MD16G1078400.v1.1

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003994
Physical Location & Seq
Reverse (-)
5502811 .. 5507716
4906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1078400.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGGACAAGCTCTTGGTTGTATTCAAGTGGACCAGTCCACTGTTGCTATCAGGGAAACTTTTGGGAAGTTTGATGATGTGCTTGCACCTGGATGTCACTGTTTGCCTTGGTGTTTTGGTAGCCAGGTAGCTGGTCGTCTCTCCTTACGTGTGCAGCAATTGGACGTTCGTTGTGAAACAAAGACAAAGGATAATGTTTTTGTTACTGTGGTTGCTTCAATTCAATACCGAGCTTTGGCTGAGAAGGCTTCTGATGCCTTTTACAAGCTCAGCAATACCAGAGGGCAGATCCAATCTTATGTCTTTGATGTGATTAGGGCAAGTGTTCCGAAATTGGACCTAGATTCAACCTTTGAGCAGAAGAATGATATAGCCAAATCTGTGGAAGAAGAACTTGAAAAGGCCATGTCACATTATGGGTTTGAGATAGTTCAAACACTAATTGTGGATATCGAACCAGATGAGCACGTGAAGAGATCGATGAATGAGATTAATGCAGCTGCTAGGATGAGGGTGGCCGCAAGTGAGAAAGCTGAAGCAGAGAAGATACTACAGATTAAGCGAGCTGAGGGAGAGGCAGAGTCCAAATATCTGTCGGGGCTTGGCATAGCAAGGCAGCGCCAGGCCATTGTGGACGGGCTGAGAGACAGCGTGCTGGCCTTCTCTGAGAACGTACCTGGGACATCATCCAAGGATGTCATGGACATGGTTCTCGTGACCCAGTACTTTGACACAATGAAGGAGATTGGTGCATCCTCAAAGTCCAATTCGGTTTTCATCCCACACGGACCAGGTGCTGTGAGAGACATTGCGTCGCAGATAAGAGACGGACTCCTTCAAGGAAGTTCGATTCAGGAGTAA

Protein Analysis

287

Amino Acids

31.46

Weight (kDa)

5.31

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 9 - 182 1.1e-28 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 519
AclWI GGATC 1 cut(s) 283
AcoI YGGCCR 1 cut(s) 516
AcuI CTGAAG 1 cut(s) 555
AcvI CACGTG 1 cut(s) 469
AfaI GTAC 2 cut(s) 675, 725
AfiI CCNNNNNNNGG 1 cut(s) 235
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 7 cut(s) 26, 219, 224, 348, 398, 434, 839
AhdI GACNNNNNGTC 1 cut(s) 811
AjnI CCWGG 5 cut(s) 88, 123, 621, 676, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 758, 790
AluBI AGCT 7 cut(s) 11, 131, 233, 268, 500, 533, 566
AluI AGCT 7 cut(s) 11, 131, 233, 268, 500, 533, 566
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 4 cut(s) 143, 639, 798, 819
AlwI GGATC 1 cut(s) 283
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 4 cut(s) 402, 516, 624, 657
ApeKI GCWGC 4 cut(s) 154, 497, 500, 616
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 1 cut(s) 621
AspS9I GGNCC 3 cut(s) 31, 337, 788
AvaII GGWCC 3 cut(s) 31, 337, 788
BauI CACGAG 1 cut(s) 713
BbrPI CACGTG 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 468
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 4 cut(s) 166, 487, 509, 628
BciT130I CCWGG 5 cut(s) 90, 125, 623, 678, 792
BcoDI GTCTC 4 cut(s) 143, 639, 798, 819
BfaI CTAG 2 cut(s) 341, 504
BfmI CTRYAG 1 cut(s) 551
BfoI RGCGCY 1 cut(s) 622
BisI GCNGC 5 cut(s) 155, 498, 501, 519, 617
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 5 cut(s) 156, 499, 502, 520, 618
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 5 cut(s) 90, 125, 623, 678, 792
Bme18I GGWCC 3 cut(s) 31, 337, 788
BmeRI GACNNNNNGTC 1 cut(s) 811
BmgT120I GGNCC 3 cut(s) 31, 337, 788
BmrFI CCNGG 5 cut(s) 90, 125, 623, 678, 792
BmrI ACTGGG 1 cut(s) 715
BmsI GCATC 2 cut(s) 244, 761
BmuI ACTGGG 1 cut(s) 715
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 479
BsaAI YACGTR 2 cut(s) 149, 469
BsaJI CCNNGG 3 cut(s) 107, 677, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 34, 721
Bse3DI GCAATG 1 cut(s) 807
BseBI CCWGG 5 cut(s) 90, 125, 623, 678, 792
BseCI ATCGAT 1 cut(s) 479
BseDI CCNNGG 3 cut(s) 107, 677, 690
BseGI GGATG 6 cut(s) 98, 513, 686, 700, 752, 777
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 5 cut(s) 231, 283, 558, 632, 657
BseNI ACTGG 2 cut(s) 34, 721
BseXI GCAGC 4 cut(s) 166, 487, 509, 628
BsgI GTGCAG 1 cut(s) 173
BshFI GGCC 4 cut(s) 404, 518, 626, 659
BshVI ATCGAT 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 468
BslFI GGGAC 2 cut(s) 18, 694
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 4 cut(s) 143, 639, 798, 819
BsmBI CGTCTC 2 cut(s) 143, 819
BsmFI GGGAC 2 cut(s) 18, 694
BsnI GGCC 4 cut(s) 404, 518, 626, 659
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 2 cut(s) 288, 476
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 1 cut(s) 519
BspANI GGCC 4 cut(s) 404, 518, 626, 659
BspCNI CTCAG 5 cut(s) 232, 282, 559, 633, 658
BspDI ATCGAT 1 cut(s) 479
BspPI GGATC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 807
BsrI ACTGG 2 cut(s) 34, 721
BssECI CCNNGG 3 cut(s) 107, 677, 690
BssMI GATC 2 cut(s) 288, 476
BssSI CACGAG 1 cut(s) 713
BssT1I CCWWGG 2 cut(s) 107, 690
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 5 cut(s) 90, 125, 623, 678, 792
Bst4CI ACNGT 3 cut(s) 43, 101, 208
Bst6I CTCTTC 1 cut(s) 467
BstBAI YACGTR 2 cut(s) 149, 469
BstC8I GCNNGC 4 cut(s) 84, 564, 653, 657
BstDEI CTNAG 5 cut(s) 240, 269, 567, 641, 666
BstF5I GGATG 6 cut(s) 98, 513, 686, 700, 752, 777
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 1 cut(s) 621
BstKTI GATC 2 cut(s) 291, 479
BstMAI GTCTC 4 cut(s) 143, 639, 798, 819
BstMBI GATC 2 cut(s) 288, 476
BstMWI GCNNNNNNNGC 2 cut(s) 245, 254
BstNI CCWGG 5 cut(s) 90, 125, 623, 678, 792
BstSCI CCNGG 5 cut(s) 88, 123, 621, 676, 790
BstSFI CTRYAG 1 cut(s) 551
BstV1I GCAGC 4 cut(s) 166, 487, 509, 628
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 2 cut(s) 131, 382
BstYI RGATCY 1 cut(s) 288
Bsu15I ATCGAT 1 cut(s) 479
BsuRI GGCC 4 cut(s) 404, 518, 626, 659
BsuTUI ATCGAT 1 cut(s) 479
BtsCI GGATG 6 cut(s) 98, 513, 686, 700, 752, 777
BtsIMutI CAGTG 2 cut(s) 39, 97
Cac8I GCNNGC 4 cut(s) 84, 564, 653, 657
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 1 cut(s) 621
Cfr13I GGNCC 3 cut(s) 31, 337, 788
ClaI ATCGAT 1 cut(s) 479
CseI GACGC 1 cut(s) 801
CsiI ACCWGGT 1 cut(s) 790
Csp6I GTAC 2 cut(s) 674, 724
CviAII CATG 3 cut(s) 406, 700, 706
CviQI GTAC 2 cut(s) 674, 724
DdeI CTNAG 5 cut(s) 240, 269, 567, 641, 666
DpnI GATC 2 cut(s) 290, 478
DpnII GATC 2 cut(s) 288, 476
DriI GACNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 467
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 467
Eco130I CCWWGG 2 cut(s) 107, 690
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 3 cut(s) 31, 337, 788
Eco57I CTGAAG 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 469
EcoRII CCWGG 5 cut(s) 88, 123, 621, 676, 790
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 107, 690
ErhI CCWWGG 2 cut(s) 107, 690
Esp3I CGTCTC 2 cut(s) 143, 819
FaeI CATG 3 cut(s) 409, 703, 709
FaiI YATR 7 cut(s) 300, 371, 407, 417, 608, 701, 707
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 18, 694
FatI CATG 3 cut(s) 405, 699, 705
Fnu4HI GCNGC 5 cut(s) 155, 498, 501, 519, 617
FokI GGATG 6 cut(s) 105, 520, 673, 707, 739, 764
Fsp4HI GCNGC 5 cut(s) 155, 498, 501, 519, 617
FspBI CTAG 2 cut(s) 341, 504
GlaI GCGC 1 cut(s) 620
GluI GCNGC 5 cut(s) 155, 498, 501, 519, 617
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 4 cut(s) 404, 518, 626, 659
HgaI GACGC 1 cut(s) 801
HhaI GCGC 1 cut(s) 621
Hin1II CATG 3 cut(s) 409, 703, 709
Hin6I GCGC 1 cut(s) 619
HinP1I GCGC 1 cut(s) 619
HinfI GANTC 4 cut(s) 344, 581, 831, 850
Hpy166II GTNNAC 3 cut(s) 31, 39, 634
Hpy188I TCNGA 3 cut(s) 253, 330, 667
Hpy188III TCNNGA 2 cut(s) 715, 854
Hpy8I GTNNAC 3 cut(s) 31, 39, 634
Hpy99I CGWCG 1 cut(s) 817
HpyAV CCTTC 4 cut(s) 238, 670, 733, 845
HpyCH4III ACNGT 3 cut(s) 43, 101, 208
HpyCH4IV ACGT 4 cut(s) 148, 165, 468, 672
HpyCH4V TGCA 4 cut(s) 86, 154, 497, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 254
HpyF3I CTNAG 5 cut(s) 240, 269, 567, 641, 666
HpySE526I ACGT 4 cut(s) 148, 165, 468, 672
Hsp92II CATG 3 cut(s) 409, 703, 709
HspAI GCGC 1 cut(s) 619
Kzo9I GATC 2 cut(s) 288, 476
Lsp1109I GCAGC 4 cut(s) 166, 487, 509, 628
LweI GCATC 2 cut(s) 244, 761
MabI ACCWGGT 1 cut(s) 790
MaeI CTAG 2 cut(s) 341, 504
MaeII ACGT 4 cut(s) 148, 165, 468, 672
MaeIII GTNAC 4 cut(s) 95, 202, 408, 715
MalI GATC 2 cut(s) 290, 478
MboI GATC 2 cut(s) 288, 476
MboII GAAGA 5 cut(s) 373, 398, 401, 484, 556
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 5 cut(s) 158, 219, 332, 441, 766
MlyI GAGTC 2 cut(s) 590, 825
MnlI CCTC 5 cut(s) 275, 504, 562, 568, 766
MseI TTAA 2 cut(s) 492, 558
MslI CAYNNNNRTG 2 cut(s) 91, 704
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 5 cut(s) 90, 125, 623, 678, 792
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 5 cut(s) 90, 125, 623, 678, 792
MwoI GCNNNNNNNGC 2 cut(s) 245, 254
NdeII GATC 2 cut(s) 288, 476
NlaIII CATG 3 cut(s) 409, 703, 709
NmuCI GTSAC 3 cut(s) 95, 408, 715
PfeI GAWTC 2 cut(s) 344, 850
PkrI GCNGC 5 cut(s) 156, 499, 502, 520, 618
PleI GAGTC 2 cut(s) 589, 825
PmaCI CACGTG 1 cut(s) 469
PmlI CACGTG 1 cut(s) 469
PpsI GAGTC 2 cut(s) 589, 825
Ppu21I YACGTR 2 cut(s) 149, 469
PshBI ATTAAT 1 cut(s) 492
Psp6I CCWGG 5 cut(s) 88, 123, 621, 676, 790
PspCI CACGTG 1 cut(s) 469
PspGI CCWGG 5 cut(s) 88, 123, 621, 676, 790
PspPI GGNCC 3 cut(s) 31, 337, 788
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 675, 725
RsaNI GTAC 2 cut(s) 674, 724
RseI CAYNNNNRTG 2 cut(s) 91, 704
SaqAI TTAA 2 cut(s) 492, 558
SatI GCNGC 5 cut(s) 155, 498, 501, 519, 617
Sau3AI GATC 2 cut(s) 288, 476
Sau96I GGNCC 3 cut(s) 31, 337, 788
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 2 cut(s) 590, 825
ScrFI CCNGG 5 cut(s) 90, 125, 623, 678, 792
SduI GDGCHC 1 cut(s) 468
SexAI ACCWGGT 1 cut(s) 790
SfaNI GCATC 2 cut(s) 244, 761
SfcI CTRYAG 1 cut(s) 551
SinI GGWCC 3 cut(s) 31, 337, 788
SmiMI CAYNNNNRTG 2 cut(s) 91, 704
Sse9I AATT 5 cut(s) 158, 219, 332, 441, 766
SsiI CCGC 1 cut(s) 519
SspMI CTAG 2 cut(s) 341, 504
StyD4I CCNGG 5 cut(s) 88, 123, 621, 676, 790
StyI CCWWGG 2 cut(s) 107, 690
TaaI ACNGT 3 cut(s) 43, 101, 208
TaiI ACGT 4 cut(s) 151, 168, 471, 675
TaqI TCGA 3 cut(s) 453, 479, 848
TasI AATT 5 cut(s) 158, 219, 332, 441, 766
TatI WGTACW 1 cut(s) 723
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 2 cut(s) 344, 850
Tru1I TTAA 2 cut(s) 492, 558
Tru9I TTAA 2 cut(s) 492, 558
TscAI CASTG 2 cut(s) 46, 104
TseFI GTSAC 3 cut(s) 95, 408, 715
TseI GCWGC 4 cut(s) 154, 497, 500, 616
Tsp45I GTSAC 3 cut(s) 95, 408, 715
TspDTI ATGAA 3 cut(s) 497, 752, 766
TspGWI ACGGA 2 cut(s) 801, 843
TspRI CASTG 2 cut(s) 46, 104
VpaK11BI GGWCC 3 cut(s) 31, 337, 788
VspI ATTAAT 1 cut(s) 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 697
XspI CTAG 2 cut(s) 341, 504
ZrmI AGTACT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.