MD13G1152100.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008465
Physical Location & Seq
Reverse (-)
11911156 .. 11914999
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1152100.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGCTTCAGGCATTGATGAATCTCCTCTCTCTTTGCTGGAAGCCATTTGGGCGCAGCGACGGTGAGGGACTCGGCTCTGTCGGAATCGTCGGCGCTGCGAACATCGCAGGCGGCGGTGGAGGACGAGATGGCAAAGATAATTTGCTTTGGTTCCGAGATTTCGGAAAGTACGCGTGGGGGGATTTCTCCATGGCGGTGGTCCAAGCCAATCAGGTGCTCGAGGACCAGAGCCAGATCGAGTCTGGACCCTTTGGAACCTTCGTCGGCGTATACGACGGACATGGCGGCCCGGAGACCGCTCGATACGTTTGCGACAATTTATTTAAACATTTCCAAGCAATATCAGCGGAGTCCGAAGGTGTTGTGACGGCAGAGACTATCCAAAATGCTTTTCTCCGAACAGAGGAAGGGTTTACTGCCCTTGTTTCGGAGCTGTGGGGTACTAGACCCCAAGTAGCAACTGTCGGGTCATGCTGTCTGGTTGGAGTAATATGTCAGGGGACCCTATTTGTGGCAAACTTGGGAGATTCACGGTGTGTGTTGGGGAAGAAAGTGGGCAACACTGGGGAAATTGCTGCGATCCAGTTGTCCACTGAACACAATGCAAATCTCGAGGAGGTTAGGCGTGAGCTGAAAGATTTGCACCCACATGATCCTGAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGGATAATCCAGGTATCTAAATCTATAGGTGACGTATATATGAAACATGCACGGTTTAACAGGGAGCCAATTAGTGCAAAATTCAGACTTCCCGAACCAATGACCATGCCTATCTTGTCTGCCGAACCAACCATTCTTTCTCATCCCCTCCATCCAAATGATTCTTTCCTTATATTTGCTTCTGATGGTCTATGGGAACACTTGAGTAATGAAAAAGCTGTTGAGATTGTCCACAATCATCCACATGCAGGAAGTGCCAAGCGGCTTGTTAAGACGGCTCTCCAAGAAGCTGCAAGGAAAAGAGAGATGCGATATTCAGATCTTCGGAAAATTGACAAGAAGGTCAGACGCCATTTTCACGATGATATAACTGTCATTGTTTTATTCTTAAACCATGACCTAATATCCAGAGGCACAGCGCGAGAGCCCCCAGTATCAATAAAAGGTGCCCGTGACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

386

Amino Acids

42.38

Weight (kDa)

6.79

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 331 1.3e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
AccB1I GGYRCC 1 cut(s) 1142
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 173, 1117
AciI CCGC 7 cut(s) 111, 114, 194, 285, 297, 347, 958
AclWI GGATC 2 cut(s) 574, 647
AcsI RAATTY 1 cut(s) 776
AcyI GRCGYC 1 cut(s) 1045
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 2 cut(s) 170, 442
AfiI CCNNNNNNNGG 4 cut(s) 403, 427, 511, 944
AflIII ACRYGT 1 cut(s) 171
AjnI CCWGG 1 cut(s) 705
AjuI GAANNNNNNNTTGG 4 cut(s) 375, 407, 817, 849
AluBI AGCT 4 cut(s) 433, 631, 914, 986
AluI AGCT 4 cut(s) 433, 631, 914, 986
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 287, 368
AlwI GGATC 2 cut(s) 574, 647
Ama87I CYCGRG 2 cut(s) 218, 611
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 4 cut(s) 54, 95, 575, 986
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 3 cut(s) 54, 95, 1117
AspS9I GGNCC 5 cut(s) 199, 223, 245, 287, 501
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 74, 737
AvaI CYCGRG 2 cut(s) 218, 611
AvaII GGWCC 4 cut(s) 199, 223, 245, 501
BaeGI GKGCMC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 1142
BanII GRGCYC 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 4 cut(s) 66, 82, 562, 973
BccI CCATC 3 cut(s) 122, 855, 875
BceAI ACGGC 2 cut(s) 384, 987
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciT130I CCWGG 1 cut(s) 707
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 287, 368
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 720
BfoI RGCGCY 1 cut(s) 96
BglI GCCNNNNNGGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 1015
BisI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
BlsI GCNGC 7 cut(s) 56, 97, 113, 287, 577, 960, 988
Bme1390I CCNGG 2 cut(s) 290, 707
Bme18I GGWCC 4 cut(s) 199, 223, 245, 501
BmeT110I CYCGRG 2 cut(s) 218, 611
BmgT120I GGNCC 5 cut(s) 199, 223, 245, 287, 501
BmiI GGNNCC 7 cut(s) 152, 247, 256, 502, 503, 762, 1144
BmrFI CCNGG 2 cut(s) 290, 707
BmrI ACTGGG 2 cut(s) 573, 1121
BmsI GCATC 1 cut(s) 993
BmuI ACTGGG 2 cut(s) 573, 1121
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 1045
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 4 cut(s) 403, 427, 511, 944
Bse1I ACTGG 3 cut(s) 568, 583, 1127
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 3 cut(s) 838, 847, 934
BseLI CCNNNNNNNGG 4 cut(s) 403, 427, 511, 944
BseNI ACTGG 3 cut(s) 568, 583, 1127
BseRI GAGGAG 2 cut(s) 14, 629
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 4 cut(s) 66, 82, 562, 973
Bsh1236I CGCG 2 cut(s) 173, 1117
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 1142
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 218, 611
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 2 cut(s) 81, 514
BslI CCNNNNNNNGG 4 cut(s) 403, 427, 511, 944
BsmAI GTCTC 2 cut(s) 287, 368
BsmFI GGGAC 2 cut(s) 81, 514
BsnI GGCC 1 cut(s) 288
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 218, 611
Bsp1286I GDGCHC 3 cut(s) 219, 1125, 1147
Bsp143I GATC 4 cut(s) 234, 579, 652, 1015
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 7 cut(s) 111, 114, 194, 285, 297, 347, 958
BspANI GGCC 1 cut(s) 288
BspFNI CGCG 2 cut(s) 173, 1117
BspLI GGNNCC 7 cut(s) 152, 247, 256, 502, 503, 762, 1144
BspPI GGATC 2 cut(s) 574, 647
BspT107I GGYRCC 1 cut(s) 1142
BspTNI GGTCTC 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 3 cut(s) 568, 583, 1127
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 4 cut(s) 234, 579, 652, 1015
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 1045
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 5 cut(s) 62, 463, 534, 750, 1069
BstACI GRCGYC 1 cut(s) 1045
BstAPI GCANNNNNTGC 1 cut(s) 950
BstC8I GCNNGC 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 838, 847, 934
BstFNI CGCG 2 cut(s) 173, 1117
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 3 cut(s) 54, 95, 1117
BstKTI GATC 4 cut(s) 237, 582, 655, 1018
BstMAI GTCTC 2 cut(s) 287, 368
BstMBI GATC 4 cut(s) 234, 579, 652, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 49, 104, 344, 950
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 2 cut(s) 746, 944
BstSCI CCNGG 2 cut(s) 288, 705
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 1147
BstUI CGCG 2 cut(s) 173, 1117
BstV1I GCAGC 4 cut(s) 66, 82, 562, 973
BstX2I RGATCY 1 cut(s) 1015
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 1015
BstZ17I GTATAC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 3 cut(s) 838, 847, 934
BtsIMutI CAGTG 3 cut(s) 561, 591, 1153
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 3 cut(s) 54, 95, 1117
Cfr13I GGNCC 5 cut(s) 199, 223, 245, 287, 501
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 2 cut(s) 169, 441
CviAII CATG 9 cut(s) 190, 281, 471, 650, 677, 743, 802, 941, 1091
CviQI GTAC 2 cut(s) 169, 441
DpnI GATC 4 cut(s) 236, 581, 654, 1017
DpnII GATC 4 cut(s) 234, 579, 652, 1015
DraI TTTAAA 2 cut(s) 325, 672
DraIII CACNNNGTG 1 cut(s) 536
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 4 cut(s) 199, 223, 245, 501
Eco88I CYCGRG 2 cut(s) 218, 611
EcoO109I RGGNCCY 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 9 cut(s) 193, 284, 474, 653, 680, 746, 805, 944, 1094
FaqI GGGAC 2 cut(s) 81, 514
FatI CATG 9 cut(s) 189, 280, 470, 649, 676, 742, 801, 940, 1090
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
FokI GGATG 3 cut(s) 825, 834, 921
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
FspBI CTAG 1 cut(s) 444
GlaI GCGC 3 cut(s) 53, 94, 1116
GluI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 288
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 1053
HhaI GCGC 3 cut(s) 54, 95, 1117
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 9 cut(s) 193, 284, 474, 653, 680, 746, 805, 944, 1094
Hin6I GCGC 3 cut(s) 52, 93, 1115
HinP1I GCGC 3 cut(s) 52, 93, 1115
HinfI GANTC 7 cut(s) 19, 69, 84, 239, 350, 527, 857
HpaII CCGG 1 cut(s) 290
HphI GGTGA 2 cut(s) 74, 737
Hpy166II GTNNAC 4 cut(s) 271, 414, 591, 928
Hpy188III TCNNGA 6 cut(s) 243, 611, 656, 788, 1055, 1104
Hpy8I GTNNAC 4 cut(s) 271, 414, 591, 928
Hpy99I CGWCG 4 cut(s) 62, 92, 266, 278
HpyAV CCTTC 4 cut(s) 268, 350, 401, 1030
HpyCH4III ACNGT 5 cut(s) 62, 463, 534, 750, 1069
HpyCH4IV ACGT 2 cut(s) 306, 729
HpyCH4V TGCA 6 cut(s) 605, 643, 746, 773, 944, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 104, 344, 950
HpySE526I ACGT 2 cut(s) 306, 729
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 9 cut(s) 193, 284, 474, 653, 680, 746, 805, 944, 1094
HspAI GCGC 3 cut(s) 52, 93, 1115
KflI GGGWCCC 1 cut(s) 501
Kzo9I GATC 4 cut(s) 234, 579, 652, 1015
LmnI GCTCC 2 cut(s) 430, 760
Lsp1109I GCAGC 4 cut(s) 66, 82, 562, 973
LweI GCATC 1 cut(s) 993
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 306, 729
MaeIII GTNAC 3 cut(s) 364, 725, 1148
MalI GATC 4 cut(s) 236, 581, 654, 1017
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 234, 579, 652, 1015
MboII GAAGA 2 cut(s) 559, 1010
MflI RGATCY 1 cut(s) 1015
MhlI GDGCHC 3 cut(s) 219, 1125, 1147
MluCI AATT 7 cut(s) 139, 316, 570, 660, 765, 776, 1026
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 3 cut(s) 63, 248, 359
MmeI TCCRAC 2 cut(s) 61, 463
MnlI CCTC 9 cut(s) 35, 58, 113, 214, 397, 607, 610, 854, 1100
MseI TTAA 5 cut(s) 324, 671, 753, 966, 1085
MslI CAYNNNNRTG 4 cut(s) 194, 648, 852, 939
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 290, 707
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 2 cut(s) 173, 1117
MwoI GCNNNNNNNGC 4 cut(s) 49, 104, 344, 950
NciI CCSGG 1 cut(s) 290
NcoI CCATGG 1 cut(s) 189
NdeII GATC 4 cut(s) 234, 579, 652, 1015
NlaIII CATG 9 cut(s) 193, 284, 474, 653, 680, 746, 805, 944, 1094
NlaIV GGNNCC 7 cut(s) 152, 247, 256, 502, 503, 762, 1144
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 3 cut(s) 364, 725, 1148
NspI RCATGY 2 cut(s) 746, 944
PaeR7I CTCGAG 2 cut(s) 218, 611
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 4 cut(s) 19, 84, 527, 857
PkrI GCNGC 7 cut(s) 56, 97, 113, 287, 577, 960, 988
PleI GAGTC 3 cut(s) 63, 247, 358
PpsI GAGTC 3 cut(s) 63, 247, 358
PpuMI RGGWCCY 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 7 cut(s) 152, 247, 256, 502, 503, 762, 1144
PspPI GGNCC 5 cut(s) 199, 223, 245, 287, 501
PspPPI RGGWCCY 1 cut(s) 501
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 1 cut(s) 1015
RsaI GTAC 2 cut(s) 170, 442
RsaNI GTAC 2 cut(s) 169, 441
RseI CAYNNNNRTG 4 cut(s) 194, 648, 852, 939
SaqAI TTAA 5 cut(s) 324, 671, 753, 966, 1085
SatI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
Sau3AI GATC 4 cut(s) 234, 579, 652, 1015
Sau96I GGNCC 5 cut(s) 199, 223, 245, 287, 501
SchI GAGTC 3 cut(s) 63, 248, 359
ScrFI CCNGG 2 cut(s) 290, 707
SduI GDGCHC 3 cut(s) 219, 1125, 1147
SfaNI GCATC 1 cut(s) 993
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 2 cut(s) 218, 611
SinI GGWCC 4 cut(s) 199, 223, 245, 501
SlaI CTCGAG 2 cut(s) 218, 611
SmiMI CAYNNNNRTG 4 cut(s) 194, 648, 852, 939
SmlI CTYRAG 3 cut(s) 218, 611, 898
SmoI CTYRAG 3 cut(s) 218, 611, 898
Sse9I AATT 7 cut(s) 139, 316, 570, 660, 765, 776, 1026
SsiI CCGC 7 cut(s) 111, 114, 194, 285, 297, 347, 958
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 288, 705
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 62, 463, 534, 750, 1069
TaiI ACGT 2 cut(s) 309, 732
TaqI TCGA 4 cut(s) 219, 237, 301, 612
TasI AATT 7 cut(s) 139, 316, 570, 660, 765, 776, 1026
TauI GCSGC 3 cut(s) 114, 288, 961
TfiI GAWTC 4 cut(s) 19, 84, 527, 857
Tru1I TTAA 5 cut(s) 324, 671, 753, 966, 1085
Tru9I TTAA 5 cut(s) 324, 671, 753, 966, 1085
TscAI CASTG 2 cut(s) 568, 598
TseFI GTSAC 3 cut(s) 364, 725, 1148
TseI GCWGC 4 cut(s) 54, 95, 575, 986
Tsp45I GTSAC 3 cut(s) 364, 725, 1148
TspDTI ATGAA 3 cut(s) 32, 752, 921
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 568, 598
VpaK11BI GGWCC 4 cut(s) 199, 223, 245, 501
XapI RAATTY 1 cut(s) 776
XceI RCATGY 2 cut(s) 746, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 2 cut(s) 218, 611
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.