Rh4DG385200

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003994
Physical Location & Seq
Reverse (-)
60232550 .. 60234509
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG385200.1

Sequence Viewer

Length: 861 bp
ATGGGACAGTGCCTTGGTTGTATCCAGGTGGACCAGTCTACTGTTGCAATAAGAGAAACTTTTGGGAAGTTTGATGATGTGCTCGAACCTGGTTGTCATTGTTTGCCTTGGTGTTTGGGTAGCGCAGTAGCTGGTCATCTCTCTCTACGAGTGCAGCAATTGGATGTTCGCTGTGAAACAAAGACCAAGGATAACGTTTTTGTTACTGTGGTTGCTTCTATTCAATACCGAGCTTTAGCTGACAAAGCTTCAGATGCCTTCTACAAGCTCAGCAATACCAGGGGGCAGATCCAGTCTTATGTCTTTGATGTTATTAGGGCAAGTGTTCCAAAGTTGGATCTAGACTCAACCTTTGAACAGAAGAATGATATCGCCAAAGCTGTGGAAGAAGAACTTGAAAAGGCCATGTCACATTATGGGTTTGAGATAGTACAAACATTGATTGTGGATATCGAACCAGATGAGCATGTGAAGAGAGCGATGAATGAGATTAATGCAGCTGCTAGGATGAGGTTGGCTGCATCTGAGAGAGCTGAAGGAGATAAGATACTACAGATTAAGCGAGCTGAGGGAGAGGCAGAGTCCAAATATCTGTCAGGGCTTGGCATAGCAAGGCAGCGCCAGGCTATTGTGGACGGGCTGAGAGACAGCGTGCTGGCTTTCTCTGAGAATGTACCTGGGACATCATCAAAGGATGTCATGGATATGGTTCTGGTGACCCAGTACTTCGATACAATGAAAGAGATCGGTGCATCTTCAAAGTCCAATTCAGTTTTCATCCCACATGGACCTGGTGCTGTGAAAGACATTGCTTCACAGATAAGAGATGGTCTCCTTCAAGGAAATTCAACTCACCAGTAA

Protein Analysis

286

Amino Acids

31.4

Weight (kDa)

5.35

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 9 - 182 4.6e-28 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 38
AclI AACGTT 1 cut(s) 195
AclWI GGATC 2 cut(s) 283, 345
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 2 cut(s) 234, 555
AfaI GTAC 3 cut(s) 432, 675, 725
AgsI TTSAA 6 cut(s) 224, 356, 398, 759, 839, 849
AjnI CCWGG 6 cut(s) 24, 88, 278, 621, 676, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 758, 790
AluBI AGCT 9 cut(s) 131, 233, 239, 248, 268, 380, 500, 533, 566
AluI AGCT 9 cut(s) 131, 233, 239, 248, 268, 380, 500, 533, 566
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 639, 836
AlwI GGATC 2 cut(s) 283, 345
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 5 cut(s) 154, 497, 500, 518, 616
ApoI RAATTY 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 2 cut(s) 125, 621
AspS9I GGNCC 2 cut(s) 31, 788
AsuHPI GGTGA 2 cut(s) 727, 845
AvaII GGWCC 2 cut(s) 31, 788
Bbv12I GWGCWC 1 cut(s) 84
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 166, 487, 505, 509, 628
BccI CCATC 1 cut(s) 821
BciT130I CCWGG 6 cut(s) 26, 90, 280, 623, 678, 792
BciVI GTATCC 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 639, 836
BfaI CTAG 2 cut(s) 341, 504
BfmI CTRYAG 1 cut(s) 551
BfoI RGCGCY 1 cut(s) 622
BfuI GTATCC 1 cut(s) 32
BisI GCNGC 5 cut(s) 155, 498, 501, 519, 617
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 5 cut(s) 156, 499, 502, 520, 618
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 6 cut(s) 26, 90, 280, 623, 678, 792
Bme18I GGWCC 2 cut(s) 31, 788
BmgT120I GGNCC 2 cut(s) 31, 788
BmrFI CCNGG 6 cut(s) 26, 90, 280, 623, 678, 792
BmrI ACTGGG 1 cut(s) 715
BmsI GCATC 3 cut(s) 244, 530, 761
BmuI ACTGGG 1 cut(s) 715
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 5 cut(s) 13, 107, 186, 279, 677
Bse1I ACTGG 4 cut(s) 34, 292, 721, 856
Bse3DI GCAATG 1 cut(s) 807
BseBI CCWGG 6 cut(s) 26, 90, 280, 623, 678, 792
BseDI CCNNGG 5 cut(s) 13, 107, 186, 279, 677
BseGI GGATG 4 cut(s) 169, 513, 700, 777
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 5 cut(s) 283, 516, 558, 632, 657
BseNI ACTGG 4 cut(s) 34, 292, 721, 856
BseXI GCAGC 5 cut(s) 166, 487, 505, 509, 628
BsgI GTGCAG 1 cut(s) 173
BshFI GGCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 84
BslFI GGGAC 2 cut(s) 18, 694
BsmAI GTCTC 2 cut(s) 639, 836
BsmFI GGGAC 2 cut(s) 18, 694
BsnI GGCC 1 cut(s) 404
Bso31I GGTCTC 1 cut(s) 836
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 288, 337, 744
Bsp1720I GCTNAGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 5 cut(s) 282, 517, 559, 633, 658
BspPI GGATC 2 cut(s) 283, 345
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 807
BsrI ACTGG 4 cut(s) 34, 292, 721, 856
BssECI CCNNGG 5 cut(s) 13, 107, 186, 279, 677
BssMI GATC 3 cut(s) 288, 337, 744
BssT1I CCWWGG 3 cut(s) 13, 107, 186
Bst2UI CCWGG 6 cut(s) 26, 90, 280, 623, 678, 792
Bst4CI ACNGT 3 cut(s) 9, 43, 208
Bst6I CTCTTC 1 cut(s) 467
BstC8I GCNNGC 3 cut(s) 564, 653, 657
BstDEI CTNAG 5 cut(s) 269, 525, 567, 641, 666
BstEII GGTNACC 1 cut(s) 715
BstF5I GGATG 4 cut(s) 169, 513, 700, 777
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 2 cut(s) 125, 621
BstKTI GATC 3 cut(s) 291, 340, 747
BstMAI GTCTC 2 cut(s) 639, 836
BstMBI GATC 3 cut(s) 288, 337, 744
BstMWI GCNNNNNNNGC 2 cut(s) 245, 254
BstNI CCWGG 6 cut(s) 26, 90, 280, 623, 678, 792
BstNSI RCATGY 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 715
BstSCI CCNGG 6 cut(s) 24, 88, 278, 621, 676, 790
BstSFI CTRYAG 1 cut(s) 551
BstV1I GCAGC 5 cut(s) 166, 487, 505, 509, 628
BstX2I RGATCY 2 cut(s) 288, 337
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 2 cut(s) 288, 337
BsuI GTATCC 1 cut(s) 32
BsuRI GGCC 1 cut(s) 404
BtgZI GCGATG 1 cut(s) 494
BtsCI GGATG 4 cut(s) 169, 513, 700, 777
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 564, 653, 657
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 2 cut(s) 125, 621
Cfr13I GGNCC 2 cut(s) 31, 788
CsiI ACCWGGT 2 cut(s) 88, 790
Csp6I GTAC 3 cut(s) 431, 674, 724
CviAII CATG 4 cut(s) 406, 467, 700, 785
CviQI GTAC 3 cut(s) 431, 674, 724
DdeI CTNAG 5 cut(s) 269, 525, 567, 641, 666
DpnI GATC 3 cut(s) 290, 339, 746
DpnII GATC 3 cut(s) 288, 337, 744
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco130I CCWWGG 3 cut(s) 13, 107, 186
Eco31I GGTCTC 1 cut(s) 836
Eco32I GATATC 2 cut(s) 370, 451
Eco47I GGWCC 2 cut(s) 31, 788
Eco57I CTGAAG 2 cut(s) 234, 555
Eco91I GGTNACC 1 cut(s) 715
EcoO65I GGTNACC 1 cut(s) 715
EcoRII CCWGG 6 cut(s) 24, 88, 278, 621, 676, 790
EcoRV GATATC 2 cut(s) 370, 451
EcoT14I CCWWGG 3 cut(s) 13, 107, 186
ErhI CCWWGG 3 cut(s) 13, 107, 186
FaeI CATG 4 cut(s) 409, 470, 703, 788
FaiI YATR 8 cut(s) 300, 407, 417, 468, 608, 701, 707, 786
FalI AAGNNNNNCTT 4 cut(s) 43, 75, 378, 410
FaqI GGGAC 2 cut(s) 18, 694
FatI CATG 4 cut(s) 405, 466, 699, 784
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 5 cut(s) 155, 498, 501, 519, 617
FokI GGATG 4 cut(s) 176, 520, 707, 764
Fsp4HI GCNGC 5 cut(s) 155, 498, 501, 519, 617
FspBI CTAG 2 cut(s) 341, 504
GlaI GCGC 2 cut(s) 124, 620
GluI GCNGC 5 cut(s) 155, 498, 501, 519, 617
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 1 cut(s) 404
HhaI GCGC 2 cut(s) 125, 621
Hin1II CATG 4 cut(s) 409, 470, 703, 788
Hin6I GCGC 2 cut(s) 123, 619
HinP1I GCGC 2 cut(s) 123, 619
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 2 cut(s) 344, 581
HphI GGTGA 2 cut(s) 727, 845
Hpy166II GTNNAC 3 cut(s) 31, 39, 634
Hpy188I TCNGA 3 cut(s) 253, 526, 667
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 3 cut(s) 31, 39, 634
HpyAV CCTTC 3 cut(s) 268, 530, 845
HpyCH4III ACNGT 3 cut(s) 9, 43, 208
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 5 cut(s) 47, 154, 497, 521, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 254
HpyF3I CTNAG 5 cut(s) 269, 525, 567, 641, 666
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 4 cut(s) 409, 470, 703, 788
HspAI GCGC 2 cut(s) 123, 619
Kzo9I GATC 3 cut(s) 288, 337, 744
Lsp1109I GCAGC 5 cut(s) 166, 487, 505, 509, 628
LweI GCATC 3 cut(s) 244, 530, 761
MabI ACCWGGT 2 cut(s) 88, 790
MaeI CTAG 2 cut(s) 341, 504
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 202, 408, 715
MalI GATC 3 cut(s) 290, 339, 746
MboI GATC 3 cut(s) 288, 337, 744
MboII GAAGA 5 cut(s) 373, 398, 401, 484, 747
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 2 cut(s) 288, 337
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 3 cut(s) 158, 766, 844
MlyI GAGTC 2 cut(s) 338, 590
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 3 cut(s) 504, 562, 568
MseI TTAA 2 cut(s) 492, 558
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 6 cut(s) 26, 90, 280, 623, 678, 792
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 6 cut(s) 26, 90, 280, 623, 678, 792
MwoI GCNNNNNNNGC 2 cut(s) 245, 254
NdeII GATC 3 cut(s) 288, 337, 744
NlaIII CATG 4 cut(s) 409, 470, 703, 788
NmuCI GTSAC 2 cut(s) 408, 715
NspI RCATGY 1 cut(s) 470
PkrI GCNGC 5 cut(s) 156, 499, 502, 520, 618
PleI GAGTC 2 cut(s) 338, 589
PpsI GAGTC 2 cut(s) 338, 589
PshBI ATTAAT 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 195
Psp6I CCWGG 6 cut(s) 24, 88, 278, 621, 676, 790
PspEI GGTNACC 1 cut(s) 715
PspGI CCWGG 6 cut(s) 24, 88, 278, 621, 676, 790
PspPI GGNCC 2 cut(s) 31, 788
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 2 cut(s) 288, 337
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 3 cut(s) 432, 675, 725
RsaNI GTAC 3 cut(s) 431, 674, 724
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 2 cut(s) 492, 558
SatI GCNGC 5 cut(s) 155, 498, 501, 519, 617
Sau3AI GATC 3 cut(s) 288, 337, 744
Sau96I GGNCC 2 cut(s) 31, 788
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 2 cut(s) 338, 590
ScrFI CCNGG 6 cut(s) 26, 90, 280, 623, 678, 792
SduI GDGCHC 1 cut(s) 84
SexAI ACCWGGT 2 cut(s) 88, 790
SfaNI GCATC 3 cut(s) 244, 530, 761
SfcI CTRYAG 1 cut(s) 551
SinI GGWCC 2 cut(s) 31, 788
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 3 cut(s) 158, 766, 844
SspMI CTAG 2 cut(s) 341, 504
StyD4I CCNGG 6 cut(s) 24, 88, 278, 621, 676, 790
StyI CCWWGG 3 cut(s) 13, 107, 186
TaaI ACNGT 3 cut(s) 9, 43, 208
TaiI ACGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 84, 453, 729
TasI AATT 3 cut(s) 158, 766, 844
TatI WGTACW 2 cut(s) 430, 723
Tru1I TTAA 2 cut(s) 492, 558
Tru9I TTAA 2 cut(s) 492, 558
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 2 cut(s) 408, 715
TseI GCWGC 5 cut(s) 154, 497, 500, 518, 616
Tsp45I GTSAC 2 cut(s) 408, 715
TspDTI ATGAA 3 cut(s) 497, 752, 766
TspRI CASTG 1 cut(s) 14
VpaK11BI GGWCC 2 cut(s) 31, 788
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 2 cut(s) 341, 504
ZrmI AGTACT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.