Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007654

Physical Location & Seq

Genomic Coordinates Forward (+)

4457883 .. 4462522

Gene Structure 4640 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1062800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGAGGTCGGCAAGAGGAGGCGTTGCTATGGTGGTTACAGCATTTCTATTGGCTGTTGGGTTTCCAACATGTTTGTCAGCTTCGGATATCAACAGGGTTAGCTTTCCCAAAGATTTCGTTTTCGGGACTGCCTCTTCTGCTTTCCAGTACGAAGGTGCGGTTAAAGAGGATGGAAGGGGGCCTTCGATCTGGGACACATTTTCACATACTTTTGGTAAGATAGCTGATTTCAGCAATGCAGATGTTGCTTTGGATCAGTATCACCGTTATAAGGAAGATGTGCAACTTATGAAGGATATGGGACTAGATGCTTACAGATTTTCGATATCCTGGACTCGGATTTTTCCCAATGGAACCGGGCAAATTAATCAGGCGGGTGTTGATTACTACAATCGTCTCATTGATGCATTACTAGCCAAAGGAATTGAACCGTATGTGACCCTCTATCACTGGGACCTCCCTCAAGCCTTGGAAGACAGATACAACGGGTGGCTCAACCCTCAAATCATAAAGGACTTTGCAACGTACGCGGACACATGCTTTCAACATTTTGGTGACAGGGTGAAGCACTGGATCACATTCAACGAGCCACATACATTTTCTGTACATGGATATGCTTCGGGCCTCCAGGCACCGGGAAGGTGCTCTATCATGCGTCCGCTCTTATGCAGGTCCGGAAACTCTTCAACTGAGCCTTACATGGTTGCTCACAATGTCATCCTGTCTCACGGAACTGTGGCTGATATTTACAAGAGAAAGTATAAGTCAAAACAGCGGGGATCGGTTGGGGCATCATTTGATGTTATCTGGTACGAACCAGAAACAAACTCAACAGAAGACGTCATTGCAACTGACATAGCCCAAGAATTTCAGCTTGGCTGGTTTCTTGATCCATTTATTTTCGGGGATTATCCAAGCTCTATGAGAAGAAGGGTTGGGAGCCGGCTGCCAACCTTTTCCAAATCCGAGTCTACTCTAATTAAAGGGTCCTTGGATTTTGTCGGCATTAATCACTACACTACCTTCTATGGTAAAAACGATACCAGGGATTTAATCGGAGGTCTACTTAACGGCAGCCTTTCAGACTCTGGTGCCATTACCCTTCCATTCAAAAACTGGAAACCTATTGGAGATAGGGCGAATTCTATATGGTTATACATAGTCCCAGAGGGGATGAGAAAATTAATGAACTACATTAAGCAAAAGTACGGCAATCCTCCAGTGATTATCACCGAAAATGGCATGGATGACCCAAATAGCCAGTTCATTTCCCTCAAGGACGCTCTAAGGGATACAAAAAGGATTAAATACCACCATGACTATCTTGCAAATTTGCTAGCTTCAATCAAGCAAGACGGCTGCAATGTGATAGGCTATTTTGCCTGGTCTCTACTGGATAATTGGGAATGGACGGCTGGATTCACTTCTCGATTTGGTCTCTACTTTGTGGATTATAAGGACAAGCTCAAGAGATACCCGAAGGACTCCGTTCAATGGTTCACGAATTTCTTGAATTCTACTCAAACAAGGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008422 GO:0009507 GO:0009536 GO:0015925 GO:0015926 GO:0016787 GO:0016798 GO:0033907 GO:0042973 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0071704 GO:0080079 GO:0080083 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

512

Amino Acids

58.11

Weight (kDa)

8.08

Isoelectric Point (pI)

26.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	34 - 506	1.5e-161	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26560
fragaria_vesca	FvH4_4g30310 FvH4_4g30310 FvH4_4g30310 FvH4_4g30310 FvH4_4g30310 FvH4_4g30310 FvH4_4g30310
malus_domestica	MD13G1064200.v1.1 MD13G1064300.v1.1 MD16G1062800.v1.1
prunus_persica	Prupe.1G284600_v2.0.a1
pyrus_communis	pycom16g05490
rosa_chinensis	RchiOBHm_Chr4g0438611
rosa_laevigata	RLG00000006347
rosa_multiflora	Rmu_sc0002327.1_g000001
rosa_roxburghii	Rroxscaffold_5G00379600
rosa_rugosa	Rorug04G0311700 Rorug04G0311800
rosa_samantha	Rh4AG362200 Rh4BG373600 Rh4CG387500
rosa_wichuraiana	Rw0G006690 Rw0G016640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	270, 1455
AatII	GACGTC	1 cut(s)	843
Acc36I	ACCTGC	1 cut(s)	660
AccB1I	GGYRCC	2 cut(s)	631, 1091
AccBSI	CCGCTC	1 cut(s)	661
AccI	GTMKAC	2 cut(s)	971, 1063
AccII	CGCG	1 cut(s)	530
AccIII	TCCGGA	1 cut(s)	674
AciI	CCGC	5 cut(s)	158, 374, 530, 659, 775
AclWI	GGATC	4 cut(s)	261, 581, 787, 884
AcsI	RAATTY	5 cut(s)	866, 1141, 1330, 1504, 1513
AcyI	GRCGYC	1 cut(s)	840
AfaI	GTAC	5 cut(s)	149, 527, 606, 812, 1208
AfiI	CCNNNNNNNGG	4 cut(s)	271, 336, 634, 1494
AflIII	ACRYGT	1 cut(s)	68
AgsI	TTSAA	8 cut(s)	428, 545, 583, 687, 1111, 1344, 1493, 1513
AjnI	CCWGG	4 cut(s)	329, 627, 1043, 1382
AjuI	GAANNNNNNNTTGG	2 cut(s)	858, 890
AluBI	AGCT	7 cut(s)	80, 102, 224, 874, 918, 1340, 1465
AluI	AGCT	7 cut(s)	80, 102, 224, 874, 918, 1340, 1465
Alw21I	GWGCWC	1 cut(s)	647
Alw26I	GTCTC	4 cut(s)	401, 729, 1392, 1442
AlwI	GGATC	4 cut(s)	261, 581, 787, 884
AlwNI	CAGNNNCTG	1 cut(s)	1088
Aor13HI	TCCGGA	1 cut(s)	674
AoxI	GGCC	2 cut(s)	179, 622
ApeKI	GCWGC	3 cut(s)	946, 1074, 1359
ApoI	RAATTY	5 cut(s)	866, 1141, 1330, 1504, 1513
AseI	ATTAAT	3 cut(s)	366, 1008, 1184
Asp700I	GAANNNNTTC	1 cut(s)	682
AspS9I	GGNCC	5 cut(s)	179, 454, 622, 672, 987
AsuC2I	CCSGG	2 cut(s)	358, 636
AsuHPI	GGTGA	4 cut(s)	254, 566, 574, 1222
AsuNHI	GCTAGC	1 cut(s)	1336
AvaII	GGWCC	3 cut(s)	454, 672, 987
BanI	GGYRCC	2 cut(s)	631, 1091
BbsI	GAAGAC	2 cut(s)	480, 843
Bbv12I	GWGCWC	1 cut(s)	647
BbvI	GCAGC	3 cut(s)	933, 1086, 1346
BccI	CCATC	1 cut(s)	164
BceAI	ACGGC	4 cut(s)	1087, 1225, 1372, 1428
BciT130I	CCWGG	4 cut(s)	331, 629, 1045, 1384
BciVI	GTATCC	1 cut(s)	1285
BcnI	CCSGG	2 cut(s)	358, 636
BcoDI	GTCTC	4 cut(s)	401, 729, 1392, 1442
BfaI	CTAG	3 cut(s)	305, 413, 1337
BfuAI	ACCTGC	1 cut(s)	660
BfuI	GTATCC	1 cut(s)	1285
BisI	GCNGC	3 cut(s)	947, 1075, 1360
BlsI	GCNGC	3 cut(s)	948, 1076, 1361
Bme1390I	CCNGG	6 cut(s)	331, 358, 629, 636, 1045, 1384
Bme18I	GGWCC	3 cut(s)	454, 672, 987
BmgT120I	GGNCC	5 cut(s)	179, 454, 622, 672, 987
BmiI	GGNNCC	7 cut(s)	180, 355, 455, 633, 941, 988, 1093
BmrFI	CCNGG	6 cut(s)	331, 358, 629, 636, 1045, 1384
BmrI	ACTGGG	1 cut(s)	460
BmsI	GCATC	3 cut(s)	298, 394, 800
BmtI	GCTAGC	1 cut(s)	1340
BmuI	ACTGGG	1 cut(s)	460
BpiI	GAAGAC	2 cut(s)	480, 843
BpmI	CTGGAG	2 cut(s)	611, 1203
BpuEI	CTTGAG	3 cut(s)	447, 1259, 1451
BpuMI	CCSGG	2 cut(s)	358, 636
BsaBI	GATNNNNATC	1 cut(s)	258
BsaHI	GRCGYC	1 cut(s)	840
BsaI	GGTCTC	2 cut(s)	1392, 1442
BsaJI	CCNNGG	3 cut(s)	468, 990, 1044
BsaWI	WCCGGW	1 cut(s)	674
Bsc4I	CCNNNNNNNGG	4 cut(s)	271, 336, 634, 1494
Bse118I	RCCGGY	1 cut(s)	942
Bse1I	ACTGG	7 cut(s)	145, 455, 575, 1121, 1220, 1261, 1398
Bse3DI	GCAATG	3 cut(s)	241, 843, 1369
Bse8I	GATNNNNATC	1 cut(s)	258
BseAI	TCCGGA	1 cut(s)	674
BseBI	CCWGG	4 cut(s)	331, 629, 1045, 1384
BseDI	CCNNGG	3 cut(s)	468, 990, 1044
BseGI	GGATG	4 cut(s)	175, 717, 1179, 1252
BseJI	GATNNNNATC	1 cut(s)	258
BseLI	CCNNNNNNNGG	4 cut(s)	271, 336, 634, 1494
BseMI	GCAATG	3 cut(s)	241, 843, 1369
BseMII	CTCAG	1 cut(s)	681
BseNI	ACTGG	7 cut(s)	145, 455, 575, 1121, 1220, 1261, 1398
BseRI	GAGGAG	1 cut(s)	30
BseXI	GCAGC	3 cut(s)	933, 1086, 1346
Bsh1236I	CGCG	1 cut(s)	530
BshFI	GGCC	2 cut(s)	181, 624
BshNI	GGYRCC	2 cut(s)	631, 1091
BsiHKAI	GWGCWC	1 cut(s)	647
BsiSI	CCGG	4 cut(s)	357, 635, 675, 943
BsiWI	CGTACG	1 cut(s)	525
BslFI	GGGAC	5 cut(s)	139, 206, 315, 467, 1148
BslI	CCNNNNNNNGG	4 cut(s)	271, 336, 634, 1494
BsmAI	GTCTC	4 cut(s)	401, 729, 1392, 1442
BsmBI	CGTCTC	1 cut(s)	401
BsmFI	GGGAC	5 cut(s)	139, 206, 315, 467, 1148
BsnI	GGCC	2 cut(s)	181, 624
Bso31I	GGTCTC	2 cut(s)	1392, 1442
Bsp1286I	GDGCHC	1 cut(s)	647
Bsp13I	TCCGGA	1 cut(s)	674
Bsp1407I	TGTACA	1 cut(s)	604
Bsp143I	GATC	5 cut(s)	186, 253, 573, 779, 889
BspACI	CCGC	5 cut(s)	158, 374, 530, 659, 775
BspANI	GGCC	2 cut(s)	181, 624
BspCNI	CTCAG	1 cut(s)	682
BspEI	TCCGGA	1 cut(s)	674
BspFNI	CGCG	1 cut(s)	530
BspLI	GGNNCC	7 cut(s)	180, 355, 455, 633, 941, 988, 1093
BspMI	ACCTGC	1 cut(s)	660
BspOI	GCTAGC	1 cut(s)	1340
BspPI	GGATC	4 cut(s)	261, 581, 787, 884
BspT107I	GGYRCC	2 cut(s)	631, 1091
BspTNI	GGTCTC	2 cut(s)	1392, 1442
BsrBI	CCGCTC	1 cut(s)	661
BsrDI	GCAATG	3 cut(s)	241, 843, 1369
BsrFI	RCCGGY	1 cut(s)	942
BsrGI	TGTACA	1 cut(s)	604
BsrI	ACTGG	7 cut(s)	145, 455, 575, 1121, 1220, 1261, 1398
BssAI	RCCGGY	1 cut(s)	942
BssECI	CCNNGG	3 cut(s)	468, 990, 1044
BssMI	GATC	5 cut(s)	186, 253, 573, 779, 889
BssNI	GRCGYC	1 cut(s)	840
BssT1I	CCWWGG	2 cut(s)	468, 990
Bst2UI	CCWGG	4 cut(s)	331, 629, 1045, 1384
Bst4CI	ACNGT	3 cut(s)	266, 432, 736
Bst6I	CTCTTC	2 cut(s)	139, 688
BstACI	GRCGYC	1 cut(s)	840
BstAPI	GCANNNNNTGC	1 cut(s)	245
BstAUI	TGTACA	1 cut(s)	604
BstC8I	GCNNGC	2 cut(s)	944, 1338
BstDEI	CTNAG	2 cut(s)	690, 1286
BstF5I	GGATG	4 cut(s)	175, 717, 1179, 1252
BstFNI	CGCG	1 cut(s)	530
BstKTI	GATC	5 cut(s)	189, 256, 576, 782, 892
BstMAI	GTCTC	4 cut(s)	401, 729, 1392, 1442
BstMBI	GATC	5 cut(s)	186, 253, 573, 779, 889
BstMWI	GCNNNNNNNGC	4 cut(s)	137, 245, 413, 527
BstNI	CCWGG	4 cut(s)	331, 629, 1045, 1384
BstNSI	RCATGY	2 cut(s)	72, 540
BstSCI	CCNGG	6 cut(s)	329, 356, 627, 634, 1043, 1382
BstUI	CGCG	1 cut(s)	530
BstV1I	GCAGC	3 cut(s)	933, 1086, 1346
BstV2I	GAAGAC	2 cut(s)	480, 843
BsuI	GTATCC	1 cut(s)	1285
BsuRI	GGCC	2 cut(s)	181, 624
BtsCI	GGATG	4 cut(s)	175, 717, 1179, 1252
BtsIMutI	CAGTG	3 cut(s)	448, 568, 1227
BveI	ACCTGC	1 cut(s)	660
Cac8I	GCNNGC	2 cut(s)	944, 1338
CaiI	CAGNNNCTG	1 cut(s)	1088
Cfr10I	RCCGGY	1 cut(s)	942
Cfr13I	GGNCC	5 cut(s)	179, 454, 622, 672, 987
CseI	GACGC	2 cut(s)	644, 1289
Csp6I	GTAC	5 cut(s)	148, 526, 605, 811, 1207
CviAII	CATG	7 cut(s)	69, 537, 608, 652, 700, 1243, 1316
CviQI	GTAC	5 cut(s)	148, 526, 605, 811, 1207
DdeI	CTNAG	2 cut(s)	690, 1286
DpnI	GATC	5 cut(s)	188, 255, 575, 781, 891
DpnII	GATC	5 cut(s)	186, 253, 573, 779, 889
Eam1104I	CTCTTC	2 cut(s)	139, 688
EarI	CTCTTC	2 cut(s)	139, 688
Eco130I	CCWWGG	2 cut(s)	468, 990
Eco31I	GGTCTC	2 cut(s)	1392, 1442
Eco32I	GATATC	2 cut(s)	88, 327
Eco47I	GGWCC	3 cut(s)	454, 672, 987
EcoO109I	RGGNCCY	3 cut(s)	179, 454, 987
EcoRI	GAATTC	2 cut(s)	1141, 1513
EcoRII	CCWGG	4 cut(s)	329, 627, 1043, 1382
EcoRV	GATATC	2 cut(s)	88, 327
EcoT14I	CCWWGG	2 cut(s)	468, 990
EcoT22I	ATGCAT	1 cut(s)	409
ErhI	CCWWGG	2 cut(s)	468, 990
Esp3I	CGTCTC	1 cut(s)	401
FaeI	CATG	7 cut(s)	72, 540, 611, 655, 703, 1246, 1319
FalI	AAGNNNNNCTT	2 cut(s)	166, 198
FaqI	GGGAC	5 cut(s)	139, 206, 315, 467, 1148
FatI	CATG	7 cut(s)	68, 536, 607, 651, 699, 1242, 1315
FauI	CCCGC	2 cut(s)	367, 768
FblI	GTMKAC	2 cut(s)	971, 1063
Fnu4HI	GCNGC	3 cut(s)	947, 1075, 1360
FokI	GGATG	4 cut(s)	182, 704, 1186, 1259
Fsp4HI	GCNGC	3 cut(s)	947, 1075, 1360
FspBI	CTAG	3 cut(s)	305, 413, 1337
GluI	GCNGC	3 cut(s)	947, 1075, 1360
GsuI	CTGGAG	2 cut(s)	611, 1203
HaeIII	GGCC	2 cut(s)	181, 624
HapII	CCGG	4 cut(s)	357, 635, 675, 943
HgaI	GACGC	2 cut(s)	644, 1289
Hin1I	GRCGYC	1 cut(s)	840
Hin1II	CATG	7 cut(s)	72, 540, 611, 655, 703, 1246, 1319
HinfI	GANTC	5 cut(s)	334, 968, 1085, 1419, 1484
HpaII	CCGG	4 cut(s)	357, 635, 675, 943
HphI	GGTGA	4 cut(s)	254, 566, 574, 1222
Hpy166II	GTNNAC	3 cut(s)	972, 1064, 1500
Hpy188I	TCNGA	5 cut(s)	85, 339, 967, 1058, 1084
Hpy188III	TCNNGA	7 cut(s)	124, 675, 887, 1428, 1468, 1501, 1510
Hpy8I	GTNNAC	3 cut(s)	972, 1064, 1500
HpyAV	CCTTC	9 cut(s)	146, 168, 192, 286, 633, 924, 1033, 1112, 1474
HpyCH4III	ACNGT	3 cut(s)	266, 432, 736
HpyCH4IV	ACGT	2 cut(s)	524, 840
HpyCH4V	TGCA	8 cut(s)	239, 283, 407, 521, 669, 848, 1328, 1362
HpyF10VI	GCNNNNNNNGC	4 cut(s)	137, 245, 413, 527
HpyF3I	CTNAG	2 cut(s)	690, 1286
HpySE526I	ACGT	2 cut(s)	524, 840
Hsp92I	GRCGYC	1 cut(s)	840
Hsp92II	CATG	7 cut(s)	72, 540, 611, 655, 703, 1246, 1319
Kpn2I	TCCGGA	1 cut(s)	674
KroI	GCCGGC	1 cut(s)	942
KroNI	GCCGGC	1 cut(s)	944
Kzo9I	GATC	5 cut(s)	186, 253, 573, 779, 889
LmnI	GCTCC	1 cut(s)	939
Lsp1109I	GCAGC	3 cut(s)	933, 1086, 1346
LweI	GCATC	3 cut(s)	298, 394, 800
MaeI	CTAG	3 cut(s)	305, 413, 1337
MaeII	ACGT	2 cut(s)	524, 840
MaeIII	GTNAC	3 cut(s)	34, 436, 554
MalI	GATC	5 cut(s)	188, 255, 575, 781, 891
MbiI	CCGCTC	1 cut(s)	661
MboI	GATC	5 cut(s)	186, 253, 573, 779, 889
MboII	GAAGA	6 cut(s)	126, 287, 485, 675, 848, 939
MhlI	GDGCHC	1 cut(s)	647
MlyI	GAGTC	4 cut(s)	328, 977, 1079, 1478
MmeI	TCCRAC	1 cut(s)	89
Mph1103I	ATGCAT	1 cut(s)	409
MroI	TCCGGA	1 cut(s)	674
MroNI	GCCGGC	1 cut(s)	942
MroXI	GAANNNNTTC	1 cut(s)	682
MseI	TTAA	9 cut(s)	162, 366, 981, 1008, 1052, 1068, 1184, 1197, 1305
MslI	CAYNNNNRTG	2 cut(s)	552, 612
MspA1I	CMGCKG	1 cut(s)	775
MspI	CCGG	4 cut(s)	357, 635, 675, 943
MspR9I	CCNGG	6 cut(s)	331, 358, 629, 636, 1045, 1384
MvaI	CCWGG	4 cut(s)	331, 629, 1045, 1384
MvnI	CGCG	1 cut(s)	530
MwoI	GCNNNNNNNGC	4 cut(s)	137, 245, 413, 527
NaeI	GCCGGC	1 cut(s)	944
NciI	CCSGG	2 cut(s)	358, 636
NdeII	GATC	5 cut(s)	186, 253, 573, 779, 889
NgoMIV	GCCGGC	1 cut(s)	942
NheI	GCTAGC	1 cut(s)	1336
NlaIII	CATG	7 cut(s)	72, 540, 611, 655, 703, 1246, 1319
NlaIV	GGNNCC	7 cut(s)	180, 355, 455, 633, 941, 988, 1093
NmuCI	GTSAC	2 cut(s)	436, 554
NsiI	ATGCAT	1 cut(s)	409
NspI	RCATGY	2 cut(s)	72, 540
PciI	ACATGT	1 cut(s)	68
PdiI	GCCGGC	1 cut(s)	944
PdmI	GAANNNNTTC	1 cut(s)	682
PfeI	GAWTC	1 cut(s)	1419
Pfl23II	CGTACG	1 cut(s)	525
PfoI	TCCNGGA	1 cut(s)	329
PkrI	GCNGC	3 cut(s)	948, 1076, 1361
PleI	GAGTC	4 cut(s)	328, 976, 1079, 1478
PpsI	GAGTC	4 cut(s)	328, 976, 1079, 1478
PpuMI	RGGWCCY	2 cut(s)	454, 987
PscI	ACATGT	1 cut(s)	68
PshBI	ATTAAT	3 cut(s)	366, 1008, 1184
PsiI	TTATAA	2 cut(s)	270, 1455
Psp5II	RGGWCCY	2 cut(s)	454, 987
Psp6I	CCWGG	4 cut(s)	329, 627, 1043, 1382
PspGI	CCWGG	4 cut(s)	329, 627, 1043, 1382
PspLI	CGTACG	1 cut(s)	525
PspN4I	GGNNCC	7 cut(s)	180, 355, 455, 633, 941, 988, 1093
PspPI	GGNCC	5 cut(s)	179, 454, 622, 672, 987
PspPPI	RGGWCCY	2 cut(s)	454, 987
PstNI	CAGNNNCTG	1 cut(s)	1088
RsaI	GTAC	5 cut(s)	149, 527, 606, 812, 1208
RsaNI	GTAC	5 cut(s)	148, 526, 605, 811, 1207
RseI	CAYNNNNRTG	2 cut(s)	552, 612
SaqAI	TTAA	9 cut(s)	162, 366, 981, 1008, 1052, 1068, 1184, 1197, 1305
SatI	GCNGC	3 cut(s)	947, 1075, 1360
Sau3AI	GATC	5 cut(s)	186, 253, 573, 779, 889
Sau96I	GGNCC	5 cut(s)	179, 454, 622, 672, 987
SchI	GAGTC	4 cut(s)	328, 977, 1079, 1478
ScrFI	CCNGG	6 cut(s)	331, 358, 629, 636, 1045, 1384
SduI	GDGCHC	1 cut(s)	647
SfaNI	GCATC	3 cut(s)	298, 394, 800
SinI	GGWCC	3 cut(s)	454, 672, 987
SmiMI	CAYNNNNRTG	2 cut(s)	552, 612
SmlI	CTYRAG	3 cut(s)	462, 1274, 1466
SmoI	CTYRAG	3 cut(s)	462, 1274, 1466
SsiI	CCGC	5 cut(s)	158, 374, 530, 659, 775
SspMI	CTAG	3 cut(s)	305, 413, 1337
StyD4I	CCNGG	6 cut(s)	329, 356, 627, 634, 1043, 1382
StyI	CCWWGG	2 cut(s)	468, 990
TaaI	ACNGT	3 cut(s)	266, 432, 736
TaiI	ACGT	2 cut(s)	527, 843
TaqI	TCGA	3 cut(s)	185, 323, 1429
TatI	WGTACW	1 cut(s)	604
TfiI	GAWTC	1 cut(s)	1419
Tru1I	TTAA	9 cut(s)	162, 366, 981, 1008, 1052, 1068, 1184, 1197, 1305
Tru9I	TTAA	9 cut(s)	162, 366, 981, 1008, 1052, 1068, 1184, 1197, 1305
TscAI	CASTG	3 cut(s)	455, 575, 1227
TseFI	GTSAC	2 cut(s)	436, 554
TseI	GCWGC	3 cut(s)	946, 1074, 1359
Tsp45I	GTSAC	2 cut(s)	436, 554
TspDTI	ATGAA	3 cut(s)	305, 1202, 1255
TspGWI	ACGGA	2 cut(s)	744, 1477
TspRI	CASTG	3 cut(s)	455, 575, 1227
VpaK11BI	GGWCC	3 cut(s)	454, 672, 987
VspI	ATTAAT	3 cut(s)	366, 1008, 1184
XapI	RAATTY	5 cut(s)	866, 1141, 1330, 1504, 1513
XceI	RCATGY	2 cut(s)	72, 540
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1113
XmiI	GTMKAC	2 cut(s)	971, 1063
XmnI	GAANNNNTTC	1 cut(s)	682
XspI	CTAG	3 cut(s)	305, 413, 1337
ZraI	GACGTC	1 cut(s)	841
Zsp2I	ATGCAT	1 cut(s)	409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.