Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004007

Physical Location & Seq

Genomic Coordinates Forward (+)

5066074 .. 5069352

Gene Structure 3279 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1072100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

ATGAGTGACCACCTCGTGTTGTACGTTGACCGCCTCCTTCGGCCCATGGCAGCGCAGCCGCCTTCCGAGCCTGCTGAGGCTGGTTCTGTGCCTGAGCCGGCTGGACCGGAGCCAGAACGGGGTGCTGCCGGGCCGTCTTCGAGTTCGTCATCGGAGGAGGCGGATGAGGAGGAACCGCTGATTCAGACGGCGGAGTGCCGCATTTGCCAGGATGAGGATAGCATTAGCAATTTGGAATCTCCTTGCGCCTGTTGTGGCAGCCTCAAGTATGCTCATAGAAAATGTGTTCAGTATTGGTGCAATGAGAAAGGGGATATAACTTGTGAAATTTGTCATCAGCCTTACCAACCTGGCTACACTGCACCTCCTCGTCCACCATCTGATGAAACTACTATTGAAATTGGTGGTGGCTGGACACTTTCTGGCACTCCCTTGAGTTTGGATGATCCACGCATTTTGTCAATTGCAGAGGCAGAGCGTCAATTTATGGATACTGAATATGATGATTATTCTTCAAGTAGTAGTGGAGCTCCTTTCTTCCGTTCAGCTGCCTTAATTTTAATGGCCCTTCTAATCTTGCGGCATGCATTGAGGGCCTCGGTTTCTGACTCCGACTCTGGGGATGACAGTGACACATCTGCCATTTTCTCTCTTTTCTTGCTTAGAGCGGCTGGCTTTCTACTGCCCTGCTATATTATGGCTTGGGCCATCAGTATCTTGCAGCGTCGACAGCAAAGACAGGAGGCCGCAGCAATGGCAGCAGCCCAAGTTGCTTTTGTTCTTCAATCCGGGCAACATAGGGGTTTGCAATTTGCGGTAGCACCAGGACCCAGCGGAACTCCACATCCAGAAACTGCACATCAAGAAACTGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005768 GO:0005773 GO:0012505 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0097708

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3675 RINGv

Protein Analysis

292

Amino Acids

31.35

Weight (kDa)

4.55

Isoelectric Point (pI)

67.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RINGv	PF12906	66 - 111	7.6e-12	RING-variant domain
DUF3675	PF12428	117 - 237	2.4e-35	Protein of unknown function (DUF3675)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02960 AT2G02960 AT2G02960 AT2G02960 AT2G02960 AT2G02960
fragaria_vesca	FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340 FvH4_4g31340
malus_domestica	MD13G1072100.v1.1 MD16G1072700.v1.1
prunus_persica	Prupe.1G274900_v2.0.a1 Prupe.1G274900_v2.0.a1
pyrus_communis	pycom13g06470 pycom16g06300
rosa_chinensis	RchiOBHm_Chr4g0439841
rosa_laevigata	RLG00000006235
rosa_multiflora	Rmu_sc0027721.1_g000001
rosa_roxburghii	Rroxscaffold_5G00380740
rosa_rugosa	Rorug04G0321800 Rorug04G0321800
rosa_samantha	Rh4AG372600 Rh4BG385000 Rh4CG399400 Rh4DG379300
rosa_wichuraiana	Rw4G031850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	668
AccI	GTMKAC	1 cut(s)	727
AclWI	GGATC	1 cut(s)	440
AcsI	RAATTY	1 cut(s)	327
AdeI	CACNNNGTG	1 cut(s)	16
AfaI	GTAC	1 cut(s)	23
AfiI	CCNNNNNNNGG	3 cut(s)	119, 214, 618
AgsI	TTSAA	3 cut(s)	398, 516, 785
AjnI	CCWGG	3 cut(s)	207, 349, 823
AloI	GAACNNNNNNTCC	2 cut(s)	829, 861
AluBI	AGCT	2 cut(s)	530, 548
AluI	AGCT	2 cut(s)	530, 548
Alw21I	GWGCWC	1 cut(s)	532
AlwI	GGATC	1 cut(s)	440
AlwNI	CAGNNNCTG	1 cut(s)	854
AoxI	GGCC	6 cut(s)	41, 131, 564, 594, 705, 744
ApeKI	GCWGC	9 cut(s)	50, 55, 125, 258, 548, 721, 749, 758, 761
ApoI	RAATTY	1 cut(s)	327
AspLEI	GCGC	2 cut(s)	55, 248
AspS9I	GGNCC	7 cut(s)	42, 104, 131, 565, 594, 705, 827
AsuC2I	CCSGG	2 cut(s)	130, 790
AvaII	GGWCC	2 cut(s)	104, 827
BanII	GRGCYC	1 cut(s)	532
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	1 cut(s)	129
Bbv12I	GWGCWC	1 cut(s)	532
BbvCI	CCTCAGC	1 cut(s)	75
BbvI	GCAGC	9 cut(s)	62, 67, 112, 270, 535, 733, 761, 770, 773
BccI	CCATC	2 cut(s)	385, 716
BceAI	ACGGC	2 cut(s)	118, 204
BciT130I	CCWGG	3 cut(s)	209, 351, 825
BciVI	GTATCC	1 cut(s)	484
BcnI	CCSGG	2 cut(s)	130, 790
BfuI	GTATCC	1 cut(s)	484
Bme1390I	CCNGG	5 cut(s)	130, 209, 351, 790, 825
Bme18I	GGWCC	2 cut(s)	104, 827
BmgT120I	GGNCC	7 cut(s)	42, 104, 131, 565, 594, 705, 827
BmiI	GGNNCC	3 cut(s)	111, 174, 829
BmrFI	CCNGG	5 cut(s)	130, 209, 351, 790, 825
BpiI	GAAGAC	1 cut(s)	129
Bpu10I	CCTNAGC	2 cut(s)	75, 93
BpuEI	CTTGAG	2 cut(s)	248, 454
BpuMI	CCSGG	2 cut(s)	130, 790
BsaJI	CCNNGG	2 cut(s)	45, 597
BsaWI	WCCGGW	1 cut(s)	106
BsaXI	ACNNNNNCTCC	2 cut(s)	349, 379
Bsc4I	CCNNNNNNNGG	3 cut(s)	119, 214, 618
Bse118I	RCCGGY	1 cut(s)	97
Bse3DI	GCAATG	2 cut(s)	307, 759
BseBI	CCWGG	3 cut(s)	209, 351, 825
BseDI	CCNNGG	2 cut(s)	45, 597
BseGI	GGATG	5 cut(s)	169, 217, 448, 628, 844
BseLI	CCNNNNNNNGG	3 cut(s)	119, 214, 618
BseMI	GCAATG	2 cut(s)	307, 759
BseMII	CTCAG	2 cut(s)	66, 84
BseRI	GAGGAG	3 cut(s)	170, 182, 357
BseXI	GCAGC	9 cut(s)	62, 67, 112, 270, 535, 733, 761, 770, 773
BseYI	CCCAGC	1 cut(s)	830
BsgI	GTGCAG	2 cut(s)	345, 840
BshFI	GGCC	6 cut(s)	43, 133, 566, 596, 707, 746
BsiHKAI	GWGCWC	1 cut(s)	532
BsiSI	CCGG	4 cut(s)	98, 107, 129, 789
BslI	CCNNNNNNNGG	3 cut(s)	119, 214, 618
BsnI	GGCC	6 cut(s)	43, 133, 566, 596, 707, 746
Bsp1286I	GDGCHC	1 cut(s)	532
Bsp143I	GATC	1 cut(s)	445
Bsp19I	CCATGG	1 cut(s)	45
BspANI	GGCC	6 cut(s)	43, 133, 566, 596, 707, 746
BspCNI	CTCAG	2 cut(s)	67, 85
BspLI	GGNNCC	3 cut(s)	111, 174, 829
BspPI	GGATC	1 cut(s)	440
BsrBI	CCGCTC	1 cut(s)	668
BsrDI	GCAATG	2 cut(s)	307, 759
BsrFI	RCCGGY	1 cut(s)	97
BssAI	RCCGGY	1 cut(s)	97
BssECI	CCNNGG	2 cut(s)	45, 597
BssMI	GATC	1 cut(s)	445
BssSI	CACGAG	1 cut(s)	14
BssT1I	CCWWGG	1 cut(s)	45
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	3 cut(s)	209, 351, 825
Bst4CI	ACNGT	1 cut(s)	629
BstC8I	GCNNGC	4 cut(s)	72, 99, 585, 673
BstDEI	CTNAG	3 cut(s)	75, 93, 662
BstDSI	CCRYGG	1 cut(s)	45
BstF5I	GGATG	5 cut(s)	169, 217, 448, 628, 844
BstHHI	GCGC	2 cut(s)	55, 248
BstKTI	GATC	1 cut(s)	448
BstMBI	GATC	1 cut(s)	445
BstMWI	GCNNNNNNNGC	7 cut(s)	67, 204, 593, 730, 755, 758, 770
BstNI	CCWGG	3 cut(s)	209, 351, 825
BstNSI	RCATGY	1 cut(s)	587
BstSCI	CCNGG	5 cut(s)	128, 207, 349, 788, 823
BstV1I	GCAGC	9 cut(s)	62, 67, 112, 270, 535, 733, 761, 770, 773
BstV2I	GAAGAC	1 cut(s)	129
BsuI	GTATCC	1 cut(s)	484
BsuRI	GGCC	6 cut(s)	43, 133, 566, 596, 707, 746
BtgI	CCRYGG	1 cut(s)	45
BtsCI	GGATG	5 cut(s)	169, 217, 448, 628, 844
BtsI	GCAGTG	1 cut(s)	357
BtsIMutI	CAGTG	2 cut(s)	357, 634
Cac8I	GCNNGC	4 cut(s)	72, 99, 585, 673
CaiI	CAGNNNCTG	1 cut(s)	854
CfoI	GCGC	2 cut(s)	55, 248
Cfr10I	RCCGGY	1 cut(s)	97
Cfr13I	GGNCC	7 cut(s)	42, 104, 131, 565, 594, 705, 827
CseI	GACGC	2 cut(s)	467, 713
Csp6I	GTAC	1 cut(s)	22
CspCI	CAANNNNNGTGG	2 cut(s)	438, 473
CviAII	CATG	2 cut(s)	46, 584
CviQI	GTAC	1 cut(s)	22
DdeI	CTNAG	3 cut(s)	75, 93, 662
DpnI	GATC	1 cut(s)	447
DpnII	GATC	1 cut(s)	445
DraIII	CACNNNGTG	1 cut(s)	16
EciI	GGCGGA	2 cut(s)	176, 206
Ecl136II	GAGCTC	1 cut(s)	530
Eco130I	CCWWGG	1 cut(s)	45
Eco24I	GRGCYC	1 cut(s)	532
Eco47I	GGWCC	2 cut(s)	104, 827
Eco53kI	GAGCTC	1 cut(s)	530
EcoICRI	GAGCTC	1 cut(s)	530
EcoO109I	RGGNCCY	2 cut(s)	594, 827
EcoRII	CCWGG	3 cut(s)	207, 349, 823
EcoT14I	CCWWGG	1 cut(s)	45
EcoT22I	ATGCAT	1 cut(s)	589
EcoT38I	GRGCYC	1 cut(s)	532
ErhI	CCWWGG	1 cut(s)	45
FaeI	CATG	2 cut(s)	49, 587
FatI	CATG	2 cut(s)	45, 583
FblI	GTMKAC	1 cut(s)	727
FokI	GGATG	5 cut(s)	176, 224, 455, 635, 831
FriOI	GRGCYC	1 cut(s)	532
GlaI	GCGC	2 cut(s)	54, 247
GsaI	CCCAGC	1 cut(s)	834
HaeIII	GGCC	6 cut(s)	43, 133, 566, 596, 707, 746
HapII	CCGG	4 cut(s)	98, 107, 129, 789
HgaI	GACGC	2 cut(s)	467, 713
HhaI	GCGC	2 cut(s)	55, 248
Hin1II	CATG	2 cut(s)	49, 587
Hin6I	GCGC	2 cut(s)	53, 246
HinP1I	GCGC	2 cut(s)	53, 246
HincII	GTYRAC	2 cut(s)	28, 728
HindII	GTYRAC	2 cut(s)	28, 728
HinfI	GANTC	4 cut(s)	181, 236, 608, 614
HpaII	CCGG	4 cut(s)	98, 107, 129, 789
Hpy166II	GTNNAC	3 cut(s)	28, 374, 728
Hpy188I	TCNGA	6 cut(s)	67, 154, 186, 382, 607, 613
Hpy188III	TCNNGA	2 cut(s)	848, 863
Hpy8I	GTNNAC	3 cut(s)	28, 374, 728
Hpy99I	CGWCG	1 cut(s)	729
HpyAV	CCTTC	3 cut(s)	47, 72, 578
HpyCH4III	ACNGT	1 cut(s)	629
HpyCH4IV	ACGT	1 cut(s)	24
HpyCH4V	TGCA	7 cut(s)	300, 362, 467, 587, 721, 808, 857
HpyF10VI	GCNNNNNNNGC	7 cut(s)	67, 204, 593, 730, 755, 758, 770
HpyF3I	CTNAG	3 cut(s)	75, 93, 662
HpySE526I	ACGT	1 cut(s)	24
Hsp92II	CATG	2 cut(s)	49, 587
HspAI	GCGC	2 cut(s)	53, 246
KroI	GCCGGC	1 cut(s)	97
KroNI	GCCGGC	1 cut(s)	99
Kzo9I	GATC	1 cut(s)	445
LmnI	GCTCC	3 cut(s)	109, 527, 535
Lsp1109I	GCAGC	9 cut(s)	62, 67, 112, 270, 535, 733, 761, 770, 773
MaeII	ACGT	1 cut(s)	24
MaeIII	GTNAC	2 cut(s)	5, 629
MalI	GATC	1 cut(s)	447
MbiI	CCGCTC	1 cut(s)	668
MboI	GATC	1 cut(s)	445
MboII	GAAGA	4 cut(s)	129, 504, 529, 773
MfeI	CAATTG	1 cut(s)	462
MhlI	GDGCHC	1 cut(s)	532
MluCI	AATT	7 cut(s)	229, 327, 399, 462, 482, 555, 809
MlyI	GAGTC	2 cut(s)	602, 608
MmeI	TCCRAC	1 cut(s)	636
Mph1103I	ATGCAT	1 cut(s)	589
MroNI	GCCGGC	1 cut(s)	97
MseI	TTAA	2 cut(s)	554, 560
MspA1I	CMGCKG	3 cut(s)	178, 548, 834
MspI	CCGG	4 cut(s)	98, 107, 129, 789
MspR9I	CCNGG	5 cut(s)	130, 209, 351, 790, 825
MunI	CAATTG	1 cut(s)	462
MvaI	CCWGG	3 cut(s)	209, 351, 825
MwoI	GCNNNNNNNGC	7 cut(s)	67, 204, 593, 730, 755, 758, 770
NaeI	GCCGGC	1 cut(s)	99
NciI	CCSGG	2 cut(s)	130, 790
NcoI	CCATGG	1 cut(s)	45
NdeII	GATC	1 cut(s)	445
NgoMIV	GCCGGC	1 cut(s)	97
NlaIII	CATG	2 cut(s)	49, 587
NlaIV	GGNNCC	3 cut(s)	111, 174, 829
NmuCI	GTSAC	2 cut(s)	5, 629
NsiI	ATGCAT	1 cut(s)	589
NspI	RCATGY	1 cut(s)	587
PaeI	GCATGC	1 cut(s)	587
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PdiI	GCCGGC	1 cut(s)	99
PfeI	GAWTC	2 cut(s)	181, 236
PleI	GAGTC	2 cut(s)	602, 608
PpsI	GAGTC	2 cut(s)	602, 608
PpuMI	RGGWCCY	1 cut(s)	827
Psp124BI	GAGCTC	1 cut(s)	532
Psp5II	RGGWCCY	1 cut(s)	827
Psp6I	CCWGG	3 cut(s)	207, 349, 823
PspFI	CCCAGC	1 cut(s)	830
PspGI	CCWGG	3 cut(s)	207, 349, 823
PspN4I	GGNNCC	3 cut(s)	111, 174, 829
PspPI	GGNCC	7 cut(s)	42, 104, 131, 565, 594, 705, 827
PspPPI	RGGWCCY	1 cut(s)	827
PstNI	CAGNNNCTG	1 cut(s)	854
PvuII	CAGCTG	1 cut(s)	548
RsaI	GTAC	1 cut(s)	23
RsaNI	GTAC	1 cut(s)	22
SacI	GAGCTC	1 cut(s)	532
SalI	GTCGAC	1 cut(s)	726
SaqAI	TTAA	2 cut(s)	554, 560
Sau3AI	GATC	1 cut(s)	445
Sau96I	GGNCC	7 cut(s)	42, 104, 131, 565, 594, 705, 827
SchI	GAGTC	2 cut(s)	602, 608
ScrFI	CCNGG	5 cut(s)	130, 209, 351, 790, 825
SduI	GDGCHC	1 cut(s)	532
SetI	ASST	6 cut(s)	15, 27, 352, 367, 532, 550
SinI	GGWCC	2 cut(s)	104, 827
SmlI	CTYRAG	2 cut(s)	263, 433
SmoI	CTYRAG	2 cut(s)	263, 433
SphI	GCATGC	1 cut(s)	587
Sse9I	AATT	7 cut(s)	229, 327, 399, 462, 482, 555, 809
SstI	GAGCTC	1 cut(s)	532
StyD4I	CCNGG	5 cut(s)	128, 207, 349, 788, 823
StyI	CCWWGG	1 cut(s)	45
TaaI	ACNGT	1 cut(s)	629
TaiI	ACGT	1 cut(s)	27
TaqI	TCGA	2 cut(s)	140, 727
TasI	AATT	7 cut(s)	229, 327, 399, 462, 482, 555, 809
TauI	GCSGC	5 cut(s)	61, 201, 583, 671, 749
TfiI	GAWTC	2 cut(s)	181, 236
Tru1I	TTAA	2 cut(s)	554, 560
Tru9I	TTAA	2 cut(s)	554, 560
TscAI	CASTG	2 cut(s)	364, 634
TseFI	GTSAC	2 cut(s)	5, 629
TseI	GCWGC	9 cut(s)	50, 55, 125, 258, 548, 721, 749, 758, 761
Tsp45I	GTSAC	2 cut(s)	5, 629
TspDTI	ATGAA	1 cut(s)	399
TspGWI	ACGGA	1 cut(s)	530
TspRI	CASTG	2 cut(s)	364, 634
VpaK11BI	GGWCC	2 cut(s)	104, 827
XapI	RAATTY	1 cut(s)	327
XceI	RCATGY	1 cut(s)	587
XmiI	GTMKAC	1 cut(s)	727
Zsp2I	ATGCAT	1 cut(s)	589

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.