RchiOBHm_Chr4g0425561

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008465
Physical Location & Seq
Forward (+)
51182653 .. 51186513
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39471

Sequence Viewer

Length: 1155 bp
ATGCTTCATGCATTGATGAATCTTCTCTCGCTGTGTTGGAAGCCATTTGGGCGCGGCGACGGTGAGGGAGGAGTCGACTCTATCGGAATTGTTGGCGCCAATTTAAAAGGCGGCGGTGGAGGAAGAGATGGCAAAGATAGCTTGCTTTGGTTCCGAGATATCGGAAAGTACGCCTGGGGAGATTTCTCCATGGCTGTGGTTCAGGCCAATCAGGTCCTCGAGGACCAGGGGCAGATCGAGTCCGGCCCCTTTGGGACCTTCGTCGGCGTCTATGATGGCCACGGTGGCCCGGAGGCCGCTCAATACGTCTGCGATAATCTATTTAGACATTTTCAAGTATCAGCGGAGACGGAGGATGTTGTGACAGCAGAGACCATTCAAAATGCTTTTCGTCAGACAGAGGAAGGATTTACGGCCCTTGTTTCTGAGTTGTGGAGTACTAAATCCAATTTAGCAACTGTAGGAACATGCTGTCTGGTTGGGGTGATATGTCAGGGGACCCTATTTGTGGCAAGTCTTGGAGATTCCCGTCTTGTTTTGGGGAAGAAAGTGGGCAACACTGGGGAAGTTGCTGCTATTCAGTTATCCACCGAACACAATGCGAATCTCGATGAGATTAGGTACGAGCTAAAAGAGCTGCACCCACATGATTCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGAATCATCCAGGTTTCTAGATCTATAGGTGATGTATATATGAAACATGCACAGTTTAACAGGGAGCCAATTGCTGCAAAATTCCGACTTCCTGAACCAATGAACATGCCTATCATGTCTGCCACTCCAACTATTCTTTCTCATGCTCTGCATCCAAATGATTCTTTTCTTATCTTTGCGTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAGATTGTCCACAAGCATCCTCATGCAGGAAGTGCCAAAAGGCTTGTTAAGACAGCGCTCCAAGAAGCTGCAAGAAAACGAGAAATGCGTTATTCAGATCTTCGGAAAATAGACAAGAAAGTGCGGCGCCATTTTCATGATGATATAACTGTTATTGTTTTATTCTTGAACCATGACCTAATCTCCAGAGGCACAGCACAAGAACCCCCAGTCTCAATAAGAAGTGGTCTTGATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

384

Amino Acids

42.28

Weight (kDa)

6.31

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 69 - 308 4.7e-06 Protein phosphatase 2C
PP2C PF00481 86 - 330 5.4e-35 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 218
AccB1I GGYRCC 2 cut(s) 95, 1041
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 54
AciI CCGC 6 cut(s) 54, 111, 114, 297, 344, 1039
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 773
AcyI GRCGYC 3 cut(s) 96, 267, 1042
AfaI GTAC 3 cut(s) 170, 439, 623
AfeI AGCGCT 1 cut(s) 972
AfiI CCNNNNNNNGG 2 cut(s) 508, 941
AgsI TTSAA 3 cut(s) 335, 380, 1084
AjnI CCWGG 3 cut(s) 173, 225, 702
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 5 cut(s) 141, 628, 637, 911, 983
AluI AGCT 5 cut(s) 141, 628, 637, 911, 983
Alw26I GTCTC 3 cut(s) 341, 365, 1132
Ama87I CYCGRG 1 cut(s) 218
Aor51HI AGCGCT 1 cut(s) 972
AoxI GGCC 6 cut(s) 204, 244, 277, 286, 294, 414
ApeKI GCWGC 4 cut(s) 572, 637, 767, 983
ApoI RAATTY 1 cut(s) 773
AspLEI GCGC 4 cut(s) 54, 98, 973, 1044
AspS9I GGNCC 7 cut(s) 214, 223, 245, 255, 287, 415, 498
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 74, 496, 734
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 4 cut(s) 214, 223, 255, 498
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 2 cut(s) 95, 1041
BbvI GCAGC 4 cut(s) 559, 624, 754, 970
BccI CCATC 3 cut(s) 122, 269, 872
BceAI ACGGC 1 cut(s) 429
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 3 cut(s) 175, 227, 704
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 3 cut(s) 341, 365, 1132
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 459, 717
BfoI RGCGCY 3 cut(s) 99, 974, 1045
BglI GCCNNNNNGGC 2 cut(s) 49, 285
BglII AGATCT 2 cut(s) 713, 1012
BisI GCNGC 8 cut(s) 55, 112, 297, 573, 638, 768, 984, 1040
BlsI GCNGC 8 cut(s) 56, 113, 298, 574, 639, 769, 985, 1041
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 4 cut(s) 175, 227, 290, 704
Bme18I GGWCC 4 cut(s) 214, 223, 255, 498
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 7 cut(s) 214, 223, 245, 255, 287, 415, 498
BmiI GGNNCC 8 cut(s) 97, 152, 247, 256, 499, 500, 759, 1043
BmrFI CCNGG 4 cut(s) 175, 227, 290, 704
BmrI ACTGGG 2 cut(s) 570, 1118
BmsI GCATC 2 cut(s) 853, 940
BmuI ACTGGG 2 cut(s) 570, 1118
BoxI GACNNNNGTC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 1084
BpuEI CTTGAG 1 cut(s) 916
BpuMI CCSGG 1 cut(s) 290
BsaHI GRCGYC 3 cut(s) 96, 267, 1042
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 4 cut(s) 174, 189, 226, 280
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 2 cut(s) 508, 941
Bse1I ACTGG 2 cut(s) 565, 1124
BseBI CCWGG 3 cut(s) 175, 227, 704
BseDI CCNNGG 4 cut(s) 174, 189, 226, 280
BseGI GGATG 4 cut(s) 361, 699, 844, 931
BseLI CCNNNNNNNGG 2 cut(s) 508, 941
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 565, 1124
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 4 cut(s) 559, 624, 754, 970
BsgI GTGCAG 1 cut(s) 623
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 6 cut(s) 206, 246, 279, 288, 296, 416
BshNI GGYRCC 2 cut(s) 95, 1041
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 243, 290
BslFI GGGAC 2 cut(s) 268, 511
BslI CCNNNNNNNGG 2 cut(s) 508, 941
BsmAI GTCTC 3 cut(s) 341, 365, 1132
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 268, 511
BsnI GGCC 6 cut(s) 206, 246, 279, 288, 296, 416
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 218
Bsp143I GATC 4 cut(s) 234, 713, 1012, 1147
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 6 cut(s) 54, 111, 114, 297, 344, 1039
BspANI GGCC 6 cut(s) 206, 246, 279, 288, 296, 416
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 8 cut(s) 97, 152, 247, 256, 499, 500, 759, 1043
BspT107I GGYRCC 2 cut(s) 95, 1041
BspTNI GGTCTC 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 2 cut(s) 565, 1124
BssECI CCNNGG 4 cut(s) 174, 189, 226, 280
BssMI GATC 4 cut(s) 234, 713, 1012, 1147
BssNI GRCGYC 3 cut(s) 96, 267, 1042
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 175, 227, 704
Bst4CI ACNGT 5 cut(s) 62, 284, 460, 747, 1066
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 3 cut(s) 96, 267, 1042
BstAPI GCANNNNNTGC 1 cut(s) 947
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 2 cut(s) 189, 280
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 4 cut(s) 361, 699, 844, 931
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 3 cut(s) 99, 974, 1045
BstHHI GCGC 4 cut(s) 54, 98, 973, 1044
BstKTI GATC 4 cut(s) 237, 716, 1015, 1150
BstMAI GTCTC 3 cut(s) 341, 365, 1132
BstMBI GATC 4 cut(s) 234, 713, 1012, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 49, 138, 285, 634, 947
BstNI CCWGG 3 cut(s) 175, 227, 704
BstNSI RCATGY 3 cut(s) 471, 743, 802
BstPAI GACNNNNGTC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 173, 225, 288, 702
BstSFI CTRYAG 2 cut(s) 459, 717
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 4 cut(s) 559, 624, 754, 970
BstX2I RGATCY 2 cut(s) 713, 1012
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 2 cut(s) 713, 1012
BsuRI GGCC 6 cut(s) 206, 246, 279, 288, 296, 416
BtgI CCRYGG 2 cut(s) 189, 280
BtsCI GGATG 4 cut(s) 361, 699, 844, 931
BtsIMutI CAGTG 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 143
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 4 cut(s) 54, 98, 973, 1044
Cfr13I GGNCC 7 cut(s) 214, 223, 245, 255, 287, 415, 498
CseI GACGC 2 cut(s) 256, 861
Csp6I GTAC 3 cut(s) 169, 438, 622
CviQI GTAC 3 cut(s) 169, 438, 622
DdeI CTNAG 1 cut(s) 426
DinI GGCGCC 2 cut(s) 97, 1043
DpnI GATC 4 cut(s) 236, 715, 1014, 1149
DpnII GATC 4 cut(s) 234, 713, 1012, 1147
DraI TTTAAA 2 cut(s) 105, 669
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 4 cut(s) 214, 223, 255, 498
Eco47III AGCGCT 1 cut(s) 972
Eco88I CYCGRG 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoO109I RGGNCCY 3 cut(s) 214, 255, 498
EcoRII CCWGG 3 cut(s) 173, 225, 702
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 13
EgeI GGCGCC 2 cut(s) 97, 1043
EheI GGCGCC 2 cut(s) 97, 1043
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 341
FaqI GGGAC 2 cut(s) 268, 511
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 8 cut(s) 55, 112, 297, 573, 638, 768, 984, 1040
FokI GGATG 4 cut(s) 368, 686, 831, 918
Fsp4HI GCNGC 8 cut(s) 55, 112, 297, 573, 638, 768, 984, 1040
FspBI CTAG 1 cut(s) 711
GlaI GCGC 4 cut(s) 53, 97, 972, 1043
GluI GCNGC 8 cut(s) 55, 112, 297, 573, 638, 768, 984, 1040
GsuI CTGGAG 1 cut(s) 1084
HaeII RGCGCY 3 cut(s) 99, 974, 1045
HaeIII GGCC 6 cut(s) 206, 246, 279, 288, 296, 416
HapII CCGG 2 cut(s) 243, 290
HgaI GACGC 2 cut(s) 256, 861
HhaI GCGC 4 cut(s) 54, 98, 973, 1044
Hin1I GRCGYC 3 cut(s) 96, 267, 1042
Hin6I GCGC 4 cut(s) 52, 96, 971, 1042
HinP1I GCGC 4 cut(s) 52, 96, 971, 1042
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 9 cut(s) 19, 72, 77, 239, 524, 604, 650, 696, 854
HpaII CCGG 2 cut(s) 243, 290
HphI GGTGA 3 cut(s) 74, 496, 734
Hpy166II GTNNAC 2 cut(s) 76, 925
Hpy188I TCNGA 9 cut(s) 86, 155, 164, 396, 427, 779, 877, 1012, 1020
Hpy188III TCNNGA 7 cut(s) 608, 711, 785, 1052, 1081, 1101, 1145
Hpy8I GTNNAC 2 cut(s) 76, 925
Hpy99I CGWCG 2 cut(s) 62, 266
HpyAV CCTTC 2 cut(s) 268, 398
HpyCH4III ACNGT 5 cut(s) 62, 284, 460, 747, 1066
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 7 cut(s) 11, 640, 743, 770, 844, 941, 986
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 138, 285, 634, 947
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 3 cut(s) 96, 267, 1042
HspAI GCGC 4 cut(s) 52, 96, 971, 1042
KasI GGCGCC 2 cut(s) 95, 1041
KflI GGGWCCC 1 cut(s) 498
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 4 cut(s) 234, 713, 1012, 1147
LmnI GCTCC 2 cut(s) 757, 978
Lsp1109I GCAGC 4 cut(s) 559, 624, 754, 970
LweI GCATC 2 cut(s) 853, 940
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 361
MalI GATC 4 cut(s) 236, 715, 1014, 1149
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 234, 713, 1012, 1147
MboII GAAGA 4 cut(s) 14, 135, 556, 1007
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 2 cut(s) 713, 1012
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 6 cut(s) 87, 100, 448, 657, 762, 773
MluNI TGGCCA 1 cut(s) 279
Mly113I GGCGCC 2 cut(s) 96, 1042
MlyI GAGTC 3 cut(s) 71, 81, 248
MmeI TCCRAC 3 cut(s) 17, 802, 845
Mox20I TGGCCA 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 1 cut(s) 279
MseI TTAA 4 cut(s) 104, 668, 750, 963
MslI CAYNNNNRTG 5 cut(s) 194, 645, 849, 936, 1050
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 2 cut(s) 243, 290
MspR9I CCNGG 4 cut(s) 175, 227, 290, 704
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 3 cut(s) 175, 227, 704
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 5 cut(s) 49, 138, 285, 634, 947
NarI GGCGCC 2 cut(s) 96, 1042
NciI CCSGG 1 cut(s) 290
NcoI CCATGG 1 cut(s) 189
NdeII GATC 4 cut(s) 234, 713, 1012, 1147
NlaIV GGNNCC 8 cut(s) 97, 152, 247, 256, 499, 500, 759, 1043
NmuCI GTSAC 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 471, 743, 802
PaeR7I CTCGAG 1 cut(s) 218
PagI TCATGA 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 1 cut(s) 1000
PfeI GAWTC 6 cut(s) 19, 524, 604, 650, 696, 854
PkrI GCNGC 8 cut(s) 56, 113, 298, 574, 639, 769, 985, 1041
PleI GAGTC 3 cut(s) 71, 80, 247
PluTI GGCGCC 2 cut(s) 99, 1045
PpsI GAGTC 3 cut(s) 71, 80, 247
PpuMI RGGWCCY 3 cut(s) 214, 255, 498
PshAI GACNNNNGTC 1 cut(s) 260
Psp5II RGGWCCY 3 cut(s) 214, 255, 498
Psp6I CCWGG 3 cut(s) 173, 225, 702
PspGI CCWGG 3 cut(s) 173, 225, 702
PspN4I GGNNCC 8 cut(s) 97, 152, 247, 256, 499, 500, 759, 1043
PspPI GGNCC 7 cut(s) 214, 223, 245, 255, 287, 415, 498
PspPPI RGGWCCY 3 cut(s) 214, 255, 498
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 2 cut(s) 713, 1012
RsaI GTAC 3 cut(s) 170, 439, 623
RsaNI GTAC 3 cut(s) 169, 438, 622
RseI CAYNNNNRTG 5 cut(s) 194, 645, 849, 936, 1050
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 4 cut(s) 104, 668, 750, 963
SatI GCNGC 8 cut(s) 55, 112, 297, 573, 638, 768, 984, 1040
Sau3AI GATC 4 cut(s) 234, 713, 1012, 1147
Sau96I GGNCC 7 cut(s) 214, 223, 245, 255, 287, 415, 498
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 3 cut(s) 71, 81, 248
ScrFI CCNGG 4 cut(s) 175, 227, 290, 704
SfaNI GCATC 2 cut(s) 853, 940
SfcI CTRYAG 2 cut(s) 459, 717
SfiI GGCCNNNNNGGCC 1 cut(s) 285
SfoI GGCGCC 2 cut(s) 97, 1043
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 4 cut(s) 214, 223, 255, 498
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 5 cut(s) 194, 645, 849, 936, 1050
SmlI CTYRAG 2 cut(s) 218, 895
SmoI CTYRAG 2 cut(s) 218, 895
Sse9I AATT 6 cut(s) 87, 100, 448, 657, 762, 773
SsiI CCGC 6 cut(s) 54, 111, 114, 297, 344, 1039
SspDI GGCGCC 2 cut(s) 95, 1041
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 173, 225, 288, 702
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 62, 284, 460, 747, 1066
TaiI ACGT 1 cut(s) 309
TaqI TCGA 4 cut(s) 75, 219, 237, 609
TasI AATT 6 cut(s) 87, 100, 448, 657, 762, 773
TatI WGTACW 1 cut(s) 437
TauI GCSGC 4 cut(s) 57, 114, 299, 1042
TfiI GAWTC 6 cut(s) 19, 524, 604, 650, 696, 854
Tru1I TTAA 4 cut(s) 104, 668, 750, 963
Tru9I TTAA 4 cut(s) 104, 668, 750, 963
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 572, 637, 767, 983
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 5 cut(s) 32, 749, 809, 918, 1040
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 565
VpaK11BI GGWCC 4 cut(s) 214, 223, 255, 498
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 1 cut(s) 773
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 3 cut(s) 471, 743, 802
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 711
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.