pycom13g08060

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012761
Physical Location & Seq
Reverse (-)
5362840 .. 5363742
903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08060.1

Sequence Viewer

Length: 903 bp
ATGCAAGCGGTTTCCGTGTTTCCCGTGTCGCCGAGCTTCGAATCCCACGCTTCCATGAACGATTTCGAAGTCGAGTACGCGCAGCTCTCTGCGAATTTCAGCTCCAAGCTTCAAATCGCCGGTTCCGGTGGCGAATTCGAATTCGTGGACGCTGAGAAGCCAGGAATGGAAGATAAAGAAGCTCAAATCGTGGACGGTGGCGGCGAATGCGAGAGCGAAAACGACGACGATTTCTCTTTCGCCCCCACGAACGCCGACGGGTCGCCGATTTGCGCGGAGGATATATTCCAGAACGGCCACATCCGGCCGGTATATCCGATTTTCAACCGAGATCTCCTCTTCGCCGACGCTGACGACGGCGATGCTTCAAGAGCCGGAGCGGCTTCGTCCTCGTCTCTGCGGTCGCCATTGATGAAGCTCTTCTTCGAGGAACGGGACACGCCGTCGTCGTCGGCGTCCGAGTCGGACGAGCTCGAAGCAGTCCCGGAAGGAACGTACTGTGAGTGGTCCGGGAAGCCGGTGGAGGCGGCGGTGGAGCTCCGGAACAAGAGCAATTCCACGGGGTCGTCGAAGCTGTGGAGAGTCAGGGACTTGAAGCTCCGGTGCAACAGCGACGGAAAGGACGCATTTGTTTTCCTTAAACCTAAGTCCGTGGCGAGCCCCAAGCCGAGCCATGAATCGGCGGCGGATGAGAAGAGCAGCGGTAAGATTCAAAAGACGGTGGAGAAGTTAAAAGGGAAGGCTAAGAAAGTCGAAACGGTGTCGTCTGCGCACGAGAAGCATTACGTGAAGAACAGAGAGAAGAAGGAGGGGGACAAACGGCGGTCGTACTTGCCGTATCGGCCGGTGGTTGGGTTCTTCACAAATGTGAACGGATTGAGCAAAAACGTCCATCCGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

301

Amino Acids

32.85

Weight (kDa)

5.26

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 279 5.6e-30 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 771
AccBSI CCGCTC 1 cut(s) 380
AccII CGCG 2 cut(s) 80, 275
AccIII TCCGGA 1 cut(s) 540
AcoI YGGCCR 3 cut(s) 295, 305, 842
AcsI RAATTY 3 cut(s) 94, 134, 140
AcyI GRCGYC 1 cut(s) 455
AfaI GTAC 3 cut(s) 77, 497, 830
AfiI CCNNNNNNNGG 2 cut(s) 679, 851
AgsI TTSAA 5 cut(s) 113, 325, 369, 595, 713
AhdI GACNNNNNGTC 1 cut(s) 442
AjnI CCWGG 1 cut(s) 160
AleI CACNNNNGTG 1 cut(s) 866
Alw21I GWGCWC 2 cut(s) 474, 540
Alw26I GTCTC 1 cut(s) 399
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 3 cut(s) 295, 305, 842
ApeKI GCWGC 2 cut(s) 82, 699
ApoI RAATTY 3 cut(s) 94, 134, 140
Asp700I GAANNNNTTC 2 cut(s) 62, 419
AspLEI GCGC 3 cut(s) 82, 275, 772
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 2 cut(s) 485, 511
AsuII TTCGAA 3 cut(s) 39, 66, 138
AvaII GGWCC 1 cut(s) 507
BanII GRGCYC 3 cut(s) 474, 540, 662
BauI CACGAG 1 cut(s) 773
Bbv12I GWGCWC 2 cut(s) 474, 540
BbvI GCAGC 2 cut(s) 94, 711
BccI CCATC 1 cut(s) 900
BceAI ACGGC 5 cut(s) 310, 373, 427, 820, 836
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 162
BcnI CCSGG 2 cut(s) 485, 511
BcoDI GTCTC 1 cut(s) 399
BglI GCCNNNNNGGC 2 cut(s) 380, 841
BglII AGATCT 1 cut(s) 331
BisI GCNGC 6 cut(s) 83, 202, 381, 528, 684, 700
BlsI GCNGC 6 cut(s) 84, 203, 382, 529, 685, 701
Bme1390I CCNGG 3 cut(s) 162, 485, 511
Bme18I GGWCC 1 cut(s) 507
BmeRI GACNNNNNGTC 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 3 cut(s) 162, 485, 511
BmsI GCATC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 3 cut(s) 39, 66, 138
BpuMI CCSGG 2 cut(s) 485, 511
BsaAI YACGTR 1 cut(s) 787
BsaHI GRCGYC 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 558, 651
BsaWI WCCGGW 3 cut(s) 125, 540, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 679, 851
Bse118I RCCGGY 4 cut(s) 119, 307, 517, 844
BseAI TCCGGA 1 cut(s) 540
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 558, 651
BseGI GGATG 3 cut(s) 300, 694, 892
BseLI CCNNNNNNNGG 2 cut(s) 679, 851
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 326
BseX3I CGGCCG 2 cut(s) 305, 842
BseXI GCAGC 2 cut(s) 94, 711
Bsh1236I CGCG 2 cut(s) 80, 275
Bsh1285I CGRYCG 4 cut(s) 308, 404, 827, 845
BshFI GGCC 3 cut(s) 297, 307, 844
BsiEI CGRYCG 4 cut(s) 308, 404, 827, 845
BsiHKAI GWGCWC 2 cut(s) 474, 540
BslFI GGGAC 4 cut(s) 449, 467, 602, 827
BslI CCNNNNNNNGG 2 cut(s) 679, 851
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 4 cut(s) 449, 467, 602, 827
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 3 cut(s) 297, 307, 844
Bsp119I TTCGAA 3 cut(s) 39, 66, 138
Bsp1286I GDGCHC 3 cut(s) 474, 540, 662
Bsp13I TCCGGA 1 cut(s) 540
Bsp143I GATC 1 cut(s) 331
BspANI GGCC 3 cut(s) 297, 307, 844
BspCNI CTCAG 1 cut(s) 145
BspEI TCCGGA 1 cut(s) 540
BspFNI CGCG 2 cut(s) 80, 275
BspLI GGNNCC 1 cut(s) 124
BspQI GCTCTTC 2 cut(s) 425, 689
BspT104I TTCGAA 3 cut(s) 39, 66, 138
BsrBI CCGCTC 1 cut(s) 380
BsrFI RCCGGY 4 cut(s) 119, 307, 517, 844
BssAI RCCGGY 4 cut(s) 119, 307, 517, 844
BssECI CCNNGG 2 cut(s) 558, 651
BssMI GATC 1 cut(s) 331
BssNI GRCGYC 1 cut(s) 455
BssSI CACGAG 1 cut(s) 773
Bst2BI CACGAG 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 4 cut(s) 197, 500, 721, 760
Bst6I CTCTTC 3 cut(s) 344, 425, 689
BstACI GRCGYC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 787
BstBI TTCGAA 3 cut(s) 39, 66, 138
BstC8I GCNNGC 2 cut(s) 6, 658
BstDEI CTNAG 3 cut(s) 153, 645, 744
BstDSI CCRYGG 2 cut(s) 558, 651
BstF5I GGATG 3 cut(s) 300, 694, 892
BstFNI CGCG 2 cut(s) 80, 275
BstHHI GCGC 3 cut(s) 82, 275, 772
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 1 cut(s) 331
BstMCI CGRYCG 4 cut(s) 308, 404, 827, 845
BstMWI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 778, 841
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 3 cut(s) 160, 483, 509
BstUI CGCG 2 cut(s) 80, 275
BstV1I GCAGC 2 cut(s) 94, 711
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZI CGGCCG 2 cut(s) 305, 842
BsuRI GGCC 3 cut(s) 297, 307, 844
BtgI CCRYGG 2 cut(s) 558, 651
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 3 cut(s) 300, 694, 892
Cac8I GCNNGC 2 cut(s) 6, 658
CfoI GCGC 3 cut(s) 82, 275, 772
Cfr10I RCCGGY 4 cut(s) 119, 307, 517, 844
Cfr13I GGNCC 1 cut(s) 507
CseI GACGC 4 cut(s) 158, 356, 444, 632
Csp6I GTAC 3 cut(s) 76, 496, 829
CviAII CATG 2 cut(s) 55, 674
CviQI GTAC 3 cut(s) 76, 496, 829
DdeI CTNAG 3 cut(s) 153, 645, 744
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 3 cut(s) 295, 305, 842
EagI CGGCCG 2 cut(s) 305, 842
Eam1104I CTCTTC 3 cut(s) 344, 425, 689
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 3 cut(s) 344, 425, 689
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 2 cut(s) 472, 538
EclXI CGGCCG 2 cut(s) 305, 842
Eco24I GRGCYC 3 cut(s) 474, 540, 662
Eco47I GGWCC 1 cut(s) 507
Eco52I CGGCCG 2 cut(s) 305, 842
Eco53kI GAGCTC 2 cut(s) 472, 538
EcoICRI GAGCTC 2 cut(s) 472, 538
EcoRI GAATTC 2 cut(s) 134, 140
EcoRII CCWGG 1 cut(s) 160
EcoT38I GRGCYC 3 cut(s) 474, 540, 662
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 2 cut(s) 58, 677
FaiI YATR 4 cut(s) 56, 284, 313, 675
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 4 cut(s) 449, 467, 602, 827
FatI CATG 2 cut(s) 54, 673
Fnu4HI GCNGC 6 cut(s) 83, 202, 381, 528, 684, 700
FokI GGATG 3 cut(s) 287, 701, 879
FriOI GRGCYC 3 cut(s) 474, 540, 662
Fsp4HI GCNGC 6 cut(s) 83, 202, 381, 528, 684, 700
FspI TGCGCA 1 cut(s) 771
GlaI GCGC 3 cut(s) 81, 274, 771
GluI GCNGC 6 cut(s) 83, 202, 381, 528, 684, 700
HaeIII GGCC 3 cut(s) 297, 307, 844
HgaI GACGC 4 cut(s) 158, 356, 444, 632
HhaI GCGC 3 cut(s) 82, 275, 772
Hin1I GRCGYC 1 cut(s) 455
Hin1II CATG 2 cut(s) 58, 677
Hin6I GCGC 3 cut(s) 80, 273, 770
HinP1I GCGC 3 cut(s) 80, 273, 770
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 5 cut(s) 41, 461, 582, 677, 709
Hpy166II GTNNAC 3 cut(s) 148, 193, 871
Hpy188I TCNGA 3 cut(s) 318, 460, 466
Hpy188III TCNNGA 3 cut(s) 289, 369, 541
Hpy8I GTNNAC 3 cut(s) 148, 193, 871
HpyAV CCTTC 3 cut(s) 482, 733, 799
HpyCH4III ACNGT 4 cut(s) 197, 500, 721, 760
HpyCH4IV ACGT 3 cut(s) 494, 786, 888
HpyCH4V TGCA 2 cut(s) 4, 606
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 778, 841
HpyF3I CTNAG 3 cut(s) 153, 645, 744
HpySE526I ACGT 3 cut(s) 494, 786, 888
Hsp92I GRCGYC 1 cut(s) 455
Hsp92II CATG 2 cut(s) 58, 677
HspAI GCGC 3 cut(s) 80, 273, 770
Kpn2I TCCGGA 1 cut(s) 540
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 2 cut(s) 425, 689
LmnI GCTCC 5 cut(s) 107, 377, 535, 543, 603
Lsp1109I GCAGC 2 cut(s) 94, 711
LweI GCATC 1 cut(s) 352
MaeII ACGT 3 cut(s) 494, 786, 888
MalI GATC 1 cut(s) 333
MbiI CCGCTC 1 cut(s) 380
MboI GATC 1 cut(s) 331
MboII GAAGA 8 cut(s) 182, 331, 412, 415, 706, 802, 814, 850
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 3 cut(s) 474, 540, 662
MluCI AATT 4 cut(s) 94, 134, 140, 553
MlyI GAGTC 2 cut(s) 470, 591
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 6 cut(s) 271, 347, 400, 421, 517, 802
MroI TCCGGA 1 cut(s) 540
MroXI GAANNNNTTC 2 cut(s) 62, 419
MseI TTAA 3 cut(s) 639, 731, 901
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 702
MspR9I CCNGG 3 cut(s) 162, 485, 511
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 2 cut(s) 80, 275
MwoI GCNNNNNNNGC 5 cut(s) 207, 371, 380, 778, 841
NciI CCSGG 2 cut(s) 485, 511
NdeII GATC 1 cut(s) 331
NlaIII CATG 2 cut(s) 58, 677
NlaIV GGNNCC 1 cut(s) 124
NmeAIII GCCGAG 2 cut(s) 57, 693
NsbI TGCGCA 1 cut(s) 771
NspV TTCGAA 3 cut(s) 39, 66, 138
OliI CACNNNNGTG 1 cut(s) 866
PciSI GCTCTTC 2 cut(s) 425, 689
PcsI WCGNNNNNNNCGW 5 cut(s) 201, 446, 452, 566, 833
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 2 cut(s) 62, 419
PfeI GAWTC 3 cut(s) 41, 677, 709
PfoI TCCNGGA 2 cut(s) 483, 509
PkrI GCNGC 6 cut(s) 84, 203, 382, 529, 685, 701
PleI GAGTC 2 cut(s) 469, 590
PpsI GAGTC 2 cut(s) 469, 590
Ppu21I YACGTR 1 cut(s) 787
Psp124BI GAGCTC 2 cut(s) 474, 540
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 507
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 3 cut(s) 77, 497, 830
RsaNI GTAC 3 cut(s) 76, 496, 829
RseI CAYNNNNRTG 1 cut(s) 866
SacI GAGCTC 2 cut(s) 474, 540
SapI GCTCTTC 2 cut(s) 425, 689
SaqAI TTAA 3 cut(s) 639, 731, 901
SatI GCNGC 6 cut(s) 83, 202, 381, 528, 684, 700
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 2 cut(s) 470, 591
ScrFI CCNGG 3 cut(s) 162, 485, 511
SduI GDGCHC 3 cut(s) 474, 540, 662
SfaNI GCATC 1 cut(s) 352
SfuI TTCGAA 3 cut(s) 39, 66, 138
SinI GGWCC 1 cut(s) 507
SmiMI CAYNNNNRTG 1 cut(s) 866
Sse9I AATT 4 cut(s) 94, 134, 140, 553
SstI GAGCTC 2 cut(s) 474, 540
StyD4I CCNGG 3 cut(s) 160, 483, 509
TaaI ACNGT 4 cut(s) 197, 500, 721, 760
TaiI ACGT 3 cut(s) 497, 789, 891
TaqI TCGA 8 cut(s) 39, 66, 72, 138, 426, 474, 569, 753
TasI AATT 4 cut(s) 94, 134, 140, 553
TauI GCSGC 4 cut(s) 204, 383, 530, 686
TfiI GAWTC 3 cut(s) 41, 677, 709
Tru1I TTAA 3 cut(s) 639, 731, 901
Tru9I TTAA 3 cut(s) 639, 731, 901
TseI GCWGC 2 cut(s) 82, 699
TspDTI ATGAA 3 cut(s) 71, 428, 690
TspGWI ACGGA 5 cut(s) 4, 630, 640, 885, 888
VpaK11BI GGWCC 1 cut(s) 507
XapI RAATTY 3 cut(s) 94, 134, 140
XmnI GAANNNNTTC 2 cut(s) 62, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.