MD17G1144700.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008465
Physical Location & Seq
Reverse (-)
13045629 .. 13046789
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1144700.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCTTCAGGCATTGATGAATCTGCTCTCTCTTTGCTGGAGTCCATTTGGGCGCGGCGACGGTGAGGGACTCAGCTCTGTTGGAATCGTCGGCGCTGCGAATAACGAAGACGGCGGAGGAGGGCGAGATGGCAAAGATAACTTGCTTTGGTTCCGAGATTTTGGAAAGTACGCGTGGGGCGATTTCTCTATGGCGGTGGTCCAGGCCAATCAGGTGCTCGAGGATCAGAGCCAGATCGAGTCGGGACCCTTTGGGACCTTCGTCGGCGTATACGACGGACACGGCGGACCAGAGACCGCTCGATACGTTTGCGACAATTTATTCAAACATTTCCAAGCAATATCAGCGGAGTCCAAAGGTGTTGTGACAACGGAGACCATTCAAAATGCTTTTCTCCGAACGGATGAAGGGTTTACCGCCCTTGTTTCGAAGCTGTGGGGTACTAGACCCCAAGTAGCAACTGTTGGGTCATGCTGTCTGGTTAGGCATGAACTGAAAGATTTGCACCCACATGATCCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGCATTATCCAGGTATCTAAATCTATAGGTGACGCATATATGAAACATGCAAGGTTTAACAAGGAGCCGGTTAGTGCAAAATTCAGACTTCCCGAACCAATGACCATGCCTACCTTGTCTGCCGAACCAACCATTTTTTCTCATCCTCTCCATCCAAATGATTCTTTTCTTATATTTGCTTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAAATTGTCCACAATCATCCACATGCGGGAAGCGCCAAGCGGCTTGTTAAGACGGCTCTCCAAGAAGCTGCAAGAAAAAGAGAGATGCGATATTCAGATCTTCAGAAAATTGACAAGAAGGTCCGACGTCATTTTCACGATGATATAACTGTCATTGTTTTATTCTTAAACCGTGACCTAATATCCAGAGAGAGAGTGGAAGAGCCCCCAGTCTCAATAAAAAGTGCTCGTGACCAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

341

Amino Acids

37.85

Weight (kDa)

6.74

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 182 - 285 2.1e-17 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 899
AatII GACGTC 1 cut(s) 910
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 54, 173
AciI CCGC 9 cut(s) 54, 114, 194, 285, 297, 347, 417, 806, 820
AclWI GGATC 2 cut(s) 231, 509
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 1 cut(s) 866
AcyI GRCGYC 1 cut(s) 907
AfaI GTAC 2 cut(s) 170, 442
AfiI CCNNNNNNNGG 1 cut(s) 806
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 3 cut(s) 325, 383, 782
AjnI CCWGG 2 cut(s) 201, 567
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AluBI AGCT 4 cut(s) 75, 433, 776, 848
AluI AGCT 4 cut(s) 75, 433, 776, 848
Alw21I GWGCWC 2 cut(s) 219, 1009
Alw26I GTCTC 3 cut(s) 287, 368, 997
AlwI GGATC 2 cut(s) 231, 509
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 95, 848
ApoI RAATTY 1 cut(s) 638
AspLEI GCGC 3 cut(s) 54, 95, 815
AspS9I GGNCC 5 cut(s) 199, 245, 255, 287, 901
AsuHPI GGTGA 2 cut(s) 74, 599
AsuII TTCGAA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 5 cut(s) 199, 245, 255, 287, 901
BanII GRGCYC 1 cut(s) 987
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 219, 1009
BbvI GCAGC 2 cut(s) 82, 835
BccI CCATC 3 cut(s) 122, 717, 737
BceAI ACGGC 3 cut(s) 127, 298, 849
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciT130I CCWGG 2 cut(s) 203, 569
BcoDI GTCTC 3 cut(s) 287, 368, 997
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 2 cut(s) 96, 816
BglII AGATCT 1 cut(s) 877
BisI GCNGC 4 cut(s) 55, 96, 821, 849
BlsI GCNGC 4 cut(s) 56, 97, 822, 850
Bme1390I CCNGG 2 cut(s) 203, 569
Bme18I GGWCC 5 cut(s) 199, 245, 255, 287, 901
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 5 cut(s) 199, 245, 255, 287, 901
BmiI GGNNCC 5 cut(s) 152, 246, 247, 256, 624
BmrFI CCNGG 2 cut(s) 203, 569
BmrI ACTGGG 1 cut(s) 983
BmsI GCATC 1 cut(s) 855
BmuI ACTGGG 1 cut(s) 983
BoxI GACNNNNGTC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 58
Bpu14I TTCGAA 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 781
BsaHI GRCGYC 1 cut(s) 907
BsaI GGTCTC 2 cut(s) 287, 368
BsaJI CCNNGG 1 cut(s) 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 806
Bse118I RCCGGY 1 cut(s) 625
Bse1I ACTGG 1 cut(s) 989
BseBI CCWGG 2 cut(s) 203, 569
BseDI CCNNGG 1 cut(s) 1015
BseGI GGATG 4 cut(s) 409, 700, 709, 796
BseLI CCNNNNNNNGG 1 cut(s) 806
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 1 cut(s) 989
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 82, 835
Bsh1236I CGCG 2 cut(s) 54, 173
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 2 cut(s) 219, 1009
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 3 cut(s) 81, 258, 268
BslI CCNNNNNNNGG 1 cut(s) 806
BsmAI GTCTC 3 cut(s) 287, 368, 997
BsmFI GGGAC 3 cut(s) 81, 258, 268
BsnI GGCC 1 cut(s) 206
Bso31I GGTCTC 2 cut(s) 287, 368
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 219, 987, 1009
Bsp143I GATC 4 cut(s) 223, 234, 514, 877
BspACI CCGC 9 cut(s) 54, 114, 194, 285, 297, 347, 417, 806, 820
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 84
BspFNI CGCG 2 cut(s) 54, 173
BspLI GGNNCC 5 cut(s) 152, 246, 247, 256, 624
BspPI GGATC 2 cut(s) 231, 509
BspQI GCTCTTC 1 cut(s) 975
BspT104I TTCGAA 1 cut(s) 428
BspTNI GGTCTC 2 cut(s) 287, 368
BsrBI CCGCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 625
BsrI ACTGG 1 cut(s) 989
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 1015
BssMI GATC 4 cut(s) 223, 234, 514, 877
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 907
BssSI CACGAG 1 cut(s) 1008
BssT1I CCWWGG 1 cut(s) 1015
Bst1107I GTATAC 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 1008
Bst2UI CCWGG 2 cut(s) 203, 569
Bst4CI ACNGT 4 cut(s) 62, 463, 931, 953
Bst6I CTCTTC 1 cut(s) 975
BstACI GRCGYC 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 428
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 4 cut(s) 409, 700, 709, 796
BstFNI CGCG 2 cut(s) 54, 173
BstH2I RGCGCY 2 cut(s) 96, 816
BstHHI GCGC 3 cut(s) 54, 95, 815
BstKTI GATC 4 cut(s) 226, 237, 517, 880
BstMAI GTCTC 3 cut(s) 287, 368, 997
BstMBI GATC 4 cut(s) 223, 234, 514, 877
BstMWI GCNNNNNNNGC 2 cut(s) 344, 812
BstNI CCWGG 2 cut(s) 203, 569
BstNSI RCATGY 2 cut(s) 608, 806
BstPAI GACNNNNGTC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 201, 567
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 2 cut(s) 54, 173
BstV1I GCAGC 2 cut(s) 82, 835
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
BstZ17I GTATAC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 4 cut(s) 409, 700, 709, 796
CfoI GCGC 3 cut(s) 54, 95, 815
Cfr10I RCCGGY 1 cut(s) 625
Cfr13I GGNCC 5 cut(s) 199, 245, 255, 287, 901
CseI GACGC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 169, 441
CviAII CATG 7 cut(s) 471, 488, 512, 539, 605, 664, 803
CviQI GTAC 2 cut(s) 169, 441
DdeI CTNAG 1 cut(s) 71
DpnI GATC 4 cut(s) 225, 236, 516, 879
DpnII GATC 4 cut(s) 223, 234, 514, 877
DraI TTTAAA 1 cut(s) 534
DrdI GACNNNNNNGTC 1 cut(s) 899
DseDI GACNNNNNNGTC 1 cut(s) 899
Eam1104I CTCTTC 1 cut(s) 975
EarI CTCTTC 1 cut(s) 975
EciI GGCGGA 2 cut(s) 129, 300
Eco130I CCWWGG 1 cut(s) 1015
Eco24I GRGCYC 1 cut(s) 987
Eco31I GGTCTC 2 cut(s) 287, 368
Eco47I GGWCC 5 cut(s) 199, 245, 255, 287, 901
Eco57I CTGAAG 1 cut(s) 866
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 245, 255
EcoRII CCWGG 2 cut(s) 201, 567
EcoT14I CCWWGG 1 cut(s) 1015
EcoT38I GRGCYC 1 cut(s) 987
ErhI CCWWGG 1 cut(s) 1015
FaeI CATG 7 cut(s) 474, 491, 515, 542, 608, 667, 806
FaqI GGGAC 3 cut(s) 81, 258, 268
FatI CATG 7 cut(s) 470, 487, 511, 538, 604, 663, 802
FauI CCCGC 1 cut(s) 799
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 4 cut(s) 55, 96, 821, 849
FokI GGATG 4 cut(s) 416, 687, 696, 783
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 4 cut(s) 55, 96, 821, 849
FspBI CTAG 1 cut(s) 444
GlaI GCGC 3 cut(s) 53, 94, 814
GluI GCNGC 4 cut(s) 55, 96, 821, 849
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 96, 816
HaeIII GGCC 1 cut(s) 206
HapII CCGG 1 cut(s) 626
HgaI GACGC 1 cut(s) 599
HhaI GCGC 3 cut(s) 54, 95, 815
Hin1I GRCGYC 1 cut(s) 907
Hin1II CATG 7 cut(s) 474, 491, 515, 542, 608, 667, 806
Hin6I GCGC 3 cut(s) 52, 93, 813
HinP1I GCGC 3 cut(s) 52, 93, 813
HinfI GANTC 7 cut(s) 19, 40, 69, 84, 239, 350, 719
HpaII CCGG 1 cut(s) 626
HphI GGTGA 2 cut(s) 74, 599
Hpy166II GTNNAC 3 cut(s) 271, 414, 790
Hpy188I TCNGA 8 cut(s) 155, 228, 398, 644, 742, 877, 885, 905
Hpy188III TCNNGA 5 cut(s) 243, 650, 917, 966, 1010
Hpy8I GTNNAC 3 cut(s) 271, 414, 790
Hpy99I CGWCG 5 cut(s) 62, 92, 266, 278, 909
HpyAV CCTTC 3 cut(s) 268, 401, 892
HpyCH4III ACNGT 4 cut(s) 62, 463, 931, 953
HpyCH4IV ACGT 2 cut(s) 306, 907
HpyCH4V TGCA 4 cut(s) 505, 608, 635, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 812
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 2 cut(s) 306, 907
Hsp92I GRCGYC 1 cut(s) 907
Hsp92II CATG 7 cut(s) 474, 491, 515, 542, 608, 667, 806
HspAI GCGC 3 cut(s) 52, 93, 813
KflI GGGWCCC 1 cut(s) 245
Kzo9I GATC 4 cut(s) 223, 234, 514, 877
LguI GCTCTTC 1 cut(s) 975
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 2 cut(s) 82, 835
LweI GCATC 1 cut(s) 855
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 306, 907
MaeIII GTNAC 4 cut(s) 364, 587, 953, 1010
MalI GATC 4 cut(s) 225, 236, 516, 879
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 223, 234, 514, 877
MboII GAAGA 3 cut(s) 119, 872, 992
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 3 cut(s) 219, 987, 1009
MluCI AATT 5 cut(s) 316, 522, 638, 783, 888
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 4 cut(s) 49, 63, 248, 359
MmeI TCCRAC 2 cut(s) 61, 928
MnlI CCTC 6 cut(s) 58, 110, 113, 214, 528, 714
MseI TTAA 4 cut(s) 533, 615, 828, 947
MslI CAYNNNNRTG 3 cut(s) 510, 714, 801
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 2 cut(s) 203, 569
MvaI CCWGG 2 cut(s) 203, 569
MvnI CGCG 2 cut(s) 54, 173
MwoI GCNNNNNNNGC 2 cut(s) 344, 812
NdeII GATC 4 cut(s) 223, 234, 514, 877
NlaIII CATG 7 cut(s) 474, 491, 515, 542, 608, 667, 806
NlaIV GGNNCC 5 cut(s) 152, 246, 247, 256, 624
NmuCI GTSAC 4 cut(s) 364, 587, 953, 1010
NspI RCATGY 2 cut(s) 608, 806
NspV TTCGAA 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 218
PciSI GCTCTTC 1 cut(s) 975
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 270
PfeI GAWTC 3 cut(s) 19, 84, 719
PkrI GCNGC 4 cut(s) 56, 97, 822, 850
PleI GAGTC 4 cut(s) 48, 63, 247, 358
PpsI GAGTC 4 cut(s) 48, 63, 247, 358
PpuMI RGGWCCY 2 cut(s) 245, 255
PshAI GACNNNNGTC 1 cut(s) 260
Psp5II RGGWCCY 2 cut(s) 245, 255
Psp6I CCWGG 2 cut(s) 201, 567
PspGI CCWGG 2 cut(s) 201, 567
PspN4I GGNNCC 5 cut(s) 152, 246, 247, 256, 624
PspPI GGNCC 5 cut(s) 199, 245, 255, 287, 901
PspPPI RGGWCCY 2 cut(s) 245, 255
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 2 cut(s) 170, 442
RsaNI GTAC 2 cut(s) 169, 441
RseI CAYNNNNRTG 3 cut(s) 510, 714, 801
SapI GCTCTTC 1 cut(s) 975
SaqAI TTAA 4 cut(s) 533, 615, 828, 947
SatI GCNGC 4 cut(s) 55, 96, 821, 849
Sau3AI GATC 4 cut(s) 223, 234, 514, 877
Sau96I GGNCC 5 cut(s) 199, 245, 255, 287, 901
SchI GAGTC 4 cut(s) 49, 63, 248, 359
ScrFI CCNGG 2 cut(s) 203, 569
SduI GDGCHC 3 cut(s) 219, 987, 1009
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 1 cut(s) 582
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 428
SinI GGWCC 5 cut(s) 199, 245, 255, 287, 901
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 3 cut(s) 510, 714, 801
SmlI CTYRAG 2 cut(s) 218, 760
SmoI CTYRAG 2 cut(s) 218, 760
Sse9I AATT 5 cut(s) 316, 522, 638, 783, 888
SsiI CCGC 9 cut(s) 54, 114, 194, 285, 297, 347, 417, 806, 820
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 201, 567
StyI CCWWGG 1 cut(s) 1015
TaaI ACNGT 4 cut(s) 62, 463, 931, 953
TaiI ACGT 2 cut(s) 309, 910
TaqI TCGA 4 cut(s) 219, 237, 301, 428
TasI AATT 5 cut(s) 316, 522, 638, 783, 888
TauI GCSGC 2 cut(s) 57, 823
TfiI GAWTC 3 cut(s) 19, 84, 719
Tru1I TTAA 4 cut(s) 533, 615, 828, 947
Tru9I TTAA 4 cut(s) 533, 615, 828, 947
TseFI GTSAC 4 cut(s) 364, 587, 953, 1010
TseI GCWGC 2 cut(s) 95, 848
Tsp45I GTSAC 4 cut(s) 364, 587, 953, 1010
TspDTI ATGAA 5 cut(s) 32, 420, 504, 614, 783
TspGWI ACGGA 3 cut(s) 291, 386, 416
VpaK11BI GGWCC 5 cut(s) 199, 245, 255, 287, 901
XapI RAATTY 1 cut(s) 638
XceI RCATGY 2 cut(s) 608, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 444
ZraI GACGTC 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.