RLG00000006166

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003994
Physical Location & Seq
Forward (+)
4875923 .. 4880053
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006166

Sequence Viewer

Length: 873 bp
ATGCTATTTCACACGAAAGTTACCGTGGAACAACTTAACCGCCACGTCCTTCTGCGAATCTTCTCCGTCACTTCCTCGCTCTCTTGCTTTTTTTTTTCTCTAACAGAATCAGAAAATGGGACAGTGCCTTGGTTCGCAGTAGCTGGTCATCTCTCTCTACGAGTGCAGCAATTGGATGTTCGCTGTGAAACAAAGACCAAGGATAACGTTTTTGTTACTGTGGTTGCTTCTATTCAATACCGAGCTTTAGCTGACAAAGCTTCAGATGCCTTCTACAAGCTCAGCAATACCAGGGGGCAGATCCAGTCTTATGTCTTTGATGTTATTAGGGCAAGTGTTCCAAAGTTGGATCTAGACTCAACCTTTGAACAGAAGAATGATATCGCCAAAGCTGTGGAAGAAGAACTTGAAAAGGCCATGTCACATTATGGGTTTGAGATAGTACAAACATTGATTGTGGATATCGAACCAGATGAGCATGTGAAGAGAGCGATGAATGAGATTAATGCAGCTGCTAGGATGAGGTTGGCTGCATCTGAGAGAGCTGAAGGAGATAAGATACTACAGATTAAGCGAGCTGAGGGAGAGGCAGAGTCCAAATATCTGTCAGGGCTTGGCATAGCAAGGCAGCGCCAGGCTATTGTGGACGGGCTGAGAGACAGCGTGCTGGCTTTCTCTGAGAATGTACCTGGGACATCATCAAAGGATGTCATGGATATGGTTCTGGTGACCCAGTACTTCGATACAATGAAAGAGATCGGGGCATCTTCAAAGTCCAATTCGGTTTTCATCCCACACGGACCTGGTGCTGTGAAAGACATTGCTTCACAGATAAGAGATGGTCTCCTTCAAGGAAATTCAACTCACCAGTGA

Protein Analysis

291

Amino Acids

32.19

Weight (kDa)

5.85

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 41 - 186 4.3e-21 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 40
AclI AACGTT 1 cut(s) 207
AclWI GGATC 2 cut(s) 295, 357
AcsI RAATTY 1 cut(s) 856
AcuI CTGAAG 2 cut(s) 246, 567
AfaI GTAC 3 cut(s) 444, 687, 737
AgsI TTSAA 6 cut(s) 236, 368, 410, 771, 851, 861
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 4 cut(s) 290, 633, 688, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 9 cut(s) 143, 245, 251, 260, 280, 392, 512, 545, 578
AluI AGCT 9 cut(s) 143, 245, 251, 260, 280, 392, 512, 545, 578
Alw26I GTCTC 2 cut(s) 651, 848
AlwI GGATC 2 cut(s) 295, 357
AlwNI CAGNNNCTG 1 cut(s) 143
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 5 cut(s) 166, 509, 512, 530, 628
ApoI RAATTY 1 cut(s) 856
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AseI ATTAAT 1 cut(s) 504
AspLEI GCGC 1 cut(s) 633
AspS9I GGNCC 1 cut(s) 800
AsuHPI GGTGA 2 cut(s) 739, 857
AvaII GGWCC 1 cut(s) 800
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 5 cut(s) 178, 499, 517, 521, 640
BccI CCATC 1 cut(s) 833
BciT130I CCWGG 4 cut(s) 292, 635, 690, 804
BcoDI GTCTC 2 cut(s) 651, 848
BfaI CTAG 2 cut(s) 353, 516
BfmI CTRYAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 634
BisI GCNGC 5 cut(s) 167, 510, 513, 531, 629
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 5 cut(s) 168, 511, 514, 532, 630
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 4 cut(s) 292, 635, 690, 804
Bme18I GGWCC 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 800
BmrFI CCNGG 4 cut(s) 292, 635, 690, 804
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 3 cut(s) 256, 542, 773
BmuI ACTGGG 1 cut(s) 727
BplI GAGNNNNNCTC 2 cut(s) 828, 860
Bpu10I CCTNAGC 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 5 cut(s) 24, 128, 198, 291, 689
Bse1I ACTGG 3 cut(s) 304, 733, 868
Bse3DI GCAATG 1 cut(s) 819
BseBI CCWGG 4 cut(s) 292, 635, 690, 804
BseDI CCNNGG 5 cut(s) 24, 128, 198, 291, 689
BseGI GGATG 4 cut(s) 181, 525, 712, 789
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 5 cut(s) 295, 528, 570, 644, 669
BseNI ACTGG 3 cut(s) 304, 733, 868
BseXI GCAGC 5 cut(s) 178, 499, 517, 521, 640
BsgI GTGCAG 1 cut(s) 185
BshFI GGCC 1 cut(s) 416
BslFI GGGAC 2 cut(s) 133, 706
BsmAI GTCTC 2 cut(s) 651, 848
BsmFI GGGAC 2 cut(s) 133, 706
BsnI GGCC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 848
Bsp143I GATC 3 cut(s) 300, 349, 756
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 1 cut(s) 40
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 5 cut(s) 294, 529, 571, 645, 670
BspPI GGATC 2 cut(s) 295, 357
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 3 cut(s) 304, 733, 868
BssECI CCNNGG 5 cut(s) 24, 128, 198, 291, 689
BssMI GATC 3 cut(s) 300, 349, 756
BssT1I CCWWGG 2 cut(s) 128, 198
Bst2UI CCWGG 4 cut(s) 292, 635, 690, 804
Bst4CI ACNGT 3 cut(s) 25, 124, 220
Bst6I CTCTTC 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 576, 665, 669
BstDEI CTNAG 5 cut(s) 281, 537, 579, 653, 678
BstDSI CCRYGG 1 cut(s) 24
BstEII GGTNACC 1 cut(s) 727
BstF5I GGATG 4 cut(s) 181, 525, 712, 789
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 3 cut(s) 303, 352, 759
BstMAI GTCTC 2 cut(s) 651, 848
BstMBI GATC 3 cut(s) 300, 349, 756
BstMWI GCNNNNNNNGC 2 cut(s) 257, 266
BstNI CCWGG 4 cut(s) 292, 635, 690, 804
BstNSI RCATGY 1 cut(s) 482
BstPI GGTNACC 1 cut(s) 727
BstSCI CCNGG 4 cut(s) 290, 633, 688, 802
BstSFI CTRYAG 1 cut(s) 563
BstV1I GCAGC 5 cut(s) 178, 499, 517, 521, 640
BstX2I RGATCY 2 cut(s) 300, 349
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 2 cut(s) 300, 349
BsuRI GGCC 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 506
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 4 cut(s) 181, 525, 712, 789
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 3 cut(s) 576, 665, 669
CaiI CAGNNNCTG 1 cut(s) 143
CfoI GCGC 1 cut(s) 633
Cfr13I GGNCC 1 cut(s) 800
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 3 cut(s) 443, 686, 736
CviAII CATG 3 cut(s) 418, 479, 712
CviQI GTAC 3 cut(s) 443, 686, 736
DdeI CTNAG 5 cut(s) 281, 537, 579, 653, 678
DpnI GATC 3 cut(s) 302, 351, 758
DpnII GATC 3 cut(s) 300, 349, 756
Eam1104I CTCTTC 1 cut(s) 479
EarI CTCTTC 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 128, 198
Eco31I GGTCTC 1 cut(s) 848
Eco32I GATATC 2 cut(s) 382, 463
Eco47I GGWCC 1 cut(s) 800
Eco57I CTGAAG 2 cut(s) 246, 567
Eco91I GGTNACC 1 cut(s) 727
EcoO65I GGTNACC 1 cut(s) 727
EcoRII CCWGG 4 cut(s) 290, 633, 688, 802
EcoRV GATATC 2 cut(s) 382, 463
EcoT14I CCWWGG 2 cut(s) 128, 198
ErhI CCWWGG 2 cut(s) 128, 198
FaeI CATG 3 cut(s) 421, 482, 715
FaiI YATR 7 cut(s) 312, 419, 429, 480, 620, 713, 719
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 2 cut(s) 133, 706
FatI CATG 3 cut(s) 417, 478, 711
Fnu4HI GCNGC 5 cut(s) 167, 510, 513, 531, 629
FokI GGATG 4 cut(s) 188, 532, 719, 776
Fsp4HI GCNGC 5 cut(s) 167, 510, 513, 531, 629
FspBI CTAG 2 cut(s) 353, 516
GlaI GCGC 1 cut(s) 632
GluI GCNGC 5 cut(s) 167, 510, 513, 531, 629
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 1 cut(s) 416
HhaI GCGC 1 cut(s) 633
Hin1II CATG 3 cut(s) 421, 482, 715
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 4 cut(s) 57, 107, 356, 593
HphI GGTGA 2 cut(s) 739, 857
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 4 cut(s) 112, 265, 538, 679
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 646
HpyAV CCTTC 4 cut(s) 59, 280, 542, 857
HpyCH4III ACNGT 3 cut(s) 25, 124, 220
HpyCH4IV ACGT 2 cut(s) 45, 207
HpyCH4V TGCA 3 cut(s) 166, 509, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 266
HpyF3I CTNAG 5 cut(s) 281, 537, 579, 653, 678
HpySE526I ACGT 2 cut(s) 45, 207
Hsp92II CATG 3 cut(s) 421, 482, 715
HspAI GCGC 1 cut(s) 631
Kzo9I GATC 3 cut(s) 300, 349, 756
Lsp1109I GCAGC 5 cut(s) 178, 499, 517, 521, 640
LweI GCATC 3 cut(s) 256, 542, 773
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 2 cut(s) 353, 516
MaeII ACGT 2 cut(s) 45, 207
MaeIII GTNAC 5 cut(s) 19, 67, 214, 420, 727
MalI GATC 3 cut(s) 302, 351, 758
MboI GATC 3 cut(s) 300, 349, 756
MboII GAAGA 6 cut(s) 52, 385, 410, 413, 496, 759
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 2 cut(s) 300, 349
MluCI AATT 3 cut(s) 170, 778, 856
MlyI GAGTC 2 cut(s) 350, 602
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 4 cut(s) 85, 516, 574, 580
MseI TTAA 3 cut(s) 36, 504, 570
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 4 cut(s) 292, 635, 690, 804
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 4 cut(s) 292, 635, 690, 804
MwoI GCNNNNNNNGC 2 cut(s) 257, 266
NdeII GATC 3 cut(s) 300, 349, 756
NlaIII CATG 3 cut(s) 421, 482, 715
NmuCI GTSAC 3 cut(s) 67, 420, 727
NspI RCATGY 1 cut(s) 482
PfeI GAWTC 2 cut(s) 57, 107
PkrI GCNGC 5 cut(s) 168, 511, 514, 532, 630
PleI GAGTC 2 cut(s) 350, 601
PpsI GAGTC 2 cut(s) 350, 601
PshBI ATTAAT 1 cut(s) 504
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 4 cut(s) 290, 633, 688, 802
PspEI GGTNACC 1 cut(s) 727
PspGI CCWGG 4 cut(s) 290, 633, 688, 802
PspPI GGNCC 1 cut(s) 800
PstNI CAGNNNCTG 1 cut(s) 143
PsuI RGATCY 2 cut(s) 300, 349
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 3 cut(s) 444, 687, 737
RsaNI GTAC 3 cut(s) 443, 686, 736
RseI CAYNNNNRTG 1 cut(s) 716
SaqAI TTAA 3 cut(s) 36, 504, 570
SatI GCNGC 5 cut(s) 167, 510, 513, 531, 629
Sau3AI GATC 3 cut(s) 300, 349, 756
Sau96I GGNCC 1 cut(s) 800
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 2 cut(s) 350, 602
ScrFI CCNGG 4 cut(s) 292, 635, 690, 804
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 3 cut(s) 256, 542, 773
SfcI CTRYAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 716
Sse9I AATT 3 cut(s) 170, 778, 856
SsiI CCGC 1 cut(s) 40
SspMI CTAG 2 cut(s) 353, 516
StyD4I CCNGG 4 cut(s) 290, 633, 688, 802
StyI CCWWGG 2 cut(s) 128, 198
TaaI ACNGT 3 cut(s) 25, 124, 220
TaiI ACGT 2 cut(s) 48, 210
TaqI TCGA 2 cut(s) 465, 741
TasI AATT 3 cut(s) 170, 778, 856
TatI WGTACW 2 cut(s) 442, 735
TfiI GAWTC 2 cut(s) 57, 107
Tru1I TTAA 3 cut(s) 36, 504, 570
Tru9I TTAA 3 cut(s) 36, 504, 570
TscAI CASTG 1 cut(s) 129
TseFI GTSAC 3 cut(s) 67, 420, 727
TseI GCWGC 5 cut(s) 166, 509, 512, 530, 628
Tsp45I GTSAC 3 cut(s) 67, 420, 727
TspDTI ATGAA 3 cut(s) 509, 764, 778
TspGWI ACGGA 2 cut(s) 55, 813
TspRI CASTG 1 cut(s) 129
VpaK11BI GGWCC 1 cut(s) 800
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 1 cut(s) 856
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 1 cut(s) 482
XspI CTAG 2 cut(s) 353, 516
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.