MD16G1100800.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001774
Physical Location & Seq
Forward (+)
6975400 .. 6980244
4845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1100800.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGTATAGTCAGAAGCTTGTAAATAAAATGGGAATCCTTTCACGCAGTGCGGGTGGTAGGAAACCAAATGAGACTATGAGGCTTATTGTGACAACTTTTGTAGGAGTAGTTTTTGGGTTCTTGATAGGTGCATCCTTTCCAACATTGTCACTAACAAAGCTGAATCTCTCATCCAGCCTTCGTCCAACTATTGATCTGTCATACAACGAGGATCTGAAATCAGACATCTCCAGCCAAGCACTTCTAAGTTCTTGGTCTTCTGCAAGGAGTAAAAATAGCACAAGTCAAGATCAGAGTTCAAATGGCACATTGAGGATTTGGGTGCCATCAAATCCCAGAGGTGCAGAAATGCTACCACCAGGAATTGTTGCAGCCGAGTCAGACTTCTACTTGCGCAGATTATGGGGCAATCCTAGTGAGGACGTGACCATCAAACCAAGGTACCTTGTGACATTCACTGTGGGTTTGAATCAGAAAAGGAATATCGATGCAGCAGTGAAAAAGTTTTCAGAGAACTTTACGATTCTCCTTTTTCATTATGATGGCCAAACAACTGAGTGGGATGACCTGGAGTGGTCTAAGCGTGCTATACATGTTAGTGTTCGTAAGCAGACTAAATGGTGGTATGCCAAGCGGTTTTTGCATCCTGACATTGTGGCCCCATACGACTACATATTTATCTGGGATGAAGACCTGGGAGTAGAGCATTTTAATTCAGAAGAATACATTAGACTAGTGAGGAAGCATGGTTTGGAGATTTCACAGCCTGGTTTGGAACCCAACAAAGGTTTAACATGGCAGATGACAAAGAGGAGAGGCGATCGTGAAGTTCACAAACAAACAGAGGAGAAACCGGGCTGGTGCACTGACCCACTTCTGCCTCCATGTGCAGCCTTTATTGAAATCATGGCTCCAGTATTTTCTCGTAGTGCCTGGCGCTGTGTGTGGCATATGATTCAGAATGACTTGGTCCACGGCTGGGGTCTTGACTTTGCCCTGAGAAAATGTGTAGAGCCTGCGCATGATAAGATCGGCGTTGTAGATTCTCAGTGGATCGTTCATCAAACTGTTCCTTCATTGGGGAGCCAGGGTGAATCAGAAAATGGAAAGGCGCCATGGCAAGGGGTAAGGGAAAGGTGCAGAAAGGAATGGACGATGTTTCAGAAGCGAGTATCAAATGCAGAAAATGCCTACTTCAAGGCTATCGGGGTAGATCCCTCAACTTCAAAGGCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

413

Amino Acids

47.0

Weight (kDa)

9.02

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 110 - 396 4.6e-142 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 395, 1020
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 3 cut(s) 322, 441, 1111
AciI CCGC 2 cut(s) 50, 634
AclWI GGATC 3 cut(s) 219, 1061, 1208
AcoI YGGCCR 1 cut(s) 544
AcyI GRCGYC 1 cut(s) 1112
AdeI CACNNNGTG 1 cut(s) 47
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 4 cut(s) 785, 979, 1079, 1121
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 5 cut(s) 300, 469, 902, 1198, 1227
AhlI ACTAGT 1 cut(s) 733
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 6 cut(s) 358, 567, 693, 766, 932, 1086
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 2 cut(s) 16, 160
AluI AGCT 2 cut(s) 16, 160
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 1 cut(s) 65
Alw44I GTGCAC 1 cut(s) 862
AlwI GGATC 3 cut(s) 219, 1061, 1208
AoxI GGCC 3 cut(s) 544, 657, 1230
ApaLI GTGCAC 1 cut(s) 862
ApeKI GCWGC 3 cut(s) 371, 491, 890
Asp700I GAANNNNTTC 1 cut(s) 37
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 4 cut(s) 396, 939, 1021, 1114
AspS9I GGNCC 3 cut(s) 658, 970, 1231
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 1 cut(s) 1103
AvaII GGWCC 1 cut(s) 970
BaeGI GKGCMC 1 cut(s) 866
BaeI ACNNNNGTAYC 2 cut(s) 425, 458
BalI TGGCCA 1 cut(s) 546
BanI GGYRCC 3 cut(s) 322, 441, 1111
BbsI GAAGAC 2 cut(s) 249, 696
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 3 cut(s) 383, 503, 902
BccI CCATC 3 cut(s) 334, 437, 536
BceAI ACGGC 1 cut(s) 991
BciT130I CCWGG 6 cut(s) 360, 569, 695, 768, 934, 1088
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 2 cut(s) 414, 734
BfoI RGCGCY 2 cut(s) 940, 1115
BisI GCNGC 3 cut(s) 372, 492, 891
BlsI GCNGC 3 cut(s) 373, 493, 892
Bme1390I CCNGG 7 cut(s) 360, 569, 695, 768, 855, 934, 1088
Bme18I GGWCC 1 cut(s) 970
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 3 cut(s) 658, 970, 1231
BmiI GGNNCC 7 cut(s) 324, 443, 660, 777, 912, 1085, 1113
BmrFI CCNGG 7 cut(s) 360, 569, 695, 768, 855, 934, 1088
BmsI GCATC 3 cut(s) 140, 478, 652
BpiI GAAGAC 2 cut(s) 249, 696
BpmI CTGGAG 3 cut(s) 214, 590, 897
BpuMI CCSGG 1 cut(s) 855
Bsa29I ATCGAT 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 1112
BsaJI CCNNGG 5 cut(s) 437, 694, 973, 1087, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 4 cut(s) 785, 979, 1079, 1121
Bse1I ACTGG 1 cut(s) 914
BseBI CCWGG 6 cut(s) 360, 569, 695, 768, 934, 1088
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 5 cut(s) 437, 694, 973, 1087, 1115
BseGI GGATG 5 cut(s) 131, 170, 568, 643, 691
BseLI CCNNNNNNNGG 4 cut(s) 785, 979, 1079, 1121
BseMII CTCAG 3 cut(s) 546, 989, 1061
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 2 cut(s) 826, 860
BseSI GKGCMC 1 cut(s) 866
BseXI GCAGC 3 cut(s) 383, 503, 902
BseYI CCCAGC 1 cut(s) 978
BsgI GTGCAG 3 cut(s) 363, 909, 1159
Bsh1285I CGRYCG 1 cut(s) 823
BshFI GGCC 3 cut(s) 546, 659, 1232
BshNI GGYRCC 3 cut(s) 322, 441, 1111
BshVI ATCGAT 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 866
BsiSI CCGG 1 cut(s) 854
BslI CCNNNNNNNGG 4 cut(s) 785, 979, 1079, 1121
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 3 cut(s) 546, 659, 1232
Bsp1286I GDGCHC 1 cut(s) 866
Bsp143I GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
Bsp19I CCATGG 1 cut(s) 1115
BspACI CCGC 2 cut(s) 50, 634
BspANI GGCC 3 cut(s) 546, 659, 1232
BspCNI CTCAG 3 cut(s) 547, 990, 1060
BspDI ATCGAT 1 cut(s) 486
BspLI GGNNCC 7 cut(s) 324, 443, 660, 777, 912, 1085, 1113
BspPI GGATC 3 cut(s) 219, 1061, 1208
BspT107I GGYRCC 3 cut(s) 322, 441, 1111
BsrI ACTGG 1 cut(s) 914
BssECI CCNNGG 5 cut(s) 437, 694, 973, 1087, 1115
BssMI GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
BssNI GRCGYC 1 cut(s) 1112
BssT1I CCWWGG 2 cut(s) 437, 1115
Bst2UI CCWGG 6 cut(s) 360, 569, 695, 768, 934, 1088
Bst4CI ACNGT 2 cut(s) 460, 1069
BstACI GRCGYC 1 cut(s) 1112
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstC8I GCNNGC 2 cut(s) 585, 1017
BstDEI CTNAG 5 cut(s) 245, 555, 579, 998, 1047
BstDSI CCRYGG 2 cut(s) 973, 1115
BstF5I GGATG 5 cut(s) 131, 170, 568, 643, 691
BstH2I RGCGCY 2 cut(s) 940, 1115
BstHHI GCGC 4 cut(s) 396, 939, 1021, 1114
BstKTI GATC 7 cut(s) 196, 214, 292, 823, 1032, 1056, 1216
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
BstMCI CGRYCG 1 cut(s) 823
BstMWI GCNNNNNNNGC 2 cut(s) 640, 1187
BstNI CCWGG 6 cut(s) 360, 569, 695, 768, 934, 1088
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 7 cut(s) 358, 567, 693, 766, 853, 932, 1086
BstSLI GKGCMC 1 cut(s) 866
BstV1I GCAGC 3 cut(s) 383, 503, 902
BstV2I GAAGAC 2 cut(s) 249, 696
BstX2I RGATCY 2 cut(s) 211, 1213
BstYI RGATCY 2 cut(s) 211, 1213
Bsu15I ATCGAT 1 cut(s) 486
BsuRI GGCC 3 cut(s) 546, 659, 1232
BsuTUI ATCGAT 1 cut(s) 486
BtgI CCRYGG 2 cut(s) 973, 1115
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 5 cut(s) 131, 170, 568, 643, 691
BtsI GCAGTG 2 cut(s) 52, 501
BtsIMutI CAGTG 5 cut(s) 52, 456, 501, 864, 1055
Cac8I GCNNGC 2 cut(s) 585, 1017
CfoI GCGC 4 cut(s) 396, 939, 1021, 1114
Cfr13I GGNCC 3 cut(s) 658, 970, 1231
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 1 cut(s) 442
CviAII CATG 7 cut(s) 593, 746, 795, 885, 907, 1022, 1116
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 5 cut(s) 245, 555, 579, 998, 1047
DinI GGCGCC 1 cut(s) 1113
DpnI GATC 7 cut(s) 195, 213, 291, 822, 1031, 1055, 1215
DpnII GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
DraIII CACNNNGTG 1 cut(s) 47
EaeI YGGCCR 1 cut(s) 544
Eco130I CCWWGG 2 cut(s) 437, 1115
Eco47I GGWCC 1 cut(s) 970
EcoRII CCWGG 6 cut(s) 358, 567, 693, 766, 932, 1086
EcoT14I CCWWGG 2 cut(s) 437, 1115
EgeI GGCGCC 1 cut(s) 1113
EheI GGCGCC 1 cut(s) 1113
ErhI CCWWGG 2 cut(s) 437, 1115
FaeI CATG 7 cut(s) 596, 749, 798, 888, 910, 1025, 1119
FatI CATG 7 cut(s) 592, 745, 794, 884, 906, 1021, 1115
FauI CCCGC 1 cut(s) 43
FauNDI CATATG 1 cut(s) 951
Fnu4HI GCNGC 3 cut(s) 372, 492, 891
FokI GGATG 5 cut(s) 118, 157, 575, 630, 698
Fsp4HI GCNGC 3 cut(s) 372, 492, 891
FspBI CTAG 2 cut(s) 414, 734
FspI TGCGCA 2 cut(s) 395, 1020
GlaI GCGC 4 cut(s) 395, 938, 1020, 1113
GluI GCNGC 3 cut(s) 372, 492, 891
GsaI CCCAGC 1 cut(s) 982
GsuI CTGGAG 3 cut(s) 214, 590, 897
HaeII RGCGCY 2 cut(s) 940, 1115
HaeIII GGCC 3 cut(s) 546, 659, 1232
HapII CCGG 1 cut(s) 854
HhaI GCGC 4 cut(s) 396, 939, 1021, 1114
Hin1I GRCGYC 1 cut(s) 1112
Hin1II CATG 7 cut(s) 596, 749, 798, 888, 910, 1025, 1119
Hin6I GCGC 4 cut(s) 394, 937, 1019, 1112
HinP1I GCGC 4 cut(s) 394, 937, 1019, 1112
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 8 cut(s) 33, 163, 377, 469, 523, 955, 1043, 1094
HpaII CCGG 1 cut(s) 854
HphI GGTGA 1 cut(s) 1103
Hpy166II GTNNAC 3 cut(s) 832, 864, 973
Hpy188III TCNNGA 5 cut(s) 121, 287, 647, 824, 986
Hpy8I GTNNAC 3 cut(s) 832, 864, 973
HpyAV CCTTC 2 cut(s) 188, 1083
HpyCH4III ACNGT 2 cut(s) 460, 1069
HpyCH4IV ACGT 1 cut(s) 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 640, 1187
HpyF3I CTNAG 5 cut(s) 245, 555, 579, 998, 1047
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 1112
Hsp92II CATG 7 cut(s) 596, 749, 798, 888, 910, 1025, 1119
HspAI GCGC 4 cut(s) 394, 937, 1019, 1112
KasI GGCGCC 1 cut(s) 1111
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
LmnI GCTCC 2 cut(s) 916, 1083
Lsp1109I GCAGC 3 cut(s) 383, 503, 902
LweI GCATC 3 cut(s) 140, 478, 652
MaeI CTAG 2 cut(s) 414, 734
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 4 cut(s) 88, 147, 424, 448
MalI GATC 7 cut(s) 195, 213, 291, 822, 1031, 1055, 1215
MboI GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
MboII GAAGA 3 cut(s) 249, 701, 731
MflI RGATCY 2 cut(s) 211, 1213
MhlI GDGCHC 1 cut(s) 866
MlsI TGGCCA 1 cut(s) 546
MluCI AATT 2 cut(s) 363, 712
MluNI TGGCCA 1 cut(s) 546
Mly113I GGCGCC 1 cut(s) 1112
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 164, 209
Mox20I TGGCCA 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 37
MscI TGGCCA 1 cut(s) 546
MseI TTAA 2 cut(s) 711, 791
MslI CAYNNNNRTG 2 cut(s) 540, 597
Msp20I TGGCCA 1 cut(s) 546
MspI CCGG 1 cut(s) 854
MspR9I CCNGG 7 cut(s) 360, 569, 695, 768, 855, 934, 1088
MvaI CCWGG 6 cut(s) 360, 569, 695, 768, 934, 1088
MwoI GCNNNNNNNGC 2 cut(s) 640, 1187
NarI GGCGCC 1 cut(s) 1112
NciI CCSGG 1 cut(s) 855
NcoI CCATGG 1 cut(s) 1115
NdeI CATATG 1 cut(s) 951
NdeII GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
NlaIII CATG 7 cut(s) 596, 749, 798, 888, 910, 1025, 1119
NlaIV GGNNCC 7 cut(s) 324, 443, 660, 777, 912, 1085, 1113
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 4 cut(s) 88, 147, 424, 448
NsbI TGCGCA 2 cut(s) 395, 1020
NspI RCATGY 1 cut(s) 596
PciI ACATGT 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 7 cut(s) 33, 163, 469, 523, 955, 1043, 1094
PflFI GACNNNGTC 1 cut(s) 968
PkrI GCNGC 3 cut(s) 373, 493, 892
Ple19I CGATCG 1 cut(s) 823
PleI GAGTC 1 cut(s) 385
PluTI GGCGCC 1 cut(s) 1115
PpsI GAGTC 1 cut(s) 385
PscI ACATGT 1 cut(s) 592
Psp6I CCWGG 6 cut(s) 358, 567, 693, 766, 932, 1086
PspFI CCCAGC 1 cut(s) 978
PspGI CCWGG 6 cut(s) 358, 567, 693, 766, 932, 1086
PspN4I GGNNCC 7 cut(s) 324, 443, 660, 777, 912, 1085, 1113
PspPI GGNCC 3 cut(s) 658, 970, 1231
PsuI RGATCY 2 cut(s) 211, 1213
PsyI GACNNNGTC 1 cut(s) 968
PvuI CGATCG 1 cut(s) 823
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 2 cut(s) 540, 597
SaqAI TTAA 2 cut(s) 711, 791
SatI GCNGC 3 cut(s) 372, 492, 891
Sau3AI GATC 7 cut(s) 193, 211, 289, 820, 1029, 1053, 1213
Sau96I GGNCC 3 cut(s) 658, 970, 1231
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 7 cut(s) 360, 569, 695, 768, 855, 934, 1088
SduI GDGCHC 1 cut(s) 866
SfaNI GCATC 3 cut(s) 140, 478, 652
SfoI GGCGCC 1 cut(s) 1113
SinI GGWCC 1 cut(s) 970
SmiMI CAYNNNNRTG 2 cut(s) 540, 597
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 2 cut(s) 363, 712
SsiI CCGC 2 cut(s) 50, 634
SspDI GGCGCC 1 cut(s) 1111
SspMI CTAG 2 cut(s) 414, 734
StyD4I CCNGG 7 cut(s) 358, 567, 693, 766, 853, 932, 1086
StyI CCWWGG 2 cut(s) 437, 1115
TaaI ACNGT 2 cut(s) 460, 1069
TaiI ACGT 1 cut(s) 426
TaqI TCGA 1 cut(s) 486
TasI AATT 2 cut(s) 363, 712
TfiI GAWTC 7 cut(s) 33, 163, 469, 523, 955, 1043, 1094
Tru1I TTAA 2 cut(s) 711, 791
Tru9I TTAA 2 cut(s) 711, 791
TscAI CASTG 5 cut(s) 52, 463, 501, 871, 1055
TseFI GTSAC 4 cut(s) 88, 147, 424, 448
TseI GCWGC 3 cut(s) 371, 491, 890
Tsp45I GTSAC 4 cut(s) 88, 147, 424, 448
TspDTI ATGAA 4 cut(s) 524, 702, 1049, 1065
TspRI CASTG 5 cut(s) 52, 463, 501, 871, 1055
Tth111I GACNNNGTC 1 cut(s) 968
VneI GTGCAC 1 cut(s) 862
VpaK11BI GGWCC 1 cut(s) 970
XceI RCATGY 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 2 cut(s) 414, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.