MD16G1093500.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012761
Physical Location & Seq
Reverse (-)
6463509 .. 6464423
915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1093500.v1.1.491

Sequence Viewer

Length: 915 bp
ATGCAAGCGGTTTCCGTGTTTTCCGTGTCGCCGAGCTTCAATTCCAAGGATTCCGTGAGCGATTTCGAAGCCGAATACGCGCAGCTCTCTGCGAATTTCAGCTCCCAGCTCCAAATCGCCGGTTCCGGCGGCGATTTTGAATTCGAGGACGCTGGGAAGCCAGAAATGGAAGATAAAGAAGCTCAAAGCGTGGACGACGGCGGCGAATGCGAGACCGAAAACGAAGACGATTTCTCTTTCGCCCCCACGAAAGCCGACGGTTCGCCGATTTCTGCGGACGATATATTCCAAAACGGCCAGATCCGGCCGGTATTTCCGTTTTTCAACCGAGATCTTCTCTTCGCCGAAGCCGACGACGGCGATGCTTCCAGAGCCAGAGCGGCCTCGTCTGCAACTTCGTCCTCGTCTCTAAGGCCGCCGTTGAAGAAGCTGTTCTTCGAAGAGCGGGACACGCAGTCGTCGTCGGCGTCCGAGTTGGACGAGCTCCAAGGAGTCCCGGAAGGAACGTACTGCGAGTGGTCAAGGAAGCCGATGGAGGCGGCGCCGGAGCTCCGGAACAAGAGCAATTCCACGGGATCCTCGAAGCTGTGGAGAGTCAGAGACTTGAACCTCCGGAGCAACAGCGACGGCAAAGACGCTTTCGTCTTCCTGAACCCTAAGTCGGCGGCGAGCCAGAAGCCGAGCCAAGGATCGGCGGCGGATGGGAAGAGCAGCTCTGAGATTCAAAAGACGGCGGAGAAGGTAAAGAGGAAGGCTAAGAAAGTCGAAACGGTATCGTCTGCGCACGAGAAACATTACGTGAAGAACAGAGAGAAGAAGGAAGGGGATAAACGGCGGTCGTACTTGCCGTATCGGCCGGTGGTTGGGTTCTTCACCAATGTGAACGTATTGAGCAGAAACGTTCATCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

305

Amino Acids

33.45

Weight (kDa)

5.2

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 283 5.8e-32 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
Acc16I TGCGCA 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 541
AccBSI CCGCTC 2 cut(s) 380, 445
AccII CGCG 1 cut(s) 80
AccIII TCCGGA 2 cut(s) 552, 612
AclI AACGTT 1 cut(s) 900
AclWI GGATC 4 cut(s) 295, 570, 583, 697
AcoI YGGCCR 3 cut(s) 295, 305, 854
AcsI RAATTY 2 cut(s) 94, 140
AcyI GRCGYC 2 cut(s) 467, 542
AfaI GTAC 2 cut(s) 509, 842
AfiI CCNNNNNNNGG 4 cut(s) 661, 686, 691, 863
AgsI TTSAA 6 cut(s) 40, 140, 325, 424, 607, 725
AhdI GACNNNNNGTC 1 cut(s) 454
AleI CACNNNNGTG 1 cut(s) 878
Alw21I GWGCWC 2 cut(s) 486, 552
Alw26I GTCTC 3 cut(s) 206, 411, 594
AlwI GGATC 4 cut(s) 295, 570, 583, 697
Aor13HI TCCGGA 2 cut(s) 552, 612
AoxI GGCC 5 cut(s) 295, 305, 381, 413, 854
ApeKI GCWGC 2 cut(s) 82, 711
ApoI RAATTY 2 cut(s) 94, 140
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 3 cut(s) 82, 544, 784
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 865
AsuII TTCGAA 2 cut(s) 66, 438
BamHI GGATCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 486, 552
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 231, 637
Bbv12I GWGCWC 2 cut(s) 486, 552
BbvI GCAGC 2 cut(s) 94, 723
BccI CCATC 2 cut(s) 526, 695
BceAI ACGGC 8 cut(s) 214, 310, 373, 403, 643, 747, 832, 848
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 3 cut(s) 206, 411, 594
BfoI RGCGCY 1 cut(s) 545
BglI GCCNNNNNGGC 2 cut(s) 380, 853
BglII AGATCT 1 cut(s) 331
BisI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 540, 666, 696, 712
BlsI GCNGC 9 cut(s) 84, 131, 203, 382, 417, 541, 667, 697, 713
Bme1390I CCNGG 1 cut(s) 497
BmeRI GACNNNNNGTC 1 cut(s) 454
BmiI GGNNCC 3 cut(s) 124, 543, 577
BmrFI CCNGG 1 cut(s) 497
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 231, 637
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 2 cut(s) 66, 438
BpuMI CCSGG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 467, 542
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 4 cut(s) 45, 487, 570, 685
BsaWI WCCGGW 2 cut(s) 552, 612
Bsc4I CCNNNNNNNGG 4 cut(s) 661, 686, 691, 863
Bse118I RCCGGY 3 cut(s) 119, 307, 856
BseAI TCCGGA 2 cut(s) 552, 612
BseDI CCNNGG 4 cut(s) 45, 487, 570, 685
BseGI GGATG 2 cut(s) 706, 904
BseLI CCNNNNNNNGG 4 cut(s) 661, 686, 691, 863
BseMII CTCAG 1 cut(s) 708
BseX3I CGGCCG 2 cut(s) 305, 854
BseXI GCAGC 2 cut(s) 94, 723
BseYI CCCAGC 2 cut(s) 105, 152
Bsh1236I CGCG 1 cut(s) 80
Bsh1285I CGRYCG 3 cut(s) 308, 839, 857
BshFI GGCC 5 cut(s) 297, 307, 383, 415, 856
BshNI GGYRCC 1 cut(s) 541
BsiEI CGRYCG 3 cut(s) 308, 839, 857
BsiHKAI GWGCWC 2 cut(s) 486, 552
BsiSI CCGG 9 cut(s) 120, 126, 304, 308, 497, 545, 553, 613, 857
BslFI GGGAC 2 cut(s) 461, 479
BslI CCNNNNNNNGG 4 cut(s) 661, 686, 691, 863
BsmAI GTCTC 3 cut(s) 206, 411, 594
BsmBI CGTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 461, 479
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 5 cut(s) 297, 307, 383, 415, 856
Bso31I GGTCTC 1 cut(s) 206
Bsp119I TTCGAA 2 cut(s) 66, 438
Bsp1286I GDGCHC 2 cut(s) 486, 552
Bsp13I TCCGGA 2 cut(s) 552, 612
Bsp143I GATC 4 cut(s) 300, 331, 575, 689
BspANI GGCC 5 cut(s) 297, 307, 383, 415, 856
BspCNI CTCAG 1 cut(s) 709
BspEI TCCGGA 2 cut(s) 552, 612
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 3 cut(s) 124, 543, 577
BspPI GGATC 4 cut(s) 295, 570, 583, 697
BspQI GCTCTTC 2 cut(s) 435, 701
BspT104I TTCGAA 2 cut(s) 66, 438
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 206
BsrBI CCGCTC 2 cut(s) 380, 445
BsrFI RCCGGY 3 cut(s) 119, 307, 856
BssAI RCCGGY 3 cut(s) 119, 307, 856
BssECI CCNNGG 4 cut(s) 45, 487, 570, 685
BssMI GATC 4 cut(s) 300, 331, 575, 689
BssNI GRCGYC 2 cut(s) 467, 542
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 3 cut(s) 45, 487, 685
Bst2BI CACGAG 1 cut(s) 785
Bst4CI ACNGT 2 cut(s) 260, 772
Bst6I CTCTTC 3 cut(s) 344, 435, 701
BstACI GRCGYC 2 cut(s) 467, 542
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 2 cut(s) 66, 438
BstC8I GCNNGC 2 cut(s) 6, 670
BstDEI CTNAG 4 cut(s) 410, 657, 717, 756
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 706, 904
BstFNI CGCG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 3 cut(s) 82, 544, 784
BstKTI GATC 4 cut(s) 303, 334, 578, 692
BstMAI GTCTC 3 cut(s) 206, 411, 594
BstMBI GATC 4 cut(s) 300, 331, 575, 689
BstMCI CGRYCG 3 cut(s) 308, 839, 857
BstMWI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
BstSCI CCNGG 1 cut(s) 495
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 94, 723
BstV2I GAAGAC 2 cut(s) 231, 637
BstX2I RGATCY 3 cut(s) 300, 331, 575
BstYI RGATCY 3 cut(s) 300, 331, 575
BstZI CGGCCG 2 cut(s) 305, 854
BsuRI GGCC 5 cut(s) 297, 307, 383, 415, 856
BtgI CCRYGG 1 cut(s) 570
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 706, 904
Cac8I GCNNGC 2 cut(s) 6, 670
CfoI GCGC 3 cut(s) 82, 544, 784
Cfr10I RCCGGY 3 cut(s) 119, 307, 856
CseI GACGC 3 cut(s) 158, 456, 644
Csp6I GTAC 2 cut(s) 508, 841
CviQI GTAC 2 cut(s) 508, 841
DdeI CTNAG 4 cut(s) 410, 657, 717, 756
DinI GGCGCC 1 cut(s) 543
DpnI GATC 4 cut(s) 302, 333, 577, 691
DpnII GATC 4 cut(s) 300, 331, 575, 689
DrdI GACNNNNNNGTC 1 cut(s) 641
DriI GACNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 3 cut(s) 295, 305, 854
EagI CGGCCG 2 cut(s) 305, 854
Eam1104I CTCTTC 3 cut(s) 344, 435, 701
Eam1105I GACNNNNNGTC 1 cut(s) 454
EarI CTCTTC 3 cut(s) 344, 435, 701
EciI GGCGGA 2 cut(s) 713, 749
Ecl136II GAGCTC 2 cut(s) 484, 550
EclXI CGGCCG 2 cut(s) 305, 854
Eco130I CCWWGG 3 cut(s) 45, 487, 685
Eco24I GRGCYC 2 cut(s) 486, 552
Eco31I GGTCTC 1 cut(s) 206
Eco52I CGGCCG 2 cut(s) 305, 854
Eco53kI GAGCTC 2 cut(s) 484, 550
EcoICRI GAGCTC 2 cut(s) 484, 550
EcoRI GAATTC 1 cut(s) 140
EcoT14I CCWWGG 3 cut(s) 45, 487, 685
EcoT38I GRGCYC 2 cut(s) 486, 552
EgeI GGCGCC 1 cut(s) 543
EheI GGCGCC 1 cut(s) 543
ErhI CCWWGG 3 cut(s) 45, 487, 685
Esp3I CGTCTC 1 cut(s) 411
FaiI YATR 1 cut(s) 284
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 461, 479
FauI CCCGC 1 cut(s) 438
Fnu4HI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 540, 666, 696, 712
FokI GGATG 2 cut(s) 713, 891
FriOI GRGCYC 2 cut(s) 486, 552
Fsp4HI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 540, 666, 696, 712
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 3 cut(s) 81, 543, 783
GluI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 540, 666, 696, 712
GsaI CCCAGC 2 cut(s) 109, 156
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 5 cut(s) 297, 307, 383, 415, 856
HapII CCGG 9 cut(s) 120, 126, 304, 308, 497, 545, 553, 613, 857
HgaI GACGC 3 cut(s) 158, 456, 644
HhaI GCGC 3 cut(s) 82, 544, 784
Hin1I GRCGYC 2 cut(s) 467, 542
Hin6I GCGC 3 cut(s) 80, 542, 782
HinP1I GCGC 3 cut(s) 80, 542, 782
HinfI GANTC 4 cut(s) 50, 492, 594, 721
HpaII CCGG 9 cut(s) 120, 126, 304, 308, 497, 545, 553, 613, 857
HphI GGTGA 1 cut(s) 865
Hpy166II GTNNAC 2 cut(s) 193, 883
Hpy188I TCNGA 3 cut(s) 472, 599, 718
Hpy188III TCNNGA 4 cut(s) 369, 553, 613, 649
Hpy8I GTNNAC 2 cut(s) 193, 883
Hpy99I CGWCG 7 cut(s) 200, 260, 356, 359, 463, 466, 629
HpyAV CCTTC 5 cut(s) 494, 733, 745, 811, 815
HpyCH4III ACNGT 2 cut(s) 260, 772
HpyCH4IV ACGT 4 cut(s) 506, 798, 885, 900
HpyCH4V TGCA 2 cut(s) 4, 392
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
HpyF3I CTNAG 4 cut(s) 410, 657, 717, 756
HpySE526I ACGT 4 cut(s) 506, 798, 885, 900
Hsp92I GRCGYC 2 cut(s) 467, 542
HspAI GCGC 3 cut(s) 80, 542, 782
KasI GGCGCC 1 cut(s) 541
Kpn2I TCCGGA 2 cut(s) 552, 612
Kzo9I GATC 4 cut(s) 300, 331, 575, 689
LguI GCTCTTC 2 cut(s) 435, 701
LmnI GCTCC 6 cut(s) 107, 114, 489, 547, 555, 615
Lsp1109I GCAGC 2 cut(s) 94, 723
LweI GCATC 1 cut(s) 352
MaeII ACGT 4 cut(s) 506, 798, 885, 900
MalI GATC 4 cut(s) 302, 333, 577, 691
MbiI CCGCTC 2 cut(s) 380, 445
MboI GATC 4 cut(s) 300, 331, 575, 689
MflI RGATCY 3 cut(s) 300, 331, 575
MhlI GDGCHC 2 cut(s) 486, 552
MluCI AATT 4 cut(s) 40, 94, 140, 565
Mly113I GGCGCC 1 cut(s) 542
MlyI GAGTC 2 cut(s) 501, 603
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 7 cut(s) 139, 394, 412, 529, 589, 620, 741
MroI TCCGGA 2 cut(s) 552, 612
MroXI GAANNNNTTC 1 cut(s) 431
MslI CAYNNNNRTG 1 cut(s) 878
MspI CCGG 9 cut(s) 120, 126, 304, 308, 497, 545, 553, 613, 857
MspR9I CCNGG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 212
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
NarI GGCGCC 1 cut(s) 542
NciI CCSGG 1 cut(s) 497
NdeII GATC 4 cut(s) 300, 331, 575, 689
NlaIV GGNNCC 3 cut(s) 124, 543, 577
NmeAIII GCCGAG 2 cut(s) 57, 705
NsbI TGCGCA 1 cut(s) 783
NspV TTCGAA 2 cut(s) 66, 438
OliI CACNNNNGTG 1 cut(s) 878
PciSI GCTCTTC 2 cut(s) 435, 701
PcsI WCGNNNNNNNCGW 6 cut(s) 201, 348, 458, 464, 578, 845
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 50, 721
PfoI TCCNGGA 1 cut(s) 495
PkrI GCNGC 9 cut(s) 84, 131, 203, 382, 417, 541, 667, 697, 713
PleI GAGTC 2 cut(s) 500, 602
PluTI GGCGCC 1 cut(s) 545
PpsI GAGTC 2 cut(s) 500, 602
Ppu21I YACGTR 1 cut(s) 799
Psp124BI GAGCTC 2 cut(s) 486, 552
Psp1406I AACGTT 1 cut(s) 900
PspFI CCCAGC 2 cut(s) 105, 152
PspN4I GGNNCC 3 cut(s) 124, 543, 577
PsuI RGATCY 3 cut(s) 300, 331, 575
RsaI GTAC 2 cut(s) 509, 842
RsaNI GTAC 2 cut(s) 508, 841
RseI CAYNNNNRTG 1 cut(s) 878
SacI GAGCTC 2 cut(s) 486, 552
SapI GCTCTTC 2 cut(s) 435, 701
SatI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 540, 666, 696, 712
Sau3AI GATC 4 cut(s) 300, 331, 575, 689
SchI GAGTC 2 cut(s) 501, 603
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 2 cut(s) 486, 552
SfaNI GCATC 1 cut(s) 352
SfoI GGCGCC 1 cut(s) 543
SfuI TTCGAA 2 cut(s) 66, 438
SmiMI CAYNNNNRTG 1 cut(s) 878
Sse9I AATT 4 cut(s) 40, 94, 140, 565
SspDI GGCGCC 1 cut(s) 541
SstI GAGCTC 2 cut(s) 486, 552
StyD4I CCNGG 1 cut(s) 495
StyI CCWWGG 3 cut(s) 45, 487, 685
TaaI ACNGT 2 cut(s) 260, 772
TaiI ACGT 4 cut(s) 509, 801, 888, 903
TaqI TCGA 5 cut(s) 66, 144, 438, 581, 765
TaqII GACCGA 1 cut(s) 230
TasI AATT 4 cut(s) 40, 94, 140, 565
TauI GCSGC 7 cut(s) 132, 204, 383, 418, 542, 668, 698
TfiI GAWTC 2 cut(s) 50, 721
TseI GCWGC 2 cut(s) 82, 711
TspDTI ATGAA 1 cut(s) 893
TspGWI ACGGA 4 cut(s) 4, 13, 43, 306
XapI RAATTY 2 cut(s) 94, 140
XmnI GAANNNNTTC 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.