pycom13g13000

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008465
Physical Location & Seq
Reverse (-)
9219658 .. 9220748
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g13000.1

Sequence Viewer

Length: 660 bp
ATGCTTCAGGCATTGATGAATCTGCTCTCTCTTTGCTGGAAGCCATTTGGGCGCAGCGACGGTGAGGGACTTGGCTCTGTCGGAATCGTCGGCGCTGCGAACATCGGAGGCGGCGGTGGAGGACGAGATGGCAAAGATAATTTGCTTTGGTTCCGAGATTTCGGAAAGTACGCGTGGGGCGATTTCTCCATGGCGGTGGTCCAAGCCAATCAGGTGCTCGAGGACCAGAGCCAGATCGAGTCTGGACCCTTTGGAACCTTCGTCGGCGTATACGACGGACATGGCGGCCCGGAGACCGCTCGATACGTTTGCGACAATTTATTTAGACATTTCCAAGCAATATCAGCGGAGTCTGAAGGTGTTGTGACAGCAGAGACTATCCAAAATGCTTTTCTCCGAACAGAGGAAGGGTTTACTGCCCTTGTTTCGGAGCTGTGGGGTACTAGACCCCAAGTAGCAACTGTCGGGTCATGCTGTCTGGTTGGAGTAATATGTCAGGGGACCCTATTTGTGGCAAACTTGGGAGATTCACGGTGTGTGTTGGGGAAGAAAGTGGGCAACACTGGGGAAATTGCTGCGATCCAGTTGTCCACTGAACACAATGCAAATCTCGAGGAGGTTAGGCATGAGCTGAAAGATTTGCACCCACATCGATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

220

Amino Acids

23.42

Weight (kDa)

5.09

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 205 2.4e-12 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 173
AciI CCGC 6 cut(s) 111, 114, 194, 285, 297, 347
AclWI GGATC 2 cut(s) 574, 648
AcuI CTGAAG 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 2 cut(s) 170, 442
AfiI CCNNNNNNNGG 3 cut(s) 403, 427, 511
AflIII ACRYGT 1 cut(s) 171
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AluBI AGCT 2 cut(s) 433, 631
AluI AGCT 2 cut(s) 433, 631
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 287, 368
AlwI GGATC 2 cut(s) 574, 648
Ama87I CYCGRG 2 cut(s) 218, 611
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 3 cut(s) 54, 95, 575
AspLEI GCGC 2 cut(s) 54, 95
AspS9I GGNCC 5 cut(s) 199, 223, 245, 287, 501
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 218, 611
AvaII GGWCC 4 cut(s) 199, 223, 245, 501
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 66, 82, 562
BccI CCATC 1 cut(s) 122
BcgI CGANNNNNNTGC 3 cut(s) 291, 325, 632
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 287, 368
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 96
BglI GCCNNNNNGGC 1 cut(s) 49
BisI GCNGC 5 cut(s) 55, 96, 112, 286, 576
BlsI GCNGC 5 cut(s) 56, 97, 113, 287, 577
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 4 cut(s) 199, 223, 245, 501
BmeT110I CYCGRG 2 cut(s) 218, 611
BmgT120I GGNCC 5 cut(s) 199, 223, 245, 287, 501
BmiI GGNNCC 5 cut(s) 152, 247, 256, 502, 503
BmrFI CCNGG 1 cut(s) 290
BmrI ACTGGG 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 290
Bsa29I ATCGAT 1 cut(s) 652
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 427, 511
Bse1I ACTGG 2 cut(s) 568, 583
BseCI ATCGAT 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 189
BseLI CCNNNNNNNGG 3 cut(s) 403, 427, 511
BseNI ACTGG 2 cut(s) 568, 583
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 3 cut(s) 66, 82, 562
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 288
BshVI ATCGAT 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 218, 611
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 2 cut(s) 81, 514
BslI CCNNNNNNNGG 3 cut(s) 403, 427, 511
BsmAI GTCTC 2 cut(s) 287, 368
BsmFI GGGAC 2 cut(s) 81, 514
BsnI GGCC 1 cut(s) 288
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 218, 611
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 3 cut(s) 234, 579, 653
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 6 cut(s) 111, 114, 194, 285, 297, 347
BspANI GGCC 1 cut(s) 288
BspDI ATCGAT 1 cut(s) 652
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 5 cut(s) 152, 247, 256, 502, 503
BspPI GGATC 2 cut(s) 574, 648
BspTNI GGTCTC 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 2 cut(s) 568, 583
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 3 cut(s) 234, 579, 653
BssNAI GTATAC 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 271
Bst4CI ACNGT 3 cut(s) 62, 463, 534
BstDSI CCRYGG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 54, 95
BstKTI GATC 3 cut(s) 237, 582, 656
BstMAI GTCTC 2 cut(s) 287, 368
BstMBI GATC 3 cut(s) 234, 579, 653
BstMWI GCNNNNNNNGC 2 cut(s) 49, 344
BstSCI CCNGG 1 cut(s) 288
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 66, 82, 562
BstXI CCANNNNNNTGG 1 cut(s) 196
BstZ17I GTATAC 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 652
BsuRI GGCC 1 cut(s) 288
BsuTUI ATCGAT 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 561, 591
CfoI GCGC 2 cut(s) 54, 95
Cfr13I GGNCC 5 cut(s) 199, 223, 245, 287, 501
ClaI ATCGAT 1 cut(s) 652
Csp6I GTAC 2 cut(s) 169, 441
CviAII CATG 4 cut(s) 190, 281, 471, 626
CviJI RGCY 7 cut(s) 43, 75, 206, 231, 288, 433, 631
CviKI_1 RGCY 7 cut(s) 43, 75, 206, 231, 288, 433, 631
CviQI GTAC 2 cut(s) 169, 441
DpnI GATC 3 cut(s) 236, 581, 655
DpnII GATC 3 cut(s) 234, 579, 653
DraIII CACNNNGTG 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 4 cut(s) 199, 223, 245, 501
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 2 cut(s) 218, 611
EcoO109I RGGNCCY 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 4 cut(s) 193, 284, 474, 629
FaiI YATR 6 cut(s) 191, 271, 282, 472, 493, 627
FaqI GGGAC 2 cut(s) 81, 514
FatI CATG 4 cut(s) 189, 280, 470, 625
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 55, 96, 112, 286, 576
Fsp4HI GCNGC 5 cut(s) 55, 96, 112, 286, 576
FspBI CTAG 1 cut(s) 444
GlaI GCGC 2 cut(s) 53, 94
GluI GCNGC 5 cut(s) 55, 96, 112, 286, 576
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 288
HapII CCGG 1 cut(s) 290
HhaI GCGC 2 cut(s) 54, 95
Hin1II CATG 4 cut(s) 193, 284, 474, 629
Hin6I GCGC 2 cut(s) 52, 93
HinP1I GCGC 2 cut(s) 52, 93
HinfI GANTC 5 cut(s) 19, 84, 239, 350, 527
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 271, 414, 591
Hpy188I TCNGA 7 cut(s) 83, 107, 155, 164, 355, 398, 430
Hpy188III TCNNGA 3 cut(s) 243, 611, 657
Hpy8I GTNNAC 3 cut(s) 271, 414, 591
Hpy99I CGWCG 4 cut(s) 62, 92, 266, 278
HpyAV CCTTC 3 cut(s) 268, 350, 401
HpyCH4III ACNGT 3 cut(s) 62, 463, 534
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 2 cut(s) 605, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 344
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 4 cut(s) 193, 284, 474, 629
HspAI GCGC 2 cut(s) 52, 93
KflI GGGWCCC 1 cut(s) 501
Kzo9I GATC 3 cut(s) 234, 579, 653
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 3 cut(s) 66, 82, 562
MaeI CTAG 1 cut(s) 444
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 364
MalI GATC 3 cut(s) 236, 581, 655
MbiI CCGCTC 1 cut(s) 299
MboI GATC 3 cut(s) 234, 579, 653
MboII GAAGA 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 3 cut(s) 139, 316, 570
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 2 cut(s) 248, 359
MmeI TCCRAC 2 cut(s) 61, 463
MnlI CCTC 7 cut(s) 58, 101, 113, 214, 397, 607, 610
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 49, 344
NciI CCSGG 1 cut(s) 290
NcoI CCATGG 1 cut(s) 189
NdeII GATC 3 cut(s) 234, 579, 653
NlaIII CATG 4 cut(s) 193, 284, 474, 629
NlaIV GGNNCC 5 cut(s) 152, 247, 256, 502, 503
NmuCI GTSAC 1 cut(s) 364
PaeR7I CTCGAG 2 cut(s) 218, 611
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 270
PfeI GAWTC 3 cut(s) 19, 84, 527
PkrI GCNGC 5 cut(s) 56, 97, 113, 287, 577
PleI GAGTC 2 cut(s) 247, 358
PpsI GAGTC 2 cut(s) 247, 358
PpuMI RGGWCCY 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 501
PspN4I GGNNCC 5 cut(s) 152, 247, 256, 502, 503
PspPI GGNCC 5 cut(s) 199, 223, 245, 287, 501
PspPPI RGGWCCY 1 cut(s) 501
PspXI VCTCGAGB 1 cut(s) 218
RsaI GTAC 2 cut(s) 170, 442
RsaNI GTAC 2 cut(s) 169, 441
RseI CAYNNNNRTG 1 cut(s) 194
SatI GCNGC 5 cut(s) 55, 96, 112, 286, 576
Sau3AI GATC 3 cut(s) 234, 579, 653
Sau96I GGNCC 5 cut(s) 199, 223, 245, 287, 501
SchI GAGTC 2 cut(s) 248, 359
ScrFI CCNGG 1 cut(s) 290
SduI GDGCHC 1 cut(s) 219
SetI ASST 7 cut(s) 216, 260, 309, 361, 435, 621, 633
Sfr274I CTCGAG 2 cut(s) 218, 611
SinI GGWCC 4 cut(s) 199, 223, 245, 501
SlaI CTCGAG 2 cut(s) 218, 611
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 218, 611
SmoI CTYRAG 2 cut(s) 218, 611
Sse9I AATT 3 cut(s) 139, 316, 570
SsiI CCGC 6 cut(s) 111, 114, 194, 285, 297, 347
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 3 cut(s) 62, 463, 534
TaiI ACGT 1 cut(s) 309
TaqI TCGA 5 cut(s) 219, 237, 301, 612, 652
TasI AATT 3 cut(s) 139, 316, 570
TauI GCSGC 2 cut(s) 114, 288
TfiI GAWTC 3 cut(s) 19, 84, 527
TscAI CASTG 2 cut(s) 568, 598
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 3 cut(s) 54, 95, 575
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 568, 598
VpaK11BI GGWCC 4 cut(s) 199, 223, 245, 501
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 2 cut(s) 218, 611
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.