Rorug04G0266100

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001774
Physical Location & Seq
Reverse (-)
43823800 .. 43826769
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0266100.1

Sequence Viewer

Length: 1755 bp
ATGTGGAAGCTCTTCAGTTCAATGAGAAAGTGGATCGGAAACTACCTCTACTTTTCCAAGGCTACTTTATGCATCCGGGGTTTGGTCTTGAGCCATAGCTTCGTAAACCATGTGGTAATGTCAGTATTGATAACGTACCTCACGGTTGAAATTTGGAAGCCATCAAATGATAATTATCAGCTGGCTGCTGTAGCTATAAATATGCTAGATGGGGTATCATCCATCATGGTGTGTGTCCTTGCTTTCATCTCAAACAGGGTCGGTCCTTTTAAGGTGATTGCCTCCACGAATGCTGCCTATATTCTTGGCCTGGTTCTATTGTGGAGTTCATTTAATTATGCGGGCATGAAAGCCAATGCATCTGAAGTATTCTACGGAGCTGGCCTACTGTTAGCATTAGGCGAGGCTGGACGGCCGGCTGCTTTGGGTAAATTTCTCGACGACCAATATTTAAGTGAAGCTGATCACAAGATCAAATTACCCGAAACAAAAAGTATTGAAGAAGAAGATCATATTTCGACCAGAAACGAAGAACAAGAAGATCATATTTCGACCAGAAAAGAAGAAGAGGACAAGCAAAAACGAGTGGAAGAGGACAAGCAAAAACGTGGGGAAACCCGTCAAACCGCTTTATGGAGCCATCCCTGGTTCATAGGTGCGGCTGCACCTCTTGCCCTTGTAAATAGATCTTGGAAAACTCAACTACTGCTCTCAATGATCGCGGTGGGAGTTTCTTACTTATTGTTCTGGTATGGCGTCTTCGCGTATTCCAGAAATATCAGAAATGAATCAAATGATCCAGCAGTAGAAGATGCACCAAGAGATCCTGGACTTTGCCAAGCCATCAAAAGTCTCTCACCACCAGAAAAAACAGCAAGCTTGAAGTTTTGGCTCAAACCCAATTCCAGAAAACGTTATTACAAAGAAATGCTAGTCATGTGCTTAGCCTTCATCGTATACAGTCTGGTGGAGGCAACGGGGAGCACCTTCTTTTTCAATCAAATGAGTCTACTGAACTATCACATCGGGAACTTTAAATTGCCCATCATATATTTCGTTATACTCCAATCCTTGTCAAGCTCCGTAATCTCATTTCTCTGGAGGTTACTAGTTCCAAATCCGTCAAAACGTGCTACGTTGGTGAGGATCGGCTGTGGTTTAGTTTGCTCTGTGCTATGTATGGTTGCTGCTTGGCAAGTTGAGATTCGTAGGAAAATTAAGATCAATACGGAATACATAGGTAAACCCAAACCCGACAAAATTTCCATGAGCATTCTTTGGTTAATTCCACAATTCTCCCTGTTAGGGTTTATGAGAGGGTTAGCGGCAGATGGATTGATCGAGTTTTTTGCGGATCGAGTTGATGGTAATGGTAAAAGAAAGGCTAGGTATTATGGATCCTACGCCAGTGAATTAGTCCTGGGTATTGGTACACTACTAACCGCCGTTAGTATTTTGGGGTTTAGACGAACTTGGTTGGCTAATGATATATACAAGAATCGTCTGGATAAGTACTACAGGGGACTAGTATTCATAGGTGTTGCTAACCTTTGCTATTACTTCTGTGTCGCCGTTTGCTTTTATACCAAGGAGGATAACTCGCAAGTAATTTGTGCCGCAGATGGAAATGAACAAAGTGAAATGCCGGGCGAGGACAAAAAATATTATCATCACAAGGTGTCCTCAGGTGGTGCGGAGACTCCGGACTTGGAAGAAATGAAGTCGAGTTTTGATTGTTGTAGTAGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

584

Amino Acids

66.02

Weight (kDa)

8.8

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 308 - 452 1e-08 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 957, 1009
AccII CGCG 2 cut(s) 722, 764
AccIII TCCGGA 1 cut(s) 1702
AciI CCGC 9 cut(s) 341, 627, 659, 722, 1325, 1352, 1443, 1617, 1694
AclI AACGTT 1 cut(s) 913
AclWI GGATC 7 cut(s) 41, 791, 818, 1154, 1362, 1392, 1405
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 3 cut(s) 150, 431, 1260
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 756
AdeI CACNNNGTG 1 cut(s) 1678
AfaI GTAC 3 cut(s) 137, 1432, 1514
AfiI CCNNNNNNNGG 3 cut(s) 82, 633, 1305
AgsI TTSAA 5 cut(s) 21, 149, 500, 883, 997
AhlI ACTAGT 2 cut(s) 1108, 1525
AjnI CCWGG 4 cut(s) 309, 644, 826, 1419
AluBI AGCT 8 cut(s) 10, 99, 181, 194, 380, 461, 879, 1080
AluI AGCT 8 cut(s) 10, 99, 181, 194, 380, 461, 879, 1080
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 2 cut(s) 857, 1691
AlwI GGATC 7 cut(s) 41, 791, 818, 1154, 1362, 1392, 1405
Aor13HI TCCGGA 1 cut(s) 1702
AoxI GGCC 3 cut(s) 307, 382, 413
ApeKI GCWGC 5 cut(s) 185, 293, 419, 662, 1187
ApoI RAATTY 3 cut(s) 150, 431, 1260
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 2 cut(s) 77, 1647
AsuHPI GGTGA 3 cut(s) 286, 849, 1153
AvaII GGWCC 1 cut(s) 263
AxyI CCTNAGG 1 cut(s) 1684
BamHI GGATCC 1 cut(s) 1397
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbsI GAAGAC 1 cut(s) 751
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 5 cut(s) 172, 280, 406, 649, 1174
BccI CCATC 9 cut(s) 169, 203, 230, 648, 851, 1052, 1325, 1358, 1616
BceAI ACGGC 3 cut(s) 428, 1430, 1556
BcgI CGANNNNNNTGC 2 cut(s) 1331, 1365
BciT130I CCWGG 4 cut(s) 311, 646, 828, 1421
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 2 cut(s) 77, 1647
BcoDI GTCTC 2 cut(s) 857, 1691
BcuI ACTAGT 2 cut(s) 1108, 1525
BfaI CTAG 5 cut(s) 206, 932, 1109, 1386, 1526
BfmI CTRYAG 2 cut(s) 189, 1516
BglII AGATCT 1 cut(s) 686
BisI GCNGC 8 cut(s) 186, 294, 420, 660, 663, 1188, 1326, 1617
BlpI GCTNAGC 1 cut(s) 943
BlsI GCNGC 8 cut(s) 187, 295, 421, 661, 664, 1189, 1327, 1618
BmcAI AGTACT 1 cut(s) 1514
Bme1390I CCNGG 6 cut(s) 77, 311, 646, 828, 1421, 1647
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 638, 1399
BmrFI CCNGG 6 cut(s) 77, 311, 646, 828, 1421, 1647
BmsI GCATC 3 cut(s) 81, 368, 802
BpiI GAAGAC 1 cut(s) 751
BplI GAGNNNNNCTC 2 cut(s) 1583, 1615
BpmI CTGGAG 1 cut(s) 1120
Bpu1102I GCTNAGC 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 2 cut(s) 77, 1647
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 756
BsaJI CCNNGG 5 cut(s) 57, 76, 644, 1420, 1587
BsaWI WCCGGW 1 cut(s) 1702
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 633, 1305
Bse118I RCCGGY 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 1407
Bse21I CCTNAGG 1 cut(s) 1684
Bse8I GATNNNNATC 1 cut(s) 174
BseAI TCCGGA 1 cut(s) 1702
BseBI CCWGG 4 cut(s) 311, 646, 828, 1421
BseDI CCNNGG 5 cut(s) 57, 76, 644, 1420, 1587
BseGI GGATG 3 cut(s) 72, 218, 640
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 3 cut(s) 82, 633, 1305
BseMII CTCAG 1 cut(s) 1698
BseNI ACTGG 1 cut(s) 1407
BseX3I CGGCCG 1 cut(s) 413
BseXI GCAGC 5 cut(s) 172, 280, 406, 649, 1174
BsgI GTGCAG 1 cut(s) 648
Bsh1236I CGCG 2 cut(s) 722, 764
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 309, 384, 415
BsiEI CGRYCG 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 986
BsiSI CCGG 4 cut(s) 76, 416, 1646, 1703
BslFI GGGAC 1 cut(s) 1536
BslI CCNNNNNNNGG 3 cut(s) 82, 633, 1305
BsmAI GTCTC 2 cut(s) 857, 1691
BsmFI GGGAC 1 cut(s) 1536
BsmI GAATGC 2 cut(s) 295, 1272
BsnI GGCC 3 cut(s) 309, 384, 415
Bsp1286I GDGCHC 1 cut(s) 986
Bsp13I TCCGGA 1 cut(s) 1702
Bsp1720I GCTNAGC 1 cut(s) 943
BspACI CCGC 9 cut(s) 341, 627, 659, 722, 1325, 1352, 1443, 1617, 1694
BspANI GGCC 3 cut(s) 309, 384, 415
BspCNI CTCAG 1 cut(s) 1697
BspEI TCCGGA 1 cut(s) 1702
BspFNI CGCG 2 cut(s) 722, 764
BspLI GGNNCC 2 cut(s) 638, 1399
BspPI GGATC 7 cut(s) 41, 791, 818, 1154, 1362, 1392, 1405
BspQI GCTCTTC 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 415
BsrI ACTGG 1 cut(s) 1407
BssAI RCCGGY 1 cut(s) 415
BssECI CCNNGG 5 cut(s) 57, 76, 644, 1420, 1587
BssNAI GTATAC 1 cut(s) 958
BssNI GRCGYC 1 cut(s) 756
BssT1I CCWWGG 2 cut(s) 57, 1587
Bst1107I GTATAC 1 cut(s) 958
Bst2UI CCWGG 4 cut(s) 311, 646, 828, 1421
Bst4CI ACNGT 3 cut(s) 145, 390, 962
Bst6I CTCTTC 3 cut(s) 17, 561, 585
BstACI GRCGYC 1 cut(s) 756
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 5 cut(s) 183, 343, 382, 417, 877
BstDEI CTNAG 2 cut(s) 943, 1684
BstF5I GGATG 3 cut(s) 72, 218, 640
BstFNI CGCG 2 cut(s) 722, 764
BstMAI GTCTC 2 cut(s) 857, 1691
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 2 cut(s) 191, 671
BstNI CCWGG 4 cut(s) 311, 646, 828, 1421
BstSCI CCNGG 6 cut(s) 75, 309, 644, 826, 1419, 1645
BstSFI CTRYAG 2 cut(s) 189, 1516
BstUI CGCG 2 cut(s) 722, 764
BstV1I GCAGC 5 cut(s) 172, 280, 406, 649, 1174
BstV2I GAAGAC 1 cut(s) 751
BstX2I RGATCY 3 cut(s) 686, 823, 1397
BstYI RGATCY 3 cut(s) 686, 823, 1397
BstZ17I GTATAC 1 cut(s) 958
BstZI CGGCCG 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 1684
BsuRI GGCC 3 cut(s) 309, 384, 415
BtsCI GGATG 3 cut(s) 72, 218, 640
BtsIMutI CAGTG 1 cut(s) 1414
Cac8I GCNNGC 5 cut(s) 183, 343, 382, 417, 877
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 263
CseI GACGC 1 cut(s) 745
Csp6I GTAC 3 cut(s) 136, 1431, 1513
CviAII CATG 5 cut(s) 110, 226, 346, 937, 1267
CviQI GTAC 3 cut(s) 136, 1431, 1513
DdeI CTNAG 2 cut(s) 943, 1684
DraI TTTAAA 1 cut(s) 1036
DraIII CACNNNGTG 1 cut(s) 1678
EaeI YGGCCR 1 cut(s) 413
EagI CGGCCG 1 cut(s) 413
Eam1104I CTCTTC 3 cut(s) 17, 561, 585
EarI CTCTTC 3 cut(s) 17, 561, 585
EclXI CGGCCG 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 57, 1587
Eco47I GGWCC 1 cut(s) 263
Eco52I CGGCCG 1 cut(s) 413
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 1684
EcoRII CCWGG 4 cut(s) 309, 644, 826, 1419
EcoT14I CCWWGG 2 cut(s) 57, 1587
EcoT22I ATGCAT 2 cut(s) 74, 361
ErhI CCWWGG 2 cut(s) 57, 1587
FaeI CATG 5 cut(s) 113, 229, 349, 940, 1270
FaqI GGGAC 1 cut(s) 1536
FatI CATG 5 cut(s) 109, 225, 345, 936, 1266
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 1 cut(s) 463
FblI GTMKAC 2 cut(s) 957, 1009
Fnu4HI GCNGC 8 cut(s) 186, 294, 420, 660, 663, 1188, 1326, 1617
FokI GGATG 3 cut(s) 59, 205, 627
Fsp4HI GCNGC 8 cut(s) 186, 294, 420, 660, 663, 1188, 1326, 1617
FspBI CTAG 5 cut(s) 206, 932, 1109, 1386, 1526
GluI GCNGC 8 cut(s) 186, 294, 420, 660, 663, 1188, 1326, 1617
GsuI CTGGAG 1 cut(s) 1120
HaeIII GGCC 3 cut(s) 309, 384, 415
HapII CCGG 4 cut(s) 76, 416, 1646, 1703
HgaI GACGC 1 cut(s) 745
Hin1I GRCGYC 1 cut(s) 756
Hin1II CATG 5 cut(s) 113, 229, 349, 940, 1270
HindIII AAGCTT 1 cut(s) 877
HinfI GANTC 5 cut(s) 788, 1006, 1204, 1498, 1699
HpaII CCGG 4 cut(s) 76, 416, 1646, 1703
HphI GGTGA 3 cut(s) 286, 849, 1153
Hpy166II GTNNAC 5 cut(s) 106, 958, 1010, 1244, 1433
Hpy188I TCNGA 3 cut(s) 38, 364, 782
Hpy188III TCNNGA 8 cut(s) 88, 437, 771, 906, 1027, 1099, 1505, 1703
Hpy8I GTNNAC 5 cut(s) 106, 958, 1010, 1244, 1433
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 958, 997
HpyCH4III ACNGT 3 cut(s) 145, 390, 962
HpyCH4IV ACGT 5 cut(s) 134, 607, 913, 1129, 1136
HpyCH4V TGCA 4 cut(s) 72, 359, 665, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 671
HpyF3I CTNAG 2 cut(s) 943, 1684
HpySE526I ACGT 5 cut(s) 134, 607, 913, 1129, 1136
Hsp92I GRCGYC 1 cut(s) 756
Hsp92II CATG 5 cut(s) 113, 229, 349, 940, 1270
Kpn2I TCCGGA 1 cut(s) 1702
KroI GCCGGC 1 cut(s) 415
KroNI GCCGGC 1 cut(s) 417
Ksp22I TGATCA 1 cut(s) 463
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 4 cut(s) 377, 636, 981, 1085
Lsp1109I GCAGC 5 cut(s) 172, 280, 406, 649, 1174
LweI GCATC 3 cut(s) 81, 368, 802
MaeI CTAG 5 cut(s) 206, 932, 1109, 1386, 1526
MaeII ACGT 5 cut(s) 134, 607, 913, 1129, 1136
MaeIII GTNAC 1 cut(s) 1104
MflI RGATCY 3 cut(s) 686, 823, 1397
MhlI GDGCHC 1 cut(s) 986
MlyI GAGTC 2 cut(s) 1015, 1693
Mph1103I ATGCAT 2 cut(s) 74, 361
MroI TCCGGA 1 cut(s) 1702
MroNI GCCGGC 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 6 cut(s) 270, 333, 452, 1035, 1218, 1283
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 4 cut(s) 76, 416, 1646, 1703
MspR9I CCNGG 6 cut(s) 77, 311, 646, 828, 1421, 1647
Mva1269I GAATGC 2 cut(s) 295, 1272
MvaI CCWGG 4 cut(s) 311, 646, 828, 1421
MvnI CGCG 2 cut(s) 722, 764
MwoI GCNNNNNNNGC 2 cut(s) 191, 671
NaeI GCCGGC 1 cut(s) 417
NciI CCSGG 2 cut(s) 77, 1647
NgoMIV GCCGGC 1 cut(s) 415
NlaIII CATG 5 cut(s) 113, 229, 349, 940, 1270
NlaIV GGNNCC 2 cut(s) 638, 1399
NsiI ATGCAT 2 cut(s) 74, 361
PciSI GCTCTTC 1 cut(s) 17
PctI GAATGC 2 cut(s) 295, 1272
PdiI GCCGGC 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 3 cut(s) 788, 1204, 1498
PfoI TCCNGGA 1 cut(s) 826
PkrI GCNGC 8 cut(s) 187, 295, 421, 661, 664, 1189, 1327, 1618
PleI GAGTC 2 cut(s) 1014, 1693
PpsI GAGTC 2 cut(s) 1014, 1693
Psp1406I AACGTT 1 cut(s) 913
Psp6I CCWGG 4 cut(s) 309, 644, 826, 1419
PspGI CCWGG 4 cut(s) 309, 644, 826, 1419
PspN4I GGNNCC 2 cut(s) 638, 1399
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 3 cut(s) 686, 823, 1397
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 3 cut(s) 137, 1432, 1514
RsaNI GTAC 3 cut(s) 136, 1431, 1513
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 6 cut(s) 270, 333, 452, 1035, 1218, 1283
SatI GCNGC 8 cut(s) 186, 294, 420, 660, 663, 1188, 1326, 1617
Sau96I GGNCC 1 cut(s) 263
ScaI AGTACT 1 cut(s) 1514
SchI GAGTC 2 cut(s) 1015, 1693
ScrFI CCNGG 6 cut(s) 77, 311, 646, 828, 1421, 1647
SduI GDGCHC 1 cut(s) 986
SfaNI GCATC 3 cut(s) 81, 368, 802
SfcI CTRYAG 2 cut(s) 189, 1516
SinI GGWCC 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SpeI ACTAGT 2 cut(s) 1108, 1525
SsiI CCGC 9 cut(s) 341, 627, 659, 722, 1325, 1352, 1443, 1617, 1694
SspI AATATT 2 cut(s) 449, 1664
SspMI CTAG 5 cut(s) 206, 932, 1109, 1386, 1526
StyD4I CCNGG 6 cut(s) 75, 309, 644, 826, 1419, 1645
StyI CCWWGG 2 cut(s) 57, 1587
TaaI ACNGT 3 cut(s) 145, 390, 962
TaiI ACGT 5 cut(s) 137, 610, 916, 1132, 1139
TaqI TCGA 6 cut(s) 438, 518, 551, 1341, 1357, 1724
TaqII GACCGA 1 cut(s) 251
TatI WGTACW 1 cut(s) 1512
TauI GCSGC 3 cut(s) 662, 1328, 1619
TfiI GAWTC 3 cut(s) 788, 1204, 1498
Tru1I TTAA 6 cut(s) 270, 333, 452, 1035, 1218, 1283
Tru9I TTAA 6 cut(s) 270, 333, 452, 1035, 1218, 1283
TscAI CASTG 1 cut(s) 1414
TseI GCWGC 5 cut(s) 185, 293, 419, 662, 1187
TspDTI ATGAA 9 cut(s) 235, 318, 362, 640, 801, 940, 1522, 1644, 1733
TspGWI ACGGA 4 cut(s) 390, 1072, 1110, 1244
TspRI CASTG 1 cut(s) 1414
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 3 cut(s) 150, 431, 1260
XmiI GTMKAC 2 cut(s) 957, 1009
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 5 cut(s) 206, 932, 1109, 1386, 1526
ZrmI AGTACT 1 cut(s) 1514
Zsp2I ATGCAT 2 cut(s) 74, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.