Rw4G022600

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008465
Physical Location & Seq
Forward (+)
48055293 .. 48058328
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022600.1

Sequence Viewer

Length: 1164 bp
ATGCTTCATGCATTGATGAATCTTCTCTCGCTGTGTTGGAGGCCATTTGGGCGCGGCGACGGTGAGGGAGGAGTCGACTCTATCGGAATTGTTGGCGCCAATTTAAACGGCGGCGGCGGCGGTGGAGGAAGAGATGGCAAAGATAGCTTGCTTTGGTTCCGAGATATCGGAAAGTACGCCTGGGGAGATTTCTCCATGGCTGTGGTTCAGGCCAATCAGGTCCTCGAGGACCAGGGGCAGATCGAGTCCGGCCCCTTTGGGACCTTCGTCGGCGTCTATGATGGCCACGGTGGCCCGGAGGCCGCTCAATACGTCTGCGATAATCTATTTAGACATTTTCAAGCAGTATCAGCGGAGACGGAGGATGTTGTGACAGCAGAGACCATTCAAAATGCTTTTCGTCAGACAGAGGAAGGATTTACGGCCCTTGTTTCTGAGTTGTGGAGTACTAAACCCAATTTAGCAACTGTAGGAACATGCTGTCTGGTTGGGGTGATATGTCAGGGGACCCTATTTGTGGCAAGTCTTGGAGATTCCCGTCTTGTTTTGGGGAAGAAAGTGGGCAACACTGGGGAAGTGGCTGCTATTCAGTTATCCACCGAACACAATGCGAATCTCGATGAGATTAGGTACGAGCTAAAAGAGCTGCACCCACATGATTCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGAATCATCCAGGTTTCTAGATCTATAGGTGATGTATATATGAAACATGCACAGTTTAACAGGGAGCCAATTGCTGCAAAATTCCGACTTCCTGAACCAATGAACATGCCTATCATGTCTGCCACTCCAACTATTCTTTCTCATGCTCTGCATCCAAATGATTCTTTTCTTATCTTTGCGTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAGATTGTCCACAAGCATCCTCATGCAGGAAGTGCCAAAAGGCTTGTTAAGACAGCGCTCCAAGAAGCTGCAAGAAAACGAGAAATGCGTTATTCAGATCTTCGGAAAATAGACAAGAAAGTGCGGCGCCATTTTCATGATGATATAACTGTTATTGTTTTATTCTTGAACCATGACCTAATCTCCAGAGGCACAGTGCAAGAACCCCCAGTCTCAATAAGAAGTGGTCTTGATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

387

Amino Acids

42.51

Weight (kDa)

6.22

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 88 - 333 4.3e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 95, 1050
AccBSI CCGCTC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 54
AciI CCGC 8 cut(s) 54, 111, 114, 117, 120, 303, 353, 1048
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 782
AcyI GRCGYC 3 cut(s) 96, 273, 1051
AfaI GTAC 3 cut(s) 176, 448, 632
AfeI AGCGCT 1 cut(s) 981
AfiI CCNNNNNNNGG 2 cut(s) 517, 950
AgsI TTSAA 3 cut(s) 341, 389, 1093
AjnI CCWGG 3 cut(s) 179, 231, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AluBI AGCT 5 cut(s) 147, 637, 646, 920, 992
AluI AGCT 5 cut(s) 147, 637, 646, 920, 992
Alw26I GTCTC 3 cut(s) 350, 374, 1141
Ama87I CYCGRG 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 981
AoxI GGCC 7 cut(s) 41, 210, 250, 283, 292, 300, 423
ApeKI GCWGC 4 cut(s) 581, 646, 776, 992
ApoI RAATTY 1 cut(s) 782
AspLEI GCGC 4 cut(s) 54, 98, 982, 1053
AspS9I GGNCC 7 cut(s) 220, 229, 251, 261, 293, 424, 507
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 74, 505, 743
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 4 cut(s) 220, 229, 261, 507
BalI TGGCCA 1 cut(s) 285
BanI GGYRCC 2 cut(s) 95, 1050
BbvI GCAGC 4 cut(s) 568, 633, 763, 979
BccI CCATC 3 cut(s) 128, 275, 881
BceAI ACGGC 2 cut(s) 124, 438
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 181, 233, 713
BclI TGATCA 1 cut(s) 1156
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 350, 374, 1141
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 2 cut(s) 468, 726
BfoI RGCGCY 3 cut(s) 99, 983, 1054
BglI GCCNNNNNGGC 2 cut(s) 49, 291
BglII AGATCT 2 cut(s) 722, 1021
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 4 cut(s) 181, 233, 296, 713
Bme18I GGWCC 4 cut(s) 220, 229, 261, 507
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 7 cut(s) 220, 229, 251, 261, 293, 424, 507
BmiI GGNNCC 8 cut(s) 97, 158, 253, 262, 508, 509, 768, 1052
BmrFI CCNGG 4 cut(s) 181, 233, 296, 713
BmrI ACTGGG 2 cut(s) 579, 1127
BmsI GCATC 2 cut(s) 862, 949
BmuI ACTGGG 2 cut(s) 579, 1127
BoxI GACNNNNGTC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 1093
BpuEI CTTGAG 1 cut(s) 925
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 3 cut(s) 96, 273, 1051
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 180, 195, 232, 286
BsaXI ACNNNNNCTCC 2 cut(s) 1091, 1121
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 950
Bse1I ACTGG 2 cut(s) 574, 1133
BseBI CCWGG 3 cut(s) 181, 233, 713
BseDI CCNNGG 4 cut(s) 180, 195, 232, 286
BseGI GGATG 4 cut(s) 370, 708, 853, 940
BseLI CCNNNNNNNGG 2 cut(s) 517, 950
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 574, 1133
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 4 cut(s) 568, 633, 763, 979
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 7 cut(s) 43, 212, 252, 285, 294, 302, 425
BshNI GGYRCC 2 cut(s) 95, 1050
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 2 cut(s) 249, 296
BslFI GGGAC 2 cut(s) 274, 520
BslI CCNNNNNNNGG 2 cut(s) 517, 950
BsmAI GTCTC 3 cut(s) 350, 374, 1141
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 274, 520
BsnI GGCC 7 cut(s) 43, 212, 252, 285, 294, 302, 425
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 224
Bsp143I GATC 4 cut(s) 240, 722, 1021, 1156
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 8 cut(s) 54, 111, 114, 117, 120, 303, 353, 1048
BspANI GGCC 7 cut(s) 43, 212, 252, 285, 294, 302, 425
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 8 cut(s) 97, 158, 253, 262, 508, 509, 768, 1052
BspT107I GGYRCC 2 cut(s) 95, 1050
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 305
BsrI ACTGG 2 cut(s) 574, 1133
BssECI CCNNGG 4 cut(s) 180, 195, 232, 286
BssMI GATC 4 cut(s) 240, 722, 1021, 1156
BssNI GRCGYC 3 cut(s) 96, 273, 1051
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 181, 233, 713
Bst4CI ACNGT 6 cut(s) 62, 290, 469, 756, 1075, 1120
Bst6I CTCTTC 1 cut(s) 124
BstACI GRCGYC 3 cut(s) 96, 273, 1051
BstAPI GCANNNNNTGC 1 cut(s) 956
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 2 cut(s) 195, 286
BstENI CCTNNNNNAGG 1 cut(s) 948
BstF5I GGATG 4 cut(s) 370, 708, 853, 940
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 3 cut(s) 99, 983, 1054
BstHHI GCGC 4 cut(s) 54, 98, 982, 1053
BstKTI GATC 4 cut(s) 243, 725, 1024, 1159
BstMAI GTCTC 3 cut(s) 350, 374, 1141
BstMBI GATC 4 cut(s) 240, 722, 1021, 1156
BstMWI GCNNNNNNNGC 7 cut(s) 49, 117, 144, 291, 350, 643, 956
BstNI CCWGG 3 cut(s) 181, 233, 713
BstNSI RCATGY 3 cut(s) 480, 752, 811
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 179, 231, 294, 711
BstSFI CTRYAG 2 cut(s) 468, 726
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 4 cut(s) 568, 633, 763, 979
BstX2I RGATCY 2 cut(s) 722, 1021
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 2 cut(s) 722, 1021
BsuRI GGCC 7 cut(s) 43, 212, 252, 285, 294, 302, 425
BtgI CCRYGG 2 cut(s) 195, 286
BtsCI GGATG 4 cut(s) 370, 708, 853, 940
BtsIMutI CAGTG 2 cut(s) 567, 1125
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 4 cut(s) 54, 98, 982, 1053
Cfr13I GGNCC 7 cut(s) 220, 229, 251, 261, 293, 424, 507
CseI GACGC 2 cut(s) 262, 870
Csp6I GTAC 3 cut(s) 175, 447, 631
CviQI GTAC 3 cut(s) 175, 447, 631
DdeI CTNAG 1 cut(s) 435
DinI GGCGCC 2 cut(s) 97, 1052
DpnI GATC 4 cut(s) 242, 724, 1023, 1158
DpnII GATC 4 cut(s) 240, 722, 1021, 1156
DraI TTTAAA 2 cut(s) 105, 678
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 374
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 4 cut(s) 220, 229, 261, 507
Eco47III AGCGCT 1 cut(s) 981
Eco88I CYCGRG 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 948
EcoO109I RGGNCCY 3 cut(s) 220, 261, 507
EcoRII CCWGG 3 cut(s) 179, 231, 711
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 13
EgeI GGCGCC 2 cut(s) 97, 1052
EheI GGCGCC 2 cut(s) 97, 1052
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 1 cut(s) 350
FaqI GGGAC 2 cut(s) 274, 520
FbaI TGATCA 1 cut(s) 1156
FblI GTMKAC 1 cut(s) 75
FokI GGATG 4 cut(s) 377, 695, 840, 927
FspBI CTAG 1 cut(s) 720
GlaI GCGC 4 cut(s) 53, 97, 981, 1052
GsuI CTGGAG 1 cut(s) 1093
HaeII RGCGCY 3 cut(s) 99, 983, 1054
HaeIII GGCC 7 cut(s) 43, 212, 252, 285, 294, 302, 425
HapII CCGG 2 cut(s) 249, 296
HgaI GACGC 2 cut(s) 262, 870
HhaI GCGC 4 cut(s) 54, 98, 982, 1053
Hin1I GRCGYC 3 cut(s) 96, 273, 1051
Hin6I GCGC 4 cut(s) 52, 96, 980, 1051
HinP1I GCGC 4 cut(s) 52, 96, 980, 1051
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 9 cut(s) 19, 72, 77, 245, 533, 613, 659, 705, 863
HpaII CCGG 2 cut(s) 249, 296
HphI GGTGA 3 cut(s) 74, 505, 743
Hpy166II GTNNAC 2 cut(s) 76, 934
Hpy188I TCNGA 9 cut(s) 86, 161, 170, 405, 436, 788, 886, 1021, 1029
Hpy188III TCNNGA 7 cut(s) 617, 720, 794, 1061, 1090, 1110, 1154
Hpy8I GTNNAC 2 cut(s) 76, 934
Hpy99I CGWCG 2 cut(s) 62, 272
HpyAV CCTTC 2 cut(s) 274, 407
HpyCH4III ACNGT 6 cut(s) 62, 290, 469, 756, 1075, 1120
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 8 cut(s) 11, 649, 752, 779, 853, 950, 995, 1123
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 117, 144, 291, 350, 643, 956
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 3 cut(s) 96, 273, 1051
HspAI GCGC 4 cut(s) 52, 96, 980, 1051
KasI GGCGCC 2 cut(s) 95, 1050
KflI GGGWCCC 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 1156
Kzo9I GATC 4 cut(s) 240, 722, 1021, 1156
LmnI GCTCC 2 cut(s) 766, 987
Lsp1109I GCAGC 4 cut(s) 568, 633, 763, 979
LweI GCATC 2 cut(s) 862, 949
MaeI CTAG 1 cut(s) 720
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 370
MalI GATC 4 cut(s) 242, 724, 1023, 1158
MbiI CCGCTC 1 cut(s) 305
MboI GATC 4 cut(s) 240, 722, 1021, 1156
MboII GAAGA 4 cut(s) 14, 141, 565, 1016
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 722, 1021
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 6 cut(s) 87, 100, 457, 666, 771, 782
MluNI TGGCCA 1 cut(s) 285
Mly113I GGCGCC 2 cut(s) 96, 1051
MlyI GAGTC 3 cut(s) 71, 81, 254
MmeI TCCRAC 3 cut(s) 17, 811, 854
Mox20I TGGCCA 1 cut(s) 285
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 1 cut(s) 285
MseI TTAA 4 cut(s) 104, 677, 759, 972
MslI CAYNNNNRTG 5 cut(s) 200, 654, 858, 945, 1059
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 249, 296
MspR9I CCNGG 4 cut(s) 181, 233, 296, 713
MunI CAATTG 1 cut(s) 771
MvaI CCWGG 3 cut(s) 181, 233, 713
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 7 cut(s) 49, 117, 144, 291, 350, 643, 956
NarI GGCGCC 2 cut(s) 96, 1051
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 195
NdeII GATC 4 cut(s) 240, 722, 1021, 1156
NlaIV GGNNCC 8 cut(s) 97, 158, 253, 262, 508, 509, 768, 1052
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 480, 752, 811
PaeR7I CTCGAG 1 cut(s) 224
PagI TCATGA 1 cut(s) 1060
PcsI WCGNNNNNNNCGW 1 cut(s) 1009
PfeI GAWTC 6 cut(s) 19, 533, 613, 659, 705, 863
PleI GAGTC 3 cut(s) 71, 80, 253
PluTI GGCGCC 2 cut(s) 99, 1054
PpsI GAGTC 3 cut(s) 71, 80, 253
PpuMI RGGWCCY 3 cut(s) 220, 261, 507
PshAI GACNNNNGTC 1 cut(s) 266
Psp5II RGGWCCY 3 cut(s) 220, 261, 507
Psp6I CCWGG 3 cut(s) 179, 231, 711
PspGI CCWGG 3 cut(s) 179, 231, 711
PspN4I GGNNCC 8 cut(s) 97, 158, 253, 262, 508, 509, 768, 1052
PspPI GGNCC 7 cut(s) 220, 229, 251, 261, 293, 424, 507
PspPPI RGGWCCY 3 cut(s) 220, 261, 507
PspXI VCTCGAGB 1 cut(s) 224
PsuI RGATCY 2 cut(s) 722, 1021
RsaI GTAC 3 cut(s) 176, 448, 632
RsaNI GTAC 3 cut(s) 175, 447, 631
RseI CAYNNNNRTG 5 cut(s) 200, 654, 858, 945, 1059
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 4 cut(s) 104, 677, 759, 972
Sau3AI GATC 4 cut(s) 240, 722, 1021, 1156
Sau96I GGNCC 7 cut(s) 220, 229, 251, 261, 293, 424, 507
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 3 cut(s) 71, 81, 254
ScrFI CCNGG 4 cut(s) 181, 233, 296, 713
SfaNI GCATC 2 cut(s) 862, 949
SfcI CTRYAG 2 cut(s) 468, 726
SfiI GGCCNNNNNGGCC 1 cut(s) 291
SfoI GGCGCC 2 cut(s) 97, 1052
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 4 cut(s) 220, 229, 261, 507
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 5 cut(s) 200, 654, 858, 945, 1059
SmlI CTYRAG 2 cut(s) 224, 904
SmoI CTYRAG 2 cut(s) 224, 904
Sse9I AATT 6 cut(s) 87, 100, 457, 666, 771, 782
SsiI CCGC 8 cut(s) 54, 111, 114, 117, 120, 303, 353, 1048
SspDI GGCGCC 2 cut(s) 95, 1050
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 4 cut(s) 179, 231, 294, 711
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 6 cut(s) 62, 290, 469, 756, 1075, 1120
TaiI ACGT 1 cut(s) 315
TaqI TCGA 4 cut(s) 75, 225, 243, 618
TasI AATT 6 cut(s) 87, 100, 457, 666, 771, 782
TatI WGTACW 1 cut(s) 446
TauI GCSGC 6 cut(s) 57, 114, 117, 120, 305, 1051
TfiI GAWTC 6 cut(s) 19, 533, 613, 659, 705, 863
Tru1I TTAA 4 cut(s) 104, 677, 759, 972
Tru9I TTAA 4 cut(s) 104, 677, 759, 972
TscAI CASTG 2 cut(s) 574, 1125
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 4 cut(s) 581, 646, 776, 992
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 5 cut(s) 32, 758, 818, 927, 1049
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 2 cut(s) 574, 1125
VpaK11BI GGWCC 4 cut(s) 220, 229, 261, 507
XagI CCTNNNNNAGG 1 cut(s) 948
XapI RAATTY 1 cut(s) 782
XbaI TCTAGA 1 cut(s) 719
XceI RCATGY 3 cut(s) 480, 752, 811
XhoI CTCGAG 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 720
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.