Rw4G035000

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012761
Physical Location & Seq
Forward (+)
61879612 .. 61880541
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035000.1

Sequence Viewer

Length: 930 bp
ATGCAAGCGGTTTCGGTTCCGCCTTCGTCTCCGAGTTTGGATTCTTACGTCAATTCAAAGAACAATTTCGAATATTCGCAGCTCTCTGCGAATTTCACCGCTGGTGTCAAAGTCTCAGAGGACGTAAAACGCACAGCTTCAGGTGAGGAAGAGAAACCAGAGAAGGAAACGCAAAATGACATCGCTGCTGTAGATGAGGACGAGGTAGAAGTAGAGGAAGAGGAAGAGGAGTTCTCGTTTTTCTGCACGAATCCCGACGGATCACCGATTTCCGCCGACGACGCGTTCCAGGACGGCCAGATCCGGCCGGTGTATCCGATTTTCAACCGGGATCTCCTCTTCGCCGACGCCTACGACGGCGATTCTTCTTTCCGCCAGAAAGAGGCTTCCTCTTCTTCGTCTCTAAGGCCGCCATTGAAAAAGCTCTTCTTCGAAGACCGCGACGCTCCATCGTCGTCGGCCTCCGAATCCGACGAGCTCGAAGCCGTCCCGGAGGGAAGCTACTGCGAGTGGTCCAAGAACGCCAAGGAAGTCTCGCAGGAGCTCCGAAACAACAAGAGCAATTCCACCGGGTCCTCCAAGCTCTGGAGAATCAGGGACTTGGTTCCTCGGAGCAACAGCGACGGCAAGGACGCGTTCGTGTTCCTGAACCCTAATTCGGCGACCCCGAAGCAAAATAGAGCGGCTGCCGCGGCGGCGGCGGTGACCGCGAAGGCCGCCAAGAGCGTAAAGCCGGTGGAGAAAGCTCAGGGGCAAAAGGGTAAAAAGGCCGAAACGGTGTCGTCGGCGCACGAGAAGCATTACGTGATGAACAGAGCGAAGAAGGAAGGGGATAAACGACGGTCGTATTTGCCGTACAGGCCGGGTGTGGTCGGTTTCGGGTTCTTCACCAATGTAAACGGGTTGAGCAGAAATGTTCATCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

309

Amino Acids

34.0

Weight (kDa)

5.22

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 285 1.8e-32 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 585
AccBSI CCGCTC 1 cut(s) 683
AccII CGCG 5 cut(s) 284, 441, 635, 692, 710
AclWI GGATC 3 cut(s) 268, 295, 339
AcoI YGGCCR 2 cut(s) 295, 305
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 3 cut(s) 382, 585, 658
AflIII ACRYGT 2 cut(s) 282, 633
AgsI TTSAA 3 cut(s) 57, 325, 418
AjnI CCWGG 1 cut(s) 288
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 8 cut(s) 82, 137, 424, 478, 501, 544, 583, 746
AluI AGCT 8 cut(s) 82, 137, 424, 478, 501, 544, 583, 746
Alw21I GWGCWC 2 cut(s) 480, 546
Alw26I GTCTC 4 cut(s) 33, 118, 405, 538
AlwI GGATC 3 cut(s) 268, 295, 339
AoxI GGCC 7 cut(s) 295, 305, 407, 459, 714, 768, 860
ApeKI GCWGC 3 cut(s) 79, 185, 686
ApoI RAATTY 1 cut(s) 91
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 790
AspS9I GGNCC 2 cut(s) 513, 573
AsuC2I CCSGG 4 cut(s) 329, 491, 571, 864
AsuHPI GGTGA 5 cut(s) 88, 155, 255, 715, 880
AsuII TTCGAA 2 cut(s) 69, 432
AvaII GGWCC 2 cut(s) 513, 573
BanII GRGCYC 2 cut(s) 480, 546
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 480, 546
BbvI GCAGC 3 cut(s) 91, 172, 673
BccI CCATC 1 cut(s) 457
BceAI ACGGC 5 cut(s) 310, 373, 470, 640, 838
BciT130I CCWGG 1 cut(s) 290
BciVI GTATCC 1 cut(s) 324
BcnI CCSGG 4 cut(s) 329, 491, 571, 864
BcoDI GTCTC 4 cut(s) 33, 118, 405, 538
BfmI CTRYAG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 324
BglI GCCNNNNNGGC 2 cut(s) 695, 859
Bme1390I CCNGG 5 cut(s) 290, 329, 491, 571, 864
Bme18I GGWCC 2 cut(s) 513, 573
BmgT120I GGNCC 2 cut(s) 513, 573
BmiI GGNNCC 3 cut(s) 18, 574, 606
BmrFI CCNGG 5 cut(s) 290, 329, 491, 571, 864
BpiI GAAGAC 1 cut(s) 441
BplI GAGNNNNNCTC 4 cut(s) 218, 250, 374, 406
BpmI CTGGAG 1 cut(s) 607
Bpu10I CCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 2 cut(s) 69, 432
BpuMI CCSGG 4 cut(s) 329, 491, 571, 864
BsaAI YACGTR 1 cut(s) 805
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 525, 608, 690
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 585, 658
Bse118I RCCGGY 2 cut(s) 307, 733
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 3 cut(s) 525, 608, 690
BseGI GGATG 1 cut(s) 919
BseLI CCNNNNNNNGG 3 cut(s) 382, 585, 658
BseMII CTCAG 2 cut(s) 129, 761
BseRI GAGGAG 2 cut(s) 242, 326
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 3 cut(s) 91, 172, 673
BsgI GTGCAG 1 cut(s) 229
Bsh1236I CGCG 5 cut(s) 284, 441, 635, 692, 710
Bsh1285I CGRYCG 2 cut(s) 308, 845
BshFI GGCC 7 cut(s) 297, 307, 409, 461, 716, 770, 862
BsiEI CGRYCG 2 cut(s) 308, 845
BsiHKAI GWGCWC 2 cut(s) 480, 546
BsiSI CCGG 7 cut(s) 304, 308, 328, 491, 570, 734, 863
BslFI GGGAC 2 cut(s) 473, 611
BslI CCNNNNNNNGG 3 cut(s) 382, 585, 658
BsmAI GTCTC 4 cut(s) 33, 118, 405, 538
BsmBI CGTCTC 2 cut(s) 33, 405
BsmFI GGGAC 2 cut(s) 473, 611
BsnI GGCC 7 cut(s) 297, 307, 409, 461, 716, 770, 862
Bsp119I TTCGAA 2 cut(s) 69, 432
Bsp1286I GDGCHC 2 cut(s) 480, 546
Bsp143I GATC 3 cut(s) 260, 300, 331
BspANI GGCC 7 cut(s) 297, 307, 409, 461, 716, 770, 862
BspCNI CTCAG 2 cut(s) 128, 760
BspFNI CGCG 5 cut(s) 284, 441, 635, 692, 710
BspLI GGNNCC 3 cut(s) 18, 574, 606
BspPI GGATC 3 cut(s) 268, 295, 339
BspQI GCTCTTC 1 cut(s) 431
BspT104I TTCGAA 2 cut(s) 69, 432
BsrBI CCGCTC 1 cut(s) 683
BsrFI RCCGGY 2 cut(s) 307, 733
BssAI RCCGGY 2 cut(s) 307, 733
BssECI CCNNGG 3 cut(s) 525, 608, 690
BssMI GATC 3 cut(s) 260, 300, 331
BssNI GRCGYC 1 cut(s) 348
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 778, 843
Bst6I CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
BstACI GRCGYC 1 cut(s) 348
BstBAI YACGTR 1 cut(s) 805
BstBI TTCGAA 2 cut(s) 69, 432
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 115, 404, 747
BstDSI CCRYGG 1 cut(s) 690
BstEII GGTNACC 1 cut(s) 703
BstF5I GGATG 1 cut(s) 919
BstFNI CGCG 5 cut(s) 284, 441, 635, 692, 710
BstHHI GCGC 1 cut(s) 790
BstKTI GATC 3 cut(s) 263, 303, 334
BstMAI GTCTC 4 cut(s) 33, 118, 405, 538
BstMBI GATC 3 cut(s) 260, 300, 331
BstMCI CGRYCG 2 cut(s) 308, 845
BstMWI GCNNNNNNNGC 9 cut(s) 281, 689, 692, 695, 698, 707, 716, 796, 859
BstNI CCWGG 1 cut(s) 290
BstPI GGTNACC 1 cut(s) 703
BstSCI CCNGG 5 cut(s) 288, 327, 489, 569, 862
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 5 cut(s) 284, 441, 635, 692, 710
BstV1I GCAGC 3 cut(s) 91, 172, 673
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 2 cut(s) 300, 331
BstYI RGATCY 2 cut(s) 300, 331
BstZI CGGCCG 1 cut(s) 305
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 7 cut(s) 297, 307, 409, 461, 716, 770, 862
BtgI CCRYGG 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 790
Cfr10I RCCGGY 2 cut(s) 307, 733
Cfr13I GGNCC 2 cut(s) 513, 573
Cfr42I CCGCGG 1 cut(s) 693
CseI GACGC 4 cut(s) 290, 356, 452, 641
Csp6I GTAC 1 cut(s) 856
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 3 cut(s) 115, 404, 747
DpnI GATC 3 cut(s) 262, 302, 333
DpnII GATC 3 cut(s) 260, 300, 331
EaeI YGGCCR 2 cut(s) 295, 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
EarI CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
EciI GGCGGA 3 cut(s) 9, 262, 362
Ecl136II GAGCTC 2 cut(s) 478, 544
EclXI CGGCCG 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 2 cut(s) 480, 546
Eco47I GGWCC 2 cut(s) 513, 573
Eco52I CGGCCG 1 cut(s) 305
Eco53kI GAGCTC 2 cut(s) 478, 544
Eco57I CTGAAG 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 703
EcoICRI GAGCTC 2 cut(s) 478, 544
EcoO109I RGGNCCY 1 cut(s) 573
EcoO65I GGTNACC 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 2 cut(s) 480, 546
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 33, 405
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 2 cut(s) 473, 611
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 2 cut(s) 480, 546
GlaI GCGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 607
HaeIII GGCC 7 cut(s) 297, 307, 409, 461, 716, 770, 862
HapII CCGG 7 cut(s) 304, 308, 328, 491, 570, 734, 863
HgaI GACGC 4 cut(s) 290, 356, 452, 641
HhaI GCGC 1 cut(s) 790
Hin1I GRCGYC 1 cut(s) 348
Hin6I GCGC 1 cut(s) 788
HinP1I GCGC 1 cut(s) 788
HinfI GANTC 5 cut(s) 41, 250, 362, 467, 591
HpaII CCGG 7 cut(s) 304, 308, 328, 491, 570, 734, 863
HphI GGTGA 5 cut(s) 88, 155, 255, 715, 880
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 8 cut(s) 33, 118, 318, 466, 472, 548, 612, 929
Hpy188III TCNNGA 3 cut(s) 254, 586, 646
Hpy8I GTNNAC 1 cut(s) 898
HpyAV CCTTC 5 cut(s) 33, 157, 706, 817, 821
HpyCH4III ACNGT 2 cut(s) 778, 843
HpyCH4IV ACGT 3 cut(s) 48, 123, 804
HpyCH4V TGCA 2 cut(s) 4, 246
HpyF10VI GCNNNNNNNGC 9 cut(s) 281, 689, 692, 695, 698, 707, 716, 796, 859
HpyF3I CTNAG 3 cut(s) 115, 404, 747
HpySE526I ACGT 3 cut(s) 48, 123, 804
Hsp92I GRCGYC 1 cut(s) 348
HspAI GCGC 1 cut(s) 788
KspI CCGCGG 1 cut(s) 693
Kzo9I GATC 3 cut(s) 260, 300, 331
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 4 cut(s) 451, 541, 549, 612
Lsp1109I GCAGC 3 cut(s) 91, 172, 673
MaeII ACGT 3 cut(s) 48, 123, 804
MaeIII GTNAC 1 cut(s) 703
MalI GATC 3 cut(s) 262, 302, 333
MbiI CCGCTC 1 cut(s) 683
MboI GATC 3 cut(s) 260, 300, 331
MflI RGATCY 2 cut(s) 300, 331
MhlI GDGCHC 2 cut(s) 480, 546
MluCI AATT 5 cut(s) 52, 64, 91, 562, 655
MluI ACGCGT 2 cut(s) 282, 633
MmeI TCCRAC 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 101, 692
MspI CCGG 7 cut(s) 304, 308, 328, 491, 570, 734, 863
MspR9I CCNGG 5 cut(s) 290, 329, 491, 571, 864
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 5 cut(s) 284, 441, 635, 692, 710
MwoI GCNNNNNNNGC 9 cut(s) 281, 689, 692, 695, 698, 707, 716, 796, 859
NciI CCSGG 4 cut(s) 329, 491, 571, 864
NdeII GATC 3 cut(s) 260, 300, 331
NlaIV GGNNCC 3 cut(s) 18, 574, 606
NmuCI GTSAC 1 cut(s) 703
NspV TTCGAA 2 cut(s) 69, 432
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 665, 851
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 5 cut(s) 41, 250, 362, 467, 591
PflMI CCANNNNNTGG 1 cut(s) 585
PfoI TCCNGGA 2 cut(s) 288, 489
Ppu21I YACGTR 1 cut(s) 805
PpuMI RGGWCCY 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 480, 546
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 288
PspEI GGTNACC 1 cut(s) 703
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 3 cut(s) 18, 574, 606
PspPI GGNCC 2 cut(s) 513, 573
PspPPI RGGWCCY 1 cut(s) 573
PsuI RGATCY 2 cut(s) 300, 331
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
SacI GAGCTC 2 cut(s) 480, 546
SacII CCGCGG 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 431
Sau3AI GATC 3 cut(s) 260, 300, 331
Sau96I GGNCC 2 cut(s) 513, 573
ScrFI CCNGG 5 cut(s) 290, 329, 491, 571, 864
SduI GDGCHC 2 cut(s) 480, 546
SfcI CTRYAG 1 cut(s) 189
Sfr303I CCGCGG 1 cut(s) 693
SfuI TTCGAA 2 cut(s) 69, 432
SgrBI CCGCGG 1 cut(s) 693
SinI GGWCC 2 cut(s) 513, 573
Sse9I AATT 5 cut(s) 52, 64, 91, 562, 655
SspI AATATT 1 cut(s) 74
SstI GAGCTC 2 cut(s) 480, 546
StyD4I CCNGG 5 cut(s) 288, 327, 489, 569, 862
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 778, 843
TaiI ACGT 3 cut(s) 51, 126, 807
TaqI TCGA 3 cut(s) 69, 432, 480
TasI AATT 5 cut(s) 52, 64, 91, 562, 655
TauI GCSGC 7 cut(s) 412, 686, 692, 695, 698, 701, 719
TfiI GAWTC 5 cut(s) 41, 250, 362, 467, 591
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 3 cut(s) 79, 185, 686
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 2 cut(s) 824, 908
TspGWI ACGGA 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 2 cut(s) 513, 573
XapI RAATTY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.