Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008609

Physical Location & Seq

Genomic Coordinates Forward (+)

9731597 .. 9738531

Gene Structure 6935 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3882 bp

ATGGTCGCTGGTGGATTTGGGTGGAACAATTTCATAGAAATCGAGGAAATTTTAGGGGGTGGTGTGATGGGGAAAGACGATGAAAGTGCGAAAGTGGTTGAAGATGTAGAAGAAGGGGAAATTTCGGATTCGACTTCGGTGGAGGAGATCAGTGAGGAAGATTTTGTCAAGCAGGAGAAGCAGCAGGAAGTTAAGGTGGTGGTTCCGCTGCCGCCGCCACCCAAGGATCAGTCTAAATCCAACGGCGGAGATGATGCTAGGGTTTGGACGATGCGCGACATCTACAACTACCCGGGATTTCGGGGGTACAGGTCCGGCTTGGTTAACCTCGCTTGGGCTCAGGCCGTACAGAATAAGCCGCTCAATCAGCTTCTGCAGGTGAATGACCCCGACGAGAAATTGAAGCGATCGTCGTCGTCGCCGTCGTTGATTTCGTCCGAAAGGAGCAACGCGAAGGAAGTGGATGGAGTGGTGATTGTTGATAGTGGTGATGAAATGGATGCTGAGAAGGAGGAAGGGGAGTTGGAGGAGGGCGAGATTGATTTGGATTCGGAGCCTGCCGATGGCGAAAACACGGCGGCAGCTGAGGAGGCTAGAGATGGTGTTTTGGTTTGTGATGATATGGATGTTGATAATTCCGAGACTGGTTTGAAGAAGCGAGTGAGCTCGATTCGGGAGGCTTTGGAAAGCGTTACTGTAAATGAAGCAGAGAAATCATTTGGGGATGTTTGTCTCCAGTTACTCAACACTTTGGAGAGCTTGCGCGGAGTGCTTTCGGAAACTAATGTTTCCACAAAGGAAGCTCTTGTTCAACCGTCCTTCACTGCAGTACAAGCTATCAGCTCTGTGTTCTGCTCAATGAGACCTGACCAAAAGGAACAAAACAAAGATATTATATCAAGGGTACTTTCTTCTGTGAAGAATGATCCTCCTCTTCTTGCCCCTGAGCAGATAAAAGAGATAGGGGTCATGATATCCTCTGTGGATTCCCCTGATGTTTTCCTCCAAACTAGAGCAGCCATTACAGATAATGAGATACAGGTTATTGGTGGGGTTAATAATAAGAATTCTGAAGCTGTACATGCTGGTTCTTCAGCTAATTTTGCTTCAGACACTGTTGTATCTGGGGTTTATAGCAATCCATTTGTGTTGTCCGAAGTACCAAGACCAGGAGTATCTATTTTGAAGGGTAGGAGGGTCGTGCTTCCCCTGTTAGACACTCACAAGGATCATGATGCAGATAGCCTTCCTTCTCCAACACGAGAATCCCCATCATGTTTTCCTGTTCAGAACACATTGGTTGTTACTGATAGGATGGTAAAACCACAGCCTGATACCTCTAGGGTGACGCCAAATGCTGGAGGTTCTGGATTGCATCCTTATGATACTGATGCTCTTAAAGCTGTATCCACTTATCAACAAATTAATCGGACTTCTTTTTTCATGAGCGAAAGACTTCCAAGTCCAACCCCATCAGAAGACGGCGATAATGGGGATGATGATACTGTTGGAGAGATTTCTAGTTCTTGTGCTAGTAATTTAAGAACAGGTCCTCCTATTTCGGGACAGCAAGTTGTTTCTCCCTTCCCTATTCCTGTGGGTAGCTCCAGCATGCAAGAACGATTTACAGGTAAAAGTGCTGCCCCTGCAAGTTCTGGATCTAATATCACCATAAAAGCTCCAACTAGGAATAGAGATCCTAGGCTTCGGTTGTCCAATTCTGATATGGGTGCCTTGAATCTCAACCCACAGCCCTTGACAGTGCATAGTGCACCTAAAGTAGATTCAGTTATAACGTTAAGCTCGAGAAAGCAAAAACCCCTTGAGGATTCTAAATTTGATGGTCCTGCATTAAAGAGGCAAAGGAATACATTGGATAACTCAGGTTTTGTTAAAGATCCAAAAACTGCTTCTGGAAGTGGTGGCTGGTTGGAGGACATTGGTGGTGTTGGACCTCATTTAATTAGCAAGAATCAGACAGTGGAGAACACACAGTCTGATCCCAGACAGGTGGTTAATGATGTAAGTAGTTCTAGTACTGCTGATGGAAACTCCAATGGCCCAAACAGTTCAAATGAGCCTTTGTCGGTGATGGATTTAAGCACGGCTTCCCTGCCTGCGTTAGTTAAGGACATTGCTGTGAATTCTGCAATATTTAAAGATATTGCTGTGAATCCAACCATGCTGTTAAACATACTTAAGCTGGGACAGCAGCAAAGGTTAGCTGCAGAAGCCCAGCAGAAGGCTGCTGATCCTGAAAAAAGCATGACAAATCCTATAAGCTCAAGTTCAATACTAAGATCAAATGCATCGGTGAGTGTTCCTTCAAAGACCACAGCCATGTTGCAGACACTAGCAGGAACACTTCCCGTTAGTTCACAAAAAGCTCCGACGGATGAGTCAGGGAAAGTCCGCATGAAACCCCGTGACCCTCGCCGTGTTCTCCATGCTAATGCACTTCAAAAGAATGGGAGTCTGGGACAAGAGCAGTTCAGAAATATTGTAACCCCGCTGTCAAGCAGCCAGGGGAATAAGGATAATCTTAATGGCCAAGAGCACGATGGTCAGGCGGATATGAAGCTAGTCACTTCTCAATCAGTAGAGGCCCCTGACATTGCTCGCCAATTCACAAAGAATCTGAAAAATATTGCTGATATCATGTCTGTTTCTAATGGATCAACGAGTCCAGCAATCGCTTCTCAGAGTGTTTCTTCCCAACCAGTTCCAATCAAGAATGAGAGAATAGATCCAAAGACTGAGGAGCAGGGTACTGGAAGTATTTCTGCTTCTGAAGCAGCTGCAGCATGTCCCTCTCCTTCTGCCCCCATGTGGGGAGATGTTGAACATCTGTTTGAAGGATATGATGACCAGCAAAAAGCCGCTATCCAGAGAGAGAGAGCAAGGAGGATAGAAGAACAGAAGAAAATGTTTGCAGCTCGCAAGCTTTGCCTCGTCTTGGATCTAGATCACACACTTCTTAATTCAGCTAAGTTTATTGAAGTAGACCCAGTGCATGATGAGATTTTGAGAAAGAAAGAGGAACAGGATCGTGAAAAACCACAGCGACATCTCTTTCGATTCCATCACATGGGAATGTGGACCAAATTACGTCCTGGGGTTTGGAATTTTTTGGAGAGGGCTAGTCAACTTTTTGAGTTGCATCTTTACACAATGGGGAACAAGCTATATGCAACGGAAATGGCAAAGGTGCTAGATCCAACAGGGGTACTTTTTGCTGGACGAGTTATTTCTAGAGGCGACGATGGAGATCCTGATGATGGTGATGCTCCGAAGAGTAAGGATCTGGAAGGAGTTTTGGGTATGGAATCAGCTGTTGTGATTATAGATGATTCTGTGAGGGTCTGGCCGCATAACAAAATGAACTTGATAGTTGTGGAACGGTATACGTACTTTCCTTGTAGCAGACGACAATTTGGACTTTTAGGTCCCTCTCTGCTCGAGATTGACCATGATGAGAGACAAGAAGATGGGACTTTGGCATCCTCATTGTCGGTTATTGAGAAGATGCATCAGATCTTTTTCTCCCATCCCTCCCTAGATGAAGCAGATGTTAGAAATATTCTAGCCTCTGAGCAGCGGAAGATTTTGAACGGTTGTCGTATAGTATTTAGCAGGGTATTTCCAGTTGGTGAGGTCAATCCTCACCTACATCCGCTGTGGCAGACAGCTGAACAGTTTGGTGCTGTGTGCACCAACTATATAGATGACCAGGTCACCCATGTAGTTGCCAATTCCCTCGGGACTGACAAGGTGAATTGGGCTATTTCTTCGGGGAAATTTGTTGTCCATCCTGGCTGGGTGGAAGCATCAGCTTTGCTTTACAGGAGGGCAAATGAGCAGGATTTTGCCATTAAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008022 GO:0008150 GO:0008152 GO:0008420 GO:0009628 GO:0009651 GO:0009787 GO:0009788 GO:0009889 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1902494 GO:1903506 GO:1905957 GO:1905958 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT NIF PTCB-BRCT

Protein Analysis

1294

Amino Acids

140.67

Weight (kDa)

5.03

Isoelectric Point (pI)

50.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_CPL3	PF25505	221 - 339	2.7e-46	CPL3 ARM repeat domain
NIF	PF03031	982 - 1136	1.4e-22	NLI interacting factor-like phosphatase
BRCT	PF00533	1200 - 1277	2e-08	BRCA1 C Terminus (BRCT) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008609)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33540 AT2G33540
fragaria_vesca	FvH4_4g22870 FvH4_4g22870 FvH4_4g22870
malus_domestica	MD13G1129000.v1.1 MD16G1128700.v1.1 MD16G1128800.v1.1
prunus_persica	Prupe.1G217600_v2.0.a1
pyrus_communis	pycom13g11190 pycom13g11200 pycom16g10920
rosa_chinensis	RchiOBHm_Chr4g0429111 RchiOBHm_Chr4g0429121
rosa_laevigata	RLG00000007096
rosa_multiflora	Rmu_sc0000846.1_g000014
rosa_roxburghii	Rroxscaffold_5G00370740
rosa_rugosa	Rorug04G0233100
rosa_samantha	Rh4AG289100 Rh4BG295100 Rh4CG310500 Rh4DG292000
rosa_wichuraiana	Rw4G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1793
AarI	CACCTGC	1 cut(s)	367
AasI	GACNNNNNNGTC	3 cut(s)	1461, 2398, 3444
Acc36I	ACCTGC	1 cut(s)	367
AccB1I	GGYRCC	1 cut(s)	1730
AccB7I	CCANNNNNTGG	2 cut(s)	1358, 3088
AccBSI	CCGCTC	1 cut(s)	361
AccI	GTMKAC	2 cut(s)	3003, 3404
AccII	CGCG	3 cut(s)	276, 452, 765
AclI	AACGTT	1 cut(s)	1796
AcoI	YGGCCR	2 cut(s)	2548, 3365
AcsI	RAATTY	7 cut(s)	48, 120, 1066, 1835, 2143, 3124, 3797
AcuI	CTGAAG	4 cut(s)	1077, 1092, 1092, 2811
AcyI	GRCGYC	1 cut(s)	1349
AflII	CTTAAG	1 cut(s)	2198
AjnI	CCWGG	5 cut(s)	1168, 2523, 3112, 3729, 3811
AjuI	GAANNNNNNNTTGG	2 cut(s)	1894, 1926
AloI	GAACNNNNNNTCC	2 cut(s)	1537, 1569
Alw21I	GWGCWC	4 cut(s)	668, 1774, 2559, 3713
Alw26I	GTCTC	4 cut(s)	635, 737, 856, 3472
Alw44I	GTGCAC	2 cut(s)	1770, 3709
AlwNI	CAGNNNCTG	2 cut(s)	373, 1115
Ama87I	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
AoxI	GGCC	5 cut(s)	342, 2059, 2548, 2604, 3365
ApaLI	GTGCAC	2 cut(s)	1770, 3709
ApoI	RAATTY	7 cut(s)	48, 120, 1066, 1835, 2143, 3124, 3797
ArsI	GACNNNNNNTTYG	2 cut(s)	3057, 3089
AseI	ATTAAT	1 cut(s)	1425
Asp700I	GAANNNNTTC	3 cut(s)	29, 1455, 2779
AspA2I	CCTAGG	1 cut(s)	1700
AspLEI	GCGC	2 cut(s)	276, 765
AspS9I	GGNCC	8 cut(s)	312, 1550, 1844, 1952, 2060, 2605, 3099, 3446
AsuC2I	CCSGG	2 cut(s)	293, 294
AvaI	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
AvaII	GGWCC	6 cut(s)	312, 1550, 1844, 1952, 3099, 3446
AvrII	CCTAGG	1 cut(s)	1700
BaeGI	GKGCMC	2 cut(s)	1774, 3713
BaeI	ACNNNNGTAYC	2 cut(s)	1104, 1137
BalI	TGGCCA	1 cut(s)	2550
BanI	GGYRCC	1 cut(s)	1730
BanII	GRGCYC	2 cut(s)	340, 668
BarI	GAAGNNNNNNTAC	2 cut(s)	2770, 2802
BauI	CACGAG	1 cut(s)	1260
BbsI	GAAGAC	1 cut(s)	1485
Bbv12I	GWGCWC	4 cut(s)	668, 1774, 2559, 3713
BbvCI	CCTCAGC	1 cut(s)	585
BceAI	ACGGC	7 cut(s)	259, 329, 406, 591, 1498, 2121, 2421
BcgI	CGANNNNNNTGC	2 cut(s)	68, 102
BciT130I	CCWGG	5 cut(s)	1170, 2525, 3114, 3731, 3813
BciVI	GTATCC	1 cut(s)	1417
BcnI	CCSGG	2 cut(s)	293, 294
BcoDI	GTCTC	4 cut(s)	635, 737, 856, 3472
BfmI	CTRYAG	4 cut(s)	374, 825, 2226, 2799
BfrI	CTTAAG	1 cut(s)	2198
BfuAI	ACCTGC	1 cut(s)	367
BfuI	GTATCC	1 cut(s)	1417
BglII	AGATCT	1 cut(s)	3534
BlnI	CCTAGG	1 cut(s)	1700
BmcAI	AGTACT	1 cut(s)	2038
Bme1390I	CCNGG	7 cut(s)	293, 294, 1170, 2525, 3114, 3731, 3813
Bme18I	GGWCC	6 cut(s)	312, 1550, 1844, 1952, 3099, 3446
BmeT110I	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
BmgT120I	GGNCC	8 cut(s)	312, 1550, 1844, 1952, 2060, 2605, 3099, 3446
BmiI	GGNNCC	5 cut(s)	204, 555, 1732, 2607, 3448
BmrFI	CCNGG	7 cut(s)	293, 294, 1170, 2525, 3114, 3731, 3813
BmrI	ACTGGG	1 cut(s)	3002
BmuI	ACTGGG	1 cut(s)	3002
BpiI	GAAGAC	1 cut(s)	1485
BpmI	CTGGAG	3 cut(s)	719, 1380, 1591
Bpu10I	CCTNAGC	3 cut(s)	339, 585, 945
BpuEI	CTTGAG	2 cut(s)	1844, 2269
BpuMI	CCSGG	2 cut(s)	293, 294
BsaAI	YACGTR	1 cut(s)	3408
BsaBI	GATNNNNATC	2 cut(s)	2964, 3267
BsaHI	GRCGYC	1 cut(s)	1349
BsaI	GGTCTC	1 cut(s)	856
BsaJI	CCNNGG	6 cut(s)	222, 292, 1700, 2524, 3113, 3757
BsaXI	ACNNNNNCTCC	2 cut(s)	1537, 1567
Bse1I	ACTGG	6 cut(s)	649, 736, 2720, 2776, 3008, 3644
Bse3DI	GCAATG	2 cut(s)	2133, 2613
Bse8I	GATNNNNATC	2 cut(s)	2964, 3267
BseBI	CCWGG	5 cut(s)	1170, 2525, 3114, 3731, 3813
BseDI	CCNNGG	6 cut(s)	222, 292, 1700, 2524, 3113, 3757
BseJI	GATNNNNATC	2 cut(s)	2964, 3267
BseMI	GCAATG	2 cut(s)	2133, 2613
BseMII	CTCAG	8 cut(s)	353, 495, 576, 936, 1896, 2714, 2748, 3582
BseNI	ACTGG	6 cut(s)	649, 736, 2720, 2776, 3008, 3644
BseRI	GAGGAG	5 cut(s)	158, 542, 602, 921, 2774
BseSI	GKGCMC	2 cut(s)	1774, 3713
BseYI	CCCAGC	3 cut(s)	2203, 2235, 3816
Bsh1236I	CGCG	3 cut(s)	276, 452, 765
Bsh1285I	CGRYCG	1 cut(s)	410
BshFI	GGCC	5 cut(s)	344, 2061, 2550, 2606, 3367
BshNI	GGYRCC	1 cut(s)	1730
BsiEI	CGRYCG	1 cut(s)	410
BsiHKAI	GWGCWC	4 cut(s)	668, 1774, 2559, 3713
BsiHKCI	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
BsiSI	CCGG	2 cut(s)	293, 315
BslFI	GGGAC	7 cut(s)	1578, 2220, 2493, 2793, 3432, 3505, 3775
BsmAI	GTCTC	4 cut(s)	635, 737, 856, 3472
BsmFI	GGGAC	7 cut(s)	1578, 2220, 2493, 2793, 3432, 3505, 3775
BsnI	GGCC	5 cut(s)	344, 2061, 2550, 2606, 3367
Bso31I	GGTCTC	1 cut(s)	856
BsoBI	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
Bsp1286I	GDGCHC	5 cut(s)	340, 668, 1774, 2559, 3713
Bsp1407I	TGTACA	1 cut(s)	1078
BspANI	GGCC	5 cut(s)	344, 2061, 2550, 2606, 3367
BspCNI	CTCAG	8 cut(s)	352, 496, 577, 937, 1895, 2713, 2749, 3583
BspFNI	CGCG	3 cut(s)	276, 452, 765
BspHI	TCATGA	3 cut(s)	969, 1231, 1443
BspLI	GGNNCC	5 cut(s)	204, 555, 1732, 2607, 3448
BspMAI	CTGCAG	4 cut(s)	378, 829, 2230, 2803
BspMI	ACCTGC	1 cut(s)	367
BspT107I	GGYRCC	1 cut(s)	1730
BspTI	CTTAAG	1 cut(s)	2198
BspTNI	GGTCTC	1 cut(s)	856
BsrBI	CCGCTC	1 cut(s)	361
BsrDI	GCAATG	2 cut(s)	2133, 2613
BsrGI	TGTACA	1 cut(s)	1078
BsrI	ACTGG	6 cut(s)	649, 736, 2720, 2776, 3008, 3644
BssECI	CCNNGG	6 cut(s)	222, 292, 1700, 2524, 3113, 3757
BssNAI	GTATAC	1 cut(s)	3405
BssNI	GRCGYC	1 cut(s)	1349
BssSI	CACGAG	1 cut(s)	1260
BssT1I	CCWWGG	2 cut(s)	222, 1700
Bst1107I	GTATAC	1 cut(s)	3405
Bst2BI	CACGAG	1 cut(s)	1260
Bst2UI	CCWGG	5 cut(s)	1170, 2525, 3114, 3731, 3813
Bst6I	CTCTTC	2 cut(s)	939, 3287
BstACI	GRCGYC	1 cut(s)	1349
BstAFI	CTTAAG	1 cut(s)	2198
BstAPI	GCANNNNNTGC	1 cut(s)	2946
BstAUI	TGTACA	1 cut(s)	1078
BstBAI	YACGTR	1 cut(s)	3408
BstC8I	GCNNGC	7 cut(s)	558, 761, 1613, 2118, 2620, 2938, 2942
BstEII	GGTNACC	1 cut(s)	3733
BstFNI	CGCG	3 cut(s)	276, 452, 765
BstHHI	GCGC	2 cut(s)	276, 765
BstMAI	GTCTC	4 cut(s)	635, 737, 856, 3472
BstMCI	CGRYCG	1 cut(s)	410
BstNI	CCWGG	5 cut(s)	1170, 2525, 3114, 3731, 3813
BstNSI	RCATGY	3 cut(s)	1085, 1615, 2808
BstPI	GGTNACC	1 cut(s)	3733
BstSCI	CCNGG	7 cut(s)	291, 292, 1168, 2523, 3112, 3729, 3811
BstSFI	CTRYAG	4 cut(s)	374, 825, 2226, 2799
BstSLI	GKGCMC	2 cut(s)	1774, 3713
BstSNI	TACGTA	1 cut(s)	3408
BstUI	CGCG	3 cut(s)	276, 452, 765
BstV2I	GAAGAC	1 cut(s)	1485
BstX2I	RGATCY	9 cut(s)	1658, 1696, 1897, 2746, 2959, 3214, 3268, 3301, 3534
BstXI	CCANNNNNNTGG	1 cut(s)	2011
BstYI	RGATCY	9 cut(s)	1658, 1696, 1897, 2746, 2959, 3214, 3268, 3301, 3534
BstZ17I	GTATAC	1 cut(s)	3405
BsuI	GTATCC	1 cut(s)	1417
BsuRI	GGCC	5 cut(s)	344, 2061, 2550, 2606, 3367
BtsI	GCAGTG	1 cut(s)	822
BtsIMutI	CAGTG	6 cut(s)	157, 822, 1113, 1767, 1986, 3015
BveI	ACCTGC	1 cut(s)	367
Cac8I	GCNNGC	7 cut(s)	558, 761, 1613, 2118, 2620, 2938, 2942
CaiI	CAGNNNCTG	2 cut(s)	373, 1115
CciI	TCATGA	3 cut(s)	969, 1231, 1443
CfoI	GCGC	2 cut(s)	276, 765
Cfr13I	GGNCC	8 cut(s)	312, 1550, 1844, 1952, 2060, 2605, 3099, 3446
Cfr9I	CCCGGG	1 cut(s)	292
CseI	GACGC	1 cut(s)	1357
CsiI	ACCWGGT	1 cut(s)	3729
CspCI	CAANNNNNGTGG	2 cut(s)	1737, 1772
DraI	TTTAAA	1 cut(s)	2158
DrdI	GACNNNNNNGTC	3 cut(s)	1461, 2398, 3444
DseDI	GACNNNNNNGTC	3 cut(s)	1461, 2398, 3444
EaeI	YGGCCR	2 cut(s)	2548, 3365
Eam1104I	CTCTTC	2 cut(s)	939, 3287
EarI	CTCTTC	2 cut(s)	939, 3287
EciI	GGCGGA	2 cut(s)	261, 2585
Ecl136II	GAGCTC	1 cut(s)	666
Eco105I	TACGTA	1 cut(s)	3408
Eco130I	CCWWGG	2 cut(s)	222, 1700
Eco24I	GRGCYC	2 cut(s)	340, 668
Eco31I	GGTCTC	1 cut(s)	856
Eco32I	GATATC	2 cut(s)	975, 2656
Eco47I	GGWCC	6 cut(s)	312, 1550, 1844, 1952, 3099, 3446
Eco53kI	GAGCTC	1 cut(s)	666
Eco57I	CTGAAG	4 cut(s)	1077, 1092, 1092, 2811
Eco88I	CYCGRG	4 cut(s)	292, 1804, 3458, 3758
Eco91I	GGTNACC	1 cut(s)	3733
EcoICRI	GAGCTC	1 cut(s)	666
EcoO109I	RGGNCCY	3 cut(s)	1550, 2605, 3446
EcoO65I	GGTNACC	1 cut(s)	3733
EcoRI	GAATTC	2 cut(s)	1066, 2143
EcoRII	CCWGG	5 cut(s)	1168, 2523, 3112, 3729, 3811
EcoRV	GATATC	2 cut(s)	975, 2656
EcoT14I	CCWWGG	2 cut(s)	222, 1700
EcoT22I	ATGCAT	2 cut(s)	2311, 3531
EcoT38I	GRGCYC	2 cut(s)	340, 668
ErhI	CCWWGG	2 cut(s)	222, 1700
FalI	AAGNNNNNCTT	2 cut(s)	2092, 2124
FaqI	GGGAC	7 cut(s)	1578, 2220, 2493, 2793, 3432, 3505, 3775
FauI	CCCGC	1 cut(s)	2517
FblI	GTMKAC	2 cut(s)	3003, 3404
FriOI	GRGCYC	2 cut(s)	340, 668
GlaI	GCGC	2 cut(s)	275, 764
GsaI	CCCAGC	3 cut(s)	2207, 2239, 3820
GsuI	CTGGAG	3 cut(s)	719, 1380, 1591
HaeIII	GGCC	5 cut(s)	344, 2061, 2550, 2606, 3367
HapII	CCGG	2 cut(s)	293, 315
HgaI	GACGC	1 cut(s)	1357
HhaI	GCGC	2 cut(s)	276, 765
Hin1I	GRCGYC	1 cut(s)	1349
Hin6I	GCGC	2 cut(s)	274, 763
HinP1I	GCGC	2 cut(s)	274, 763
HincII	GTYRAC	2 cut(s)	325, 3146
HindII	GTYRAC	2 cut(s)	325, 3146
HindIII	AAGCTT	1 cut(s)	2942
HpaI	GTTAAC	1 cut(s)	325
HpaII	CCGG	2 cut(s)	293, 315
Hpy166II	GTNNAC	8 cut(s)	325, 1772, 2378, 3004, 3099, 3146, 3405, 3711
Hpy8I	GTNNAC	8 cut(s)	325, 1772, 2378, 3004, 3099, 3146, 3405, 3711
Hpy99I	CGWCG	7 cut(s)	395, 415, 418, 421, 427, 2395, 3263
HpyCH4IV	ACGT	3 cut(s)	1796, 3109, 3407
HpySE526I	ACGT	3 cut(s)	1796, 3109, 3407
Hsp92I	GRCGYC	1 cut(s)	1349
HspAI	GCGC	2 cut(s)	274, 763
KspAI	GTTAAC	1 cut(s)	325
LmnI	GCTCC	7 cut(s)	444, 553, 1610, 1684, 2392, 2761, 3292
MabI	ACCWGGT	1 cut(s)	3729
MaeII	ACGT	3 cut(s)	1796, 3109, 3407
MaeIII	GTNAC	8 cut(s)	691, 738, 1303, 1345, 2426, 2503, 2584, 3733
MbiI	CCGCTC	1 cut(s)	361
MflI	RGATCY	9 cut(s)	1658, 1696, 1897, 2746, 2959, 3214, 3268, 3301, 3534
MhlI	GDGCHC	5 cut(s)	340, 668, 1774, 2559, 3713
MlsI	TGGCCA	1 cut(s)	2550
MluNI	TGGCCA	1 cut(s)	2550
MlyI	GAGTC	3 cut(s)	2408, 2482, 2692
Mox20I	TGGCCA	1 cut(s)	2550
Mph1103I	ATGCAT	2 cut(s)	2311, 3531
MroXI	GAANNNNTTC	3 cut(s)	29, 1455, 2779
MscI	TGGCCA	1 cut(s)	2550
MslI	CAYNNNNRTG	5 cut(s)	1380, 2138, 2339, 2451, 3092
Msp20I	TGGCCA	1 cut(s)	2550
MspA1I	CMGCKG	8 cut(s)	208, 584, 2512, 2798, 3332, 3598, 3676, 3689
MspCI	CTTAAG	1 cut(s)	2198
MspI	CCGG	2 cut(s)	293, 315
MspR9I	CCNGG	7 cut(s)	293, 294, 1170, 2525, 3114, 3731, 3813
MvaI	CCWGG	5 cut(s)	1170, 2525, 3114, 3731, 3813
MvnI	CGCG	3 cut(s)	276, 452, 765
NciI	CCSGG	2 cut(s)	293, 294
NlaIV	GGNNCC	5 cut(s)	204, 555, 1732, 2607, 3448
NmuCI	GTSAC	4 cut(s)	1345, 2426, 2584, 3733
NsiI	ATGCAT	2 cut(s)	2311, 3531
NspI	RCATGY	3 cut(s)	1085, 1615, 2808
PaeI	GCATGC	1 cut(s)	1615
PaeR7I	CTCGAG	2 cut(s)	1804, 3458
PagI	TCATGA	3 cut(s)	969, 1231, 1443
PaqCI	CACCTGC	1 cut(s)	367
PcsI	WCGNNNNNNNCGW	4 cut(s)	419, 422, 431, 1802
PdmI	GAANNNNTTC	3 cut(s)	29, 1455, 2779
PflFI	GACNNNGTC	1 cut(s)	3731
PflMI	CCANNNNNTGG	2 cut(s)	1358, 3088
Ple19I	CGATCG	1 cut(s)	410
PleI	GAGTC	3 cut(s)	2407, 2481, 2691
PpsI	GAGTC	3 cut(s)	2407, 2481, 2691
Ppu21I	YACGTR	1 cut(s)	3408
PpuMI	RGGWCCY	2 cut(s)	1550, 3446
PshBI	ATTAAT	1 cut(s)	1425
PsiI	TTATAA	1 cut(s)	1793
Psp124BI	GAGCTC	1 cut(s)	668
Psp1406I	AACGTT	1 cut(s)	1796
Psp5II	RGGWCCY	2 cut(s)	1550, 3446
Psp6I	CCWGG	5 cut(s)	1168, 2523, 3112, 3729, 3811
PspEI	GGTNACC	1 cut(s)	3733
PspFI	CCCAGC	3 cut(s)	2203, 2235, 3816
PspGI	CCWGG	5 cut(s)	1168, 2523, 3112, 3729, 3811
PspN4I	GGNNCC	5 cut(s)	204, 555, 1732, 2607, 3448
PspPI	GGNCC	8 cut(s)	312, 1550, 1844, 1952, 2060, 2605, 3099, 3446
PspPPI	RGGWCCY	2 cut(s)	1550, 3446
PstI	CTGCAG	4 cut(s)	378, 829, 2230, 2803
PstNI	CAGNNNCTG	2 cut(s)	373, 1115
PsuI	RGATCY	9 cut(s)	1658, 1696, 1897, 2746, 2959, 3214, 3268, 3301, 3534
PsyI	GACNNNGTC	1 cut(s)	3731
PvuI	CGATCG	1 cut(s)	410
PvuII	CAGCTG	4 cut(s)	584, 2798, 3332, 3689
RseI	CAYNNNNRTG	5 cut(s)	1380, 2138, 2339, 2451, 3092
SacI	GAGCTC	1 cut(s)	668
Sau96I	GGNCC	8 cut(s)	312, 1550, 1844, 1952, 2060, 2605, 3099, 3446
ScaI	AGTACT	1 cut(s)	2038
SchI	GAGTC	3 cut(s)	2408, 2482, 2692
ScrFI	CCNGG	7 cut(s)	293, 294, 1170, 2525, 3114, 3731, 3813
SduI	GDGCHC	5 cut(s)	340, 668, 1774, 2559, 3713
SexAI	ACCWGGT	1 cut(s)	3729
SfcI	CTRYAG	4 cut(s)	374, 825, 2226, 2799
Sfr274I	CTCGAG	2 cut(s)	1804, 3458
SinI	GGWCC	6 cut(s)	312, 1550, 1844, 1952, 3099, 3446
SlaI	CTCGAG	2 cut(s)	1804, 3458
SmaI	CCCGGG	1 cut(s)	294
SmiMI	CAYNNNNRTG	5 cut(s)	1380, 2138, 2339, 2451, 3092
SmlI	CTYRAG	5 cut(s)	1804, 1823, 2198, 2284, 3458
SmoI	CTYRAG	5 cut(s)	1804, 1823, 2198, 2284, 3458
SnaBI	TACGTA	1 cut(s)	3408
SphI	GCATGC	1 cut(s)	1615
SspI	AATATT	4 cut(s)	2154, 2500, 2647, 3580
SstI	GAGCTC	1 cut(s)	668
StyD4I	CCNGG	7 cut(s)	291, 292, 1168, 2523, 3112, 3729, 3811
StyI	CCWWGG	2 cut(s)	222, 1700
TaiI	ACGT	3 cut(s)	1799, 3112, 3410
TaqI	TCGA	6 cut(s)	42, 131, 668, 1805, 3076, 3459
TatI	WGTACW	3 cut(s)	829, 1078, 2036
TauI	GCSGC	6 cut(s)	214, 217, 361, 581, 2882, 3370
TscAI	CASTG	6 cut(s)	157, 829, 1120, 1767, 1986, 3015
TseFI	GTSAC	4 cut(s)	1345, 2426, 2584, 3733
Tsp45I	GTSAC	4 cut(s)	1345, 2426, 2584, 3733
TspDTI	ATGAA	9 cut(s)	22, 96, 507, 717, 1432, 2432, 2591, 3395, 3576
TspGWI	ACGGA	2 cut(s)	2408, 3209
TspMI	CCCGGG	1 cut(s)	292
TspRI	CASTG	6 cut(s)	157, 829, 1120, 1767, 1986, 3015
Tth111I	GACNNNGTC	1 cut(s)	3731
Van91I	CCANNNNNTGG	2 cut(s)	1358, 3088
Vha464I	CTTAAG	1 cut(s)	2198
VneI	GTGCAC	2 cut(s)	1770, 3709
VpaK11BI	GGWCC	6 cut(s)	312, 1550, 1844, 1952, 3099, 3446
VspI	ATTAAT	1 cut(s)	1425
XapI	RAATTY	7 cut(s)	48, 120, 1066, 1835, 2143, 3124, 3797
XbaI	TCTAGA	2 cut(s)	2962, 3251
XceI	RCATGY	3 cut(s)	1085, 1615, 2808
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1723, 2558
XhoI	CTCGAG	2 cut(s)	1804, 3458
XmaI	CCCGGG	1 cut(s)	292
XmaJI	CCTAGG	1 cut(s)	1700
XmiI	GTMKAC	2 cut(s)	3003, 3404
XmnI	GAANNNNTTC	3 cut(s)	29, 1455, 2779
ZrmI	AGTACT	1 cut(s)	2038
Zsp2I	ATGCAT	2 cut(s)	2311, 3531

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.