Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00245

Orthogroup ID

OG0012825

Physical Location & Seq

Genomic Coordinates Reverse (-)

150985 .. 154379

Gene Structure 3395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G006390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGAGATGGCGTGGCCTCAGAAGCACCCTGTTTTTCCTCAAAGTCTTCTCCTTTTTGCTGTGCTTTTCAGTTTCCGGCGACAAAAACCGCTGTTCGGAGAAGTCGGTTCTGCTCGAGCTGAAGACCTCGGTGTCGGACCCAGATGGGGTTCTGTCGACGTGGGCCCACGGCAGCTCTGATCACCACTGTTCCTGGTTCGGGGTCTCCTGCGACTCGAAATCCAAGGTTGTCTCGCTCAGCGTTAGTGGTGGGAAATTAGGTGGAAAGCTTTCACCTTTGGTTGGGAAACTTACTGAGCTTAGAGTTTTATCGCTGCCGTTTAATGAACTTAGTGGTGAGATTCCCGAGGAGGTTTGGGGGTTGGAGAAACTTGAAGTGCTTGATCTTGAGGGTAATTTGTTGGAGGGGAAGTTGCCGAGTTGGTTCTCGGGTTTGAGAAAGTTGAGGGTTTTCAATGTTGGGTTTAATAGGATTGGTGGGGAGGTTCCGGTTTCGCTTTCGAAATTTGCAGGGGCTTTATTTGGCTCAAATAGGTTAAATGGATCTATTCCGGCCAAATTTGGAAGGTCTTGTTTGAATCTTGAGCATTTTGATGTGTCGGGGAATTTCCTTGTTGGGAATATTCCGCCTACTTTGGGGAGTTGTCTGCGGTTACGGACGCTATTGTTGTTTTCAAATACTCTGGATGGTGGCATTCCTCGTGAACTTGGTAAGATTCGGGGGCTTGAAGTTCTGGATGTGTCTAGAAATAGCCTTAGCGGTCCAATCCCCGCTGAGCTCGGACAGTGTGTCAATTTGTCGGTACTTGTCCTTTCGAGTCTTTTTAATCCGTTGCCAATCAATCAGAATGCAAATGGAGATTCATCGGTTAACTCATATAAAGGAGTGGATGATGATTACAATTTTTATGAAGGCTCCATTCCCCAGGAGATTACAGGCTTACCAAATTTGAGAATAGTTTGGGCGCCAAGGGCAACCCTCGAAGGCCAGTTTCCGAGCAATTGGGGTGGTTGTGAGAACTTGGAAATGGTGAACTTGGCTCAGAACCTTTTCACTGGAGAAGTGATTGGAGTGTTTGATAGATGCAAGAAGCTGCAGTATCTGAATTTGAGCTCAAATAAGCTGAGTGGGAAGCTTGATGACAAACTCCCAGTTCCTTGTATGACCGTTTTCAGTGTCAGTGGGAACCTCTTGTCTGGACCCATCCCCACATTTAATTTCGCAGTGTGCCCCCACGTGCCCCGAAATTCAGATCTTGTCCGAGTCCAGAATCCATCATTTGCATATCAAGTTCTCTTTACTTGCAGAACTTATCTTGACACCCATTTACCACTTTTTGGTGCTAGTTTCACATTGATTCACGATTTTAGTGGTAACAACTTCACTGGTCCAATCCAACACCTTCCTCTTGCACCTGAGAGGTTGGGGAAGCGAACTGTTTATGCATTTCTTGCTGGTGGGAACAAGTTCACTGGATCATTTACTGAAACCTTAAAAGGAAAGTGTGATGGCTTACATGGAATGATATTTAATGTTAGCAACAACAAGTTATCAGGCCACATTCCTTTCAAGATTGGCAAGATGTGCTGGTCTCTCAGACTCTTGGATGCCTCTGGAAATCTCTTATCTGGTTCAATACCTCCAGATCTTGGGGATGTGAAGGCTCTTGTTTTTCTTGACTTGAGCAGGAACCAGTTGCAGGGTCAAATTCCCGCAGGTATCAGTAATTTGAAGTATCTCAAGTATCTATCCTTGGCTAATAACAACCTTACTGGTACGATTCCTGCTAGCTTTGTGGAGTTACGCTCCCTTGAAGTGTTAAAGCTATCTTCAAACTCTCTTTCTGGTGATATCCCGCAAGGTCTTGTTAAGTTGAAAAACCTAACTATTTTTAGGCTTGACAACAATAAGCTCTCTGGCCGCATCCCTTCTGGTTTGACTAATGTGAGATCCCTGTCTACATTTAATGTCTCATATAATAACTTGTCTGGTTCATTTCCATTGAACAATAATATGATGAACTGTAGCGGTGTCCTTGGAAACCCTTTCGTTAATCAATGCCGCATTGTTTCCCTTGCTGCGCCATACTCAGCTCAGCCGGTTAGCTCTGCCAATTCACCCCCTGATTATCAGTCAGCAAGTGCTCCTGGAAAGGATGGAATAAACTCAATTGAGATTGCATCCATAGTATCTGCATCAGCTATCGTGTTGGTTCTCTTCACTCTTGTTATCCTCTTCTTTTACACAAGAAAATGGATTCCAGATTCCAGAGTTCAGGGTTTTGAAAATAAAGAAATTACAATTTTCACGGAAATTGGGGCACCATTGACGTTTGAGAATATTGTTCAAGCAACAGGGAATTTTAATGCCAGTAACTACATTGGGAGTGGAGGATTTGGGGCAACATACAAAGCAGAAATTGCTCCAGGAACCATAATAGCTGTGAAGAGGCTTGCCGTTGGAAGGTTTCACGGTGTTCAACAGTTCCATGCTGAGATTAAGACCCTCGGAAGGGTGAGACACCCCAACCTGGTAACTTTAATAGGTTACCATGCCAGTGAAACCGAGATGCTACTCATATATAATTATTTACCAGGAGGGAATTTGGAAAATTTTATTCAGGAAAGATCTAGAAGATCTTTTACTTGGAAGATTCTCCACAAGATTGCTCTGGATATTGCCCATGCACTTTCTTATCTGCATGACGAGTGTATCCCGCGTGTCCTACACCGTGATGTCAAGCCAAGCAATATACTGTTAGATGATAACTTGAATGCTTATTTGTCTGACTTTGGATTGTCTAGGCTGCTGGGAACTTCTGAAACGCATGCTACAACTGGTGTTGCAGGAACTTTTGGGTACGTAGCACCCGAGTATGCTATGACCTGTCGTGTGTCTGAGAAGGCTGATGTTTACAGTTACGGTGTTGTGCTGCTTGAATTGATTTCTGATAAGAAAGCCCTGGACCCATCATTTTCTTCACATGGAAATGGTTTCAACATTGTTTCTTGGGCGTGCATGCTATTAAGAATGGGTCGGGCTAAGGACGTATTCATGGAAGGACTATGGGAGGCAGGACCCGAAGATGATCTGGTTGAGATGCTGTACTTGGCTGTCAAATGTACTGTTGAGACCCTCTCCATTAGGCCTACAATGAAGCAAGTTGTCCAAATACTGAAGCGAATACAACCTGCTTCAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0001101 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009698 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009808 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009908 GO:0009942 GO:0009945 GO:0009956 GO:0009987 GO:0010035 GO:0010073 GO:0010152 GO:0010154 GO:0016020 GO:0016043 GO:0019748 GO:0021700 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042221 GO:0043933 GO:0044085 GO:0044237 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048507 GO:0048508 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051260 GO:0051704 GO:0061458 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0099402 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1067

Amino Acids

116.5

Weight (kDa)

8.63

Isoelectric Point (pI)

32.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 71	7.8e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	94 - 154	6.4e-06	Leucine-rich repeat region
LRR_8	PF13855	99 - 158	3.3e-07	Leucine rich repeat
LRR_14	PF23598	548 - 656	7.9e-11	Leucine-rich repeat region
LRR_8	PF13855	575 - 614	2.1e-07	Leucine rich repeat
Pkinase	PF00069	790 - 991	5.1e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	791 - 1058	1.2e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69270
fragaria_vesca	FvH4_4g29970
malus_domestica	MD13G1062000.v1.1 MD16G1060500.v1.1
prunus_persica	Prupe.1G287800_v2.0.a1
pyrus_communis	pycom13g05450 pycom16g05270
rosa_chinensis	RchiOBHm_Chr4g0438241
rosa_laevigata	RLG00000006378
rosa_multiflora	Rmu_sc0002682.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379330
rosa_rugosa	Rorug04G0308300
rosa_samantha	Rh4AG359300 Rh4BG370100 Rh4CG384300 Rh4DG365200
rosa_wichuraiana	Rw0G006390 Rw0G016330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	130
Acc36I	ACCTGC	2 cut(s)	1706, 3199
AccB1I	GGYRCC	2 cut(s)	964, 2320
AccB7I	CCANNNNNTGG	2 cut(s)	1337, 1649
AccI	GTMKAC	2 cut(s)	155, 1958
AccII	CGCG	1 cut(s)	2719
AclWI	GGATC	3 cut(s)	550, 1483, 1944
AcoI	YGGCCR	2 cut(s)	552, 1918
AcuI	CTGAAG	2 cut(s)	140, 3197
AcvI	CACGTG	1 cut(s)	1237
AcyI	GRCGYC	1 cut(s)	965
AfaI	GTAC	5 cut(s)	804, 1777, 2861, 3107, 3124
AhdI	GACNNNNNGTC	1 cut(s)	788
AjiI	CACGTC	1 cut(s)	159
AjnI	CCWGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
Alw21I	GWGCWC	3 cut(s)	780, 1115, 2146
Alw26I	GTCTC	6 cut(s)	208, 235, 1596, 1975, 2512, 3125
AlwI	GGATC	3 cut(s)	550, 1483, 1944
AlwNI	CAGNNNCTG	1 cut(s)	1102
Ama87I	CYCGRG	4 cut(s)	113, 344, 427, 2870
AoxI	GGCC	7 cut(s)	13, 162, 552, 985, 1555, 1918, 3146
ApaI	GGGCCC	1 cut(s)	166
ApeKI	GCWGC	6 cut(s)	171, 313, 1093, 2078, 2806, 2932
Asp700I	GAANNNNTTC	3 cut(s)	268, 1049, 1466
AspLEI	GCGC	2 cut(s)	967, 2083
AspS9I	GGNCC	8 cut(s)	136, 162, 163, 761, 1199, 1388, 2965, 3077
AsuHPI	GGTGA	7 cut(s)	173, 264, 347, 1042, 1859, 2109, 2527
AsuII	TTCGAA	1 cut(s)	500
AsuNHI	GCTAGC	1 cut(s)	1787
AvaI	CYCGRG	4 cut(s)	113, 344, 427, 2870
AvaII	GGWCC	6 cut(s)	136, 761, 1199, 1388, 2965, 3077
BaeGI	GKGCMC	4 cut(s)	166, 1232, 1242, 2323
BaeI	ACNNNNGTAYC	2 cut(s)	2695, 2728
BanI	GGYRCC	2 cut(s)	964, 2320
BanII	GRGCYC	3 cut(s)	166, 780, 1115
BarI	GAAGNNNNNNTAC	2 cut(s)	2626, 2658
BauI	CACGAG	1 cut(s)	699
BbrPI	CACGTG	1 cut(s)	1237
BbsI	GAAGAC	2 cut(s)	37, 128
Bbv12I	GWGCWC	3 cut(s)	780, 1115, 2146
BbvI	GCAGC	6 cut(s)	183, 300, 1080, 2065, 2793, 2919
BccI	CCATC	7 cut(s)	137, 680, 1211, 1282, 1502, 2150, 2977
BceAI	ACGGC	3 cut(s)	184, 301, 2441
BciT130I	CCWGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
BciVI	GTATCC	1 cut(s)	2723
BclI	TGATCA	1 cut(s)	178
BcoDI	GTCTC	6 cut(s)	208, 235, 1596, 1975, 2512, 3125
BfaI	CTAG	6 cut(s)	744, 1344, 1788, 2631, 2802, 3202
BfmI	CTRYAG	2 cut(s)	1094, 2023
BfoI	RGCGCY	1 cut(s)	968
BfuAI	ACCTGC	2 cut(s)	1706, 3199
BfuI	GTATCC	1 cut(s)	2723
BglII	AGATCT	4 cut(s)	1252, 1645, 2627, 2636
BisI	GCNGC	8 cut(s)	172, 314, 1094, 1921, 2062, 2079, 2807, 2933
BlpI	GCTNAGC	3 cut(s)	236, 774, 2094
BlsI	GCNGC	8 cut(s)	173, 315, 1095, 1922, 2063, 2080, 2808, 2934
Bme1390I	CCNGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
Bme18I	GGWCC	6 cut(s)	136, 761, 1199, 1388, 2965, 3077
BmeRI	GACNNNNNGTC	1 cut(s)	788
BmeT110I	CYCGRG	4 cut(s)	113, 344, 427, 2870
BmgBI	CACGTC	1 cut(s)	159
BmgT120I	GGNCC	8 cut(s)	136, 162, 163, 761, 1199, 1388, 2965, 3077
BmrFI	CCNGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
BmrI	ACTGGG	1 cut(s)	1145
BmsI	GCATC	7 cut(s)	1073, 1597, 1932, 2189, 2204, 2559, 3090
BmtI	GCTAGC	1 cut(s)	1791
BmuI	ACTGGG	1 cut(s)	1145
BpiI	GAAGAC	2 cut(s)	37, 128
BplI	GAGNNNNNCTC	4 cut(s)	1790, 1822, 3122, 3154
BpmI	CTGGAG	3 cut(s)	1077, 1626, 2409
Bpu10I	CCTNAGC	2 cut(s)	755, 3042
Bpu1102I	GCTNAGC	3 cut(s)	236, 774, 2094
Bpu14I	TTCGAA	1 cut(s)	500
BpuEI	CTTGAG	4 cut(s)	407, 602, 1702, 1724
BsaAI	YACGTR	2 cut(s)	1237, 2863
BsaHI	GRCGYC	1 cut(s)	965
BsaI	GGTCTC	3 cut(s)	208, 1596, 3125
BsaWI	WCCGGW	1 cut(s)	487
BsaXI	ACNNNNNCTCC	2 cut(s)	395, 425
Bse118I	RCCGGY	1 cut(s)	2098
BseBI	CCWGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
BseGI	GGATG	9 cut(s)	691, 742, 895, 1203, 1612, 1660, 1923, 2161, 2180
BseRI	GAGGAG	1 cut(s)	362
BseSI	GKGCMC	4 cut(s)	166, 1232, 1242, 2323
BseXI	GCAGC	6 cut(s)	183, 300, 1080, 2065, 2793, 2919
BseYI	CCCAGC	1 cut(s)	2809
Bsh1236I	CGCG	1 cut(s)	2719
BshFI	GGCC	7 cut(s)	15, 164, 554, 987, 1557, 1920, 3148
BshNI	GGYRCC	2 cut(s)	964, 2320
BsiHKAI	GWGCWC	3 cut(s)	780, 1115, 2146
BsiHKCI	CYCGRG	4 cut(s)	113, 344, 427, 2870
BsiSI	CCGG	4 cut(s)	75, 488, 551, 2099
BsmAI	GTCTC	6 cut(s)	208, 235, 1596, 1975, 2512, 3125
BsmI	GAATGC	3 cut(s)	693, 853, 2779
BsnI	GGCC	7 cut(s)	15, 164, 554, 987, 1557, 1920, 3148
Bso31I	GGTCTC	3 cut(s)	208, 1596, 3125
BsoBI	CYCGRG	4 cut(s)	113, 344, 427, 2870
Bsp119I	TTCGAA	1 cut(s)	500
Bsp120I	GGGCCC	1 cut(s)	162
Bsp1286I	GDGCHC	7 cut(s)	166, 780, 1115, 1232, 1242, 2146, 2323
Bsp1720I	GCTNAGC	3 cut(s)	236, 774, 2094
BspANI	GGCC	7 cut(s)	15, 164, 554, 987, 1557, 1920, 3148
BspFNI	CGCG	1 cut(s)	2719
BspMAI	CTGCAG	1 cut(s)	1098
BspMI	ACCTGC	2 cut(s)	1706, 3199
BspOI	GCTAGC	1 cut(s)	1791
BspPI	GGATC	3 cut(s)	550, 1483, 1944
BspT104I	TTCGAA	1 cut(s)	500
BspT107I	GGYRCC	2 cut(s)	964, 2320
BspTNI	GGTCTC	3 cut(s)	208, 1596, 3125
BsrFI	RCCGGY	1 cut(s)	2098
BssAI	RCCGGY	1 cut(s)	2098
BssNI	GRCGYC	1 cut(s)	965
BssSI	CACGAG	1 cut(s)	699
BssT1I	CCWWGG	4 cut(s)	222, 968, 1752, 2035
Bst2BI	CACGAG	1 cut(s)	699
Bst2UI	CCWGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
Bst6I	CTCTTC	3 cut(s)	2222, 2240, 2441
BstACI	GRCGYC	1 cut(s)	965
BstAPI	GCANNNNNTGC	3 cut(s)	1451, 1584, 2420
BstBAI	YACGTR	2 cut(s)	1237, 2863
BstBI	TTCGAA	1 cut(s)	500
BstC8I	GCNNGC	5 cut(s)	1789, 2454, 2829, 3016, 3020
BstDSI	CCRYGG	1 cut(s)	166
BstEII	GGTNACC	1 cut(s)	2546
BstF5I	GGATG	9 cut(s)	691, 742, 895, 1203, 1612, 1660, 1923, 2161, 2180
BstFNI	CGCG	1 cut(s)	2719
BstH2I	RGCGCY	1 cut(s)	968
BstHHI	GCGC	2 cut(s)	967, 2083
BstMAI	GTCTC	6 cut(s)	208, 235, 1596, 1975, 2512, 3125
BstMWI	GCNNNNNNNGC	5 cut(s)	21, 971, 1451, 1584, 2420
BstNI	CCWGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
BstNSI	RCATGY	2 cut(s)	2831, 3022
BstPI	GGTNACC	1 cut(s)	2546
BstSCI	CCNGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
BstSFI	CTRYAG	2 cut(s)	1094, 2023
BstSLI	GKGCMC	4 cut(s)	166, 1232, 1242, 2323
BstSNI	TACGTA	1 cut(s)	2863
BstUI	CGCG	1 cut(s)	2719
BstV1I	GCAGC	6 cut(s)	183, 300, 1080, 2065, 2793, 2919
BstV2I	GAAGAC	2 cut(s)	37, 128
BstX2I	RGATCY	6 cut(s)	542, 1252, 1645, 1949, 2627, 2636
BstYI	RGATCY	6 cut(s)	542, 1252, 1645, 1949, 2627, 2636
BsuI	GTATCC	1 cut(s)	2723
BsuRI	GGCC	7 cut(s)	15, 164, 554, 987, 1557, 1920, 3148
BtgI	CCRYGG	1 cut(s)	166
BtrI	CACGTC	1 cut(s)	159
BtsCI	GGATG	9 cut(s)	691, 742, 895, 1203, 1612, 1660, 1923, 2161, 2180
BtsI	GCAGTG	1 cut(s)	1230
BtsIMutI	CAGTG	9 cut(s)	184, 791, 1053, 1180, 1186, 1230, 1383, 1470, 2563
BveI	ACCTGC	2 cut(s)	1706, 3199
Cac8I	GCNNGC	5 cut(s)	1789, 2454, 2829, 3016, 3020
CaiI	CAGNNNCTG	1 cut(s)	1102
CfoI	GCGC	2 cut(s)	967, 2083
Cfr10I	RCCGGY	1 cut(s)	2098
Cfr13I	GGNCC	8 cut(s)	136, 162, 163, 761, 1199, 1388, 2965, 3077
CseI	GACGC	1 cut(s)	667
CsiI	ACCWGGT	1 cut(s)	2529
Csp6I	GTAC	5 cut(s)	803, 1776, 2860, 3106, 3123
CspCI	CAANNNNNGTGG	2 cut(s)	988, 1023
CviAII	CATG	9 cut(s)	1517, 2489, 2552, 2684, 2702, 2828, 2984, 3019, 3055
CviQI	GTAC	5 cut(s)	803, 1776, 2860, 3106, 3123
DinI	GGCGCC	1 cut(s)	966
DrdI	GACNNNNNNGTC	1 cut(s)	130
DriI	GACNNNNNGTC	1 cut(s)	788
DseDI	GACNNNNNNGTC	1 cut(s)	130
EaeI	YGGCCR	2 cut(s)	552, 1918
Eam1104I	CTCTTC	3 cut(s)	2222, 2240, 2441
Eam1105I	GACNNNNNGTC	1 cut(s)	788
EarI	CTCTTC	3 cut(s)	2222, 2240, 2441
EciI	GGCGGA	1 cut(s)	615
Ecl136II	GAGCTC	2 cut(s)	778, 1113
Eco105I	TACGTA	1 cut(s)	2863
Eco130I	CCWWGG	4 cut(s)	222, 968, 1752, 2035
Eco147I	AGGCCT	1 cut(s)	3148
Eco24I	GRGCYC	3 cut(s)	166, 780, 1115
Eco31I	GGTCTC	3 cut(s)	208, 1596, 3125
Eco32I	GATATC	1 cut(s)	1852
Eco47I	GGWCC	6 cut(s)	136, 761, 1199, 1388, 2965, 3077
Eco53kI	GAGCTC	2 cut(s)	778, 1113
Eco57I	CTGAAG	2 cut(s)	140, 3197
Eco72I	CACGTG	1 cut(s)	1237
Eco88I	CYCGRG	4 cut(s)	113, 344, 427, 2870
Eco91I	GGTNACC	1 cut(s)	2546
EcoICRI	GAGCTC	2 cut(s)	778, 1113
EcoO109I	RGGNCCY	1 cut(s)	3077
EcoO65I	GGTNACC	1 cut(s)	2546
EcoRII	CCWGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
EcoRV	GATATC	1 cut(s)	1852
EcoT14I	CCWWGG	4 cut(s)	222, 968, 1752, 2035
EcoT22I	ATGCAT	1 cut(s)	1447
EcoT38I	GRGCYC	3 cut(s)	166, 780, 1115
EgeI	GGCGCC	1 cut(s)	966
EheI	GGCGCC	1 cut(s)	966
ErhI	CCWWGG	4 cut(s)	222, 968, 1752, 2035
FaeI	CATG	9 cut(s)	1520, 2492, 2555, 2687, 2705, 2831, 2987, 3022, 3058
FatI	CATG	9 cut(s)	1516, 2488, 2551, 2683, 2701, 2827, 2983, 3018, 3054
FauI	CCCGC	4 cut(s)	778, 1720, 1863, 2724
FbaI	TGATCA	1 cut(s)	178
FblI	GTMKAC	2 cut(s)	155, 1958
Fnu4HI	GCNGC	8 cut(s)	172, 314, 1094, 1921, 2062, 2079, 2807, 2933
FokI	GGATG	9 cut(s)	698, 749, 902, 1190, 1619, 1667, 1910, 2167, 2168
FriOI	GRGCYC	3 cut(s)	166, 780, 1115
Fsp4HI	GCNGC	8 cut(s)	172, 314, 1094, 1921, 2062, 2079, 2807, 2933
FspBI	CTAG	6 cut(s)	744, 1344, 1788, 2631, 2802, 3202
GlaI	GCGC	2 cut(s)	966, 2082
GluI	GCNGC	8 cut(s)	172, 314, 1094, 1921, 2062, 2079, 2807, 2933
GsaI	CCCAGC	1 cut(s)	2813
GsuI	CTGGAG	3 cut(s)	1077, 1626, 2409
HaeII	RGCGCY	1 cut(s)	968
HaeIII	GGCC	7 cut(s)	15, 164, 554, 987, 1557, 1920, 3148
HapII	CCGG	4 cut(s)	75, 488, 551, 2099
HgaI	GACGC	1 cut(s)	667
HhaI	GCGC	2 cut(s)	967, 2083
Hin1I	GRCGYC	1 cut(s)	965
Hin1II	CATG	9 cut(s)	1520, 2492, 2555, 2687, 2705, 2831, 2987, 3022, 3058
Hin6I	GCGC	2 cut(s)	965, 2081
HinP1I	GCGC	2 cut(s)	965, 2081
HincII	GTYRAC	2 cut(s)	156, 871
HindII	GTYRAC	2 cut(s)	156, 871
HindIII	AAGCTT	2 cut(s)	266, 1133
HpaI	GTTAAC	1 cut(s)	871
HpaII	CCGG	4 cut(s)	75, 488, 551, 2099
HphI	GGTGA	7 cut(s)	173, 264, 347, 1042, 1859, 2109, 2527
Hpy166II	GTNNAC	7 cut(s)	156, 704, 871, 1033, 1470, 1959, 2914
Hpy8I	GTNNAC	7 cut(s)	156, 704, 871, 1033, 1470, 1959, 2914
Hpy99I	CGWCG	1 cut(s)	160
HpyCH4IV	ACGT	5 cut(s)	158, 1236, 2330, 2862, 3048
HpyF10VI	GCNNNNNNNGC	5 cut(s)	21, 971, 1451, 1584, 2420
HpySE526I	ACGT	5 cut(s)	158, 1236, 2330, 2862, 3048
Hsp92I	GRCGYC	1 cut(s)	965
Hsp92II	CATG	9 cut(s)	1520, 2492, 2555, 2687, 2705, 2831, 2987, 3022, 3058
HspAI	GCGC	2 cut(s)	965, 2081
KasI	GGCGCC	1 cut(s)	964
Ksp22I	TGATCA	1 cut(s)	178
KspAI	GTTAAC	1 cut(s)	871
LmnI	GCTCC	4 cut(s)	920, 1811, 2149, 2428
Lsp1109I	GCAGC	6 cut(s)	183, 300, 1080, 2065, 2793, 2919
LweI	GCATC	7 cut(s)	1073, 1597, 1932, 2189, 2204, 2559, 3090
MabI	ACCWGGT	1 cut(s)	2529
MaeI	CTAG	6 cut(s)	744, 1344, 1788, 2631, 2802, 3202
MaeII	ACGT	5 cut(s)	158, 1236, 2330, 2862, 3048
MaeIII	GTNAC	7 cut(s)	651, 1373, 1800, 2372, 2533, 2546, 2918
MfeI	CAATTG	2 cut(s)	1000, 2169
MflI	RGATCY	6 cut(s)	542, 1252, 1645, 1949, 2627, 2636
MhlI	GDGCHC	7 cut(s)	166, 780, 1115, 1232, 1242, 2146, 2323
Mly113I	GGCGCC	1 cut(s)	965
MlyI	GAGTC	4 cut(s)	206, 826, 1272, 1593
MmeI	TCCRAC	5 cut(s)	114, 342, 381, 1420, 2440
Mph1103I	ATGCAT	1 cut(s)	1447
MroXI	GAANNNNTTC	3 cut(s)	268, 1049, 1466
MslI	CAYNNNNRTG	4 cut(s)	591, 2556, 2733, 2988
MspA1I	CMGCKG	2 cut(s)	90, 773
MspI	CCGG	4 cut(s)	75, 488, 551, 2099
MspR9I	CCNGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
MunI	CAATTG	2 cut(s)	1000, 2169
Mva1269I	GAATGC	3 cut(s)	693, 853, 2779
MvaI	CCWGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
MvnI	CGCG	1 cut(s)	2719
MwoI	GCNNNNNNNGC	5 cut(s)	21, 971, 1451, 1584, 2420
NarI	GGCGCC	1 cut(s)	965
NheI	GCTAGC	1 cut(s)	1787
NlaIII	CATG	9 cut(s)	1520, 2492, 2555, 2687, 2705, 2831, 2987, 3022, 3058
NmeAIII	GCCGAG	1 cut(s)	441
NsiI	ATGCAT	1 cut(s)	1447
NspI	RCATGY	2 cut(s)	2831, 3022
NspV	TTCGAA	1 cut(s)	500
PaeI	GCATGC	2 cut(s)	2831, 3022
PaeR7I	CTCGAG	1 cut(s)	113
PceI	AGGCCT	1 cut(s)	3148
PcsI	WCGNNNNNNNCGW	1 cut(s)	2868
PctI	GAATGC	3 cut(s)	693, 853, 2779
PdmI	GAANNNNTTC	3 cut(s)	268, 1049, 1466
PflMI	CCANNNNNTGG	2 cut(s)	1337, 1649
PfoI	TCCNGGA	2 cut(s)	2146, 2425
PkrI	GCNGC	8 cut(s)	173, 315, 1095, 1922, 2063, 2080, 2808, 2934
PleI	GAGTC	4 cut(s)	206, 825, 1271, 1593
PluTI	GGCGCC	1 cut(s)	968
PmaCI	CACGTG	1 cut(s)	1237
PmlI	CACGTG	1 cut(s)	1237
PpsI	GAGTC	4 cut(s)	206, 825, 1271, 1593
Ppu21I	YACGTR	2 cut(s)	1237, 2863
PpuMI	RGGWCCY	1 cut(s)	3077
Psp124BI	GAGCTC	2 cut(s)	780, 1115
Psp5II	RGGWCCY	1 cut(s)	3077
Psp6I	CCWGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
PspCI	CACGTG	1 cut(s)	1237
PspEI	GGTNACC	1 cut(s)	2546
PspFI	CCCAGC	1 cut(s)	2809
PspGI	CCWGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
PspOMI	GGGCCC	1 cut(s)	162
PspPI	GGNCC	8 cut(s)	136, 162, 163, 761, 1199, 1388, 2965, 3077
PspPPI	RGGWCCY	1 cut(s)	3077
PspXI	VCTCGAGB	1 cut(s)	113
PstI	CTGCAG	1 cut(s)	1098
PstNI	CAGNNNCTG	1 cut(s)	1102
PsuI	RGATCY	6 cut(s)	542, 1252, 1645, 1949, 2627, 2636
RsaI	GTAC	5 cut(s)	804, 1777, 2861, 3107, 3124
RsaNI	GTAC	5 cut(s)	803, 1776, 2860, 3106, 3123
RseI	CAYNNNNRTG	4 cut(s)	591, 2556, 2733, 2988
SacI	GAGCTC	2 cut(s)	780, 1115
SalI	GTCGAC	1 cut(s)	154
SatI	GCNGC	8 cut(s)	172, 314, 1094, 1921, 2062, 2079, 2807, 2933
Sau96I	GGNCC	8 cut(s)	136, 162, 163, 761, 1199, 1388, 2965, 3077
SchI	GAGTC	4 cut(s)	206, 826, 1272, 1593
ScrFI	CCNGG	7 cut(s)	193, 926, 2148, 2427, 2531, 2595, 2963
SduI	GDGCHC	7 cut(s)	166, 780, 1115, 1232, 1242, 2146, 2323
SexAI	ACCWGGT	1 cut(s)	2529
SfaNI	GCATC	7 cut(s)	1073, 1597, 1932, 2189, 2204, 2559, 3090
SfcI	CTRYAG	2 cut(s)	1094, 2023
SfoI	GGCGCC	1 cut(s)	966
Sfr274I	CTCGAG	1 cut(s)	113
SfuI	TTCGAA	1 cut(s)	500
SinI	GGWCC	6 cut(s)	136, 761, 1199, 1388, 2965, 3077
SlaI	CTCGAG	1 cut(s)	113
SmiMI	CAYNNNNRTG	4 cut(s)	591, 2556, 2733, 2988
SmlI	CTYRAG	5 cut(s)	113, 386, 581, 1681, 1739
SmoI	CTYRAG	5 cut(s)	113, 386, 581, 1681, 1739
SnaBI	TACGTA	1 cut(s)	2863
SphI	GCATGC	2 cut(s)	2831, 3022
SseBI	AGGCCT	1 cut(s)	3148
SspDI	GGCGCC	1 cut(s)	964
SspI	AATATT	2 cut(s)	622, 2341
SspMI	CTAG	6 cut(s)	744, 1344, 1788, 2631, 2802, 3202
SstI	GAGCTC	2 cut(s)	780, 1115
StuI	AGGCCT	1 cut(s)	3148
StyD4I	CCNGG	7 cut(s)	191, 924, 2146, 2425, 2529, 2593, 2961
StyI	CCWWGG	4 cut(s)	222, 968, 1752, 2035
TaiI	ACGT	5 cut(s)	161, 1239, 2333, 2865, 3051
TaqI	TCGA	6 cut(s)	114, 155, 215, 500, 815, 981
TatI	WGTACW	2 cut(s)	3105, 3122
TauI	GCSGC	2 cut(s)	1923, 2064
TscAI	CASTG	9 cut(s)	191, 791, 1060, 1180, 1186, 1230, 1390, 1477, 2563
TseI	GCWGC	6 cut(s)	171, 313, 1093, 2078, 2806, 2932
TspDTI	ATGAA	7 cut(s)	339, 852, 924, 1983, 2033, 3043, 3170
TspGWI	ACGGA	3 cut(s)	670, 819, 2324
TspRI	CASTG	9 cut(s)	191, 791, 1060, 1180, 1186, 1230, 1390, 1477, 2563
Van91I	CCANNNNNTGG	2 cut(s)	1337, 1649
VpaK11BI	GGWCC	6 cut(s)	136, 761, 1199, 1388, 2965, 3077
XbaI	TCTAGA	2 cut(s)	743, 2630
XceI	RCATGY	2 cut(s)	2831, 3022
XhoI	CTCGAG	1 cut(s)	113
XmiI	GTMKAC	2 cut(s)	155, 1958
XmnI	GAANNNNTTC	3 cut(s)	268, 1049, 1466
XspI	CTAG	6 cut(s)	744, 1344, 1788, 2631, 2802, 3202
Zsp2I	ATGCAT	1 cut(s)	1447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.