Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008609

Physical Location & Seq

Genomic Coordinates Forward (+)

9424447 .. 9426899

Gene Structure 2453 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1128800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 711 bp

ATGGGGAACAAGCTATATGCTACGGAAATGGCAAAAGTGCTAGATCCAACAGGGGTACTTTTTGCTGGACGAGTCATTTCTAGAGGTGACGATGGAGATTCTGATGATGGTGATGCTCCCAAGAGTAAGGATCTGGAAGGAGTTTTGGGTATGGAATCAGCTGTTGTGATTATAGATGATTCTGTGAGGGTCTGGCCGCATAACAAACTGAACTTGATAGTTGTGGAACGTTATACGTACTTTCCTTGTAGCAGACGACAATTTGGGCTTCTAGGTCCTTCTCTACTCGAGATTGATCATGACGAGAGACAAGAAGATGGGACTTTGGCATCCTCATTGTCGGTTATTGAGAAGATACATCAGATCTTTTTCTCCCATCCCTCCCTAGATGAAGCAGATGTTAGAAATATTCTAGCCTCTGAGCAGCGGAAGATTTTGAACGGTTGTCGTATAGTATTTAGCAGGGTGTTTCCAGTTGGTGAGGTCAATCCTCACCTACATCCGCTGTGGCAGACAGCTGAACAGTTTGGTGCTGTGTGTACCAACCATATAGATGACCAGGTCACCCATGTAGTTGCCAATTCCCTTGGAACTGACAAGGTGAATTGGGCTATTTCTTTGGGAAAATTTGTTGTCCATCCTGGCTGGGTGGAAGCATCAGCTTTGCTTTATCGGAGGGCAAATGAGCAGGATTTTGCCATTAAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008022 GO:0008150 GO:0008152 GO:0008420 GO:0009628 GO:0009651 GO:0009787 GO:0009788 GO:0009889 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1902494 GO:1903506 GO:1905957 GO:1905958 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT NIF PTCB-BRCT

Protein Analysis

237

Amino Acids

26.21

Weight (kDa)

5.32

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	2 - 79	1.2e-07	NLI interacting factor-like phosphatase
BRCT	PF00533	142 - 220	1.1e-08	BRCA1 C Terminus (BRCT) domain
PTCB-BRCT	PF12738	166 - 216	4.9e-06	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008609)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33540 AT2G33540
fragaria_vesca	FvH4_4g22870 FvH4_4g22870 FvH4_4g22870
malus_domestica	MD13G1129000.v1.1 MD16G1128700.v1.1 MD16G1128800.v1.1
prunus_persica	Prupe.1G217600_v2.0.a1
pyrus_communis	pycom13g11190 pycom13g11200 pycom16g10920
rosa_chinensis	RchiOBHm_Chr4g0429111 RchiOBHm_Chr4g0429121
rosa_laevigata	RLG00000007096
rosa_multiflora	Rmu_sc0000846.1_g000014
rosa_roxburghii	Rroxscaffold_5G00370740
rosa_rugosa	Rorug04G0233100
rosa_samantha	Rh4AG289100 Rh4BG295100 Rh4CG310500 Rh4DG292000
rosa_wichuraiana	Rw4G025040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	197, 427, 503
AclI	AACGTT	1 cut(s)	229
AclWI	GGATC	2 cut(s)	38, 138
AcoI	YGGCCR	1 cut(s)	194
AcsI	RAATTY	1 cut(s)	626
AfaI	GTAC	3 cut(s)	57, 239, 541
AgsI	TTSAA	1 cut(s)	439
AjnI	CCWGG	2 cut(s)	558, 640
AluBI	AGCT	4 cut(s)	13, 161, 518, 662
AluI	AGCT	4 cut(s)	13, 161, 518, 662
Alw26I	GTCTC	1 cut(s)	301
AlwI	GGATC	2 cut(s)	38, 138
Ama87I	CYCGRG	1 cut(s)	287
AoxI	GGCC	1 cut(s)	194
ApeKI	GCWGC	1 cut(s)	424
ApoI	RAATTY	1 cut(s)	626
AspS9I	GGNCC	1 cut(s)	275
AsuHPI	GGTGA	6 cut(s)	98, 122, 485, 491, 556, 613
AvaI	CYCGRG	1 cut(s)	287
AvaII	GGWCC	1 cut(s)	275
BbvI	GCAGC	1 cut(s)	436
BccI	CCATC	5 cut(s)	86, 101, 311, 384, 645
BciT130I	CCWGG	2 cut(s)	560, 642
BclI	TGATCA	1 cut(s)	295
BcoDI	GTCTC	1 cut(s)	301
BfaI	CTAG	5 cut(s)	41, 81, 272, 386, 413
BglII	AGATCT	1 cut(s)	363
BisI	GCNGC	2 cut(s)	197, 425
BlsI	GCNGC	2 cut(s)	198, 426
Bme1390I	CCNGG	2 cut(s)	560, 642
Bme18I	GGWCC	1 cut(s)	275
BmeT110I	CYCGRG	1 cut(s)	287
BmgT120I	GGNCC	1 cut(s)	275
BmrFI	CCNGG	2 cut(s)	560, 642
BmsI	GCATC	3 cut(s)	103, 338, 665
BsaAI	YACGTR	1 cut(s)	237
BsaJI	CCNNGG	1 cut(s)	586
Bse1I	ACTGG	1 cut(s)	473
BseBI	CCWGG	2 cut(s)	560, 642
BseDI	CCNNGG	1 cut(s)	586
BseGI	GGATG	4 cut(s)	329, 376, 499, 637
BseMII	CTCAG	1 cut(s)	411
BseNI	ACTGG	1 cut(s)	473
BseXI	GCAGC	1 cut(s)	436
BseYI	CCCAGC	1 cut(s)	645
BshFI	GGCC	1 cut(s)	196
BsiHKCI	CYCGRG	1 cut(s)	287
BslFI	GGGAC	1 cut(s)	334
BsmAI	GTCTC	1 cut(s)	301
BsmFI	GGGAC	1 cut(s)	334
BsnI	GGCC	1 cut(s)	196
BsoBI	CYCGRG	1 cut(s)	287
Bsp143I	GATC	4 cut(s)	43, 130, 295, 363
BspACI	CCGC	3 cut(s)	197, 427, 503
BspANI	GGCC	1 cut(s)	196
BspCNI	CTCAG	1 cut(s)	412
BspHI	TCATGA	1 cut(s)	298
BspPI	GGATC	2 cut(s)	38, 138
BsrI	ACTGG	1 cut(s)	473
BssECI	CCNNGG	1 cut(s)	586
BssMI	GATC	4 cut(s)	43, 130, 295, 363
BssT1I	CCWWGG	1 cut(s)	586
Bst2UI	CCWGG	2 cut(s)	560, 642
Bst4CI	ACNGT	2 cut(s)	443, 525
BstBAI	YACGTR	1 cut(s)	237
BstDEI	CTNAG	1 cut(s)	420
BstEII	GGTNACC	1 cut(s)	562
BstF5I	GGATG	4 cut(s)	329, 376, 499, 637
BstKTI	GATC	4 cut(s)	46, 133, 298, 366
BstMAI	GTCTC	1 cut(s)	301
BstMBI	GATC	4 cut(s)	43, 130, 295, 363
BstNI	CCWGG	2 cut(s)	560, 642
BstPI	GGTNACC	1 cut(s)	562
BstSCI	CCNGG	2 cut(s)	558, 640
BstSNI	TACGTA	1 cut(s)	237
BstV1I	GCAGC	1 cut(s)	436
BstX2I	RGATCY	3 cut(s)	43, 130, 363
BstYI	RGATCY	3 cut(s)	43, 130, 363
BsuRI	GGCC	1 cut(s)	196
BtsCI	GGATG	4 cut(s)	329, 376, 499, 637
CciI	TCATGA	1 cut(s)	298
Cfr13I	GGNCC	1 cut(s)	275
CsiI	ACCWGGT	1 cut(s)	558
Csp6I	GTAC	3 cut(s)	56, 238, 540
CviAII	CATG	2 cut(s)	299, 569
CviJI	RGCY	9 cut(s)	13, 161, 196, 268, 416, 518, 611, 645, 662
CviKI_1	RGCY	9 cut(s)	13, 161, 196, 268, 416, 518, 611, 645, 662
CviQI	GTAC	3 cut(s)	56, 238, 540
DdeI	CTNAG	1 cut(s)	420
DpnI	GATC	4 cut(s)	45, 132, 297, 365
DpnII	GATC	4 cut(s)	43, 130, 295, 363
EaeI	YGGCCR	1 cut(s)	194
Eco105I	TACGTA	1 cut(s)	237
Eco130I	CCWWGG	1 cut(s)	586
Eco47I	GGWCC	1 cut(s)	275
Eco88I	CYCGRG	1 cut(s)	287
Eco91I	GGTNACC	1 cut(s)	562
EcoO109I	RGGNCCY	1 cut(s)	275
EcoO65I	GGTNACC	1 cut(s)	562
EcoRII	CCWGG	2 cut(s)	558, 640
EcoT14I	CCWWGG	1 cut(s)	586
ErhI	CCWWGG	1 cut(s)	586
FaeI	CATG	2 cut(s)	302, 572
FaqI	GGGAC	1 cut(s)	334
FatI	CATG	2 cut(s)	298, 568
FbaI	TGATCA	1 cut(s)	295
Fnu4HI	GCNGC	2 cut(s)	197, 425
FokI	GGATG	4 cut(s)	316, 363, 486, 624
Fsp4HI	GCNGC	2 cut(s)	197, 425
FspBI	CTAG	5 cut(s)	41, 81, 272, 386, 413
GluI	GCNGC	2 cut(s)	197, 425
GsaI	CCCAGC	1 cut(s)	649
HaeIII	GGCC	1 cut(s)	196
Hin1II	CATG	2 cut(s)	302, 572
HinfI	GANTC	4 cut(s)	72, 98, 155, 179
HphI	GGTGA	6 cut(s)	98, 122, 485, 491, 556, 613
Hpy166II	GTNNAC	1 cut(s)	540
Hpy188I	TCNGA	4 cut(s)	103, 363, 421, 675
Hpy188III	TCNNGA	4 cut(s)	81, 134, 289, 299
Hpy8I	GTNNAC	1 cut(s)	540
HpyAV	CCTTC	2 cut(s)	131, 288
HpyCH4III	ACNGT	2 cut(s)	443, 525
HpyCH4IV	ACGT	2 cut(s)	229, 236
HpyF3I	CTNAG	1 cut(s)	420
HpySE526I	ACGT	2 cut(s)	229, 236
Hsp92II	CATG	2 cut(s)	302, 572
Ksp22I	TGATCA	1 cut(s)	295
Kzo9I	GATC	4 cut(s)	43, 130, 295, 363
LmnI	GCTCC	1 cut(s)	121
Lsp1109I	GCAGC	1 cut(s)	436
LweI	GCATC	3 cut(s)	103, 338, 665
MabI	ACCWGGT	1 cut(s)	558
MaeI	CTAG	5 cut(s)	41, 81, 272, 386, 413
MaeII	ACGT	2 cut(s)	229, 236
MaeIII	GTNAC	2 cut(s)	86, 562
MalI	GATC	4 cut(s)	45, 132, 297, 365
MboI	GATC	4 cut(s)	43, 130, 295, 363
MboII	GAAGA	3 cut(s)	326, 364, 442
MflI	RGATCY	3 cut(s)	43, 130, 363
MluCI	AATT	4 cut(s)	260, 580, 604, 626
MlyI	GAGTC	1 cut(s)	81
MmeI	TCCRAC	1 cut(s)	71
MnlI	CCTC	8 cut(s)	77, 180, 343, 391, 427, 475, 501, 669
MseI	TTAA	1 cut(s)	702
MslI	CAYNNNNRTG	1 cut(s)	552
MspA1I	CMGCKG	4 cut(s)	161, 427, 505, 518
MspR9I	CCNGG	2 cut(s)	560, 642
MvaI	CCWGG	2 cut(s)	560, 642
NdeII	GATC	4 cut(s)	43, 130, 295, 363
NlaIII	CATG	2 cut(s)	302, 572
NmuCI	GTSAC	2 cut(s)	86, 562
PaeR7I	CTCGAG	1 cut(s)	287
PagI	TCATGA	1 cut(s)	298
PfeI	GAWTC	3 cut(s)	98, 155, 179
PflFI	GACNNNGTC	1 cut(s)	560
PkrI	GCNGC	2 cut(s)	198, 426
PleI	GAGTC	1 cut(s)	80
PpsI	GAGTC	1 cut(s)	80
Ppu21I	YACGTR	1 cut(s)	237
PpuMI	RGGWCCY	1 cut(s)	275
Psp1406I	AACGTT	1 cut(s)	229
Psp5II	RGGWCCY	1 cut(s)	275
Psp6I	CCWGG	2 cut(s)	558, 640
PspEI	GGTNACC	1 cut(s)	562
PspFI	CCCAGC	1 cut(s)	645
PspGI	CCWGG	2 cut(s)	558, 640
PspPI	GGNCC	1 cut(s)	275
PspPPI	RGGWCCY	1 cut(s)	275
PsuI	RGATCY	3 cut(s)	43, 130, 363
PsyI	GACNNNGTC	1 cut(s)	560
PvuII	CAGCTG	2 cut(s)	161, 518
RsaI	GTAC	3 cut(s)	57, 239, 541
RsaNI	GTAC	3 cut(s)	56, 238, 540
RseI	CAYNNNNRTG	1 cut(s)	552
SaqAI	TTAA	1 cut(s)	702
SatI	GCNGC	2 cut(s)	197, 425
Sau3AI	GATC	4 cut(s)	43, 130, 295, 363
Sau96I	GGNCC	1 cut(s)	275
SchI	GAGTC	1 cut(s)	81
ScrFI	CCNGG	2 cut(s)	560, 642
SexAI	ACCWGGT	1 cut(s)	558
SfaNI	GCATC	3 cut(s)	103, 338, 665
Sfr274I	CTCGAG	1 cut(s)	287
SinI	GGWCC	1 cut(s)	275
SlaI	CTCGAG	1 cut(s)	287
SmiMI	CAYNNNNRTG	1 cut(s)	552
SmlI	CTYRAG	1 cut(s)	287
SmoI	CTYRAG	1 cut(s)	287
SnaBI	TACGTA	1 cut(s)	237
Sse9I	AATT	4 cut(s)	260, 580, 604, 626
SsiI	CCGC	3 cut(s)	197, 427, 503
SspI	AATATT	1 cut(s)	409
SspMI	CTAG	5 cut(s)	41, 81, 272, 386, 413
StyD4I	CCNGG	2 cut(s)	558, 640
StyI	CCWWGG	1 cut(s)	586
TaaI	ACNGT	2 cut(s)	443, 525
TaiI	ACGT	2 cut(s)	232, 239
TaqI	TCGA	1 cut(s)	288
TasI	AATT	4 cut(s)	260, 580, 604, 626
TauI	GCSGC	1 cut(s)	199
TfiI	GAWTC	3 cut(s)	98, 155, 179
Tru1I	TTAA	1 cut(s)	702
Tru9I	TTAA	1 cut(s)	702
TseFI	GTSAC	2 cut(s)	86, 562
TseI	GCWGC	1 cut(s)	424
Tsp45I	GTSAC	2 cut(s)	86, 562
TspDTI	ATGAA	1 cut(s)	405
TspGWI	ACGGA	1 cut(s)	38
Tth111I	GACNNNGTC	1 cut(s)	560
VpaK11BI	GGWCC	1 cut(s)	275
XapI	RAATTY	1 cut(s)	626
XbaI	TCTAGA	1 cut(s)	80
XhoI	CTCGAG	1 cut(s)	287
XspI	CTAG	5 cut(s)	41, 81, 272, 386, 413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.