Rroxscaffold_5G00367530

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008465
Physical Location & Seq
Forward (+)
49343900 .. 49347827
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367530.1

Sequence Viewer

Length: 867 bp
ATGAGCTGCTCTTATGTTGGCTATCTTAGGCATTGTGTTTATGCAGCAGTATCAGCGGAGACGGAGGATGTTGTGACAGCAGAGACCATTCAAAATGCTTTTCGTCAGACAGAGGAAGGATTTACGGCCCTTGTTTCTGAGTTGTGGAGTACTAAACCCAATTTAGCAACTGTAGGAACATGCTGTCTGGTTGGGGTGATATGTCAGGGGACCCTATTTGTGGCAAGTCTTGGAGATTCCCGTCTTGTTTTGGGGAAGAAAGTGGGCAACACTGGGGAAGTTGCTGCTATTCAGTTATCCACCGAACACAATGCGAATCTCGATGAGATTAGGTACGAGCTAAAAGAGCTGCACCCACATGATTCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGAATCATCCAGGTTTCTAGATCTATAGGTGATGTATATATGAAACATGCACAGTTTAACAGGGAGCCAATTGCTGCAAAATTCCGACTTCCTGAACCAATGAACATGCCTATCATGTCTGCCACTCCAACTATTCTTTCTCATGCTCTGCATCCAAATGATTCTTTTCTTATCTTTGCGTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAGATTGTCCACAAGCATCCTCATGCAGGAAGTGCCAAAAGGCTTGTTAAGACAGCGCTCCAAGAAGCTGCAAGAAAACGAGAAATGCGTTATTCAGATCTTCGGAAAATAGACAAGAAAGTGCGGCGCCATTTTCATGATGATATAACTGTTATTGTTTTATTCTTGAACCATGACCTAATCTCCAGAGGCACAGCGCAAGAACCTCCAGTCTCAATTAGAAGTGGCCTTGATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

288

Amino Acids

32.13

Weight (kDa)

7.28

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 40 - 234 2.4e-29 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 753
AciI CCGC 2 cut(s) 56, 751
AcsI RAATTY 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 754
AfaI GTAC 2 cut(s) 151, 335
AfeI AGCGCT 1 cut(s) 684
AfiI CCNNNNNNNGG 2 cut(s) 220, 653
AgsI TTSAA 2 cut(s) 92, 796
AjnI CCWGG 1 cut(s) 414
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AluBI AGCT 5 cut(s) 6, 340, 349, 623, 695
AluI AGCT 5 cut(s) 6, 340, 349, 623, 695
Alw26I GTCTC 3 cut(s) 53, 77, 844
Aor51HI AGCGCT 1 cut(s) 684
AoxI GGCC 2 cut(s) 126, 853
ApeKI GCWGC 6 cut(s) 6, 44, 284, 349, 479, 695
ApoI RAATTY 1 cut(s) 485
AspLEI GCGC 3 cut(s) 685, 756, 826
AspS9I GGNCC 2 cut(s) 127, 210
AsuHPI GGTGA 2 cut(s) 208, 446
AvaII GGWCC 1 cut(s) 210
BanI GGYRCC 1 cut(s) 753
BbvI GCAGC 5 cut(s) 56, 271, 336, 466, 682
BccI CCATC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 141
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 416
BclI TGATCA 1 cut(s) 859
BcoDI GTCTC 3 cut(s) 53, 77, 844
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 2 cut(s) 171, 429
BfoI RGCGCY 2 cut(s) 686, 757
BglII AGATCT 2 cut(s) 425, 724
BisI GCNGC 7 cut(s) 7, 45, 285, 350, 480, 696, 752
BlsI GCNGC 7 cut(s) 8, 46, 286, 351, 481, 697, 753
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 127, 210
BmiI GGNNCC 4 cut(s) 211, 212, 471, 755
BmrFI CCNGG 1 cut(s) 416
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 565, 652
BmuI ACTGGG 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 796, 819
BpuEI CTTGAG 1 cut(s) 628
BsaHI GRCGYC 1 cut(s) 754
BsaI GGTCTC 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 653
Bse1I ACTGG 2 cut(s) 277, 836
BseBI CCWGG 1 cut(s) 416
BseGI GGATG 4 cut(s) 73, 411, 556, 643
BseLI CCNNNNNNNGG 2 cut(s) 220, 653
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 277, 836
BseXI GCAGC 5 cut(s) 56, 271, 336, 466, 682
BsgI GTGCAG 1 cut(s) 335
BshFI GGCC 2 cut(s) 128, 855
BshNI GGYRCC 1 cut(s) 753
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 220, 653
BsmAI GTCTC 3 cut(s) 53, 77, 844
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 2 cut(s) 128, 855
Bso31I GGTCTC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 425, 724, 859
BspACI CCGC 2 cut(s) 56, 751
BspANI GGCC 2 cut(s) 128, 855
BspCNI CTCAG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 4 cut(s) 211, 212, 471, 755
BspT107I GGYRCC 1 cut(s) 753
BspTNI GGTCTC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 277, 836
BssMI GATC 3 cut(s) 425, 724, 859
BssNI GRCGYC 1 cut(s) 754
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 3 cut(s) 172, 459, 778
BstACI GRCGYC 1 cut(s) 754
BstAPI GCANNNNNTGC 1 cut(s) 659
BstDEI CTNAG 2 cut(s) 26, 138
BstENI CCTNNNNNAGG 1 cut(s) 651
BstF5I GGATG 4 cut(s) 73, 411, 556, 643
BstH2I RGCGCY 2 cut(s) 686, 757
BstHHI GCGC 3 cut(s) 685, 756, 826
BstKTI GATC 3 cut(s) 428, 727, 862
BstMAI GTCTC 3 cut(s) 53, 77, 844
BstMBI GATC 3 cut(s) 425, 724, 859
BstMWI GCNNNNNNNGC 3 cut(s) 53, 346, 659
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 3 cut(s) 183, 455, 514
BstSCI CCNGG 1 cut(s) 414
BstSFI CTRYAG 2 cut(s) 171, 429
BstV1I GCAGC 5 cut(s) 56, 271, 336, 466, 682
BstX2I RGATCY 2 cut(s) 425, 724
BstYI RGATCY 2 cut(s) 425, 724
BsuRI GGCC 2 cut(s) 128, 855
BtsCI GGATG 4 cut(s) 73, 411, 556, 643
BtsIMutI CAGTG 1 cut(s) 270
CciI TCATGA 1 cut(s) 763
CfoI GCGC 3 cut(s) 685, 756, 826
Cfr13I GGNCC 2 cut(s) 127, 210
CseI GACGC 1 cut(s) 573
Csp6I GTAC 2 cut(s) 150, 334
CviQI GTAC 2 cut(s) 150, 334
DdeI CTNAG 2 cut(s) 26, 138
DinI GGCGCC 1 cut(s) 755
DpnI GATC 3 cut(s) 427, 726, 861
DpnII GATC 3 cut(s) 425, 724, 859
DraI TTTAAA 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 210
Eco47III AGCGCT 1 cut(s) 684
EcoNI CCTNNNNNAGG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 755
EheI GGCGCC 1 cut(s) 755
Esp3I CGTCTC 1 cut(s) 53
FaqI GGGAC 1 cut(s) 223
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 7 cut(s) 7, 45, 285, 350, 480, 696, 752
FokI GGATG 4 cut(s) 80, 398, 543, 630
Fsp4HI GCNGC 7 cut(s) 7, 45, 285, 350, 480, 696, 752
FspBI CTAG 1 cut(s) 423
GlaI GCGC 3 cut(s) 684, 755, 825
GluI GCNGC 7 cut(s) 7, 45, 285, 350, 480, 696, 752
GsuI CTGGAG 2 cut(s) 796, 819
HaeII RGCGCY 2 cut(s) 686, 757
HaeIII GGCC 2 cut(s) 128, 855
HgaI GACGC 1 cut(s) 573
HhaI GCGC 3 cut(s) 685, 756, 826
Hin1I GRCGYC 1 cut(s) 754
Hin6I GCGC 3 cut(s) 683, 754, 824
HinP1I GCGC 3 cut(s) 683, 754, 824
HinfI GANTC 5 cut(s) 236, 316, 362, 408, 566
HphI GGTGA 2 cut(s) 208, 446
Hpy166II GTNNAC 1 cut(s) 637
Hpy188I TCNGA 6 cut(s) 108, 139, 491, 589, 724, 732
Hpy188III TCNNGA 6 cut(s) 320, 423, 497, 764, 793, 813
Hpy8I GTNNAC 1 cut(s) 637
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 3 cut(s) 172, 459, 778
HpyCH4V TGCA 7 cut(s) 44, 352, 455, 482, 556, 653, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 346, 659
HpyF3I CTNAG 2 cut(s) 26, 138
Hsp92I GRCGYC 1 cut(s) 754
HspAI GCGC 3 cut(s) 683, 754, 824
KasI GGCGCC 1 cut(s) 753
KflI GGGWCCC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 859
Kzo9I GATC 3 cut(s) 425, 724, 859
LmnI GCTCC 2 cut(s) 469, 690
Lsp1109I GCAGC 5 cut(s) 56, 271, 336, 466, 682
LweI GCATC 2 cut(s) 565, 652
MaeI CTAG 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 73
MalI GATC 3 cut(s) 427, 726, 861
MboI GATC 3 cut(s) 425, 724, 859
MboII GAAGA 2 cut(s) 268, 719
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 2 cut(s) 425, 724
MluCI AATT 5 cut(s) 160, 369, 474, 485, 843
Mly113I GGCGCC 1 cut(s) 754
MmeI TCCRAC 2 cut(s) 514, 557
MnlI CCTC 5 cut(s) 58, 106, 657, 809, 843
MseI TTAA 3 cut(s) 380, 462, 675
MslI CAYNNNNRTG 4 cut(s) 357, 561, 648, 762
MspA1I CMGCKG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 416
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 3 cut(s) 53, 346, 659
NarI GGCGCC 1 cut(s) 754
NdeII GATC 3 cut(s) 425, 724, 859
NlaIV GGNNCC 4 cut(s) 211, 212, 471, 755
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 3 cut(s) 183, 455, 514
PagI TCATGA 1 cut(s) 763
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PfeI GAWTC 5 cut(s) 236, 316, 362, 408, 566
PkrI GCNGC 7 cut(s) 8, 46, 286, 351, 481, 697, 753
PluTI GGCGCC 1 cut(s) 757
PpuMI RGGWCCY 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 4 cut(s) 211, 212, 471, 755
PspPI GGNCC 2 cut(s) 127, 210
PspPPI RGGWCCY 1 cut(s) 210
PsuI RGATCY 2 cut(s) 425, 724
RsaI GTAC 2 cut(s) 151, 335
RsaNI GTAC 2 cut(s) 150, 334
RseI CAYNNNNRTG 4 cut(s) 357, 561, 648, 762
SaqAI TTAA 3 cut(s) 380, 462, 675
SatI GCNGC 7 cut(s) 7, 45, 285, 350, 480, 696, 752
Sau3AI GATC 3 cut(s) 425, 724, 859
Sau96I GGNCC 2 cut(s) 127, 210
ScaI AGTACT 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 416
SfaNI GCATC 2 cut(s) 565, 652
SfcI CTRYAG 2 cut(s) 171, 429
SfoI GGCGCC 1 cut(s) 755
SinI GGWCC 1 cut(s) 210
SmiMI CAYNNNNRTG 4 cut(s) 357, 561, 648, 762
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 5 cut(s) 160, 369, 474, 485, 843
SsiI CCGC 2 cut(s) 56, 751
SspDI GGCGCC 1 cut(s) 753
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 1 cut(s) 414
TaaI ACNGT 3 cut(s) 172, 459, 778
TaqI TCGA 1 cut(s) 321
TasI AATT 5 cut(s) 160, 369, 474, 485, 843
TatI WGTACW 1 cut(s) 149
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 5 cut(s) 236, 316, 362, 408, 566
Tru1I TTAA 3 cut(s) 380, 462, 675
Tru9I TTAA 3 cut(s) 380, 462, 675
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 6 cut(s) 6, 44, 284, 349, 479, 695
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 4 cut(s) 461, 521, 630, 752
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 210
XagI CCTNNNNNAGG 1 cut(s) 651
XapI RAATTY 1 cut(s) 485
XbaI TCTAGA 1 cut(s) 422
XceI RCATGY 3 cut(s) 183, 455, 514
XspI CTAG 1 cut(s) 423
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.