Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0001774

Physical Location & Seq

Genomic Coordinates Forward (+)

43782056 .. 43787802

Gene Structure 5747 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0265700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3384 bp

ATGGCATTCAAAATGAACTTGCTCATATTCATCTCACTCTTGGCTTTCTCAGTTGGCTCCAAGCTTTCATATTCAGACAACGCAGCGGTGCCGGAGTTCACATTCAGTTGGCTGGACGATAAAGACACATATCAAGCCGGCGAAATCGCCACCATAAGCATCAGAGCACTAAACAACTTTGACAAGCTTGACAAGAATGCGTTCAAGCCCACTCTTACAGTAGATGGAAAAATTGGGAATAGCTCTGTAGTATCTGGGGTTCTTTCAGATTTTGAAGGAGACCCTACTAGTTGGAAGCTTTTCTTTACTACAATAACAGCCGGGTTGTTCAACGTGTTGATCAATGAGGACCACTACAAAGTGTTTGATTCATCTCTGCATTTCCAAGTTCATCCAGGTAAGTTATACCCATCTGTCTGTGTTGCTTCATGGATGGGTTTCGTCCACGAGTTTGAAGCTGGTGCTCAAGCTAGAGTACAGATACTTCCGAAGGATGCATTTGGGAATAAGATCAATGAAACCAGTGAAGATCCAAGGGTACATAATTTTACAGTGTCTGTATTTCATGCAAATGATTCCATTGCAAGCATACCGAACATCACCCACTTTGGTTGGAACCAATTTGGTTATATCATCATGGAATTCATTGTGGTAAAAGCTGGAGACCTCTTGTTGCATGTGGAAGGTGGTAATCAGAGCTTAAATGGCTGTCCACTACCCTTTAAGGTGAACCCAGGACCTCTTGAAGTTTCCAATTGTGTGGTGAAATGGGATTTTGAACCAAATGCATGGCAATTATATTCCAAGATGGAAATCTTTATATACCAGCAAGATCAATATGGTAACCTTGTTCCAGGACTATATGAATTTGATGCTGAAGTTGTTGAGACAGAGACGAATTTGTCAATACCTGTTCCAGATTTGCACTTTGAAGAAGTGGCGGCTGGAATTCAGCTGTTTTCTTTTACTAATCTGGAACCAGGCAACTTTTTGCTGACTATATCAGATATGAAGCATGATAAAAGCATCTCTAATATGCCATATGCTTATACCGTCTTTGTTGGCTATTGCAATGGGACAAAAAGTGTTGTTAATGGAACTGGTTTAAATAGCTCCGTGGCAGGAGAAAGATCAGAATTCTCAGTTTATTTGAATGATGCTTATCAAAATCCCTCTCCAGTTGAAATCGAAAGGCTTGAAATACAAATTGTAAGGGAAATTGACTCCTATCATGTGCAGTCGAGCATATTTCCTATCCAGATCATCAATGGCAGTGGACCTGCTCAAGGAATAAGATATGGTACAACCAGCCAAATAGAATTTGCTCCTTCACCATCTATGACCTTGACAAATTCTACTGTCGGGAGCTTCAGCACTCTGGCAACTGCTCTCAATGTGGTATATACACCTGACAAGAGTGGGATTTATAAAATTTATGTGTTTTGTGGAAATATTTTGCTGAATGGAGGCCGTCCCTTTAATAAGGAAGTTATAGCAGGGAGTGTTGATCCATCATGCTCAACCGTTGTCAAATTTTCTCCCAAGGTGGCAAGGACGATTAATAATGAAGTAGTAGTGAAGCTCGAGGATTCATTTTTCAATCCCGTCTTGTCACAACAATCTGGGCTGAAACTAGAGATTGTTTCTGTAAACAGTTCTGGCTTTTCAAGTTCCTTGTTTGTGGACAATAATGATGGGTCATACACTGTACACTATCTAGCTAAGGAGGTTGGAACTTATGAGATGTGTGCTTCATTTGATGGCAAACGTATTTCTGCCTGCCCCTTTGGGGTCAATGTCTACAGTAGCGAATATTTTCCCAGAGCCGAAAATGATACAATTTCTGTGTGGGAGGATGAGTCAACTGCTTTTGATGTTTTGGCCAATGACTACTTTGCTGGAAATAATGCTAGTGTTGTTGAATTCTCCAAGCCAGGTCATGGTTCCCTTATTGAGTATGGAAGGCTCCTCCGGTATACACCTTATAAAGACTATTATGGAAATGATTCCTTTGTGTACACAATGTCTGATATAAACGGCAATCTTGCTACTGCTTTTGTAAACATATCTGTTCTCACCATCCCACCACAATTTGTTTCTTTTCCAAGTGAATTGCAAGCTACTGAAGACAAGATTAATCCAAAATTTGGTGGTTTCTCTGGATTTATGATAAGATATTCCGACATGATGGAGAACATCTCTGTTAATCTTAGTGCGCAATCTGGGACAGTATTTCTGTCTCCGATGCTAATGCAATTCTGGGAGCCCATTTGGAATGGACTTTCTGTGTACAAAGAGGATGGAGGAGTGAAGGGGTTGATATTACAAGGCAGTGTGGAAGTAATCAATTTTGCCCTTAAGTCAATACAGTACTATGGTAACGAGAACTTCTGTGGCTATGATACTATTCGGCTCACTACGAGGAATAGGAATGGAGTAAATGACTTGCATATTCCAGTTTTTGTGGAACCTATCAATGATCCTCCCTTTATTCATGCACCTGCGTTTATCATTCTGAAAAGTAATGAAGATGAGTCACTGATATATGACAGAGAAACAGACAAGTTTCAGTTCTATATTGGAGACCCAGATCTTCTTGCGTTCCCTGGTAATGAATCCCTTTTTTCGGTCATCTTCTCTATGGAAGTTAATGATGGATATTTGGTAACCAGCCTACCAGCTGAGCTCATCACTACAATAGAAGTGAAGACTAAGAATAGCTACCAGTGGCTACCACTTCAGACATATGTTACCATCTCAAAGCATTTCAAGGTCAAAGCTAAGGGAATCAGATTTTATGCAACAGTTAATGAATGCAATACTGCCATGCAACAGCTGTTTTATCATGGTGGAGGAGAGCAAGATGCCATTTTGACAGTGACATTGAATGACCTGGGAAACTATGGGTGTTATTGTAACTGTTCCGAAAAGATATCAGTGCCCTTACTTGCTGAGGCTTCTGTTAATCTAATAAGAAGAAGGCCGATGAGTTCACTTGTGTCTCGTGGCTTAGGATCAGCTATTGTCATTGAGTCCATTATAGTGTTTTCACTGGGAGCAGCACTAATGTTCTTCACATGCAAATGTGCAACTATCCTTGTAAATGAAAGAAGAAACAGAGCTACCAAGACTCCAGCTGACCCAGCAAGTGCACATAGTTTGGGCAAAGGAAATTCGGGTAAAAGTTTACCAGAGAACGCAACTCACCTTACCGCGTCTTGTTCAAGTCCCCTGCTTGGCAGCCAACGTCCCAACTTTAGACAACGGTCCTGCCGTCAATCTGGAAATGGGGAATCAAGCAAGGCAGCATATAAGGCTACCGGTGAACGCATTCAGCAGAATCCTGCGCCTAGTTTTAAGCCCTTTTGTGTTGAGAAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF707

Protein Analysis

1127

Amino Acids

124.48

Weight (kDa)

5.1

Isoelectric Point (pI)

35.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_GEX2_N	PF23616	31 - 130	3.9e-30	GEX2 N-terminal Ig-like domain
Ig_GEX2_N	PF23616	138 - 243	3.3e-36	GEX2 N-terminal Ig-like domain
Filamin	PF00630	543 - 596	3.1e-06	Filamin/ABP280 repeat
Big_9	PF17963	607 - 689	3.4e-13	Bacterial Ig domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67850 AT1G67850 AT1G67850 AT1G67850 AT1G67850 AT1G67850 AT1G67850
fragaria_vesca	FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710 FvH4_4g25710
malus_domestica	MD13G1099800.v1.1 MD16G1100800.v1.1
prunus_persica	Prupe.1G246200_v2.0.a1 Prupe.1G246200_v2.0.a1 Prupe.1G246200_v2.0.a1
pyrus_communis	pycom13g08720 pycom16g08530
rosa_chinensis	RchiOBHm_Chr4g0433141
rosa_laevigata	RLG00000006781
rosa_multiflora	Rmu_ssc0000295.1_g000051
rosa_roxburghii	Rroxscaffold_5G00374620
rosa_rugosa	Rorug04G0265700 Rorug04G0265800 Rorug04G0265800 Rorug04G0265900 Rorug04G0266000 Rorug04G0266100 Rorug04G0266200.1
rosa_samantha	Rh4AG319800 Rh4BG328100 Rh4CG343500 Rh4DG323300
rosa_wichuraiana	Rw4G027780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1428, 1986
AarI	CACCTGC	1 cut(s)	2509
AasI	GACNNNNNNGTC	1 cut(s)	901
Acc16I	TGCGCA	1 cut(s)	2217
Acc36I	ACCTGC	2 cut(s)	1288, 2509
AccB1I	GGYRCC	1 cut(s)	88
AccB7I	CCANNNNNTGG	2 cut(s)	1940, 2147
AccI	GTMKAC	2 cut(s)	1800, 1976
AccII	CGCG	1 cut(s)	3215
AciI	CCGC	3 cut(s)	86, 941, 3213
AclWI	GGATC	4 cut(s)	524, 1502, 2474, 3022
AcoI	YGGCCR	1 cut(s)	1881
AcuI	CTGAAG	4 cut(s)	897, 1354, 2145, 2723
AfaI	GTAC	7 cut(s)	477, 540, 1303, 1710, 2018, 2291, 2372
AfiI	CCNNNNNNNGG	6 cut(s)	1286, 1789, 1940, 2147, 2626, 3236
AflII	CTTAAG	1 cut(s)	2357
AflIII	ACRYGT	1 cut(s)	333
AgeI	ACCGGT	1 cut(s)	3320
AhlI	ACTAGT	1 cut(s)	287
AjnI	CCWGG	7 cut(s)	394, 733, 853, 979, 1933, 2605, 2892
Alw21I	GWGCWC	4 cut(s)	169, 466, 2688, 3154
Alw26I	GTCTC	7 cut(s)	273, 657, 881, 887, 2244, 2577, 3006
Alw44I	GTGCAC	1 cut(s)	3150
AlwI	GGATC	4 cut(s)	524, 1502, 2474, 3022
AlwNI	CAGNNNCTG	1 cut(s)	557
Ama87I	CYCGRG	1 cut(s)	1583
AoxI	GGCC	3 cut(s)	1468, 1881, 2981
ApaLI	GTGCAC	1 cut(s)	3150
ApeKI	GCWGC	4 cut(s)	83, 3059, 3240, 3305
AseI	ATTAAT	2 cut(s)	1560, 2136
AsiGI	ACCGGT	1 cut(s)	3320
Asp700I	GAANNNNTTC	5 cut(s)	200, 299, 1815, 2005, 3330
AspLEI	GCGC	2 cut(s)	2218, 3349
AspS9I	GGNCC	4 cut(s)	349, 737, 1277, 3267
AsuC2I	CCSGG	1 cut(s)	322
AsuHPI	GGTGA	7 cut(s)	592, 739, 775, 1323, 2068, 3197, 3335
AvaI	CYCGRG	1 cut(s)	1583
AvaII	GGWCC	4 cut(s)	349, 737, 1277, 3267
BaeGI	GKGCMC	2 cut(s)	2943, 3154
BalI	TGGCCA	1 cut(s)	1883
BanI	GGYRCC	1 cut(s)	88
BanII	GRGCYC	2 cut(s)	2268, 2688
BarI	GAAGNNNNNNTAC	2 cut(s)	468, 500
BauI	CACGAG	2 cut(s)	446, 3003
BbsI	GAAGAC	2 cut(s)	2133, 2714
Bbv12I	GWGCWC	4 cut(s)	169, 466, 2688, 3154
BbvCI	CCTCAGC	1 cut(s)	2952
BbvI	GCAGC	4 cut(s)	95, 3071, 3252, 3317
BceAI	ACGGC	3 cut(s)	1455, 2053, 3258
BcgI	CGANNNNNNTGC	2 cut(s)	2233, 2267
BciT130I	CCWGG	7 cut(s)	396, 735, 855, 981, 1935, 2607, 2894
BclI	TGATCA	1 cut(s)	339
BcnI	CCSGG	1 cut(s)	322
BcoDI	GTCTC	7 cut(s)	273, 657, 881, 887, 2244, 2577, 3006
BcuI	ACTAGT	1 cut(s)	287
BfaI	CTAG	6 cut(s)	288, 471, 1634, 1718, 1911, 3351
BfmI	CTRYAG	2 cut(s)	246, 1801
BfrI	CTTAAG	1 cut(s)	2357
BfuAI	ACCTGC	2 cut(s)	1288, 2509
BglII	AGATCT	1 cut(s)	2590
BisI	GCNGC	5 cut(s)	84, 942, 3060, 3241, 3306
BlpI	GCTNAGC	1 cut(s)	2682
BlsI	GCNGC	5 cut(s)	85, 943, 3061, 3242, 3307
BmcAI	AGTACT	1 cut(s)	2372
Bme1390I	CCNGG	8 cut(s)	322, 396, 735, 855, 981, 1935, 2607, 2894
Bme18I	GGWCC	4 cut(s)	349, 737, 1277, 3267
BmeT110I	CYCGRG	1 cut(s)	1583
BmgT120I	GGNCC	4 cut(s)	349, 737, 1277, 3267
BmiI	GGNNCC	8 cut(s)	58, 90, 617, 978, 1945, 1967, 2265, 2469
BmrFI	CCNGG	8 cut(s)	322, 396, 735, 855, 981, 1935, 2607, 2894
BmrI	ACTGGG	1 cut(s)	3062
BmsI	GCATC	7 cut(s)	168, 484, 862, 1035, 1147, 2235, 2854
BmuI	ACTGGG	1 cut(s)	3062
BoxI	GACNNNNGTC	1 cut(s)	3265
BpiI	GAAGAC	2 cut(s)	2133, 2714
BplI	GAGNNNNNCTC	2 cut(s)	2183, 2215
BpmI	CTGGAG	3 cut(s)	681, 1161, 3117
Bpu10I	CCTNAGC	4 cut(s)	1722, 2781, 2952, 3010
Bpu1102I	GCTNAGC	1 cut(s)	2682
BpuEI	CTTGAG	2 cut(s)	450, 1269
BpuMI	CCSGG	1 cut(s)	322
BsaBI	GATNNNNATC	2 cut(s)	812, 1161
BsaI	GGTCTC	3 cut(s)	273, 657, 2577
BsaJI	CCNNGG	6 cut(s)	533, 733, 1116, 1542, 2605, 2893
BsaWI	WCCGGW	2 cut(s)	1971, 3320
BsaXI	ACNNNNNCTCC	4 cut(s)	1844, 1874, 3115, 3145
Bsc4I	CCNNNNNNNGG	6 cut(s)	1286, 1789, 1940, 2147, 2626, 3236
Bse118I	RCCGGY	2 cut(s)	137, 3320
Bse1I	ACTGG	6 cut(s)	522, 1105, 1178, 2456, 2725, 3057
Bse3DI	GCAATG	2 cut(s)	579, 1078
Bse8I	GATNNNNATC	2 cut(s)	812, 1161
BseBI	CCWGG	7 cut(s)	396, 735, 855, 981, 1935, 2607, 2894
BseDI	CCNNGG	6 cut(s)	533, 733, 1116, 1542, 2605, 2893
BseGI	GGATG	6 cut(s)	391, 438, 499, 1861, 2079, 2305
BseJI	GATNNNNATC	2 cut(s)	812, 1161
BseLI	CCNNNNNNNGG	6 cut(s)	1286, 1789, 1940, 2147, 2626, 3236
BseMI	GCAATG	2 cut(s)	579, 1078
BseMII	CTCAG	4 cut(s)	63, 1155, 2673, 2943
BseNI	ACTGG	6 cut(s)	522, 1105, 1178, 2456, 2725, 3057
BseRI	GAGGAG	3 cut(s)	1958, 2319, 2868
BseSI	GKGCMC	2 cut(s)	2943, 3154
BseXI	GCAGC	4 cut(s)	95, 3071, 3252, 3317
BseYI	CCCAGC	1 cut(s)	3142
BsgI	GTGCAG	1 cut(s)	1256
Bsh1236I	CGCG	1 cut(s)	3215
BshFI	GGCC	3 cut(s)	1470, 1883, 2983
BshNI	GGYRCC	1 cut(s)	88
BshTI	ACCGGT	1 cut(s)	3320
BsiHKAI	GWGCWC	4 cut(s)	169, 466, 2688, 3154
BsiHKCI	CYCGRG	1 cut(s)	1583
BsiSI	CCGG	5 cut(s)	92, 138, 321, 1972, 3321
BslFI	GGGAC	5 cut(s)	1090, 1458, 2239, 3213, 3234
BslI	CCNNNNNNNGG	6 cut(s)	1286, 1789, 1940, 2147, 2626, 3236
BsmAI	GTCTC	7 cut(s)	273, 657, 881, 887, 2244, 2577, 3006
BsmBI	CGTCTC	1 cut(s)	887
BsmFI	GGGAC	5 cut(s)	1090, 1458, 2239, 3213, 3234
BsmI	GAATGC	4 cut(s)	5, 202, 2819, 3330
BsnI	GGCC	3 cut(s)	1470, 1883, 2983
Bso31I	GGTCTC	3 cut(s)	273, 657, 2577
BsoBI	CYCGRG	1 cut(s)	1583
Bsp1286I	GDGCHC	6 cut(s)	169, 466, 2268, 2688, 2943, 3154
Bsp1407I	TGTACA	3 cut(s)	1708, 2016, 2289
Bsp1720I	GCTNAGC	1 cut(s)	2682
BspACI	CCGC	3 cut(s)	86, 941, 3213
BspANI	GGCC	3 cut(s)	1470, 1883, 2983
BspCNI	CTCAG	4 cut(s)	62, 1154, 2674, 2944
BspFNI	CGCG	1 cut(s)	3215
BspLI	GGNNCC	8 cut(s)	58, 90, 617, 978, 1945, 1967, 2265, 2469
BspMI	ACCTGC	2 cut(s)	1288, 2509
BspPI	GGATC	4 cut(s)	524, 1502, 2474, 3022
BspT107I	GGYRCC	1 cut(s)	88
BspTI	CTTAAG	1 cut(s)	2357
BspTNI	GGTCTC	3 cut(s)	273, 657, 2577
BsrDI	GCAATG	2 cut(s)	579, 1078
BsrFI	RCCGGY	2 cut(s)	137, 3320
BsrGI	TGTACA	3 cut(s)	1708, 2016, 2289
BsrI	ACTGG	6 cut(s)	522, 1105, 1178, 2456, 2725, 3057
BssAI	RCCGGY	2 cut(s)	137, 3320
BssECI	CCNNGG	6 cut(s)	533, 733, 1116, 1542, 2605, 2893
BssNAI	GTATAC	1 cut(s)	1977
BssSI	CACGAG	2 cut(s)	446, 3003
BssT1I	CCWWGG	2 cut(s)	533, 1542
Bst1107I	GTATAC	1 cut(s)	1977
Bst2BI	CACGAG	2 cut(s)	446, 3003
Bst2UI	CCWGG	7 cut(s)	396, 735, 855, 981, 1935, 2607, 2894
BstAFI	CTTAAG	1 cut(s)	2357
BstAUI	TGTACA	3 cut(s)	1708, 2016, 2289
BstC8I	GCNNGC	4 cut(s)	139, 586, 1780, 2118
BstDEI	CTNAG	9 cut(s)	49, 1141, 1722, 2210, 2682, 2712, 2781, 2952, 3010
BstDSI	CCRYGG	1 cut(s)	1116
BstEII	GGTNACC	2 cut(s)	842, 2665
BstENI	CCTNNNNNAGG	1 cut(s)	1284
BstF5I	GGATG	6 cut(s)	391, 438, 499, 1861, 2079, 2305
BstFNI	CGCG	1 cut(s)	3215
BstHHI	GCGC	2 cut(s)	2218, 3349
BstMAI	GTCTC	7 cut(s)	273, 657, 881, 887, 2244, 2577, 3006
BstMWI	GCNNNNNNNGC	3 cut(s)	705, 3143, 3314
BstNI	CCWGG	7 cut(s)	396, 735, 855, 981, 1935, 2607, 2894
BstNSI	RCATGY	2 cut(s)	680, 3081
BstPAI	GACNNNNGTC	1 cut(s)	3265
BstPI	GGTNACC	2 cut(s)	842, 2665
BstSCI	CCNGG	8 cut(s)	320, 394, 733, 853, 979, 1933, 2605, 2892
BstSFI	CTRYAG	2 cut(s)	246, 1801
BstSLI	GKGCMC	2 cut(s)	2943, 3154
BstUI	CGCG	1 cut(s)	3215
BstV1I	GCAGC	4 cut(s)	95, 3071, 3252, 3317
BstV2I	GAAGAC	2 cut(s)	2133, 2714
BstX2I	RGATCY	2 cut(s)	529, 2590
BstXI	CCANNNNNNTGG	2 cut(s)	760, 789
BstYI	RGATCY	2 cut(s)	529, 2590
BstZ17I	GTATAC	1 cut(s)	1977
BsuRI	GGCC	3 cut(s)	1470, 1883, 2983
BtgI	CCRYGG	1 cut(s)	1116
BtsCI	GGATG	6 cut(s)	391, 438, 499, 1861, 2079, 2305
BtsI	GCAGTG	2 cut(s)	1279, 2338
BveI	ACCTGC	2 cut(s)	1288, 2509
Cac8I	GCNNGC	4 cut(s)	139, 586, 1780, 2118
CaiI	CAGNNNCTG	1 cut(s)	557
CfoI	GCGC	2 cut(s)	2218, 3349
Cfr10I	RCCGGY	2 cut(s)	137, 3320
Cfr13I	GGNCC	4 cut(s)	349, 737, 1277, 3267
CseI	GACGC	1 cut(s)	3204
Csp6I	GTAC	7 cut(s)	476, 539, 1302, 1709, 2017, 2290, 2371
CspAI	ACCGGT	1 cut(s)	3320
CspCI	CAANNNNNGTGG	4 cut(s)	593, 628, 1255, 1290
CviQI	GTAC	7 cut(s)	476, 539, 1302, 1709, 2017, 2290, 2371
DdeI	CTNAG	9 cut(s)	49, 1141, 1722, 2210, 2682, 2712, 2781, 2952, 3010
DraI	TTTAAA	1 cut(s)	1107
DrdI	GACNNNNNNGTC	1 cut(s)	901
DseDI	GACNNNNNNGTC	1 cut(s)	901
EaeI	YGGCCR	1 cut(s)	1881
Ecl136II	GAGCTC	1 cut(s)	2686
Eco130I	CCWWGG	2 cut(s)	533, 1542
Eco24I	GRGCYC	2 cut(s)	2268, 2688
Eco31I	GGTCTC	3 cut(s)	273, 657, 2577
Eco32I	GATATC	1 cut(s)	2934
Eco47I	GGWCC	4 cut(s)	349, 737, 1277, 3267
Eco53kI	GAGCTC	1 cut(s)	2686
Eco57I	CTGAAG	4 cut(s)	897, 1354, 2145, 2723
Eco88I	CYCGRG	1 cut(s)	1583
Eco91I	GGTNACC	2 cut(s)	842, 2665
EcoICRI	GAGCTC	1 cut(s)	2686
EcoNI	CCTNNNNNAGG	1 cut(s)	1284
EcoO109I	RGGNCCY	1 cut(s)	737
EcoO65I	GGTNACC	2 cut(s)	842, 2665
EcoRI	GAATTC	4 cut(s)	641, 948, 1136, 1922
EcoRII	CCWGG	7 cut(s)	394, 733, 853, 979, 1933, 2605, 2892
EcoRV	GATATC	1 cut(s)	2934
EcoT14I	CCWWGG	2 cut(s)	533, 1542
EcoT22I	ATGCAT	2 cut(s)	499, 790
EcoT38I	GRGCYC	2 cut(s)	2268, 2688
ErhI	CCWWGG	2 cut(s)	533, 1542
Esp3I	CGTCTC	1 cut(s)	887
FalI	AAGNNNNNCTT	2 cut(s)	287, 319
FaqI	GGGAC	5 cut(s)	1090, 1458, 2239, 3213, 3234
FauNDI	CATATG	2 cut(s)	1042, 2746
FbaI	TGATCA	1 cut(s)	339
FblI	GTMKAC	2 cut(s)	1800, 1976
Fnu4HI	GCNGC	5 cut(s)	84, 942, 3060, 3241, 3306
FokI	GGATG	6 cut(s)	378, 445, 506, 1868, 2066, 2312
FriOI	GRGCYC	2 cut(s)	2268, 2688
Fsp4HI	GCNGC	5 cut(s)	84, 942, 3060, 3241, 3306
FspBI	CTAG	6 cut(s)	288, 471, 1634, 1718, 1911, 3351
FspI	TGCGCA	1 cut(s)	2217
GlaI	GCGC	2 cut(s)	2217, 3348
GluI	GCNGC	5 cut(s)	84, 942, 3060, 3241, 3306
GsaI	CCCAGC	1 cut(s)	3146
GsuI	CTGGAG	3 cut(s)	681, 1161, 3117
HaeIII	GGCC	3 cut(s)	1470, 1883, 2983
HapII	CCGG	5 cut(s)	92, 138, 321, 1972, 3321
HgaI	GACGC	1 cut(s)	3204
HhaI	GCGC	2 cut(s)	2218, 3349
Hin6I	GCGC	2 cut(s)	2216, 3347
HinP1I	GCGC	2 cut(s)	2216, 3347
HincII	GTYRAC	1 cut(s)	1863
HindII	GTYRAC	1 cut(s)	1863
HindIII	AAGCTT	3 cut(s)	62, 185, 296
HpaII	CCGG	5 cut(s)	92, 138, 321, 1972, 3321
HphI	GGTGA	7 cut(s)	592, 739, 775, 1323, 2068, 3197, 3335
Hpy188III	TCNNGA	7 cut(s)	743, 917, 974, 1258, 1363, 2160, 3282
HpyAV	CCTTC	8 cut(s)	269, 484, 677, 1338, 1956, 2305, 2973, 3370
HpyCH4IV	ACGT	3 cut(s)	333, 1768, 3247
HpyF10VI	GCNNNNNNNGC	3 cut(s)	705, 3143, 3314
HpyF3I	CTNAG	9 cut(s)	49, 1141, 1722, 2210, 2682, 2712, 2781, 2952, 3010
HpySE526I	ACGT	3 cut(s)	333, 1768, 3247
HspAI	GCGC	2 cut(s)	2216, 3347
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
Ksp22I	TGATCA	1 cut(s)	339
LmnI	GCTCC	7 cut(s)	62, 1118, 1330, 1365, 1971, 2263, 3056
Lsp1109I	GCAGC	4 cut(s)	95, 3071, 3252, 3317
LweI	GCATC	7 cut(s)	168, 484, 862, 1035, 1147, 2235, 2854
MaeI	CTAG	6 cut(s)	288, 471, 1634, 1718, 1911, 3351
MaeII	ACGT	3 cut(s)	333, 1768, 3247
MaeIII	GTNAC	8 cut(s)	842, 1611, 2378, 2535, 2665, 2749, 2878, 2915
MfeI	CAATTG	1 cut(s)	754
MflI	RGATCY	2 cut(s)	529, 2590
MhlI	GDGCHC	6 cut(s)	169, 466, 2268, 2688, 2943, 3154
MlsI	TGGCCA	1 cut(s)	1883
MluNI	TGGCCA	1 cut(s)	1883
MlyI	GAGTC	5 cut(s)	1217, 1868, 2543, 3041, 3124
MmeI	TCCRAC	4 cut(s)	272, 593, 1711, 2205
Mox20I	TGGCCA	1 cut(s)	1883
Mph1103I	ATGCAT	2 cut(s)	499, 790
MroNI	GCCGGC	1 cut(s)	137
MroXI	GAANNNNTTC	5 cut(s)	200, 299, 1815, 2005, 3330
MscI	TGGCCA	1 cut(s)	1883
MslI	CAYNNNNRTG	3 cut(s)	570, 3041, 3082
Msp20I	TGGCCA	1 cut(s)	1883
MspA1I	CMGCKG	5 cut(s)	86, 955, 2681, 2836, 3137
MspCI	CTTAAG	1 cut(s)	2357
MspI	CCGG	5 cut(s)	92, 138, 321, 1972, 3321
MspR9I	CCNGG	8 cut(s)	322, 396, 735, 855, 981, 1935, 2607, 2894
MunI	CAATTG	1 cut(s)	754
Mva1269I	GAATGC	4 cut(s)	5, 202, 2819, 3330
MvaI	CCWGG	7 cut(s)	396, 735, 855, 981, 1935, 2607, 2894
MvnI	CGCG	1 cut(s)	3215
MwoI	GCNNNNNNNGC	3 cut(s)	705, 3143, 3314
NaeI	GCCGGC	1 cut(s)	139
NciI	CCSGG	1 cut(s)	322
NdeI	CATATG	2 cut(s)	1042, 2746
NgoMIV	GCCGGC	1 cut(s)	137
NlaIV	GGNNCC	8 cut(s)	58, 90, 617, 978, 1945, 1967, 2265, 2469
NmuCI	GTSAC	3 cut(s)	1611, 2535, 2878
NsbI	TGCGCA	1 cut(s)	2217
NsiI	ATGCAT	2 cut(s)	499, 790
NspI	RCATGY	2 cut(s)	680, 3081
PaeR7I	CTCGAG	1 cut(s)	1583
PaqCI	CACCTGC	1 cut(s)	2509
PcsI	WCGNNNNNNNCGW	1 cut(s)	3271
PctI	GAATGC	4 cut(s)	5, 202, 2819, 3330
PdiI	GCCGGC	1 cut(s)	139
PdmI	GAANNNNTTC	5 cut(s)	200, 299, 1815, 2005, 3330
PfeI	GAWTC	8 cut(s)	368, 575, 1589, 2006, 2615, 2787, 3293, 3340
PflMI	CCANNNNNTGG	2 cut(s)	1940, 2147
PfoI	TCCNGGA	1 cut(s)	853
PinAI	ACCGGT	1 cut(s)	3320
PkrI	GCNGC	5 cut(s)	85, 943, 3061, 3242, 3307
PleI	GAGTC	5 cut(s)	1217, 1867, 2542, 3040, 3124
PpsI	GAGTC	5 cut(s)	1217, 1867, 2542, 3040, 3124
PpuMI	RGGWCCY	1 cut(s)	737
PshAI	GACNNNNGTC	1 cut(s)	3265
PshBI	ATTAAT	2 cut(s)	1560, 2136
PsiI	TTATAA	2 cut(s)	1428, 1986
Psp124BI	GAGCTC	1 cut(s)	2688
Psp5II	RGGWCCY	1 cut(s)	737
Psp6I	CCWGG	7 cut(s)	394, 733, 853, 979, 1933, 2605, 2892
PspEI	GGTNACC	2 cut(s)	842, 2665
PspFI	CCCAGC	1 cut(s)	3142
PspGI	CCWGG	7 cut(s)	394, 733, 853, 979, 1933, 2605, 2892
PspN4I	GGNNCC	8 cut(s)	58, 90, 617, 978, 1945, 1967, 2265, 2469
PspPI	GGNCC	4 cut(s)	349, 737, 1277, 3267
PspPPI	RGGWCCY	1 cut(s)	737
PspXI	VCTCGAGB	1 cut(s)	1583
PsrI	GAACNNNNNNTAC	2 cut(s)	243, 275
PstNI	CAGNNNCTG	1 cut(s)	557
PsuI	RGATCY	2 cut(s)	529, 2590
PvuII	CAGCTG	4 cut(s)	955, 2681, 2836, 3137
RsaI	GTAC	7 cut(s)	477, 540, 1303, 1710, 2018, 2291, 2372
RsaNI	GTAC	7 cut(s)	476, 539, 1302, 1709, 2017, 2290, 2371
RseI	CAYNNNNRTG	3 cut(s)	570, 3041, 3082
SacI	GAGCTC	1 cut(s)	2688
SatI	GCNGC	5 cut(s)	84, 942, 3060, 3241, 3306
Sau96I	GGNCC	4 cut(s)	349, 737, 1277, 3267
ScaI	AGTACT	1 cut(s)	2372
SchI	GAGTC	5 cut(s)	1217, 1868, 2543, 3041, 3124
ScrFI	CCNGG	8 cut(s)	322, 396, 735, 855, 981, 1935, 2607, 2894
SduI	GDGCHC	6 cut(s)	169, 466, 2268, 2688, 2943, 3154
SfaNI	GCATC	7 cut(s)	168, 484, 862, 1035, 1147, 2235, 2854
SfcI	CTRYAG	2 cut(s)	246, 1801
Sfr274I	CTCGAG	1 cut(s)	1583
SinI	GGWCC	4 cut(s)	349, 737, 1277, 3267
SlaI	CTCGAG	1 cut(s)	1583
SmiMI	CAYNNNNRTG	3 cut(s)	570, 3041, 3082
SmlI	CTYRAG	4 cut(s)	465, 1284, 1583, 2357
SmoI	CTYRAG	4 cut(s)	465, 1284, 1583, 2357
SpeI	ACTAGT	1 cut(s)	287
SsiI	CCGC	3 cut(s)	86, 941, 3213
SspI	AATATT	2 cut(s)	1453, 1814
SspMI	CTAG	6 cut(s)	288, 471, 1634, 1718, 1911, 3351
SstI	GAGCTC	1 cut(s)	2688
StyD4I	CCNGG	8 cut(s)	320, 394, 733, 853, 979, 1933, 2605, 2892
StyI	CCWWGG	2 cut(s)	533, 1542
TaiI	ACGT	3 cut(s)	336, 1771, 3250
TaqI	TCGA	3 cut(s)	1188, 1241, 1584
TaqII	GACCGA	1 cut(s)	2617
TatI	WGTACW	5 cut(s)	475, 1708, 2016, 2289, 2370
TauI	GCSGC	1 cut(s)	944
TfiI	GAWTC	8 cut(s)	368, 575, 1589, 2006, 2615, 2787, 3293, 3340
TseFI	GTSAC	3 cut(s)	1611, 2535, 2878
TseI	GCWGC	4 cut(s)	83, 3059, 3240, 3305
Tsp45I	GTSAC	3 cut(s)	1611, 2535, 2878
TspGWI	ACGGA	1 cut(s)	1105
Van91I	CCANNNNNTGG	2 cut(s)	1940, 2147
Vha464I	CTTAAG	1 cut(s)	2357
VneI	GTGCAC	1 cut(s)	3150
VpaK11BI	GGWCC	4 cut(s)	349, 737, 1277, 3267
VspI	ATTAAT	2 cut(s)	1560, 2136
XagI	CCTNNNNNAGG	1 cut(s)	1284
XceI	RCATGY	2 cut(s)	680, 3081
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1265
XhoI	CTCGAG	1 cut(s)	1583
XmiI	GTMKAC	2 cut(s)	1800, 1976
XmnI	GAANNNNTTC	5 cut(s)	200, 299, 1815, 2005, 3330
XspI	CTAG	6 cut(s)	288, 471, 1634, 1718, 1911, 3351
ZrmI	AGTACT	1 cut(s)	2372
Zsp2I	ATGCAT	2 cut(s)	499, 790

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.