MD13G1147500.v1.1

Craniofacial development protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009635
Physical Location & Seq
Reverse (-)
11534576 .. 11537423
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1147500.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGCAACCACCACCACAACCACCGCTCTCGGCGCCTCCAGTTCAGGTACTGCAGGTGAAAAGGATGGTTTACTAGTCGAGTCTACCAATGGAGCTGCTCCTCTTCCAAGCTTAGAGACAAAACCTGAACAAACAGAGGTAAAAGCTCGGGTAGATGCCATGTGGGAGCAAATGAATAAAGGAGTATCAAATAAGCAGTTCAAGGATTTATTAAAGAAGTCTTCCTCATCTGTGAATAAAACTCCCAAGAAGTCATCTAATAATTGGATGGGATATCTGGGCCTGGCACAAAAGAAGGCGGAGTCCCCTAAAGAAGATGCATCACAGAAGGGATCTAGTGTCATGAAGAACAGCTCCAGTGATAATGGTGTGCTGGATAAATCTCGTGACGAGGCCAGGAGGCTTGCTGCTGCTGCTCTTGTAGAAGTTAAGGATGCTGCAGTTGCAACATCTGGCAGAGGGAAAGTTGAGATCATGGAGGTTCGGGATTTTGCTGGTAAAGAAATTGAATATAAGAAGCTTGTTGATGCTGATTCAAAGGAAGCATCCGAAAAGGCAAAAGCTCCTGCATCTTCAGGTGTCGATGCTGTGCTCGAACGGATTAGGAAGAAACAAAAGCTCAGTGTGCTTGACAAAACGAAAAAGGATTGGGGGGAGTTTAAGGAAGAGAAAGGGTTGGAGGAGGAGTTGGAATCTTACAAAAAGAGTTCAAACCAATATTTGGATAAGGTAAACTTCTTGCAGCGAGCAGATTTCCGAGAGTTTGAACGGGAGAGGGATGCGCGCCTGGCCGTGCAGGCCAAAAGGAAACCAGATATGCGGGAGGAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCNT

Protein Analysis

278

Amino Acids

30.52

Weight (kDa)

9.13

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCNT PF07572 192 - 263 8.9e-26 Bucentaur or craniofacial development
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 721
AccBSI CCGCTC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 83
AccII CGCG 1 cut(s) 784
AciI CCGC 3 cut(s) 24, 299, 820
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 789
AcuI CTGAAG 1 cut(s) 558
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 721
AgsI TTSAA 5 cut(s) 202, 509, 537, 711, 767
AhlI ACTAGT 1 cut(s) 73
AjnI CCWGG 3 cut(s) 282, 395, 786
AluBI AGCT 7 cut(s) 95, 111, 146, 354, 520, 563, 619
AluI AGCT 7 cut(s) 95, 111, 146, 354, 520, 563, 619
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 340
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 4 cut(s) 280, 393, 789, 798
ApeKI GCWGC 6 cut(s) 95, 407, 410, 413, 437, 742
AspLEI GCGC 3 cut(s) 35, 784, 786
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 147
BanI GGYRCC 1 cut(s) 32
BauI CACGAG 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 6 cut(s) 82, 394, 397, 400, 424, 754
BccI CCATC 2 cut(s) 59, 262
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 3 cut(s) 284, 397, 788
BcoDI GTCTC 1 cut(s) 110
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 2 cut(s) 74, 336
BfmI CTRYAG 2 cut(s) 51, 438
BfoI RGCGCY 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 797
BisI GCNGC 6 cut(s) 96, 408, 411, 414, 438, 743
BlsI GCNGC 6 cut(s) 97, 409, 412, 415, 439, 744
Bme1390I CCNGG 3 cut(s) 284, 397, 788
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 34, 828
BmrFI CCNGG 3 cut(s) 284, 397, 788
BmsI GCATC 9 cut(s) 145, 307, 329, 424, 517, 554, 574, 578, 769
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 22, 340
BsaHI GRCGYC 1 cut(s) 33
BsaXI ACNNNNNCTCC 2 cut(s) 671, 701
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse1I ACTGG 2 cut(s) 39, 357
BseBI CCWGG 3 cut(s) 284, 397, 788
BseGI GGATG 5 cut(s) 70, 273, 439, 545, 784
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMII CTCAG 1 cut(s) 634
BseNI ACTGG 2 cut(s) 39, 357
BsePI GCGCGC 1 cut(s) 782
BseRI GAGGAG 3 cut(s) 90, 695, 698
BseXI GCAGC 6 cut(s) 82, 394, 397, 400, 424, 754
BsgI GTGCAG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 784
BshFI GGCC 4 cut(s) 282, 395, 791, 800
BshNI GGYRCC 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 4 cut(s) 282, 395, 791, 800
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 594
Bsp143I GATC 2 cut(s) 332, 471
BspACI CCGC 3 cut(s) 24, 299, 820
BspANI GGCC 4 cut(s) 282, 395, 791, 800
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 784
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 34, 828
BspMAI CTGCAG 2 cut(s) 55, 442
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 26
BsrI ACTGG 2 cut(s) 39, 357
BssHII GCGCGC 1 cut(s) 782
BssMI GATC 2 cut(s) 332, 471
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 384
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 284, 397, 788
Bst6I CTCTTC 2 cut(s) 108, 660
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 4 cut(s) 405, 747, 784, 798
BstDEI CTNAG 2 cut(s) 112, 620
BstF5I GGATG 5 cut(s) 70, 273, 439, 545, 784
BstFNI CGCG 1 cut(s) 784
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 3 cut(s) 35, 784, 786
BstKTI GATC 2 cut(s) 335, 474
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 332, 471
BstMWI GCNNNNNNNGC 7 cut(s) 32, 413, 443, 625, 788, 797, 826
BstNI CCWGG 3 cut(s) 284, 397, 788
BstSCI CCNGG 3 cut(s) 282, 395, 786
BstSFI CTRYAG 2 cut(s) 51, 438
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 6 cut(s) 82, 394, 397, 400, 424, 754
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 4 cut(s) 282, 395, 791, 800
BtsCI GGATG 5 cut(s) 70, 273, 439, 545, 784
BtsIMutI CAGTG 2 cut(s) 364, 628
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 4 cut(s) 405, 747, 784, 798
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 342
CfoI GCGC 3 cut(s) 35, 784, 786
Cfr13I GGNCC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 48
CviAII CATG 3 cut(s) 160, 343, 475
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 112, 620
DinI GGCGCC 1 cut(s) 34
DpnI GATC 2 cut(s) 334, 473
DpnII GATC 2 cut(s) 332, 471
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 2 cut(s) 108, 660
EarI CTCTTC 2 cut(s) 108, 660
EciI GGCGGA 1 cut(s) 314
Eco32I GATATC 1 cut(s) 275
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 147
EcoRII CCWGG 3 cut(s) 282, 395, 786
EcoRV GATATC 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 322
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
FaeI CATG 3 cut(s) 163, 346, 478
FaiI YATR 6 cut(s) 161, 344, 476, 513, 818, 832
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 1 cut(s) 289
FatI CATG 3 cut(s) 159, 342, 474
FauI CCCGC 1 cut(s) 813
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 6 cut(s) 96, 408, 411, 414, 438, 743
FokI GGATG 5 cut(s) 77, 280, 446, 532, 791
Fsp4HI GCNGC 6 cut(s) 96, 408, 411, 414, 438, 743
FspBI CTAG 2 cut(s) 74, 336
GlaI GCGC 3 cut(s) 34, 783, 785
GluI GCNGC 6 cut(s) 96, 408, 411, 414, 438, 743
GsuI CTGGAG 2 cut(s) 22, 340
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 4 cut(s) 282, 395, 791, 800
HhaI GCGC 3 cut(s) 35, 784, 786
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 163, 346, 478
Hin6I GCGC 3 cut(s) 33, 782, 784
HinP1I GCGC 3 cut(s) 33, 782, 784
HindIII AAGCTT 2 cut(s) 109, 518
HinfI GANTC 4 cut(s) 80, 302, 533, 692
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 3 cut(s) 71, 84, 733
Hpy188I TCNGA 2 cut(s) 550, 758
Hpy188III TCNNGA 3 cut(s) 343, 386, 485
Hpy8I GTNNAC 3 cut(s) 71, 84, 733
HpyAV CCTTC 2 cut(s) 289, 322
HpyCH4V TGCA 7 cut(s) 53, 320, 440, 446, 569, 742, 796
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 413, 443, 625, 788, 797, 826
HpyF3I CTNAG 2 cut(s) 112, 620
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 163, 346, 478
HspAI GCGC 3 cut(s) 33, 782, 784
KasI GGCGCC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 332, 471
LmnI GCTCC 6 cut(s) 92, 103, 166, 359, 568, 826
Lsp1109I GCAGC 6 cut(s) 82, 394, 397, 400, 424, 754
LweI GCATC 9 cut(s) 145, 307, 329, 424, 517, 554, 574, 578, 769
MaeI CTAG 2 cut(s) 74, 336
MaeIII GTNAC 1 cut(s) 386
MalI GATC 2 cut(s) 334, 473
MbiI CCGCTC 1 cut(s) 26
MboI GATC 2 cut(s) 332, 471
MboII GAAGA 7 cut(s) 95, 213, 326, 358, 564, 619, 677
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 2 cut(s) 262, 504
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 2 cut(s) 89, 311
MmeI TCCRAC 2 cut(s) 657, 669
Mph1103I ATGCAT 1 cut(s) 322
MseI TTAA 3 cut(s) 212, 429, 660
MspR9I CCNGG 3 cut(s) 284, 397, 788
MvaI CCWGG 3 cut(s) 284, 397, 788
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 7 cut(s) 32, 413, 443, 625, 788, 797, 826
NarI GGCGCC 1 cut(s) 33
NdeII GATC 2 cut(s) 332, 471
NlaIII CATG 3 cut(s) 163, 346, 478
NlaIV GGNNCC 2 cut(s) 34, 828
NmeAIII GCCGAG 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 322
PagI TCATGA 1 cut(s) 342
PaqCI CACCTGC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 533, 692
PflMI CCANNNNNTGG 1 cut(s) 721
PkrI GCNGC 6 cut(s) 97, 409, 412, 415, 439, 744
PleI GAGTC 2 cut(s) 88, 310
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 2 cut(s) 88, 310
Psp6I CCWGG 3 cut(s) 282, 395, 786
PspGI CCWGG 3 cut(s) 282, 395, 786
PspN4I GGNNCC 2 cut(s) 34, 828
PspPI GGNCC 1 cut(s) 280
PstI CTGCAG 2 cut(s) 55, 442
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 332
PteI GCGCGC 1 cut(s) 782
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 212, 429, 660
SatI GCNGC 6 cut(s) 96, 408, 411, 414, 438, 743
Sau3AI GATC 2 cut(s) 332, 471
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 2 cut(s) 89, 311
ScrFI CCNGG 3 cut(s) 284, 397, 788
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 9 cut(s) 145, 307, 329, 424, 517, 554, 574, 578, 769
SfcI CTRYAG 2 cut(s) 51, 438
SfiI GGCCNNNNNGGCC 1 cut(s) 797
SfoI GGCGCC 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 73
Sse9I AATT 2 cut(s) 262, 504
SsiI CCGC 3 cut(s) 24, 299, 820
SspDI GGCGCC 1 cut(s) 32
SspI AATATT 1 cut(s) 719
SspMI CTAG 2 cut(s) 74, 336
StyD4I CCNGG 3 cut(s) 282, 395, 786
TaqI TCGA 3 cut(s) 78, 582, 594
TasI AATT 2 cut(s) 262, 504
TfiI GAWTC 2 cut(s) 533, 692
Tru1I TTAA 3 cut(s) 212, 429, 660
Tru9I TTAA 3 cut(s) 212, 429, 660
TscAI CASTG 2 cut(s) 364, 628
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 6 cut(s) 95, 407, 410, 413, 437, 742
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 188, 359
TspGWI ACGGA 1 cut(s) 613
TspRI CASTG 2 cut(s) 364, 628
Van91I CCANNNNNTGG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 83
XspI CTAG 2 cut(s) 74, 336
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.