Rh4CG432600

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012761
Physical Location & Seq
Forward (+)
67233653 .. 67234576
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG432600.1

Sequence Viewer

Length: 924 bp
ATGCAAGCGATTTCGGTTCCGGCTTCGTCTCCGAGTTTGGATTCTTACGTCAATTCAAAGAACAATTTCGAATATTCGCAGCTCTCTGCGAATTTCACCGCTACTGTCAAAGTCTCAGAGGACGTAAAACGCACAGCTTCAGGTGAGGAAGAGAAACCAGAGAAGGAAACGCAAAATGACGTCGCTGCTGTAGATGAGGACGAGATAGAAGTAGAAGAAGAGGAAGAGGAAGAGGAGTTCTCGTTTTTCTGCACGAATCCCGACGGATCACCGATTTCAGCCGACGACGCGTTCCAGGACGGCCAGATCCGGCCGGTGTATCCGATTTTCAACCGGGATCTCCTCTTCGCCGACGCCTACGACGGCGGTTCTTCTTCCCGCCAGAAAGAGGCTTCCTCTTCTTCGTCTCTAAGGCCGCCATTGAAAAAGCTCTTCTTCGAAGACCGCGACGCTCCATCGTCGTCGGCCTCCGAATCCGACGAGCTCGAAGCCGTCCCGGAGGGAAGCTACTGCGAGTGGTCCAAGAACGCCAAGGAAGTCTCGCCGGAGCTCCGAAACAACAAGAGCAATTCCACCGGGTCCTCCAAGCTCTGGAGAATCAGGGACTTGGTTCCTCGGAGCAACAGCGACGGCAAGGACGCGTTCGTGTTCCTGAACCCTAATTCGGCGACCCCGAAGCAAAATAGAGCGGCTGCGGCGGCGGCGGTGGCCGCTAAGAGCGTAAAACCGGTGGAGAAAGCTCAGGGGCAAAAGGGTAAAAAGGCCGAAACGGTGTCGTCGGCGCACGAGAAGCATTACGTGATGAACAGAGCGAAGAAGGAAGGGGATAAACGACGGTCGTATTTGCCGTACAGGCCGGGTGTGGTCGGTTTCGGGTTCTTCACCAATGTGAACGGGTTGAGCAGAAATGTTCATCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

307

Amino Acids

33.77

Weight (kDa)

5.09

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 94 - 283 1.7e-33 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 591
AccBSI CCGCTC 1 cut(s) 689
AccII CGCG 3 cut(s) 290, 447, 641
AclWI GGATC 3 cut(s) 274, 301, 345
AcoI YGGCCR 3 cut(s) 301, 311, 708
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 2 cut(s) 180, 354
AfaI GTAC 1 cut(s) 851
AfiI CCNNNNNNNGG 3 cut(s) 388, 591, 664
AflIII ACRYGT 2 cut(s) 288, 639
AgeI ACCGGT 1 cut(s) 727
AgsI TTSAA 3 cut(s) 57, 331, 424
AjnI CCWGG 1 cut(s) 294
AleI CACNNNNGTG 1 cut(s) 887
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 8 cut(s) 82, 137, 430, 484, 507, 550, 589, 740
AluI AGCT 8 cut(s) 82, 137, 430, 484, 507, 550, 589, 740
Alw21I GWGCWC 2 cut(s) 486, 552
Alw26I GTCTC 4 cut(s) 33, 118, 411, 544
AlwI GGATC 3 cut(s) 274, 301, 345
AoxI GGCC 7 cut(s) 301, 311, 413, 465, 708, 762, 854
ApeKI GCWGC 3 cut(s) 79, 185, 692
ApoI RAATTY 1 cut(s) 91
AsiGI ACCGGT 1 cut(s) 727
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 2 cut(s) 519, 579
AsuC2I CCSGG 4 cut(s) 335, 497, 577, 858
AsuHPI GGTGA 4 cut(s) 88, 155, 261, 874
AsuII TTCGAA 2 cut(s) 69, 438
AvaII GGWCC 2 cut(s) 519, 579
BanII GRGCYC 2 cut(s) 486, 552
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 486, 552
BbvI GCAGC 3 cut(s) 91, 172, 679
BccI CCATC 1 cut(s) 463
BceAI ACGGC 5 cut(s) 316, 379, 476, 646, 832
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 4 cut(s) 335, 497, 577, 858
BcoDI GTCTC 4 cut(s) 33, 118, 411, 544
BfmI CTRYAG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 853
BisI GCNGC 9 cut(s) 80, 186, 416, 690, 693, 696, 699, 702, 711
BlsI GCNGC 9 cut(s) 81, 187, 417, 691, 694, 697, 700, 703, 712
Bme1390I CCNGG 5 cut(s) 296, 335, 497, 577, 858
Bme18I GGWCC 2 cut(s) 519, 579
BmgT120I GGNCC 2 cut(s) 519, 579
BmiI GGNNCC 3 cut(s) 18, 580, 612
BmrFI CCNGG 5 cut(s) 296, 335, 497, 577, 858
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 4 cut(s) 224, 256, 380, 412
BpmI CTGGAG 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 2 cut(s) 69, 438
BpuMI CCSGG 4 cut(s) 335, 497, 577, 858
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 180, 354
BsaJI CCNNGG 2 cut(s) 531, 614
BsaWI WCCGGW 1 cut(s) 727
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 388, 591, 664
Bse118I RCCGGY 2 cut(s) 313, 727
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 531, 614
BseGI GGATG 1 cut(s) 913
BseLI CCNNNNNNNGG 3 cut(s) 388, 591, 664
BseMII CTCAG 2 cut(s) 129, 755
BseRI GAGGAG 2 cut(s) 248, 332
BseX3I CGGCCG 1 cut(s) 311
BseXI GCAGC 3 cut(s) 91, 172, 679
BsgI GTGCAG 1 cut(s) 235
Bsh1236I CGCG 3 cut(s) 290, 447, 641
Bsh1285I CGRYCG 2 cut(s) 314, 839
BshFI GGCC 7 cut(s) 303, 313, 415, 467, 710, 764, 856
BshTI ACCGGT 1 cut(s) 727
BsiEI CGRYCG 2 cut(s) 314, 839
BsiHKAI GWGCWC 2 cut(s) 486, 552
BsiSI CCGG 9 cut(s) 20, 310, 314, 334, 497, 545, 576, 728, 857
BslFI GGGAC 2 cut(s) 479, 617
BslI CCNNNNNNNGG 3 cut(s) 388, 591, 664
BsmAI GTCTC 4 cut(s) 33, 118, 411, 544
BsmBI CGTCTC 2 cut(s) 33, 411
BsmFI GGGAC 2 cut(s) 479, 617
BsnI GGCC 7 cut(s) 303, 313, 415, 467, 710, 764, 856
Bsp119I TTCGAA 2 cut(s) 69, 438
Bsp1286I GDGCHC 2 cut(s) 486, 552
Bsp143I GATC 3 cut(s) 266, 306, 337
BspANI GGCC 7 cut(s) 303, 313, 415, 467, 710, 764, 856
BspCNI CTCAG 2 cut(s) 128, 754
BspFNI CGCG 3 cut(s) 290, 447, 641
BspLI GGNNCC 3 cut(s) 18, 580, 612
BspPI GGATC 3 cut(s) 274, 301, 345
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 2 cut(s) 69, 438
BsrBI CCGCTC 1 cut(s) 689
BsrFI RCCGGY 2 cut(s) 313, 727
BssAI RCCGGY 2 cut(s) 313, 727
BssECI CCNNGG 2 cut(s) 531, 614
BssMI GATC 3 cut(s) 266, 306, 337
BssNI GRCGYC 2 cut(s) 180, 354
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 1 cut(s) 531
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 3 cut(s) 106, 772, 837
Bst6I CTCTTC 7 cut(s) 144, 213, 219, 225, 350, 403, 437
BstACI GRCGYC 2 cut(s) 180, 354
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 2 cut(s) 69, 438
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 4 cut(s) 115, 410, 714, 741
BstF5I GGATG 1 cut(s) 913
BstFNI CGCG 3 cut(s) 290, 447, 641
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 3 cut(s) 269, 309, 340
BstMAI GTCTC 4 cut(s) 33, 118, 411, 544
BstMBI GATC 3 cut(s) 266, 306, 337
BstMCI CGRYCG 2 cut(s) 314, 839
BstMWI GCNNNNNNNGC 8 cut(s) 287, 695, 698, 701, 707, 710, 790, 853
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 5 cut(s) 294, 333, 495, 575, 856
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 3 cut(s) 290, 447, 641
BstV1I GCAGC 3 cut(s) 91, 172, 679
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 2 cut(s) 306, 337
BstYI RGATCY 2 cut(s) 306, 337
BstZI CGGCCG 1 cut(s) 311
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 7 cut(s) 303, 313, 415, 467, 710, 764, 856
BtsCI GGATG 1 cut(s) 913
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 2 cut(s) 313, 727
Cfr13I GGNCC 2 cut(s) 519, 579
CseI GACGC 4 cut(s) 296, 362, 458, 647
Csp6I GTAC 1 cut(s) 850
CspAI ACCGGT 1 cut(s) 727
CviQI GTAC 1 cut(s) 850
DdeI CTNAG 4 cut(s) 115, 410, 714, 741
DpnI GATC 3 cut(s) 268, 308, 339
DpnII GATC 3 cut(s) 266, 306, 337
EaeI YGGCCR 3 cut(s) 301, 311, 708
EagI CGGCCG 1 cut(s) 311
Eam1104I CTCTTC 7 cut(s) 144, 213, 219, 225, 350, 403, 437
EarI CTCTTC 7 cut(s) 144, 213, 219, 225, 350, 403, 437
Ecl136II GAGCTC 2 cut(s) 484, 550
EclXI CGGCCG 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 2 cut(s) 486, 552
Eco47I GGWCC 2 cut(s) 519, 579
Eco52I CGGCCG 1 cut(s) 311
Eco53kI GAGCTC 2 cut(s) 484, 550
Eco57I CTGAAG 1 cut(s) 123
EcoICRI GAGCTC 2 cut(s) 484, 550
EcoO109I RGGNCCY 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 486, 552
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 2 cut(s) 33, 411
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 479, 617
FauI CCCGC 1 cut(s) 386
Fnu4HI GCNGC 9 cut(s) 80, 186, 416, 690, 693, 696, 699, 702, 711
FokI GGATG 1 cut(s) 900
FriOI GRGCYC 2 cut(s) 486, 552
Fsp4HI GCNGC 9 cut(s) 80, 186, 416, 690, 693, 696, 699, 702, 711
GlaI GCGC 1 cut(s) 783
GluI GCNGC 9 cut(s) 80, 186, 416, 690, 693, 696, 699, 702, 711
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 7 cut(s) 303, 313, 415, 467, 710, 764, 856
HapII CCGG 9 cut(s) 20, 310, 314, 334, 497, 545, 576, 728, 857
HgaI GACGC 4 cut(s) 296, 362, 458, 647
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 2 cut(s) 180, 354
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HinfI GANTC 4 cut(s) 41, 256, 473, 597
HpaII CCGG 9 cut(s) 20, 310, 314, 334, 497, 545, 576, 728, 857
HphI GGTGA 4 cut(s) 88, 155, 261, 874
Hpy166II GTNNAC 1 cut(s) 892
Hpy188I TCNGA 8 cut(s) 33, 118, 324, 472, 478, 554, 618, 923
Hpy188III TCNNGA 3 cut(s) 260, 592, 652
Hpy8I GTNNAC 1 cut(s) 892
HpyAV CCTTC 3 cut(s) 157, 811, 815
HpyCH4III ACNGT 3 cut(s) 106, 772, 837
HpyCH4IV ACGT 4 cut(s) 48, 123, 180, 798
HpyCH4V TGCA 2 cut(s) 4, 252
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 695, 698, 701, 707, 710, 790, 853
HpyF3I CTNAG 4 cut(s) 115, 410, 714, 741
HpySE526I ACGT 4 cut(s) 48, 123, 180, 798
Hsp92I GRCGYC 2 cut(s) 180, 354
HspAI GCGC 1 cut(s) 782
Kzo9I GATC 3 cut(s) 266, 306, 337
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 4 cut(s) 457, 547, 555, 618
Lsp1109I GCAGC 3 cut(s) 91, 172, 679
MaeII ACGT 4 cut(s) 48, 123, 180, 798
MalI GATC 3 cut(s) 268, 308, 339
MbiI CCGCTC 1 cut(s) 689
MboI GATC 3 cut(s) 266, 306, 337
MflI RGATCY 2 cut(s) 306, 337
MhlI GDGCHC 2 cut(s) 486, 552
MluCI AATT 5 cut(s) 52, 64, 91, 568, 661
MluI ACGCGT 2 cut(s) 288, 639
MmeI TCCRAC 1 cut(s) 501
MroXI GAANNNNTTC 1 cut(s) 65
MslI CAYNNNNRTG 1 cut(s) 887
MspI CCGG 9 cut(s) 20, 310, 314, 334, 497, 545, 576, 728, 857
MspR9I CCNGG 5 cut(s) 296, 335, 497, 577, 858
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 3 cut(s) 290, 447, 641
MwoI GCNNNNNNNGC 8 cut(s) 287, 695, 698, 701, 707, 710, 790, 853
NciI CCSGG 4 cut(s) 335, 497, 577, 858
NdeII GATC 3 cut(s) 266, 306, 337
NlaIV GGNNCC 3 cut(s) 18, 580, 612
NspV TTCGAA 2 cut(s) 69, 438
OliI CACNNNNGTG 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 3 cut(s) 444, 671, 845
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 4 cut(s) 41, 256, 473, 597
PflMI CCANNNNNTGG 1 cut(s) 591
PfoI TCCNGGA 2 cut(s) 294, 495
PinAI ACCGGT 1 cut(s) 727
PkrI GCNGC 9 cut(s) 81, 187, 417, 691, 694, 697, 700, 703, 712
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 579
Psp124BI GAGCTC 2 cut(s) 486, 552
Psp5II RGGWCCY 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 18, 580, 612
PspPI GGNCC 2 cut(s) 519, 579
PspPPI RGGWCCY 1 cut(s) 579
PsuI RGATCY 2 cut(s) 306, 337
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
RseI CAYNNNNRTG 1 cut(s) 887
SacI GAGCTC 2 cut(s) 486, 552
SapI GCTCTTC 1 cut(s) 437
SatI GCNGC 9 cut(s) 80, 186, 416, 690, 693, 696, 699, 702, 711
Sau3AI GATC 3 cut(s) 266, 306, 337
Sau96I GGNCC 2 cut(s) 519, 579
ScrFI CCNGG 5 cut(s) 296, 335, 497, 577, 858
SduI GDGCHC 2 cut(s) 486, 552
SfcI CTRYAG 1 cut(s) 189
SfuI TTCGAA 2 cut(s) 69, 438
SinI GGWCC 2 cut(s) 519, 579
SmiMI CAYNNNNRTG 1 cut(s) 887
Sse9I AATT 5 cut(s) 52, 64, 91, 568, 661
SspI AATATT 1 cut(s) 74
SstI GAGCTC 2 cut(s) 486, 552
StyD4I CCNGG 5 cut(s) 294, 333, 495, 575, 856
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 106, 772, 837
TaiI ACGT 4 cut(s) 51, 126, 183, 801
TaqI TCGA 3 cut(s) 69, 438, 486
TasI AATT 5 cut(s) 52, 64, 91, 568, 661
TauI GCSGC 6 cut(s) 418, 692, 698, 701, 704, 713
TfiI GAWTC 4 cut(s) 41, 256, 473, 597
TseI GCWGC 3 cut(s) 79, 185, 692
TspDTI ATGAA 2 cut(s) 818, 902
TspGWI ACGGA 1 cut(s) 279
Van91I CCANNNNNTGG 1 cut(s) 591
VpaK11BI GGWCC 2 cut(s) 519, 579
XapI RAATTY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 65
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.