MD13G1099800.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001774
Physical Location & Seq
Forward (+)
7049628 .. 7054513
4886 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1099800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGGAATTCTTTCGCGCAGTGCAGGTGGTAAAAAACCAAATGAGAACATGAGGCTTATTGTGACAACTTTTGTAGGTTTAGCTTTTGGATTCTTGATAGGTGTATCCTTTCCAACATTGTCACTAACAAAGCTGAATCTCTCATCCAGCCTTCTTCCAACTGTTGATCTTTCGTACACTGAGGACAAGAAATCAGACATCTCCAGCCAATCACTTCCAAGTTCTTGGCCTTCTGAAACGAGTAAAAATAGCTCAAGTCAAGATCAGAGTTTGAATGGCAAATCGAGGATTTGGGTACCATCAAATCCTAGAGGTGCAGAAATGCTACCACCAGGAATTGTTGCAGCTGAGTCAGACTTCTACTTGCGCAGATTATGGGGCAAGCCTAGTGAGGACTTGACCATCAAACCAAGGTACCTTGTGACATTCACTGTGGGTTTGAATCAGAAAAAGAATATTGATGCAGCAGTGAAAAAGTTTTCAGAGAACTTTACAATTCTCCTTTTTCATTACGATGGCCAAACAACTGAGTGGGATGACTTAGAGTGGTCTAAGCGTGCTATACATATTAGTGCTCGTAAGCAGACTAAATGGTGGTATGCCAAGCGGTTTTTGCATCCTGACATTGTGGCGCCATACGACTACATATTTATCTGGGATGAAGACCTGGGAGTAGAGCATTTTAATGCAGAAGTATACCTAAGACTAGTGAAGAAGCATGGTTTGGAGATTTCACAGCCTGGTTTGGAACCCAACAAAGGTTTAACATGGCAGATGACAAAGAGGAGGGGCGATCGTGAAGTTCACAAACAAACAGAGGAAAAACCGGGCTGGTGCACTGACCCACTTCTGCCTCCATGTGCAGCCTTTATTGAAATCATGGCTCCAGTATTTTCTCGAAGTGCCTGGCGCTGTGTGTGGCATATGATTCAGAATGACTTGGTCCACGGCTGGGGTCTTGATTTTGCCCTGAGAAAATGTGTAGAGCCTGCGCATGAGAAGATCGGCGTTGTAGATTCTCAGTGGATCGTTCATCAAACGGTTCCTTCATTGGGGAGCCAGGGTGAATCGCAAAATGGAAAGGCGCCATGGCAAGGGGTAAGGGAAAGGTGTAGAAAGGAATGGACGATGTTTCAGATGCGGGTAGCAAATGCAGAAAATGCGTATTTTAAGGCTATCGGTGTAGATCCTTCAAATTCGACAGCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

404

Amino Acids

45.78

Weight (kDa)

8.79

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 101 - 387 1.3e-141 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc16I TGCGCA 2 cut(s) 368, 993
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 2 cut(s) 295, 414
AccB1I GGYRCC 4 cut(s) 295, 414, 631, 1084
AccI GTMKAC 1 cut(s) 696
AccII CGCG 1 cut(s) 16
AciI CCGC 2 cut(s) 607, 1141
AclWI GGATC 2 cut(s) 1034, 1181
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 2 cut(s) 6, 1195
AcyI GRCGYC 2 cut(s) 632, 1085
AfaI GTAC 3 cut(s) 176, 297, 416
AfiI CCNNNNNNNGG 4 cut(s) 758, 952, 1052, 1094
AgsI TTSAA 4 cut(s) 274, 442, 875, 1194
AhlI ACTAGT 1 cut(s) 706
AjnI CCWGG 5 cut(s) 331, 666, 739, 905, 1059
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AluBI AGCT 4 cut(s) 83, 133, 252, 347
AluI AGCT 4 cut(s) 83, 133, 252, 347
Alw21I GWGCWC 2 cut(s) 577, 839
Alw44I GTGCAC 1 cut(s) 835
AlwI GGATC 2 cut(s) 1034, 1181
AoxI GGCC 2 cut(s) 227, 517
ApaLI GTGCAC 1 cut(s) 835
ApeKI GCWGC 3 cut(s) 344, 464, 863
ApoI RAATTY 2 cut(s) 6, 1195
Asp700I GAANNNNTTC 1 cut(s) 10
Asp718I GGTACC 2 cut(s) 295, 414
AspLEI GCGC 6 cut(s) 18, 369, 634, 912, 994, 1087
AspS9I GGNCC 1 cut(s) 943
AsuC2I CCSGG 1 cut(s) 828
AsuHPI GGTGA 1 cut(s) 1076
AvaII GGWCC 1 cut(s) 943
BaeGI GKGCMC 1 cut(s) 839
BaeI ACNNNNGTAYC 2 cut(s) 398, 431
BalI TGGCCA 1 cut(s) 519
BanI GGYRCC 4 cut(s) 295, 414, 631, 1084
BbsI GAAGAC 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 577, 839
BbvI GCAGC 3 cut(s) 356, 476, 875
BccI CCATC 3 cut(s) 307, 410, 509
BceAI ACGGC 1 cut(s) 964
BciT130I CCWGG 5 cut(s) 333, 668, 741, 907, 1061
BciVI GTATCC 1 cut(s) 115
BcnI CCSGG 1 cut(s) 828
BcuI ACTAGT 1 cut(s) 706
BfaI CTAG 3 cut(s) 309, 387, 707
BfoI RGCGCY 3 cut(s) 635, 913, 1088
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 115
BisI GCNGC 3 cut(s) 345, 465, 864
BlsI GCNGC 3 cut(s) 346, 466, 865
Bme1390I CCNGG 6 cut(s) 333, 668, 741, 828, 907, 1061
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 8 cut(s) 297, 416, 633, 750, 885, 1044, 1058, 1086
BmrFI CCNGG 6 cut(s) 333, 668, 741, 828, 907, 1061
BmsI GCATC 3 cut(s) 451, 625, 1128
BpiI GAAGAC 1 cut(s) 669
BpmI CTGGAG 2 cut(s) 187, 870
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 828
BsaHI GRCGYC 2 cut(s) 632, 1085
BsaJI CCNNGG 5 cut(s) 410, 667, 946, 1060, 1088
BsaXI ACNNNNNCTCC 2 cut(s) 1048, 1078
Bsc4I CCNNNNNNNGG 4 cut(s) 758, 952, 1052, 1094
Bse1I ACTGG 1 cut(s) 887
BseBI CCWGG 5 cut(s) 333, 668, 741, 907, 1061
BseDI CCNNGG 5 cut(s) 410, 667, 946, 1060, 1088
BseGI GGATG 4 cut(s) 143, 541, 616, 664
BseLI CCNNNNNNNGG 4 cut(s) 758, 952, 1052, 1094
BseMII CTCAG 5 cut(s) 171, 339, 519, 962, 1034
BseNI ACTGG 1 cut(s) 887
BseRI GAGGAG 1 cut(s) 799
BseSI GKGCMC 1 cut(s) 839
BseXI GCAGC 3 cut(s) 356, 476, 875
BseYI CCCAGC 1 cut(s) 951
BsgI GTGCAG 3 cut(s) 42, 336, 882
Bsh1236I CGCG 1 cut(s) 16
Bsh1285I CGRYCG 1 cut(s) 796
BshFI GGCC 2 cut(s) 229, 519
BshNI GGYRCC 4 cut(s) 295, 414, 631, 1084
BsiEI CGRYCG 1 cut(s) 796
BsiHKAI GWGCWC 2 cut(s) 577, 839
BsiSI CCGG 1 cut(s) 827
BslI CCNNNNNNNGG 4 cut(s) 758, 952, 1052, 1094
BsnI GGCC 2 cut(s) 229, 519
Bsp1286I GDGCHC 2 cut(s) 577, 839
Bsp143I GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
Bsp19I CCATGG 1 cut(s) 1088
BspACI CCGC 2 cut(s) 607, 1141
BspANI GGCC 2 cut(s) 229, 519
BspCNI CTCAG 5 cut(s) 172, 340, 520, 963, 1033
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 8 cut(s) 297, 416, 633, 750, 885, 1044, 1058, 1086
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 2 cut(s) 1034, 1181
BspT107I GGYRCC 4 cut(s) 295, 414, 631, 1084
BsrI ACTGG 1 cut(s) 887
BssECI CCNNGG 5 cut(s) 410, 667, 946, 1060, 1088
BssMI GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
BssNAI GTATAC 1 cut(s) 697
BssNI GRCGYC 2 cut(s) 632, 1085
BssT1I CCWWGG 2 cut(s) 410, 1088
Bst1107I GTATAC 1 cut(s) 697
Bst2UI CCWGG 5 cut(s) 333, 668, 741, 907, 1061
Bst4CI ACNGT 3 cut(s) 163, 433, 1042
BstACI GRCGYC 2 cut(s) 632, 1085
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstC8I GCNNGC 3 cut(s) 383, 558, 990
BstDEI CTNAG 8 cut(s) 180, 348, 528, 541, 552, 701, 971, 1020
BstDSI CCRYGG 2 cut(s) 946, 1088
BstF5I GGATG 4 cut(s) 143, 541, 616, 664
BstFNI CGCG 1 cut(s) 16
BstH2I RGCGCY 3 cut(s) 635, 913, 1088
BstHHI GCGC 6 cut(s) 18, 369, 634, 912, 994, 1087
BstKTI GATC 6 cut(s) 169, 265, 796, 1005, 1029, 1189
BstMBI GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
BstMCI CGRYCG 1 cut(s) 796
BstMWI GCNNNNNNNGC 2 cut(s) 613, 1160
BstNI CCWGG 5 cut(s) 333, 668, 741, 907, 1061
BstSCI CCNGG 6 cut(s) 331, 666, 739, 826, 905, 1059
BstSLI GKGCMC 1 cut(s) 839
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 3 cut(s) 356, 476, 875
BstV2I GAAGAC 1 cut(s) 669
BstX2I RGATCY 1 cut(s) 1186
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 1 cut(s) 1186
BstZ17I GTATAC 1 cut(s) 697
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 2 cut(s) 229, 519
BtgI CCRYGG 2 cut(s) 946, 1088
BtsCI GGATG 4 cut(s) 143, 541, 616, 664
BtsI GCAGTG 2 cut(s) 25, 474
BtsIMutI CAGTG 6 cut(s) 25, 177, 429, 474, 837, 1028
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 383, 558, 990
CfoI GCGC 6 cut(s) 18, 369, 634, 912, 994, 1087
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 175, 296, 415
CviAII CATG 7 cut(s) 49, 719, 768, 858, 880, 995, 1089
CviQI GTAC 3 cut(s) 175, 296, 415
DdeI CTNAG 8 cut(s) 180, 348, 528, 541, 552, 701, 971, 1020
DinI GGCGCC 2 cut(s) 633, 1086
DpnI GATC 6 cut(s) 168, 264, 795, 1004, 1028, 1188
DpnII GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
EaeI YGGCCR 1 cut(s) 517
Eco130I CCWWGG 2 cut(s) 410, 1088
Eco47I GGWCC 1 cut(s) 943
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 5 cut(s) 331, 666, 739, 905, 1059
EcoT14I CCWWGG 2 cut(s) 410, 1088
EgeI GGCGCC 2 cut(s) 633, 1086
EheI GGCGCC 2 cut(s) 633, 1086
ErhI CCWWGG 2 cut(s) 410, 1088
FaeI CATG 7 cut(s) 52, 722, 771, 861, 883, 998, 1092
FatI CATG 7 cut(s) 48, 718, 767, 857, 879, 994, 1088
FauI CCCGC 1 cut(s) 1134
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 3 cut(s) 345, 465, 864
FokI GGATG 4 cut(s) 130, 548, 603, 671
Fsp4HI GCNGC 3 cut(s) 345, 465, 864
FspBI CTAG 3 cut(s) 309, 387, 707
FspI TGCGCA 2 cut(s) 368, 993
GlaI GCGC 6 cut(s) 17, 368, 633, 911, 993, 1086
GluI GCNGC 3 cut(s) 345, 465, 864
GsaI CCCAGC 1 cut(s) 955
GsuI CTGGAG 2 cut(s) 187, 870
HaeII RGCGCY 3 cut(s) 635, 913, 1088
HaeIII GGCC 2 cut(s) 229, 519
HapII CCGG 1 cut(s) 827
HhaI GCGC 6 cut(s) 18, 369, 634, 912, 994, 1087
Hin1I GRCGYC 2 cut(s) 632, 1085
Hin1II CATG 7 cut(s) 52, 722, 771, 861, 883, 998, 1092
Hin6I GCGC 6 cut(s) 16, 367, 632, 910, 992, 1085
HinP1I GCGC 6 cut(s) 16, 367, 632, 910, 992, 1085
HinfI GANTC 7 cut(s) 90, 136, 350, 442, 928, 1016, 1067
HpaII CCGG 1 cut(s) 827
HphI GGTGA 1 cut(s) 1076
Hpy166II GTNNAC 5 cut(s) 177, 697, 805, 837, 946
Hpy188I TCNGA 8 cut(s) 196, 235, 267, 355, 447, 484, 933, 1137
Hpy188III TCNNGA 6 cut(s) 94, 260, 620, 797, 897, 959
Hpy8I GTNNAC 5 cut(s) 177, 697, 805, 837, 946
HpyAV CCTTC 4 cut(s) 161, 240, 1056, 1200
HpyCH4III ACNGT 3 cut(s) 163, 433, 1042
HpyCH4V TGCA 9 cut(s) 23, 317, 344, 464, 616, 689, 837, 863, 1154
HpyF10VI GCNNNNNNNGC 2 cut(s) 613, 1160
HpyF3I CTNAG 8 cut(s) 180, 348, 528, 541, 552, 701, 971, 1020
Hsp92I GRCGYC 2 cut(s) 632, 1085
Hsp92II CATG 7 cut(s) 52, 722, 771, 861, 883, 998, 1092
HspAI GCGC 6 cut(s) 16, 367, 632, 910, 992, 1085
KasI GGCGCC 2 cut(s) 631, 1084
KpnI GGTACC 2 cut(s) 299, 418
Kzo9I GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
LmnI GCTCC 2 cut(s) 889, 1056
Lsp1109I GCAGC 3 cut(s) 356, 476, 875
LweI GCATC 3 cut(s) 451, 625, 1128
MaeI CTAG 3 cut(s) 309, 387, 707
MaeIII GTNAC 3 cut(s) 61, 120, 421
MalI GATC 6 cut(s) 168, 264, 795, 1004, 1028, 1188
MboI GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
MboII GAAGA 4 cut(s) 146, 674, 724, 1012
MflI RGATCY 1 cut(s) 1186
MhlI GDGCHC 2 cut(s) 577, 839
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 4 cut(s) 6, 336, 495, 1195
MluNI TGGCCA 1 cut(s) 519
Mly113I GGCGCC 2 cut(s) 632, 1085
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 137, 182
MnlI CCTC 9 cut(s) 45, 175, 279, 305, 385, 777, 780, 811, 864
Mox20I TGGCCA 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 519
MseI TTAA 3 cut(s) 684, 764, 1170
MslI CAYNNNNRTG 3 cut(s) 513, 570, 684
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 6 cut(s) 333, 668, 741, 828, 907, 1061
MvaI CCWGG 5 cut(s) 333, 668, 741, 907, 1061
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 613, 1160
NarI GGCGCC 2 cut(s) 632, 1085
NciI CCSGG 1 cut(s) 828
NcoI CCATGG 1 cut(s) 1088
NdeI CATATG 1 cut(s) 924
NdeII GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
NlaIII CATG 7 cut(s) 52, 722, 771, 861, 883, 998, 1092
NlaIV GGNNCC 8 cut(s) 297, 416, 633, 750, 885, 1044, 1058, 1086
NmuCI GTSAC 3 cut(s) 61, 120, 421
NsbI TGCGCA 2 cut(s) 368, 993
PaqCI CACCTGC 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 6 cut(s) 90, 136, 442, 928, 1016, 1067
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 3 cut(s) 346, 466, 865
Ple19I CGATCG 1 cut(s) 796
PleI GAGTC 1 cut(s) 358
PluTI GGCGCC 2 cut(s) 635, 1088
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 5 cut(s) 331, 666, 739, 905, 1059
PspFI CCCAGC 1 cut(s) 951
PspGI CCWGG 5 cut(s) 331, 666, 739, 905, 1059
PspN4I GGNNCC 8 cut(s) 297, 416, 633, 750, 885, 1044, 1058, 1086
PspPI GGNCC 1 cut(s) 943
PsuI RGATCY 1 cut(s) 1186
PsyI GACNNNGTC 1 cut(s) 941
PvuI CGATCG 1 cut(s) 796
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 3 cut(s) 176, 297, 416
RsaNI GTAC 3 cut(s) 175, 296, 415
RseI CAYNNNNRTG 3 cut(s) 513, 570, 684
SaqAI TTAA 3 cut(s) 684, 764, 1170
SatI GCNGC 3 cut(s) 345, 465, 864
Sau3AI GATC 6 cut(s) 166, 262, 793, 1002, 1026, 1186
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 6 cut(s) 333, 668, 741, 828, 907, 1061
SduI GDGCHC 2 cut(s) 577, 839
SfaNI GCATC 3 cut(s) 451, 625, 1128
SfoI GGCGCC 2 cut(s) 633, 1086
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 3 cut(s) 513, 570, 684
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
SpeI ACTAGT 1 cut(s) 706
Sse9I AATT 4 cut(s) 6, 336, 495, 1195
SsiI CCGC 2 cut(s) 607, 1141
SspDI GGCGCC 2 cut(s) 631, 1084
SspI AATATT 1 cut(s) 457
SspMI CTAG 3 cut(s) 309, 387, 707
StyD4I CCNGG 6 cut(s) 331, 666, 739, 826, 905, 1059
StyI CCWWGG 2 cut(s) 410, 1088
TaaI ACNGT 3 cut(s) 163, 433, 1042
TaqI TCGA 3 cut(s) 284, 898, 1199
TasI AATT 4 cut(s) 6, 336, 495, 1195
TfiI GAWTC 6 cut(s) 90, 136, 442, 928, 1016, 1067
Tru1I TTAA 3 cut(s) 684, 764, 1170
Tru9I TTAA 3 cut(s) 684, 764, 1170
TscAI CASTG 6 cut(s) 25, 184, 436, 474, 844, 1028
TseFI GTSAC 3 cut(s) 61, 120, 421
TseI GCWGC 3 cut(s) 344, 464, 863
Tsp45I GTSAC 3 cut(s) 61, 120, 421
TspDTI ATGAA 4 cut(s) 497, 675, 1022, 1038
TspRI CASTG 6 cut(s) 25, 184, 436, 474, 844, 1028
Tth111I GACNNNGTC 1 cut(s) 941
VneI GTGCAC 1 cut(s) 835
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 2 cut(s) 6, 1195
XmiI GTMKAC 1 cut(s) 696
XmnI GAANNNNTTC 1 cut(s) 10
XspI CTAG 3 cut(s) 309, 387, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.