Prupe.1G253100_v2.0.a1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012761
Physical Location & Seq
Forward (+)
26394152 .. 26395439
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G253100.1

Sequence Viewer

Length: 939 bp
ATGCAAGCGGTTTCGGTGCTGTCTTTGTCTCCCAGCTTCAATTCGTACGATTCGAAGAACGAATTTGAATACGCGCAGCTCTCTGCAAATTTCAGCTCCAAGCTCAAAATCGCTGGTTCCGGTAGCGATTTCGAATTCAATGACACTAAACCCGCAGCTTTAGATAACGAGAAGGAGAAGCCGGAAACGGAAGAGAGCGAAGGTGTTCAGAACGCCGACGCCGGTGGTGATTGCGAGAAGAACGAAGATGAAGACGATTTCTCTTTCGTCTGCACCAACCCCGACGGATCGCCGATTTCCGCCGACGATATATTCCAAAATGGCCAGATCCGTCCGGTGTACCCAATTTTCAACCGGGATCTCCTGTTCGCCGATTCCGACGACGGCGATTCTTCCAGAGCCAGAGGGGCTGCCGCCTCGTCTTCCTCTCTGCGGCCGCCATTGAAGAAGCTCTTCTTCAAAGAGCGGGATACGCCGTCGTCCTCGGCTTCCGAGTCGGACGAGCTCGAAGGAGTCCCCGAAGGAACGTACTGCGAGTGGTCGAGGAAGACCGTGGTAGTTGCGCCGGAGCTCAAGAACAAGAGCAATTCGACGGGTTCGTCGAAGCTGTGGAGAGTCAGAGACTTGAAGCTCAGGAGCAACAGCGACGGGAAAGACGCGTTCGTGTTCCTGAACCCTAATTCGACGACGACGAGCCCAAAGCCGAGCCAGGAAACGGCCGACAACAAGAGCGGCGCTAAGATTGTGAAGACGGTGGAAAAGGTTAAGGGGAAGGTTAAGAAAGCCGAAACGGTGTCGTCGGCGCACGAGAAGCATTACGTGATGAACCGAGCGAAGAAGGAGGGGGATAAACGGCGGTCGTACTTGCCGTATAGGCAGGATCTGGTTGGGTTCTTCACCAATGTGAACGGCTTGAGCAGAAACGTTCATCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

313

Amino Acids

34.36

Weight (kDa)

5.51

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 598
AccBSI CCGCTC 2 cut(s) 466, 732
AccII CGCG 2 cut(s) 74, 659
AciI CCGC 9 cut(s) 8, 153, 300, 414, 433, 437, 466, 732, 856
AclI AACGTT 1 cut(s) 924
AclWI GGATC 4 cut(s) 295, 322, 366, 888
AcoI YGGCCR 3 cut(s) 322, 434, 717
AcsI RAATTY 3 cut(s) 62, 88, 134
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 4 cut(s) 47, 341, 530, 863
AfiI CCNNNNNNNGG 2 cut(s) 432, 715
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 7 cut(s) 40, 68, 139, 352, 445, 460, 628
AjnI CCWGG 1 cut(s) 708
AleI CACNNNNGTG 1 cut(s) 902
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
Alw21I GWGCWC 2 cut(s) 507, 573
Alw26I GTCTC 2 cut(s) 33, 615
AlwI GGATC 4 cut(s) 295, 322, 366, 888
AlwNI CAGNNNCTG 1 cut(s) 883
AoxI GGCC 3 cut(s) 322, 434, 717
ApeKI GCWGC 3 cut(s) 76, 155, 410
ApoI RAATTY 3 cut(s) 62, 88, 134
Asp700I GAANNNNTTC 2 cut(s) 204, 452
AspLEI GCGC 4 cut(s) 76, 565, 737, 805
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 239, 889
AsuII TTCGAA 2 cut(s) 53, 132
BalI TGGCCA 1 cut(s) 324
BanII GRGCYC 3 cut(s) 507, 573, 698
BauI CACGAG 1 cut(s) 806
BbsI GAAGAC 4 cut(s) 258, 414, 554, 755
Bbv12I GWGCWC 2 cut(s) 507, 573
BbvI GCAGC 3 cut(s) 88, 167, 397
BceAI ACGGC 6 cut(s) 400, 460, 732, 853, 869, 925
BciT130I CCWGG 1 cut(s) 710
BciVI GTATCC 1 cut(s) 463
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 33, 615
BfoI RGCGCY 1 cut(s) 738
BfuI GTATCC 1 cut(s) 463
BglI GCCNNNNNGGC 2 cut(s) 407, 874
BisI GCNGC 7 cut(s) 77, 156, 411, 414, 434, 437, 733
BlsI GCNGC 7 cut(s) 78, 157, 412, 415, 435, 438, 734
Bme1390I CCNGG 2 cut(s) 356, 710
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 356, 710
BpiI GAAGAC 4 cut(s) 258, 414, 554, 755
Bpu10I CCTNAGC 1 cut(s) 632
Bpu14I TTCGAA 2 cut(s) 53, 132
BpuEI CTTGAG 2 cut(s) 557, 934
BpuMI CCSGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 820
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 483, 552
BsaWI WCCGGW 2 cut(s) 119, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 432, 715
Bse118I RCCGGY 1 cut(s) 221
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 2 cut(s) 483, 552
BseGI GGATG 1 cut(s) 928
BseLI CCNNNNNNNGG 2 cut(s) 432, 715
BseMII CTCAG 1 cut(s) 646
BseX3I CGGCCG 2 cut(s) 434, 717
BseXI GCAGC 3 cut(s) 88, 167, 397
BseYI CCCAGC 1 cut(s) 32
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 2 cut(s) 74, 659
Bsh1285I CGRYCG 3 cut(s) 437, 720, 860
BshFI GGCC 3 cut(s) 324, 436, 719
BsiEI CGRYCG 3 cut(s) 437, 720, 860
BsiHKAI GWGCWC 2 cut(s) 507, 573
BsiSI CCGG 6 cut(s) 120, 182, 222, 335, 355, 566
BsiWI CGTACG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 432, 715
BsmAI GTCTC 2 cut(s) 33, 615
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 3 cut(s) 324, 436, 719
Bsp119I TTCGAA 2 cut(s) 53, 132
Bsp1286I GDGCHC 3 cut(s) 507, 573, 698
Bsp143I GATC 4 cut(s) 287, 327, 358, 880
BspACI CCGC 9 cut(s) 8, 153, 300, 414, 433, 437, 466, 732, 856
BspANI GGCC 3 cut(s) 324, 436, 719
BspCNI CTCAG 1 cut(s) 645
BspFNI CGCG 2 cut(s) 74, 659
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 4 cut(s) 295, 322, 366, 888
BspQI GCTCTTC 1 cut(s) 458
BspT104I TTCGAA 2 cut(s) 53, 132
BsrBI CCGCTC 2 cut(s) 466, 732
BsrFI RCCGGY 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 483, 552
BssMI GATC 4 cut(s) 287, 327, 358, 880
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 806
Bst2BI CACGAG 1 cut(s) 806
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 553, 754, 793
Bst6I CTCTTC 2 cut(s) 186, 458
BstACI GRCGYC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 820
BstBI TTCGAA 2 cut(s) 53, 132
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 632, 738
BstDSI CCRYGG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 928
BstFNI CGCG 2 cut(s) 74, 659
BstH2I RGCGCY 1 cut(s) 738
BstHHI GCGC 4 cut(s) 76, 565, 737, 805
BstKTI GATC 4 cut(s) 290, 330, 361, 883
BstMAI GTCTC 2 cut(s) 33, 615
BstMBI GATC 4 cut(s) 287, 327, 358, 880
BstMCI CGRYCG 3 cut(s) 437, 720, 860
BstMWI GCNNNNNNNGC 4 cut(s) 407, 472, 811, 874
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 354, 708
BstUI CGCG 2 cut(s) 74, 659
BstV1I GCAGC 3 cut(s) 88, 167, 397
BstV2I GAAGAC 4 cut(s) 258, 414, 554, 755
BstX2I RGATCY 3 cut(s) 327, 358, 880
BstYI RGATCY 3 cut(s) 327, 358, 880
BstZI CGGCCG 2 cut(s) 434, 717
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 3 cut(s) 324, 436, 719
BtgI CCRYGG 1 cut(s) 552
BtsCI GGATG 1 cut(s) 928
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 883
CciNI GCGGCCGC 1 cut(s) 434
CfoI GCGC 4 cut(s) 76, 565, 737, 805
Cfr10I RCCGGY 1 cut(s) 221
CseI GACGC 2 cut(s) 227, 665
Csp6I GTAC 4 cut(s) 46, 340, 529, 862
CviQI GTAC 4 cut(s) 46, 340, 529, 862
DdeI CTNAG 2 cut(s) 632, 738
DpnI GATC 4 cut(s) 289, 329, 360, 882
DpnII GATC 4 cut(s) 287, 327, 358, 880
DrdI GACNNNNNNGTC 1 cut(s) 598
DseDI GACNNNNNNGTC 1 cut(s) 598
EaeI YGGCCR 3 cut(s) 322, 434, 717
EagI CGGCCG 2 cut(s) 434, 717
Eam1104I CTCTTC 2 cut(s) 186, 458
EarI CTCTTC 2 cut(s) 186, 458
EciI GGCGGA 1 cut(s) 289
Ecl136II GAGCTC 2 cut(s) 505, 571
EclXI CGGCCG 2 cut(s) 434, 717
Eco24I GRGCYC 3 cut(s) 507, 573, 698
Eco52I CGGCCG 2 cut(s) 434, 717
Eco53kI GAGCTC 2 cut(s) 505, 571
EcoICRI GAGCTC 2 cut(s) 505, 571
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 708
EcoT38I GRGCYC 3 cut(s) 507, 573, 698
FaiI YATR 2 cut(s) 311, 873
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 440, 472
FaqI GGGAC 1 cut(s) 500
FauI CCCGC 2 cut(s) 160, 459
Fnu4HI GCNGC 7 cut(s) 77, 156, 411, 414, 434, 437, 733
FokI GGATG 1 cut(s) 915
FriOI GRGCYC 3 cut(s) 507, 573, 698
Fsp4HI GCNGC 7 cut(s) 77, 156, 411, 414, 434, 437, 733
GlaI GCGC 4 cut(s) 75, 564, 736, 804
GluI GCNGC 7 cut(s) 77, 156, 411, 414, 434, 437, 733
GsaI CCCAGC 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 738
HaeIII GGCC 3 cut(s) 324, 436, 719
HapII CCGG 6 cut(s) 120, 182, 222, 335, 355, 566
HgaI GACGC 2 cut(s) 227, 665
HhaI GCGC 4 cut(s) 76, 565, 737, 805
Hin1I GRCGYC 1 cut(s) 219
Hin6I GCGC 4 cut(s) 74, 563, 735, 803
HinP1I GCGC 4 cut(s) 74, 563, 735, 803
HinfI GANTC 6 cut(s) 50, 374, 389, 494, 513, 615
HpaII CCGG 6 cut(s) 120, 182, 222, 335, 355, 566
HphI GGTGA 2 cut(s) 239, 889
Hpy166II GTNNAC 2 cut(s) 340, 907
Hpy188I TCNGA 6 cut(s) 210, 379, 493, 499, 620, 938
Hpy188III TCNNGA 4 cut(s) 396, 574, 634, 670
Hpy8I GTNNAC 2 cut(s) 340, 907
HpyAV CCTTC 6 cut(s) 166, 194, 503, 515, 766, 832
HpyCH4III ACNGT 3 cut(s) 553, 754, 793
HpyCH4IV ACGT 3 cut(s) 527, 819, 924
HpyCH4V TGCA 3 cut(s) 4, 86, 273
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 472, 811, 874
HpyF3I CTNAG 2 cut(s) 632, 738
HpySE526I ACGT 3 cut(s) 527, 819, 924
Hsp92I GRCGYC 1 cut(s) 219
HspAI GCGC 4 cut(s) 74, 563, 735, 803
Kzo9I GATC 4 cut(s) 287, 327, 358, 880
LguI GCTCTTC 1 cut(s) 458
LmnI GCTCC 3 cut(s) 101, 568, 636
Lsp1109I GCAGC 3 cut(s) 88, 167, 397
MaeII ACGT 3 cut(s) 527, 819, 924
MalI GATC 4 cut(s) 289, 329, 360, 882
MbiI CCGCTC 2 cut(s) 466, 732
MboI GATC 4 cut(s) 287, 327, 358, 880
MflI RGATCY 3 cut(s) 327, 358, 880
MhlI GDGCHC 3 cut(s) 507, 573, 698
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 7 cut(s) 40, 62, 88, 134, 345, 586, 679
MluI ACGCGT 1 cut(s) 657
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 3 cut(s) 503, 522, 624
MmeI TCCRAC 2 cut(s) 402, 477
MnlI CCTC 6 cut(s) 398, 427, 436, 493, 537, 835
Mox20I TGGCCA 1 cut(s) 324
MroXI GAANNNNTTC 2 cut(s) 204, 452
MscI TGGCCA 1 cut(s) 324
MseI TTAA 2 cut(s) 765, 777
MslI CAYNNNNRTG 1 cut(s) 902
Msp20I TGGCCA 1 cut(s) 324
MspI CCGG 6 cut(s) 120, 182, 222, 335, 355, 566
MspR9I CCNGG 2 cut(s) 356, 710
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 2 cut(s) 74, 659
MwoI GCNNNNNNNGC 4 cut(s) 407, 472, 811, 874
NciI CCSGG 1 cut(s) 356
NdeII GATC 4 cut(s) 287, 327, 358, 880
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 2 cut(s) 464, 729
NotI GCGGCCGC 1 cut(s) 434
NspV TTCGAA 2 cut(s) 53, 132
OliI CACNNNNGTG 1 cut(s) 902
PciSI GCTCTTC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 6 cut(s) 50, 375, 596, 599, 689, 866
PdmI GAANNNNTTC 2 cut(s) 204, 452
PfeI GAWTC 3 cut(s) 50, 374, 389
Pfl23II CGTACG 1 cut(s) 45
PkrI GCNGC 7 cut(s) 78, 157, 412, 415, 435, 438, 734
PleI GAGTC 3 cut(s) 502, 521, 623
PpsI GAGTC 3 cut(s) 502, 521, 623
Ppu21I YACGTR 1 cut(s) 820
Psp124BI GAGCTC 2 cut(s) 507, 573
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 708
PspFI CCCAGC 1 cut(s) 32
PspGI CCWGG 1 cut(s) 708
PspLI CGTACG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 883
PsuI RGATCY 3 cut(s) 327, 358, 880
RsaI GTAC 4 cut(s) 47, 341, 530, 863
RsaNI GTAC 4 cut(s) 46, 340, 529, 862
RseI CAYNNNNRTG 1 cut(s) 902
SacI GAGCTC 2 cut(s) 507, 573
SapI GCTCTTC 1 cut(s) 458
SaqAI TTAA 2 cut(s) 765, 777
SatI GCNGC 7 cut(s) 77, 156, 411, 414, 434, 437, 733
Sau3AI GATC 4 cut(s) 287, 327, 358, 880
SchI GAGTC 3 cut(s) 503, 522, 624
ScrFI CCNGG 2 cut(s) 356, 710
SduI GDGCHC 3 cut(s) 507, 573, 698
SfuI TTCGAA 2 cut(s) 53, 132
SgrAI CRCCGGYG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 902
SmlI CTYRAG 2 cut(s) 572, 913
SmoI CTYRAG 2 cut(s) 572, 913
Sse9I AATT 7 cut(s) 40, 62, 88, 134, 345, 586, 679
SsiI CCGC 9 cut(s) 8, 153, 300, 414, 433, 437, 466, 732, 856
SstI GAGCTC 2 cut(s) 507, 573
StyD4I CCNGG 2 cut(s) 354, 708
TaaI ACNGT 3 cut(s) 553, 754, 793
TaiI ACGT 3 cut(s) 530, 822, 927
TaqI TCGA 7 cut(s) 53, 132, 507, 542, 590, 602, 683
TasI AATT 7 cut(s) 40, 62, 88, 134, 345, 586, 679
TauI GCSGC 4 cut(s) 416, 436, 439, 735
TfiI GAWTC 3 cut(s) 50, 374, 389
Tru1I TTAA 2 cut(s) 765, 777
Tru9I TTAA 2 cut(s) 765, 777
TseI GCWGC 3 cut(s) 76, 155, 410
TspDTI ATGAA 3 cut(s) 264, 839, 917
TspGWI ACGGA 3 cut(s) 203, 300, 320
XapI RAATTY 3 cut(s) 62, 88, 134
XmnI GAANNNNTTC 2 cut(s) 204, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.