Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012825

Physical Location & Seq

Genomic Coordinates Reverse (-)

4338744 .. 4342106

Gene Structure 3363 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1060500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3363 bp

ATGAAATGCCATATGCTTTTCAAAACCGCGCTTTTTCTCAACGCCTTCTGCTTATTGAGTTGCGTTTCTGGGGAAGCGCCGGTTTCTGAGAAGTCGATTTTGCTCGAGATCAAGAGCTCGTTTTCCGACCCGCATGGGGTTCTCTCCGGCTGGATATCCAACAGCTTCGATCACCACTGCTCTTGGGTCGGGGTCTCCTGCAACGACAAATCCAGGGTCGTTTCGCTTAGTCTCGGCGGCAGCAGTGGCAAAGGAGGTAATTTTCGAGCTCTCTCTTGCTCTCAGAGTTTGAAATTTCCATTTCCGTTTGATGAGTTCGCTGTTTGGAGGAAAAGTTTAGTTATTAGTGGAAAATTGGGTGGAAAGGTTTCGCCTTGGGTGGGAAAGCTCACTGAGCTTAGAGTTTTATCGCTGCCATTTAATGAACTTGGTGGCGAAATTCCGAAGGAAATTTGGGGGTTGGAGAAGCTTGAAGTGCTTGATCTTGAGGGGAATTTGTTGAACGGAAGTTTGCCAACCCAGTTTTCGGGTTTAAGGATGTTGAGGGTTTTGAATCTTGGGTTTAATAGAATTGGTGGGGAGATTCCTATTTCGCTTTCAAGATGTGTGGATTTGGAGGTGTTGAATTTGGTAGGCAATGAAGTGAGTGGAACCATTCCTGGGTTTGTTGGTAGTTTTGCGAAGTTGCAGGGACTTTATTTGGCTCAAAATAGGTTAAATGGGTCTATACCAGCCACATTTGGAAGCTTTTGTCAAAATCTTGAACATCTTGATGTGTCGGGGAATTTCCTTGATGGGAAGATTCCTGGTAGTTTGGGGAATTGTCGGAGTTTACGGACTCTTTTGTTGTTTTCGAATATATTGGCTGGTAGCATTCCTCTAGAACTTGGTTGGATTCGAATGCTTGAAGTTCTAGATGTTTCAAGAAATAGCCTTAGCGGGCCAATCCCTGCTGAGCTTGGACAATGTGTCAATTTATCTGTTCTTGTCCTATCGAATCTTTTTAATCCATTGACAACCAATCAGAATACAAAAGGAGATACATCAATTGTTTTATCTAGTGGTTTGGACGATGATTACAATTATTACGAAGGCTCTTTTCCGGAGGAAATTACAACCCTTCCAAATTTGAAAATAGTTTGGGCTCCAAGGGCAACACTTGAGGGAAAGCTTCCGAGCAATTGGAGTGGTTGTGAGAACTTGGAAATGTTGAACTTAGCTCAGAACCTTTTCAGCGGAGAAGTCACTGGAGTATTTGAAAGATGCAAGAAGCTGCATTATCTAAATTTGGGATCAAATAAGCTGAGTGGGAAGATTGATGAGAAGTTTCCAGTTCCATGTATGACTGTTTTCAATGTCAGTGGAAACTTCATGTCTGGACCTGTGCCCGAGTTTCTGTACAGAGCATGCCCCCAGGTGCTCCCAAATTCAGATCGTGTCCGAGTCAACAATCTATCATTTCCATATCAAGTGCTCTTTACTTGCCAAACTCAGCTTGATAGTCATTTGCCTCTTTCCGGTGGTAGTTTCACTATGATTCATGATTTTAGTGGTAACAACTTCACTGGTCCAATCCAACACCTCCCTCTTGCAACTGAGAGATTGCAGAAGCAGACTGTTTATGCATTTCTTGCTGGTGGCAACAAGCTCACTGGATCATTTCCTGAAAGCTTACTAGGAAAATGTGACGGATTAGATGGAATGATTATTGATGTCAGCGACAATAAGTTGGCAGGTCGAATTCCTTTTGGGATTGGTGTGACGTGCAGATCTCTTGTCTTTCTTGACTTGAGCAGGAACCAGCTACAAGGTCAAATTCCCTCCGGTATCAGTCTGTTGAAGTATCTTAAGTATCTTGCCCTGGCTAACAACAACCTCACTGGTGCGATTCCTGCTAGCTTTGGGTGGTTGCACTCCCTTGAGGTGCTAAAACTGTCTTCAAATTCTCTTTCTGGTGATATTCCTCAAGGTCTTGTGAACTTGAAAAATTTAACTGTTTTTTTGCTCGACAACAATAAACTCACTGGGCACATCCCTTCAGGCTTGACTAATCTGAAATCCCTATCTACATTCAATGCCTCCTTCAATAACCTGTCTGGTTCATTTCCATTGAACAATAGTGCGATGAACTGCAGCAGTGTCCTTGGGAATCCTTTCCTTATTCCATGCCATGTTCCGCTAATTGCTCCATCTTCAGATCAGCTAGATAGGAATGGGAGTTCACAACATAACCCTGATTCCGCATCAGCAACTACTGGTGGTGATGATAACAGTGGATTAGGCTCAGTCGAGATCGCATCCATAGCATCTGCATCAGCTGTTGTTTTGGTTCTGCTCTCTCTCGTTATCCTTTTCTTTTATACGAGAAAATGGATACCAGATTCCAGGGTTCAGGGTTTTGAATATAAAGAAATGACTCTGTTTACAGACATTGGGGCTCCTTTGACATTTGAGATTATTGTTCAAGCAACAGGGAATTTTAATGGCAGCCACCACATTGGAAGTGGAGGATTTGGGGCCACTTACAAAGCCGAAATTGCTCCAGGAATCACTGTAGCTGTAAAAAGGCTAGCTGTTGGAAGGTTTCACGGTGTTCAACAGTTTCATGCTGAGATTAAAACCCTTGGCAGGGTGAGACACCCAAACCTGGTAACTTTAATAGGTTACCATGCCAGTGAAACTGAGATGTTGCTCATTTACAATTATTTACCAGGAGGGAATTTGGAAAAATTTATCAAGGAGAGATCTCAGAGGCCTTTTAATTGGCAAATTCTCCACAAGATTGCTTTGCATATAGCCCAAGCACTTGCTTATCTGCATGGCGAGTGTATTCCGCGTGTCCTTCACCGTGATGTCAAGCCAAGTAACATATTGTTGGATAATGAGTTCAATGCTTATTTGTCTGACTTTGGATTATCTAGGCTTTTGGGAACTTCAGAAACACACGTAACAACTGGTGTGGCAGGGACTTTTGGGTACGTAGCACCAGAGTATGCTATGACTTGCCATGTGTCTGAAAAGTCTGATGTTTACAGTTACGGTGTTATGCTGCTTGAATTGATGTCACATAAAGAAGCATTGGACCCTTCATTTTCGTCACATGGCCATGGTTTCAACATTGTGTCTTGGGTGGACATGCTGTTAGAACAGGGTCTGGATAAGGAGGTCTTCATAGAAGGGTTATGGGATGCAGGACCCCAGAATGATCTAGTCGAGATGTTGAATCTGGCTGTCTCGTGCACAGTCAAGAACCTCGCCTTTAGGCCAACAATGAAGCAAGTCGTCCAAAGATTAAAGCGAATCCAACCTATTTCAAGGAAGCCGGATAATGAGCAAAGTATAGAGCTATCGATCATAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1121

Amino Acids

122.03

Weight (kDa)

6.41

Isoelectric Point (pI)

32.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 68	1.4e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	127 - 230	3.1e-07	Leucine-rich repeat region
LRR_8	PF13855	228 - 286	5.8e-07	Leucine rich repeat
LRR_14	PF23598	590 - 691	2.4e-09	Leucine-rich repeat region
LRR_8	PF13855	591 - 650	1e-06	Leucine rich repeat
LRR_8	PF13855	615 - 674	5.7e-09	Leucine rich repeat
Pkinase	PF00069	830 - 1096	4.3e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	831 - 1098	1.1e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69270
fragaria_vesca	FvH4_4g29970
malus_domestica	MD13G1062000.v1.1 MD16G1060500.v1.1
prunus_persica	Prupe.1G287800_v2.0.a1
pyrus_communis	pycom13g05450 pycom16g05270
rosa_chinensis	RchiOBHm_Chr4g0438241
rosa_laevigata	RLG00000006378
rosa_multiflora	Rmu_sc0002682.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379330
rosa_rugosa	Rorug04G0308300
rosa_samantha	Rh4AG359300 Rh4BG370100 Rh4CG384300 Rh4DG365200
rosa_wichuraiana	Rw0G006390 Rw0G016330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1724
AccII	CGCG	2 cut(s)	29, 2836
AccIII	TCCGGA	1 cut(s)	1102
AciI	CCGC	8 cut(s)	27, 131, 237, 939, 1236, 2177, 2241, 2834
AclWI	GGATC	2 cut(s)	1300, 1663
AcoI	YGGCCR	1 cut(s)	3103
AcuI	CTGAAG	3 cut(s)	2022, 2178, 2919
AfaI	GTAC	2 cut(s)	1400, 2978
AfiI	CCNNNNNNNGG	8 cut(s)	136, 380, 526, 660, 1517, 2628, 2629, 2647
AflII	CTTAAG	1 cut(s)	1847
AflIII	ACRYGT	1 cut(s)	2944
AhdI	GACNNNNNGTC	1 cut(s)	968
AjiI	CACGTC	1 cut(s)	1764
AjnI	CCWGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
AjuI	GAANNNNNNNTTGG	2 cut(s)	508, 540
AloI	GAACNNNNNNTCC	4 cut(s)	2203, 2235, 2870, 2902
Alw21I	GWGCWC	5 cut(s)	119, 271, 1422, 1476, 3242
Alw26I	GTCTC	4 cut(s)	199, 236, 2629, 3238
Alw44I	GTGCAC	1 cut(s)	3238
AlwI	GGATC	2 cut(s)	1300, 1663
AlwNI	CAGNNNCTG	1 cut(s)	3154
Ama87I	CYCGRG	2 cut(s)	104, 1388
Aor13HI	TCCGGA	1 cut(s)	1102
AoxI	GGCC	5 cut(s)	941, 2517, 2753, 3103, 3263
ApaLI	GTGCAC	1 cut(s)	3238
ApeKI	GCWGC	6 cut(s)	240, 412, 1273, 2133, 2487, 3049
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
Asp700I	GAANNNNTTC	3 cut(s)	367, 1229, 2153
AspLEI	GCGC	2 cut(s)	31, 79
AspS9I	GGNCC	6 cut(s)	941, 1379, 1568, 2517, 3082, 3194
AsuHPI	GGTGA	5 cut(s)	164, 1967, 2273, 2644, 2837
AsuII	TTCGAA	2 cut(s)	854, 898
AsuNHI	GCTAGC	2 cut(s)	1895, 2569
AvaI	CYCGRG	2 cut(s)	104, 1388
AvaII	GGWCC	4 cut(s)	1379, 1568, 3082, 3194
BaeGI	GKGCMC	3 cut(s)	1389, 2031, 3242
BalI	TGGCCA	1 cut(s)	3105
BanII	GRGCYC	4 cut(s)	119, 271, 1147, 2440
BauI	CACGAG	1 cut(s)	3235
BbsI	GAAGAC	2 cut(s)	1929, 3160
Bbv12I	GWGCWC	5 cut(s)	119, 271, 1422, 1476, 3242
BbvI	GCAGC	6 cut(s)	252, 399, 1260, 2145, 2499, 3036
BccI	CCATC	3 cut(s)	788, 1691, 2197
BciT130I	CCWGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
BciVI	GTATCC	1 cut(s)	2367
BcoDI	GTCTC	4 cut(s)	199, 236, 2629, 3238
BfmI	CTRYAG	2 cut(s)	2131, 2553
BfoI	RGCGCY	1 cut(s)	80
BfrI	CTTAAG	1 cut(s)	1847
BfuAI	ACCTGC	1 cut(s)	1724
BfuI	GTATCC	1 cut(s)	2367
BglII	AGATCT	2 cut(s)	1769, 2744
BisI	GCNGC	7 cut(s)	238, 241, 413, 1274, 2134, 2488, 3050
BlpI	GCTNAGC	1 cut(s)	954
BlsI	GCNGC	7 cut(s)	239, 242, 414, 1275, 2135, 2489, 3051
Bme1390I	CCNGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
Bme18I	GGWCC	4 cut(s)	1379, 1568, 3082, 3194
BmeRI	GACNNNNNGTC	1 cut(s)	968
BmeT110I	CYCGRG	2 cut(s)	104, 1388
BmgBI	CACGTC	1 cut(s)	1764
BmgT120I	GGNCC	6 cut(s)	941, 1379, 1568, 2517, 3082, 3194
BmiI	GGNNCC	7 cut(s)	652, 1146, 1799, 2439, 2518, 3084, 3196
BmrFI	CCNGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
BmrI	ACTGGG	2 cut(s)	514, 2034
BmsI	GCATC	6 cut(s)	1253, 2252, 2306, 2315, 2321, 3178
BmtI	GCTAGC	2 cut(s)	1899, 2573
BmuI	ACTGGG	2 cut(s)	514, 2034
BpiI	GAAGAC	2 cut(s)	1929, 3160
BpmI	CTGGAG	2 cut(s)	1269, 2526
Bpu10I	CCTNAGC	1 cut(s)	935
Bpu1102I	GCTNAGC	1 cut(s)	954
Bpu14I	TTCGAA	2 cut(s)	854, 898
BpuEI	CTTGAG	5 cut(s)	506, 1181, 1810, 1940, 1950
Bsa29I	ATCGAT	1 cut(s)	3350
BsaAI	YACGTR	2 cut(s)	2947, 2980
BsaI	GGTCTC	1 cut(s)	199
BsaWI	WCCGGW	3 cut(s)	1102, 1517, 1823
BsaXI	ACNNNNNCTCC	4 cut(s)	319, 349, 1177, 1207
Bsc4I	CCNNNNNNNGG	8 cut(s)	136, 380, 526, 660, 1517, 2628, 2629, 2647
Bse118I	RCCGGY	1 cut(s)	79
Bse3DI	GCAATG	1 cut(s)	643
BseAI	TCCGGA	1 cut(s)	1102
BseBI	CCWGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
BseCI	ATCGAT	1 cut(s)	3350
BseGI	GGATG	4 cut(s)	543, 2031, 2297, 3193
BseLI	CCNNNNNNNGG	8 cut(s)	136, 380, 526, 660, 1517, 2628, 2629, 2647
BseMI	GCAATG	1 cut(s)	643
BseSI	GKGCMC	3 cut(s)	1389, 2031, 3242
BseXI	GCAGC	6 cut(s)	252, 399, 1260, 2145, 2499, 3036
BsgI	GTGCAG	1 cut(s)	1786
Bsh1236I	CGCG	2 cut(s)	29, 2836
BshFI	GGCC	5 cut(s)	943, 2519, 2755, 3105, 3265
BshVI	ATCGAT	1 cut(s)	3350
BsiHKAI	GWGCWC	5 cut(s)	119, 271, 1422, 1476, 3242
BsiHKCI	CYCGRG	2 cut(s)	104, 1388
BsiSI	CCGG	6 cut(s)	80, 147, 1103, 1518, 1824, 3323
BslFI	GGGAC	2 cut(s)	705, 2980
BslI	CCNNNNNNNGG	8 cut(s)	136, 380, 526, 660, 1517, 2628, 2629, 2647
BsmAI	GTCTC	4 cut(s)	199, 236, 2629, 3238
BsmFI	GGGAC	2 cut(s)	705, 2980
BsmI	GAATGC	2 cut(s)	873, 906
BsnI	GGCC	5 cut(s)	943, 2519, 2755, 3105, 3265
Bso31I	GGTCTC	1 cut(s)	199
BsoBI	CYCGRG	2 cut(s)	104, 1388
Bsp119I	TTCGAA	2 cut(s)	854, 898
Bsp1286I	GDGCHC	9 cut(s)	119, 271, 1147, 1389, 1422, 1476, 2031, 2440, 3242
Bsp13I	TCCGGA	1 cut(s)	1102
Bsp1407I	TGTACA	1 cut(s)	1398
Bsp1720I	GCTNAGC	1 cut(s)	954
Bsp19I	CCATGG	1 cut(s)	3106
BspACI	CCGC	8 cut(s)	27, 131, 237, 939, 1236, 2177, 2241, 2834
BspANI	GGCC	5 cut(s)	943, 2519, 2755, 3105, 3265
BspDI	ATCGAT	1 cut(s)	3350
BspEI	TCCGGA	1 cut(s)	1102
BspFNI	CGCG	2 cut(s)	29, 2836
BspHI	TCATGA	1 cut(s)	1540
BspLI	GGNNCC	7 cut(s)	652, 1146, 1799, 2439, 2518, 3084, 3196
BspMAI	CTGCAG	1 cut(s)	2135
BspMI	ACCTGC	1 cut(s)	1724
BspOI	GCTAGC	2 cut(s)	1899, 2573
BspPI	GGATC	2 cut(s)	1300, 1663
BspT104I	TTCGAA	2 cut(s)	854, 898
BspTI	CTTAAG	1 cut(s)	1847
BspTNI	GGTCTC	1 cut(s)	199
BsrDI	GCAATG	1 cut(s)	643
BsrFI	RCCGGY	1 cut(s)	79
BsrGI	TGTACA	1 cut(s)	1398
BssAI	RCCGGY	1 cut(s)	79
BssSI	CACGAG	1 cut(s)	3235
BssT1I	CCWWGG	5 cut(s)	374, 1148, 2143, 2623, 3106
Bst2BI	CACGAG	1 cut(s)	3235
Bst2UI	CCWGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
BstAFI	CTTAAG	1 cut(s)	1847
BstAPI	GCANNNNNTGC	1 cut(s)	1631
BstAUI	TGTACA	1 cut(s)	1398
BstBAI	YACGTR	2 cut(s)	2947, 2980
BstBI	TTCGAA	2 cut(s)	854, 898
BstC8I	GCNNGC	4 cut(s)	941, 1408, 1897, 2571
BstDSI	CCRYGG	1 cut(s)	3106
BstEII	GGTNACC	1 cut(s)	2663
BstF5I	GGATG	4 cut(s)	543, 2031, 2297, 3193
BstFNI	CGCG	2 cut(s)	29, 2836
BstH2I	RGCGCY	1 cut(s)	80
BstHHI	GCGC	2 cut(s)	31, 79
BstMAI	GTCTC	4 cut(s)	199, 236, 2629, 3238
BstMWI	GCNNNNNNNGC	8 cut(s)	246, 394, 475, 1151, 1631, 1892, 2303, 2537
BstNI	CCWGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
BstNSI	RCATGY	2 cut(s)	1410, 3139
BstPI	GGTNACC	1 cut(s)	2663
BstSCI	CCNGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
BstSFI	CTRYAG	2 cut(s)	2131, 2553
BstSLI	GKGCMC	3 cut(s)	1389, 2031, 3242
BstSNI	TACGTA	1 cut(s)	2980
BstUI	CGCG	2 cut(s)	29, 2836
BstV1I	GCAGC	6 cut(s)	252, 399, 1260, 2145, 2499, 3036
BstV2I	GAAGAC	2 cut(s)	1929, 3160
BstX2I	RGATCY	2 cut(s)	1769, 2744
BstXI	CCANNNNNNTGG	1 cut(s)	2498
BstYI	RGATCY	2 cut(s)	1769, 2744
Bsu15I	ATCGAT	1 cut(s)	3350
BsuI	GTATCC	1 cut(s)	2367
BsuRI	GGCC	5 cut(s)	943, 2519, 2755, 3105, 3265
BsuTUI	ATCGAT	1 cut(s)	3350
BtgI	CCRYGG	1 cut(s)	3106
BtgZI	GCGATG	1 cut(s)	2138
BtrI	CACGTC	1 cut(s)	1764
BtsCI	GGATG	4 cut(s)	543, 2031, 2297, 3193
BtsI	GCAGTG	3 cut(s)	175, 250, 2143
BveI	ACCTGC	1 cut(s)	1724
Cac8I	GCNNGC	4 cut(s)	941, 1408, 1897, 2571
CaiI	CAGNNNCTG	1 cut(s)	3154
CciI	TCATGA	1 cut(s)	1540
CfoI	GCGC	2 cut(s)	31, 79
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	6 cut(s)	941, 1379, 1568, 2517, 3082, 3194
ClaI	ATCGAT	1 cut(s)	3350
CsiI	ACCWGGT	1 cut(s)	2646
Csp6I	GTAC	2 cut(s)	1399, 2977
CviQI	GTAC	2 cut(s)	1399, 2977
DriI	GACNNNNNGTC	1 cut(s)	968
EaeI	YGGCCR	1 cut(s)	3103
Eam1105I	GACNNNNNGTC	1 cut(s)	968
Ecl136II	GAGCTC	2 cut(s)	117, 269
Eco105I	TACGTA	1 cut(s)	2980
Eco130I	CCWWGG	5 cut(s)	374, 1148, 2143, 2623, 3106
Eco147I	AGGCCT	1 cut(s)	2755
Eco24I	GRGCYC	4 cut(s)	119, 271, 1147, 2440
Eco31I	GGTCTC	1 cut(s)	199
Eco32I	GATATC	1 cut(s)	156
Eco47I	GGWCC	4 cut(s)	1379, 1568, 3082, 3194
Eco53kI	GAGCTC	2 cut(s)	117, 269
Eco57I	CTGAAG	3 cut(s)	2022, 2178, 2919
Eco88I	CYCGRG	2 cut(s)	104, 1388
Eco91I	GGTNACC	1 cut(s)	2663
EcoICRI	GAGCTC	2 cut(s)	117, 269
EcoO109I	RGGNCCY	1 cut(s)	3194
EcoO65I	GGTNACC	1 cut(s)	2663
EcoRI	GAATTC	1 cut(s)	1740
EcoRII	CCWGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
EcoRV	GATATC	1 cut(s)	156
EcoT14I	CCWWGG	5 cut(s)	374, 1148, 2143, 2623, 3106
EcoT22I	ATGCAT	1 cut(s)	1627
EcoT38I	GRGCYC	4 cut(s)	119, 271, 1147, 2440
ErhI	CCWWGG	5 cut(s)	374, 1148, 2143, 2623, 3106
FalI	AAGNNNNNCTT	2 cut(s)	3152, 3184
FaqI	GGGAC	2 cut(s)	705, 2980
FauI	CCCGC	2 cut(s)	138, 932
FauNDI	CATATG	1 cut(s)	12
Fnu4HI	GCNGC	7 cut(s)	238, 241, 413, 1274, 2134, 2488, 3050
FokI	GGATG	4 cut(s)	550, 2018, 2284, 3200
FriOI	GRGCYC	4 cut(s)	119, 271, 1147, 2440
Fsp4HI	GCNGC	7 cut(s)	238, 241, 413, 1274, 2134, 2488, 3050
GlaI	GCGC	2 cut(s)	30, 78
GluI	GCNGC	7 cut(s)	238, 241, 413, 1274, 2134, 2488, 3050
GsuI	CTGGAG	2 cut(s)	1269, 2526
HaeII	RGCGCY	1 cut(s)	80
HaeIII	GGCC	5 cut(s)	943, 2519, 2755, 3105, 3265
HapII	CCGG	6 cut(s)	80, 147, 1103, 1518, 1824, 3323
HhaI	GCGC	2 cut(s)	31, 79
Hin6I	GCGC	2 cut(s)	29, 77
HinP1I	GCGC	2 cut(s)	29, 77
HincII	GTYRAC	1 cut(s)	1447
HindII	GTYRAC	1 cut(s)	1447
HindIII	AAGCTT	4 cut(s)	467, 745, 1169, 1669
HpaII	CCGG	6 cut(s)	80, 147, 1103, 1518, 1824, 3323
HphI	GGTGA	5 cut(s)	164, 1967, 2273, 2644, 2837
Hpy166II	GTNNAC	8 cut(s)	833, 1447, 1978, 2222, 2424, 3031, 3133, 3240
Hpy8I	GTNNAC	8 cut(s)	833, 1447, 1978, 2222, 2424, 3031, 3133, 3240
HpyCH4IV	ACGT	3 cut(s)	1763, 2946, 2979
HpyF10VI	GCNNNNNNNGC	8 cut(s)	246, 394, 475, 1151, 1631, 1892, 2303, 2537
HpySE526I	ACGT	3 cut(s)	1763, 2946, 2979
HspAI	GCGC	2 cut(s)	29, 77
Kpn2I	TCCGGA	1 cut(s)	1102
LmnI	GCTCC	5 cut(s)	1150, 1425, 2191, 2443, 2545
Lsp1109I	GCAGC	6 cut(s)	252, 399, 1260, 2145, 2499, 3036
LweI	GCATC	6 cut(s)	1253, 2252, 2306, 2315, 2321, 3178
MabI	ACCWGGT	1 cut(s)	2646
MaeII	ACGT	3 cut(s)	1763, 2946, 2979
MboII	GAAGA	5 cut(s)	811, 1324, 1929, 2184, 3160
MfeI	CAATTG	2 cut(s)	1047, 1180
MflI	RGATCY	2 cut(s)	1769, 2744
MhlI	GDGCHC	9 cut(s)	119, 271, 1147, 1389, 1422, 1476, 2031, 2440, 3242
MlsI	TGGCCA	1 cut(s)	3105
MluNI	TGGCCA	1 cut(s)	3105
MlyI	GAGTC	3 cut(s)	832, 1452, 2410
MmeI	TCCRAC	9 cut(s)	150, 183, 441, 806, 872, 1600, 2557, 2856, 3328
Mox20I	TGGCCA	1 cut(s)	3105
Mph1103I	ATGCAT	1 cut(s)	1627
MroI	TCCGGA	1 cut(s)	1102
MroXI	GAANNNNTTC	3 cut(s)	367, 1229, 2153
MscI	TGGCCA	1 cut(s)	3105
MslI	CAYNNNNRTG	4 cut(s)	771, 2673, 2850, 3105
Msp20I	TGGCCA	1 cut(s)	3105
MspA1I	CMGCKG	2 cut(s)	1236, 2318
MspCI	CTTAAG	1 cut(s)	1847
MspI	CCGG	6 cut(s)	80, 147, 1103, 1518, 1824, 3323
MspR9I	CCNGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
MunI	CAATTG	2 cut(s)	1047, 1180
Mva1269I	GAATGC	2 cut(s)	873, 906
MvaI	CCWGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
MvnI	CGCG	2 cut(s)	29, 2836
MwoI	GCNNNNNNNGC	8 cut(s)	246, 394, 475, 1151, 1631, 1892, 2303, 2537
NcoI	CCATGG	1 cut(s)	3106
NdeI	CATATG	1 cut(s)	12
NheI	GCTAGC	2 cut(s)	1895, 2569
NlaIV	GGNNCC	7 cut(s)	652, 1146, 1799, 2439, 2518, 3084, 3196
NmeAIII	GCCGAG	1 cut(s)	213
NmuCI	GTSAC	5 cut(s)	1243, 1685, 1759, 3063, 3096
NsiI	ATGCAT	1 cut(s)	1627
NspI	RCATGY	2 cut(s)	1410, 3139
NspV	TTCGAA	2 cut(s)	854, 898
PaeI	GCATGC	1 cut(s)	1410
PaeR7I	CTCGAG	1 cut(s)	104
PagI	TCATGA	1 cut(s)	1540
PceI	AGGCCT	1 cut(s)	2755
PctI	GAATGC	2 cut(s)	873, 906
PdmI	GAANNNNTTC	3 cut(s)	367, 1229, 2153
PfoI	TCCNGGA	1 cut(s)	2542
PkrI	GCNGC	7 cut(s)	239, 242, 414, 1275, 2135, 2489, 3051
PleI	GAGTC	3 cut(s)	832, 1451, 2410
PpsI	GAGTC	3 cut(s)	832, 1451, 2410
Ppu21I	YACGTR	2 cut(s)	2947, 2980
PpuMI	RGGWCCY	1 cut(s)	3194
Psp124BI	GAGCTC	2 cut(s)	119, 271
Psp5II	RGGWCCY	1 cut(s)	3194
Psp6I	CCWGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
PspEI	GGTNACC	1 cut(s)	2663
PspGI	CCWGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
PspN4I	GGNNCC	7 cut(s)	652, 1146, 1799, 2439, 2518, 3084, 3196
PspPI	GGNCC	6 cut(s)	941, 1379, 1568, 2517, 3082, 3194
PspPPI	RGGWCCY	1 cut(s)	3194
PstI	CTGCAG	1 cut(s)	2135
PstNI	CAGNNNCTG	1 cut(s)	3154
PsuI	RGATCY	2 cut(s)	1769, 2744
PvuII	CAGCTG	1 cut(s)	2318
RsaI	GTAC	2 cut(s)	1400, 2978
RsaNI	GTAC	2 cut(s)	1399, 2977
RseI	CAYNNNNRTG	4 cut(s)	771, 2673, 2850, 3105
SacI	GAGCTC	2 cut(s)	119, 271
SatI	GCNGC	7 cut(s)	238, 241, 413, 1274, 2134, 2488, 3050
Sau96I	GGNCC	6 cut(s)	941, 1379, 1568, 2517, 3082, 3194
SchI	GAGTC	3 cut(s)	832, 1452, 2410
ScrFI	CCNGG	9 cut(s)	214, 660, 807, 1415, 1862, 2386, 2544, 2648, 2712
SduI	GDGCHC	9 cut(s)	119, 271, 1147, 1389, 1422, 1476, 2031, 2440, 3242
SexAI	ACCWGGT	1 cut(s)	2646
SfaNI	GCATC	6 cut(s)	1253, 2252, 2306, 2315, 2321, 3178
SfcI	CTRYAG	2 cut(s)	2131, 2553
Sfr274I	CTCGAG	1 cut(s)	104
SfuI	TTCGAA	2 cut(s)	854, 898
SinI	GGWCC	4 cut(s)	1379, 1568, 3082, 3194
SlaI	CTCGAG	1 cut(s)	104
SmiMI	CAYNNNNRTG	4 cut(s)	771, 2673, 2850, 3105
SmlI	CTYRAG	7 cut(s)	104, 485, 1160, 1789, 1847, 1919, 1965
SmoI	CTYRAG	7 cut(s)	104, 485, 1160, 1789, 1847, 1919, 1965
SnaBI	TACGTA	1 cut(s)	2980
SphI	GCATGC	1 cut(s)	1410
SseBI	AGGCCT	1 cut(s)	2755
SsiI	CCGC	8 cut(s)	27, 131, 237, 939, 1236, 2177, 2241, 2834
SstI	GAGCTC	2 cut(s)	119, 271
StuI	AGGCCT	1 cut(s)	2755
StyD4I	CCNGG	9 cut(s)	212, 658, 805, 1413, 1860, 2384, 2542, 2646, 2710
StyI	CCWWGG	5 cut(s)	374, 1148, 2143, 2623, 3106
TaiI	ACGT	3 cut(s)	1766, 2949, 2982
TatI	WGTACW	1 cut(s)	1398
TauI	GCSGC	1 cut(s)	240
TseFI	GTSAC	5 cut(s)	1243, 1685, 1759, 3063, 3096
TseI	GCWGC	6 cut(s)	240, 412, 1273, 2133, 2487, 3049
Tsp45I	GTSAC	5 cut(s)	1243, 1685, 1759, 3063, 3096
TspGWI	ACGGA	4 cut(s)	294, 519, 850, 1704
Vha464I	CTTAAG	1 cut(s)	1847
VneI	GTGCAC	1 cut(s)	3238
VpaK11BI	GGWCC	4 cut(s)	1379, 1568, 3082, 3194
XbaI	TCTAGA	2 cut(s)	880, 913
XceI	RCATGY	2 cut(s)	1410, 3139
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2501
XhoI	CTCGAG	1 cut(s)	104
XmnI	GAANNNNTTC	3 cut(s)	367, 1229, 2153
Zsp2I	ATGCAT	1 cut(s)	1627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.