MD13G1078800.v1.1

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003994
Physical Location & Seq
Reverse (-)
5529464 .. 5534024
4561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1078800.v1.1.491

Sequence Viewer

Length: 861 bp
ATGAAAATGGGACAAGTTCTTGGTTGTGTTCAAGTGGACCAGTCTACTGTTGCTATCAGGGAAACTTTTGGGAAGTTCGATGATGTGCTTGAACCTGGCTGTCACTGTTTGCCATGGTGTTTGGGTAGCCAGATAGCTGGTCATCTCTCTTTACGTGTGCAGCAATTGGATGTTCGTTGTGAAACAAAGACGAAGGATAATGTTTTTGTTACTGTGGTTGCCTCTGTTCAATACCGAGCTTTGGCTGAGAAGGCTTCTGATGCTTTTTACAAGCTCAGCAATACCAGAGGGCAGATCCAGTCTTATGTCTTTGACGTTATCAGGGCAAGTGTTCCAAAGTTGGACCTAGATTCGACCTTTGAGCAGAAGAATGATATAGCCAAAGCTGTGGAAGATGAACTTGCAAAGGCCATGTCGCATTATGGGTTTGAGATAGTTCAAACACTAATTGTGGATATCGAACCAGATGAGCATGTGAAGAGAGCAATGAATGAGATAAATGCAGCTGCTAGGATGAGGCTGGCTGCAACCGAGAAAGCTGAAGCAGAGAAGATACTGCAGATTAAGCGAGCTGAGGGAGAGGCGGAGTCCAAGTACCTGTCGGGGCTCGGCATAGCAAGGCAGCGCCAGGCCATTGTGGACGGGCTGAGAGACAGTGTGCTGGCCTTCTCTGAGAACGTACCCGGGACATCATCCAAGGATGTCATGGACATGGTTCTGGTGACCCAGTACTTCGACACATTGAAGGACATCGGTGCGTCCTCAAAGTCCAACTCGGTTTTCATCCCGCACGGACCCGGTGCTGTGAAAGATATCTCTTCACAGATAAGAGACGGCCTCCTTCAAGGAAGTCGGGTGTAA

Protein Analysis

287

Amino Acids

31.41

Weight (kDa)

5.64

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 11 - 184 3e-29 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 44
AciI CCGC 2 cut(s) 584, 788
AclWI GGATC 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 3 cut(s) 596, 681, 731
AfiI CCNNNNNNNGG 1 cut(s) 241
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 6 cut(s) 32, 92, 230, 440, 745, 845
AjnI CCWGG 2 cut(s) 94, 627
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AluBI AGCT 7 cut(s) 137, 239, 274, 386, 506, 539, 572
AluI AGCT 7 cut(s) 137, 239, 274, 386, 506, 539, 572
Alw26I GTCTC 2 cut(s) 645, 825
AlwI GGATC 1 cut(s) 289
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 4 cut(s) 408, 630, 663, 835
ApeKI GCWGC 5 cut(s) 160, 503, 506, 524, 622
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 3 cut(s) 37, 343, 794
AsuC2I CCSGG 3 cut(s) 684, 685, 798
AsuHPI GGTGA 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 3 cut(s) 37, 343, 794
BanII GRGCYC 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 5 cut(s) 172, 493, 511, 515, 634
BceAI ACGGC 1 cut(s) 850
BciT130I CCWGG 2 cut(s) 96, 629
BcnI CCSGG 3 cut(s) 684, 685, 798
BcoDI GTCTC 2 cut(s) 645, 825
BfaI CTAG 2 cut(s) 347, 510
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 1 cut(s) 628
BisI GCNGC 5 cut(s) 161, 504, 507, 525, 623
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 5 cut(s) 162, 505, 508, 526, 624
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 5 cut(s) 96, 629, 684, 685, 798
Bme18I GGWCC 3 cut(s) 37, 343, 794
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 3 cut(s) 37, 343, 794
BmiI GGNNCC 1 cut(s) 796
BmrFI CCNGG 5 cut(s) 96, 629, 684, 685, 798
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 1 cut(s) 250
BmuI ACTGGG 1 cut(s) 721
BplI GAGNNNNNCTC 2 cut(s) 822, 854
Bpu10I CCTNAGC 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 275
BpuMI CCSGG 3 cut(s) 684, 685, 798
BsaAI YACGTR 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 113, 683, 696
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 3 cut(s) 40, 298, 727
Bse3DI GCAATG 1 cut(s) 492
BseBI CCWGG 2 cut(s) 96, 629
BseDI CCNNGG 3 cut(s) 113, 683, 696
BseGI GGATG 5 cut(s) 175, 519, 692, 706, 783
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 5 cut(s) 237, 289, 564, 638, 663
BseNI ACTGG 3 cut(s) 40, 298, 727
BseXI GCAGC 5 cut(s) 172, 493, 511, 515, 634
BsgI GTGCAG 1 cut(s) 179
BshFI GGCC 4 cut(s) 410, 632, 665, 837
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 2 cut(s) 684, 798
BslFI GGGAC 2 cut(s) 24, 700
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 2 cut(s) 645, 825
BsmBI CGTCTC 1 cut(s) 825
BsmFI GGGAC 2 cut(s) 24, 700
BsnI GGCC 4 cut(s) 410, 632, 665, 837
BsoBI CYCGRG 1 cut(s) 683
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 1 cut(s) 294
Bsp1720I GCTNAGC 1 cut(s) 275
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 2 cut(s) 584, 788
BspANI GGCC 4 cut(s) 410, 632, 665, 837
BspCNI CTCAG 5 cut(s) 238, 288, 565, 639, 664
BspLI GGNNCC 1 cut(s) 796
BspMAI CTGCAG 1 cut(s) 561
BspPI GGATC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 3 cut(s) 40, 298, 727
BssECI CCNNGG 3 cut(s) 113, 683, 696
BssMI GATC 1 cut(s) 294
BssT1I CCWWGG 2 cut(s) 113, 696
Bst2UI CCWGG 2 cut(s) 96, 629
Bst4CI ACNGT 4 cut(s) 49, 107, 214, 656
Bst6I CTCTTC 2 cut(s) 473, 823
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 522, 570, 663
BstDEI CTNAG 5 cut(s) 246, 275, 573, 647, 672
BstDSI CCRYGG 1 cut(s) 113
BstEII GGTNACC 1 cut(s) 721
BstF5I GGATG 5 cut(s) 175, 519, 692, 706, 783
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 2 cut(s) 645, 825
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 3 cut(s) 251, 260, 565
BstNI CCWGG 2 cut(s) 96, 629
BstNSI RCATGY 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 721
BstSCI CCNGG 5 cut(s) 94, 627, 682, 683, 796
BstSFI CTRYAG 1 cut(s) 557
BstV1I GCAGC 5 cut(s) 172, 493, 511, 515, 634
BstX2I RGATCY 1 cut(s) 294
BstXI CCANNNNNNTGG 2 cut(s) 137, 388
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 4 cut(s) 410, 632, 665, 837
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 5 cut(s) 175, 519, 692, 706, 783
BtsIMutI CAGTG 2 cut(s) 103, 661
Cac8I GCNNGC 3 cut(s) 522, 570, 663
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 3 cut(s) 37, 343, 794
Cfr9I CCCGGG 1 cut(s) 683
CseI GACGC 1 cut(s) 747
Csp6I GTAC 3 cut(s) 595, 680, 730
CviAII CATG 5 cut(s) 114, 412, 473, 706, 712
CviQI GTAC 3 cut(s) 595, 680, 730
DdeI CTNAG 5 cut(s) 246, 275, 573, 647, 672
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 473, 823
EarI CTCTTC 2 cut(s) 473, 823
EciI GGCGGA 1 cut(s) 599
Eco130I CCWWGG 2 cut(s) 113, 696
Eco24I GRGCYC 1 cut(s) 609
Eco32I GATATC 2 cut(s) 457, 814
Eco47I GGWCC 3 cut(s) 37, 343, 794
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 683
Eco91I GGTNACC 1 cut(s) 721
EcoO65I GGTNACC 1 cut(s) 721
EcoRII CCWGG 2 cut(s) 94, 627
EcoRV GATATC 2 cut(s) 457, 814
EcoT14I CCWWGG 2 cut(s) 113, 696
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 2 cut(s) 113, 696
Esp3I CGTCTC 1 cut(s) 825
FaeI CATG 5 cut(s) 117, 415, 476, 709, 715
FaiI YATR 9 cut(s) 115, 306, 377, 413, 423, 474, 614, 707, 713
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 2 cut(s) 24, 700
FatI CATG 5 cut(s) 113, 411, 472, 705, 711
FauI CCCGC 1 cut(s) 795
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 5 cut(s) 161, 504, 507, 525, 623
FokI GGATG 5 cut(s) 182, 526, 679, 713, 770
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 5 cut(s) 161, 504, 507, 525, 623
FspBI CTAG 2 cut(s) 347, 510
GlaI GCGC 1 cut(s) 626
GluI GCNGC 5 cut(s) 161, 504, 507, 525, 623
HaeII RGCGCY 1 cut(s) 628
HaeIII GGCC 4 cut(s) 410, 632, 665, 837
HapII CCGG 2 cut(s) 684, 798
HgaI GACGC 1 cut(s) 747
HhaI GCGC 1 cut(s) 627
Hin1II CATG 5 cut(s) 117, 415, 476, 709, 715
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HinfI GANTC 2 cut(s) 350, 587
HpaII CCGG 2 cut(s) 684, 798
HphI GGTGA 1 cut(s) 733
Hpy166II GTNNAC 3 cut(s) 37, 45, 640
Hpy188I TCNGA 2 cut(s) 259, 673
Hpy8I GTNNAC 3 cut(s) 37, 45, 640
HpyAV CCTTC 5 cut(s) 187, 244, 676, 739, 851
HpyCH4III ACNGT 4 cut(s) 49, 107, 214, 656
HpyCH4IV ACGT 3 cut(s) 154, 315, 678
HpyCH4V TGCA 5 cut(s) 160, 404, 503, 527, 559
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 260, 565
HpyF3I CTNAG 5 cut(s) 246, 275, 573, 647, 672
HpySE526I ACGT 3 cut(s) 154, 315, 678
Hsp92II CATG 5 cut(s) 117, 415, 476, 709, 715
HspAI GCGC 1 cut(s) 625
Kzo9I GATC 1 cut(s) 294
Lsp1109I GCAGC 5 cut(s) 172, 493, 511, 515, 634
LweI GCATC 1 cut(s) 250
MaeI CTAG 2 cut(s) 347, 510
MaeII ACGT 3 cut(s) 154, 315, 678
MaeIII GTNAC 3 cut(s) 101, 208, 721
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 5 cut(s) 379, 404, 490, 562, 810
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 2 cut(s) 164, 447
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 2 cut(s) 321, 795
MnlI CCTC 7 cut(s) 232, 281, 510, 568, 574, 772, 848
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 710
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 2 cut(s) 684, 798
MspR9I CCNGG 5 cut(s) 96, 629, 684, 685, 798
MunI CAATTG 1 cut(s) 164
MvaI CCWGG 2 cut(s) 96, 629
MwoI GCNNNNNNNGC 3 cut(s) 251, 260, 565
NciI CCSGG 3 cut(s) 684, 685, 798
NcoI CCATGG 1 cut(s) 113
NdeII GATC 1 cut(s) 294
NlaIII CATG 5 cut(s) 117, 415, 476, 709, 715
NlaIV GGNNCC 1 cut(s) 796
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 2 cut(s) 101, 721
NspI RCATGY 1 cut(s) 476
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 5 cut(s) 162, 505, 508, 526, 624
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
Ppu21I YACGTR 1 cut(s) 155
Psp6I CCWGG 2 cut(s) 94, 627
PspEI GGTNACC 1 cut(s) 721
PspGI CCWGG 2 cut(s) 94, 627
PspN4I GGNNCC 1 cut(s) 796
PspPI GGNCC 3 cut(s) 37, 343, 794
PstI CTGCAG 1 cut(s) 561
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 3 cut(s) 596, 681, 731
RsaNI GTAC 3 cut(s) 595, 680, 730
RseI CAYNNNNRTG 1 cut(s) 710
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 5 cut(s) 161, 504, 507, 525, 623
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 3 cut(s) 37, 343, 794
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 5 cut(s) 96, 629, 684, 685, 798
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 557
SinI GGWCC 3 cut(s) 37, 343, 794
SmaI CCCGGG 1 cut(s) 685
SmiMI CAYNNNNRTG 1 cut(s) 710
Sse9I AATT 2 cut(s) 164, 447
SsiI CCGC 2 cut(s) 584, 788
SspMI CTAG 2 cut(s) 347, 510
StyD4I CCNGG 5 cut(s) 94, 627, 682, 683, 796
StyI CCWWGG 2 cut(s) 113, 696
TaaI ACNGT 4 cut(s) 49, 107, 214, 656
TaiI ACGT 3 cut(s) 157, 318, 681
TaqI TCGA 4 cut(s) 78, 353, 459, 735
TasI AATT 2 cut(s) 164, 447
TatI WGTACW 1 cut(s) 729
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TscAI CASTG 2 cut(s) 110, 661
TseFI GTSAC 2 cut(s) 101, 721
TseI GCWGC 5 cut(s) 160, 503, 506, 524, 622
Tsp45I GTSAC 2 cut(s) 101, 721
TspDTI ATGAA 4 cut(s) 17, 411, 503, 772
TspGWI ACGGA 1 cut(s) 807
TspMI CCCGGG 1 cut(s) 683
TspRI CASTG 2 cut(s) 110, 661
VpaK11BI GGWCC 3 cut(s) 37, 343, 794
XceI RCATGY 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmaI CCCGGG 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 44
XspI CTAG 2 cut(s) 347, 510
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.