Rorug04G0255500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008862
Physical Location & Seq
Forward (+)
42818718 .. 42820005
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0255500.1

Sequence Viewer

Length: 576 bp
ATGGGCCAGGCACTTCGCCGAGCAGCCGGGAAAGCCCGACCCGGTTCCAGCATCGAAACGACGTCGTCGTCGTCAGTACCCAAGACCATCGTCAATAAGCGGCCTCCGGTGACTCCCCCCGAACCGCCTCCTGAGATCACCAAACCCGGCGAGGAGGGTTTCGATTCCGATGGTGGTGCAGGAGCAGCAGCACCCAATGCTGAGAATCTGCTTGAAGAACGCGATCCGAAGTTCGATTCAATGCTTAATCAGATGGTGGGTCGGATCAAATCAAAGCCCGGAGGCAAACCTGAAATGGGTGAGGCACATGTGGTGGAGAAGTACAAGAGGCCACTACCGAAACTGAGGGATACGAAACCGGATTCCGGTAGGTTTGAGGAAAGAGCTGCTCCTCCGGGGACTCTGAACGTGGCGCAGCTGCGTCACATTATACTATTGCATCAGGGCAAGGCTGAGGATCACAATGGCCCAATGGAGGTGCAGAGAATTGCAGACAAGTTTCGGGTTGATGTTGCAGAGGTTAAGAGGATTGTGCAGTTTGTGTCACTGCCTCCTGAGGATACCAGTAAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

191

Amino Acids

20.77

Weight (kDa)

9.12

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 67
AatII GACGTC 1 cut(s) 65
AccII CGCG 1 cut(s) 222
AciI CCGC 2 cut(s) 100, 125
AclWI GGATC 3 cut(s) 218, 272, 465
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 2 cut(s) 78, 323
AfiI CCNNNNNNNGG 3 cut(s) 296, 365, 475
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 2 cut(s) 215, 240
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 2 cut(s) 386, 418
AluI AGCT 2 cut(s) 386, 418
AlwI GGATC 3 cut(s) 218, 272, 465
AoxI GGCC 4 cut(s) 4, 101, 329, 466
ApeKI GCWGC 6 cut(s) 23, 185, 188, 386, 415, 418
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 4, 467
AsuC2I CCSGG 5 cut(s) 28, 42, 147, 279, 396
AsuHPI GGTGA 3 cut(s) 121, 130, 311
AxyI CCTNAGG 1 cut(s) 555
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 6 cut(s) 35, 197, 200, 373, 405, 427
BccI CCATC 3 cut(s) 95, 164, 247
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 8
BciVI GTATCC 2 cut(s) 343, 553
BcnI CCSGG 5 cut(s) 28, 42, 147, 279, 396
BfuI GTATCC 2 cut(s) 343, 553
BisI GCNGC 7 cut(s) 24, 101, 186, 189, 387, 416, 419
BlsI GCNGC 7 cut(s) 25, 102, 187, 190, 388, 417, 420
Bme1390I CCNGG 6 cut(s) 8, 28, 42, 147, 279, 396
BmgT120I GGNCC 2 cut(s) 4, 467
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 6 cut(s) 8, 28, 42, 147, 279, 396
BmsI GCATC 2 cut(s) 60, 448
BoxI GACNNNNGTC 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 453
BpuMI CCSGG 5 cut(s) 28, 42, 147, 279, 396
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 395
BsaWI WCCGGW 3 cut(s) 106, 358, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 365, 475
Bse1I ACTGG 1 cut(s) 564
Bse21I CCTNAGG 1 cut(s) 555
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 395
BseLI CCNNNNNNNGG 3 cut(s) 296, 365, 475
BseMII CTCAG 5 cut(s) 123, 192, 335, 444, 546
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 2 cut(s) 167, 381
BseXI GCAGC 6 cut(s) 35, 197, 200, 373, 405, 427
BsgI GTGCAG 3 cut(s) 198, 500, 554
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 6, 103, 331, 468
BsiSI CCGG 8 cut(s) 27, 42, 107, 147, 279, 359, 366, 395
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 3 cut(s) 296, 365, 475
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 4 cut(s) 6, 103, 331, 468
Bsp143I GATC 4 cut(s) 135, 223, 264, 457
BspACI CCGC 2 cut(s) 100, 125
BspANI GGCC 4 cut(s) 6, 103, 331, 468
BspCNI CTCAG 5 cut(s) 124, 193, 336, 445, 547
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 3 cut(s) 218, 272, 465
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 4 cut(s) 135, 223, 264, 457
BssNI GRCGYC 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 573
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 197
BstDEI CTNAG 5 cut(s) 132, 201, 344, 453, 555
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 4 cut(s) 138, 226, 267, 460
BstMBI GATC 4 cut(s) 135, 223, 264, 457
BstMWI GCNNNNNNNGC 3 cut(s) 32, 185, 197
BstNI CCWGG 1 cut(s) 8
BstNSI RCATGY 1 cut(s) 311
BstPAI GACNNNNGTC 1 cut(s) 89
BstSCI CCNGG 6 cut(s) 6, 26, 40, 145, 277, 394
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 6 cut(s) 35, 197, 200, 373, 405, 427
Bsu36I CCTNAGG 1 cut(s) 555
BsuI GTATCC 2 cut(s) 343, 553
BsuRI GGCC 4 cut(s) 6, 103, 331, 468
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 1 cut(s) 545
CfoI GCGC 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 4, 467
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 77, 322
CviAII CATG 1 cut(s) 308
CviQI GTAC 2 cut(s) 77, 322
DdeI CTNAG 5 cut(s) 132, 201, 344, 453, 555
DpnI GATC 4 cut(s) 137, 225, 266, 459
DpnII GATC 4 cut(s) 135, 223, 264, 457
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
Eco81I CCTNAGG 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 311
FaiI YATR 2 cut(s) 309, 431
FaqI GGGAC 1 cut(s) 412
FatI CATG 1 cut(s) 307
Fnu4HI GCNGC 7 cut(s) 24, 101, 186, 189, 387, 416, 419
Fsp4HI GCNGC 7 cut(s) 24, 101, 186, 189, 387, 416, 419
GlaI GCGC 1 cut(s) 414
GluI GCNGC 7 cut(s) 24, 101, 186, 189, 387, 416, 419
HaeIII GGCC 4 cut(s) 6, 103, 331, 468
HapII CCGG 8 cut(s) 27, 42, 107, 147, 279, 359, 366, 395
HgaI GACGC 1 cut(s) 410
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HinfI GANTC 6 cut(s) 112, 164, 205, 236, 362, 400
HpaII CCGG 8 cut(s) 27, 42, 107, 147, 279, 359, 366, 395
HphI GGTGA 3 cut(s) 121, 130, 311
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 5 cut(s) 169, 228, 252, 264, 405
Hpy188III TCNNGA 2 cut(s) 131, 554
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 4 cut(s) 64, 67, 70, 73
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4IV ACGT 2 cut(s) 62, 408
HpyCH4V TGCA 6 cut(s) 179, 439, 481, 491, 515, 535
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 185, 197
HpyF3I CTNAG 5 cut(s) 132, 201, 344, 453, 555
HpySE526I ACGT 2 cut(s) 62, 408
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 413
Kzo9I GATC 4 cut(s) 135, 223, 264, 457
LmnI GCTCC 2 cut(s) 182, 394
Lsp1109I GCAGC 6 cut(s) 35, 197, 200, 373, 405, 427
LweI GCATC 2 cut(s) 60, 448
MaeII ACGT 2 cut(s) 62, 408
MaeIII GTNAC 3 cut(s) 109, 422, 543
MalI GATC 4 cut(s) 137, 225, 266, 459
MboI GATC 4 cut(s) 135, 223, 264, 457
MboII GAAGA 1 cut(s) 227
MluCI AATT 1 cut(s) 486
MlyI GAGTC 2 cut(s) 106, 394
MmeI TCCRAC 1 cut(s) 242
MseI TTAA 2 cut(s) 246, 522
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 8 cut(s) 27, 42, 107, 147, 279, 359, 366, 395
MspR9I CCNGG 6 cut(s) 8, 28, 42, 147, 279, 396
MvaI CCWGG 1 cut(s) 8
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 32, 185, 197
NciI CCSGG 5 cut(s) 28, 42, 147, 279, 396
NdeII GATC 4 cut(s) 135, 223, 264, 457
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 1 cut(s) 46
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 3 cut(s) 109, 422, 543
NspI RCATGY 1 cut(s) 311
PciI ACATGT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 68
PfeI GAWTC 4 cut(s) 164, 205, 236, 362
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 7 cut(s) 25, 102, 187, 190, 388, 417, 420
PleI GAGTC 2 cut(s) 106, 394
PpsI GAGTC 2 cut(s) 106, 394
PscI ACATGT 1 cut(s) 307
PshAI GACNNNNGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 2 cut(s) 4, 467
PsyI GACNNNGTC 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 2 cut(s) 78, 323
RsaNI GTAC 2 cut(s) 77, 322
SaqAI TTAA 2 cut(s) 246, 522
SatI GCNGC 7 cut(s) 24, 101, 186, 189, 387, 416, 419
Sau3AI GATC 4 cut(s) 135, 223, 264, 457
Sau96I GGNCC 2 cut(s) 4, 467
SchI GAGTC 2 cut(s) 106, 394
ScrFI CCNGG 6 cut(s) 8, 28, 42, 147, 279, 396
SetI ASST 8 cut(s) 65, 292, 374, 388, 411, 420, 480, 522
SfaNI GCATC 2 cut(s) 60, 448
Sse9I AATT 1 cut(s) 486
SsiI CCGC 2 cut(s) 100, 125
StyD4I CCNGG 6 cut(s) 6, 26, 40, 145, 277, 394
TaaI ACNGT 1 cut(s) 573
TaiI ACGT 2 cut(s) 65, 411
TaqI TCGA 3 cut(s) 54, 162, 234
TasI AATT 1 cut(s) 486
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 4 cut(s) 164, 205, 236, 362
Tru1I TTAA 2 cut(s) 246, 522
Tru9I TTAA 2 cut(s) 246, 522
TscAI CASTG 1 cut(s) 552
TseFI GTSAC 3 cut(s) 109, 422, 543
TseI GCWGC 6 cut(s) 23, 185, 188, 386, 415, 418
Tsp45I GTSAC 3 cut(s) 109, 422, 543
TspRI CASTG 1 cut(s) 552
Tth111I GACNNNGTC 1 cut(s) 64
XceI RCATGY 1 cut(s) 311
ZraI GACGTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.