MD16G1152600.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008465
Physical Location & Seq
Reverse (-)
12030002 .. 12034015
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1152600.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGCTTCAGGCATTGATGAATCTGCTCTCTCTTTGCTGGAGGCCATTTGGGCGCGGCGACGGTGAGGGACTCAGCTCTGTTGGAATCGTCGGCGCTGCGAATAACGGAGGCGGCGGAGGAGGGCGAGATGGCAAAGATAACTTGCTTTGGTTCCGAGATTTTGGAAAGTACGCGTGGGGCGATTTCTCTATGGCGGTGGTCCAGGCCAATCAGGTGCTCGAGGATCAGAGCCAGATCGAGTCTGGACCCTTTGGGACCTTCGTCGGCGTATACGACGGACACGGCGGCCCGGAGACCGCTCGATACGTTTGCGACAATTTATTCAAACATTTCCAAGCAATATCAGTGGAGTCCGAAGGTGTTGTGACAGCGGAGACCATTCAAAATGCTTTTCTCCGAACAGAGGAAGGGTTTACCGCCCTTGTTTCAGAGCTGTGGGGTACTAGACCCCAAGTAGCAACTGTTGGGTCATGCTGTCTGGTTGGAGTTATATGTCAGGGGACCCTATTTGTGGCAAACCTGGGAGATTCCCGTTGTGTGTTGGGAAAGAAAGTGGGCAACACTGGGGAAGTTGCTGCGATCCAGTTATCCACTGAACACAATGCAAATCTCGAGGAGGTTAGGCATGAGCTGAAAGATTTGCACCCACACGATCCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGCATTATCCAGGTATCTAAATCTATAGGCGACGCATATATGAAACATGCAAGGTTTAACAGGGAGCCGATTAGTGCAAAATTCAGACTTCCCGAACCAATGACCATGCCTATCTTGTCTGCCGAACCAACCATTCTTTCTCATCCTCTCCATCCAAATGATTCTTTTCTTATATTTGCTTCTGATGGTCTATGGGAACACTTGAGTAATGAACAAGCTGTTGAAATTGTCCACAATCATCCACATTCAGGAAGCGCCAAGCGGCTTGTTAAGACGGCTCTCCAAGAAGCTGCAAGAAAAAGAGAGATGCGATATTCAGATCTTCGGAAAATTGACAAGAAGGTCCGACGCCATTTTCACGATGATATAACTGTCATTGTTTTATTCTTAAACCATGACCTAATATCCAGAGGGAGAGTGGAAGAGCCCCCAGTCTCAATAAAAAGTGCCCGTGACCAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

387

Amino Acids

42.51

Weight (kDa)

6.5

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 331 6.2e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 54, 173
AciI CCGC 9 cut(s) 54, 111, 114, 194, 285, 297, 371, 417, 958
AclWI GGATC 3 cut(s) 231, 574, 647
AcsI RAATTY 1 cut(s) 776
AcyI GRCGYC 1 cut(s) 1045
AfaI GTAC 2 cut(s) 170, 442
AfiI CCNNNNNNNGG 3 cut(s) 403, 511, 944
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 3 cut(s) 325, 383, 920
AjnI CCWGG 3 cut(s) 201, 519, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 5 cut(s) 75, 433, 631, 914, 986
AluI AGCT 5 cut(s) 75, 433, 631, 914, 986
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 3 cut(s) 287, 368, 1135
AlwI GGATC 3 cut(s) 231, 574, 647
Ama87I CYCGRG 2 cut(s) 218, 611
AoxI GGCC 3 cut(s) 41, 204, 286
ApeKI GCWGC 3 cut(s) 95, 575, 986
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 3 cut(s) 54, 95, 953
AspS9I GGNCC 6 cut(s) 199, 245, 255, 287, 501, 1039
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 218, 611
AvaII GGWCC 5 cut(s) 199, 245, 255, 501, 1039
BaeGI GKGCMC 1 cut(s) 1147
BanII GRGCYC 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 82, 562, 973
BccI CCATC 3 cut(s) 122, 855, 875
BceAI ACGGC 2 cut(s) 298, 987
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciT130I CCWGG 3 cut(s) 203, 521, 707
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 3 cut(s) 287, 368, 1135
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 720
BfoI RGCGCY 2 cut(s) 96, 954
BglI GCCNNNNNGGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 1015
BisI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
BlsI GCNGC 7 cut(s) 56, 97, 113, 287, 577, 960, 988
Bme1390I CCNGG 4 cut(s) 203, 290, 521, 707
Bme18I GGWCC 5 cut(s) 199, 245, 255, 501, 1039
BmeT110I CYCGRG 2 cut(s) 218, 611
BmgT120I GGNCC 6 cut(s) 199, 245, 255, 287, 501, 1039
BmiI GGNNCC 6 cut(s) 152, 247, 256, 502, 503, 762
BmrFI CCNGG 4 cut(s) 203, 290, 521, 707
BmrI ACTGGG 2 cut(s) 573, 1121
BmsI GCATC 1 cut(s) 993
BmuI ACTGGG 2 cut(s) 573, 1121
BoxI GACNNNNGTC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 58
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 1045
BsaI GGTCTC 2 cut(s) 287, 368
BsaJI CCNNGG 2 cut(s) 520, 1153
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 511, 944
Bse1I ACTGG 3 cut(s) 568, 583, 1127
BseBI CCWGG 3 cut(s) 203, 521, 707
BseDI CCNNGG 2 cut(s) 520, 1153
BseGI GGATG 3 cut(s) 838, 847, 934
BseLI CCNNNNNNNGG 3 cut(s) 403, 511, 944
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 3 cut(s) 568, 583, 1127
BseRI GAGGAG 2 cut(s) 132, 629
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 3 cut(s) 82, 562, 973
Bsh1236I CGCG 2 cut(s) 54, 173
BshFI GGCC 3 cut(s) 43, 206, 288
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 218, 611
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 3 cut(s) 81, 268, 514
BslI CCNNNNNNNGG 3 cut(s) 403, 511, 944
BsmAI GTCTC 3 cut(s) 287, 368, 1135
BsmFI GGGAC 3 cut(s) 81, 268, 514
BsnI GGCC 3 cut(s) 43, 206, 288
Bso31I GGTCTC 2 cut(s) 287, 368
BsoBI CYCGRG 2 cut(s) 218, 611
Bsp1286I GDGCHC 3 cut(s) 219, 1125, 1147
Bsp143I GATC 5 cut(s) 223, 234, 579, 652, 1015
BspACI CCGC 9 cut(s) 54, 111, 114, 194, 285, 297, 371, 417, 958
BspANI GGCC 3 cut(s) 43, 206, 288
BspCNI CTCAG 1 cut(s) 84
BspFNI CGCG 2 cut(s) 54, 173
BspLI GGNNCC 6 cut(s) 152, 247, 256, 502, 503, 762
BspPI GGATC 3 cut(s) 231, 574, 647
BspQI GCTCTTC 1 cut(s) 1113
BspTNI GGTCTC 2 cut(s) 287, 368
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 3 cut(s) 568, 583, 1127
BssECI CCNNGG 2 cut(s) 520, 1153
BssMI GATC 5 cut(s) 223, 234, 579, 652, 1015
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 1045
BssT1I CCWWGG 1 cut(s) 1153
Bst1107I GTATAC 1 cut(s) 271
Bst2UI CCWGG 3 cut(s) 203, 521, 707
Bst4CI ACNGT 3 cut(s) 62, 463, 1069
Bst6I CTCTTC 1 cut(s) 1113
BstACI GRCGYC 1 cut(s) 1045
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 3 cut(s) 838, 847, 934
BstFNI CGCG 2 cut(s) 54, 173
BstH2I RGCGCY 2 cut(s) 96, 954
BstHHI GCGC 3 cut(s) 54, 95, 953
BstKTI GATC 5 cut(s) 226, 237, 582, 655, 1018
BstMAI GTCTC 3 cut(s) 287, 368, 1135
BstMBI GATC 5 cut(s) 223, 234, 579, 652, 1015
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 3 cut(s) 203, 521, 707
BstNSI RCATGY 1 cut(s) 746
BstPAI GACNNNNGTC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 201, 288, 519, 705
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 1147
BstUI CGCG 2 cut(s) 54, 173
BstV1I GCAGC 3 cut(s) 82, 562, 973
BstX2I RGATCY 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 1015
BstZ17I GTATAC 1 cut(s) 271
BsuRI GGCC 3 cut(s) 43, 206, 288
BtsCI GGATG 3 cut(s) 838, 847, 934
BtsIMutI CAGTG 3 cut(s) 351, 561, 591
CfoI GCGC 3 cut(s) 54, 95, 953
Cfr13I GGNCC 6 cut(s) 199, 245, 255, 287, 501, 1039
CseI GACGC 2 cut(s) 737, 1053
Csp6I GTAC 2 cut(s) 169, 441
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 6 cut(s) 471, 626, 677, 743, 802, 1091
CviQI GTAC 2 cut(s) 169, 441
DdeI CTNAG 1 cut(s) 71
DpnI GATC 5 cut(s) 225, 236, 581, 654, 1017
DpnII GATC 5 cut(s) 223, 234, 579, 652, 1015
DraI TTTAAA 1 cut(s) 672
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
Eam1104I CTCTTC 1 cut(s) 1113
EarI CTCTTC 1 cut(s) 1113
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 1153
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 2 cut(s) 287, 368
Eco47I GGWCC 5 cut(s) 199, 245, 255, 501, 1039
Eco88I CYCGRG 2 cut(s) 218, 611
EcoO109I RGGNCCY 2 cut(s) 255, 501
EcoRII CCWGG 3 cut(s) 201, 519, 705
EcoT14I CCWWGG 1 cut(s) 1153
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 1 cut(s) 1153
FaeI CATG 6 cut(s) 474, 629, 680, 746, 805, 1094
FaqI GGGAC 3 cut(s) 81, 268, 514
FatI CATG 6 cut(s) 470, 625, 676, 742, 801, 1090
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
FokI GGATG 3 cut(s) 825, 834, 921
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
FspBI CTAG 1 cut(s) 444
GlaI GCGC 3 cut(s) 53, 94, 952
GluI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
GsuI CTGGAG 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 96, 954
HaeIII GGCC 3 cut(s) 43, 206, 288
HapII CCGG 1 cut(s) 290
HgaI GACGC 2 cut(s) 737, 1053
HhaI GCGC 3 cut(s) 54, 95, 953
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 6 cut(s) 474, 629, 680, 746, 805, 1094
Hin6I GCGC 3 cut(s) 52, 93, 951
HinP1I GCGC 3 cut(s) 52, 93, 951
HinfI GANTC 7 cut(s) 19, 69, 84, 239, 350, 527, 857
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 271, 414, 928
Hpy188III TCNNGA 6 cut(s) 243, 611, 788, 945, 1055, 1104
Hpy8I GTNNAC 3 cut(s) 271, 414, 928
Hpy99I CGWCG 6 cut(s) 62, 92, 266, 278, 731, 1047
HpyAV CCTTC 4 cut(s) 268, 350, 401, 1030
HpyCH4III ACNGT 3 cut(s) 62, 463, 1069
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 605, 643, 746, 773, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 6 cut(s) 474, 629, 680, 746, 805, 1094
HspAI GCGC 3 cut(s) 52, 93, 951
KflI GGGWCCC 1 cut(s) 501
Kzo9I GATC 5 cut(s) 223, 234, 579, 652, 1015
LguI GCTCTTC 1 cut(s) 1113
LmnI GCTCC 1 cut(s) 760
Lsp1109I GCAGC 3 cut(s) 82, 562, 973
LweI GCATC 1 cut(s) 993
MaeI CTAG 1 cut(s) 444
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 364, 1148
MalI GATC 5 cut(s) 225, 236, 581, 654, 1017
MbiI CCGCTC 1 cut(s) 299
MboI GATC 5 cut(s) 223, 234, 579, 652, 1015
MboII GAAGA 2 cut(s) 1010, 1130
MflI RGATCY 1 cut(s) 1015
MhlI GDGCHC 3 cut(s) 219, 1125, 1147
MluCI AATT 5 cut(s) 316, 660, 776, 921, 1026
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 3 cut(s) 63, 248, 359
MmeI TCCRAC 3 cut(s) 61, 463, 1066
MseI TTAA 4 cut(s) 671, 753, 966, 1085
MslI CAYNNNNRTG 1 cut(s) 852
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 4 cut(s) 203, 290, 521, 707
MvaI CCWGG 3 cut(s) 203, 521, 707
MvnI CGCG 2 cut(s) 54, 173
MwoI GCNNNNNNNGC 1 cut(s) 49
NciI CCSGG 1 cut(s) 290
NdeII GATC 5 cut(s) 223, 234, 579, 652, 1015
NlaIII CATG 6 cut(s) 474, 629, 680, 746, 805, 1094
NlaIV GGNNCC 6 cut(s) 152, 247, 256, 502, 503, 762
NmuCI GTSAC 2 cut(s) 364, 1148
NspI RCATGY 1 cut(s) 746
PaeR7I CTCGAG 2 cut(s) 218, 611
PciSI GCTCTTC 1 cut(s) 1113
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 270
PfeI GAWTC 4 cut(s) 19, 84, 527, 857
PkrI GCNGC 7 cut(s) 56, 97, 113, 287, 577, 960, 988
PleI GAGTC 3 cut(s) 63, 247, 358
PpsI GAGTC 3 cut(s) 63, 247, 358
PpuMI RGGWCCY 2 cut(s) 255, 501
PshAI GACNNNNGTC 1 cut(s) 260
Psp5II RGGWCCY 2 cut(s) 255, 501
Psp6I CCWGG 3 cut(s) 201, 519, 705
PspGI CCWGG 3 cut(s) 201, 519, 705
PspN4I GGNNCC 6 cut(s) 152, 247, 256, 502, 503, 762
PspPI GGNCC 6 cut(s) 199, 245, 255, 287, 501, 1039
PspPPI RGGWCCY 2 cut(s) 255, 501
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 1 cut(s) 1015
RsaI GTAC 2 cut(s) 170, 442
RsaNI GTAC 2 cut(s) 169, 441
RseI CAYNNNNRTG 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 1113
SaqAI TTAA 4 cut(s) 671, 753, 966, 1085
SatI GCNGC 7 cut(s) 55, 96, 112, 286, 576, 959, 987
Sau3AI GATC 5 cut(s) 223, 234, 579, 652, 1015
Sau96I GGNCC 6 cut(s) 199, 245, 255, 287, 501, 1039
SchI GAGTC 3 cut(s) 63, 248, 359
ScrFI CCNGG 4 cut(s) 203, 290, 521, 707
SduI GDGCHC 3 cut(s) 219, 1125, 1147
SfaNI GCATC 1 cut(s) 993
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 2 cut(s) 218, 611
SinI GGWCC 5 cut(s) 199, 245, 255, 501, 1039
SlaI CTCGAG 2 cut(s) 218, 611
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 3 cut(s) 218, 611, 898
SmoI CTYRAG 3 cut(s) 218, 611, 898
Sse9I AATT 5 cut(s) 316, 660, 776, 921, 1026
SsiI CCGC 9 cut(s) 54, 111, 114, 194, 285, 297, 371, 417, 958
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 4 cut(s) 201, 288, 519, 705
StyI CCWWGG 1 cut(s) 1153
TaaI ACNGT 3 cut(s) 62, 463, 1069
TaiI ACGT 1 cut(s) 309
TaqI TCGA 4 cut(s) 219, 237, 301, 612
TasI AATT 5 cut(s) 316, 660, 776, 921, 1026
TauI GCSGC 4 cut(s) 57, 114, 288, 961
TfiI GAWTC 4 cut(s) 19, 84, 527, 857
Tru1I TTAA 4 cut(s) 671, 753, 966, 1085
Tru9I TTAA 4 cut(s) 671, 753, 966, 1085
TscAI CASTG 3 cut(s) 351, 568, 598
TseFI GTSAC 2 cut(s) 364, 1148
TseI GCWGC 3 cut(s) 95, 575, 986
Tsp45I GTSAC 2 cut(s) 364, 1148
TspDTI ATGAA 3 cut(s) 32, 752, 921
TspGWI ACGGA 2 cut(s) 120, 291
TspRI CASTG 3 cut(s) 351, 568, 598
VpaK11BI GGWCC 5 cut(s) 199, 245, 255, 501, 1039
XapI RAATTY 1 cut(s) 776
XceI RCATGY 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 239, 1111
XhoI CTCGAG 2 cut(s) 218, 611
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.