MD13G1092700.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012761
Physical Location & Seq
Reverse (-)
6542902 .. 6543792
891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1092700.v1.1.491

Sequence Viewer

Length: 891 bp
ATGCAAGCGGTTTCCGTGTTTCCCGTGTCGCCGAGCTTCGAATCCCACGGTTCCATGAACGATTTCGAAGTCGAGTACGCGCAGCTCTCTGCGAATTTCAGCTCCAAGCTCCAAGTCGCCGGTTCCGGCGGCGATTTCGAATTCGTGGACGCTGAGAAGCCAGGAATGGAAGATAAAGAAGCTCAAATCGTGGACGGTGGCGGCGAATGCGAGAGCGAAAACGACGACGATTTCTCTTTCGCCCCTACGAACGCCGACGGGTCGCCGATTTCCGCGGAGGATATATTCCAGAACGGCCACTTCCGGCCGGTATATCCGATTTTCAACCGAGATCTCCTCTTCGCCGACGCTGACGACGGCGATGCTTCAAGAGCCGGAGCGGCTTCGTCCTCGTCTCTGCGGCCGCCATTGATGAAGCTCTTCTTCGAGGAACGGGACACGCCGTCGTCGTCGGCGTCCGAGTCGGACGAGCTCGAAGCAGTCCCGGAAGGAACGTACTGTGAGTGGTCCGGGAAGCCGGTGGAGCTCCGGAACAAGAGCAATTCCACGGGGTCCTCGAAGCTGTGGAGAGTCAGGGACTTGAAGCTCCGGAGCAACAGCGACGGAAAGGACGCATTTGTTTTCCTTAAACCTAAGTCCGCGGCGAGCCCGAAGCCGAGCCATGAATCGGCGGCGGATGAGAAGAGCAGCGTTAAGATTCAAAAGACGGTGGAGAAGTTAAAGGGGAAGGCTAAGAAAGTCGAAACGGTGTCGTCTGCGCACGAGAAGCATTACGTGAAGAACAGAGAGAAGAAGGAGGGGGACAAACGGCGGTCGTACTTGCCGTATCGACCGGTGGTTGGGTTCTTCACAAATGTGAACGGATTGAGCAGAAACGTCCATCCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

297

Amino Acids

32.5

Weight (kDa)

5.33

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 275 8.8e-29 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 759
AccBSI CCGCTC 1 cut(s) 380
AccII CGCG 3 cut(s) 80, 275, 641
AccIII TCCGGA 2 cut(s) 528, 588
AcoI YGGCCR 3 cut(s) 295, 305, 401
AcsI RAATTY 2 cut(s) 94, 140
AcyI GRCGYC 1 cut(s) 455
AfaI GTAC 3 cut(s) 77, 497, 818
AfiI CCNNNNNNNGG 2 cut(s) 667, 839
AgeI ACCGGT 1 cut(s) 832
AgsI TTSAA 4 cut(s) 325, 369, 583, 701
AhdI GACNNNNNGTC 1 cut(s) 442
AjnI CCWGG 1 cut(s) 160
AleI CACNNNNGTG 1 cut(s) 854
Alw21I GWGCWC 2 cut(s) 474, 528
Alw26I GTCTC 1 cut(s) 399
Aor13HI TCCGGA 2 cut(s) 528, 588
AoxI GGCC 3 cut(s) 295, 305, 401
ApeKI GCWGC 2 cut(s) 82, 687
ApoI RAATTY 2 cut(s) 94, 140
AsiGI ACCGGT 1 cut(s) 832
Asp700I GAANNNNTTC 2 cut(s) 62, 419
AspLEI GCGC 2 cut(s) 82, 760
AspS9I GGNCC 2 cut(s) 507, 552
AsuC2I CCSGG 2 cut(s) 485, 511
AsuII TTCGAA 3 cut(s) 39, 66, 138
AvaII GGWCC 2 cut(s) 507, 552
BanII GRGCYC 3 cut(s) 474, 528, 650
BauI CACGAG 1 cut(s) 761
Bbv12I GWGCWC 2 cut(s) 474, 528
BbvI GCAGC 2 cut(s) 94, 699
BccI CCATC 1 cut(s) 888
BceAI ACGGC 5 cut(s) 310, 373, 427, 808, 824
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 162
BcnI CCSGG 2 cut(s) 485, 511
BcoDI GTCTC 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 380
BglII AGATCT 1 cut(s) 331
BisI GCNGC 9 cut(s) 83, 130, 202, 381, 401, 404, 642, 672, 688
BlsI GCNGC 9 cut(s) 84, 131, 203, 382, 402, 405, 643, 673, 689
Bme1390I CCNGG 3 cut(s) 162, 485, 511
Bme18I GGWCC 2 cut(s) 507, 552
BmeRI GACNNNNNGTC 1 cut(s) 442
BmgT120I GGNCC 2 cut(s) 507, 552
BmiI GGNNCC 3 cut(s) 52, 124, 553
BmrFI CCNGG 3 cut(s) 162, 485, 511
BmsI GCATC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 3 cut(s) 39, 66, 138
BpuMI CCSGG 2 cut(s) 485, 511
BsaAI YACGTR 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 455
BsaJI CCNNGG 4 cut(s) 46, 273, 546, 639
BsaWI WCCGGW 3 cut(s) 528, 588, 832
Bsc4I CCNNNNNNNGG 2 cut(s) 667, 839
Bse118I RCCGGY 4 cut(s) 119, 307, 517, 832
BseAI TCCGGA 2 cut(s) 528, 588
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 4 cut(s) 46, 273, 546, 639
BseGI GGATG 2 cut(s) 682, 880
BseLI CCNNNNNNNGG 2 cut(s) 667, 839
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 326
BseX3I CGGCCG 2 cut(s) 305, 401
BseXI GCAGC 2 cut(s) 94, 699
Bsh1236I CGCG 3 cut(s) 80, 275, 641
Bsh1285I CGRYCG 4 cut(s) 308, 404, 815, 833
BshFI GGCC 3 cut(s) 297, 307, 403
BshTI ACCGGT 1 cut(s) 832
BsiEI CGRYCG 4 cut(s) 308, 404, 815, 833
BsiHKAI GWGCWC 2 cut(s) 474, 528
BslFI GGGAC 4 cut(s) 449, 467, 590, 815
BslI CCNNNNNNNGG 2 cut(s) 667, 839
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 4 cut(s) 449, 467, 590, 815
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 3 cut(s) 297, 307, 403
Bsp119I TTCGAA 3 cut(s) 39, 66, 138
Bsp1286I GDGCHC 3 cut(s) 474, 528, 650
Bsp13I TCCGGA 2 cut(s) 528, 588
Bsp143I GATC 1 cut(s) 331
BspANI GGCC 3 cut(s) 297, 307, 403
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 2 cut(s) 528, 588
BspFNI CGCG 3 cut(s) 80, 275, 641
BspLI GGNNCC 3 cut(s) 52, 124, 553
BspQI GCTCTTC 2 cut(s) 425, 677
BspT104I TTCGAA 3 cut(s) 39, 66, 138
BsrBI CCGCTC 1 cut(s) 380
BsrFI RCCGGY 4 cut(s) 119, 307, 517, 832
BssAI RCCGGY 4 cut(s) 119, 307, 517, 832
BssECI CCNNGG 4 cut(s) 46, 273, 546, 639
BssMI GATC 1 cut(s) 331
BssNI GRCGYC 1 cut(s) 455
BssSI CACGAG 1 cut(s) 761
Bst2BI CACGAG 1 cut(s) 761
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 5 cut(s) 50, 197, 500, 709, 748
Bst6I CTCTTC 3 cut(s) 344, 425, 677
BstACI GRCGYC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 775
BstBI TTCGAA 3 cut(s) 39, 66, 138
BstC8I GCNNGC 2 cut(s) 6, 646
BstDEI CTNAG 3 cut(s) 153, 633, 732
BstDSI CCRYGG 4 cut(s) 46, 273, 546, 639
BstF5I GGATG 2 cut(s) 682, 880
BstFNI CGCG 3 cut(s) 80, 275, 641
BstHHI GCGC 2 cut(s) 82, 760
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 1 cut(s) 331
BstMCI CGRYCG 4 cut(s) 308, 404, 815, 833
BstMWI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 523, 766
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 3 cut(s) 160, 483, 509
BstUI CGCG 3 cut(s) 80, 275, 641
BstV1I GCAGC 2 cut(s) 94, 699
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZI CGGCCG 2 cut(s) 305, 401
BsuRI GGCC 3 cut(s) 297, 307, 403
BtgI CCRYGG 4 cut(s) 46, 273, 546, 639
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 682, 880
Cac8I GCNNGC 2 cut(s) 6, 646
CciNI GCGGCCGC 1 cut(s) 401
CfoI GCGC 2 cut(s) 82, 760
Cfr10I RCCGGY 4 cut(s) 119, 307, 517, 832
Cfr13I GGNCC 2 cut(s) 507, 552
Cfr42I CCGCGG 2 cut(s) 276, 642
CseI GACGC 4 cut(s) 158, 356, 444, 620
Csp6I GTAC 3 cut(s) 76, 496, 817
CspAI ACCGGT 1 cut(s) 832
CviAII CATG 2 cut(s) 55, 662
CviQI GTAC 3 cut(s) 76, 496, 817
DdeI CTNAG 3 cut(s) 153, 633, 732
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 3 cut(s) 295, 305, 401
EagI CGGCCG 2 cut(s) 305, 401
Eam1104I CTCTTC 3 cut(s) 344, 425, 677
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 3 cut(s) 344, 425, 677
EciI GGCGGA 1 cut(s) 689
Ecl136II GAGCTC 2 cut(s) 472, 526
EclXI CGGCCG 2 cut(s) 305, 401
Eco24I GRGCYC 3 cut(s) 474, 528, 650
Eco47I GGWCC 2 cut(s) 507, 552
Eco52I CGGCCG 2 cut(s) 305, 401
Eco53kI GAGCTC 2 cut(s) 472, 526
EcoICRI GAGCTC 2 cut(s) 472, 526
EcoO109I RGGNCCY 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 160
EcoT38I GRGCYC 3 cut(s) 474, 528, 650
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 2 cut(s) 58, 665
FaiI YATR 4 cut(s) 56, 284, 313, 663
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 4 cut(s) 449, 467, 590, 815
FatI CATG 2 cut(s) 54, 661
Fnu4HI GCNGC 9 cut(s) 83, 130, 202, 381, 401, 404, 642, 672, 688
FokI GGATG 2 cut(s) 689, 867
FriOI GRGCYC 3 cut(s) 474, 528, 650
Fsp4HI GCNGC 9 cut(s) 83, 130, 202, 381, 401, 404, 642, 672, 688
FspI TGCGCA 1 cut(s) 759
GlaI GCGC 2 cut(s) 81, 759
GluI GCNGC 9 cut(s) 83, 130, 202, 381, 401, 404, 642, 672, 688
HaeIII GGCC 3 cut(s) 297, 307, 403
HgaI GACGC 4 cut(s) 158, 356, 444, 620
HhaI GCGC 2 cut(s) 82, 760
Hin1I GRCGYC 1 cut(s) 455
Hin1II CATG 2 cut(s) 58, 665
Hin6I GCGC 2 cut(s) 80, 758
HinP1I GCGC 2 cut(s) 80, 758
HinfI GANTC 5 cut(s) 41, 461, 570, 665, 697
Hpy166II GTNNAC 3 cut(s) 148, 193, 859
Hpy188I TCNGA 3 cut(s) 318, 460, 466
Hpy188III TCNNGA 4 cut(s) 289, 369, 529, 589
Hpy8I GTNNAC 3 cut(s) 148, 193, 859
Hpy99I CGWCG 9 cut(s) 227, 230, 260, 350, 359, 448, 451, 454, 605
HpyAV CCTTC 3 cut(s) 482, 721, 787
HpyCH4III ACNGT 5 cut(s) 50, 197, 500, 709, 748
HpyCH4IV ACGT 3 cut(s) 494, 774, 876
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 523, 766
HpyF3I CTNAG 3 cut(s) 153, 633, 732
HpySE526I ACGT 3 cut(s) 494, 774, 876
Hsp92I GRCGYC 1 cut(s) 455
Hsp92II CATG 2 cut(s) 58, 665
HspAI GCGC 2 cut(s) 80, 758
Kpn2I TCCGGA 2 cut(s) 528, 588
KspI CCGCGG 2 cut(s) 276, 642
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 2 cut(s) 425, 677
LmnI GCTCC 7 cut(s) 107, 114, 377, 523, 531, 591, 591
Lsp1109I GCAGC 2 cut(s) 94, 699
LweI GCATC 1 cut(s) 352
MaeII ACGT 3 cut(s) 494, 774, 876
MalI GATC 1 cut(s) 333
MbiI CCGCTC 1 cut(s) 380
MboI GATC 1 cut(s) 331
MboII GAAGA 8 cut(s) 182, 331, 412, 415, 694, 790, 802, 838
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 3 cut(s) 474, 528, 650
MluCI AATT 3 cut(s) 94, 140, 541
MlyI GAGTC 2 cut(s) 470, 579
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 6 cut(s) 271, 347, 400, 421, 565, 790
MroI TCCGGA 2 cut(s) 528, 588
MroXI GAANNNNTTC 2 cut(s) 62, 419
MseI TTAA 3 cut(s) 627, 693, 719
MslI CAYNNNNRTG 1 cut(s) 854
MspA1I CMGCKG 2 cut(s) 275, 641
MspR9I CCNGG 3 cut(s) 162, 485, 511
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 3 cut(s) 80, 275, 641
MwoI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 523, 766
NciI CCSGG 2 cut(s) 485, 511
NdeII GATC 1 cut(s) 331
NlaIII CATG 2 cut(s) 58, 665
NlaIV GGNNCC 3 cut(s) 52, 124, 553
NmeAIII GCCGAG 2 cut(s) 57, 681
NotI GCGGCCGC 1 cut(s) 401
NsbI TGCGCA 1 cut(s) 759
NspV TTCGAA 3 cut(s) 39, 66, 138
OliI CACNNNNGTG 1 cut(s) 854
PciSI GCTCTTC 2 cut(s) 425, 677
PcsI WCGNNNNNNNCGW 5 cut(s) 201, 446, 452, 554, 821
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 2 cut(s) 62, 419
PfeI GAWTC 3 cut(s) 41, 665, 697
PfoI TCCNGGA 2 cut(s) 483, 509
PinAI ACCGGT 1 cut(s) 832
PkrI GCNGC 9 cut(s) 84, 131, 203, 382, 402, 405, 643, 673, 689
PleI GAGTC 2 cut(s) 469, 578
PpsI GAGTC 2 cut(s) 469, 578
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 552
Psp124BI GAGCTC 2 cut(s) 474, 528
Psp5II RGGWCCY 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 3 cut(s) 52, 124, 553
PspPI GGNCC 2 cut(s) 507, 552
PspPPI RGGWCCY 1 cut(s) 552
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 3 cut(s) 77, 497, 818
RsaNI GTAC 3 cut(s) 76, 496, 817
RseI CAYNNNNRTG 1 cut(s) 854
SacI GAGCTC 2 cut(s) 474, 528
SacII CCGCGG 2 cut(s) 276, 642
SapI GCTCTTC 2 cut(s) 425, 677
SaqAI TTAA 3 cut(s) 627, 693, 719
SatI GCNGC 9 cut(s) 83, 130, 202, 381, 401, 404, 642, 672, 688
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 2 cut(s) 507, 552
SchI GAGTC 2 cut(s) 470, 579
ScrFI CCNGG 3 cut(s) 162, 485, 511
SduI GDGCHC 3 cut(s) 474, 528, 650
SfaNI GCATC 1 cut(s) 352
Sfr303I CCGCGG 2 cut(s) 276, 642
SfuI TTCGAA 3 cut(s) 39, 66, 138
SgrBI CCGCGG 2 cut(s) 276, 642
SinI GGWCC 2 cut(s) 507, 552
SmiMI CAYNNNNRTG 1 cut(s) 854
Sse9I AATT 3 cut(s) 94, 140, 541
SstI GAGCTC 2 cut(s) 474, 528
StyD4I CCNGG 3 cut(s) 160, 483, 509
TaaI ACNGT 5 cut(s) 50, 197, 500, 709, 748
TaiI ACGT 3 cut(s) 497, 777, 879
TaqI TCGA 9 cut(s) 39, 66, 72, 138, 426, 474, 557, 741, 829
TasI AATT 3 cut(s) 94, 140, 541
TauI GCSGC 7 cut(s) 132, 204, 383, 403, 406, 644, 674
TfiI GAWTC 3 cut(s) 41, 665, 697
Tru1I TTAA 3 cut(s) 627, 693, 719
Tru9I TTAA 3 cut(s) 627, 693, 719
TseI GCWGC 2 cut(s) 82, 687
TspDTI ATGAA 3 cut(s) 71, 428, 678
TspGWI ACGGA 4 cut(s) 4, 618, 873, 876
VpaK11BI GGWCC 2 cut(s) 507, 552
XapI RAATTY 2 cut(s) 94, 140
XmnI GAANNNNTTC 2 cut(s) 62, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.