Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008862

Physical Location & Seq

Genomic Coordinates Reverse (-)

7659960 .. 7664800

Gene Structure 4841 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1106900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3405 bp

ATGGGGAAGCAGAACTCGAGTTTGGTCTCCGATGCGGTGGAGGCCACGGTGTTCGTCGACACCAGCCTCGGCACCCACCTCGCCGTCGCCGTCGACCCCAACGCCACCGTTTCCGCACTCAAAAGGAAAATAGAACATGAACATCTATTGTGCTTTAATAGCATGGGGAGAATCGAGGTTGAAGCTCTAAAGGTAAAACGCAAAGGATTCTTTTATCACCTATCAGATTCCATGGTTGTTAGAAATGCTTTCAGGGTCAAGATGAATTGGTTTGTTTCTGCCGATGCTAAAGGTGTTGCGGGGAAAAGATTGTCCAATTTTAATGATTCAAGGCCTTTGCTACTTGAGGGGAGAAAACGTGCAGAGGAGAAAGTTACTACTGGCGACCAGAATGGTTCAGTTGATTTTGCTGGAGAAACTTCAAAACAATTGGATATAAGGTTCAAGAGTTCTGATAATGATCATTGCACAATTTCATTTCATGAGACAACCAATCGTCTGGAACCTGAAATACAGGAGGACCACAGCTTACATAGCAAGGGATACATAGTTCCCAAAACACAAGAATGCAAGGAAGATGGTTCAAGGAAAGAAAGTGATGTCCAATGCAACGTCTCTTCGGAAAAAGCTTTTCCTGCTGAAAAGAAAAGACAACGCAAGACTAAGCAGAGAAATGAAAAAACAGTGCAGTTAAAAGAAGACAGCGTTTCAGTTCTTGGATTTGGTAAAGAACAGTCACAGACAGATATTGTACGTCCTGAAAATTCATCAGGCAATCAGAGTAGAGATGTTATATGTGATGTGACTATTGGAAAGAATACTACTACCAGTAAAGATGTTTCACAGGATATGGCGGCTGGAAACAGAAACACTACTGAAGAACCTTGCAGGAGTTTGGCTTCTAGTATGGATAACAGGTCAGATTCTGGAATTCGAAGGACTACTGTTGCATACAAGCACACTGAAGTTCTTGGAAAACACATTGAGAGCAGGGAGAATACATCTAAGCATGGCAGTCTTGCGGTCTCCGATACAGAAGTGGACGATGCTTCACTATCTCAGCCTGCTGTAAAGAACAAGCGGAAGCTTGCAAACATATCTCGTCTTGAGGATTCACTGAAAGAAAATGATAAAGTGACCTCCAAGCCAGAGATTGCATCTGAATTCTCTTTGAGGGACAAACAGAAAAATGCAAGTTCTATCTTAGGTGGTTTAACTGCCGAATCTTCGGATTTGATGACCAATAGTTCCAGAAAGAAGAAGAAAAATAGAAAGATTTCACCAAATTCTCTTGATCAAGTAGTTACTGCAGCTCTTTCCGGAAGAGATGTTATAGAAGAGACATCTGCTGCTGCTGCTTCAGGAAGTAATGATAAAAATTCAGGTGGAGAAGACAATGTTGCTTCTAATAATGAACAAGGTATACGTGGTGTAACGCCACATAAACTTAATGGAATCTCTCAGAAGGGAAATCACTGTGCTCCTGTCCAAGAGTTGCGGGAAAATTCTGGAGTATCCTCTTCACTAGATGGTAATAATACTGCCGATGCTGGCAATGTGGAAAGCAAAAGTGTTGCAGCTGAAGAAAAATGGGACGGCCATCCTCCCCCCGTCTTCAAAGTTCCCAGTGAAGGTGTAACTGATTCTGCTCAAAGAGTTGGAGAGGGGAGAGAATCATTACAGAGAAAGGATTCTGAATTGGTGAAATCTGAAAAGAAACAGTTGCCTGGTCAGGGTAAGAAAGATAGGAATGATACAAAAGTAATTGTGACATCTGAGTTGTTGGATGCGAATGGAATTAACACCCCTAATAACACCGTTAAGAAGAAAAAGAAAACCAGAAAAACCAAGGGTTCTATTGGAGAAACTTCGGTTGAATCAGGTGCAGAACACTTTAAAGGGTCTGAGAATGGACTTTCGTTGGATGAACCTCATGAGGAGGAAAGTAATATTTCTAAGAAAAATGGGAAAGATGTATCAGAAAAAGAAACAGAGGGTTCTCATATCGTTATGAAAACTGATAAACTTGGTCACAGTGAGGTGGGGACCCTGGAGCAAAATGGCAAACCTCAAGAAAATGAAGAAAGTATGGAGCAGAATGTCAAAAAGAAATCTAAGAGGAAAAGGAGTGCCATGACAAAAGACATCCCAAATTTGCAAGCTGAAGCGCAAAATGTTGGTCACCAGGTTCCAGCACCGACAACTGATAAGTTGGAAAAAGGAACTCCCATTGAACAATCAAATGGGAAAGCTCTGGAATCTGATGAGGGTTATGGCATGGAAACCGATTCAGTCGCTGCTCGGTCAAAATCCAAGCGTATTGAGCCTACTAAAGTTCATTCTCATGCACTAGAACACATTTCCGATGGGAGGGCTCTTGAAGCTAGCGCAACTGGTAACCCTAATGGATGCTCTAATGAGGTTGATAAGAACACGGAAGTTTCCTGTGAAGGCAATAAGGTCAACTTTGACAAGCATCAGATGCCTAGCCTGGGCCAGCATGAAGTTTCTGTCTCTGGAGAAGTGCACACCGAGGTGACTAGAACAAACATAACAGATGATAAGACAAAAAAGAAGCGAAAAAAGCTTGATGTGCAAAGTGTTCCTTCGCCTGATCTGCATAGTTCGCTCAAGTCTAATGAGAACCAAGGGATTGATGGAAGGTCTGAAGCTGGCAACGCTAGCTCCATACAACCGCAAGGATCATCATCCAGGGGTGACGACCTTATGCCTCACCCTCAAAAGAAGGGTTCGAAGAAAGTTCCTAAAACTGGGACCAAAACCCCAACACCTGACAAGTCTGATAAGATGAATTCCATTTCTCAAGCAGCTGGAAAGCACAGTGGTGCCAAAGTTTCTGGGACCGATATTCGTACGGGGAAAAAAAATGATTCTTCAGCTATATTGAATTCTAAATTGGACAAATCAAACAGTAAACCTCACCAAAATAAAATGGATAACAAGCGTCAATCAGGTGTCAACCGAAATCGTGTGGCCGGTGGGAAACCTTATGGTATTGATTATGGGGAAGTTGTAAACAGCCCAGAAAAGAGGAAGAGCTCAACTACACCAAGGAAAATTTTCATGGATGACGACAGTAGTGGGAGTTCTGAAGATGAAGATAAAGAACTTTCAAAAGCCAGCACCCAAACTCCATCAGATTATTCGTCATCTGGCTACTCAGATGGGGAAAGCAATGCAGATATGAACACGCCAAGAGGCGGAAGGGCTGGTGGGAGAAGCGTCATAAAATCATGCACTTCCGGCATGAAGGACATGAATTGGGATGAATTGGCTAAAAGTTCAAGCACATTCAAGAAGGCGAAACTTACGGCGTCTCAGTCACAACTGGAAGAGGACAGCGAGCGCGTTGAATTCGTTCCCGACAGCCAGCCTATATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006403 GO:0006464 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009987 GO:0015931 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0033036 GO:0033227 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050657 GO:0050658 GO:0051179 GO:0051234 GO:0051236 GO:0061630 GO:0061659 GO:0070647 GO:0071702 GO:0071704 GO:0071705 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

DUF4414 DUF908 DUF913 HECT UBA WWE

Protein Analysis

1135

Amino Acids

123.14

Weight (kDa)

8.48

Isoelectric Point (pI)

43.36

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008862)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25060 FvH4_4g25060 FvH4_4g25060 FvH4_4g25060
malus_domestica	MD13G1106900.v1.1 MD16G1107000.v1.1
prunus_persica	Prupe.1G239400_v2.0.a1 Prupe.1G239400_v2.0.a1
pyrus_communis	pycom13g09270 pycom16g09110
rosa_chinensis	RchiOBHm_Chr4g0432241
rosa_laevigata	RLG00000006860
rosa_multiflora	Rmu_co7961612.1_g000001 Rmu_sc0001084.1_g000049 Rmu_sc0039253.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373690
rosa_rugosa	Rorug04G0255400 Rorug04G0255500
rosa_samantha	Rh4AG312600 Rh4BG320100 Rh4CG335300 Rh4DG315500
rosa_wichuraiana	Rw4G027170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	71, 2846
AccI	GTMKAC	3 cut(s)	57, 93, 1423
AccII	CGCG	1 cut(s)	3369
AccIII	TCCGGA	1 cut(s)	1319
AciI	CCGC	9 cut(s)	35, 114, 299, 854, 1022, 1081, 1498, 2696, 3222
AclWI	GGATC	1 cut(s)	2710
AcoI	YGGCCR	2 cut(s)	1597, 2994
AcuI	CTGAAG	8 cut(s)	897, 984, 1344, 1602, 2184, 2688, 2880, 3132
AcyI	GRCGYC	1 cut(s)	3335
AfaI	GTAC	2 cut(s)	753, 2875
AfiI	CCNNNNNNNGG	6 cut(s)	1631, 1733, 2370, 2492, 2771, 3221
AjnI	CCWGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
AjuI	GAANNNNNNNTTGG	3 cut(s)	37, 2900, 2932
AleI	CACNNNNGTG	2 cut(s)	2534, 2844
AloI	GAACNNNNNNTCC	4 cut(s)	425, 457, 534, 566
Alw21I	GWGCWC	3 cut(s)	1483, 2529, 3062
Alw26I	GTCTC	7 cut(s)	31, 479, 619, 1030, 1334, 2518, 3342
Alw44I	GTGCAC	1 cut(s)	2525
AlwI	GGATC	1 cut(s)	2710
AlwNI	CAGNNNCTG	2 cut(s)	926, 2297
Ama87I	CYCGRG	1 cut(s)	16
Aor13HI	TCCGGA	1 cut(s)	1319
AoxI	GGCC	5 cut(s)	42, 332, 1597, 2494, 2994
ApaLI	GTGCAC	1 cut(s)	2525
ApeKI	GCWGC	7 cut(s)	1310, 1349, 1352, 1355, 1577, 2297, 2828
ArsI	GACNNNNNNTTYG	2 cut(s)	2164, 2196
Asp700I	GAANNNNTTC	4 cut(s)	1276, 1690, 3080, 3378
AspLEI	GCGC	3 cut(s)	2170, 2390, 3369
AspS9I	GGNCC	5 cut(s)	520, 2046, 2494, 2775, 2862
AsuHPI	GGTGA	8 cut(s)	209, 1272, 1714, 2174, 2548, 2726, 2729, 2933
AsuII	TTCGAA	2 cut(s)	934, 2753
AsuNHI	GCTAGC	2 cut(s)	2384, 2681
AvaI	CYCGRG	1 cut(s)	16
AvaII	GGWCC	4 cut(s)	520, 2046, 2775, 2862
BaeGI	GKGCMC	1 cut(s)	2529
BaeI	ACNNNNGTAYC	4 cut(s)	1023, 1056, 1746, 1779
BanI	GGYRCC	2 cut(s)	71, 2846
BanII	GRGCYC	2 cut(s)	2377, 3062
BbsI	GAAGAC	3 cut(s)	705, 1398, 1606
Bbv12I	GWGCWC	3 cut(s)	1483, 2529, 3062
BbvI	GCAGC	7 cut(s)	1322, 1336, 1339, 1342, 1589, 2284, 2840
BccI	CCATC	7 cut(s)	572, 1523, 1608, 2360, 2651, 3163, 3179
BceAI	ACGGC	4 cut(s)	68, 74, 1612, 3348
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
BciVI	GTATCC	2 cut(s)	536, 1525
BclI	TGATCA	2 cut(s)	460, 1294
BcoDI	GTCTC	7 cut(s)	31, 479, 619, 1030, 1334, 2518, 3342
BfaI	CTAG	7 cut(s)	903, 1526, 2351, 2385, 2487, 2541, 2682
BfmI	CTRYAG	1 cut(s)	1308
BfuI	GTATCC	2 cut(s)	536, 1525
BisI	GCNGC	8 cut(s)	855, 1311, 1350, 1353, 1356, 1578, 2298, 2829
BlsI	GCNGC	8 cut(s)	856, 1312, 1351, 1354, 1357, 1579, 2299, 2830
Bme1390I	CCNGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
Bme18I	GGWCC	4 cut(s)	520, 2046, 2775, 2862
BmeT110I	CYCGRG	1 cut(s)	16
BmgT120I	GGNCC	5 cut(s)	520, 2046, 2494, 2775, 2862
BmiI	GGNNCC	8 cut(s)	73, 504, 2047, 2048, 2190, 2776, 2848, 2863
BmrFI	CCNGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
BmrI	ACTGGG	2 cut(s)	1620, 2781
BmsI	GCATC	9 cut(s)	22, 274, 1036, 1166, 1537, 1777, 2399, 2472, 2485
BmtI	GCTAGC	2 cut(s)	2388, 2685
BmuI	ACTGGG	2 cut(s)	1620, 2781
BpiI	GAAGAC	3 cut(s)	705, 1398, 1606
BpmI	CTGGAG	4 cut(s)	432, 1530, 2072, 2538
Bpu14I	TTCGAA	2 cut(s)	934, 2753
BpuEI	CTTGAG	5 cut(s)	365, 1127, 2055, 2615, 2808
BsaAI	YACGTR	1 cut(s)	1427
BsaBI	GATNNNNATC	2 cut(s)	459, 2707
BsaHI	GRCGYC	1 cut(s)	3335
BsaI	GGTCTC	2 cut(s)	31, 1030
BsaWI	WCCGGW	1 cut(s)	1319
BsaXI	ACNNNNNCTCC	6 cut(s)	343, 373, 2669, 2699, 3136, 3166
Bsc4I	CCNNNNNNNGG	6 cut(s)	1631, 1733, 2370, 2492, 2771, 3221
Bse118I	RCCGGY	1 cut(s)	2996
Bse1I	ACTGG	6 cut(s)	385, 828, 1626, 2398, 2776, 3354
Bse3DI	GCAATG	3 cut(s)	463, 1561, 3202
Bse8I	GATNNNNATC	2 cut(s)	459, 2707
BseAI	TCCGGA	1 cut(s)	1319
BseBI	CCWGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
BseGI	GGATG	8 cut(s)	1600, 1792, 1930, 2145, 2414, 2708, 3094, 3292
BseJI	GATNNNNATC	2 cut(s)	459, 2707
BseLI	CCNNNNNNNGG	6 cut(s)	1631, 1733, 2370, 2492, 2771, 3221
BseMI	GCAATG	3 cut(s)	463, 1561, 3202
BseMII	CTCAG	6 cut(s)	1073, 1475, 1767, 1896, 3195, 3353
BseNI	ACTGG	6 cut(s)	385, 828, 1626, 2398, 2776, 3354
BseRI	GAGGAG	2 cut(s)	380, 1952
BseSI	GKGCMC	1 cut(s)	2529
BseXI	GCAGC	7 cut(s)	1322, 1336, 1339, 1342, 1589, 2284, 2840
BsgI	GTGCAG	3 cut(s)	381, 707, 1905
Bsh1236I	CGCG	1 cut(s)	3369
BshFI	GGCC	5 cut(s)	44, 334, 1599, 2496, 2996
BshNI	GGYRCC	2 cut(s)	71, 2846
BsiHKAI	GWGCWC	3 cut(s)	1483, 2529, 3062
BsiHKCI	CYCGRG	1 cut(s)	16
BsiSI	CCGG	3 cut(s)	1320, 2997, 3264
BsiWI	CGTACG	1 cut(s)	2873
BslFI	GGGAC	5 cut(s)	1190, 1607, 2059, 2788, 2875
BslI	CCNNNNNNNGG	6 cut(s)	1631, 1733, 2370, 2492, 2771, 3221
BsmAI	GTCTC	7 cut(s)	31, 479, 619, 1030, 1334, 2518, 3342
BsmBI	CGTCTC	2 cut(s)	619, 3342
BsmFI	GGGAC	5 cut(s)	1190, 1607, 2059, 2788, 2875
BsmI	GAATGC	1 cut(s)	572
BsnI	GGCC	5 cut(s)	44, 334, 1599, 2496, 2996
Bso31I	GGTCTC	2 cut(s)	31, 1030
BsoBI	CYCGRG	1 cut(s)	16
Bsp119I	TTCGAA	2 cut(s)	934, 2753
Bsp1286I	GDGCHC	4 cut(s)	1483, 2377, 2529, 3062
Bsp13I	TCCGGA	1 cut(s)	1319
Bsp143I	GATC	4 cut(s)	460, 1294, 2614, 2702
Bsp19I	CCATGG	1 cut(s)	231
BspACI	CCGC	9 cut(s)	35, 114, 299, 854, 1022, 1081, 1498, 2696, 3222
BspANI	GGCC	5 cut(s)	44, 334, 1599, 2496, 2996
BspCNI	CTCAG	6 cut(s)	1072, 1474, 1768, 1897, 3194, 3352
BspEI	TCCGGA	1 cut(s)	1319
BspFNI	CGCG	1 cut(s)	3369
BspHI	TCATGA	2 cut(s)	481, 1933
BspLI	GGNNCC	8 cut(s)	73, 504, 2047, 2048, 2190, 2776, 2848, 2863
BspMAI	CTGCAG	1 cut(s)	1312
BspOI	GCTAGC	2 cut(s)	2388, 2685
BspPI	GGATC	1 cut(s)	2710
BspQI	GCTCTTC	1 cut(s)	3050
BspT104I	TTCGAA	2 cut(s)	934, 2753
BspT107I	GGYRCC	2 cut(s)	71, 2846
BspTNI	GGTCTC	2 cut(s)	31, 1030
BsrDI	GCAATG	3 cut(s)	463, 1561, 3202
BsrFI	RCCGGY	1 cut(s)	2996
BsrI	ACTGG	6 cut(s)	385, 828, 1626, 2398, 2776, 3354
BssAI	RCCGGY	1 cut(s)	2996
BssMI	GATC	4 cut(s)	460, 1294, 2614, 2702
BssNAI	GTATAC	1 cut(s)	1424
BssNI	GRCGYC	1 cut(s)	3335
BssT1I	CCWWGG	4 cut(s)	231, 1848, 2647, 3071
Bst1107I	GTATAC	1 cut(s)	1424
Bst2UI	CCWGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
Bst6I	CTCTTC	6 cut(s)	622, 1318, 1332, 1525, 3050, 3348
BstACI	GRCGYC	1 cut(s)	3335
BstAPI	GCANNNNNTGC	1 cut(s)	2482
BstBAI	YACGTR	1 cut(s)	1427
BstBI	TTCGAA	2 cut(s)	934, 2753
BstDSI	CCRYGG	2 cut(s)	45, 231
BstEII	GGTNACC	2 cut(s)	2180, 2396
BstF5I	GGATG	8 cut(s)	1600, 1792, 1930, 2145, 2414, 2708, 3094, 3292
BstFNI	CGCG	1 cut(s)	3369
BstHHI	GCGC	3 cut(s)	2170, 2390, 3369
BstKTI	GATC	4 cut(s)	463, 1297, 2617, 2705
BstMAI	GTCTC	7 cut(s)	31, 479, 619, 1030, 1334, 2518, 3342
BstMBI	GATC	4 cut(s)	460, 1294, 2614, 2702
BstNI	CCWGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
BstPI	GGTNACC	2 cut(s)	2180, 2396
BstSCI	CCNGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
BstSFI	CTRYAG	1 cut(s)	1308
BstSLI	GKGCMC	1 cut(s)	2529
BstUI	CGCG	1 cut(s)	3369
BstV1I	GCAGC	7 cut(s)	1322, 1336, 1339, 1342, 1589, 2284, 2840
BstV2I	GAAGAC	3 cut(s)	705, 1398, 1606
BstXI	CCANNNNNNTGG	1 cut(s)	499
BstZ17I	GTATAC	1 cut(s)	1424
BsuI	GTATCC	2 cut(s)	536, 1525
BsuRI	GGCC	5 cut(s)	44, 334, 1599, 2496, 2996
BtgI	CCRYGG	2 cut(s)	45, 231
BtsCI	GGATG	8 cut(s)	1600, 1792, 1930, 2145, 2414, 2708, 3094, 3292
BtsIMutI	CAGTG	7 cut(s)	690, 960, 1115, 1474, 1633, 2041, 2848
CaiI	CAGNNNCTG	2 cut(s)	926, 2297
CciI	TCATGA	2 cut(s)	481, 1933
CfoI	GCGC	3 cut(s)	2170, 2390, 3369
Cfr10I	RCCGGY	1 cut(s)	2996
Cfr13I	GGNCC	5 cut(s)	520, 2046, 2494, 2775, 2862
CseI	GACGC	3 cut(s)	2954, 3232, 3324
CsiI	ACCWGGT	1 cut(s)	2184
Csp6I	GTAC	2 cut(s)	752, 2874
CviQI	GTAC	2 cut(s)	752, 2874
DpnI	GATC	4 cut(s)	462, 1296, 2616, 2704
DpnII	GATC	4 cut(s)	460, 1294, 2614, 2702
DraI	TTTAAA	1 cut(s)	1897
EaeI	YGGCCR	2 cut(s)	1597, 2994
Eam1104I	CTCTTC	6 cut(s)	622, 1318, 1332, 1525, 3050, 3348
EarI	CTCTTC	6 cut(s)	622, 1318, 1332, 1525, 3050, 3348
EciI	GGCGGA	1 cut(s)	3237
Ecl136II	GAGCTC	1 cut(s)	3060
Eco130I	CCWWGG	4 cut(s)	231, 1848, 2647, 3071
Eco147I	AGGCCT	1 cut(s)	334
Eco24I	GRGCYC	2 cut(s)	2377, 3062
Eco31I	GGTCTC	2 cut(s)	31, 1030
Eco47I	GGWCC	4 cut(s)	520, 2046, 2775, 2862
Eco53kI	GAGCTC	1 cut(s)	3060
Eco57I	CTGAAG	8 cut(s)	897, 984, 1344, 1602, 2184, 2688, 2880, 3132
Eco88I	CYCGRG	1 cut(s)	16
Eco91I	GGTNACC	2 cut(s)	2180, 2396
EcoICRI	GAGCTC	1 cut(s)	3060
EcoO109I	RGGNCCY	1 cut(s)	2046
EcoO65I	GGTNACC	2 cut(s)	2180, 2396
EcoRI	GAATTC	5 cut(s)	930, 1163, 2812, 2908, 3374
EcoRII	CCWGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
EcoT14I	CCWWGG	4 cut(s)	231, 1848, 2647, 3071
EcoT38I	GRGCYC	2 cut(s)	2377, 3062
ErhI	CCWWGG	4 cut(s)	231, 1848, 2647, 3071
Esp3I	CGTCTC	2 cut(s)	619, 3342
FalI	AAGNNNNNCTT	4 cut(s)	2450, 2482, 2590, 2622
FaqI	GGGAC	5 cut(s)	1190, 1607, 2059, 2788, 2875
FauI	CCCGC	2 cut(s)	292, 1491
FbaI	TGATCA	2 cut(s)	460, 1294
FblI	GTMKAC	3 cut(s)	57, 93, 1423
Fnu4HI	GCNGC	8 cut(s)	855, 1311, 1350, 1353, 1356, 1578, 2298, 2829
FokI	GGATG	8 cut(s)	1587, 1799, 1937, 2132, 2421, 2695, 3101, 3299
FriOI	GRGCYC	2 cut(s)	2377, 3062
Fsp4HI	GCNGC	8 cut(s)	855, 1311, 1350, 1353, 1356, 1578, 2298, 2829
FspBI	CTAG	7 cut(s)	903, 1526, 2351, 2385, 2487, 2541, 2682
GlaI	GCGC	3 cut(s)	2169, 2389, 3368
GluI	GCNGC	8 cut(s)	855, 1311, 1350, 1353, 1356, 1578, 2298, 2829
GsuI	CTGGAG	4 cut(s)	432, 1530, 2072, 2538
HaeIII	GGCC	5 cut(s)	44, 334, 1599, 2496, 2996
HapII	CCGG	3 cut(s)	1320, 2997, 3264
HgaI	GACGC	3 cut(s)	2954, 3232, 3324
HhaI	GCGC	3 cut(s)	2170, 2390, 3369
Hin1I	GRCGYC	1 cut(s)	3335
Hin6I	GCGC	3 cut(s)	2168, 2388, 3367
HinP1I	GCGC	3 cut(s)	2168, 2388, 3367
HincII	GTYRAC	4 cut(s)	58, 94, 2464, 2980
HindII	GTYRAC	4 cut(s)	58, 94, 2464, 2980
HindIII	AAGCTT	3 cut(s)	627, 1085, 2585
HpaII	CCGG	3 cut(s)	1320, 2997, 3264
HphI	GGTGA	8 cut(s)	209, 1272, 1714, 2174, 2548, 2726, 2729, 2933
Hpy166II	GTNNAC	9 cut(s)	58, 94, 1042, 1424, 2464, 2527, 2936, 2980, 3037
Hpy8I	GTNNAC	9 cut(s)	58, 94, 1042, 1424, 2464, 2527, 2936, 2980, 3037
Hpy99I	CGWCG	3 cut(s)	59, 89, 95
HpyCH4IV	ACGT	4 cut(s)	358, 612, 754, 1426
HpySE526I	ACGT	4 cut(s)	358, 612, 754, 1426
Hsp92I	GRCGYC	1 cut(s)	3335
HspAI	GCGC	3 cut(s)	2168, 2388, 3367
KflI	GGGWCCC	1 cut(s)	2046
Kpn2I	TCCGGA	1 cut(s)	1319
Ksp22I	TGATCA	2 cut(s)	460, 1294
Kzo9I	GATC	4 cut(s)	460, 1294, 2614, 2702
LguI	GCTCTTC	1 cut(s)	3050
LmnI	GCTCC	4 cut(s)	1486, 2053, 2092, 2690
Lsp1109I	GCAGC	7 cut(s)	1322, 1336, 1339, 1342, 1589, 2284, 2840
LweI	GCATC	9 cut(s)	22, 274, 1036, 1166, 1537, 1777, 2399, 2472, 2485
MabI	ACCWGGT	1 cut(s)	2184
MaeI	CTAG	7 cut(s)	903, 1526, 2351, 2385, 2487, 2541, 2682
MaeII	ACGT	4 cut(s)	358, 612, 754, 1426
MalI	GATC	4 cut(s)	462, 1296, 2616, 2704
MboI	GATC	4 cut(s)	460, 1294, 2614, 2702
MfeI	CAATTG	1 cut(s)	428
MhlI	GDGCHC	4 cut(s)	1483, 2377, 2529, 3062
MmeI	TCCRAC	4 cut(s)	1639, 1764, 1902, 2193
MroI	TCCGGA	1 cut(s)	1319
MroXI	GAANNNNTTC	4 cut(s)	1276, 1690, 3080, 3378
MseI	TTAA	8 cut(s)	156, 321, 692, 1214, 1449, 1800, 1821, 1896
MslI	CAYNNNNRTG	4 cut(s)	565, 2343, 2534, 2844
MspA1I	CMGCKG	2 cut(s)	1580, 2831
MspI	CCGG	3 cut(s)	1320, 2997, 3264
MspR9I	CCNGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
MunI	CAATTG	1 cut(s)	428
Mva1269I	GAATGC	1 cut(s)	572
MvaI	CCWGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
MvnI	CGCG	1 cut(s)	3369
NcoI	CCATGG	1 cut(s)	231
NdeII	GATC	4 cut(s)	460, 1294, 2614, 2702
NheI	GCTAGC	2 cut(s)	2384, 2681
NlaIV	GGNNCC	8 cut(s)	73, 504, 2047, 2048, 2190, 2776, 2848, 2863
NmeAIII	GCCGAG	1 cut(s)	48
NmuCI	GTSAC	9 cut(s)	735, 802, 1135, 1768, 2030, 2180, 2536, 2717, 3342
NspV	TTCGAA	2 cut(s)	934, 2753
OliI	CACNNNNGTG	2 cut(s)	2534, 2844
PaeR7I	CTCGAG	1 cut(s)	16
PagI	TCATGA	2 cut(s)	481, 1933
PceI	AGGCCT	1 cut(s)	334
PciSI	GCTCTTC	1 cut(s)	3050
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PctI	GAATGC	1 cut(s)	572
PdmI	GAANNNNTTC	4 cut(s)	1276, 1690, 3080, 3378
Pfl23II	CGTACG	1 cut(s)	2873
PkrI	GCNGC	8 cut(s)	856, 1312, 1351, 1354, 1357, 1579, 2299, 2830
Ppu21I	YACGTR	1 cut(s)	1427
PpuMI	RGGWCCY	1 cut(s)	2046
Psp124BI	GAGCTC	1 cut(s)	3062
Psp5II	RGGWCCY	1 cut(s)	2046
Psp6I	CCWGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
PspEI	GGTNACC	2 cut(s)	2180, 2396
PspGI	CCWGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
PspLI	CGTACG	1 cut(s)	2873
PspN4I	GGNNCC	8 cut(s)	73, 504, 2047, 2048, 2190, 2776, 2848, 2863
PspPI	GGNCC	5 cut(s)	520, 2046, 2494, 2775, 2862
PspPPI	RGGWCCY	1 cut(s)	2046
PspXI	VCTCGAGB	1 cut(s)	16
PsrI	GAACNNNNNNTAC	6 cut(s)	495, 527, 1407, 1439, 3091, 3123
PstI	CTGCAG	1 cut(s)	1312
PstNI	CAGNNNCTG	2 cut(s)	926, 2297
PvuII	CAGCTG	2 cut(s)	1580, 2831
RsaI	GTAC	2 cut(s)	753, 2875
RsaNI	GTAC	2 cut(s)	752, 2874
RseI	CAYNNNNRTG	4 cut(s)	565, 2343, 2534, 2844
SacI	GAGCTC	1 cut(s)	3062
SalI	GTCGAC	2 cut(s)	56, 92
SapI	GCTCTTC	1 cut(s)	3050
SaqAI	TTAA	8 cut(s)	156, 321, 692, 1214, 1449, 1800, 1821, 1896
SatI	GCNGC	8 cut(s)	855, 1311, 1350, 1353, 1356, 1578, 2298, 2829
Sau3AI	GATC	4 cut(s)	460, 1294, 2614, 2702
Sau96I	GGNCC	5 cut(s)	520, 2046, 2494, 2775, 2862
ScrFI	CCNGG	5 cut(s)	1728, 2051, 2186, 2492, 2713
SduI	GDGCHC	4 cut(s)	1483, 2377, 2529, 3062
SexAI	ACCWGGT	1 cut(s)	2184
SfaNI	GCATC	9 cut(s)	22, 274, 1036, 1166, 1537, 1777, 2399, 2472, 2485
SfcI	CTRYAG	1 cut(s)	1308
Sfr274I	CTCGAG	1 cut(s)	16
SfuI	TTCGAA	2 cut(s)	934, 2753
SinI	GGWCC	4 cut(s)	520, 2046, 2775, 2862
SlaI	CTCGAG	1 cut(s)	16
SmiMI	CAYNNNNRTG	4 cut(s)	565, 2343, 2534, 2844
SmlI	CTYRAG	6 cut(s)	16, 344, 1106, 2070, 2630, 2823
SmoI	CTYRAG	6 cut(s)	16, 344, 1106, 2070, 2630, 2823
SseBI	AGGCCT	1 cut(s)	334
SsiI	CCGC	9 cut(s)	35, 114, 299, 854, 1022, 1081, 1498, 2696, 3222
SspI	AATATT	1 cut(s)	1951
SspMI	CTAG	7 cut(s)	903, 1526, 2351, 2385, 2487, 2541, 2682
SstI	GAGCTC	1 cut(s)	3062
StuI	AGGCCT	1 cut(s)	334
StyD4I	CCNGG	5 cut(s)	1726, 2049, 2184, 2490, 2711
StyI	CCWWGG	4 cut(s)	231, 1848, 2647, 3071
TaiI	ACGT	4 cut(s)	361, 615, 757, 1429
TaqI	TCGA	6 cut(s)	17, 57, 93, 174, 934, 2753
TaqII	GACCGA	2 cut(s)	2292, 2879
TauI	GCSGC	1 cut(s)	857
Tru1I	TTAA	8 cut(s)	156, 321, 692, 1214, 1449, 1800, 1821, 1896
Tru9I	TTAA	8 cut(s)	156, 321, 692, 1214, 1449, 1800, 1821, 1896
TscAI	CASTG	7 cut(s)	690, 967, 1122, 1481, 1633, 2041, 2848
TseFI	GTSAC	9 cut(s)	735, 802, 1135, 1768, 2030, 2180, 2536, 2717, 3342
TseI	GCWGC	7 cut(s)	1310, 1349, 1352, 1355, 1577, 2297, 2828
Tsp45I	GTSAC	9 cut(s)	735, 802, 1135, 1768, 2030, 2180, 2536, 2717, 3342
TspGWI	ACGGA	1 cut(s)	2450
TspRI	CASTG	7 cut(s)	690, 967, 1122, 1481, 1633, 2041, 2848
VneI	GTGCAC	1 cut(s)	2525
VpaK11BI	GGWCC	4 cut(s)	520, 2046, 2775, 2862
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2654
XhoI	CTCGAG	1 cut(s)	16
XmiI	GTMKAC	3 cut(s)	57, 93, 1423
XmnI	GAANNNNTTC	4 cut(s)	1276, 1690, 3080, 3378
XspI	CTAG	7 cut(s)	903, 1526, 2351, 2385, 2487, 2541, 2682

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.