Rorug04G0326800

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003994
Physical Location & Seq
Forward (+)
50175167 .. 50175765
599 bp
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UTR
Exon/CDS
Intron
Rorug04G0326800.1

Sequence Viewer

Length: 480 bp
ATGGTCACAGAAGGCAACCACCAAATCGAACGCCTTCCCGTTGACTCCCCGGCCGATTTCATAGCCTTCAATGCTAGGTGGGCAATGCCGTACAAAGAGAGCGGCTCCACCTCAAACTTGTATTACTCTTTCGAAGTTGCTGGGACTCACATCATCATGCTTGGGTCCTATGCCGAGTACCACTCCGAGTCGGAGCAGTACTCGTGGCTTGTAGCCGATTTGGCAAAGATTGATAGAAAGAGAACGCCGTGGGTTGTTGTGGTATTGCATGCACCGTGGTATAATACTAACACTGCTCATCAGGGTGATGAAGAGGGCATGAGGGTTGCTATGGAAGAGTTGCTGTATAAGGCACGGGTTGATTTGGTTTATTCTGGACATGTTCATGCCTATGAACGGTTTGTAACCATGTTCATGCCTATGAACGGTTTACTAGGGTATACAACAATGAGTCAGACCCTTGTGGTCCGGTGCATGTAA

Protein Analysis

159

Amino Acids

18.27

Weight (kDa)

5.58

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 2 - 131 6.3e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 440
AciI CCGC 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 51
AfaI GTAC 3 cut(s) 92, 179, 200
AfiI CCNNNNNNNGG 2 cut(s) 396, 425
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 1 cut(s) 70
AoxI GGCC 1 cut(s) 51
Asp700I GAANNNNTTC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 165, 466
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 317
AsuII TTCGAA 1 cut(s) 132
AvaII GGWCC 2 cut(s) 165, 466
BauI CACGAG 1 cut(s) 202
BceAI ACGGC 2 cut(s) 73, 232
BcnI CCSGG 1 cut(s) 50
BfaI CTAG 2 cut(s) 75, 434
BglI GCCNNNNNGGC 1 cut(s) 221
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 2 cut(s) 165, 466
BmgT120I GGNCC 2 cut(s) 165, 466
BmiI GGNNCC 2 cut(s) 106, 166
BmrFI CCNGG 1 cut(s) 50
BplI GAGNNNNNCTC 6 cut(s) 89, 121, 167, 199, 185, 217
Bpu14I TTCGAA 1 cut(s) 132
BpuMI CCSGG 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 48, 248, 275
BsaWI WCCGGW 1 cut(s) 468
Bsc4I CCNNNNNNNGG 2 cut(s) 396, 425
Bse3DI GCAATG 1 cut(s) 90
BseDI CCNNGG 3 cut(s) 48, 248, 275
BseLI CCNNNNNNNGG 2 cut(s) 396, 425
BseMI GCAATG 1 cut(s) 90
BseX3I CGGCCG 1 cut(s) 51
BseYI CCCAGC 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 1 cut(s) 53
BsiEI CGRYCG 1 cut(s) 54
BsiSI CCGG 2 cut(s) 50, 469
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 2 cut(s) 396, 425
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 1 cut(s) 53
Bsp119I TTCGAA 1 cut(s) 132
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 106, 166
BspT104I TTCGAA 1 cut(s) 132
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 90
BssECI CCNNGG 3 cut(s) 48, 248, 275
BssNAI GTATAC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 202
Bst1107I GTATAC 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 276, 399, 428
Bst6I CTCTTC 2 cut(s) 306, 330
BstBI TTCGAA 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 270
BstDSI CCRYGG 2 cut(s) 248, 275
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 71, 80, 221
BstNSI RCATGY 3 cut(s) 272, 383, 478
BstSCI CCNGG 1 cut(s) 48
BstZ17I GTATAC 1 cut(s) 441
BstZI CGGCCG 1 cut(s) 51
BsuRI GGCC 1 cut(s) 53
BtgI CCRYGG 2 cut(s) 248, 275
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 2 cut(s) 165, 466
Csp6I GTAC 3 cut(s) 91, 178, 199
CviAII CATG 8 cut(s) 157, 269, 319, 380, 386, 409, 415, 475
CviJI RGCY 5 cut(s) 53, 65, 105, 208, 215
CviKI_1 RGCY 5 cut(s) 53, 65, 105, 208, 215
CviQI GTAC 3 cut(s) 91, 178, 199
EaeI YGGCCR 1 cut(s) 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 306, 330
EarI CTCTTC 2 cut(s) 306, 330
EclXI CGGCCG 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 165, 466
Eco52I CGGCCG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 165
FaeI CATG 8 cut(s) 160, 272, 322, 383, 389, 412, 418, 478
FaqI GGGAC 1 cut(s) 157
FatI CATG 8 cut(s) 156, 268, 318, 379, 385, 408, 414, 474
FblI GTMKAC 1 cut(s) 440
Fnu4HI GCNGC 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 2 cut(s) 75, 434
GluI GCNGC 1 cut(s) 103
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 1 cut(s) 53
HapII CCGG 2 cut(s) 50, 469
Hin1II CATG 8 cut(s) 160, 272, 322, 383, 389, 412, 418, 478
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 4 cut(s) 44, 145, 188, 451
HpaII CCGG 2 cut(s) 50, 469
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 43, 431, 441
Hpy188I TCNGA 3 cut(s) 187, 193, 456
Hpy188III TCNNGA 1 cut(s) 375
Hpy8I GTNNAC 3 cut(s) 43, 431, 441
HpyAV CCTTC 3 cut(s) 5, 44, 76
HpyCH4III ACNGT 3 cut(s) 276, 399, 428
HpyCH4V TGCA 3 cut(s) 268, 272, 474
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 80, 221
Hsp92II CATG 8 cut(s) 160, 272, 322, 383, 389, 412, 418, 478
LmnI GCTCC 2 cut(s) 110, 193
LpnPI CCDG 4 cut(s) 63, 126, 287, 360
MaeI CTAG 2 cut(s) 75, 434
MaeIII GTNAC 2 cut(s) 4, 403
MbiI CCGCTC 1 cut(s) 102
MboII GAAGA 2 cut(s) 323, 347
MlyI GAGTC 4 cut(s) 38, 139, 197, 460
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 3 cut(s) 121, 307, 315
MroXI GAANNNNTTC 1 cut(s) 33
MslI CAYNNNNRTG 6 cut(s) 155, 303, 384, 390, 413, 419
MspI CCGG 2 cut(s) 50, 469
MspR9I CCNGG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 71, 80, 221
NciI CCSGG 1 cut(s) 50
NlaIII CATG 8 cut(s) 160, 272, 322, 383, 389, 412, 418, 478
NlaIV GGNNCC 2 cut(s) 106, 166
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 3 cut(s) 272, 383, 478
NspV TTCGAA 1 cut(s) 132
PaeI GCATGC 1 cut(s) 272
PciI ACATGT 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 4 cut(s) 38, 139, 196, 459
PpsI GAGTC 4 cut(s) 38, 139, 196, 459
PpuMI RGGWCCY 1 cut(s) 165
PscI ACATGT 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 106, 166
PspPI GGNCC 2 cut(s) 165, 466
PspPPI RGGWCCY 1 cut(s) 165
RsaI GTAC 3 cut(s) 92, 179, 200
RsaNI GTAC 3 cut(s) 91, 178, 199
RseI CAYNNNNRTG 6 cut(s) 155, 303, 384, 390, 413, 419
SatI GCNGC 1 cut(s) 103
Sau96I GGNCC 2 cut(s) 165, 466
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 4 cut(s) 38, 139, 197, 460
ScrFI CCNGG 1 cut(s) 50
SetI ASST 2 cut(s) 80, 113
SfuI TTCGAA 1 cut(s) 132
SinI GGWCC 2 cut(s) 165, 466
SmiMI CAYNNNNRTG 6 cut(s) 155, 303, 384, 390, 413, 419
SphI GCATGC 1 cut(s) 272
SsiI CCGC 1 cut(s) 102
SspMI CTAG 2 cut(s) 75, 434
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 276, 399, 428
TaqI TCGA 2 cut(s) 27, 132
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 105
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 6 cut(s) 49, 324, 374, 403, 408, 437
TspRI CASTG 1 cut(s) 298
VpaK11BI GGWCC 2 cut(s) 165, 466
XceI RCATGY 3 cut(s) 272, 383, 478
XmiI GTMKAC 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 2 cut(s) 75, 434
ZrmI AGTACT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.