Rh4BG391300

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003994
Physical Location & Seq
Reverse (-)
54402464 .. 54406399
3936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG391300.1

Sequence Viewer

Length: 771 bp
ATGAATTTTCACTCTGATTTGAAATTTACGCTCGCAATAGCTGGTCATCTCTCTCTACGAGTGCAGCAATTGGATGTTCGCTGTGAAACGAAGACCAAGGATAACGTTTTTGTTACTGTGGTTGCTTCTATTCAATACCGAGCTTTAGCTGACAAAGCTTCAGATGCCTTCTACAAGCTCAGCAATACCAGGGGGCAGATCCAGTCTTATGTCTTTGATGTTATTAGGGCAAGTGTTCCAAAGTTGGATCTAGACTCAACCTTTGAACAGAAGAATGATATCGCCAAAGCTGTGGAAGAAGAACTTGAAAAGGCCATGTCACATTATGGGTTTGAGATAGTACAAACATTGATTGTGGATATCGAACCAGATGAGCATGTGAAGAGAGCGATGAATGAGATTAATGCAGCTGCTAGGATGAGGTTGGCTGCATCTGAGAGAGCTGAAGGAGATAAGATACTACAGATTAAGCGAGCTGAGGGAGAGGCAGAGTCCAAATATCTGTCAGGGCTTGGCATAGCAAGGCAGCGCCAGGCTATTGTGGACGGGCTGAGAGACAGCGTGCTGGCTTTCTCTGAGAATGTACCTGGGACATCATCAAAGGATGTCATGGATATGGTTCTGGTGACCCAGTACTTCGATACAATGAAAGAGATCGGTGCATCTTCAAAGTCCAATTCGGTTTTCATCCCACACGGACCTGGTGCTGTGAAAGACATTGCTTCACAGATAAGAGACGGTCTCCTTCAAGGAAATTCAACTCACCAGTAA

Protein Analysis

256

Amino Acids

28.31

Weight (kDa)

5.74

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 17 - 152 7.6e-20 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 105
AclWI GGATC 2 cut(s) 193, 255
AcsI RAATTY 3 cut(s) 4, 23, 754
AcuI CTGAAG 2 cut(s) 144, 465
AfaI GTAC 3 cut(s) 342, 585, 635
AgsI TTSAA 7 cut(s) 22, 134, 266, 308, 669, 749, 759
AjnI CCWGG 4 cut(s) 188, 531, 586, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 9 cut(s) 41, 143, 149, 158, 178, 290, 410, 443, 476
AluI AGCT 9 cut(s) 41, 143, 149, 158, 178, 290, 410, 443, 476
Alw26I GTCTC 3 cut(s) 549, 729, 746
AlwI GGATC 2 cut(s) 193, 255
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 5 cut(s) 64, 407, 410, 428, 526
ApoI RAATTY 3 cut(s) 4, 23, 754
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AseI ATTAAT 1 cut(s) 402
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 637, 755
AvaII GGWCC 1 cut(s) 698
BbsI GAAGAC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 76, 397, 415, 419, 538
BciT130I CCWGG 4 cut(s) 190, 533, 588, 702
BcoDI GTCTC 3 cut(s) 549, 729, 746
BfaI CTAG 2 cut(s) 251, 414
BfmI CTRYAG 1 cut(s) 461
BfoI RGCGCY 1 cut(s) 532
BisI GCNGC 5 cut(s) 65, 408, 411, 429, 527
BlpI GCTNAGC 1 cut(s) 179
BlsI GCNGC 5 cut(s) 66, 409, 412, 430, 528
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 4 cut(s) 190, 533, 588, 702
Bme18I GGWCC 1 cut(s) 698
BmgT120I GGNCC 1 cut(s) 698
BmrFI CCNGG 4 cut(s) 190, 533, 588, 702
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 3 cut(s) 154, 440, 671
BmuI ACTGGG 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 726, 758
Bpu10I CCTNAGC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 3 cut(s) 96, 189, 587
Bse1I ACTGG 3 cut(s) 202, 631, 766
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 4 cut(s) 190, 533, 588, 702
BseDI CCNNGG 3 cut(s) 96, 189, 587
BseGI GGATG 4 cut(s) 79, 423, 610, 687
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 5 cut(s) 193, 426, 468, 542, 567
BseNI ACTGG 3 cut(s) 202, 631, 766
BseXI GCAGC 5 cut(s) 76, 397, 415, 419, 538
BsgI GTGCAG 1 cut(s) 83
BshFI GGCC 1 cut(s) 314
BslFI GGGAC 1 cut(s) 604
BsmAI GTCTC 3 cut(s) 549, 729, 746
BsmBI CGTCTC 1 cut(s) 729
BsmFI GGGAC 1 cut(s) 604
BsnI GGCC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 746
Bsp143I GATC 3 cut(s) 198, 247, 654
Bsp1720I GCTNAGC 1 cut(s) 179
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 5 cut(s) 192, 427, 469, 543, 568
BspPI GGATC 2 cut(s) 193, 255
BspTNI GGTCTC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 3 cut(s) 202, 631, 766
BssECI CCNNGG 3 cut(s) 96, 189, 587
BssMI GATC 3 cut(s) 198, 247, 654
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 4 cut(s) 190, 533, 588, 702
Bst4CI ACNGT 2 cut(s) 118, 740
Bst6I CTCTTC 1 cut(s) 377
BstC8I GCNNGC 4 cut(s) 33, 474, 563, 567
BstDEI CTNAG 5 cut(s) 179, 435, 477, 551, 576
BstEII GGTNACC 1 cut(s) 625
BstF5I GGATG 4 cut(s) 79, 423, 610, 687
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 201, 250, 657
BstMAI GTCTC 3 cut(s) 549, 729, 746
BstMBI GATC 3 cut(s) 198, 247, 654
BstMWI GCNNNNNNNGC 2 cut(s) 155, 164
BstNI CCWGG 4 cut(s) 190, 533, 588, 702
BstNSI RCATGY 1 cut(s) 380
BstPI GGTNACC 1 cut(s) 625
BstSCI CCNGG 4 cut(s) 188, 531, 586, 700
BstSFI CTRYAG 1 cut(s) 461
BstV1I GCAGC 5 cut(s) 76, 397, 415, 419, 538
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 198, 247
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 2 cut(s) 198, 247
BsuRI GGCC 1 cut(s) 314
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 4 cut(s) 79, 423, 610, 687
Cac8I GCNNGC 4 cut(s) 33, 474, 563, 567
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 698
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 3 cut(s) 341, 584, 634
CviAII CATG 3 cut(s) 316, 377, 610
CviQI GTAC 3 cut(s) 341, 584, 634
DdeI CTNAG 5 cut(s) 179, 435, 477, 551, 576
DpnI GATC 3 cut(s) 200, 249, 656
DpnII GATC 3 cut(s) 198, 247, 654
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 746
Eco32I GATATC 2 cut(s) 280, 361
Eco47I GGWCC 1 cut(s) 698
Eco57I CTGAAG 2 cut(s) 144, 465
Eco91I GGTNACC 1 cut(s) 625
EcoO65I GGTNACC 1 cut(s) 625
EcoRII CCWGG 4 cut(s) 188, 531, 586, 700
EcoRV GATATC 2 cut(s) 280, 361
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 729
FaeI CATG 3 cut(s) 319, 380, 613
FaiI YATR 7 cut(s) 210, 317, 327, 378, 518, 611, 617
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 1 cut(s) 604
FatI CATG 3 cut(s) 315, 376, 609
Fnu4HI GCNGC 5 cut(s) 65, 408, 411, 429, 527
FokI GGATG 4 cut(s) 86, 430, 617, 674
Fsp4HI GCNGC 5 cut(s) 65, 408, 411, 429, 527
FspBI CTAG 2 cut(s) 251, 414
GlaI GCGC 1 cut(s) 530
GluI GCNGC 5 cut(s) 65, 408, 411, 429, 527
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 1 cut(s) 314
HhaI GCGC 1 cut(s) 531
Hin1II CATG 3 cut(s) 319, 380, 613
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 2 cut(s) 254, 491
HphI GGTGA 2 cut(s) 637, 755
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 16, 163, 436, 577
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 3 cut(s) 178, 440, 755
HpyCH4III ACNGT 2 cut(s) 118, 740
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 4 cut(s) 64, 407, 431, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 164
HpyF3I CTNAG 5 cut(s) 179, 435, 477, 551, 576
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 3 cut(s) 319, 380, 613
HspAI GCGC 1 cut(s) 529
Kzo9I GATC 3 cut(s) 198, 247, 654
Lsp1109I GCAGC 5 cut(s) 76, 397, 415, 419, 538
LweI GCATC 3 cut(s) 154, 440, 671
MabI ACCWGGT 1 cut(s) 700
MaeI CTAG 2 cut(s) 251, 414
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 112, 318, 625
MalI GATC 3 cut(s) 200, 249, 656
MboI GATC 3 cut(s) 198, 247, 654
MboII GAAGA 6 cut(s) 103, 283, 308, 311, 394, 657
MfeI CAATTG 1 cut(s) 68
MflI RGATCY 2 cut(s) 198, 247
MluCI AATT 5 cut(s) 4, 23, 68, 676, 754
MlyI GAGTC 2 cut(s) 248, 500
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 3 cut(s) 414, 472, 478
MseI TTAA 2 cut(s) 402, 468
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 410
MspR9I CCNGG 4 cut(s) 190, 533, 588, 702
MunI CAATTG 1 cut(s) 68
MvaI CCWGG 4 cut(s) 190, 533, 588, 702
MwoI GCNNNNNNNGC 2 cut(s) 155, 164
NdeII GATC 3 cut(s) 198, 247, 654
NlaIII CATG 3 cut(s) 319, 380, 613
NmuCI GTSAC 2 cut(s) 318, 625
NspI RCATGY 1 cut(s) 380
PkrI GCNGC 5 cut(s) 66, 409, 412, 430, 528
PleI GAGTC 2 cut(s) 248, 499
PpsI GAGTC 2 cut(s) 248, 499
PshBI ATTAAT 1 cut(s) 402
Psp1406I AACGTT 1 cut(s) 105
Psp6I CCWGG 4 cut(s) 188, 531, 586, 700
PspEI GGTNACC 1 cut(s) 625
PspGI CCWGG 4 cut(s) 188, 531, 586, 700
PspPI GGNCC 1 cut(s) 698
PsuI RGATCY 2 cut(s) 198, 247
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 3 cut(s) 342, 585, 635
RsaNI GTAC 3 cut(s) 341, 584, 634
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 2 cut(s) 402, 468
SatI GCNGC 5 cut(s) 65, 408, 411, 429, 527
Sau3AI GATC 3 cut(s) 198, 247, 654
Sau96I GGNCC 1 cut(s) 698
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 2 cut(s) 248, 500
ScrFI CCNGG 4 cut(s) 190, 533, 588, 702
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 3 cut(s) 154, 440, 671
SfcI CTRYAG 1 cut(s) 461
SinI GGWCC 1 cut(s) 698
SmiMI CAYNNNNRTG 1 cut(s) 614
Sse9I AATT 5 cut(s) 4, 23, 68, 676, 754
SspMI CTAG 2 cut(s) 251, 414
StyD4I CCNGG 4 cut(s) 188, 531, 586, 700
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 118, 740
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 363, 639
TasI AATT 5 cut(s) 4, 23, 68, 676, 754
TatI WGTACW 2 cut(s) 340, 633
Tru1I TTAA 2 cut(s) 402, 468
Tru9I TTAA 2 cut(s) 402, 468
TseFI GTSAC 2 cut(s) 318, 625
TseI GCWGC 5 cut(s) 64, 407, 410, 428, 526
Tsp45I GTSAC 2 cut(s) 318, 625
TspDTI ATGAA 4 cut(s) 17, 407, 662, 676
TspGWI ACGGA 1 cut(s) 711
VpaK11BI GGWCC 1 cut(s) 698
VspI ATTAAT 1 cut(s) 402
XapI RAATTY 3 cut(s) 4, 23, 754
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 1 cut(s) 380
XspI CTAG 2 cut(s) 251, 414
ZrmI AGTACT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.