Rroxscaffold_5G00384360

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012761
Physical Location & Seq
Forward (+)
63771584 .. 63772507
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384360.1

Sequence Viewer

Length: 924 bp
ATGCAAGCGATTTCGGTTCCGGCTTCGTCTCCGAGTTTGGATTCTTACGTCAATTCAAAGAACAATTTCGAGTACTCGCAGCTCTCTGCGAATTTCACCGCTAGTGTCAAAGTCTCAGAGGACGTAAAACGCACAGCTTCAGGTGAGGAAGAGAAACCAGAGAAGGAAGCTCAAAATGACGTCGCTGCTGTAGATGAGGATGAGATAGAAGTAGAAGAAGAGGAAGAGGAGTTCTCGTTTTTCTGCACGAATCCCGACGGATCACCGATTTCCGCCGACGACGCGTTCCAGGATGGCCAGATCCGGCCGGTTTATCCGATTTTCAACCGGGATCTCCTCTTCGCCGACGCCTACGACGGCGATTCTTCTTCCCGCCAGAAAGAGGCTTCCTCTTCTTCGTCTCTAAGGCCGCCATTGAAAAAGCTCTTCTTCGAAGACCGCGACGCTCCATCGTCGTCGGCCTCCGAATCCGACGAGCTCGAAGCCGTCCCGGAGGGAAGCTACTGCGAGTGGTCCAAGAACGCCAAGGAAGTCTCGCCGGAGCTCCGAAACAACAAGAGCAATTCCACCGGGTCCTCCAAGCTCTGGAGAATCAGGGACTTGGTTCCTCGGAGCAACAGCGACGGCAAGGACGCGTTCGTGTTCCTGAACCCTAATTCGGCGACCCTGAAGCAAAATGGAGCAGCGGCGGCGGCGGCGGCCGCGAAGGCCGCTAAGAGCGTAAAGCCGGTGGAGAAAGCTCGGGGGCAAAAGGGTAAAAAGGCCGAAACGGTGTCGTCGGCGCACGAGAAGCATTACGTGATGAACAGAGCGAAGAAGGAAGGGGATAAACGACGGTCGTATTTGCCGTACAGGCCCGGTGTGGTCGGTTTCGGGTTCTTCACCAATGTGAACGGGTTGAGCAAAAATGTTCATCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

307

Amino Acids

33.62

Weight (kDa)

5.22

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 283 2e-33 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 585
AccII CGCG 4 cut(s) 284, 441, 635, 704
AclWI GGATC 3 cut(s) 268, 295, 339
AcoI YGGCCR 3 cut(s) 295, 305, 699
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 123, 689
AcyI GRCGYC 2 cut(s) 180, 348
AfaI GTAC 2 cut(s) 74, 851
AfiI CCNNNNNNNGG 3 cut(s) 382, 585, 658
AflIII ACRYGT 2 cut(s) 282, 633
AgsI TTSAA 3 cut(s) 57, 325, 418
AjnI CCWGG 1 cut(s) 288
AleI CACNNNNGTG 1 cut(s) 887
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 9 cut(s) 82, 137, 170, 424, 478, 501, 544, 583, 740
AluI AGCT 9 cut(s) 82, 137, 170, 424, 478, 501, 544, 583, 740
Alw21I GWGCWC 2 cut(s) 480, 546
Alw26I GTCTC 4 cut(s) 33, 118, 405, 538
AlwI GGATC 3 cut(s) 268, 295, 339
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 8 cut(s) 295, 305, 407, 459, 699, 708, 762, 854
ApeKI GCWGC 3 cut(s) 79, 185, 683
ApoI RAATTY 1 cut(s) 91
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 3 cut(s) 513, 573, 855
AsuC2I CCSGG 4 cut(s) 329, 491, 571, 858
AsuHPI GGTGA 4 cut(s) 88, 155, 255, 874
AsuII TTCGAA 1 cut(s) 432
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 2 cut(s) 513, 573
BalI TGGCCA 1 cut(s) 297
BanII GRGCYC 2 cut(s) 480, 546
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 480, 546
BbvI GCAGC 3 cut(s) 91, 172, 695
BccI CCATC 2 cut(s) 287, 457
BceAI ACGGC 4 cut(s) 373, 470, 640, 832
BciT130I CCWGG 1 cut(s) 290
BcnI CCSGG 4 cut(s) 329, 491, 571, 858
BcoDI GTCTC 4 cut(s) 33, 118, 405, 538
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 189
BglI GCCNNNNNGGC 2 cut(s) 707, 853
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 5 cut(s) 290, 329, 491, 571, 858
Bme18I GGWCC 2 cut(s) 513, 573
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 3 cut(s) 513, 573, 855
BmiI GGNNCC 3 cut(s) 18, 574, 606
BmrFI CCNGG 5 cut(s) 290, 329, 491, 571, 858
BpiI GAAGAC 1 cut(s) 441
BplI GAGNNNNNCTC 4 cut(s) 218, 250, 374, 406
BpmI CTGGAG 1 cut(s) 607
Bpu14I TTCGAA 1 cut(s) 432
BpuMI CCSGG 4 cut(s) 329, 491, 571, 858
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 180, 348
BsaJI CCNNGG 2 cut(s) 525, 608
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 585, 658
Bse118I RCCGGY 2 cut(s) 307, 727
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 525, 608
BseGI GGATG 3 cut(s) 205, 298, 913
BseLI CCNNNNNNNGG 3 cut(s) 382, 585, 658
BseMII CTCAG 1 cut(s) 129
BseRI GAGGAG 2 cut(s) 242, 326
BseX3I CGGCCG 2 cut(s) 305, 699
BseXI GCAGC 3 cut(s) 91, 172, 695
BsgI GTGCAG 1 cut(s) 229
Bsh1236I CGCG 4 cut(s) 284, 441, 635, 704
Bsh1285I CGRYCG 3 cut(s) 308, 702, 839
BshFI GGCC 8 cut(s) 297, 307, 409, 461, 701, 710, 764, 856
BsiEI CGRYCG 3 cut(s) 308, 702, 839
BsiHKAI GWGCWC 2 cut(s) 480, 546
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 9 cut(s) 20, 304, 308, 328, 491, 539, 570, 728, 858
BslFI GGGAC 2 cut(s) 473, 611
BslI CCNNNNNNNGG 3 cut(s) 382, 585, 658
BsmAI GTCTC 4 cut(s) 33, 118, 405, 538
BsmBI CGTCTC 2 cut(s) 33, 405
BsmFI GGGAC 2 cut(s) 473, 611
BsnI GGCC 8 cut(s) 297, 307, 409, 461, 701, 710, 764, 856
BsoBI CYCGRG 1 cut(s) 741
Bsp119I TTCGAA 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 480, 546
Bsp143I GATC 3 cut(s) 260, 300, 331
BspANI GGCC 8 cut(s) 297, 307, 409, 461, 701, 710, 764, 856
BspCNI CTCAG 1 cut(s) 128
BspFNI CGCG 4 cut(s) 284, 441, 635, 704
BspLI GGNNCC 3 cut(s) 18, 574, 606
BspPI GGATC 3 cut(s) 268, 295, 339
BspQI GCTCTTC 1 cut(s) 431
BspT104I TTCGAA 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 307, 727
BssAI RCCGGY 2 cut(s) 307, 727
BssECI CCNNGG 2 cut(s) 525, 608
BssMI GATC 3 cut(s) 260, 300, 331
BssNI GRCGYC 2 cut(s) 180, 348
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 772, 837
Bst6I CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
BstACI GRCGYC 2 cut(s) 180, 348
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 115, 404, 714
BstF5I GGATG 3 cut(s) 205, 298, 913
BstFNI CGCG 4 cut(s) 284, 441, 635, 704
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 3 cut(s) 263, 303, 334
BstMAI GTCTC 4 cut(s) 33, 118, 405, 538
BstMBI GATC 3 cut(s) 260, 300, 331
BstMCI CGRYCG 3 cut(s) 308, 702, 839
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 5 cut(s) 288, 327, 489, 569, 856
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 4 cut(s) 284, 441, 635, 704
BstV1I GCAGC 3 cut(s) 91, 172, 695
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 2 cut(s) 300, 331
BstYI RGATCY 2 cut(s) 300, 331
BstZI CGGCCG 2 cut(s) 305, 699
BsuRI GGCC 8 cut(s) 297, 307, 409, 461, 701, 710, 764, 856
BtsCI GGATG 3 cut(s) 205, 298, 913
Cac8I GCNNGC 1 cut(s) 6
CciNI GCGGCCGC 1 cut(s) 699
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 2 cut(s) 307, 727
Cfr13I GGNCC 3 cut(s) 513, 573, 855
CseI GACGC 4 cut(s) 290, 356, 452, 641
Csp6I GTAC 2 cut(s) 73, 850
CviQI GTAC 2 cut(s) 73, 850
DdeI CTNAG 3 cut(s) 115, 404, 714
DpnI GATC 3 cut(s) 262, 302, 333
DpnII GATC 3 cut(s) 260, 300, 331
EaeI YGGCCR 3 cut(s) 295, 305, 699
EagI CGGCCG 2 cut(s) 305, 699
Eam1104I CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
EarI CTCTTC 6 cut(s) 144, 213, 219, 344, 397, 431
EciI GGCGGA 1 cut(s) 262
Ecl136II GAGCTC 2 cut(s) 478, 544
EclXI CGGCCG 2 cut(s) 305, 699
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 2 cut(s) 480, 546
Eco47I GGWCC 2 cut(s) 513, 573
Eco52I CGGCCG 2 cut(s) 305, 699
Eco53kI GAGCTC 2 cut(s) 478, 544
Eco57I CTGAAG 2 cut(s) 123, 689
Eco88I CYCGRG 1 cut(s) 741
EcoICRI GAGCTC 2 cut(s) 478, 544
EcoO109I RGGNCCY 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 2 cut(s) 480, 546
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 33, 405
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 2 cut(s) 473, 611
FauI CCCGC 1 cut(s) 380
FokI GGATG 3 cut(s) 212, 305, 900
FriOI GRGCYC 2 cut(s) 480, 546
FspBI CTAG 1 cut(s) 102
GlaI GCGC 1 cut(s) 783
GsuI CTGGAG 1 cut(s) 607
HaeIII GGCC 8 cut(s) 297, 307, 409, 461, 701, 710, 764, 856
HapII CCGG 9 cut(s) 20, 304, 308, 328, 491, 539, 570, 728, 858
HgaI GACGC 4 cut(s) 290, 356, 452, 641
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 2 cut(s) 180, 348
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HinfI GANTC 5 cut(s) 41, 250, 362, 467, 591
HpaII CCGG 9 cut(s) 20, 304, 308, 328, 491, 539, 570, 728, 858
HphI GGTGA 4 cut(s) 88, 155, 255, 874
Hpy166II GTNNAC 1 cut(s) 892
Hpy188I TCNGA 8 cut(s) 33, 118, 318, 466, 472, 548, 612, 923
Hpy188III TCNNGA 3 cut(s) 254, 586, 646
Hpy8I GTNNAC 1 cut(s) 892
HpyAV CCTTC 4 cut(s) 157, 700, 811, 815
HpyCH4III ACNGT 2 cut(s) 772, 837
HpyCH4IV ACGT 4 cut(s) 48, 123, 180, 798
HpyCH4V TGCA 2 cut(s) 4, 246
HpyF3I CTNAG 3 cut(s) 115, 404, 714
HpySE526I ACGT 4 cut(s) 48, 123, 180, 798
Hsp92I GRCGYC 2 cut(s) 180, 348
HspAI GCGC 1 cut(s) 782
Kzo9I GATC 3 cut(s) 260, 300, 331
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 5 cut(s) 451, 541, 549, 612, 680
Lsp1109I GCAGC 3 cut(s) 91, 172, 695
MaeI CTAG 1 cut(s) 102
MaeII ACGT 4 cut(s) 48, 123, 180, 798
MalI GATC 3 cut(s) 262, 302, 333
MboI GATC 3 cut(s) 260, 300, 331
MflI RGATCY 2 cut(s) 300, 331
MhlI GDGCHC 2 cut(s) 480, 546
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 5 cut(s) 52, 64, 91, 562, 655
MluI ACGCGT 2 cut(s) 282, 633
MluNI TGGCCA 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 495
Mox20I TGGCCA 1 cut(s) 297
MroXI GAANNNNTTC 1 cut(s) 65
MscI TGGCCA 1 cut(s) 297
MslI CAYNNNNRTG 1 cut(s) 887
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 9 cut(s) 20, 304, 308, 328, 491, 539, 570, 728, 858
MspR9I CCNGG 5 cut(s) 290, 329, 491, 571, 858
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 4 cut(s) 284, 441, 635, 704
NciI CCSGG 4 cut(s) 329, 491, 571, 858
NdeII GATC 3 cut(s) 260, 300, 331
NlaIV GGNNCC 3 cut(s) 18, 574, 606
NotI GCGGCCGC 1 cut(s) 699
NspV TTCGAA 1 cut(s) 432
OliI CACNNNNGTG 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 438, 845
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 5 cut(s) 41, 250, 362, 467, 591
PflMI CCANNNNNTGG 1 cut(s) 585
PfoI TCCNGGA 2 cut(s) 288, 489
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 480, 546
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 3 cut(s) 18, 574, 606
PspPI GGNCC 3 cut(s) 513, 573, 855
PspPPI RGGWCCY 1 cut(s) 573
PsuI RGATCY 2 cut(s) 300, 331
RsaI GTAC 2 cut(s) 74, 851
RsaNI GTAC 2 cut(s) 73, 850
RseI CAYNNNNRTG 1 cut(s) 887
SacI GAGCTC 2 cut(s) 480, 546
SapI GCTCTTC 1 cut(s) 431
Sau3AI GATC 3 cut(s) 260, 300, 331
Sau96I GGNCC 3 cut(s) 513, 573, 855
ScaI AGTACT 1 cut(s) 74
ScrFI CCNGG 5 cut(s) 290, 329, 491, 571, 858
SduI GDGCHC 2 cut(s) 480, 546
SfcI CTRYAG 1 cut(s) 189
SfiI GGCCNNNNNGGCC 1 cut(s) 707
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 2 cut(s) 513, 573
SmiMI CAYNNNNRTG 1 cut(s) 887
Sse9I AATT 5 cut(s) 52, 64, 91, 562, 655
SspMI CTAG 1 cut(s) 102
SstI GAGCTC 2 cut(s) 480, 546
StyD4I CCNGG 5 cut(s) 288, 327, 489, 569, 856
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 772, 837
TaiI ACGT 4 cut(s) 51, 126, 183, 801
TaqI TCGA 3 cut(s) 69, 432, 480
TasI AATT 5 cut(s) 52, 64, 91, 562, 655
TatI WGTACW 1 cut(s) 72
TauI GCSGC 8 cut(s) 412, 689, 692, 695, 698, 701, 704, 713
TfiI GAWTC 5 cut(s) 41, 250, 362, 467, 591
TseI GCWGC 3 cut(s) 79, 185, 683
TspDTI ATGAA 2 cut(s) 818, 902
TspGWI ACGGA 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 2 cut(s) 513, 573
XapI RAATTY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 1 cut(s) 102
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.