Rh4CG278100

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008465
Physical Location & Seq
Forward (+)
53534401 .. 53538298
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG278100.1

Sequence Viewer

Length: 1161 bp
ATGCTTCATGCATTGATGAATCTTCTCTCGCTGTGTTGGAAGCCATTTGGGCGCGGCGACGGTGAGGGAGGAGTCGACTCTATCGGAATTGTTGGCGCCAATTTAAACGGCGGCGGCGGTGGAGGAAGAGATGGCAAAGATAGCTTGCTTTGGTTCCGAGATATCGGAAAGTACGCCTGGGGAGATTTCTCCATGGCTGTGGTTCAGGCCAATCAGGTCCTCGAGGACCAGGGGCAGATCGAGTCCGGCCCCTTTGGGACCTTCGTCGGCGTCTATGATGGCCACGGTGGCCCGGAGGCCGCTCAATACGTCTGCGATAATCTATTTAGACATTTTCAAGCAGTATCAGCGGAGACGGAGGATGTTGTGACTGCAGAGACCATTCAAAATGCTTTTCGTCAGACAGAGGAAGGATTTACGGCCCTTGTTTCTGAGTTGTGGAGTACTAAACCCAATTTAGCAACTGTAGGAACATGCTGTCTGGTTGGGGTGATATGTCAGGGGACCCTATTTGTGGCAAGTCTTGGAGATTCCCGTCTTGTTTTGGGGAAGAAAGTGGGCAACACTGGGGAAGTTGCTGCTATTCAGTTATCCACTGAACACAATGCGAATCTCGATGAGATTAGGTACGAGCTAAAAGAGCTGCACCCACATGATTCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGAATCATCCAGGTTTCTAGATCTATAGGTGATGTATATATGAAACATGCACAGTTTAACAGGGAGCCAATTGCTGCAAAATTCCGACTTCCTGAACCAATGAACATGCCTATCATGTCTGCCACTCCAACTATTCTTTCTCATGCTCTGCATCCAAATGATTCTTTTCTTATCTTTGCGTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAGATTGTCCACAAGCATCCTCATGCAGGAAGTGCCAAAAGGCTTGTTAAGACAGCGCTCCAAGAAGCTGCAAGAAAACGAGAAATGCGTTATTCAGATCTTCGGAAAATAGACAAGAAAGTGCGGCGCCATTTTCATGATGATATAACTGTTATTGTTTTATTCTTGAACCATGACCTAATCTCCAGAGGCACAGCGCAAGAACCCCCAGTCTCAATAAGAAGTGGTCTTGATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

386

Amino Acids

42.4

Weight (kDa)

6.22

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 87 - 332 4.2e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 221
AccB1I GGYRCC 2 cut(s) 95, 1047
AccBSI CCGCTC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 54
AciI CCGC 7 cut(s) 54, 111, 114, 117, 300, 350, 1045
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 779
AcyI GRCGYC 3 cut(s) 96, 270, 1048
AfaI GTAC 3 cut(s) 173, 445, 629
AfeI AGCGCT 1 cut(s) 978
AfiI CCNNNNNNNGG 2 cut(s) 514, 947
AgsI TTSAA 3 cut(s) 338, 386, 1090
AjnI CCWGG 3 cut(s) 176, 228, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 820, 852
AluBI AGCT 5 cut(s) 144, 634, 643, 917, 989
AluI AGCT 5 cut(s) 144, 634, 643, 917, 989
Alw26I GTCTC 3 cut(s) 347, 371, 1138
Ama87I CYCGRG 1 cut(s) 221
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 6 cut(s) 207, 247, 280, 289, 297, 420
ApeKI GCWGC 4 cut(s) 578, 643, 773, 989
ApoI RAATTY 1 cut(s) 779
AspLEI GCGC 5 cut(s) 54, 98, 979, 1050, 1120
AspS9I GGNCC 7 cut(s) 217, 226, 248, 258, 290, 421, 504
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 74, 502, 740
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 4 cut(s) 217, 226, 258, 504
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 2 cut(s) 95, 1047
BbvI GCAGC 4 cut(s) 565, 630, 760, 976
BccI CCATC 3 cut(s) 125, 272, 878
BceAI ACGGC 2 cut(s) 124, 435
BciT130I CCWGG 3 cut(s) 178, 230, 710
BclI TGATCA 1 cut(s) 1153
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 347, 371, 1138
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 3 cut(s) 372, 465, 723
BfoI RGCGCY 3 cut(s) 99, 980, 1051
BglI GCCNNNNNGGC 2 cut(s) 49, 288
BglII AGATCT 2 cut(s) 719, 1018
BisI GCNGC 9 cut(s) 55, 112, 115, 300, 579, 644, 774, 990, 1046
BlsI GCNGC 9 cut(s) 56, 113, 116, 301, 580, 645, 775, 991, 1047
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 4 cut(s) 178, 230, 293, 710
Bme18I GGWCC 4 cut(s) 217, 226, 258, 504
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 7 cut(s) 217, 226, 248, 258, 290, 421, 504
BmiI GGNNCC 8 cut(s) 97, 155, 250, 259, 505, 506, 765, 1049
BmrFI CCNGG 4 cut(s) 178, 230, 293, 710
BmrI ACTGGG 2 cut(s) 576, 1124
BmsI GCATC 2 cut(s) 859, 946
BmuI ACTGGG 2 cut(s) 576, 1124
BoxI GACNNNNGTC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 1090
BpuEI CTTGAG 1 cut(s) 922
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 3 cut(s) 96, 270, 1048
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 4 cut(s) 177, 192, 229, 283
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 947
Bse1I ACTGG 2 cut(s) 571, 1130
BseBI CCWGG 3 cut(s) 178, 230, 710
BseDI CCNNGG 4 cut(s) 177, 192, 229, 283
BseGI GGATG 4 cut(s) 367, 705, 850, 937
BseLI CCNNNNNNNGG 2 cut(s) 514, 947
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 2 cut(s) 571, 1130
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 4 cut(s) 565, 630, 760, 976
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 6 cut(s) 209, 249, 282, 291, 299, 422
BshNI GGYRCC 2 cut(s) 95, 1047
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 2 cut(s) 246, 293
BslFI GGGAC 2 cut(s) 271, 517
BslI CCNNNNNNNGG 2 cut(s) 514, 947
BsmAI GTCTC 3 cut(s) 347, 371, 1138
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 271, 517
BsnI GGCC 6 cut(s) 209, 249, 282, 291, 299, 422
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 221
Bsp143I GATC 4 cut(s) 237, 719, 1018, 1153
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 7 cut(s) 54, 111, 114, 117, 300, 350, 1045
BspANI GGCC 6 cut(s) 209, 249, 282, 291, 299, 422
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 1057
BspLI GGNNCC 8 cut(s) 97, 155, 250, 259, 505, 506, 765, 1049
BspMAI CTGCAG 1 cut(s) 376
BspT107I GGYRCC 2 cut(s) 95, 1047
BspTNI GGTCTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 302
BsrI ACTGG 2 cut(s) 571, 1130
BssECI CCNNGG 4 cut(s) 177, 192, 229, 283
BssMI GATC 4 cut(s) 237, 719, 1018, 1153
BssNI GRCGYC 3 cut(s) 96, 270, 1048
BssT1I CCWWGG 1 cut(s) 192
Bst2UI CCWGG 3 cut(s) 178, 230, 710
Bst4CI ACNGT 5 cut(s) 62, 287, 466, 753, 1072
Bst6I CTCTTC 1 cut(s) 121
BstACI GRCGYC 3 cut(s) 96, 270, 1048
BstAPI GCANNNNNTGC 1 cut(s) 953
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 2 cut(s) 192, 283
BstENI CCTNNNNNAGG 1 cut(s) 945
BstF5I GGATG 4 cut(s) 367, 705, 850, 937
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 3 cut(s) 99, 980, 1051
BstHHI GCGC 5 cut(s) 54, 98, 979, 1050, 1120
BstKTI GATC 4 cut(s) 240, 722, 1021, 1156
BstMAI GTCTC 3 cut(s) 347, 371, 1138
BstMBI GATC 4 cut(s) 237, 719, 1018, 1153
BstMWI GCNNNNNNNGC 6 cut(s) 49, 141, 288, 347, 640, 953
BstNI CCWGG 3 cut(s) 178, 230, 710
BstNSI RCATGY 3 cut(s) 477, 749, 808
BstPAI GACNNNNGTC 1 cut(s) 263
BstSCI CCNGG 4 cut(s) 176, 228, 291, 708
BstSFI CTRYAG 3 cut(s) 372, 465, 723
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 4 cut(s) 565, 630, 760, 976
BstX2I RGATCY 2 cut(s) 719, 1018
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 719, 1018
BsuRI GGCC 6 cut(s) 209, 249, 282, 291, 299, 422
BtgI CCRYGG 2 cut(s) 192, 283
BtsCI GGATG 4 cut(s) 367, 705, 850, 937
BtsIMutI CAGTG 2 cut(s) 564, 594
Cac8I GCNNGC 1 cut(s) 146
CciI TCATGA 1 cut(s) 1057
CfoI GCGC 5 cut(s) 54, 98, 979, 1050, 1120
Cfr13I GGNCC 7 cut(s) 217, 226, 248, 258, 290, 421, 504
CseI GACGC 2 cut(s) 259, 867
Csp6I GTAC 3 cut(s) 172, 444, 628
CviQI GTAC 3 cut(s) 172, 444, 628
DdeI CTNAG 1 cut(s) 432
DinI GGCGCC 2 cut(s) 97, 1049
DpnI GATC 4 cut(s) 239, 721, 1020, 1155
DpnII GATC 4 cut(s) 237, 719, 1018, 1153
DraI TTTAAA 2 cut(s) 105, 675
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 4 cut(s) 217, 226, 258, 504
Eco47III AGCGCT 1 cut(s) 978
Eco88I CYCGRG 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 945
EcoO109I RGGNCCY 3 cut(s) 217, 258, 504
EcoRII CCWGG 3 cut(s) 176, 228, 708
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 13
EgeI GGCGCC 2 cut(s) 97, 1049
EheI GGCGCC 2 cut(s) 97, 1049
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 347
FaqI GGGAC 2 cut(s) 271, 517
FbaI TGATCA 1 cut(s) 1153
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 9 cut(s) 55, 112, 115, 300, 579, 644, 774, 990, 1046
FokI GGATG 4 cut(s) 374, 692, 837, 924
Fsp4HI GCNGC 9 cut(s) 55, 112, 115, 300, 579, 644, 774, 990, 1046
FspBI CTAG 1 cut(s) 717
GlaI GCGC 5 cut(s) 53, 97, 978, 1049, 1119
GluI GCNGC 9 cut(s) 55, 112, 115, 300, 579, 644, 774, 990, 1046
GsuI CTGGAG 1 cut(s) 1090
HaeII RGCGCY 3 cut(s) 99, 980, 1051
HaeIII GGCC 6 cut(s) 209, 249, 282, 291, 299, 422
HapII CCGG 2 cut(s) 246, 293
HgaI GACGC 2 cut(s) 259, 867
HhaI GCGC 5 cut(s) 54, 98, 979, 1050, 1120
Hin1I GRCGYC 3 cut(s) 96, 270, 1048
Hin6I GCGC 5 cut(s) 52, 96, 977, 1048, 1118
HinP1I GCGC 5 cut(s) 52, 96, 977, 1048, 1118
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 9 cut(s) 19, 72, 77, 242, 530, 610, 656, 702, 860
HpaII CCGG 2 cut(s) 246, 293
HphI GGTGA 3 cut(s) 74, 502, 740
Hpy166II GTNNAC 2 cut(s) 76, 931
Hpy188I TCNGA 9 cut(s) 86, 158, 167, 402, 433, 785, 883, 1018, 1026
Hpy188III TCNNGA 7 cut(s) 614, 717, 791, 1058, 1087, 1107, 1151
Hpy8I GTNNAC 2 cut(s) 76, 931
Hpy99I CGWCG 2 cut(s) 62, 269
HpyAV CCTTC 2 cut(s) 271, 404
HpyCH4III ACNGT 5 cut(s) 62, 287, 466, 753, 1072
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 8 cut(s) 11, 374, 646, 749, 776, 850, 947, 992
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 141, 288, 347, 640, 953
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 3 cut(s) 96, 270, 1048
HspAI GCGC 5 cut(s) 52, 96, 977, 1048, 1118
KasI GGCGCC 2 cut(s) 95, 1047
KflI GGGWCCC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 1153
Kzo9I GATC 4 cut(s) 237, 719, 1018, 1153
LmnI GCTCC 2 cut(s) 763, 984
Lsp1109I GCAGC 4 cut(s) 565, 630, 760, 976
LweI GCATC 2 cut(s) 859, 946
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 367
MalI GATC 4 cut(s) 239, 721, 1020, 1155
MbiI CCGCTC 1 cut(s) 302
MboI GATC 4 cut(s) 237, 719, 1018, 1153
MboII GAAGA 4 cut(s) 14, 138, 562, 1013
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 719, 1018
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 6 cut(s) 87, 100, 454, 663, 768, 779
MluNI TGGCCA 1 cut(s) 282
Mly113I GGCGCC 2 cut(s) 96, 1048
MlyI GAGTC 3 cut(s) 71, 81, 251
MmeI TCCRAC 3 cut(s) 17, 808, 851
Mox20I TGGCCA 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 1 cut(s) 282
MseI TTAA 4 cut(s) 104, 674, 756, 969
MslI CAYNNNNRTG 5 cut(s) 197, 651, 855, 942, 1056
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 2 cut(s) 246, 293
MspR9I CCNGG 4 cut(s) 178, 230, 293, 710
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 3 cut(s) 178, 230, 710
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 6 cut(s) 49, 141, 288, 347, 640, 953
NarI GGCGCC 2 cut(s) 96, 1048
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 192
NdeII GATC 4 cut(s) 237, 719, 1018, 1153
NlaIV GGNNCC 8 cut(s) 97, 155, 250, 259, 505, 506, 765, 1049
NmuCI GTSAC 1 cut(s) 367
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 477, 749, 808
PaeR7I CTCGAG 1 cut(s) 221
PagI TCATGA 1 cut(s) 1057
PcsI WCGNNNNNNNCGW 1 cut(s) 1006
PfeI GAWTC 6 cut(s) 19, 530, 610, 656, 702, 860
PkrI GCNGC 9 cut(s) 56, 113, 116, 301, 580, 645, 775, 991, 1047
PleI GAGTC 3 cut(s) 71, 80, 250
PluTI GGCGCC 2 cut(s) 99, 1051
PpsI GAGTC 3 cut(s) 71, 80, 250
PpuMI RGGWCCY 3 cut(s) 217, 258, 504
PshAI GACNNNNGTC 1 cut(s) 263
Psp5II RGGWCCY 3 cut(s) 217, 258, 504
Psp6I CCWGG 3 cut(s) 176, 228, 708
PspGI CCWGG 3 cut(s) 176, 228, 708
PspN4I GGNNCC 8 cut(s) 97, 155, 250, 259, 505, 506, 765, 1049
PspPI GGNCC 7 cut(s) 217, 226, 248, 258, 290, 421, 504
PspPPI RGGWCCY 3 cut(s) 217, 258, 504
PspXI VCTCGAGB 1 cut(s) 221
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 2 cut(s) 719, 1018
RsaI GTAC 3 cut(s) 173, 445, 629
RsaNI GTAC 3 cut(s) 172, 444, 628
RseI CAYNNNNRTG 5 cut(s) 197, 651, 855, 942, 1056
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 4 cut(s) 104, 674, 756, 969
SatI GCNGC 9 cut(s) 55, 112, 115, 300, 579, 644, 774, 990, 1046
Sau3AI GATC 4 cut(s) 237, 719, 1018, 1153
Sau96I GGNCC 7 cut(s) 217, 226, 248, 258, 290, 421, 504
ScaI AGTACT 1 cut(s) 445
SchI GAGTC 3 cut(s) 71, 81, 251
ScrFI CCNGG 4 cut(s) 178, 230, 293, 710
SfaNI GCATC 2 cut(s) 859, 946
SfcI CTRYAG 3 cut(s) 372, 465, 723
SfiI GGCCNNNNNGGCC 1 cut(s) 288
SfoI GGCGCC 2 cut(s) 97, 1049
Sfr274I CTCGAG 1 cut(s) 221
SinI GGWCC 4 cut(s) 217, 226, 258, 504
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 5 cut(s) 197, 651, 855, 942, 1056
SmlI CTYRAG 2 cut(s) 221, 901
SmoI CTYRAG 2 cut(s) 221, 901
Sse9I AATT 6 cut(s) 87, 100, 454, 663, 768, 779
SsiI CCGC 7 cut(s) 54, 111, 114, 117, 300, 350, 1045
SspDI GGCGCC 2 cut(s) 95, 1047
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 176, 228, 291, 708
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 5 cut(s) 62, 287, 466, 753, 1072
TaiI ACGT 1 cut(s) 312
TaqI TCGA 4 cut(s) 75, 222, 240, 615
TasI AATT 6 cut(s) 87, 100, 454, 663, 768, 779
TatI WGTACW 1 cut(s) 443
TauI GCSGC 5 cut(s) 57, 114, 117, 302, 1048
TfiI GAWTC 6 cut(s) 19, 530, 610, 656, 702, 860
Tru1I TTAA 4 cut(s) 104, 674, 756, 969
Tru9I TTAA 4 cut(s) 104, 674, 756, 969
TscAI CASTG 2 cut(s) 571, 601
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 4 cut(s) 578, 643, 773, 989
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 5 cut(s) 32, 755, 815, 924, 1046
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 2 cut(s) 571, 601
VpaK11BI GGWCC 4 cut(s) 217, 226, 258, 504
XagI CCTNNNNNAGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 779
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 3 cut(s) 477, 749, 808
XhoI CTCGAG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 717
ZrmI AGTACT 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.