Rroxscaffold_5G00379600

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007654
Physical Location & Seq
Forward (+)
59893755 .. 59898096
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379600.1

Sequence Viewer

Length: 1278 bp
ATGCAGATGTTGCTGTGGATCAGTATCACCTTTATGATGGATGTGCAACTTATGAAGGATATGGGAATGGATGCTTATAGGTTTTCGATATCCTGGTCTCGGATTTTCCCCAATGGAACGGGGCAAATCAATCAGGCAGGGGTTGATCACTACAATAAACTCATCAATTCATTGCTAGCGAAAGGAATTGAACCGTATGTAACCCTCTACCACTGGGATCTTCCTCAAGCCTTGGAAGACAGATACACTGGGTGGCTTGACGCTCAAATCATAAAGGACTTTGCAGTATATGCGGAGACATGCTTTCAGAAATTTGGTGACAGGGTCAAGCACTGGATCACATTCAACGAGCCACACACATTTGCTGTGCAGGGATATGATGTAGGTCTCCAGGCACCAGGAAGATGCTCTATCCTACTGCACCTTTTCTGTAGGGCCGGAAACTCTGCGACTGAGCCTTACATAGTTGCTCACAATGTCATCCTTTCTCATGCAACTGTTGCCGACATTTACAGGAAAAAGTATAAGTCAAAACAAAAAGGATCAATTGGGGCATCATTTGATGTTATTTGGTATCGATCAGCAACAAACTCCACAGCAGACATTGAAGCAACTCAGAGAGCCCAAGATTTTCAGCTTGGCTGGTTTCTTGACCCATTTATGTTTGGGGATTATCCATTCTCCATGAGAAGTAGAGTTGGAAGCCGGCTGCCAAAGTTTTCCAAATCTGAGTCTACTTTAATCAAGGGGTCATTGGATTTTGTGGGCATTAATCACTACACTACTTTCTATGCCAGTAACAATTCTATAGTCGGACTACTGAATGACTCCCTATCAGACTCTGGTGCCATTACCCTTCCTTTCAAAGATGGGAAACCTATTGGAGATAAGGCAAATTCGATATGGTTATACATAGTGCCTGAGGGGATGAGAAGCTTAATGAACTACATCAAGCAAAAGTATGGCAATCCTCCGGTGATGATCACTGAGAATGGCATGGATGACCCGAATAGCCCATTCATTTCCCTCAAGGATGCTCTAAAGGACAAGAAAAGGATCAATTACCACCGTGACTATTTGTCAAACTTGCTAGCTTCAATCAAAGAAGATGGGTGCAATGTGAGCGGTTATTTTGCATGGTCTCTACTGGATAATTGGGAGTGGGCTGCGGGATACACTTCAAGATTTGGTCTCTATTTCATTGACTATAAGGACAAGCTCAAGAGATACCCTAAGGACTCTGTTCAATGGTTCAAGAACTTCTTAACTTCTACTTGA

Protein Analysis

425

Amino Acids

48.63

Weight (kDa)

8.58

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 12 - 423 1e-130 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 394, 845
AccBSI CCGCTC 1 cut(s) 1125
AccI GTMKAC 1 cut(s) 734
AciI CCGC 3 cut(s) 293, 1125, 1169
AclWI GGATC 5 cut(s) 26, 225, 344, 550, 1064
AcsI RAATTY 2 cut(s) 311, 895
AdeI CACNNNGTG 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 99
AgsI TTSAA 8 cut(s) 191, 346, 608, 865, 1098, 1182, 1247, 1255
AhdI GACNNNNNGTC 1 cut(s) 1078
AjnI CCWGG 3 cut(s) 92, 390, 397
AluBI AGCT 4 cut(s) 637, 936, 1094, 1219
AluI AGCT 4 cut(s) 637, 936, 1094, 1219
Alw26I GTCTC 5 cut(s) 102, 290, 392, 1146, 1196
AlwI GGATC 5 cut(s) 26, 225, 344, 550, 1064
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 709, 1166
ApoI RAATTY 2 cut(s) 311, 895
AseI ATTAAT 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 19, 329, 988
AsuNHI GCTAGC 2 cut(s) 175, 1090
AxyI CCTNAGG 2 cut(s) 921, 1233
BanI GGYRCC 2 cut(s) 394, 845
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 696, 1153
BccI CCATC 3 cut(s) 31, 863, 1103
BciT130I CCWGG 3 cut(s) 94, 392, 399
BciVI GTATCC 1 cut(s) 1166
BclI TGATCA 2 cut(s) 145, 981
BcoDI GTCTC 5 cut(s) 102, 290, 392, 1146, 1196
BfaI CTAG 2 cut(s) 176, 1091
BfmI CTRYAG 2 cut(s) 430, 807
BfuI GTATCC 1 cut(s) 1166
BisI GCNGC 2 cut(s) 710, 1167
BlsI GCNGC 2 cut(s) 711, 1168
Bme1390I CCNGG 3 cut(s) 94, 392, 399
BmeRI GACNNNNNGTC 1 cut(s) 1078
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 396, 847
BmrFI CCNGG 3 cut(s) 94, 392, 399
BmrI ACTGGG 2 cut(s) 223, 258
BmsI GCATC 4 cut(s) 61, 395, 563, 1024
BmtI GCTAGC 2 cut(s) 179, 1094
BmuI ACTGGG 2 cut(s) 223, 258
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 374
BpuEI CTTGAG 3 cut(s) 210, 1013, 1205
Bsa29I ATCGAT 1 cut(s) 577
BsaBI GATNNNNATC 1 cut(s) 23
BsaI GGTCTC 4 cut(s) 102, 392, 1146, 1196
BsaJI CCNNGG 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 705
Bse1I ACTGG 5 cut(s) 218, 253, 338, 795, 1152
Bse21I CCTNAGG 2 cut(s) 921, 1233
Bse3DI GCAATG 2 cut(s) 170, 1123
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 3 cut(s) 94, 392, 399
BseCI ATCGAT 1 cut(s) 577
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 6 cut(s) 46, 76, 480, 933, 1006, 1039
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMI GCAATG 2 cut(s) 170, 1123
BseMII CTCAG 5 cut(s) 444, 629, 720, 912, 978
BseNI ACTGG 5 cut(s) 218, 253, 338, 795, 1152
BseXI GCAGC 2 cut(s) 696, 1153
BsgI GTGCAG 2 cut(s) 389, 404
BshFI GGCC 1 cut(s) 437
BshNI GGYRCC 2 cut(s) 394, 845
BshVI ATCGAT 1 cut(s) 577
BsiSI CCGG 3 cut(s) 438, 706, 974
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 5 cut(s) 102, 290, 392, 1146, 1196
BsnI GGCC 1 cut(s) 437
Bso31I GGTCTC 4 cut(s) 102, 392, 1146, 1196
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
BspACI CCGC 3 cut(s) 293, 1125, 1169
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 5 cut(s) 445, 628, 721, 913, 979
BspDI ATCGAT 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 396, 847
BspOI GCTAGC 2 cut(s) 179, 1094
BspPI GGATC 5 cut(s) 26, 225, 344, 550, 1064
BspT107I GGYRCC 2 cut(s) 394, 845
BspTNI GGTCTC 4 cut(s) 102, 392, 1146, 1196
BsrBI CCGCTC 1 cut(s) 1125
BsrDI GCAATG 2 cut(s) 170, 1123
BsrFI RCCGGY 1 cut(s) 705
BsrI ACTGG 5 cut(s) 218, 253, 338, 795, 1152
BssAI RCCGGY 1 cut(s) 705
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 3 cut(s) 94, 392, 399
Bst4CI ACNGT 3 cut(s) 195, 499, 1070
BstAPI GCANNNNNTGC 3 cut(s) 10, 290, 500
BstC8I GCNNGC 3 cut(s) 177, 707, 1092
BstDEI CTNAG 6 cut(s) 453, 615, 729, 921, 987, 1233
BstF5I GGATG 6 cut(s) 46, 76, 480, 933, 1006, 1039
BstKTI GATC 8 cut(s) 21, 148, 220, 339, 545, 581, 984, 1059
BstMAI GTCTC 5 cut(s) 102, 290, 392, 1146, 1196
BstMBI GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
BstMWI GCNNNNNNNGC 4 cut(s) 10, 290, 500, 1122
BstNI CCWGG 3 cut(s) 94, 392, 399
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 3 cut(s) 92, 390, 397
BstSFI CTRYAG 2 cut(s) 430, 807
BstV1I GCAGC 2 cut(s) 696, 1153
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 577
Bsu36I CCTNAGG 2 cut(s) 921, 1233
BsuI GTATCC 1 cut(s) 1166
BsuRI GGCC 1 cut(s) 437
BsuTUI ATCGAT 1 cut(s) 577
BtsCI GGATG 6 cut(s) 46, 76, 480, 933, 1006, 1039
BtsIMutI CAGTG 4 cut(s) 211, 246, 331, 984
Cac8I GCNNGC 3 cut(s) 177, 707, 1092
CaiI CAGNNNCTG 1 cut(s) 842
Cfr10I RCCGGY 1 cut(s) 705
Cfr13I GGNCC 1 cut(s) 435
ClaI ATCGAT 1 cut(s) 577
CseI GACGC 1 cut(s) 269
CviAII CATG 5 cut(s) 300, 491, 685, 997, 1137
DdeI CTNAG 6 cut(s) 453, 615, 729, 921, 987, 1233
DpnI GATC 8 cut(s) 20, 147, 219, 338, 544, 580, 983, 1058
DpnII GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
DraIII CACNNNGTG 1 cut(s) 252
DriI GACNNNNNGTC 1 cut(s) 1078
Eam1105I GACNNNNNGTC 1 cut(s) 1078
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 4 cut(s) 102, 392, 1146, 1196
Eco32I GATATC 1 cut(s) 90
Eco81I CCTNAGG 2 cut(s) 921, 1233
EcoRII CCWGG 3 cut(s) 92, 390, 397
EcoRV GATATC 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 5 cut(s) 303, 494, 688, 1000, 1140
FalI AAGNNNNNCTT 1 cut(s) 1247
FatI CATG 5 cut(s) 299, 490, 684, 996, 1136
FauI CCCGC 1 cut(s) 1162
FbaI TGATCA 2 cut(s) 145, 981
FblI GTMKAC 1 cut(s) 734
Fnu4HI GCNGC 2 cut(s) 710, 1167
FokI GGATG 6 cut(s) 53, 83, 467, 940, 1013, 1046
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 2 cut(s) 710, 1167
FspBI CTAG 2 cut(s) 176, 1091
GluI GCNGC 2 cut(s) 710, 1167
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 1 cut(s) 437
HapII CCGG 3 cut(s) 438, 706, 974
HgaI GACGC 1 cut(s) 269
Hin1II CATG 5 cut(s) 303, 494, 688, 1000, 1140
HindIII AAGCTT 1 cut(s) 934
HinfI GANTC 4 cut(s) 731, 827, 839, 1238
HpaII CCGG 3 cut(s) 438, 706, 974
HphI GGTGA 3 cut(s) 19, 329, 988
Hpy166II GTNNAC 1 cut(s) 735
Hpy188I TCNGA 6 cut(s) 102, 309, 618, 730, 815, 838
Hpy188III TCNNGA 4 cut(s) 650, 1182, 1222, 1255
Hpy8I GTNNAC 1 cut(s) 735
HpyAV CCTTC 2 cut(s) 49, 866
HpyCH4III ACNGT 3 cut(s) 195, 499, 1070
HpyCH4V TGCA 8 cut(s) 4, 46, 284, 370, 421, 494, 1116, 1136
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 290, 500, 1122
HpyF3I CTNAG 6 cut(s) 453, 615, 729, 921, 987, 1233
Hsp92II CATG 5 cut(s) 303, 494, 688, 1000, 1140
KroI GCCGGC 1 cut(s) 705
KroNI GCCGGC 1 cut(s) 707
Ksp22I TGATCA 2 cut(s) 145, 981
Kzo9I GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
Lsp1109I GCAGC 2 cut(s) 696, 1153
LweI GCATC 4 cut(s) 61, 395, 563, 1024
MaeI CTAG 2 cut(s) 176, 1091
MaeIII GTNAC 4 cut(s) 199, 317, 797, 1070
MalI GATC 8 cut(s) 20, 147, 219, 338, 544, 580, 983, 1058
MbiI CCGCTC 1 cut(s) 1125
MboI GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
MboII GAAGA 4 cut(s) 212, 248, 414, 1118
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 8 cut(s) 166, 186, 311, 546, 802, 895, 1060, 1153
MlyI GAGTC 4 cut(s) 740, 821, 833, 1232
MmeI TCCRAC 2 cut(s) 679, 793
MnlI CCTC 5 cut(s) 215, 234, 916, 981, 1037
MroNI GCCGGC 1 cut(s) 705
MseI TTAA 4 cut(s) 740, 771, 938, 1265
MslI CAYNNNNRTG 1 cut(s) 32
MspI CCGG 3 cut(s) 438, 706, 974
MspR9I CCNGG 3 cut(s) 94, 392, 399
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 3 cut(s) 94, 392, 399
MwoI GCNNNNNNNGC 4 cut(s) 10, 290, 500, 1122
NaeI GCCGGC 1 cut(s) 707
NdeII GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
NgoMIV GCCGGC 1 cut(s) 705
NheI GCTAGC 2 cut(s) 175, 1090
NlaIII CATG 5 cut(s) 303, 494, 688, 1000, 1140
NlaIV GGNNCC 2 cut(s) 396, 847
NmuCI GTSAC 2 cut(s) 317, 1070
NspI RCATGY 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 707
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 2 cut(s) 711, 1168
PleI GAGTC 4 cut(s) 739, 821, 833, 1232
PpsI GAGTC 4 cut(s) 739, 821, 833, 1232
PshBI ATTAAT 1 cut(s) 771
Psp6I CCWGG 3 cut(s) 92, 390, 397
PspGI CCWGG 3 cut(s) 92, 390, 397
PspN4I GGNNCC 2 cut(s) 396, 847
PspPI GGNCC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 217
PsyI GACNNNGTC 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 4 cut(s) 740, 771, 938, 1265
SatI GCNGC 2 cut(s) 710, 1167
Sau3AI GATC 8 cut(s) 18, 145, 217, 336, 542, 578, 981, 1056
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 4 cut(s) 740, 821, 833, 1232
ScrFI CCNGG 3 cut(s) 94, 392, 399
SduI GDGCHC 1 cut(s) 625
SetI ASST 9 cut(s) 32, 83, 388, 426, 639, 880, 938, 1096, 1221
SfaNI GCATC 4 cut(s) 61, 395, 563, 1024
SfcI CTRYAG 2 cut(s) 430, 807
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 3 cut(s) 225, 1028, 1220
SmoI CTYRAG 3 cut(s) 225, 1028, 1220
Sse9I AATT 8 cut(s) 166, 186, 311, 546, 802, 895, 1060, 1153
SsiI CCGC 3 cut(s) 293, 1125, 1169
SspMI CTAG 2 cut(s) 176, 1091
StyD4I CCNGG 3 cut(s) 92, 390, 397
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 3 cut(s) 195, 499, 1070
TaqI TCGA 3 cut(s) 86, 577, 899
TasI AATT 8 cut(s) 166, 186, 311, 546, 802, 895, 1060, 1153
Tru1I TTAA 4 cut(s) 740, 771, 938, 1265
Tru9I TTAA 4 cut(s) 740, 771, 938, 1265
TscAI CASTG 4 cut(s) 218, 253, 338, 991
TseFI GTSAC 2 cut(s) 317, 1070
TseI GCWGC 2 cut(s) 709, 1166
Tsp45I GTSAC 2 cut(s) 317, 1070
TspDTI ATGAA 5 cut(s) 68, 159, 956, 1009, 1189
TspRI CASTG 4 cut(s) 218, 253, 338, 991
Tth111I GACNNNGTC 1 cut(s) 323
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 2 cut(s) 311, 895
XceI RCATGY 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 734
XspI CTAG 2 cut(s) 176, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.