MD13G1064200.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007654
Physical Location & Seq
Forward (+)
4420753 .. 4427100
6348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1064200.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGGGTCGGAAAGAGGAGGCCTAGCTTTAGTCGTCACAGCATTTCTATTGGCTGTTGGGTTTCCAACATGTTTGTCGGATTCAGATATCAACAGGGGTAGCTTTCCCAAAGGTTTTGTTTTCGGAACTGCGTCTTCTGCTTTCCAGTACGAAGGTGCAGTTAAAGAGGACGGAAGGGGGCCTTCTATCTGGGACACCTTTTCACATACTTTTGGTAAAATAGCCGATTCCAGTAATGCAGATGTTGCTGTAGATGAGTATCACCGTTATAAGGATGTGCAACGTATGAAGGATATGGGAATGGATGCTTACAGATTTTCGATATCCTGGACTCGGATTTTCCCCAATGGAACCGGACACATTAATCAGGCGGGTGTTGATTACTACAATCGTCTCATCGATGCATTGCTAGCCAAAGGAATTGAACCGTATGTGACCCTCTATCACTGGGACCTCCCTCAAGCATTGGAAGACAAATACAGCGGGTGGCTCAACCCTCAAGTCATAAAGGACTTTGCAACATATGCGGACGCATGCTTTCAAAATTTTGGTGACAGGGTGAAGCACTGGATCACATTCAACGAGCCACATACATTTTCTATACAAGGATATGATGTAGGGCTCGAGGCACCGGGAAGGTGCTCTATCCCCCTTTTCCTTTTCTGCAGGGCAGGAAACTCGGCAACTGAGCCTTACATGGTTGCTCACAATGTCATCCTGTCTCATGGAACCGCGGCTGATATTTACCGGAGAAAGTACAAGTCAAAACAGCGTGGATCGGTTGGGGCATCATTTGATGTTATCTGGTATGAATCAGCAACAAACTCGACAGCAGACGTCATTGCAACTAAAATAGCCCAAGATTTTCAGCTTGGCTGGTTTCTTGATCCATTTATGTTTGGGGATTATCCAAGCTCTATGAGAAGTAGGGTTGGAAGTCGGCTGCCAACCTTTTCCAAATCCGAGTCTGCTCTAATTAAAGGGTCCTTGGATTTTGTGGGCATTAATCACTACACTACCTTCTATGGAAGCAACGATACCGCTGATATAATTGGACATCTACTCAATGACAGCCTTGCAGACTCTGGTGCCATTACTCTTCCATTCAAAGACGGAAAACCTATTGGCGATAGGGCGAATTCTATATGGTTATACATAGTGCCAGAGGGGATGAGAAAATTAATGAACTACATTAAGAAAAAGTACGGCAATCCTCTAGTGATTATCACTGAAAATGGCATGGATGACCCGAATAGCCCATTCATTTCTCTCAAGGATGCTCTAAAGGATACTAAAAGGATTAAATACCACCATGACTATCTTAAGAATTTGCTAGCTTCGATCAAAGAAGACGGCTGCAATGTGAAAGGCTATTTTGCTTGGTCTCTGCTGGATAATTGGGAATGGGCGGCTGGATACACTTCTCGATTTGGTCTCTATTTTGTGGATTATAAGGACAAGCTCAAGAGATACCCAAAGGACTCCGTTCAATGGTTCACGAAATTCTTGAATTCTACTCAAACAAGGCAATGA

Protein Analysis

511

Amino Acids

57.49

Weight (kDa)

7.18

Isoelectric Point (pI)

23.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 33 - 505 4.1e-156 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 270, 1452
AatII GACGTC 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 628, 1088
AccII CGCG 1 cut(s) 734
AciI CCGC 7 cut(s) 371, 483, 527, 732, 734, 1041, 1409
AclWI GGATC 3 cut(s) 578, 784, 881
AcsI RAATTY 5 cut(s) 544, 1138, 1327, 1502, 1510
AcyI GRCGYC 1 cut(s) 837
AfaI GTAC 3 cut(s) 149, 758, 1205
AfiI CCNNNNNNNGG 3 cut(s) 271, 333, 1491
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 6 cut(s) 425, 542, 580, 1108, 1490, 1510
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 6 cut(s) 26, 102, 871, 915, 1337, 1462
AluI AGCT 6 cut(s) 26, 102, 871, 915, 1337, 1462
Alw21I GWGCWC 1 cut(s) 644
Alw26I GTCTC 4 cut(s) 398, 726, 1389, 1439
AlwI GGATC 3 cut(s) 578, 784, 881
AlwNI CAGNNNCTG 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 623
AoxI GGCC 2 cut(s) 19, 179
ApeKI GCWGC 2 cut(s) 943, 1356
ApoI RAATTY 5 cut(s) 544, 1138, 1327, 1502, 1510
AseI ATTAAT 3 cut(s) 363, 1005, 1181
AspS9I GGNCC 3 cut(s) 179, 451, 984
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 3 cut(s) 254, 563, 571
AsuNHI GCTAGC 2 cut(s) 409, 1333
AvaI CYCGRG 1 cut(s) 623
AvaII GGWCC 2 cut(s) 451, 984
BanI GGYRCC 2 cut(s) 628, 1088
BanII GRGCYC 1 cut(s) 624
BarI GAAGNNNNNNTAC 2 cut(s) 1021, 1053
BbsI GAAGAC 3 cut(s) 126, 477, 1356
Bbv12I GWGCWC 1 cut(s) 644
BbvI GCAGC 2 cut(s) 930, 1343
BceAI ACGGC 2 cut(s) 1222, 1369
BcgI CGANNNNNNTGC 4 cut(s) 660, 694, 807, 841
BciT130I CCWGG 1 cut(s) 328
BciVI GTATCC 2 cut(s) 1282, 1409
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 4 cut(s) 398, 726, 1389, 1439
BfaI CTAG 4 cut(s) 23, 410, 1217, 1334
BfmI CTRYAG 2 cut(s) 249, 664
BfrI CTTAAG 1 cut(s) 1322
BfuI GTATCC 2 cut(s) 1282, 1409
BisI GCNGC 4 cut(s) 735, 944, 1357, 1410
BlsI GCNGC 4 cut(s) 736, 945, 1358, 1411
Bme1390I CCNGG 2 cut(s) 328, 633
Bme18I GGWCC 2 cut(s) 451, 984
BmeT110I CYCGRG 1 cut(s) 623
BmgT120I GGNCC 3 cut(s) 179, 451, 984
BmiI GGNNCC 7 cut(s) 180, 352, 452, 630, 730, 985, 1090
BmrFI CCNGG 2 cut(s) 328, 633
BmrI ACTGGG 1 cut(s) 457
BmsI GCATC 9 cut(s) 163, 295, 391, 492, 665, 783, 797, 1267, 1313
BmtI GCTAGC 2 cut(s) 413, 1337
BmuI ACTGGG 1 cut(s) 457
BpiI GAAGAC 3 cut(s) 126, 477, 1356
BpuEI CTTGAG 4 cut(s) 444, 483, 1256, 1448
BpuMI CCSGG 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 837
BsaI GGTCTC 2 cut(s) 1389, 1439
BsaJI CCNNGG 2 cut(s) 732, 987
BsaWI WCCGGW 2 cut(s) 353, 747
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 333, 1491
Bse1I ACTGG 4 cut(s) 145, 231, 452, 572
Bse3DI GCAATG 3 cut(s) 404, 840, 1366
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 732, 987
BseGI GGATG 6 cut(s) 280, 310, 714, 1176, 1249, 1282
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 271, 333, 1491
BseMI GCAATG 3 cut(s) 404, 840, 1366
BseMII CTCAG 1 cut(s) 678
BseNI ACTGG 4 cut(s) 145, 231, 452, 572
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 930, 1343
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 734
BshFI GGCC 2 cut(s) 21, 181
BshNI GGYRCC 2 cut(s) 628, 1088
BshVI ATCGAT 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 644
BsiHKCI CYCGRG 1 cut(s) 623
BsiSI CCGG 3 cut(s) 354, 632, 748
BslFI GGGAC 2 cut(s) 206, 464
BslI CCNNNNNNNGG 3 cut(s) 271, 333, 1491
BsmAI GTCTC 4 cut(s) 398, 726, 1389, 1439
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 206, 464
BsnI GGCC 2 cut(s) 21, 181
Bso31I GGTCTC 2 cut(s) 1389, 1439
BsoBI CYCGRG 1 cut(s) 623
Bsp1286I GDGCHC 2 cut(s) 624, 644
Bsp143I GATC 4 cut(s) 570, 776, 886, 1341
BspACI CCGC 7 cut(s) 371, 483, 527, 732, 734, 1041, 1409
BspANI GGCC 2 cut(s) 21, 181
BspCNI CTCAG 1 cut(s) 679
BspDI ATCGAT 1 cut(s) 399
BspFNI CGCG 1 cut(s) 734
BspLI GGNNCC 7 cut(s) 180, 352, 452, 630, 730, 985, 1090
BspMAI CTGCAG 1 cut(s) 668
BspOI GCTAGC 2 cut(s) 413, 1337
BspPI GGATC 3 cut(s) 578, 784, 881
BspT107I GGYRCC 2 cut(s) 628, 1088
BspTI CTTAAG 1 cut(s) 1322
BspTNI GGTCTC 2 cut(s) 1389, 1439
BsrDI GCAATG 3 cut(s) 404, 840, 1366
BsrI ACTGG 4 cut(s) 145, 231, 452, 572
BssECI CCNNGG 2 cut(s) 732, 987
BssMI GATC 4 cut(s) 570, 776, 886, 1341
BssNI GRCGYC 1 cut(s) 837
BssT1I CCWWGG 1 cut(s) 987
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 2 cut(s) 266, 429
Bst6I CTCTTC 1 cut(s) 1104
BstACI GRCGYC 1 cut(s) 837
BstAFI CTTAAG 1 cut(s) 1322
BstAPI GCANNNNNTGC 2 cut(s) 245, 524
BstC8I GCNNGC 3 cut(s) 411, 535, 1335
BstDEI CTNAG 1 cut(s) 687
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 6 cut(s) 280, 310, 714, 1176, 1249, 1282
BstFNI CGCG 1 cut(s) 734
BstKTI GATC 4 cut(s) 573, 779, 889, 1344
BstMAI GTCTC 4 cut(s) 398, 726, 1389, 1439
BstMBI GATC 4 cut(s) 570, 776, 886, 1341
BstMWI GCNNNNNNNGC 4 cut(s) 137, 245, 410, 524
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 2 cut(s) 72, 537
BstSCI CCNGG 2 cut(s) 326, 631
BstSFI CTRYAG 2 cut(s) 249, 664
BstUI CGCG 1 cut(s) 734
BstV1I GCAGC 2 cut(s) 930, 1343
BstV2I GAAGAC 3 cut(s) 126, 477, 1356
Bsu15I ATCGAT 1 cut(s) 399
BsuI GTATCC 2 cut(s) 1282, 1409
BsuRI GGCC 2 cut(s) 21, 181
BsuTUI ATCGAT 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 6 cut(s) 280, 310, 714, 1176, 1249, 1282
BtsIMutI CAGTG 3 cut(s) 445, 565, 1227
Cac8I GCNNGC 3 cut(s) 411, 535, 1335
CaiI CAGNNNCTG 1 cut(s) 1085
Cfr13I GGNCC 3 cut(s) 179, 451, 984
Cfr42I CCGCGG 1 cut(s) 735
ClaI ATCGAT 1 cut(s) 399
CseI GACGC 2 cut(s) 120, 539
Csp6I GTAC 3 cut(s) 148, 757, 1204
CviAII CATG 6 cut(s) 69, 534, 697, 725, 1240, 1313
CviQI GTAC 3 cut(s) 148, 757, 1204
DdeI CTNAG 1 cut(s) 687
DpnI GATC 4 cut(s) 572, 778, 888, 1343
DpnII GATC 4 cut(s) 570, 776, 886, 1341
Eam1104I CTCTTC 1 cut(s) 1104
EarI CTCTTC 1 cut(s) 1104
Eco130I CCWWGG 1 cut(s) 987
Eco147I AGGCCT 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 2 cut(s) 1389, 1439
Eco32I GATATC 2 cut(s) 88, 324
Eco47I GGWCC 2 cut(s) 451, 984
Eco88I CYCGRG 1 cut(s) 623
EcoO109I RGGNCCY 3 cut(s) 179, 451, 984
EcoRI GAATTC 2 cut(s) 1138, 1510
EcoRII CCWGG 1 cut(s) 326
EcoRV GATATC 2 cut(s) 88, 324
EcoT14I CCWWGG 1 cut(s) 987
EcoT22I ATGCAT 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 987
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 6 cut(s) 72, 537, 700, 728, 1243, 1316
FalI AAGNNNNNCTT 2 cut(s) 166, 198
FaqI GGGAC 2 cut(s) 206, 464
FatI CATG 6 cut(s) 68, 533, 696, 724, 1239, 1312
FauI CCCGC 2 cut(s) 364, 476
FauNDI CATATG 1 cut(s) 523
Fnu4HI GCNGC 4 cut(s) 735, 944, 1357, 1410
FokI GGATG 6 cut(s) 287, 317, 701, 1183, 1256, 1289
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 4 cut(s) 735, 944, 1357, 1410
FspBI CTAG 4 cut(s) 23, 410, 1217, 1334
GluI GCNGC 4 cut(s) 735, 944, 1357, 1410
HaeIII GGCC 2 cut(s) 21, 181
HapII CCGG 3 cut(s) 354, 632, 748
HgaI GACGC 2 cut(s) 120, 539
Hin1I GRCGYC 1 cut(s) 837
Hin1II CATG 6 cut(s) 72, 537, 700, 728, 1243, 1316
HinfI GANTC 7 cut(s) 80, 227, 331, 812, 965, 1082, 1481
HpaII CCGG 3 cut(s) 354, 632, 748
HphI GGTGA 3 cut(s) 254, 563, 571
Hpy166II GTNNAC 1 cut(s) 1497
Hpy188I TCNGA 6 cut(s) 10, 79, 85, 125, 336, 964
Hpy188III TCNNGA 5 cut(s) 884, 1425, 1465, 1498, 1507
Hpy8I GTNNAC 1 cut(s) 1497
HpyAV CCTTC 6 cut(s) 146, 168, 192, 283, 630, 1030
HpyCH4III ACNGT 2 cut(s) 266, 429
HpyCH4IV ACGT 2 cut(s) 283, 837
HpyCH4V TGCA 9 cut(s) 158, 239, 280, 404, 518, 666, 845, 1079, 1359
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 245, 410, 524
HpyF3I CTNAG 1 cut(s) 687
HpySE526I ACGT 2 cut(s) 283, 837
Hsp92I GRCGYC 1 cut(s) 837
Hsp92II CATG 6 cut(s) 72, 537, 700, 728, 1243, 1316
KspI CCGCGG 1 cut(s) 735
Kzo9I GATC 4 cut(s) 570, 776, 886, 1341
Lsp1109I GCAGC 2 cut(s) 930, 1343
LweI GCATC 4 cut(s) 295, 391, 797, 1267
MaeI CTAG 4 cut(s) 23, 410, 1217, 1334
MaeII ACGT 2 cut(s) 283, 837
MaeIII GTNAC 3 cut(s) 34, 433, 551
MalI GATC 4 cut(s) 572, 778, 888, 1343
MboI GATC 4 cut(s) 570, 776, 886, 1341
MboII GAAGA 4 cut(s) 126, 482, 1091, 1361
MhlI GDGCHC 2 cut(s) 624, 644
MlyI GAGTC 4 cut(s) 325, 974, 1076, 1475
MmeI TCCRAC 3 cut(s) 57, 89, 913
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 8 cut(s) 162, 363, 978, 1005, 1181, 1194, 1302, 1323
MspA1I CMGCKG 3 cut(s) 483, 734, 1043
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 3 cut(s) 354, 632, 748
MspR9I CCNGG 2 cut(s) 328, 633
MvaI CCWGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 4 cut(s) 137, 245, 410, 524
NciI CCSGG 1 cut(s) 633
NdeI CATATG 1 cut(s) 523
NdeII GATC 4 cut(s) 570, 776, 886, 1341
NheI GCTAGC 2 cut(s) 409, 1333
NlaIII CATG 6 cut(s) 72, 537, 700, 728, 1243, 1316
NlaIV GGNNCC 7 cut(s) 180, 352, 452, 630, 730, 985, 1090
NmeAIII GCCGAG 1 cut(s) 659
NmuCI GTSAC 3 cut(s) 34, 433, 551
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 2 cut(s) 72, 537
PaeI GCATGC 1 cut(s) 537
PaeR7I CTCGAG 1 cut(s) 623
PaqCI CACCTGC 2 cut(s) 486, 710
PceI AGGCCT 1 cut(s) 21
PciI ACATGT 1 cut(s) 68
PfeI GAWTC 3 cut(s) 80, 227, 812
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 4 cut(s) 736, 945, 1358, 1411
PleI GAGTC 4 cut(s) 325, 973, 1076, 1475
PpsI GAGTC 4 cut(s) 325, 973, 1076, 1475
PpuMI RGGWCCY 2 cut(s) 451, 984
PscI ACATGT 1 cut(s) 68
PshBI ATTAAT 3 cut(s) 363, 1005, 1181
PsiI TTATAA 2 cut(s) 270, 1452
Psp5II RGGWCCY 2 cut(s) 451, 984
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 7 cut(s) 180, 352, 452, 630, 730, 985, 1090
PspPI GGNCC 3 cut(s) 179, 451, 984
PspPPI RGGWCCY 2 cut(s) 451, 984
PspXI VCTCGAGB 1 cut(s) 623
PstI CTGCAG 1 cut(s) 668
PstNI CAGNNNCTG 1 cut(s) 1085
RsaI GTAC 3 cut(s) 149, 758, 1205
RsaNI GTAC 3 cut(s) 148, 757, 1204
SacII CCGCGG 1 cut(s) 735
SaqAI TTAA 8 cut(s) 162, 363, 978, 1005, 1181, 1194, 1302, 1323
SatI GCNGC 4 cut(s) 735, 944, 1357, 1410
Sau3AI GATC 4 cut(s) 570, 776, 886, 1341
Sau96I GGNCC 3 cut(s) 179, 451, 984
SchI GAGTC 4 cut(s) 325, 974, 1076, 1475
ScrFI CCNGG 2 cut(s) 328, 633
SduI GDGCHC 2 cut(s) 624, 644
SfaNI GCATC 4 cut(s) 295, 391, 797, 1267
SfcI CTRYAG 2 cut(s) 249, 664
Sfr274I CTCGAG 1 cut(s) 623
Sfr303I CCGCGG 1 cut(s) 735
SgrBI CCGCGG 1 cut(s) 735
SinI GGWCC 2 cut(s) 451, 984
SlaI CTCGAG 1 cut(s) 623
SmlI CTYRAG 6 cut(s) 459, 498, 623, 1271, 1322, 1463
SmoI CTYRAG 6 cut(s) 459, 498, 623, 1271, 1322, 1463
SphI GCATGC 1 cut(s) 537
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 7 cut(s) 371, 483, 527, 732, 734, 1041, 1409
SspMI CTAG 4 cut(s) 23, 410, 1217, 1334
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 326, 631
StyI CCWWGG 1 cut(s) 987
TaaI ACNGT 2 cut(s) 266, 429
TaiI ACGT 2 cut(s) 286, 840
TaqI TCGA 6 cut(s) 320, 399, 624, 827, 1340, 1426
TatI WGTACW 1 cut(s) 756
TauI GCSGC 2 cut(s) 737, 1412
TfiI GAWTC 3 cut(s) 80, 227, 812
Tru1I TTAA 8 cut(s) 162, 363, 978, 1005, 1181, 1194, 1302, 1323
Tru9I TTAA 8 cut(s) 162, 363, 978, 1005, 1181, 1194, 1302, 1323
TscAI CASTG 3 cut(s) 452, 572, 1234
TseFI GTSAC 3 cut(s) 34, 433, 551
TseI GCWGC 2 cut(s) 943, 1356
Tsp45I GTSAC 3 cut(s) 34, 433, 551
TspDTI ATGAA 4 cut(s) 302, 825, 1199, 1252
TspGWI ACGGA 3 cut(s) 186, 1128, 1474
TspRI CASTG 3 cut(s) 452, 572, 1234
Vha464I CTTAAG 1 cut(s) 1322
VpaK11BI GGWCC 2 cut(s) 451, 984
VspI ATTAAT 3 cut(s) 363, 1005, 1181
XapI RAATTY 5 cut(s) 544, 1138, 1327, 1502, 1510
XceI RCATGY 2 cut(s) 72, 537
XhoI CTCGAG 1 cut(s) 623
XspI CTAG 4 cut(s) 23, 410, 1217, 1334
ZraI GACGTC 1 cut(s) 838
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.