Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008862

Physical Location & Seq

Genomic Coordinates Reverse (-)

7504566 .. 7509461

Gene Structure 4896 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1107000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3390 bp

ATGAGGAAGGAAAACTTGACTTTGGTCTCCCATACGACGGAGGCCACGGTGTTCGTCGACACCAGCCTCGGCACCCACATCGCCGTCACCATCGACCCCAACGACACCGTTTCTGCACTCAAACAGAAAATAGAACATGAACATCTATTGTGCTTTACTAGGGCGGGAAAAATCGAAGTTGAAGCTCTAAAGGTAAAACGCAAAGGATTCTTTTATCACCTATCAGATTCCATGGTTGTTAGAAATGCTTTCAGGGTCAACAATAACTGGTTTGTTTCTGCCGATGCTAAAGATGTTCCGGGGAAAAGATTGTCCAATTTTAATGATTCAATGCCATTGCTACTTGAGGGGAACAAGCGTGCAGAAGAGAAATTTAATATTGCTGACCGGGCTGGTTCAGTTGGTTCTGCTAGAGAAACTTCAAAACAATTGGAGATAGGGTTCAAGAGTTCTGGTATTGATCATTGTGCAACTTCATTTGATGAGACGGCCAATCAGCTGGAACCTGAAATACAGGAGGACCACAGCCTACATAGCGAGGGGTACAGAGTTCCCAAAACACAAGATTGCAGGGCAGATGGCTCGAGGAAAGAAATTGATGTCCAATGCGACGTCTCTTCGGAAAAAGCTTTGCCTGCTGAAAAGAAAAGACAACGCAAGACTAAGCAGAGAAAAGAAAAACCAGTGCAGTTAAAAGAAGACGGTGCTTCAGTTCTTGGATTTGGTAAAGAACAGTCGCAGCCAGATAATATACCACCTGAAAATTCATTAGGCAATCAGAGTAGAGATGTTCTATGTGACGTGACTTTTGGAAAGAAAACTACTACCAGTAAAGATGTTTCACAGGATATGGCAGCTGGAAACAAAAACACTACAGAAGAACCTTGCGGGAGTTCAGCTTCTAGTATGGATAACAGGTCACATTCTGGAATTCATATGACAAATGATGCGTACAAGGGCACTGAAGTTCTTGGAAAACACATCGGGAGCAGGGAGAATACATCTAAGCATGGCAGTTTTGTGGTCTCCAATATAGCAGTGGAAGATGCTTCACAATCTCAGCCTGCTGCAAAGAAAAAGCGGAGGTTTGAAAACATATCTGGTCTTGAGGATTCGCTGAAAGAAAATGATAAAGTGACTTCCAAGCCAGAGATTGCATCTGAATTCTCTTTAAGGGACACACAGAAAAATGCAAGTGCTATCTTATATGGTTCAACTGCCGAATCTTCGGAGAAGAAGAAGAAAAAGAGAAAAATTTCACCAAATTCTCTTCATGAAGTAGTTGCTGCAGCTCCTTCCGGAAGAGATGTTTTACAAGAGAGATCTGCTGTTGCTGCTTCAGGAAGTAATGATAAAAATTCAGGTGTAGAAGACAATGCTGCTGCTATTAATGAACAAGGTATACAAGCTTTAATGCCACCTAAACTCAATGGAATCTCTCAGAAGGGAAATCACTGTGCTCATGTCCAAGAGTTGCGGGAAAATTCTGGATTACCCTCATCACTGGATGTTAATAATACTGCCAATGTGGAAAACAAAAGTGGTGAGGCTGAAGAAGGATTGGACAACCGTCCTTCCTCTGTTTTCAAAGTTCCCAGTGAAGGTGTAATTGATTGCGTAGAAAGAGTTGGAGAGGAGAGAGAATCATTACAGAGAAAGGATTCTGAATTGGTGACGTCTGAAAAGACACAGTTGCCTGGTCAGGATAAGAGAGATAGGAATGATACAAAAGTAATTGTGAAATCTGAATGGTTCGATGCAAATGGAATGGTCACCCCTAATAACACTAGTAAGAAGAAAAAGAAGACCAGAAAAACCAAGGGATCCATAGGAGAAACTTCGGTTGAATCAGGTGCAGGACACATTAAAGGTTCTGAGAATGGAATTTCGTTGGATGAACCTCGTGAAGCTGTACATGGTGAACATGCTAGTGATAAAGCTAAGGAGGAGGCAAGTAATGTTTCTAAGAAAAATGGGATAGACGTATTAGAAAAAGAAACGGAGAGTTCTCATATCGTTGCGAAAACTGGTAGCCTTGATCTCAGTGAGGTGGGGAACCTGGAGCAAAATGGCAAACCTCAAGAAAATGCAGAAGGTATGGAGCAGAACATCAAAAAGAAATCTAAGAGGAAAAAGAGTACCATGACAAAAGAAATCCCGAATTTGAAAGCTGAAGCGCAAAAAGTTGGTCACCAGGTTCCAACACCGACAACTGATATGTTGGACAAAACAAGTCCCATTGAACAATCAAATGGGAAAGCTGTGGAATCTGATAAGGGCTATGGCATGGAAACTGATTCGGTCCCTGCTCGGTCAAAATCCAAGCGTATTGACCCTACTAAAGCTCCTTCTCATGCACTAGAACACACTAGCATAAGTTTTGGGAACCCTCCTGAAAATGGATGCTCTAATGAGGCTGATAAGAACATGGAAGTTTCTTGTGAAGGCAATAAGGACAACTTTGACAACCATCAGGTGCCTGGCCTAGGCCAGCATGAAGTTGCCACCTCTAAAGAAGTGCACGCCGAGGTAACTAGAACAAACAGAGCAGATGATAAACCAAAAACGAAGCGAAGGAAGCTTGATGTACAAAGTGGTCCCTCGCCTGATCTGCAAAGTTCGCTCAAGTCTAAGAAGAACCAAGGGATCAATGGAAGGTCTGAAGCTGGCAACTCTAACTCCATACAGCCGCAAGGATCATCATCCAAGGGTGACGATGGAAAAGGGTTTTCGAAGAAAGTTCCTAAAACTGGGACCAAAGCCCCAACACCGGATAAGTCTGATAAGATGAATTCCATTTCTAAAGCAGCCCGAACTCACAGTGGTGCTAACGTTTCTGGTATTGATGGTACTCGTATAGGGAAAAAGAATGATTCTTCAGCTGTACTGAATTCTAAATTGGAAAAAGCAAACAATAAACCTCACCAAAATAAAATGGATAACAAGTCTCAGTCAGGTGTGAACCGAAATCGTGTGGCCAGTGGAAAACCTTATGGTATCGATTATGGGGAAGTTGTAAACAGCCCAGAAAAGAGGAAGATCTTAGCTACGCCAAGGAAAATTTTCAAGGATGATGACAGTAGTGGGAGTTCTGAAGATGAAGGTAAAGATGAACTTTCAAAGGCCAGCACCCAAACTCCATCAGATTATTCGTCGTCTGGCTACTCAGATGGGGAAAGCAATGCAGATATGAGCACACCAAGAAGCGGAAAGGCTGGTGAGAGAAGCGTCATAAAATCCTGCACTTCCGGCATAAAGGACATGAATTGGGATGAATTGGCCAAAAGTTCAAGCACATTCAAGAAGGCAAAACTTACAGCGTCTCAGTCACAACTGGAGGAGGACAGCGAACCCATTGAACCCGTACCCGACAGTCAGCCTGTATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006403 GO:0006464 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009987 GO:0015931 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0033036 GO:0033227 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050657 GO:0050658 GO:0051179 GO:0051234 GO:0051236 GO:0061630 GO:0061659 GO:0070647 GO:0071702 GO:0071704 GO:0071705 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

DUF4414 DUF908 DUF913 HECT UBA WWE

Protein Analysis

1130

Amino Acids

122.33

Weight (kDa)

8.01

Isoelectric Point (pI)

44.06

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008862)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25060 FvH4_4g25060 FvH4_4g25060 FvH4_4g25060
malus_domestica	MD13G1106900.v1.1 MD16G1107000.v1.1
prunus_persica	Prupe.1G239400_v2.0.a1 Prupe.1G239400_v2.0.a1
pyrus_communis	pycom13g09270 pycom16g09110
rosa_chinensis	RchiOBHm_Chr4g0432241
rosa_laevigata	RLG00000006860
rosa_multiflora	Rmu_co7961612.1_g000001 Rmu_sc0001084.1_g000049 Rmu_sc0039253.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373690
rosa_rugosa	Rorug04G0255400 Rorug04G0255500
rosa_samantha	Rh4AG312600 Rh4BG320100 Rh4CG335300 Rh4DG315500
rosa_wichuraiana	Rw4G027170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	615, 1679
AccB1I	GGYRCC	2 cut(s)	71, 2476
AccI	GTMKAC	2 cut(s)	57, 1402
AccIII	TCCGGA	1 cut(s)	1298
AciI	CCGC	6 cut(s)	164, 888, 1081, 1477, 2690, 3207
AclI	AACGTT	1 cut(s)	2832
AclWI	GGATC	4 cut(s)	1818, 1831, 2654, 2704
AcoI	YGGCCR	3 cut(s)	489, 2976, 3279
AcuI	CTGAAG	8 cut(s)	693, 984, 1323, 1572, 2193, 2682, 2862, 3114
AcyI	GRCGYC	2 cut(s)	612, 1676
AfaI	GTAC	8 cut(s)	545, 953, 1914, 2140, 2589, 2851, 2886, 3366
AfiI	CCNNNNNNNGG	7 cut(s)	37, 1601, 2399, 2486, 2750, 2770, 3206
AhlI	ACTAGT	1 cut(s)	1787
AjiI	CACGTC	1 cut(s)	802
AjnI	CCWGG	4 cut(s)	1696, 2058, 2193, 2479
AjuI	GAANNNNNNNTTGG	2 cut(s)	2882, 2914
AleI	CACNNNNGTG	1 cut(s)	2823
AloI	GAACNNNNNNTCC	2 cut(s)	425, 457
Alw21I	GWGCWC	3 cut(s)	1462, 2523, 3197
Alw26I	GTCTC	6 cut(s)	31, 479, 619, 1030, 2952, 3327
Alw44I	GTGCAC	1 cut(s)	2519
AlwI	GGATC	4 cut(s)	1818, 1831, 2654, 2704
Ama87I	CYCGRG	1 cut(s)	583
Aor13HI	TCCGGA	1 cut(s)	1298
AoxI	GGCC	7 cut(s)	42, 489, 2482, 2488, 2976, 3123, 3279
ApaLI	GTGCAC	1 cut(s)	2519
ApeKI	GCWGC	9 cut(s)	739, 854, 1067, 1286, 1289, 1334, 1379, 1382, 2807
ArsI	GACNNNNNNTTYG	4 cut(s)	791, 823, 2173, 2205
AseI	ATTAAT	1 cut(s)	1389
Asp700I	GAANNNNTTC	3 cut(s)	1255, 1660, 3062
AspA2I	CCTAGG	1 cut(s)	2485
AspLEI	GCGC	1 cut(s)	2179
AspS9I	GGNCC	4 cut(s)	520, 2302, 2597, 2754
AsuC2I	CCSGG	2 cut(s)	300, 389
AsuII	TTCGAA	1 cut(s)	2732
AvaI	CYCGRG	1 cut(s)	583
AvaII	GGWCC	4 cut(s)	520, 2302, 2597, 2754
AvrII	CCTAGG	1 cut(s)	2485
BaeGI	GKGCMC	2 cut(s)	962, 2523
BaeI	ACNNNNGTAYC	2 cut(s)	1716, 1749
BalI	TGGCCA	2 cut(s)	2978, 3281
BamHI	GGATCC	1 cut(s)	1823
BanI	GGYRCC	2 cut(s)	71, 2476
BauI	CACGAG	1 cut(s)	1902
BbsI	GAAGAC	3 cut(s)	705, 1377, 1811
Bbv12I	GWGCWC	3 cut(s)	1462, 2523, 3197
BbvI	GCAGC	9 cut(s)	751, 866, 1054, 1273, 1301, 1321, 1366, 1369, 2819
BccI	CCATC	7 cut(s)	98, 572, 2478, 2711, 2840, 3148, 3164
BceAI	ACGGC	2 cut(s)	68, 504
BcgI	CGANNNNNNTGC	4 cut(s)	61, 95, 564, 598
BciT130I	CCWGG	4 cut(s)	1698, 2060, 2195, 2481
BclI	TGATCA	1 cut(s)	460
BcnI	CCSGG	2 cut(s)	300, 389
BcoDI	GTCTC	6 cut(s)	31, 479, 619, 1030, 2952, 3327
BcuI	ACTAGT	1 cut(s)	1787
BfaI	CTAG	9 cut(s)	159, 411, 903, 1788, 1929, 2360, 2370, 2486, 2535
BfmI	CTRYAG	2 cut(s)	873, 1287
BglII	AGATCT	2 cut(s)	1322, 3039
BlnI	CCTAGG	1 cut(s)	2485
Bme1390I	CCNGG	6 cut(s)	300, 389, 1698, 2060, 2195, 2481
Bme18I	GGWCC	4 cut(s)	520, 2302, 2597, 2754
BmeT110I	CYCGRG	1 cut(s)	583
BmgBI	CACGTC	1 cut(s)	802
BmgT120I	GGNCC	4 cut(s)	520, 2302, 2597, 2754
BmrFI	CCNGG	6 cut(s)	300, 389, 1698, 2060, 2195, 2481
BmrI	ACTGGG	2 cut(s)	1590, 2760
BmsI	GCATC	6 cut(s)	274, 937, 1036, 1166, 1747, 2393
BmuI	ACTGGG	2 cut(s)	1590, 2760
BoxI	GACNNNNGTC	2 cut(s)	23, 1569
BpiI	GAAGAC	3 cut(s)	705, 1377, 1811
BpmI	CTGGAG	2 cut(s)	2081, 3356
Bpu10I	CCTNAGC	1 cut(s)	1941
Bpu14I	TTCGAA	1 cut(s)	2732
BpuEI	CTTGAG	4 cut(s)	365, 1127, 2064, 2609
BpuMI	CCSGG	2 cut(s)	300, 389
Bsa29I	ATCGAT	1 cut(s)	3000
BsaBI	GATNNNNATC	1 cut(s)	2701
BsaHI	GRCGYC	2 cut(s)	612, 1676
BsaI	GGTCTC	2 cut(s)	31, 1030
BsaWI	WCCGGW	2 cut(s)	1298, 2770
BsaXI	ACNNNNNCTCC	8 cut(s)	425, 455, 2329, 2359, 2663, 2693, 3121, 3151
Bsc4I	CCNNNNNNNGG	7 cut(s)	37, 1601, 2399, 2486, 2750, 2770, 3206
Bse1I	ACTGG	9 cut(s)	272, 683, 828, 1509, 1596, 2032, 2755, 2979, 3339
Bse3DI	GCAATG	2 cut(s)	335, 3187
Bse8I	GATNNNNATC	1 cut(s)	2701
BseAI	TCCGGA	1 cut(s)	1298
BseBI	CCWGG	4 cut(s)	1698, 2060, 2195, 2481
BseCI	ATCGAT	1 cut(s)	3000
BseGI	GGATG	6 cut(s)	1513, 1900, 2408, 2702, 3076, 3277
BseJI	GATNNNNATC	1 cut(s)	2701
BseLI	CCNNNNNNNGG	7 cut(s)	37, 1601, 2399, 2486, 2750, 2770, 3206
BseMI	GCAATG	2 cut(s)	335, 3187
BseMII	CTCAG	7 cut(s)	1073, 1454, 1866, 2056, 2963, 3180, 3338
BseNI	ACTGG	9 cut(s)	272, 683, 828, 1509, 1596, 2032, 2755, 2979, 3339
BseRI	GAGGAG	3 cut(s)	1649, 1961, 3353
BseSI	GKGCMC	2 cut(s)	962, 2523
BseXI	GCAGC	9 cut(s)	751, 866, 1054, 1273, 1301, 1321, 1366, 1369, 2819
BsgI	GTGCAG	5 cut(s)	99, 381, 707, 1875, 3226
BshFI	GGCC	7 cut(s)	44, 491, 2484, 2490, 2978, 3125, 3281
BshNI	GGYRCC	2 cut(s)	71, 2476
BshVI	ATCGAT	1 cut(s)	3000
BsiHKAI	GWGCWC	3 cut(s)	1462, 2523, 3197
BsiHKCI	CYCGRG	1 cut(s)	583
BsiSI	CCGG	5 cut(s)	299, 388, 1299, 2771, 3249
BslFI	GGGAC	5 cut(s)	1190, 2220, 2288, 2583, 2767
BslI	CCNNNNNNNGG	7 cut(s)	37, 1601, 2399, 2486, 2750, 2770, 3206
BsmAI	GTCTC	6 cut(s)	31, 479, 619, 1030, 2952, 3327
BsmBI	CGTCTC	3 cut(s)	479, 619, 3327
BsmFI	GGGAC	5 cut(s)	1190, 2220, 2288, 2583, 2767
BsnI	GGCC	7 cut(s)	44, 491, 2484, 2490, 2978, 3125, 3281
Bso31I	GGTCTC	2 cut(s)	31, 1030
BsoBI	CYCGRG	1 cut(s)	583
Bsp119I	TTCGAA	1 cut(s)	2732
Bsp1286I	GDGCHC	4 cut(s)	962, 1462, 2523, 3197
Bsp13I	TCCGGA	1 cut(s)	1298
Bsp1407I	TGTACA	2 cut(s)	1912, 2587
Bsp143I	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
Bsp19I	CCATGG	1 cut(s)	231
BspACI	CCGC	6 cut(s)	164, 888, 1081, 1477, 2690, 3207
BspANI	GGCC	7 cut(s)	44, 491, 2484, 2490, 2978, 3125, 3281
BspCNI	CTCAG	7 cut(s)	1072, 1453, 1867, 2055, 2962, 3179, 3337
BspDI	ATCGAT	1 cut(s)	3000
BspEI	TCCGGA	1 cut(s)	1298
BspHI	TCATGA	1 cut(s)	1273
BspMAI	CTGCAG	1 cut(s)	1291
BspPI	GGATC	4 cut(s)	1818, 1831, 2654, 2704
BspT104I	TTCGAA	1 cut(s)	2732
BspT107I	GGYRCC	2 cut(s)	71, 2476
BspTNI	GGTCTC	2 cut(s)	31, 1030
BsrDI	GCAATG	2 cut(s)	335, 3187
BsrGI	TGTACA	2 cut(s)	1912, 2587
BsrI	ACTGG	9 cut(s)	272, 683, 828, 1509, 1596, 2032, 2755, 2979, 3339
BssMI	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
BssNAI	GTATAC	1 cut(s)	1403
BssNI	GRCGYC	2 cut(s)	612, 1676
BssSI	CACGAG	1 cut(s)	1902
BssT1I	CCWWGG	6 cut(s)	231, 1818, 2485, 2641, 2706, 3053
Bst1107I	GTATAC	1 cut(s)	1403
Bst2BI	CACGAG	1 cut(s)	1902
Bst2UI	CCWGG	4 cut(s)	1698, 2060, 2195, 2481
Bst6I	CTCTTC	4 cut(s)	360, 622, 1275, 1297
BstACI	GRCGYC	2 cut(s)	612, 1676
BstAUI	TGTACA	2 cut(s)	1912, 2587
BstBI	TTCGAA	1 cut(s)	2732
BstC8I	GCNNGC	7 cut(s)	360, 636, 1065, 2492, 2523, 2668, 3127
BstDSI	CCRYGG	2 cut(s)	45, 231
BstEII	GGTNACC	2 cut(s)	1771, 2189
BstENI	CCTNNNNNAGG	1 cut(s)	2484
BstF5I	GGATG	6 cut(s)	1513, 1900, 2408, 2702, 3076, 3277
BstHHI	GCGC	1 cut(s)	2179
BstKTI	GATC	8 cut(s)	463, 1325, 1826, 2041, 2611, 2649, 2699, 3042
BstMAI	GTCTC	6 cut(s)	31, 479, 619, 1030, 2952, 3327
BstMBI	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
BstMWI	GCNNNNNNNGC	8 cut(s)	389, 534, 635, 1334, 2578, 2611, 2620, 3249
BstNI	CCWGG	4 cut(s)	1698, 2060, 2195, 2481
BstNSI	RCATGY	1 cut(s)	1928
BstPAI	GACNNNNGTC	2 cut(s)	23, 1569
BstPI	GGTNACC	2 cut(s)	1771, 2189
BstSCI	CCNGG	6 cut(s)	298, 387, 1696, 2058, 2193, 2479
BstSFI	CTRYAG	2 cut(s)	873, 1287
BstSLI	GKGCMC	2 cut(s)	962, 2523
BstV1I	GCAGC	9 cut(s)	751, 866, 1054, 1273, 1301, 1321, 1366, 1369, 2819
BstV2I	GAAGAC	3 cut(s)	705, 1377, 1811
BstX2I	RGATCY	3 cut(s)	1322, 1823, 3039
BstXI	CCANNNNNNTGG	1 cut(s)	499
BstYI	RGATCY	3 cut(s)	1322, 1823, 3039
BstZ17I	GTATAC	1 cut(s)	1403
Bsu15I	ATCGAT	1 cut(s)	3000
BsuRI	GGCC	7 cut(s)	44, 491, 2484, 2490, 2978, 3125, 3281
BsuTUI	ATCGAT	1 cut(s)	3000
BtgI	CCRYGG	2 cut(s)	45, 231
BtgZI	GCGATG	1 cut(s)	64
BtrI	CACGTC	1 cut(s)	802
BtsCI	GGATG	6 cut(s)	1513, 1900, 2408, 2702, 3076, 3277
BtsI	GCAGTG	1 cut(s)	1044
BtsIMutI	CAGTG	9 cut(s)	690, 960, 1044, 1453, 1502, 1603, 2050, 2827, 2986
Cac8I	GCNNGC	7 cut(s)	360, 636, 1065, 2492, 2523, 2668, 3127
CciI	TCATGA	1 cut(s)	1273
CfoI	GCGC	1 cut(s)	2179
Cfr13I	GGNCC	4 cut(s)	520, 2302, 2597, 2754
ClaI	ATCGAT	1 cut(s)	3000
CseI	GACGC	2 cut(s)	3217, 3309
CsiI	ACCWGGT	1 cut(s)	2193
Csp6I	GTAC	8 cut(s)	544, 952, 1913, 2139, 2588, 2850, 2885, 3365
CviQI	GTAC	8 cut(s)	544, 952, 1913, 2139, 2588, 2850, 2885, 3365
DpnI	GATC	8 cut(s)	462, 1324, 1825, 2040, 2610, 2648, 2698, 3041
DpnII	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
EaeI	YGGCCR	3 cut(s)	489, 2976, 3279
Eam1104I	CTCTTC	4 cut(s)	360, 622, 1275, 1297
EarI	CTCTTC	4 cut(s)	360, 622, 1275, 1297
Eco130I	CCWWGG	6 cut(s)	231, 1818, 2485, 2641, 2706, 3053
Eco31I	GGTCTC	2 cut(s)	31, 1030
Eco47I	GGWCC	4 cut(s)	520, 2302, 2597, 2754
Eco57I	CTGAAG	8 cut(s)	693, 984, 1323, 1572, 2193, 2682, 2862, 3114
Eco88I	CYCGRG	1 cut(s)	583
Eco91I	GGTNACC	2 cut(s)	1771, 2189
EcoNI	CCTNNNNNAGG	1 cut(s)	2484
EcoO65I	GGTNACC	2 cut(s)	1771, 2189
EcoRI	GAATTC	4 cut(s)	930, 1163, 2791, 2890
EcoRII	CCWGG	4 cut(s)	1696, 2058, 2193, 2479
EcoT14I	CCWWGG	6 cut(s)	231, 1818, 2485, 2641, 2706, 3053
ErhI	CCWWGG	6 cut(s)	231, 1818, 2485, 2641, 2706, 3053
Esp3I	CGTCTC	3 cut(s)	479, 619, 3327
FalI	AAGNNNNNCTT	5 cut(s)	31, 2444, 2476, 3099, 3131
FaqI	GGGAC	5 cut(s)	1190, 2220, 2288, 2583, 2767
FauI	CCCGC	3 cut(s)	157, 881, 1470
FauNDI	CATATG	1 cut(s)	936
FbaI	TGATCA	1 cut(s)	460
FblI	GTMKAC	2 cut(s)	57, 1402
FokI	GGATG	6 cut(s)	1520, 1907, 2415, 2689, 3083, 3284
FspBI	CTAG	9 cut(s)	159, 411, 903, 1788, 1929, 2360, 2370, 2486, 2535
GlaI	GCGC	1 cut(s)	2178
GsuI	CTGGAG	2 cut(s)	2081, 3356
HaeIII	GGCC	7 cut(s)	44, 491, 2484, 2490, 2978, 3125, 3281
HapII	CCGG	5 cut(s)	299, 388, 1299, 2771, 3249
HgaI	GACGC	2 cut(s)	3217, 3309
HhaI	GCGC	1 cut(s)	2179
Hin1I	GRCGYC	2 cut(s)	612, 1676
Hin6I	GCGC	1 cut(s)	2177
HinP1I	GCGC	1 cut(s)	2177
HincII	GTYRAC	2 cut(s)	58, 259
HindII	GTYRAC	2 cut(s)	58, 259
HindIII	AAGCTT	3 cut(s)	627, 1407, 2579
HpaII	CCGG	5 cut(s)	299, 388, 1299, 2771, 3249
Hpy166II	GTNNAC	7 cut(s)	58, 259, 1403, 1922, 2521, 2962, 3019
Hpy8I	GTNNAC	7 cut(s)	58, 259, 1403, 1922, 2521, 2962, 3019
Hpy99I	CGWCG	4 cut(s)	40, 59, 614, 3157
HpyCH4IV	ACGT	5 cut(s)	612, 801, 1676, 1983, 2832
HpyF10VI	GCNNNNNNNGC	8 cut(s)	389, 534, 635, 1334, 2578, 2611, 2620, 3249
HpySE526I	ACGT	5 cut(s)	612, 801, 1676, 1983, 2832
Hsp92I	GRCGYC	2 cut(s)	612, 1676
HspAI	GCGC	1 cut(s)	2177
Kpn2I	TCCGGA	1 cut(s)	1298
Ksp22I	TGATCA	1 cut(s)	460
Kzo9I	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
LmnI	GCTCC	5 cut(s)	987, 1297, 2062, 2101, 2350
Lsp1109I	GCAGC	9 cut(s)	751, 866, 1054, 1273, 1301, 1321, 1366, 1369, 2819
LweI	GCATC	6 cut(s)	274, 937, 1036, 1166, 1747, 2393
MabI	ACCWGGT	1 cut(s)	2193
MaeI	CTAG	9 cut(s)	159, 411, 903, 1788, 1929, 2360, 2370, 2486, 2535
MaeII	ACGT	5 cut(s)	612, 801, 1676, 1983, 2832
MalI	GATC	8 cut(s)	462, 1324, 1825, 2040, 2610, 2648, 2698, 3041
MboI	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
MfeI	CAATTG	1 cut(s)	428
MflI	RGATCY	3 cut(s)	1322, 1823, 3039
MhlI	GDGCHC	4 cut(s)	962, 1462, 2523, 3197
MlsI	TGGCCA	2 cut(s)	2978, 3281
MluNI	TGGCCA	2 cut(s)	2978, 3281
MmeI	TCCRAC	4 cut(s)	1609, 1872, 2202, 2225
Mox20I	TGGCCA	2 cut(s)	2978, 3281
MroI	TCCGGA	1 cut(s)	1298
MroXI	GAANNNNTTC	3 cut(s)	1255, 1660, 3062
MscI	TGGCCA	2 cut(s)	2978, 3281
MseI	TTAA	8 cut(s)	321, 375, 692, 1172, 1389, 1412, 1512, 1866
MslI	CAYNNNNRTG	2 cut(s)	1929, 2823
Msp20I	TGGCCA	2 cut(s)	2978, 3281
MspA1I	CMGCKG	3 cut(s)	499, 857, 2882
MspI	CCGG	5 cut(s)	299, 388, 1299, 2771, 3249
MspR9I	CCNGG	6 cut(s)	300, 389, 1698, 2060, 2195, 2481
MunI	CAATTG	1 cut(s)	428
MvaI	CCWGG	4 cut(s)	1698, 2060, 2195, 2481
MwoI	GCNNNNNNNGC	8 cut(s)	389, 534, 635, 1334, 2578, 2611, 2620, 3249
NciI	CCSGG	2 cut(s)	300, 389
NcoI	CCATGG	1 cut(s)	231
NdeI	CATATG	1 cut(s)	936
NdeII	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
NmeAIII	GCCGAG	2 cut(s)	48, 2551
NspI	RCATGY	1 cut(s)	1928
NspV	TTCGAA	1 cut(s)	2732
OliI	CACNNNNGTG	1 cut(s)	2823
PaeR7I	CTCGAG	1 cut(s)	583
PagI	TCATGA	1 cut(s)	1273
PcsI	WCGNNNNNNNCGW	1 cut(s)	99
PdmI	GAANNNNTTC	3 cut(s)	1255, 1660, 3062
PshAI	GACNNNNGTC	2 cut(s)	23, 1569
PshBI	ATTAAT	1 cut(s)	1389
Psp1406I	AACGTT	1 cut(s)	2832
Psp6I	CCWGG	4 cut(s)	1696, 2058, 2193, 2479
PspEI	GGTNACC	2 cut(s)	1771, 2189
PspGI	CCWGG	4 cut(s)	1696, 2058, 2193, 2479
PspPI	GGNCC	4 cut(s)	520, 2302, 2597, 2754
PspXI	VCTCGAGB	1 cut(s)	583
PsrI	GAACNNNNNNTAC	6 cut(s)	495, 527, 1386, 1418, 3073, 3105
PstI	CTGCAG	1 cut(s)	1291
PsuI	RGATCY	3 cut(s)	1322, 1823, 3039
PvuII	CAGCTG	3 cut(s)	499, 857, 2882
RsaI	GTAC	8 cut(s)	545, 953, 1914, 2140, 2589, 2851, 2886, 3366
RsaNI	GTAC	8 cut(s)	544, 952, 1913, 2139, 2588, 2850, 2885, 3365
RseI	CAYNNNNRTG	2 cut(s)	1929, 2823
SalI	GTCGAC	1 cut(s)	56
SaqAI	TTAA	8 cut(s)	321, 375, 692, 1172, 1389, 1412, 1512, 1866
Sau3AI	GATC	8 cut(s)	460, 1322, 1823, 2038, 2608, 2646, 2696, 3039
Sau96I	GGNCC	4 cut(s)	520, 2302, 2597, 2754
ScrFI	CCNGG	6 cut(s)	300, 389, 1698, 2060, 2195, 2481
SduI	GDGCHC	4 cut(s)	962, 1462, 2523, 3197
SexAI	ACCWGGT	1 cut(s)	2193
SfaNI	GCATC	6 cut(s)	274, 937, 1036, 1166, 1747, 2393
SfcI	CTRYAG	2 cut(s)	873, 1287
Sfr274I	CTCGAG	1 cut(s)	583
SfuI	TTCGAA	1 cut(s)	2732
SinI	GGWCC	4 cut(s)	520, 2302, 2597, 2754
SlaI	CTCGAG	1 cut(s)	583
SmiMI	CAYNNNNRTG	2 cut(s)	1929, 2823
SmlI	CTYRAG	5 cut(s)	344, 583, 1106, 2079, 2624
SmoI	CTYRAG	5 cut(s)	344, 583, 1106, 2079, 2624
SpeI	ACTAGT	1 cut(s)	1787
SsiI	CCGC	6 cut(s)	164, 888, 1081, 1477, 2690, 3207
SspI	AATATT	1 cut(s)	379
SspMI	CTAG	9 cut(s)	159, 411, 903, 1788, 1929, 2360, 2370, 2486, 2535
StyD4I	CCNGG	6 cut(s)	298, 387, 1696, 2058, 2193, 2479
StyI	CCWWGG	6 cut(s)	231, 1818, 2485, 2641, 2706, 3053
TaiI	ACGT	5 cut(s)	615, 804, 1679, 1986, 2835
TaqI	TCGA	7 cut(s)	57, 93, 174, 584, 1755, 2732, 3000
TaqII	GACCGA	2 cut(s)	2290, 2301
TatI	WGTACW	3 cut(s)	1912, 2587, 2884
TauI	GCSGC	1 cut(s)	2692
Tru1I	TTAA	8 cut(s)	321, 375, 692, 1172, 1389, 1412, 1512, 1866
Tru9I	TTAA	8 cut(s)	321, 375, 692, 1172, 1389, 1412, 1512, 1866
TscAI	CASTG	9 cut(s)	690, 967, 1044, 1460, 1509, 1603, 2050, 2827, 2986
TseI	GCWGC	9 cut(s)	739, 854, 1067, 1286, 1289, 1334, 1379, 1382, 2807
TspGWI	ACGGA	2 cut(s)	53, 2015
TspRI	CASTG	9 cut(s)	690, 967, 1044, 1460, 1509, 1603, 2050, 2827, 2986
VneI	GTGCAC	1 cut(s)	2519
VpaK11BI	GGWCC	4 cut(s)	520, 2302, 2597, 2754
VspI	ATTAAT	1 cut(s)	1389
XagI	CCTNNNNNAGG	1 cut(s)	2484
XceI	RCATGY	1 cut(s)	1928
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1036, 2477, 2648
XhoI	CTCGAG	1 cut(s)	583
XmaJI	CCTAGG	1 cut(s)	2485
XmiI	GTMKAC	2 cut(s)	57, 1402
XmnI	GAANNNNTTC	3 cut(s)	1255, 1660, 3062
XspI	CTAG	9 cut(s)	159, 411, 903, 1788, 1929, 2360, 2370, 2486, 2535
ZraI	GACGTC	2 cut(s)	613, 1677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.