGAAGGTTTCACGGTGTTCAACAGTTCCATGCTGAGATTAAGACCCTCGGAAGGGTGAGACACCCCAACCTGGTAACTTTAATAGGTTACCATGCCAGTGAAACCGAGATGCTACTCATATATAATTATTTACCAGGAGGGAATTTGGAAAATTTTATTCAGGAAAGATCTAGAAGGTCTTTTACTTGGAAGATTCTCCACAAGATTGCTCTGGATATTGCCCATGCACTTTCTTATCTGCATGACGAGTGTATCCCGCGTGTCCTACACCGTGATGTCAAGCCAAGCAATATACTGTTAGATGATAACTTGAATGCTTATTTGTCTGACTTTGGATTGTCTAGGCTGCTGGGAACTTCTGAAACGCATGCTACAACTGGTGTTGCAGGAACTTTTGGGTACGTAGCACCCGAGTATGCTATGACCTGTCGTGTGTCTGAGAAGGCTGATGTTTACAGTTACGGTGTTGTGCTGCTTGAATTGATTTCTGATAAGAAAGCCCTGGACCCATCATTTTCTTCACATGGAAATGGTTTCAACATTGTTTCTTGGGCGTGCATGCTGTTAAGAATGGGTCGGGCTAAGGACGTATTCATGGAAGGACTATGGGAGGCAGGACCCGGAGATGATCTGGTTGAGATGCTGTACTTGGCTGTCAAATGTACTGTTGAGACCCTCTCCATTAGGCCTACAATGAAGCAAGTTGTCCAAATACTGAAGCGAATACAACCTGCTTCAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1128

Amino Acids

123.02

Weight (kDa)

8.08

Isoelectric Point (pI)

32.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 71	8.3e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	132 - 218	5e-08	Leucine-rich repeat region
LRR_14	PF23598	595 - 721	2.4e-10	Leucine-rich repeat region
LRR_8	PF13855	624 - 679	3.6e-09	Leucine rich repeat
Pkinase	PF00069	851 - 1050	7.2e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	852 - 1048	3.4e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69270
fragaria_vesca	FvH4_4g29970
malus_domestica	MD13G1062000.v1.1 MD16G1060500.v1.1
prunus_persica	Prupe.1G287800_v2.0.a1
pyrus_communis	pycom13g05450 pycom16g05270
rosa_chinensis	RchiOBHm_Chr4g0438241
rosa_laevigata	RLG00000006378
rosa_multiflora	Rmu_sc0002682.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379330
rosa_rugosa	Rorug04G0308300
rosa_samantha	Rh4AG359300 Rh4BG370100 Rh4CG384300 Rh4DG365200
rosa_wichuraiana	Rw0G006390 Rw0G016330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	130
Acc36I	ACCTGC	2 cut(s)	1901, 3382
AccB1I	GGYRCC	2 cut(s)	1159, 2503
AccB7I	CCANNNNNTGG	2 cut(s)	1532, 1844
AccI	GTMKAC	2 cut(s)	155, 2153
AccII	CGCG	3 cut(s)	915, 1458, 2902
AclWI	GGATC	3 cut(s)	745, 1678, 2139
AcoI	YGGCCR	2 cut(s)	747, 2113
AcuI	CTGAAG	2 cut(s)	140, 3380
AcyI	GRCGYC	1 cut(s)	1160
AdeI	CACNNNGTG	1 cut(s)	1422
AfaI	GTAC	5 cut(s)	1940, 1972, 3044, 3290, 3307
AhdI	GACNNNNNGTC	1 cut(s)	983
AjnI	CCWGG	9 cut(s)	158, 191, 892, 1119, 2317, 2608, 2712, 2776, 3144
Alw21I	GWGCWC	2 cut(s)	975, 1310
Alw26I	GTCTC	6 cut(s)	208, 235, 290, 2170, 2695, 3308
AlwI	GGATC	3 cut(s)	745, 1678, 2139
AlwNI	CAGNNNCTG	1 cut(s)	1297
Ama87I	CYCGRG	4 cut(s)	113, 458, 541, 3053
AoxI	GGCC	7 cut(s)	13, 162, 747, 1180, 1750, 2113, 3329
ApaI	GGGCCC	1 cut(s)	166
ApeKI	GCWGC	6 cut(s)	171, 427, 1288, 2273, 2989, 3115
Asp700I	GAANNNNTTC	3 cut(s)	382, 1244, 1661
AspLEI	GCGC	1 cut(s)	1162
AspS9I	GGNCC	8 cut(s)	136, 162, 163, 956, 1394, 1583, 3148, 3260
AsuC2I	CCSGG	2 cut(s)	247, 3264
AsuHPI	GGTGA	9 cut(s)	173, 362, 378, 461, 908, 1237, 2054, 2304, 2710
AsuII	TTCGAA	1 cut(s)	614
AsuNHI	GCTAGC	1 cut(s)	1982
AvaI	CYCGRG	4 cut(s)	113, 458, 541, 3053
AvaII	GGWCC	6 cut(s)	136, 956, 1394, 1583, 3148, 3260
BaeGI	GKGCMC	4 cut(s)	166, 1427, 1437, 2506
BaeI	ACNNNNGTAYC	2 cut(s)	2878, 2911
BanI	GGYRCC	2 cut(s)	1159, 2503
BanII	GRGCYC	3 cut(s)	166, 975, 1310
BarI	GAAGNNNNNNTAC	2 cut(s)	2809, 2841
BbsI	GAAGAC	2 cut(s)	37, 128
Bbv12I	GWGCWC	2 cut(s)	975, 1310
BbvI	GCAGC	6 cut(s)	183, 414, 1275, 2260, 2976, 3102
BccI	CCATC	9 cut(s)	137, 317, 677, 875, 1406, 1477, 1697, 2333, 3160
BceAI	ACGGC	4 cut(s)	103, 184, 415, 2624
BcgI	CGANNNNNNTGC	2 cut(s)	1744, 1778
BciT130I	CCWGG	9 cut(s)	160, 193, 894, 1121, 2319, 2610, 2714, 2778, 3146
BciVI	GTATCC	1 cut(s)	2906
BclI	TGATCA	1 cut(s)	178
BcnI	CCSGG	2 cut(s)	247, 3264
BcoDI	GTCTC	6 cut(s)	208, 235, 290, 2170, 2695, 3308
BfaI	CTAG	6 cut(s)	939, 1539, 1983, 2814, 2985, 3385
BfmI	CTRYAG	2 cut(s)	1289, 2218
BfoI	RGCGCY	1 cut(s)	1163
BfuAI	ACCTGC	2 cut(s)	1901, 3382
BfuI	GTATCC	1 cut(s)	2906
BglII	AGATCT	4 cut(s)	1447, 1784, 1840, 2810
BisI	GCNGC	9 cut(s)	172, 428, 1289, 1753, 2116, 2257, 2274, 2990, 3116
BlpI	GCTNAGC	3 cut(s)	236, 969, 2289
BlsI	GCNGC	9 cut(s)	173, 429, 1290, 1754, 2117, 2258, 2275, 2991, 3117
Bme18I	GGWCC	6 cut(s)	136, 956, 1394, 1583, 3148, 3260
BmeRI	GACNNNNNGTC	1 cut(s)	983
BmeT110I	CYCGRG	4 cut(s)	113, 458, 541, 3053
BmgT120I	GGNCC	8 cut(s)	136, 162, 163, 956, 1394, 1583, 3148, 3260
BmrI	ACTGGG	1 cut(s)	1340
BmsI	GCATC	7 cut(s)	1268, 1792, 2127, 2372, 2387, 2742, 3273
BmtI	GCTAGC	1 cut(s)	1986
BmuI	ACTGGG	1 cut(s)	1340
BpiI	GAAGAC	2 cut(s)	37, 128
BplI	GAGNNNNNCTC	4 cut(s)	1985, 2017, 3305, 3337
BpmI	CTGGAG	3 cut(s)	1272, 1821, 2592
Bpu10I	CCTNAGC	2 cut(s)	950, 3225
Bpu1102I	GCTNAGC	3 cut(s)	236, 969, 2289
Bpu14I	TTCGAA	1 cut(s)	614
BpuEI	CTTGAG	6 cut(s)	521, 658, 797, 1897, 1919, 2319
BpuMI	CCSGG	2 cut(s)	247, 3264
BsaAI	YACGTR	1 cut(s)	3046
BsaHI	GRCGYC	1 cut(s)	1160
BsaI	GGTCTC	2 cut(s)	208, 3308
BsaWI	WCCGGW	1 cut(s)	601
BsaXI	ACNNNNNCTCC	6 cut(s)	331, 334, 361, 364, 509, 539
BseBI	CCWGG	9 cut(s)	160, 193, 894, 1121, 2319, 2610, 2714, 2778, 3146
BseGI	GGATG	9 cut(s)	669, 886, 937, 1090, 1398, 1807, 1855, 2118, 2363
BseRI	GAGGAG	5 cut(s)	26, 265, 355, 358, 476
BseSI	GKGCMC	4 cut(s)	166, 1427, 1437, 2506
BseXI	GCAGC	6 cut(s)	183, 414, 1275, 2260, 2976, 3102
BseYI	CCCAGC	2 cut(s)	2293, 2992
BsgI	GTGCAG	2 cut(s)	1801, 2259
Bsh1236I	CGCG	3 cut(s)	915, 1458, 2902
BshFI	GGCC	7 cut(s)	15, 164, 749, 1182, 1752, 2115, 3331
BshNI	GGYRCC	2 cut(s)	1159, 2503
BsiHKAI	GWGCWC	2 cut(s)	975, 1310
BsiHKCI	CYCGRG	4 cut(s)	113, 458, 541, 3053
BsiSI	CCGG	5 cut(s)	75, 246, 602, 746, 3264
BsmAI	GTCTC	6 cut(s)	208, 235, 290, 2170, 2695, 3308
BsmI	GAATGC	4 cut(s)	888, 1048, 1755, 2962
BsnI	GGCC	7 cut(s)	15, 164, 749, 1182, 1752, 2115, 3331
Bso31I	GGTCTC	2 cut(s)	208, 3308
BsoBI	CYCGRG	4 cut(s)	113, 458, 541, 3053
Bsp119I	TTCGAA	1 cut(s)	614
Bsp120I	GGGCCC	1 cut(s)	162
Bsp1286I	GDGCHC	6 cut(s)	166, 975, 1310, 1427, 1437, 2506
Bsp1720I	GCTNAGC	3 cut(s)	236, 969, 2289
BspANI	GGCC	7 cut(s)	15, 164, 749, 1182, 1752, 2115, 3331
BspFNI	CGCG	3 cut(s)	915, 1458, 2902
BspHI	TCATGA	1 cut(s)	1555
BspMAI	CTGCAG	1 cut(s)	1293
BspMI	ACCTGC	2 cut(s)	1901, 3382
BspOI	GCTAGC	1 cut(s)	1986
BspPI	GGATC	3 cut(s)	745, 1678, 2139
BspT104I	TTCGAA	1 cut(s)	614
BspT107I	GGYRCC	2 cut(s)	1159, 2503
BspTNI	GGTCTC	2 cut(s)	208, 3308
BssNI	GRCGYC	1 cut(s)	1160
BssT1I	CCWWGG	4 cut(s)	222, 1163, 1947, 2230
Bst2UI	CCWGG	9 cut(s)	160, 193, 894, 1121, 2319, 2610, 2714, 2778, 3146
Bst6I	CTCTTC	3 cut(s)	2405, 2423, 2624
BstACI	GRCGYC	1 cut(s)	1160
BstAPI	GCANNNNNTGC	3 cut(s)	1646, 1779, 2603
BstBAI	YACGTR	1 cut(s)	3046
BstBI	TTCGAA	1 cut(s)	614
BstC8I	GCNNGC	6 cut(s)	917, 1984, 2637, 3012, 3199, 3203
BstDSI	CCRYGG	1 cut(s)	166
BstEII	GGTNACC	1 cut(s)	2729
BstF5I	GGATG	9 cut(s)	669, 886, 937, 1090, 1398, 1807, 1855, 2118, 2363
BstFNI	CGCG	3 cut(s)	915, 1458, 2902
BstH2I	RGCGCY	1 cut(s)	1163
BstHHI	GCGC	1 cut(s)	1162
BstMAI	GTCTC	6 cut(s)	208, 235, 290, 2170, 2695, 3308
BstMWI	GCNNNNNNNGC	4 cut(s)	1166, 1646, 1779, 2603
BstNI	CCWGG	9 cut(s)	160, 193, 894, 1121, 2319, 2610, 2714, 2778, 3146
BstNSI	RCATGY	2 cut(s)	3014, 3205
BstPI	GGTNACC	1 cut(s)	2729
BstSFI	CTRYAG	2 cut(s)	1289, 2218
BstSLI	GKGCMC	4 cut(s)	166, 1427, 1437, 2506
BstSNI	TACGTA	1 cut(s)	3046
BstUI	CGCG	3 cut(s)	915, 1458, 2902
BstV1I	GCAGC	6 cut(s)	183, 414, 1275, 2260, 2976, 3102
BstV2I	GAAGAC	2 cut(s)	37, 128
BstX2I	RGATCY	6 cut(s)	737, 1447, 1784, 1840, 2144, 2810
BstYI	RGATCY	6 cut(s)	737, 1447, 1784, 1840, 2144, 2810
BsuI	GTATCC	1 cut(s)	2906
BsuRI	GGCC	7 cut(s)	15, 164, 749, 1182, 1752, 2115, 3331
BtgI	CCRYGG	1 cut(s)	166
BtsCI	GGATG	9 cut(s)	669, 886, 937, 1090, 1398, 1807, 1855, 2118, 2363
BtsIMutI	CAGTG	9 cut(s)	184, 986, 1248, 1375, 1381, 1425, 1578, 1665, 2746
BveI	ACCTGC	2 cut(s)	1901, 3382
Cac8I	GCNNGC	6 cut(s)	917, 1984, 2637, 3012, 3199, 3203
CaiI	CAGNNNCTG	1 cut(s)	1297
CciI	TCATGA	1 cut(s)	1555
CfoI	GCGC	1 cut(s)	1162
Cfr13I	GGNCC	8 cut(s)	136, 162, 163, 956, 1394, 1583, 3148, 3260
CseI	GACGC	2 cut(s)	862, 1447
CsiI	ACCWGGT	2 cut(s)	2317, 2712
Csp6I	GTAC	5 cut(s)	1939, 1971, 3043, 3289, 3306
CspCI	CAANNNNNGTGG	2 cut(s)	1183, 1218
CviQI	GTAC	5 cut(s)	1939, 1971, 3043, 3289, 3306
DinI	GGCGCC	1 cut(s)	1161
DraIII	CACNNNGTG	1 cut(s)	1422
DrdI	GACNNNNNNGTC	1 cut(s)	130
DriI	GACNNNNNGTC	1 cut(s)	983
DseDI	GACNNNNNNGTC	1 cut(s)	130
EaeI	YGGCCR	2 cut(s)	747, 2113
Eam1104I	CTCTTC	3 cut(s)	2405, 2423, 2624
Eam1105I	GACNNNNNGTC	1 cut(s)	983
EarI	CTCTTC	3 cut(s)	2405, 2423, 2624
EciI	GGCGGA	1 cut(s)	810
Ecl136II	GAGCTC	2 cut(s)	973, 1308
Eco105I	TACGTA	1 cut(s)	3046
Eco130I	CCWWGG	4 cut(s)	222, 1163, 1947, 2230
Eco147I	AGGCCT	1 cut(s)	3331
Eco24I	GRGCYC	3 cut(s)	166, 975, 1310
Eco31I	GGTCTC	2 cut(s)	208, 3308
Eco32I	GATATC	1 cut(s)	2047
Eco47I	GGWCC	6 cut(s)	136, 956, 1394, 1583, 3148, 3260
Eco53kI	GAGCTC	2 cut(s)	973, 1308
Eco57I	CTGAAG	2 cut(s)	140, 3380
Eco88I	CYCGRG	4 cut(s)	113, 458, 541, 3053
Eco91I	GGTNACC	1 cut(s)	2729
EcoICRI	GAGCTC	2 cut(s)	973, 1308
EcoO109I	RGGNCCY	1 cut(s)	3260
EcoO65I	GGTNACC	1 cut(s)	2729
EcoRII	CCWGG	9 cut(s)	158, 191, 892, 1119, 2317, 2608, 2712, 2776, 3144
EcoRV	GATATC	1 cut(s)	2047
EcoT14I	CCWWGG	4 cut(s)	222, 1163, 1947, 2230
EcoT22I	ATGCAT	1 cut(s)	1642
EcoT38I	GRGCYC	3 cut(s)	166, 975, 1310
EgeI	GGCGCC	1 cut(s)	1161
EheI	GGCGCC	1 cut(s)	1161
ErhI	CCWWGG	4 cut(s)	222, 1163, 1947, 2230
FauI	CCCGC	5 cut(s)	908, 973, 1915, 2058, 2907
FbaI	TGATCA	1 cut(s)	178
FblI	GTMKAC	2 cut(s)	155, 2153
Fnu4HI	GCNGC	9 cut(s)	172, 428, 1289, 1753, 2116, 2257, 2274, 2990, 3116
FokI	GGATG	9 cut(s)	656, 893, 944, 1097, 1385, 1814, 1862, 2105, 2350
FriOI	GRGCYC	3 cut(s)	166, 975, 1310
Fsp4HI	GCNGC	9 cut(s)	172, 428, 1289, 1753, 2116, 2257, 2274, 2990, 3116
FspBI	CTAG	6 cut(s)	939, 1539, 1983, 2814, 2985, 3385
GlaI	GCGC	1 cut(s)	1161
GluI	GCNGC	9 cut(s)	172, 428, 1289, 1753, 2116, 2257, 2274, 2990, 3116
GsaI	CCCAGC	2 cut(s)	2297, 2996
GsuI	CTGGAG	3 cut(s)	1272, 1821, 2592
HaeII	RGCGCY	1 cut(s)	1163
HaeIII	GGCC	7 cut(s)	15, 164, 749, 1182, 1752, 2115, 3331
HapII	CCGG	5 cut(s)	75, 246, 602, 746, 3264
HgaI	GACGC	2 cut(s)	862, 1447
HhaI	GCGC	1 cut(s)	1162
Hin1I	GRCGYC	1 cut(s)	1160
Hin6I	GCGC	1 cut(s)	1160
HinP1I	GCGC	1 cut(s)	1160
HincII	GTYRAC	2 cut(s)	156, 1066
HindII	GTYRAC	2 cut(s)	156, 1066
HindIII	AAGCTT	4 cut(s)	279, 380, 1132, 1328
HpaI	GTTAAC	1 cut(s)	1066
HpaII	CCGG	5 cut(s)	75, 246, 602, 746, 3264
HphI	GGTGA	9 cut(s)	173, 362, 378, 461, 908, 1237, 2054, 2304, 2710
Hpy166II	GTNNAC	7 cut(s)	156, 899, 1066, 1228, 1665, 2154, 3097
Hpy8I	GTNNAC	7 cut(s)	156, 899, 1066, 1228, 1665, 2154, 3097
HpyCH4IV	ACGT	3 cut(s)	2513, 3045, 3231
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1166, 1646, 1779, 2603
HpySE526I	ACGT	3 cut(s)	2513, 3045, 3231
Hsp92I	GRCGYC	1 cut(s)	1160
HspAI	GCGC	1 cut(s)	1160
KasI	GGCGCC	1 cut(s)	1159
Ksp22I	TGATCA	1 cut(s)	178
KspAI	GTTAAC	1 cut(s)	1066
LmnI	GCTCC	4 cut(s)	665, 1115, 2006, 2611
Lsp1109I	GCAGC	6 cut(s)	183, 414, 1275, 2260, 2976, 3102
LweI	GCATC	7 cut(s)	1268, 1792, 2127, 2372, 2387, 2742, 3273
MabI	ACCWGGT	2 cut(s)	2317, 2712
MaeI	CTAG	6 cut(s)	939, 1539, 1983, 2814, 2985, 3385
MaeII	ACGT	3 cut(s)	2513, 3045, 3231
MaeIII	GTNAC	8 cut(s)	269, 846, 1568, 1995, 2555, 2716, 2729, 3101
MboII	GAAGA	9 cut(s)	37, 133, 826, 2016, 2392, 2410, 2641, 2845, 3153
MfeI	CAATTG	2 cut(s)	1195, 2352
MflI	RGATCY	6 cut(s)	737, 1447, 1784, 1840, 2144, 2810
MhlI	GDGCHC	6 cut(s)	166, 975, 1310, 1427, 1437, 2506
Mly113I	GGCGCC	1 cut(s)	1160
MlyI	GAGTC	2 cut(s)	206, 1788
MmeI	TCCRAC	6 cut(s)	75, 114, 456, 495, 1615, 2623
Mph1103I	ATGCAT	1 cut(s)	1642
MroXI	GAANNNNTTC	3 cut(s)	382, 1244, 1661
MslI	CAYNNNNRTG	5 cut(s)	674, 786, 2739, 2916, 3171
MspA1I	CMGCKG	2 cut(s)	968, 2293
MspI	CCGG	5 cut(s)	75, 246, 602, 746, 3264
MunI	CAATTG	2 cut(s)	1195, 2352
Mva1269I	GAATGC	4 cut(s)	888, 1048, 1755, 2962
MvaI	CCWGG	9 cut(s)	160, 193, 894, 1121, 2319, 2610, 2714, 2778, 3146
MvnI	CGCG	3 cut(s)	915, 1458, 2902
MwoI	GCNNNNNNNGC	4 cut(s)	1166, 1646, 1779, 2603
NarI	GGCGCC	1 cut(s)	1160
NciI	CCSGG	2 cut(s)	247, 3264
NheI	GCTAGC	1 cut(s)	1982
NmeAIII	GCCGAG	1 cut(s)	555
NsiI	ATGCAT	1 cut(s)	1642
NspI	RCATGY	2 cut(s)	3014, 3205
NspV	TTCGAA	1 cut(s)	614
PaeI	GCATGC	2 cut(s)	3014, 3205
PaeR7I	CTCGAG	1 cut(s)	113
PagI	TCATGA	1 cut(s)	1555
PceI	AGGCCT	1 cut(s)	3331
PcsI	WCGNNNNNNNCGW	1 cut(s)	3051
PctI	GAATGC	4 cut(s)	888, 1048, 1755, 2962
PdmI	GAANNNNTTC	3 cut(s)	382, 1244, 1661
PflMI	CCANNNNNTGG	2 cut(s)	1532, 1844
PfoI	TCCNGGA	1 cut(s)	2608
PkrI	GCNGC	9 cut(s)	173, 429, 1290, 1754, 2117, 2258, 2275, 2991, 3117
PleI	GAGTC	2 cut(s)	206, 1788
PluTI	GGCGCC	1 cut(s)	1163
PpsI	GAGTC	2 cut(s)	206, 1788
Ppu21I	YACGTR	1 cut(s)	3046
PpuMI	RGGWCCY	1 cut(s)	3260
Psp124BI	GAGCTC	2 cut(s)	975, 1310
Psp5II	RGGWCCY	1 cut(s)	3260
Psp6I	CCWGG	9 cut(s)	158, 191, 892, 1119, 2317, 2608, 2712, 2776, 3144
PspEI	GGTNACC	1 cut(s)	2729
PspFI	CCCAGC	2 cut(s)	2293, 2992
PspGI	CCWGG	9 cut(s)	158, 191, 892, 1119, 2317, 2608, 2712, 2776, 3144
PspOMI	GGGCCC	1 cut(s)	162
PspPI	GGNCC	8 cut(s)	136, 162, 163, 956, 1394, 1583, 3148, 3260
PspPPI	RGGWCCY	1 cut(s)	3260
PspXI	VCTCGAGB	1 cut(s)	113
PstI	CTGCAG	1 cut(s)	1293
PstNI	CAGNNNCTG	1 cut(s)	1297
PsuI	RGATCY	6 cut(s)	737, 1447, 1784, 1840, 2144, 2810
PvuII	CAGCTG	1 cut(s)	2293
RsaI	GTAC	5 cut(s)	1940, 1972, 3044, 3290, 3307
RsaNI	GTAC	5 cut(s)	1939, 1971, 3043, 3289, 3306
RseI	CAYNNNNRTG	5 cut(s)	674, 786, 2739, 2916, 3171
SacI	GAGCTC	2 cut(s)	975, 1310
SalI	GTCGAC	1 cut(s)	154
SatI	GCNGC	9 cut(s)	172, 428, 1289, 1753, 2116, 2257, 2274, 2990, 3116
Sau96I	GGNCC	8 cut(s)	136, 162, 163, 956, 1394, 1583, 3148, 3260
SchI	GAGTC	2 cut(s)	206, 1788
SduI	GDGCHC	6 cut(s)	166, 975, 1310, 1427, 1437, 2506
SexAI	ACCWGGT	2 cut(s)	2317, 2712
SfaNI	GCATC	7 cut(s)	1268, 1792, 2127, 2372, 2387, 2742, 3273
SfcI	CTRYAG	2 cut(s)	1289, 2218
SfoI	GGCGCC	1 cut(s)	1161
Sfr274I	CTCGAG	1 cut(s)	113
SfuI	TTCGAA	1 cut(s)	614
SinI	GGWCC	6 cut(s)	136, 956, 1394, 1583, 3148, 3260
SlaI	CTCGAG	1 cut(s)	113
SmiMI	CAYNNNNRTG	5 cut(s)	674, 786, 2739, 2916, 3171
SmlI	CTYRAG	7 cut(s)	113, 500, 637, 776, 1876, 1934, 2334
SmoI	CTYRAG	7 cut(s)	113, 500, 637, 776, 1876, 1934, 2334
SnaBI	TACGTA	1 cut(s)	3046
SphI	GCATGC	2 cut(s)	3014, 3205
SseBI	AGGCCT	1 cut(s)	3331
SspDI	GGCGCC	1 cut(s)	1159
SspI	AATATT	1 cut(s)	2524
SspMI	CTAG	6 cut(s)	939, 1539, 1983, 2814, 2985, 3385
SstI	GAGCTC	2 cut(s)	975, 1310
StuI	AGGCCT	1 cut(s)	3331
StyI	CCWWGG	4 cut(s)	222, 1163, 1947, 2230
TaiI	ACGT	3 cut(s)	2516, 3048, 3234
TaqI	TCGA	9 cut(s)	114, 155, 215, 321, 614, 995, 1010, 1176, 1764
TatI	WGTACW	2 cut(s)	3288, 3305
TauI	GCSGC	3 cut(s)	1755, 2118, 2259
TscAI	CASTG	9 cut(s)	191, 986, 1255, 1375, 1381, 1425, 1585, 1672, 2746
TseI	GCWGC	6 cut(s)	171, 427, 1288, 2273, 2989, 3115
TspDTI	ATGAA	9 cut(s)	453, 669, 1047, 1119, 1544, 2178, 2228, 3226, 3353
TspGWI	ACGGA	3 cut(s)	865, 1014, 2507
TspRI	CASTG	9 cut(s)	191, 986, 1255, 1375, 1381, 1425, 1585, 1672, 2746
Van91I	CCANNNNNTGG	2 cut(s)	1532, 1844
VpaK11BI	GGWCC	6 cut(s)	136, 956, 1394, 1583, 3148, 3260
XbaI	TCTAGA	2 cut(s)	938, 2813
XceI	RCATGY	2 cut(s)	3014, 3205
XhoI	CTCGAG	1 cut(s)	113
XmiI	GTMKAC	2 cut(s)	155, 2153
XmnI	GAANNNNTTC	3 cut(s)	382, 1244, 1661
XspI	CTAG	6 cut(s)	939, 1539, 1983, 2814, 2985, 3385
Zsp2I	ATGCAT	1 cut(s)	1642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.